CN116590154B - Biocontrol bacterium for preventing and controlling plant root rot caused by soil-borne pathogenic bacteria and application thereof - Google Patents
Biocontrol bacterium for preventing and controlling plant root rot caused by soil-borne pathogenic bacteria and application thereof Download PDFInfo
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- C12N1/14—Fungi; Culture media therefor
- C12N1/145—Fungal isolates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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Abstract
The invention discloses a biocontrol bacterium for preventing and treating plant root rot caused by soil-borne pathogenic bacteria and application thereof, wherein the biocontrol bacterium is named as compact basket-shaped bacterium PK-1, and the biocontrol bacterium is prepared from the following components in a preservation unit: the China general microbiological culture Collection center (CGMCC) has a collection number of CGMCC NO.40517. The strain can obviously reduce the disease severity of root rot caused by various soil-borne pathogenic bacteria, wherein the pathogenic bacteria comprise one or more of phytophthora sojae, pythium ultimum, fusarium oxysporum, fusarium solani, fusarium equisetum, fusarium graminearum, rhizoctonia solani, aschersonia phaseoloides and phomopsis. The biocontrol microbial inoculum prepared by utilizing the compact basket fungus PK-1 is applied around the rhizosphere of the plant, so that the growth indexes such as the germination rate, the plant height, the fresh weight and the like of the plant can be obviously improved.
Description
Technical Field
The invention relates to the technical field of agricultural science, in particular to biocontrol bacteria for preventing and controlling plant root rot caused by soil-borne pathogenic bacteria and application thereof.
Background
Root rot is a common soil-borne disease in agricultural production that can occur at various stages of plant development. When root rot occurs, the plant root system is underdeveloped, the development is retarded, overground parts are dwarfed, the emaciation is weak, leaves fade, and branches and fruits are obviously reduced. In the early stage of root rot, only individual lateral roots and fibrous roots develop symptoms and gradually spread to main roots. After the main root is infected, early symptoms are not obvious, and then the plant moisture and nutrient absorption function is affected along with the aggravation of the root rot degree, so that the plant is yellow and wilted. In the later stage of the disease, the root cortex changes color, decays and separates from the medulla, and the plant wilts and dries up and dies. Root rot occurs in almost all places and various crops in China, and yield is reduced or is disabled in severe areas.
Taking soybean root rot as an example, soybean root rot is caused by various pathogenic bacteria, and at present, the pathogenic bacteria of soybean root rot which have been reported in China mainly include: phytophthora sojaePhytophthora sojae) Back-end mouldPythium ultimum) Fusarium oxysporum (Fusarium oxysporum)Fusarium oxysporum) Fusarium solani (Fusarium solani)Fusarium solani) Fusarium graminearumFusarium graminearum) Fusarium equisetumFusarium equiseti) Rhizoctonia solani @Rhizoctonia solani) Phomopsis is preparedPhomopsis longicolla) And Eichhornia crassipesMacrophomina phaseolina) Etc. In addition, the pathogenic species responsible for soybean root rot are not only complex, they can often also complex infestations, resulting in more serious yield losses. It is counted that more than 30% of soybeans are reduced in yield per year due to root rot, and even in severe cases, are subjected to harvest-control.
At present, the main control mode of soybean root rot is as follows: 1. planting disease-resistant varieties, and using disease-resistant varieties is the most economical, safe and effective measure for preventing and controlling diseases in soybean production. However, the resistance of soybean varieties is easy to lose due to complex pathogenic bacteria pathogenic groups and quick virulence variation; 2. the chemical control method is simple to operate and takes effect quickly, but pesticide residues can cause threat to food safety.
The natural environment around the plant is rich in microorganisms, and part of microorganisms can promote plant growth and help the plant resist infection of pathogenic bacteria, and can directly antagonize the pathogenic bacteria or induce plant resistance to resist the pathogenic bacteria, so that the beneficial microorganisms can improve the capability of the plant to resist biotic or abiotic stress. Furthermore, these strains isolated from the original ecological locus may have a more prolonged biocontrol potential against pathogenic bacteria. Nowadays, finding beneficial microorganisms in the environment to control plant diseases is increasingly being appreciated by researchers.
In view of the above, the prior art also lacks a green, efficient environmental probiotic for preventing root rot.
Disclosure of Invention
Aiming at the problems, the invention provides a biocontrol bacterium for preventing and treating root rot caused by soil-borne pathogenic bacteria and application thereof.
The first aspect of the invention provides a biocontrol bacterium for preventing and treating root rot caused by soil-borne pathogenic bacteria, which is characterized in that the biocontrol bacterium is named as compact basket-shaped bacterium PK-1, and the biocontrol bacterium is stored in a unit of: china general microbiological culture Collection center (CGMCC) with a collection number of CGMCC NO.40517.
The compact basket PK-1 is named as taxonomicTalaromyces adpressusThe collection and preservation of the strain in China general microbiological culture collection center (CGMCC) are registered and preserved in 2023, 03 and 06 days, the preservation number is CGMCC NO.40517, and the preservation address is North Chenxi Lu No.1 and 3 in the Chaoyang area of Beijing city.
The Phlebsiella compacta PK-1 was selected from soybean fields in Jiang Ning region of Nanjing.
In a second aspect, the invention provides a biocontrol microbial inoculum for preventing and treating plant root rot caused by soil-borne pathogenic bacteria, wherein the active ingredients of the biocontrol microbial inoculum comprise one or more of hyphae, spores or secondary metabolites of the compact basket fungus PK-1.
In certain embodiments, the spore content of the compact basket PK-1 is not less than 1×10 6 Each/g.
In certain embodiments, the mycelium of the Phlebsiella compacta PK-1 is prepared by inoculating the Phlebsiella compacta PK-1 into PDA culture medium for culture.
In a third aspect, the invention provides a preparation method of the biocontrol microbial agent, which comprises the following steps:
s1, mixing corn flour, rice straw and humic acid to prepare a matrix;
s2, adding hypha and spore of the claystis compactus PK-1 into the matrix;
s3, adding sterile water to enable the water content of the mixture obtained in the step S2 to be 50% -60%;
and S4, sealing and culturing the product obtained in the step S3 for a plurality of days to obtain the biocontrol microbial agent.
In certain embodiments, the method comprises the steps of:
a1: the basket was inoculated on PDA medium of diameter 9 cm and incubated at 25℃in the dark for 14 d until the dishes were full.
A2: sterilizing matrix (corn flour: rice straw: humic acid=6:2:2) at 121deg.C for 20 min, cooling, and packaging into sterile tray. Each tray of substrate 200 g.
A3: washing out the basket fungus spores on the PDA culture medium with sterile water, adding into the matrix, regulating water content to 60%, sealing, and culturing in dark at 28deg.C in an incubator for 21 d to obtain the solid fermentation inoculant for basket fungus.
In a fourth aspect of the present invention, there is provided a microbial pesticide whose active ingredient comprises the biocontrol bacterium or any of the microbial agents.
In a fifth aspect, the invention provides an application of the biocontrol bacterium, the biocontrol microbial agent or the microbial pesticide in preventing and treating plant root rot.
In certain embodiments, the root rot includes, but is not limited to, soybean root rot, tomato root rot, strawberry root rot, and tobacco root rot.
In certain embodiments, the root rot includes a root rot caused by one or more bacteria of the group consisting of phytophthora sojae, pythium ultimum, fusarium oxysporum, fusarium solani, fusarium equisetosum, fusarium graminearum, rhizoctonia solani, eichhornia crassipes, and Phomopsis.
In a sixth aspect of the present invention, there is provided a method for controlling plant root rot, comprising applying the biocontrol bacterium, the biocontrol agent or the microbial pesticide to a plant growth environment.
In certain embodiments, the plant growth environment is at the growth of the hypocotyl of a plant seedling.
In certain embodiments, the soil borne pathogenic bacteria include one or more of phytophthora sojae, pythium ultimum, fusarium oxysporum, fusarium solani, fusarium equisetum, fusarium graminearum, rhizoctonia solani, eichhornia crassipes, and Phomopsis.
In certain embodiments, the plant root rot includes soybean root rot, tomato root rot, strawberry root rot, and tobacco root rot.
Compared with the prior art, the invention provides a biocontrol bacterium for preventing and treating root rot caused by soil-borne pathogenic bacteria, namely the compact basket-shaped bacterium PK-1, which can obviously reduce the disease severity of root rot caused by various soil-borne pathogenic bacteria, wherein the pathogenic bacteria comprise one or more of phytophthora sojae, pythium ultimum, fusarium oxysporum, fusarium solani, fusarium equisetum, fusarium graminearum, rhizoctonia solani, bean curd ball and phomopsis. The biocontrol microbial inoculum prepared by utilizing the compact basket fungus PK-1 is applied around the rhizosphere of the plant, so that the growth indexes such as the germination rate, the plant height, the fresh weight and the like of the plant can be obviously improved.
Drawings
FIG. 1 shows the antagonistic effect of the fungus Penicillium compactum PK-1 of example 1 on different root rot pathogens;
FIG. 2 shows the effect of the fungus Penicillium compactum PK-1 of example 2 on root rot caused by different root rot fungi.
Detailed Description
The following examples facilitate a better understanding of the present invention, but are not intended to limit the same. The experimental methods in the following examples are conventional methods unless otherwise specified. The test materials used in the examples described below, unless otherwise specified, were purchased from conventional biochemical reagent stores.
As used herein, the term "biocontrol bacteria" refers to beneficial microorganisms, mainly bacteria, fungi, actinomycetes, which control plant diseases.
As described in the present invention, the term "engineering bacteria" refers to novel microorganisms processed by modern biological engineering technology, and has the characteristics of multifunction, high efficiency, strong adaptability, etc.
As used herein, the term "phytophthora sojae" is classified as: algae boundaryChromista) OrnithymenOomycota) Orthosiphon of the order PeronosporalesPeronosporales) Humicola plant (Pythiaceae)Pythiacea) Phytophthora genusPhytophthora). Is mainly distributed in the united states, canada, brazil, argentina, japan, australia, united kingdom, hungary, nigeria, india, china, egypt, israel, south africa, germany, swiss, new zealand and other countries.
As described in the present invention, the term "Pythium ultimum" belongs to the genus PythiumChromista) OrnithymenOomycota) Orthosiphon of the order PeronosporalesPeronosporales) Humicola plant (Pythiaceae)Pythiacea) Pythium speciesPythium) Colonies were radial on CMA. Developed hypha and luxuriant branches, and the thickness is 2.3-9.8 μm. The plant is originally separated from the rotten cress seedlings in the United kingdom, has very wide report distribution, and not only inhabits in soil, but also widely infects more than 150 economic plants such as soybean, bean, pea, sweet potato, pine seedling, coffee, apple, orange, peach, cotton, chrysanthemum, dahlia, pumpkin, watermelon, sugarcane, alfalfa, tomato and the like, and causes various diseases such as seedling withering, cataplexy, root rot, foot rot, wilt and the like.
As described in the present invention, the terms "Fusarium oxysporum", "Fusarium solani", "Fusarium equisetum" and "Fusarium graminearum" are belonging to the genus FusariumImperfecti fungi) From the order of pedunculaceaeMoniliales) Tuberosa of OenotheraceaeTuberculariaceae) Fusarium genusFusarium). Is a soil-borne pathogenic fungus distributed worldwide, has wide host range and can cause the occurrence of plant wilt of more than 100 plants such as melons, solanaceae, bananas, cottons, leguminous plants, flowers and the like.
As used in the present invention, the term "Rhizoctonia solani" belongs to the genus RhizoctoniaImperfecti fungi) The plant is of the order of no sporeAgonomycetales) Radix et rhizoma Rhei AchilleaAgonomycetaceae) Rhizoctonia genusRhizoctonia). Is a host modelThe plant soil-borne pathogenic fungi are widely spread, have strong saprophytic property and are widely distributed worldwide.
As used herein, the term "Eichhornia crassipes" belongs to the order Eichhornia, family Eichhorniaceae, and is a broad host range.
As used herein, the term "Phomopsis" refers to the order of the genus Shell mould, the family of the family Shell mould, and is widely distributed.
EXAMPLE 1 screening of the fungus Penicillium compactum PK-1
1. And (3) primary screening: isolation of potential biocontrol bacteria
1.1, obtaining a test material: soybean plants with good growth vigor are selected from fields with perennial root rot, and root tissues and rhizosphere soil are taken.
1.2, isolation and culture of soybean root microorganisms: cutting root tissue of soybean, washing with sterile water for 3 times, cutting the washed root tissue into small segments of 0.5-1 cm by a sterilizing scalpel, sucking the surface water of the root tissue with sterile absorbent paper, placing on PDA agar solid medium containing ampicillin, rifampicin and pentachloronitrobenzene, and culturing in a culture box at 25deg.C for 2 days.
1.3, isolation and culture of soybean rhizosphere soil microorganisms: soil was removed with sterilized forceps at 0.25. 0.25 g, placed on PDA agar solid medium containing ampicillin, rifampicin and pentachloronitrobenzene, and placed in an incubator at 25℃for 2 days. After hyphae grow out around the sample, hyphae blocks with the size of 2×2 mm are cut and placed in a new PDA solid culture medium with ampicillin, rifampicin and pentachloronitrobenzene for 2 days for culture again.
2. Screening for antagonistic bacteria by plate counter method
The single spore purified strain and common root rot pathogen are symmetrically inoculated at the position, which is 1 to cm away from the edge, of the culture medium plate by adopting a PDA plate confronting method. The plates were incubated at 25℃in the dark. And (5) observing the opposite result, and finally screening. As can be seen from fig. 1, the antagonistic effect of the antagonistic bacteria on root rot pathogen.
Morphological identification of antagonistic bacteria
The culture medium used for the experiment was as follows:
potato Dextrose Agar (PDA) medium: removing skin from potato 200 g, cutting into small pieces, adding water 1L, boiling for 10 min, filtering with gauze, adding 20 g glucose and 15 g agar, and fixing volume to 1L. Sterilizing at 121deg.C for 20 min.
Inoculating the separated antagonistic strain onto a flat plate, culturing in an incubator at 25 ℃ in an inverted manner, and observing the growth condition of the strain. The bacterial colony is round, the center of the mycelium is green, the edge is white, the floccule and the velvet are in a shape of symmetrical axillary branch of the conidiophore, and the conidiophore is spherical or nearly spherical.
4. Molecular biological identification of the resulting strains
4.1, placing the purified microorganism culture in PDA liquid culture medium, culturing for two days at 25 ℃, centrifuging the culture solution and collecting mycelium.
4.2, extracting the genome DNA of the collected mycelium by using a genome DNA rapid extraction kit of Tiangen biochemical technology Co.
4.3, amplifying the ITS2 (internal transcribed spacer 2) and RBP2 ((the second largest subunit of RNA polymerase II) genes of the genomic DNA obtained in the step 4.2 by using a PCR amplification kit of Nanjinouzan biotechnology Co., ltd.
4.4, after the PCR product obtained by amplification is subjected to 1% agarose gel electrophoresis, observing under an ultraviolet lamp, and sending the PCR product with a target band to Nanjing biological engineering Co., ltd for sequencing, wherein the sequencing result is as follows: the ITS2 gene sequence of the strain obtained by screening in the step 4 is SEQ ID NO.1, and the RBP2 gene sequence is SEQ ID NO.2.
4.5, two sequences of SEQ ID NO.1 and SEQ ID NO.2 were aligned on NCBI database website. The comparison results are shown in Table 1. Based on the results of Table 1, it can be estimated that the biocontrol strain of the present invention is a compact basket-like strainTalaromyces adpressus) Designated as the fungus compact basket fungus PK-1.
Example 2 Effect of Penicillium compactum PK-1 on root rot caused by different soil borne pathogens
The inventors of the present invention conducted a number of experiments on the effect of the fungus compact basket PK-1 screened in example 1 on root rot caused by different root rot fungi, and exemplified below by Phytophthora sojae, pythium ultimum, fusarium oxysporum, fusarium solani, fusarium equisetum, fusarium graminearum, rhizoctonia solani, eichhornia crassipes and Phomopsis.
[ Phytophthora sojae ]
The method for testing the effect of the compact basket fungus PK-1 on the root rot caused by the phytophthora sojae comprises the following steps:
2.1 inoculating Phytophthora sojae P6497 to the strain containing the strain PK-1 and the strain having a diameter of 9 cm
20 In a culture dish of mL PDA and 10% unfiltered V8 medium, 7 d was incubated at 25℃in the dark until the strain had grown over the dish.
2.2 selecting pot with proper size, cutting the culture medium full of the compact basket-shaped bacteria PK-1 and the phytophthora sojae P6497 into flat plates of 0.5 cm multiplied by 0.5 cm. Test set 2, control: 2V 8 plates of 0.5 cm ×0.5 cm and 2 plate blocks of 0.5 cm ×0.5 cm P.sojae 6497 were added to each pot; the experimental group is: 2 plates of P.sojae P6497 of 0.5 cm X0.5 cm and 2 plates of P.compactum PK-1 of 0.5 cm X0.5 cm were added to each pot. Each set 3 replicates.
2.3 stirring the 2 kinds of flat plate blocks uniformly with sterile water and sterilized vermiculite, adding the mixture into a pot to 1/3 of the position, sowing 5 soybeans (variety Hefeng 47) in each pot, and then covering the soybeans with flat plate powder.
2.4, irrigating each group of soybeans with sterile water, and counting the germination rate, plant height and fresh weight of the soybeans after sowing 14 and d. The results are shown in FIG. 2 and Table 2.
The numerical expression modes of the emergence rate, the plant height and the fresh weight in the table are as follows: mean ± standard error.
As can be seen from fig. 2, the plant height, the budding number and the flourishing degree of the experimental group are obviously better than those of the control group, and the fungus compact basket fungus PK-1 has the effect of preventing or curing the root rot caused by phytophthora sojae P6497.
The emergence rate of the experimental group in table 2 was increased by about 50% compared with the control group, and the fresh weight was also significantly increased.
From the results shown in fig. 2 and table 2, the isolated biocontrol strain compact basket fungus PK-1 has remarkable control effect on root rot caused by phytophthora sojae. The compact basket-shaped bacteria PK-1 treatment can obviously improve the emergence rate, plant height and fresh weight of the soybeans.
[ finally, pythium ]
And (3) detecting the influence of the fungus compact basket fungus PK-1 on the root rot caused by Pythium ultimum by adopting the method of the steps 2.1-2.4. In the experiment, phytophthora sojae P6497 was replaced by pythium ultimum Pyu. The test results are shown in fig. 2 and table 3.
From fig. 2, the budding amount and the flourishing degree of the experimental group are obviously better than those of the control group, and the fungus compact basket fungus PK-1 has the effect of preventing or curing root rot caused by Pythium ultimum.
In Table 3, the emergence rate of the experimental group is 86.67+ -11.55, the emergence rate of the control group is 13.33+ -11.55, the emergence rate of the experimental group is increased by multiple times relative to the control group, and the plant height and fresh weight are also obviously increased.
From the results shown in fig. 2 and table 3, the biocontrol strain compact basket fungus PK-1 has a remarkable control effect on root rot caused by Pythium ultimum, and the compact basket fungus PK-1 treatment remarkably improves the emergence rate and fresh weight of soybeans.
[ Fusarium oxysporum ]
And (3) detecting the influence of the fungus compact basket fungus PK-1 on the root rot caused by fusarium oxysporum by adopting the method of the steps 2.1-2.4. In the experiment, phytophthora sojae P6497 was replaced by Fusarium oxysporum. The test results are shown in table 4 of fig. 2.
From fig. 2, the budding quantity and the flourishing degree of the experimental group are obviously better than those of the control group, and the fungus compact basket fungus PK-1 has the effect of preventing or curing root rot caused by fusarium oxysporum.
The emergence rate and fresh weight of the experimental group in table 4 are significantly better than those of the control group.
From the results shown in fig. 2 and table 4, the biocontrol strain compact basket fungus PK-1 has a remarkable control effect on root rot caused by fusarium oxysporum, and the compact basket fungus PK-1 treatment remarkably improves the emergence rate and fresh weight of soybeans.
[ Fusarium solani ]
And (3) detecting the influence of the fungus compact basket fungus PK-1 on the root rot caused by the fusarium solani by adopting the method of the steps 2.1-2.4. In the experiment, phytophthora sojae P6497 was replaced by Fusarium solani. The test results are shown in table 5 of fig. 2.
From fig. 2, the plant height, the budding number and the flourishing degree of the experimental group are obviously better than those of the control group, and the fungus compact basket fungus PK-1 has the effect of preventing or curing the root rot caused by the fusarium solani.
The emergence rate, plant height and fresh weight of the experimental group in table 5 are significantly better than those of the control group.
From the results shown in fig. 2 and table 5, the biocontrol strain compact basket fungus PK-1 has a remarkable control effect on root rot caused by fusarium solani, and the compact basket fungus PK-1 treatment remarkably improves the emergence rate and fresh weight of soybeans.
[ Fusarium equisetum ]
And (3) detecting the influence of the fungus compact basket fungus PK-1 on the root rot caused by the fusarium equiseti by adopting the method of the steps 2.1-2.4. In the experiment, phytophthora sojae P6497 was replaced with Fusarium equisetum. The test results are shown in table 6 of fig. 2.
From fig. 2, the plant height, the budding number and the flourishing degree of the experimental group are obviously better than those of the control group, and the fungus compact basket fungus PK-1 has the effect of preventing or curing root rot caused by fusarium equiseti.
The emergence rate, plant height and fresh weight of the experimental group in table 6 are significantly better than those of the control group.
From the results shown in fig. 2 and table 6, the biocontrol strain compact basket fungus PK-1 has a remarkable control effect on root rot caused by fusarium equiseti, and the compact basket fungus PK-1 treatment remarkably improves the emergence rate and fresh weight of soybeans.
Fusarium graminearum
And (3) detecting the influence of the fungus compact basket fungus PK-1 on the root rot caused by the fusarium graminearum by adopting the method of the steps 2.1-2.4. In the experiment, phytophthora sojae P6497 was exchanged for Fusarium graminearum. The test results are shown in table 7 of fig. 2.
From fig. 2, the plant height, the budding number and the flourishing degree of the experimental group are obviously better than those of the control group, and the fungus compact basket fungus PK-1 has the effect of preventing or curing the root rot caused by fusarium graminearum.
The emergence rate, plant height and fresh weight of the experimental group in table 7 are significantly better than those of the control group.
From the results shown in fig. 2 and table 7, the biocontrol strain compact basket fungus PK-1 has a remarkable control effect on root rot caused by fusarium graminearum, and the compact basket fungus PK-1 treatment remarkably improves the emergence rate and fresh weight of soybeans.
[ Phomopsis ]
And (3) detecting the influence of the fungus compact basket fungus PK-1 on the root rot caused by the phomopsis by adopting the method of the steps 2.1-2.4. In the experiment, phytophthora sojae P6497 was exchanged for phomopsis. The test results are shown in fig. 2 and table 8.
From fig. 2, the plant height, the bud number and the flourishing degree of the experimental group are obviously better than those of the control group, and the fungus compact basket fungus PK-1 has the effect of preventing or curing the root rot caused by phomopsis.
In Table 8, the emergence rate of the experimental group was increased by about 75% compared with the control group, the plant height of the experimental group was increased by about 20% compared with the control group, and the fresh weight was also significantly increased.
From the results shown in fig. 2 and table 8, the biocontrol strain compact basket fungus PK-1 has remarkable control effect on root rot caused by phomopsis, and the compact basket fungus PK-1 treatment remarkably improves the emergence rate, plant height and fresh weight of soybeans.
The above description of the specific embodiments of the present invention has been given by way of example only, and the present invention is not limited to the above described specific embodiments. Any equivalent modifications and substitutions for the present invention will occur to those skilled in the art, and are also within the scope of the present invention. Accordingly, equivalent changes and modifications are intended to be included within the scope of the present invention without departing from the spirit and scope thereof.
Claims (8)
1. A biocontrol bacterium for preventing and treating root rot caused by soil-borne pathogenic bacteria is characterized in that the biocontrol bacterium is named as compact basket-shaped bacterium @Talaromyces adpressus) PK-1, accession number: china general microbiological culture Collection center (CGMCC) with a collection number of CGMCC NO.40517.
2. A biocontrol microbial inoculum for preventing and treating plant root rot caused by soil-borne pathogenic bacteria, which is characterized in that the active ingredients of the biocontrol microbial inoculum comprise the compact basket-shaped bacteria of claim 1Talaromyces adpressus) Hyphae or spores of PK-1.
3. The biocontrol microbial agent of claim 2, wherein said compacted basket isTalaromyces adpressus) The spore content of PK-1 is not less than 1×10 6 Each/g.
4. The biocontrol microbial agent of claim 3, wherein said compacted basket isTalaromyces adpressus) The mycelium of PK-1 is prepared from the strain of Penicillium compactumTalaromyces adpressus) The PK-1 is inoculated in a PDA culture medium for culture.
5. A method for preparing the biocontrol microbial agent of any one of claims 2-4, comprising the steps of:
s1, mixing corn flour, rice straw and humic acid to prepare a matrix;
s2, adding compact basket bacteria into the matrixTalaromyces adpressus) Hyphae or spores of PK-1;
s3, adding sterile water to enable the water content of the mixture obtained in the step S2 to be 50% -60%;
and S4, sealing and culturing the product obtained in the step S3 for a plurality of days to obtain the biocontrol microbial agent.
6. A microbial pesticide, characterized in that an active ingredient of the microbial pesticide comprises the biocontrol bacterium of claim 1 or the biocontrol microbial agent of any one of claims 2 to 4.
7. Use of the biocontrol bacterium of claim 1, the biocontrol agent of any one of claims 2-4 or the microbial pesticide of claim 6 for controlling plant root rot, characterized in that the root rot comprises root rot caused by one or more fungi of the group consisting of phytophthora sojae, pythium ultimum, fusarium oxysporum, fusarium solani, fusarium equisetum, fusarium graminearum and phomopsis.
8. A method for controlling plant root rot, comprising applying the biocontrol bacterium according to claim 1, the biocontrol agent according to any one of claims 2 to 4 or the microbial pesticide according to claim 6 to a plant growth environment, wherein the root rot comprises a root rot caused by one or more fungi of phytophthora sojae, pythium ultimum, fusarium oxysporum, fusarium solani, fusarium equisetum, fusarium graminearum and Phomopsis.
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