CN207051160U - A kind of sorting type flow cytometer - Google Patents
A kind of sorting type flow cytometer Download PDFInfo
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- CN207051160U CN207051160U CN201720532560.0U CN201720532560U CN207051160U CN 207051160 U CN207051160 U CN 207051160U CN 201720532560 U CN201720532560 U CN 201720532560U CN 207051160 U CN207051160 U CN 207051160U
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Abstract
It the utility model is related to a kind of sorting type flow cytometer, it is characterised in that including liquid fluid system, light path system, testing and analysis system and separation system；Liquid fluid system is used to make it that sheath fluid parcel sample flow is sprayed；Light path system includes Optical Maser System, condenser, pin hole, collimation lens, optical filter, optical-electrical converter and shield bars, sample in the laser irradiation sample flow that Optical Maser System is sent produces scattering light, it is provided for converging the condenser of sample forward scattering light with the rear of laser light incident direction identical sample flow, forward scattering light after focusing is transmitted into collimation lens through needle passing hole, the directional light of collimated lens outgoing by optical filter filter out it is spuious penetrate light after be transmitted into optical-electrical converter, optical-electrical converter is used to forward scattering light being changed into electric signal and is sent to testing and analysis system after amplifying, the front of condenser sets lighttight shield bars with the coplanar position of laser beam incident；Separation system is used to sort the sample with electric charge.
Description
Technical field
The utility model is on a kind of sorting type flow cytometer, is related to technical field of biological.
Background technology
Flow cell sorter is that multi-parameter qualitative and quantitative analysis is carried out to the single-row particle in streamlined flow state and is divided
The method of choosing, this method have high speed, high flux, high activity, precisely from the horizontal detection of individual particle and the characteristics of sort sample.Stream
The detection parameters of formula cell sorter include scattering light and fluorescence signal.Scattered light signal includes preceding to (FSC) and lateral scattering
Optical signal (SSC).Forward-scattering signal is mainly for detection of sample size, and side scattered light is mainly for detection of sample knot
Structure.
The forward scattering luminous sensitivity and resolution ratio of ordinary stream cell sorter are low, well below the spirit of side scattered light
Sensitivity and resolution ratio.Sensitivity refers to the detectable smallest particles sample of forward scattering light, and it is distinguishable that resolution ratio refers to forward scattering light
Minimum difference sample.The sensitivity of ordinary stream cell sorter forward scattering light is 0.5 μm, and resolution ratio is 0.1 μm, and
Sample often reduces 1 times, and caused scattered light intensity can reduce 6 times, so it is small to diameter to greatly limit flow cell sorter
In 0.5 μm, difference is less than the detection and sorting of 0.1 μm of little particle sample, and this includes such as artificial nano particle, microorganism, extracellular
Vesica, organelle equal samples.Therefore, design can expand existing with high sensitivity, high-resolution forward scattering photodetector
The application field of flow cell sorter, realize the mesh of flow cell sorter detection and sorting fine difference little particle sample
's.In addition, when detecting little particle sample of the diameter less than less than 0.5 μm, the liquid fluid system of ordinary stream cell sorter is deposited
In problems with：Sheath fluid filtration system can only remove the impurity that particle is larger in sheath fluid.Because miscellaneous particle and little particle sample
Sizableness, so the forward-scattering signal of miscellaneous particle can hide the forward-scattering signal of little particle sample, increase
The ratio of invalid granule number when having added laser detection, so as to reduce the efficiency and accuracy of detection and sorting.
Utility model content
In view of the above-mentioned problems, the purpose of this utility model is to provide a kind of high-resolution and highly sensitive sorting type streaming
Cell instrument.
To achieve the above object, the utility model takes following technical scheme：A kind of sorting type flow cytometer, its feature
It is, the flow cytometer includes liquid fluid system, light path system, testing and analysis system and separation system；The liquid fluid system is used
Wrap up sample flow in causing sheath fluid and sprayed；The light path system include Optical Maser System, condenser, pin hole, collimation lens,
Optical filter, optical-electrical converter and shield bars, the sample that the laser that the Optical Maser System is sent is irradiated in the sample flow produce
Light is scattered, is provided for converging the described poly- of sample forward scattering light with the rear of sample flow described in the identical of laser light incident direction
Light microscopic, the pin hole is set in the rear focus of the condenser, the forward scattering light after focusing is transmitted into by the pin hole
The collimation lens, through the directional light of collimation lens outgoing filtered out by the optical filter it is spuious penetrate light after be transmitted into it is described
Optical-electrical converter, the optical-electrical converter are used to forward scattering light being changed into electric signal and are sent to the detection point after amplifying
Analysis system, front and the coplanar position of laser beam incident of the condenser set the lighttight shield bars；It is described
Separation system is used to sort the sample with electric charge.
Further, the liquid fluid system includes positive pressure pump, sheath fluid bucket, bag type filter component, sample pipeline, sample
Pipe, nozzle, liquid waste collector, waste liquid barrel and negative pressure pump；The positive pressure pump connects the air inlet of the sheath fluid bucket by gas circuit pipe,
The sheath fluid bucket connects the bag type filter component by sheath liquid pipeline, sheath fluid after the bag type filter component filters simultaneously
Enter the nozzle through sheath liquid pipeline and form sheath liquid stream；The sample tube connects the nozzle, the sample by sample pipeline
Sample in pipe enters the nozzle through sample pipeline and forms sample flow；The sheath liquid stream wraps up sample flow together from the nozzle
Place sprays, and the last sheath liquid stream is wrapped in sample and flows through the liquid waste collector inflow waste liquid barrel, and the waste liquid barrel is also
The negative pressure pump is connected by waste collection gas circuit pipe.
Further, the bag type filter component is used 0.22 μm of bag type filter and 0.1um bag type filter strings
Connection, the sheath fluid is set to be filtered successively by 0.22 μm of bag type filter filtering and 0.1 μm of bag type filter.
Further, the separation system includes ultrasonic liquid stream oscillator, voltage deflection plate and separation and collection pipe；It is described super
Sound liquid stream oscillator is fixedly installed on above the nozzle, for the parcel sample flow vibration of sheath liquid stream to be fragmented into single drop,
The voltage deflection plate is used to enter horizontal deflection to the sample with electric charge, and the sample after sorting enters the sorting and received
Collector.
Further, the condenser uses the object lens of high power numerical aperture as 20 × (0.45NA), is less than for collecting
Forward scattering light in the range of 30 °.
Further, the shield bars use opaque black aluminum material, and the shield bars are shaped as rectangle, institute
State that the length of shield bars is identical with the diameter of the condenser, the width of the shield bars is 3~7mm.
Further, the laser of the Optical Maser System uses wavelength as 405nm, laser power 100mW, the filter
The centre wavelength of mating plate matches with the laser wavelength, and the optical filter uses bandpass filter.
Further, the optical-electrical converter uses photomultiplier or avalanche photodide with enlarging function.
Further, the sorting type flow cytometer also includes a polarized light detection for being arranged on the collimation lens rear
Element.
Further, the nozzle uses 100 μm of nozzles, and sheath hydraulic coupling is 7psi, the liquid of the ultrasonic liquid stream oscillator
Stream frequency of oscillation is 19~20kHz, and loading speed is 5000/second.
For the utility model due to taking above technical scheme, it has advantages below：1st, in order to improve forward scattering light
Detection sensitivity is, it is necessary to miscellaneous particle of the removal from sheath fluid as much as possible, and the utility model is using 0.22 μm and 0.1 μm string
The bellows filter of connection, sheath liquid stream is set successively by 0.22 μm and 0.1 μm of bellows filter filtering, to filter most of from the straight of sheath fluid
Footpath is more than 0.1 μm of noise particle, while extends the service life of filter, before can so reducing in sheath fluid caused by noise particle
To scattered light signal, the ratio of invalid granule number when reducing laser detection, so as to improve the efficiency and accuracy of detection and sorting,
The detection sensitivity of forward scattering light is improved, 80nm, resolution ratio can be reached by being experimentally confirmed sensitivity of the present utility model
20nm can be reached.2nd, the utility model is less than 0.5 μm of little particle sample only by forward scattering light can detection diameter,
And the sample of 20nm differences is made a distinction and sorted, therefore expand the application field of flow cell sorter.3rd, this practicality
The new angle of blocking by increasing shield bars, it can further weaken the noise signal of laser and diffraction ring, be carried so as to reach
The purpose of high s/n ratio.4th, when detecting little particle sample of the diameter less than 0.5 μm, the utility model is by forward scattering light through poly-
After light microscopic convergence, whole forward scattering light through pin hole are collected into optical-electrical converter, improve the collection of forward scattering light, so as to
Improve the detection sensitivity and resolution ratio of forward scattering light.The utility model is examined especially for the difference of unstressed configuration marker samples
Survey and sort and be significant.
Brief description of the drawings
Fig. 1 is the overall structure diagram of sorting type flow cytometer of the present utility model；
Fig. 2 is the pipeline connection diagram of sheath liquid stream and sample flow of the present utility model；
Fig. 3 is light path system structural representation of the present utility model；
Fig. 4 is the design sketch of forward scattering light detection polystyrene material microballoon of the present utility model；
Fig. 5 is the design sketch of the present utility model sorted using forward scattering light before and after polystyrene material microballoon, wherein,
Fig. 5 A are result before sorting, and Fig. 5 B and C are result after sorting.
Embodiment
Come to carry out the utility model detailed description below in conjunction with accompanying drawing.It should be appreciated, however, that accompanying drawing is provided only
The utility model is more fully understood, they should not be understood paired limitation of the present utility model.
As shown in Figures 1 to 3, sorting type flow cytometer provided by the utility model includes liquid fluid system, light path system, inspection
Survey analysis system and separation system.Wherein, liquid fluid system includes positive pressure pump 1, gas circuit pipe 2, sheath fluid bucket 3, bag type filter component
4th, sample pipeline 5, sample tube 6, sheath liquid pipeline 7, nozzle 8, liquid waste collector 9, waste collection pipeline 10, waste liquid barrel 11, waste liquid
Collect gas circuit pipe 12 and negative pressure pump 13；Light path system includes Optical Maser System 14, condenser 15, pin hole 16, collimation lens 17, filter
Mating plate 18, optical-electrical converter 19 and shield bars 20；Testing and analysis system can use computer 21；Separation system includes ultrasonic liquid
Flow oscillator 22, voltage deflection plate 23 and separation and collection pipe 24.
Positive pressure pump 1 connects the air inlet of sheath fluid bucket 3 by gas circuit pipe 2, and sheath fluid bucket 3 is used to hold sheath fluid, and sheath fluid bucket 3 is logical
Cross sheath liquid pipeline 7 and connect bag type filter component 4, sheath fluid enters spray after the filtering of bag type filter component 4 and through sheath liquid pipeline 7
Mouth 8 forms sheath liquid stream；Sample tube 6 is used to hold sample, and sample tube 6 connects nozzle 8 by sample pipeline 5, the sample in sample tube 6
This enters nozzle 8 through sample pipeline 5 and forms sample flow；Sheath liquid stream parcel sample flow together sprays at nozzle 8, last sheath liquid stream
It is wrapped in sample flow and flows into waste liquid barrel 11 through waste collection pipeline 10 into liquid waste collector 9, waste liquid barrel 11 is also received by waste liquid
Collect gas circuit pipe 12 and connect negative pressure pump 13.
Sample in the laser irradiation sample flow that Optical Maser System 14 is sent produces scattering light, identical with laser light incident direction
Scattering light be forward scattering light, condenser 15 is set with the rear of laser light incident direction identical sample flow, condenser 15 is used
In the forward scattering light of convergence sample, the position of sample flow is adjusted, tested sample is located in the object focus of condenser 15, gathered
Pin hole 16 is set in the rear focus of light microscopic 15, pin hole 16 is used to remove miscellaneous signal beyond object space focal point, after focusing before
Collimation lens 17 is transmitted into after needle passing hole 16 to scattering light, the incident light of diverging is collimated into directional light by collimation lens 17, warp
The directional light that collimation lens 17 is emitted filtered out by optical filter 18 beyond the excitation wavelength of laser it is spuious penetrate light after be transmitted into light
Electric transducer 19, optical-electrical converter 19 are used to forward scattering light is changed into electric signal and amplified to be sent to computer 21, in addition,
Lighttight shield bars 20, black shield bars 20 are installed in the front of condenser 15 and with the coplanar position of laser beam incident
For preventing laser and its diffraction ring from inciding in condenser 15, prevent from causing the phenomenon for covering forward-scattering signal.
Ultrasonic liquid stream oscillator 22 is fixedly installed on the top of nozzle 8, for the parcel sample flow vibration of sheath liquid stream to be fragmented into
Single drop, voltage deflection plate 23 are used to enter horizontal deflection to the sample with electric charge, and the sample after sorting enters separation and collection pipe
24。
In a preferred embodiment, as shown in Fig. 2 in order to further improve the sensitivity of scattering light detection, remove
Noise signal from sheath fluid, while extend the service life of filter, bag type filter component 4 of the present utility model uses
By 0.22 μm of bag type filter 41 and 0.1 μm of bag type filter series connection 42, sheath fluid is set to pass through 0.22 μm of bag type filter mistake successively
41 and 0.1 μm of bag type filter filterings 42 of filter, filter the noise particle that most of diameter for coming from sheath fluid is more than 0.1 μm.This
Utility model causes sheath fluid noise signal to be reduced to average 400/second by using two bag type filters, although also having certain
The noise signal of ratio, but it is relatively low relative to sample speed more than average 5000/second, the noise level of forward scattering light.
As can be seen here, the utility model can carry the polystyrene microsphere of the lower limit of forward scattering photodetector from 0.2 μm of diameter of detection
Height is to 0.1 μm or so.
In a preferred embodiment, 0.5 μm of little particle sample is less than for diameter, the forward scattering light of sample becomes
It is distributed in wide-angle, and it is smaller to detect sample, forward scattering light distribution is closer to spherical, in addition, little particle sample itself
Caused forward scattering luminous intensity is weaker.So if using low-angle capture range condenser, can cause collect sample
Forward scattering luminous intensity is weaker, the forward-scattering signal of little particle sample is submerged in background noise.In order to improve
The detection sensitivity and resolution ratio of forward scattering light are, it is necessary to increase the collection angle of condenser 15, condenser of the present utility model
15 use the object lens of high power numerical aperture as 20 × (0.45NA) (0.45 refers to the numerical value of numerical aperture), collect and are less than 30 °
In the range of forward scattering light, enhance the intensity of little particle sample forward-scattering signal, improve before little particle sample to
Scatter the detection sensitivity and resolution ratio of light.
In a preferred embodiment, because laser and its diffraction ring noise signal are very strong, so even if very little ratio
The laser and its diffraction ring noise signal of example, which are drained in condenser 15, will also result in signal to noise ratio reduction, influence forward scattering light
Detection resolution.The application is used to increase when detecting little particle sample of the diameter less than 0.5 μm using the shield bars 20 of broadening type
Big shield bars block angle, and shield bars 20 use opaque black aluminum material, are shaped as rectangle, length and condenser
Diameter is all mutually 2.5cm, and width can be 3~7mm, the signal to noise ratio of forward scattering light detection can be effectively improved, wherein optimal
Width be 5mm, shield bars 20 block angle and can reach 15 °, i.e. the optical signal less than 15 ° is not received by condenser 15,
The forward scattering light of condenser 15 receptions, 15 °~30 ° scopes, so as to improve the signal to noise ratio of forward scattering light detection, further
Improve the resolution ratio of forward scattering light.
In a preferred embodiment, the detection that forward scattering light can be improved due to the exciting light of shorter wavelength is differentiated
Rate, therefore the laser wavelength of Optical Maser System of the present utility model 14 selects 405nm, laser power 100mW, optical filter 18
405 ± 5nm of bandpass filter is selected, makes the forward scattering light that 405nm ± 5nm wavelength can only be received in optical-electrical converter 19, goes
Except the miscellaneous particle signal of other wavelength.
In a preferred embodiment, optical-electrical converter 19 can use photomultiplier or snow with enlarging function
Avalanche photo diode etc., so as to improve the detection sensitivity of forward scattering light and resolution ratio, using photoelectricity in the present embodiment
Multiplier tube.
In a limited embodiment, when sorting type flow cytometer of the present utility model needs to have Polarization Detection work(
During energy, the polarizability of sample forward scattering light is detected, the member of polarized light detection can be provided at the rear of collimation lens 17
Part, original forward scattering light is divided into the forward scattering light of vertically and horizontally two direction of vibration, then respectively by two photoelectricity
Converter receives.
The stabilization of liquid stream breakpoint, low sheath hydraulic coupling need when in a preferred embodiment, in order to ensure sample sorting
Use larger nozzle.The nozzle 8 of the utility model embodiment uses 100 μm of nozzles, and sheath hydraulic coupling is 7psi, and ultrasonic liquid stream is shaken
The liquid stream frequency of oscillation for swinging device 22 is 19~20kHz, and loading speed is 5000/second, i.e., single sample receives laser irradiation
Time is about 0.2 millisecond.
In a preferred embodiment, miscellaneous be eliminated as much as in sheath liquid pipeline 7 and sample pipeline 5 is needed before experiment
Grain, sheath fluid pipeline 7 and sample pipeline 5 about 20 minutes are rinsed at a high speed using 75% ethanol first, then with the ultra-pure water after sterilizing
Sheath liquid pipeline and sample pipeline about 30 minutes are rinsed, is eventually adding the sheath fluid newly configured after 0.1 μm of aperture membrane filtration.
In a preferred embodiment, when flow cell sorter sorts, in order to be added dropwise to the sheath fluid of parcel sample
Electricity, so that the powered sheath fluid drop of parcel sample deflects in the electric field, separation and collection pipe 24 is later fallen into, so this practicality
New sheath fluid uses the most frequently used phosphate buffer, and in order to detect the little particle sample for being less than 0.5 μm, sheath fluid will before experiment
Through 0.1 μm of membrane filtration, and matching while using, if placed for a long time, larger particulate matter, increase noise letter can be formed in sheath fluid
Number.
In a preferred embodiment, when detection is less than 0.5 μm of little particle sample, for each little particle sample
Fully detected, it is necessary to increase the time of each little particle sample of laser detection, so selecting relatively low sheath hydraulic coupling and sample
Pressure, the sheath hydraulic coupling 7psi that the utility model is set or so, sample are usually no more than 0.5psi higher than the pressure differential of sheath fluid.
In summary, sorting type flow cytometer of the present utility model substantially increase before ordinary stream cell sorter to
Scatter the detection sensitivity and resolution ratio of light.Forward scattering light detection minimum detectable diameter 80nm polystyrene microsphere, and
Diameter is differed to the 20nm polystyrene material microballoon of diameter 130,150,170 using forward scattering light realizes baseline separation
(Fig. 4).In addition, also sorting out the diameter 130 of mixing and 150nm polystyrene microspheres, separating purity reaches more than 90%,
Wherein, Fig. 5 A are result before sorting, and Fig. 5 B and C are result after sorting.
The various embodiments described above are merely to illustrate the utility model, wherein each optical element can use conventional support to carry out
Support is fixed, and position of optical element etc. can be all varied from, as long as meeting paths bar of the present utility model
Part, in addition, the structure of each part, connected mode and manufacture craft etc. can be all varied from, it is every in this practicality
The equivalents carried out on the basis of new technique scheme and improvement, should not exclude the scope of protection of the utility model it
Outside.
Claims (10)
1. a kind of sorting type flow cytometer, it is characterised in that the flow cytometer includes liquid fluid system, light path system, detection
Analysis system and separation system；
The liquid fluid system is used to make it that sheath fluid parcel sample flow is sprayed；The light path system includes Optical Maser System, gathered
Light microscopic, pin hole, collimation lens, optical filter, optical-electrical converter and shield bars, described in the irradiation of laser that the Optical Maser System is sent
Sample in sample flow produces scattering light, is provided for converging sample with the rear of sample flow described in the identical of laser light incident direction
The condenser of forward scattering light, the pin hole is set in the rear focus of the condenser, the forward scattering light after focusing
The collimation lens is transmitted into by the pin hole, the directional light through collimation lens outgoing filters out miscellaneous by the optical filter
The optical-electrical converter is transmitted into after scattering light, the optical-electrical converter is used to forward scattering light is changed into electric signal and amplified
After be sent to the testing and analysis system, the front of the condenser and the coplanar position of laser beam incident set light tight
The shield bars；The separation system is used to sort the sample with electric charge.
2. a kind of sorting type flow cytometer as claimed in claim 1, it is characterised in that the liquid fluid system includes malleation
Pump, sheath fluid bucket, bag type filter component, sample pipeline, sample tube, nozzle, liquid waste collector, waste liquid barrel and negative pressure pump；It is described
Positive pressure pump connects the air inlet of the sheath fluid bucket by gas circuit pipe, and the sheath fluid bucket connects the bellows by sheath liquid pipeline and filtered
Device assembly, sheath fluid enter nozzle formation sheath liquid stream after the bag type filter component filters and through sheath liquid pipeline；It is described
Sample tube connects the nozzle by sample pipeline, and the sample in the sample tube enters the nozzle through sample pipeline and forms sample
This stream；The sheath liquid stream parcel sample flow together sprays at the nozzle, and the last sheath liquid stream is wrapped in sample and flows through institute
State liquid waste collector and flow into the waste liquid barrel, the waste liquid barrel also connects the negative pressure pump by waste collection gas circuit pipe.
3. a kind of sorting type flow cytometer as claimed in claim 2, it is characterised in that the bag type filter component uses
By 0.22 μm of bag type filter and 0.1 μm of bag type filter series connection, the sheath fluid is set to pass through 0.22 μm of bag type filter mistake successively
Filter and 0.1 μm of bag type filter filter.
4. a kind of sorting type flow cytometer as claimed in claim 2 or claim 3, it is characterised in that the separation system includes super
Sound liquid stream oscillator, voltage deflection plate and separation and collection pipe；The ultrasonic liquid stream oscillator is fixedly installed on above the nozzle,
For the parcel sample flow vibration of sheath liquid stream to be fragmented into single drop, the voltage deflection plate is used for the sample with electric charge
Originally horizontal deflection is entered, the sample after sorting enters the separation and collection pipe.
5. a kind of sorting type flow cytometer as described in claim 1 or 2 or 3, it is characterised in that the condenser is using high
The object lens 20 in multiple value aperture × (0.45NA), for collecting the forward scattering light being less than in the range of 30 °.
6. a kind of sorting type flow cytometer as described in claim 1 or 2 or 3, it is characterised in that the shield bars are not using
Transparent black aluminum material, the shield bars are shaped as rectangle, the length of the shield bars and the diameter of the condenser
Identical, the width of the shield bars is 3~7mm.
7. a kind of sorting type flow cytometer as described in claim 1 or 2 or 3, it is characterised in that the Optical Maser System
Laser uses wavelength as 405nm, laser power 100mW, the centre wavelength of the optical filter and the laser wavelength phase
Matching, the optical filter use bandpass filter.
8. a kind of sorting type flow cytometer as described in claim 1 or 2 or 3, it is characterised in that the optical-electrical converter is adopted
With photomultiplier or avalanche photodide with enlarging function.
A kind of 9. sorting type flow cytometer as described in claim 1 or 2 or 3, it is characterised in that the sorting type fluidic cell
Instrument also includes a polarized light detection element for being arranged on the collimation lens rear.
10. a kind of sorting type flow cytometer as claimed in claim 4, it is characterised in that the nozzle is using 100 μm of sprays
Mouth, sheath hydraulic coupling are 7psi, and the liquid stream frequency of oscillation of the ultrasonic liquid stream oscillator is 19~20kHz, and loading speed is 5000
Individual/second.
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| CN201720532560.0U CN207051160U (en) | 2017-05-15 | 2017-05-15 | A kind of sorting type flow cytometer |
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| CN201720532560.0U CN207051160U (en) | 2017-05-15 | 2017-05-15 | A kind of sorting type flow cytometer |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107167416A (en) * | 2017-05-15 | 2017-09-15 | 中国科学院微生物研究所 | A kind of sorting type flow cytometer |
| CN108593530A (en) * | 2018-06-01 | 2018-09-28 | 大连海事大学 | Microparticle forward scattering optical detection device and its detection method |
| WO2019209713A1 (en) | 2018-04-27 | 2019-10-31 | Becton, Dickinson And Company | Collection systems for flow cytometrically sorted samples and methods of using the same |
| CN110411933A (en) * | 2019-08-22 | 2019-11-05 | 合肥京东方光电科技有限公司 | Forward scattering optical detection system, flow cytometer and the method for measuring cell dia |
| CN112136033A (en) * | 2018-04-27 | 2020-12-25 | 贝克顿·迪金森公司 | Flow cytometer with enclosed droplet sorter with controlled aerosol content and method of using same |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107167416A (en) * | 2017-05-15 | 2017-09-15 | 中国科学院微生物研究所 | A kind of sorting type flow cytometer |
| CN107167416B (en) * | 2017-05-15 | 2023-07-07 | 中国科学院微生物研究所 | Sorting type flow cytometer |
| WO2019209713A1 (en) | 2018-04-27 | 2019-10-31 | Becton, Dickinson And Company | Collection systems for flow cytometrically sorted samples and methods of using the same |
| CN112136033A (en) * | 2018-04-27 | 2020-12-25 | 贝克顿·迪金森公司 | Flow cytometer with enclosed droplet sorter with controlled aerosol content and method of using same |
| JP2021521840A (en) * | 2018-04-27 | 2021-08-30 | ベクトン・ディキンソン・アンド・カンパニーＢｅｃｔｏｎ， Ｄｉｃｋｉｎｓｏｎ Ａｎｄ Ｃｏｍｐａｎｙ | Collection system for samples sorted by flow cytometry and how to use it |
| EP3785017A4 (en) * | 2018-04-27 | 2022-01-26 | Becton, Dickinson and Company | Collection systems for flow cytometrically sorted samples and methods of using the same |
| US11275075B2 (en) | 2018-04-27 | 2022-03-15 | Becton, Dickinson And Company | Collection systems for flow cytometrically sorted samples and methods of using the same |
| JP7374124B2 (en) | 2018-04-27 | 2023-11-06 | ベクトン・ディキンソン・アンド・カンパニー | Collection system for flow cytometrically sorted samples and methods of using it |
| CN108593530A (en) * | 2018-06-01 | 2018-09-28 | 大连海事大学 | Microparticle forward scattering optical detection device and its detection method |
| CN110411933A (en) * | 2019-08-22 | 2019-11-05 | 合肥京东方光电科技有限公司 | Forward scattering optical detection system, flow cytometer and the method for measuring cell dia |
| US11898951B2 (en) | 2019-08-22 | 2024-02-13 | Hefei Boe Optoelectronics Technology Co., Ltd. | Forward scattered light detection system, flow cytometer and method for measuring cell diameter |
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