CN116572525B - Biological 3D printer of integrated biological cultivation function - Google Patents
Biological 3D printer of integrated biological cultivation function Download PDFInfo
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- CN116572525B CN116572525B CN202310388721.3A CN202310388721A CN116572525B CN 116572525 B CN116572525 B CN 116572525B CN 202310388721 A CN202310388721 A CN 202310388721A CN 116572525 B CN116572525 B CN 116572525B
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- 238000004113 cell culture Methods 0.000 claims description 4
- 230000003287 optical effect Effects 0.000 claims description 4
- 238000010438 heat treatment Methods 0.000 claims description 3
- 230000001954 sterilising effect Effects 0.000 claims description 2
- 238000007639 printing Methods 0.000 abstract description 21
- 238000005516 engineering process Methods 0.000 abstract description 7
- 238000000034 method Methods 0.000 abstract description 7
- 230000008569 process Effects 0.000 abstract description 5
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- 238000010146 3D printing Methods 0.000 abstract description 2
- 238000012546 transfer Methods 0.000 abstract description 2
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- 238000001125 extrusion Methods 0.000 description 5
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- 238000003860 storage Methods 0.000 description 4
- 230000004083 survival effect Effects 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 3
- 239000012620 biological material Substances 0.000 description 2
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- 238000003854 Surface Print Methods 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
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Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
- B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
- B29C64/00—Additive manufacturing, i.e. manufacturing of three-dimensional [3D] objects by additive deposition, additive agglomeration or additive layering, e.g. by 3D printing, stereolithography or selective laser sintering
- B29C64/10—Processes of additive manufacturing
- B29C64/106—Processes of additive manufacturing using only liquids or viscous materials, e.g. depositing a continuous bead of viscous material
- B29C64/124—Processes of additive manufacturing using only liquids or viscous materials, e.g. depositing a continuous bead of viscous material using layers of liquid which are selectively solidified
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
- B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
- B29C64/00—Additive manufacturing, i.e. manufacturing of three-dimensional [3D] objects by additive deposition, additive agglomeration or additive layering, e.g. by 3D printing, stereolithography or selective laser sintering
- B29C64/20—Apparatus for additive manufacturing; Details thereof or accessories therefor
- B29C64/227—Driving means
- B29C64/236—Driving means for motion in a direction within the plane of a layer
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
- B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
- B29C64/00—Additive manufacturing, i.e. manufacturing of three-dimensional [3D] objects by additive deposition, additive agglomeration or additive layering, e.g. by 3D printing, stereolithography or selective laser sintering
- B29C64/20—Apparatus for additive manufacturing; Details thereof or accessories therefor
- B29C64/227—Driving means
- B29C64/241—Driving means for rotary motion
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
- B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
- B29C64/00—Additive manufacturing, i.e. manufacturing of three-dimensional [3D] objects by additive deposition, additive agglomeration or additive layering, e.g. by 3D printing, stereolithography or selective laser sintering
- B29C64/20—Apparatus for additive manufacturing; Details thereof or accessories therefor
- B29C64/245—Platforms or substrates
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
- B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
- B29C64/00—Additive manufacturing, i.e. manufacturing of three-dimensional [3D] objects by additive deposition, additive agglomeration or additive layering, e.g. by 3D printing, stereolithography or selective laser sintering
- B29C64/30—Auxiliary operations or equipment
- B29C64/379—Handling of additively manufactured objects, e.g. using robots
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B33—ADDITIVE MANUFACTURING TECHNOLOGY
- B33Y—ADDITIVE MANUFACTURING, i.e. MANUFACTURING OF THREE-DIMENSIONAL [3-D] OBJECTS BY ADDITIVE DEPOSITION, ADDITIVE AGGLOMERATION OR ADDITIVE LAYERING, e.g. BY 3-D PRINTING, STEREOLITHOGRAPHY OR SELECTIVE LASER SINTERING
- B33Y30/00—Apparatus for additive manufacturing; Details thereof or accessories therefor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B33—ADDITIVE MANUFACTURING TECHNOLOGY
- B33Y—ADDITIVE MANUFACTURING, i.e. MANUFACTURING OF THREE-DIMENSIONAL [3-D] OBJECTS BY ADDITIVE DEPOSITION, ADDITIVE AGGLOMERATION OR ADDITIVE LAYERING, e.g. BY 3-D PRINTING, STEREOLITHOGRAPHY OR SELECTIVE LASER SINTERING
- B33Y40/00—Auxiliary operations or equipment, e.g. for material handling
- B33Y40/20—Post-treatment, e.g. curing, coating or polishing
Abstract
The invention relates to the technical field of biological 3D printing, in particular to a biological 3D printer integrating a biological culture function. The invention provides a biological 3D printer integrating biological culture function based on DLP printing technology. The utility model provides a biological 3D printer of integrated biological cultivation function, includes organism, chamber door and DLP projection ray apparatus etc. the organism side is equipped with rotatable chamber door of opening, installs the DLP projection ray apparatus in the organism, and triaxial moving mechanism is installed at the organism top, and triaxial moving mechanism includes X axial translation unit, Y axial translation unit and Z axial translation unit, and the printer head is installed at Y axial translation unit, and the bio-ink groove is fixed on the organism, bio-ink groove is located the top of DLP projection ray apparatus. According to the invention, the cell solution tank and the biological culture chamber are integrated on the machine body, so that the formed biological tissue can be timely taken down and cultured, and the formed biological tissue can be effectively prevented from losing activity in the transfer process.
Description
Technical Field
The invention relates to the technical field of biological 3D printing, in particular to a biological 3D printer integrating a biological culture function.
Background
The 3D biological printer is equipment capable of positioning and assembling biological materials or cell units according to an additive manufacturing principle under the drive of a digital three-dimensional model to manufacture products such as medical equipment, tissue engineering scaffolds, tissue organs and the like. 3D bioprinting is more complex than non-bioprinting: technical challenges related to living cell sensitivity and tissue architecture, including selection of materials, cell type, growth and differentiation factors, are to be overcome. Solving these complex problems requires integrating technologies from the fields of engineering, biological material science, cell biology, physics and medicine. 3D bioprinting techniques have been used for the generation and transplantation of a variety of tissues including multilaminate skin, bone, vascular grafts, tracheal splints, cardiac tissue, and cartilage structures.
Patent publication number CN212352909U discloses a biological 3D printer in 2021, month 1, comprising: the bottom plate is connected in the printer shell through the first linear moving mechanism; the spray head is connected in the printer shell through a second linear moving mechanism and comprises a storage cylinder, the bottom of the storage cylinder is provided with a discharge hole, the discharge hole is opposite to the bottom plate, the spray head is provided with a steering mechanism for rotating the angle of the discharge hole, and the spray head is also provided with a compression bar which stretches into the storage cylinder to press printing materials in the storage cylinder to be extruded from the discharge hole; and the cutting mechanism is arranged between the discharge hole of the spray head and the bottom plate and is used for cutting the extruded printing material.
According to the printer, the discharge hole is arranged into the plurality of strip-shaped holes, one-time extrusion can be performed to form a layer, and the purpose of improving the printing efficiency is achieved, but the mode is unfavorable for cell survival, cells in fluid are easy to lose activity under the action of shearing stress, and the maintenance of high survival rate is the key for realizing the tissue function.
Disclosure of Invention
In order to overcome the defect that when the existing biological 3D printer adopts extrusion type biological printing, cells in fluid are easy to lose activity under the action of shearing stress, so that the initial tissue formed by printing is difficult to cultivate to realize tissue functions, in addition, the formed initial tissue needs to be transferred from the printer to a cultivation device, and the cell survival rate can be further reduced in the transfer process, the invention provides the biological 3D printer based on the DLP printing technology and integrating the biological cultivation function.
The utility model provides a biological 3D printer of integrated biological cultivation function, includes organism, chamber door, DLP projection ray apparatus, triaxial moving mechanism, print head and biological ink basin, and the organism side is equipped with rotatable chamber door of opening, installs DLP projection ray apparatus in the organism, and triaxial moving mechanism is installed at the organism top, and triaxial moving mechanism includes X axial translation unit, Y axial translation unit and Z axial translation unit, and the print head is installed at Y axial translation unit, and biological ink basin is fixed on the organism, biological ink basin is located the top of DLP projection ray apparatus to it has biological ink to fill, but biological ink basin bottom surface printing opacity still including the guide post, place board and cell solution groove, install the guide post in the organism, install liftable place the board on the guide post, the cell solution groove is placed on placing the board, and its intussuseption is filled with cell culture solution.
Optionally, still including baffle and cultivate the room, be equipped with vertical baffle in the organism, the baffle separates into two preceding cavities around with organism inner space, and DLP projection ray apparatus is located preceding cavity, and back cavity is cultivateed the room, cultivate room including environmental conditioning module, diaphragm and sliding plate, cultivate indoor being provided with environmental conditioning module, environmental conditioning module is including at least the heating lamp that is used for temperature regulation and the ultraviolet lamp that is used for disinfecting, cultivates indoor two piece at least diaphragms of installing, all is equipped with the sliding plate that can follow the chamber door sideslip on the diaphragm.
Optionally, the partition plate is provided with openings with bottom edges being flush with the top surface of the sliding plate, and the number of the openings is the same as that of the transverse plates.
Optionally, the lifting assembly comprises an electric push rod and a connecting frame, the electric push rod is arranged in the cavity in front of the machine body, the connecting frame is sleeved on the electric push rod, the top end of the connecting frame is fixedly connected with the placing plate, and when the electric push rod moves in the vertical direction, the connecting frame is driven to move up and down together with the placing plate.
Optionally, the device also comprises a limiting rod, the limiting rod is slidably installed at the front side of the machine body, the limiting rod penetrates through the front wall of the machine body and stretches into the front cavity, and the number and the height of the limiting rod correspond to those of the transverse plates.
Optionally, the cell solution tank comprises a push plate, wherein a chute is formed in the middle of the placing plate, the push plate is connected to the placing plate in a sliding manner, the push plate can horizontally move along the chute, and the cell solution tank can move under the action of the push plate.
Optionally, still including automatic push-in subassembly, automatic push-in subassembly is including rotary rod, guide block, connecting rod, framework and horizontal pole, place the board bottom and install the rotary rod, the rotary rod can be followed its one end that is close to electric putter and rotated, it has logical groove to open on the rotary rod, the push pedal bottom stretches into the logical inslot of rotary rod, can promote the push pedal to remove along the spout when the rotary rod rotates, guide block fixed mounting is placing the board downside, the connecting rod passes the guide block and stretches into the logical inslot of rotary rod, and can follow the guide block back-and-forth movement and promote the rotary rod to rotate, the connecting rod bottom extends to electric putter side, its terminal fixedly connected with framework, the framework bottom contacts with the link, the framework hollow part is the parallelogram that low back was high in front, electric putter top fixed mounting has the horizontal pole, the horizontal pole stretches into in the framework, when the horizontal pole moves down relatively, the extrusion framework hypotenuse drives the connecting rod and slides backward.
Optionally, still including telescopic link, baffle and elastic component, install the telescopic link in the cavity before the organism, telescopic link top is connected with the baffle, the baffle is laminated with the baffle, and the baffle can slide from top to bottom relative to the baffle, covers the opening when rising and seals, places the board and pushes down the baffle when moving down, be connected with the elastic component between the fixed part of telescopic link and the telescopic part.
Compared with the prior art, the invention has the following advantages: 1. the invention adopts the DLP printing technology to carry out biological printing, and based on the layer scanning forming principle of the digital surface light source, the printing speed of the DLP technology is faster, and the printing precision is also very high, compared with the biological printing based on extrusion, the DLP biological printing can effectively ensure the cell survival rate and shorten the biological manufacturing time;
2. according to the invention, the cell solution tank and the biological culture chamber are integrated on the machine body, so that the formed biological tissue can be timely taken down and cultured, the process of transferring the tissue from the printer to the biological culture equipment is omitted, the formed biological tissue can be effectively prevented from losing activity in the transferring process, and the printing efficiency is improved;
3. according to the invention, through ingenious matching between the lifting component and the pushing component, the cell solution tank filled with the forming group fabric can be transferred into the culture room in an electric control mode, so that risks caused by manual operation errors are avoided.
Drawings
Fig. 1 is a schematic perspective view of the present invention.
Fig. 2 is a schematic perspective view of the inside of the body of the present invention.
FIG. 3 is a schematic perspective view showing the structure of the guide column, the placement plate and the cell solution tank according to the present invention.
FIG. 4 is a schematic perspective view of a culture chamber according to the present invention.
Fig. 5 is a schematic perspective view of a lifting assembly according to the present invention.
Fig. 6 is a schematic perspective view of a stop lever according to the present invention.
Fig. 7 is a schematic perspective view of a push plate according to the present invention.
Fig. 8 is a schematic perspective view of the bottom of the placement plate according to the present invention.
Fig. 9 is an enlarged view of a portion of the push-in assembly of the present invention.
Fig. 10 is a schematic perspective view of the push-in assembly of the present invention.
Fig. 11 is a schematic perspective view of the telescopic rod and the baffle of the present invention.
Fig. 12 is a schematic perspective view of an elastic member according to the present invention.
The reference symbols in the drawings: 1: organism, 2: door, 3: DLP projection ray apparatus, 4: triaxial moving mechanism, 5: printhead, 6: bio-ink tank, 7: guide post, 8: placing plate, 81: chute, 9: cell solution tank, 10: baffle, 101: opening, 11: culture chamber, 111: environmental conditioning module, 112: cross plate, 113: slide plate, 12: lifting assembly, 121: electric putter, 122: connecting frame, 13: limit lever, 14: push plate, 15: push assembly, 151: rotating rod, 152: through slot, 153: guide block, 154: connecting rod, 155: frame body, 156: cross bar, 16: telescoping rod, 17: baffle, 18: an elastic member.
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings, for the purpose of making the objects, technical solutions and advantages of the present invention more apparent. It is only stated that the terms of orientation such as up, down, left, right, front, back, inner, outer, etc. used in this document or the imminent present invention, are used only with reference to the drawings of the present invention, and are not meant to be limiting in any way.
Example 1
1-3D printer of integrated biological cultivation function, as shown in fig. 3, including organism 1, chamber door 2, DLP projection ray apparatus 3, triaxial moving mechanism 4, print head 5 and biological ink basin 6, organism 1 side articulates there is rotatable chamber door 2 of opening, be equipped with the handle of being convenient for grip on the chamber door 2, install DLP projection ray apparatus 3 through the bolt in the organism 1, DLP projection ray apparatus 3 mainly comprises DLP projection equipment, cloud platform and speculum, organism 1 top is through the bolt installation triaxial moving mechanism 4, triaxial moving mechanism 4 includes X axial translation unit, Y axial translation unit and Z axial translation unit, print head 5 installs at Y axial translation unit, print head 5 can carry out arbitrary removal in the XOZ plane, and carry out the regulation of front and back position along the Y axial, biological ink basin 6 is fixed at organism 1 top through the mode of magnetic adsorption, biological ink basin 6 is located DLP projection ray apparatus 3's top, and is filled with the biological ink that photosensitive material based on DLP technique makes, biological ink basin 6 light-permeable, still include guide post 7, place guide post 8 and 9 and place the guide post 7 are equipped with in the cell culture solution groove 7, install two guide post 7 and place the guide post 7 and are equipped with the cell solution groove 7 in the guide post is placed on it, and the guide post 8 is placed in the cell is placed on the guide post 7.
As shown in fig. 4, the device further comprises a partition board 10 and a culture chamber 11, wherein a vertical partition board 10 is welded in the machine body 1, the partition board 10 divides the inner space of the machine body 1 into a front chamber and a rear chamber, the DLP projection optical machine 3 is positioned in the front chamber, the rear chamber is the culture chamber 11, the culture chamber 11 comprises an environment adjusting module 111, a transverse board 112 and a sliding board 113, the environment adjusting module 111 is arranged in the culture chamber 11, the environment adjusting module 111 comprises a heating lamp for adjusting temperature, an ultraviolet lamp for sterilizing and a humidity controller for controlling humidity, two transverse boards 112 are arranged in the culture chamber 11 through screws, the interval is about 1.5 times of the height of the cell solution tank 9, the sliding boards 113 which can slide out from the box door 2 are connected on the transverse boards 112 in a sliding manner, grooves which are convenient to grasp are formed on the side of the sliding boards 113, which are close to the box door 2, openings 101 with the bottom edges which are flush with the top surfaces of the sliding boards 113 are arranged in the space between the height of the cell solution tank 9 and the transverse boards 112, and the number of the openings 101 is two.
When biological printing is needed, the liquid biological substrate based on the DLP technology is firstly added into the biological ink tank 6, then the triaxial moving mechanism 4 is controlled to adjust the printing head 5 to a proper position, the DLP projection optical machine 3 is driven to emit visible light, the required pattern is selectively reflected through the reflecting mirror, the cross-section image of the printing layer is projected onto one layer of the biological substrate in the biological ink tank 6, the material is quickly solidified, then the triaxial moving mechanism 4 is used for controlling the printing head 5 to slowly and vertically move a layer distance from the biological ink tank 6, printing is carried out layer by layer, the DLP photocuring printing technology is adopted, stress caused by extrusion can be effectively avoided, after printing is finished, the formed initial group fabric is taken down and quickly put into the cell solution tank 9 beside, and the formed initial group fabric is driven to drop into the front side cavity of the machine body 1 by sliding down the placing plate 8 along the guiding post 7, then the cross-section image is sent into the culture chamber 11 through the opening 101 of the partition board 10, the environment adjusting module 111 in the culture chamber 11 can adjust the temperature in the culture chamber 11, the temperature of the initial group fabric can be kept at a certain distance from the biological ink tank 6, the cell viability is effectively prevented from being influenced by the ultraviolet light, and the ultraviolet light can be effectively pulled out from the ultraviolet light plate 112 when the ultraviolet light is required to be prevented from being turned on the ultraviolet light plate 112.
Example 2
On the basis of embodiment 1, as shown in fig. 5, the lifting assembly 12 for controlling the placing plate 8 to move vertically is further included, the lifting assembly 12 comprises an electric push rod 121 and a connecting frame 122, the electric push rod 121 is installed in a front cavity of the machine body 1 through bolts, the electric push rod 121 can move linearly in the vertical direction, the connecting frame 122 is sleeved on a movable part on the upper side of the electric push rod 121, the top end of the connecting frame 122 is fixedly connected with the ground of the placing plate 8 through bolts, the appearance of the connecting frame 122 is similar to that of an 'B' -shaped structure, two ends of the connecting frame are horizontal transverse plates, and when the electric push rod 121 moves in the vertical direction, the connecting frame 122 and the placing plate 8 are driven to move up and down together.
As shown in fig. 6, the device further comprises a limiting rod 13 for limiting the downward movement of the placing plate 8, the front side of the machine body 1 is slidably provided with the limiting rod 13, the limiting rod 13 can slide back and forth relative to the machine body 1 so as to push in to realize limiting or withdraw from to release limiting, the limiting rod 13 penetrates through the front wall of the machine body 1 and stretches into the front cavity, two limiting rods are arranged in total, the limiting rod 13 consists of a rod part vertical to the front wall of the machine body 1 and a holding part vertical to the outer end of the rod part, and the holding part is vertical to the rod part.
When the placing plate 8 needs to be controlled to drive the cell solution tank 9 and the initial group fabric therein to move into the machine body 1, the electric push rod 121 is driven to move downwards in the vertical direction, the movable part of the electric push rod drives the connecting frame 122 and the placing plate 8 to descend together, and when the electric push rod is lowered to the position of the opening 101, the inwardly extending limiting rod 13 organizes the placing plate 8 to continue to fall, at the moment, the cell solution tank 9 can be pushed into the culture chamber 11, and then the electric push rod 121 is controlled to drive the placing plate 8 to rise again, so that the interior of the machine body 1 is kept closed.
Example 3
On the basis of embodiment 2, as shown in fig. 7 and 8, the cell solution tank 9 further comprises a push plate 14 for pushing the object on the surface of the placing plate 8, wherein the push plate 14 is a T-shaped plate, a sliding groove 81 is formed in the middle of the placing plate 8, the push plate 14 is slidably connected to the placing plate 8, the push plate 14 can horizontally move along the sliding groove 81, and the cell solution tank 9 can move to the rear side under the action of the push plate 14.
As shown in fig. 9 and 10, the automatic pushing assembly 15 for automatically pushing the cell solution tank 9 into the culture chamber 11 is further included, the automatic pushing assembly 15 includes a rotating rod 151, a guide block 153, a connecting rod 154, a frame 155 and a cross rod 156, the bottom of the placing plate 8 is hinged with the rotating rod 151, the rotating rod 151 can rotate along one end of the rotating rod 151, which is close to the electric push rod 121, as a center point, the rotating rod 151 is perpendicular to the connecting frame 122 in an initial state, a through groove 152 is formed in the middle of the rotating rod 151, the bottom end of the push plate 14 extends into the through groove 152 of the rotating rod 151, the push plate 14 can be pushed to move along the sliding groove 81 when the rotating rod 151 rotates, the guide block 153 is fixedly installed on the bottom side of the placing plate 8 and parallel to the sliding groove 81 through a cylindrical connecting block 153 extending downwards, the connecting rod 154 penetrates through the guide block 153 into the through groove 152 of the rotating rod 151, and can move the rotating rod 151 forwards and backwards along the guide block 153, the connecting rod 154 is an L-shaped rod, the bottom end of the connecting rod 154 extends towards the electric push rod 121, the end of the frame 155 is welded with the frame 155, the bottom of the frame 155 contacts the connecting frame 122, the frame 155 is in a trapezoid shape, the outer edge of the frame 155 is perpendicular to the lower than the frame 155, the lower end of the frame 155 is perpendicular to the upper side of the frame 155, and the lower side of the frame 155 is driven to the lower side of the frame 155 is welded to the lower than the lower side of the frame 155, and the lower side of the frame 155 is perpendicular to the lower side of the frame 155 is welded.
When the placing plate 8 moves to be flush with the sliding plate 113, the placing plate 8 is limited by the limiting rod 13 and does not slide down any more, at this time, the movable part of the electric push rod 121 continues to move downwards, and moves downwards relative to the connecting frame 122, the cross rod 156 at the top of the connecting frame 122 synchronously moves downwards, slides down to the bevel edge of the bottom along the rear side of the frame 155, and is blocked by the limiting rod 13 together with the placing plate 8 due to the contact of the bottom of the frame 155 with the connecting frame 122, so that the bevel edge of the bottom side of the frame 155 is extruded, the frame 155 moves backwards, the connecting rod 154 moves backwards along the guide block 153 to push the rotary rod 151 to rotate anticlockwise, the rotary rod 151 pushes the push plate 14 to move backwards along the sliding groove 81, so that the cell solution tank 9 at the upper side of the placing plate 8 moves backwards, and pushes the cell solution tank 9 on the baffle 17 to the sliding plate 113 through the opening 101 on the baffle 17, and each component moves upwards and resets along with the movable part of the electric push rod 121 due to the same bevel edge of the frame 155.
As shown in fig. 11 and 12, the telescopic rod 16, the baffle 17 and the elastic element 18 are further included, the telescopic rod 16 is installed in the front cavity of the machine body 1 through bolts, the top of the telescopic rod 16 is connected with the baffle 17 in a manner of bonding through strong fixing glue, the baffle 17 is attached to the partition 10, the baffle 17 can slide up and down relative to the partition 10, the opening 101 is covered and sealed when the baffle 17 is lifted, the periphery of the baffle 17 extends to the front side, the baffle 17 is pressed down when the placing plate 8 moves downwards, the elastic element 18 is connected between the fixing part and the telescopic part of the telescopic rod 16, and the elastic element 18 adopts a stainless steel compression spring.
In the process that the cell solution tank 9 descends from the top of the machine body 1 to enter the culture chamber 11, the placing plate 8 moves downwards to press the baffle 17, the telescopic part of the telescopic rod 16 moves downwards under the stress of the elastic piece 18 to compress, the baffle 17 moves away from the opening 101, the cell solution tank 9 is convenient to enter the culture chamber 11, when the placing plate 8 is lifted, the telescopic part of the telescopic rod 16 extends upwards under the reset action of the elastic piece 18 to push the baffle 17 to move upwards, and the opening 101 on the partition plate 10 is covered and sealed again.
The foregoing description of the preferred embodiments of the invention is not intended to limit the invention to the precise form disclosed, and any such modifications, equivalents, and alternatives falling within the spirit and scope of the invention are intended to be included within the scope of the invention.
Claims (2)
1. The utility model provides a biological 3D printer of integrated biological cultivation function, including organism (1), chamber door (2), DLP projection ray apparatus (3), triaxial moving mechanism (4), print head (5) and biological ink basin (6), organism (1) side is equipped with rotatable chamber door (2) of opening, install DLP projection ray apparatus (3) in organism (1), triaxial moving mechanism (4) are installed at organism (1) top, triaxial moving mechanism (4) include X axial translation unit, Y axial translation unit and Z axial translation unit, print head (5) are installed at Y axial translation unit, biological ink basin (6) are fixed on organism (1), biological ink basin (6) are located the top of DLP projection ray apparatus (3) to it has biological ink to fill, biological ink basin (6) bottom surface light-permeable;
the cell culture device is characterized by further comprising a guide column (7), a placing plate (8) and a cell solution tank (9), wherein the guide column (7) is installed in the machine body (1), the placing plate (8) capable of lifting is installed on the guide column (7), and the cell solution tank (9) is placed on the placing plate (8) and is filled with cell culture solution;
the DLP projection optical machine is characterized by further comprising a partition plate (10) and a culture chamber (11), wherein the vertical partition plate (10) is arranged in the machine body (1), the partition plate (10) divides the inner space of the machine body (1) into a front chamber and a rear chamber, the DLP projection optical machine (3) is positioned in the front chamber, the rear chamber is the culture chamber (11), the culture chamber (11) comprises an environment adjusting module (111), a transverse plate (112) and a sliding plate (113), the environment adjusting module (111) is arranged in the culture chamber (11), the environment adjusting module (111) at least comprises a heating lamp for adjusting the temperature and an ultraviolet lamp for sterilizing, at least two transverse plates (112) are arranged in the culture chamber (11), and the sliding plate (113) capable of sliding out from a box door (2) is arranged on each transverse plate (112);
the partition board (10) is provided with openings (101) with the bottom edges being flush with the top surface of the sliding board (113), and the number of the openings (101) is the same as that of the transverse boards (112);
the lifting assembly (12) comprises an electric push rod (121) and a connecting frame (122), the electric push rod (121) is arranged in a front cavity of the machine body (1), the connecting frame (122) is sleeved on the electric push rod (121), the top end of the connecting frame (122) is fixedly connected with the placing plate (8), and when the electric push rod (121) moves in the vertical direction, the connecting frame (122) and the placing plate (8) are driven to do lifting motion together;
the device also comprises a limiting rod (13), wherein the limiting rod (13) is slidably arranged at the front side of the machine body (1), the limiting rod (13) penetrates through the front wall of the machine body (1) to extend into the front cavity, and the number and the height of the limiting rod are corresponding to those of the transverse plates (112);
the cell solution tank (9) can move under the action of the push plate (14);
the automatic pushing assembly (15) comprises a rotating rod (151), a guide block (153), a connecting rod (154), a frame body (155) and a cross rod (156), wherein the rotating rod (151) is installed at the bottom of the placing plate (8), the rotating rod (151) can rotate along one end, close to the electric push rod (121), of the rotating rod (151), a through groove (152) is formed in the rotating rod (151), the bottom end of the push plate (14) stretches into the through groove (152) of the rotating rod (151), the push plate (14) can be pushed to move along the sliding groove (81) when the rotating rod (151) rotates, the guide block (153) is fixedly installed at the bottom side of the placing plate (8), the connecting rod (154) penetrates through the guide block (153) to stretch into the through groove (152) of the rotating rod (151), the bottom end of the connecting rod (154) extends towards the side of the electric push rod (121), the end of the connecting rod is fixedly connected with the frame body (155), the bottom of the frame body (155) is contacted with the connecting frame (122), the hollow part of the frame body (155) is in a front low shape, the upper side and lower side of the connecting rod (155) is fixedly installed at the top end of the connecting rod (155), the upper side and lower side of the connecting rod (155) of the connecting rod is fixedly installed at the upper side and lower side of the connecting rod (155), when the placing plate (8) moves to be flush with the sliding plate (113), the placing plate (8) is limited by the limiting rod (13) and does not slide down any more, at the moment, the movable part of the electric push rod (121) continues to move downwards, the movable part moves downwards relative to the connecting frame (122), the cross rod (156) at the top of the connecting frame (122) moves downwards synchronously, and when the cross rod (156) moves downwards relative to the frame (155), the inclined edge of the frame (155) is extruded to drive the connecting rod (154) to slide backwards.
2. The biological 3D printer integrating biological culture function according to claim 1, further comprising a telescopic rod (16), a baffle plate (17) and an elastic piece (18), wherein the telescopic rod (16) is arranged in a front cavity of the machine body (1), the top of the telescopic rod (16) is connected with the baffle plate (17), the baffle plate (17) is attached to the baffle plate (10), the baffle plate (17) can slide up and down relative to the baffle plate (10), the opening (101) is covered and sealed when the baffle plate (17) is lifted, the baffle plate (17) is pressed down when the placing plate (8) moves downwards, and the elastic piece (18) is connected between a fixing part and the telescopic part of the telescopic rod (16).
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| CN202310388721.3A CN116572525B (en) | 2023-04-13 | 2023-04-13 | Biological 3D printer of integrated biological cultivation function |
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| CN116572525A (en) | 2023-08-11 |
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