CN116559125A - Large-flow biological aerosol intrinsic fluorescence monitoring device - Google Patents
Large-flow biological aerosol intrinsic fluorescence monitoring device Download PDFInfo
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- CN116559125A CN116559125A CN202210105446.5A CN202210105446A CN116559125A CN 116559125 A CN116559125 A CN 116559125A CN 202210105446 A CN202210105446 A CN 202210105446A CN 116559125 A CN116559125 A CN 116559125A
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- intrinsic fluorescence
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- 239000000443 aerosol Substances 0.000 title claims abstract description 39
- 238000012806 monitoring device Methods 0.000 title claims abstract description 14
- 239000002245 particle Substances 0.000 claims abstract description 97
- 239000007788 liquid Substances 0.000 claims abstract description 94
- 238000005070 sampling Methods 0.000 claims abstract description 93
- 230000002572 peristaltic effect Effects 0.000 claims abstract description 25
- 238000001917 fluorescence detection Methods 0.000 claims abstract description 24
- 238000001514 detection method Methods 0.000 claims abstract description 22
- 238000012544 monitoring process Methods 0.000 claims abstract description 15
- 238000001914 filtration Methods 0.000 claims abstract description 11
- 239000007791 liquid phase Substances 0.000 claims abstract description 8
- 230000003287 optical effect Effects 0.000 claims description 7
- 244000005700 microbiome Species 0.000 claims description 6
- 238000007493 shaping process Methods 0.000 claims description 3
- 238000005516 engineering process Methods 0.000 abstract description 11
- 230000000694 effects Effects 0.000 description 4
- 230000002238 attenuated effect Effects 0.000 description 2
- 238000004061 bleaching Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 235000019504 cigarettes Nutrition 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/22—Devices for withdrawing samples in the gaseous state
- G01N1/24—Suction devices
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/20—Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters
Abstract
The device comprises a large-flow sampling fan, a sampling cup filled with liquid phase medium, a particle counter arranged at an air inlet end, a fluorescence detection unit, a particle filtering unit, a liquid outlet peristaltic pump, a liquid storage unit, a liquid inlet peristaltic pump and a central control unit; concentrating a bioaerosol particle sample by a high-flow liquid phase sampling technology, and monitoring the intrinsic fluorescence of the bioaerosol particles by an ultraviolet-induced intrinsic fluorescence technology to realize high-sensitivity and rapid detection of the low-concentration bioaerosol particles; the closed loop liquid path system of the particle filter is adopted, so that the sampling liquid can be reused for a plurality of times, and the long-time automatic monitoring of the monitoring device is realized. The invention can detect and obtain multiple information such as particle concentration, intrinsic fluorescence and bleached intrinsic fluorescence of the enriched biological aerosol particle sample, and can realize the detection of low false alarm rate and the primary classification of aerosol particles.
Description
Technical Field
The invention relates to a biological aerosol optical real-time monitoring technology, in particular to a high-flow biological aerosol intrinsic fluorescence monitoring device.
Background
Bioaerosols are commonly found in air environments and are typically low in concentration, with bacteria and other microorganisms in the air being present in concentrations of about several hundred per cubic meter. In the current real-time monitoring technology of the biological aerosol, the ultraviolet excitation light induced intrinsic fluorescence detection technology has the advantages of strong real-time performance, high automation degree, no consumable and the like, and has better application effect in partial scenes.
However, the conventional intrinsic fluorescence monitoring sensor generally has a sampling flow rate in a range of 1-5L/min, has a good practical effect on early warning of high-concentration biological aerosol after release of a bioterrorism agent, and has a poor response effect on low-concentration biological aerosol under the conventional condition. In order to meet the requirement of large-flow sampling, a virtual impact concentrator is additionally arranged in front of the fluorescence detector, the virtual impact concentrator can enrich aerosol particles with more than the cutting particle size in the large-flow air flow into the small-flow air flow, but the cutting particle size of the virtual impact concentrator is generally larger and cannot cover the aerosol particles with submicron particle size, so that the fluorescence detection device additionally provided with the virtual impact device can only detect the biological aerosol particles with larger particle size.
On the other hand, the conventional bioaerosol fluorescence detection device is affected by an interfering object such as haze or cigarettes, and sometimes gives a false alarm.
Disclosure of Invention
Aiming at the existing problems, the invention provides a large-flow bioaerosol intrinsic fluorescence monitoring device, which is characterized in that a large-flow liquid phase sampling technology is used for concentrating a bioaerosol particle sample, and an ultraviolet induced intrinsic fluorescence technology is used for monitoring the intrinsic fluorescence of the bioaerosol particles, so that the high-sensitivity and rapid detection of low-concentration bioaerosol particles is realized; the closed loop liquid path system of the particle filter is adopted, so that the sampling liquid can be reused for a plurality of times, and the long-time automatic monitoring of the monitoring device is realized. The device can detect and obtain multiple information such as particle concentration, intrinsic fluorescence and bleached intrinsic fluorescence of the enriched biological aerosol particle sample, and accordingly, the detection of the low false alarm rate and the primary classification of aerosol particles can be realized.
The technical scheme of the invention is as follows:
the device is characterized by comprising a large-flow sampling fan, a sampling cup filled with liquid phase medium, a particle counter arranged at an air inlet end, a fluorescence detection unit, a particle filtering unit, a liquid outlet peristaltic pump, a liquid storage unit, a liquid inlet peristaltic pump and a central control unit,
the sampling cup is integrally formed by an upper section cylinder and a lower section cone cup shell-shaped structure, the cone cup shell-shaped structure is used for containing sampling liquid, the cylindrical upper end of the sampling cup is communicated with the high-flow sampling fan to form a cyclone sampling device, an airflow connecting pipe is arranged at a lower position of one side surface of the cylindrical upper section of the sampling cup, one side of the airflow connecting pipe is connected with the particle counter, a liquid inlet is arranged at a higher position of the other side surface of the cylindrical upper section of the sampling cup, an ultraviolet and visible light antireflection film is plated on the surface of the cone cup shell of the sampling cup, the cone tip end of the cone cup shell of the sampling cup is communicated with the liquid storage unit through a particle filtering unit and a liquid outlet peristaltic pump, and the other pipeline of the liquid storage unit outlet is connected with the liquid inlet of the sampling cup through the liquid inlet peristaltic pump;
the fluorescent detection unit comprises an ultraviolet light emitting diode, a shaping optical component, a detection area of the cone-shaped shell of the sampling cup and a light trap are sequentially arranged along the laser output direction of the ultraviolet light emitting diode, aerosol particles in the detection area emit intrinsic fluorescence under the irradiation of laser of the ultraviolet light emitting diode, the intrinsic fluorescence is collected by the focusing lens group and then parallelly enters the dichroic mirror, short-wave fluorescence of the intrinsic fluorescence is reflected by the dichroic mirror and then sequentially passes through the short-wave filter, the short-wave focusing lens and the first diaphragm and then reaches the first fluorescent detector, and long-wave fluorescence of the intrinsic fluorescence is transmitted through the dichroic mirror and then sequentially passes through the long-wave filter, the long-wave focusing lens and the second diaphragm and then reaches the second fluorescent detector.
The detection area (namely the light sensitive area) of the fluorescence detection unit is positioned at the lower half part of the sampling liquid of the sampling cup.
The central control unit can control the start and stop of the sampling fan, the particle counter, the fluorescence detection unit, the liquid outlet peristaltic pump and the liquid inlet peristaltic pump, and can also receive detection results from the particle counter and the fluorescence detection unit.
The particle filter unit is a high-efficiency filter suitable for liquid, has a filter particle diameter not less than 0.22 mu m, can filter most of microorganism particles, and has a filter efficiency not less than 99.9%.
The working process of the large-flow biological aerosol monitoring device is as follows:
1) The liquid inlet peristaltic pump pumps about 10mL of sampling liquid medium from the liquid storage unit into the sampling cup, sampling liquid in the sampling cup forms a wet wall cyclone, and after the liquid inlet medium is finished, the fluorescence detection unit starts to work to excite the sampling liquid to obtain an optical background signal;
2) The large-flow fan and the particle counter start to work, after the large-flow fan works, air flows through the air flow connecting pipe and enters the sampling cup from the air inlet, a small part of air flows enter the particle counter and are used for measuring particle concentration to obtain a particle concentration value, most of air flows enter the sampling cup and are mixed with the liquid medium, aerosol particles are impacted and mixed into sampling liquid on the wall of the cup, the aerosol particles are collected into the liquid medium and are thus sampled and enriched, and the air flows are kept stand for more than 10 seconds after sampling is finished, and at the moment, most of particles including microorganisms are settled to the lower part of the sampling liquid;
3) The ultraviolet light emitting diode of the fluorescence detection unit starts to work, the emitted laser excites aerosol particles in the sampling liquid, the first fluorescence detector and the second fluorescence detector respectively obtain a first channel intrinsic fluorescence signal and a second channel intrinsic fluorescence signal, after continuous irradiation for a plurality of seconds, the intrinsic fluorescence intensity can be attenuated to generate a bleaching phenomenon, the attenuation speeds of different biological aerosol particles are different, and the first fluorescence detector and the second fluorescence detector respectively obtain a bleached first fluorescence signal and a bleached second fluorescence signal;
4) After fluorescence detection is finished, the liquid outlet peristaltic pump starts to work, the sampling liquid passes through the particle filtering unit under the drive of the liquid outlet peristaltic pump, aerosol particles in the sampling liquid can be intercepted by the particle filtering unit, purer sampling liquid returns to the liquid storage unit, and the sampling liquid is reserved for a subsequent period to be used as a new sampling liquid;
5) The particle concentration value and the fluorescence information are sent to the central control unit, so that the central control unit judges the biological attribute and the category of the aerosol particles according to the collected particle concentration value and fluorescence information.
Compared with the prior art, the invention has the following technical effects:
1. concentrating a bioaerosol particle sample by a high-flow liquid phase sampling technology, and monitoring the intrinsic fluorescence of the bioaerosol particles by an ultraviolet induced intrinsic fluorescence technology, so that high-sensitivity and rapid detection of the bioaerosol particles with low concentration can be realized;
2. the closed loop liquid path system comprising the particle filter can enable the sampling liquid to be reused for a plurality of times, thereby realizing long-time automatic monitoring of the monitoring device.
3. The device can detect and obtain multiple information such as particle concentration, intrinsic fluorescence and bleached intrinsic fluorescence of the enriched biological aerosol particle sample, and accordingly, the detection of the low false alarm rate and the primary classification of aerosol particles can be realized.
Drawings
Fig. 1 is a schematic diagram of the composition and working principle of the bioaerosol monitoring apparatus of the present invention.
Fig. 2 is a schematic diagram of the composition and principle of a fluorescence detection unit of the bioaerosol monitoring apparatus according to the present invention.
Detailed Description
The invention is further illustrated in the following figures and examples, which should not be taken to limit the scope of the invention.
As shown in figure 1, the high-flow biological aerosol intrinsic fluorescence monitoring device comprises a high-flow sampling fan 1, a sampling cup 2 filled with liquid phase medium, a particle counter 3 arranged at an air inlet end, a fluorescence detection unit 4, a particle filtering unit 5, a liquid outlet peristaltic pump 6, a liquid storage unit 7, a liquid inlet peristaltic pump 8 and a central control unit 9.
The sampling cup 2 is integrally formed by an upper section cylinder and a lower section cone cup shell-shaped structure, the cone cup shell-shaped structure is used for containing sampling liquid, the cylindrical upper end of the sampling cup 2 is communicated with the high-flow sampling fan 1 to form a cyclone sampling device, an airflow connecting pipe 21 is arranged at a lower side surface of the cylindrical upper section of the sampling cup 2, one side of the airflow connecting pipe 21 is connected with the particle counter 3, a liquid inlet 22 is arranged at a higher side surface of the cylindrical upper section of the sampling cup 2, an ultraviolet visible light antireflection film is plated on the surface of the cone cup shell of the sampling cup 2, the cone tip end of the cone cup shell of the sampling cup 2 is communicated with the liquid storage unit 7 through a pipeline through the particle filtering unit 5 and the liquid outlet peristaltic pump 6, and the other pipeline at the outlet of the liquid storage unit 7 is connected with the liquid inlet 22 of the sampling cup 2 through the liquid inlet peristaltic pump 8;
as shown in fig. 2, the fluorescence detection unit 4 includes an ultraviolet light emitting diode 401, a shaping optical component 402, a detection area of a cone-shaped shell of the sampling cup 2 and an optical trap 403 are sequentially arranged along the laser output direction of the ultraviolet light emitting diode 401, and aerosol particles in the detection area emit intrinsic fluorescence under the irradiation of laser light of the ultraviolet light emitting diode 401, the intrinsic fluorescence is collected by a focusing lens group 404 and then is parallel incident to a dichroic mirror 405, short-wave-band fluorescence of the intrinsic fluorescence is reflected by the dichroic mirror 405 and then sequentially passes through a short-wave filter 410, a short-wave focusing lens 411 and a first diaphragm 412 to reach a first fluorescence detector 413, and the long-wave-band fluorescence of the intrinsic fluorescence is transmitted through the dichroic mirror 405 and then sequentially passes through a long-wave filter 406, the long-wave focusing lens 407 and a second diaphragm 408 to reach a second fluorescence detector 409.
The detection area (i.e. the light sensitive area) of the fluorescence detection unit 4 is located at the lower half of the sample liquid of the sampling cup 2.
The high-flow biological aerosol intrinsic fluorescence monitoring device is characterized in that the central control unit 9 can control the start and stop of the sampling fan 1, the particle counter 3, the fluorescence detection unit 4, the liquid outlet peristaltic pump 6 and the liquid inlet peristaltic pump 8, and can also receive detection results from the particle counter 3 and the fluorescence detection unit 4.
The particle filter unit 5 is a high-efficiency filter suitable for liquid, has a filter particle diameter not less than 0.22 μm, can filter most of microorganism particles, and has a filter efficiency not less than 99.9%.
The working process of the large-flow biological aerosol monitoring device is as follows:
1) The peristaltic pump 8 pumps about 10mL of sampling liquid medium from the liquid storage unit 7 into the sampling cup 2, the sampling liquid in the sampling cup 2 forms a wet wall cyclone, and after the liquid inlet medium is finished, the fluorescent detection unit 4 starts to work to excite the sampling liquid to obtain an optical background signal;
2) The high-flow fan 2 and the particle counter 3 start to work, after the high-flow fan 2 works, air flows through the air flow connecting pipe 21 and enters the sampling cup 2 from the air inlet 22, a small part of air flows enter the particle counter 3 and are used for measuring the particle concentration to obtain a particle concentration value, most of air flows enter the sampling cup 2 and are mixed with the liquid medium, aerosol particles are impacted and mixed into the sampling liquid of the cup wall, the aerosol particles are collected into the liquid medium and are sampled and enriched, and after the sampling is finished, the air flows are kept stand for 10 seconds, and most of particles including microorganisms settle to the lower part of the sampling liquid;
3) The ultraviolet light emitting diode 401 of the fluorescence detection unit 4 starts to work, the emitted laser excites aerosol particles in the sampling liquid, the first fluorescence detector 413 and the second fluorescence detector 409 respectively obtain a first channel intrinsic fluorescence signal and a second channel intrinsic fluorescence signal, after continuous irradiation for a plurality of seconds, the intrinsic fluorescence intensity may be attenuated to generate a bleaching phenomenon, the attenuation speeds of different biological aerosol particles are different, and the first fluorescence detector 413 and the second fluorescence detector 409 obtain a bleached first fluorescence signal and a bleached second fluorescence signal;
4) After fluorescence detection is finished, the liquid outlet peristaltic pump 6 starts to work, under the drive of the liquid outlet peristaltic pump 6, sampling liquid passes through the particle filtering unit 5, aerosol particles in the sampling liquid can be intercepted by the particle filtering unit 5, purer sampling liquid returns to the liquid storage unit 7, and the sampling liquid is reserved for a subsequent period to be used as new sampling liquid;
5) The particle concentration value and the fluorescence information are sent to the central control unit 9, so that the central control unit 9 judges the biological attribute and the category of the aerosol particles according to the collected particle concentration value and fluorescence information.
The device can periodically perform sampling and monitoring work for a long time, and when the sampling liquid in the liquid storage unit 7 is insufficient, fresh sampling liquid needs to be supplemented.
Experiments show that the device concentrates the bioaerosol particle sample by a high-flow liquid phase sampling technology, monitors the intrinsic fluorescence of the bioaerosol particles by an ultraviolet induced intrinsic fluorescence technology, and can realize high-sensitivity and rapid detection of the bioaerosol particles with low concentration; the device comprises a closed loop liquid path system of the particle filter, so that the sampling liquid can be reused for a plurality of times, and the long-time automatic monitoring of the monitoring device is realized. The device can detect and obtain multiple information such as particle concentration, intrinsic fluorescence and bleached intrinsic fluorescence of the enriched biological aerosol particle sample, and accordingly, the detection of the low false alarm rate and the primary classification of aerosol particles can be realized.
Claims (4)
1. The high-flow biological aerosol intrinsic fluorescence monitoring device is characterized by comprising a high-flow sampling fan (1), a sampling cup (2) filled with liquid phase medium, a particle counter (3) arranged at an air inlet end, a fluorescence detection unit (4), a particle filtering unit (5), a liquid outlet peristaltic pump (6), a liquid storage unit (7), a liquid inlet peristaltic pump (8) and a central control unit (9);
the sampling cup (2) is integrally formed by an upper section cylinder and a lower section cone cup shell-shaped structure, the cone cup shell-shaped structure is used for containing sampling liquid, the cylindrical upper end of the sampling cup (2) is communicated with the large-flow sampling fan (1) to form a cyclone sampling device, an airflow connecting pipe (21) is arranged at a lower position of one side surface of the cylindrical upper section of the sampling cup (2), the particle counter (3) is connected to one side of the airflow connecting pipe (21), a liquid inlet (22) is arranged at a higher position of the other side surface of the cylindrical upper section of the sampling cup (2), an ultraviolet-visible light antireflection film is plated on the surface of the cone cup shell of the sampling cup (2), the cone tip end of the cone cup shell of the sampling cup (2) is communicated with the liquid storage unit (7) through a particle filtering unit (5) and a liquid peristaltic pump (6), and the other liquid storage pipe at the outlet of the liquid storage unit (7) is communicated with the liquid inlet (22) of the sampling cup (2) through the liquid inlet (8);
the fluorescence detection unit (4) comprises an ultraviolet light emitting diode (401), a shaping optical component (402), a detection area of a cone-shaped shell of the sampling cup (2) and a light trap (403) are sequentially arranged along the laser output direction of the ultraviolet light emitting diode (401), aerosol particles in the detection area emit intrinsic fluorescence under the irradiation of laser of the ultraviolet light emitting diode (401), the intrinsic fluorescence is collected by a focusing lens group (404) and then is parallelly incident to a dichroic mirror (405), short-wave fluorescence of the intrinsic fluorescence is reflected by the dichroic mirror (405) and then sequentially passes through a short-wave filter (410), a short-wave focusing lens (411) and a first diaphragm (412) and then reaches a first fluorescence detector (413), and long-wave fluorescence of the intrinsic fluorescence sequentially passes through the dichroic mirror (405) and then sequentially passes through a long-wave filter (406), a long-wave focusing lens (407) and a second diaphragm (408) and then reaches a second fluorescence detector (409).
2. The high-flow bioaerosol intrinsic fluorescence monitoring apparatus as claimed in claim 1, wherein the detection zone (i.e. the light sensitive zone) of the fluorescence detection unit (4) is located in the lower half of the sample liquid of the sampling cup (2).
3. The high-flow biological aerosol intrinsic fluorescence monitoring device according to claim 1, wherein the central control unit (9) is used for controlling the start and stop of the sampling fan (1), the particle counter (3), the fluorescence detection unit (4), the liquid outlet peristaltic pump (6) and the liquid inlet peristaltic pump (8), and receiving detection results from the particle counter (3) and the fluorescence detection unit (4).
4. The high-flow bioaerosol intrinsic fluorescence monitoring apparatus as claimed in claim 1, wherein the particle filter unit (5) is a high-efficiency filter suitable for liquid, has a filter particle diameter of not less than 0.22 μm, can filter most of microorganism particles, and has a filter efficiency of not less than 99.9%.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202210105446.5A CN116559125A (en) | 2022-01-28 | 2022-01-28 | Large-flow biological aerosol intrinsic fluorescence monitoring device |
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| CN202210105446.5A CN116559125A (en) | 2022-01-28 | 2022-01-28 | Large-flow biological aerosol intrinsic fluorescence monitoring device |
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| CN116559125A true CN116559125A (en) | 2023-08-08 |
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| CN202210105446.5A Pending CN116559125A (en) | 2022-01-28 | 2022-01-28 | Large-flow biological aerosol intrinsic fluorescence monitoring device |
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- 2022-01-28 CN CN202210105446.5A patent/CN116559125A/en active Pending
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