CN116549362A - Jasmine flower composition fermentation filtrate and preparation method and application thereof - Google Patents
Jasmine flower composition fermentation filtrate and preparation method and application thereof Download PDFInfo
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- CN116549362A CN116549362A CN202310835805.7A CN202310835805A CN116549362A CN 116549362 A CN116549362 A CN 116549362A CN 202310835805 A CN202310835805 A CN 202310835805A CN 116549362 A CN116549362 A CN 116549362A
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- jasmine
- drying
- calendula
- fermentation
- jasmine flower
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/005—Preparations for sensitive skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/84—Products or compounds obtained by lyophilisation, freeze-drying
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Dermatology (AREA)
- Microbiology (AREA)
- Epidemiology (AREA)
- Birds (AREA)
- Mycology (AREA)
- Botany (AREA)
- Biotechnology (AREA)
- Engineering & Computer Science (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention provides a jasmine flower composition fermentation filtrate, which comprises the following raw materials: jasmine, calendula, camellia, chamomile and bacterial species; the strain is lactobacillus pentosus and lactococcus garvieae; the drying mode of the jasmine flower is as follows: rapidly freezing flos Jasmini sambac at-35deg.C for 1min, and freeze drying at-45deg.C under 18Pa for 1min; and then placing the freeze-dried jasmine flower into microwaves for drying, wherein the microwave power is 500W, and the microwave time is 5s. The fermentation filtrate provided by the invention has excellent effects of relieving allergy, moisturizing, brightening, resisting wrinkles, resisting oxidization and maintaining beauty and keeping young, and the absorption of the fermentation filtrate is very strong.
Description
Technical Field
The invention relates to the technical field of fermentation, in particular to jasmine flower composition fermentation filtrate and a preparation method and application thereof.
Background
Jasmine (Jasminum sambac (L.) Ait.) belongs to genus Jasminum of Oleaceae, and there are 706 kinds of jasmine all over the world, which are well known scented tea raw materials and important essence raw materials. The flower, leaf and root of jasmine flower have medicinal value and have the activities of resisting oxidation, depression, bacteria, fungi, viruses and the like. The jasmine essential oil is praised as the king of essential oil, has elegant smell, can relax emotion, arouse spirit, improve the dry skin, lack water, oil and sensitive conditions, and lighten scars and striae gravidarum.
Calendula is a main raw material for extracting pure natural yellow pigment, and the natural yellow pigment is widely applied to the fields of food industry, feed, medicine and the like and chemical industry, and is an important additive. Studies have shown that methanol extracts of the tongue and tubular flowers of calendula have an inhibitory effect on TPA-induced auricle inflammation in mice. The flos Camelliae Japonicae is flower of Camellia japonica of Theaceae. Can be used for cooling blood, stopping bleeding, removing blood stasis, and relieving swelling. It is indicated for hematemesis, epistaxis, metrorrhagia, intestinal wind, bloody dysentery, stranguria with blood, traumatic injury, scald, etc.
The product produced after the strain fermentation contains rich amino acids, polypeptides, various vitamins and minerals, and the substances have indispensable functions in skin care, and can have good whitening, moisturizing and other effects through different ways.
Aiming at the situation, the invention provides a jasmine flower composition fermentation filtrate and a preparation method and application thereof.
Disclosure of Invention
The invention provides a jasmine flower composition fermentation filtrate, which comprises the following raw materials: jasmine, calendula, camellia, chamomile and bacterial species;
the strain is lactobacillus pentosus and lactococcus garvieae;
the drying mode of the jasmine flower is as follows: rapidly freezing flos Jasmini sambac at-35deg.C for 1min, and freeze drying at-45deg.C under 18Pa for 1min; and then placing the freeze-dried jasmine flower into microwaves for drying, wherein the microwave power is 500W, and the microwave time is 5s.
In another aspect, the invention provides a method for preparing the jasmine flower composition fermentation filtrate, which comprises the following steps:
s01, respectively selecting jasmine, calendula, camellia and chamomile, cleaning, drying, crushing and sieving to obtain powder;
s02, mixing the powder obtained in the step S01 with a liquid culture medium;
s03, inoculating a strain into the mixed liquid culture medium obtained in the step S02 for fermentation, and then filtering to obtain a jasmine flower composition fermentation filtrate.
Preferably, the calendula is dried by freeze drying and then microwave drying.
Preferably, the drying mode of the camellia and the golden chamomile is microwave drying.
Preferably, in S02, the components of the liquid medium are: 11.0g of casein peptone, 8.0g of beef extract, 5.0g of yeast powder, 5.0g of glucose, 5.0g of sodium acetate, 3.0g of acetic acid, 2.0g of diammonium citrate and 0.5g,Tween 80 1.0g,K g of citric acid 2 HPO 4 2.0g，MgSO 4 ·7H 2 O 0.2g，MnSO 4 ·H 2 O 0.05g，CaCO 3 20.0g, 15.0g of agar and 1.0L of distilled water.
Preferably, the inoculum size of the strain is 6-12wt%.
Preferably, the fermentation conditions are: introducing oxygen at 35+ -2deg.C, and fermenting for 48 hr.
Preferably, the composition comprises any one or more of glycerol, erythritol, trehalose, glycerol glucoside, beta-glucan, tremella polysaccharide, 1, 2-hexanediol, 1, 2-pentanediol.
In another aspect, the invention provides a skin care product comprising the jasmine composition fermentation filtrate.
By adopting the technical scheme, the invention has the following beneficial effects:
the fermentation filtrate provided by the invention has excellent effects of relieving allergy, moisturizing, brightening, resisting wrinkles, resisting oxidization and maintaining beauty and keeping young, and the absorption of the fermentation filtrate is very strong.
Drawings
In order to more clearly illustrate the embodiments of the invention or the technical solutions in the prior art, the drawings which are required in the description of the embodiments or the prior art will be briefly described, it being obvious that the drawings in the description below are some embodiments of the invention and that other drawings may be obtained from these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a test chart of group 1 in Performance test 5;
fig. 2 is a test chart of group 4 in performance test 5.
Detailed Description
The following description of the present invention will be made clearly and fully, and it is apparent that the embodiments described are some, but not all, of the embodiments of the present invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
The invention provides a jasmine flower composition fermentation filtrate, which comprises the following raw materials: jasmine, calendula, camellia, chamomile and bacterial species; the strain is selected from one or more of Lactobacillus, lactococcus and Saccharomyces.
Preferably, the bacterial species are lactobacillus pentosus and lactococcus garvieae.
Lactobacillus pentosus Lactiplantibacillus pentosus is purchased from China industry microbiological culture Collection center, and the strain deposit number CICC 20301; lactococcus garvieae Lactococcus garvieae is purchased from China industry microbiological culture Collection center, with strain deposit number CICC cube 21038.
In another aspect, the invention provides a method for preparing the jasmine flower composition fermentation filtrate, which comprises the following steps:
s01, respectively selecting jasmine, calendula, camellia and chamomile, cleaning, drying, crushing and sieving to obtain powder;
s02, mixing the powder obtained in the step S01 with a liquid culture medium;
s03, inoculating a strain into the mixed liquid culture medium obtained in the step S02 for fermentation, and then filtering to obtain a jasmine flower composition fermentation filtrate.
Preferably, the drying mode of the jasmine flowers and the calendula flowers is that freeze drying is firstly carried out and then microwave drying is carried out.
Wherein, the drying mode of jasmine flower is: rapidly freezing flos Jasmini sambac at-35deg.C for 1min, and freeze drying at-45deg.C under 18Pa for 1min; and then placing the freeze-dried jasmine flower into microwaves for drying, wherein the microwave power is 500W, and the microwave time is 5s.
The drying mode of the calendula is as follows: rapidly freezing herba Sidae Rhombifoliae at-20deg.C for 1min, and freeze-drying at-45deg.C under 18Pa for 0.5min; and then the calendula after freeze drying is dried by microwave with the microwave power of 550W and the microwave time of 10s.
Preferably, the drying mode of the camellia and the golden chamomile is microwave drying; the specific conditions are as follows: the microwave power is 550W and the microwave time is 10min.
Preferably, in S02, the components of the liquid medium are: 11.0g of casein peptone, 8.0g of beef extract, 5.0g of yeast powder, 5.0g of glucose, 5.0g of sodium acetate, 3.0g of acetic acid, 2.0g of diammonium citrate and 0.5g,Tween 80 1.0g,K g of citric acid 2 HPO 4 2.0g，MgSO 4 ·7H 2 O 0.2g，MnSO 4 ·H 2 O 0.05g，CaCO 3 20.0g, 15.0g of agar and 1.0L of distilled water.
Preferably, the inoculum size of the strain is 6-12wt%; particularly preferably 8wt%.
Preferably, the fermentation conditions are: introducing oxygen at 35+ -2deg.C, and fermenting for 48 hr.
In another aspect, the invention provides a jasmine flower composition fermentation filtrate, which is applied to the field of skin care products.
Preferably, the composition comprises any one or more of glycerol, erythritol, trehalose, glycerol glucoside, beta-glucan, tremella polysaccharide, 1, 2-hexanediol, 1, 2-pentanediol.
By adopting the technical scheme, the invention has the following beneficial effects:
the fermentation filtrate provided by the invention has excellent effects of relieving allergy, moisturizing, brightening, resisting wrinkles, resisting oxidization and maintaining beauty and keeping young, and the absorption of the fermentation filtrate is very strong. In addition, the general composition and cosmetics need to be added with preservative, such as carbopol or phenoxyethanol to improve the preservative performance, otherwise, the tank expansion condition occurs, the fermentation filtrate has better antibacterial capacity, the composition can be stored without adding the preservative, and the cosmetic with the composition can also not expand and grow bacteria under the condition of not adding the preservative.
According to the invention, four flowers are subjected to pre-drying treatment before fermentation, trace elements of fermentation filtrate obtained by subsequent fermentation are more abundant, and meanwhile, the research shows that the leaching rate is very low although effective components can be reserved to a great extent by adopting a freeze-drying mode for the pre-drying treatment; although the leaching rate is high by the microwave treatment, the effective components are partially damaged. The inventor researches and discovers that the two are combined, and the parameter is researched and discovers that the cell wall can be damaged; meanwhile, allergic reaction of part of specific people caused by sesquiterpene compounds in calendula can be greatly reduced; the damage to the effective components is reduced to the minimum; in addition, the method is found to be carried out, and after the culture medium is designed in the later fermentation process, the method is friendly to tea polysaccharide in extract, and meanwhile, two strains adopted in the invention are selected, the pH of fermentation filtrate is gradually reduced, and tea polysaccharide, jasmine flavone, catechin and the like are effectively obtained.
The invention is further illustrated with reference to specific embodiments.
Example 1
The embodiment provides a jasmine flower composition fermentation filtrate, wherein the jasmine flower composition comprises jasmine flowers, calendula, camellia, chamomile and strains;
the method for fermenting the filtrate by the jasmine flower composition comprises the following steps:
s01, respectively selecting jasmine, calendula, camellia and chamomile, cleaning, drying, crushing and sieving to obtain powder; the method comprises the steps of carrying out a first treatment on the surface of the The mass ratio of jasmine, calendula, camellia, golden chamomile is 3:85.7:5.9:31.3;
the drying mode of the jasmine flower is as follows: rapidly freezing flos Jasmini sambac at-35deg.C for 1min, and freeze drying at-45deg.C under 18Pa for 1min; and then placing the freeze-dried jasmine flower into microwaves for drying, wherein the microwave power is 500W, and the microwave time is 5s.
The drying mode of the calendula is as follows: rapidly freezing herba Sidae Rhombifoliae at-20deg.C for 1min, and freeze-drying at-45deg.C under 18Pa for 0.5min; and then the calendula after freeze drying is dried by microwave with the microwave power of 550W and the microwave time of 10s.
The camellia and golden chamomile are dried by microwaves; the specific conditions are as follows: the microwave power is 550W, and the microwave time is 10min.
S02, mixing the mixed powder obtained in the step S01 with a liquid culture medium; the mass ratio of the mixed powder to the liquid culture medium is 1:8; the liquid culture medium comprises the following components: 11.0g of casein peptone, 8.0g of beef extract, 5.0g of yeast powder, 5.0g of glucose, 5.0g of sodium acetate, 3.0g of acetic acid, 2.0g of diammonium citrate and 0.5g,Tween 80 1.0g,K g of citric acid 2 HPO 4 2.0g，MgSO 4 ·7H 2 O 0.2g，MnSO 4 ·H 2 O 0.05g，CaCO 3 20.0g, 15.0g of agar and 1.0L of distilled water.
S03, inoculating a strain into the mixed liquid culture medium obtained in the step S02 for fermentation, and then filtering to obtain jasmine flower composition fermentation filtrate; the inoculation amount of the strain is 8wt%, and the fermentation conditions are as follows: introducing oxygen at 35+ -2deg.C, and fermenting for 48 hr.
The strain is lactobacillus pentosus and lactococcus garvieae.
Lactobacillus pentosus Lactiplantibacillus pentosus is purchased from China industry microbiological culture Collection center, and the strain deposit number CICC 20301; lactococcus garvieae Lactococcus garvieae is purchased from China industry microbiological culture collection center, and the strain deposit number CICC is 21038; the inoculum size ratio was 2:1.
Example 2
The embodiment provides a jasmine flower composition fermentation filtrate, wherein the jasmine flower composition comprises jasmine flowers, calendula, camellia, chamomile and strains;
the method for fermenting the filtrate by the jasmine flower composition comprises the following steps:
s01, respectively selecting jasmine, calendula, camellia, golden chamomile, cleaning, crushing and sieving to obtain powder; the mass ratio of jasmine, calendula, camellia, golden chamomile is 3:85.7:5.9:31.3;
s02, mixing the mixed powder obtained in the step S01 with a liquid culture medium; the mass ratio of the mixed powder to the liquid culture medium is 1:8; the liquid culture medium comprises the following components: 11.0g of casein peptone, 8.0g of beef extract, 5.0g of yeast powder, 5.0g of glucose, 5.0g of sodium acetate, 3.0g of acetic acid, 2.0g of diammonium citrate and 0.5g,Tween 80 1.0g,K g of citric acid 2 HPO 4 2.0g，MgSO 4 ·7H 2 O 0.2g，MnSO 4 ·H 2 O 0.05g，CaCO 3 20.0g, 15.0g of agar and 1.0L of distilled water.
S03, inoculating a strain into the mixed liquid culture medium obtained in the step S02 for fermentation, and then filtering to obtain jasmine flower composition fermentation filtrate; the inoculation amount of the strain is 8wt%, and the fermentation conditions are as follows: introducing oxygen at 35+ -2deg.C, and fermenting for 48 hr.
The strain is lactobacillus pentosus and lactococcus garvieae.
Lactobacillus pentosus Lactiplantibacillus pentosus is purchased from China industry microbiological culture Collection center, and the strain deposit number CICC 20301; lactococcus garvieae Lactococcus garvieae is purchased from China industry microbiological culture collection center, and the strain deposit number CICC is 21038; the inoculum size ratio was 2:1.
Example 3
The embodiment provides a jasmine flower composition fermentation filtrate, wherein the jasmine flower composition comprises jasmine flowers, calendula, camellia, chamomile and strains;
the method for fermenting the filtrate by the jasmine flower composition comprises the following steps:
s01, respectively selecting jasmine, calendula, camellia and chamomile, cleaning, drying, crushing and sieving to obtain powder; the mass ratio of jasmine, calendula, camellia, golden chamomile is 3:85.7:5.9:31.3;
the drying mode of the jasmine, calendula, camellia, golden chamomile is microwave drying; the specific conditions are as follows: the microwave power is 550W, and the microwave time is 10min.
S02, mixing the mixed powder obtained in the step S01 with a liquid culture medium; the mass ratio of the mixed powder to the liquid culture medium is 1:8; the liquid culture medium comprises the following components: 11.0g of casein peptone, 8.0g of beef extract, 5.0g of yeast powder, 5.0g of glucose, 5.0g of sodium acetate, 3.0g of acetic acid, 2.0g of diammonium citrate and 0.5g,Tween 80 1.0g,K g of citric acid 2 HPO 4 2.0g，MgSO 4 ·7H 2 O 0.2g，MnSO 4 ·H 2 O 0.05g，CaCO 3 20.0g, 15.0g of agar and 1.0L of distilled water.
S03, inoculating a strain into the mixed liquid culture medium obtained in the step S02 for fermentation, and then filtering to obtain jasmine flower composition fermentation filtrate; the inoculation amount of the strain is 8wt%, and the fermentation conditions are as follows: introducing oxygen at 35+ -2deg.C, and fermenting for 48 hr.
The strain is lactobacillus pentosus and lactococcus garvieae.
Lactobacillus pentosus Lactiplantibacillus pentosus is purchased from China industry microbiological culture Collection center, and the strain deposit number CICC 20301; lactococcus garvieae Lactococcus garvieae is purchased from China industry microbiological culture collection center, and the strain deposit number CICC is 21038; the inoculum size ratio was 2:1.
Example 4
The embodiment provides a jasmine flower composition fermentation filtrate, wherein the jasmine flower composition comprises jasmine flowers, calendula, camellia, chamomile and strains;
the method for fermenting the filtrate by the jasmine flower composition comprises the following steps:
s01, respectively selecting jasmine, calendula, camellia and chamomile, cleaning, drying, crushing and sieving to obtain powder; the method comprises the steps of carrying out a first treatment on the surface of the The mass ratio of jasmine, calendula, camellia, golden chamomile is 3:85.7:5.9:31.3;
the drying mode of the jasmine flower is as follows: quick freezing flos Jasmini sambac at-35deg.C for 1min, and freeze drying at-45deg.C under 18Pa for 1min.
The drying mode of the calendula is as follows: rapidly freezing herba Sidae Rhombifoliae at-20deg.C for 1min, and freeze-drying at-45deg.C under 18Pa for 0.5min.
The camellia and golden chamomile are dried by microwaves; the specific conditions are as follows: the microwave power is 550W, and the microwave time is 10min.
S02, mixing the mixed powder obtained in the step S01 with a liquid culture medium; the mass ratio of the mixed powder to the liquid culture medium is 1:8; the liquid culture medium comprises the following components: 11.0g of casein peptone, 8.0g of beef extract, 5.0g of yeast powder, 5.0g of glucose, 5.0g of sodium acetate, 3.0g of acetic acid, 2.0g of diammonium citrate and 0.5g,Tween 80 1.0g,K g of citric acid 2 HPO 4 2.0g，MgSO 4 ·7H 2 O 0.2g，MnSO 4 ·H 2 O 0.05g，CaCO 3 20.0g, 15.0g of agar and 1.0L of distilled water.
S03, inoculating a strain into the mixed liquid culture medium obtained in the step S02 for fermentation, and then filtering to obtain jasmine flower composition fermentation filtrate; the inoculation amount of the strain is 8wt%, and the fermentation conditions are as follows: introducing oxygen at 35+ -2deg.C, and fermenting for 48 hr.
The strain is lactobacillus pentosus and lactococcus garvieae.
Lactobacillus pentosus Lactiplantibacillus pentosus is purchased from China industry microbiological culture Collection center, and the strain deposit number CICC 20301; lactococcus garvieae Lactococcus garvieae is purchased from China industry microbiological culture collection center, and the strain deposit number CICC is 21038; the inoculum size ratio was 2:1.
Example 5
The embodiment provides a jasmine flower composition fermentation filtrate, wherein the jasmine flower composition comprises jasmine flowers, calendula, camellia, chamomile and strains;
the method for fermenting the filtrate by the jasmine flower composition comprises the following steps:
s01, respectively selecting jasmine, calendula, camellia and chamomile, cleaning, drying, crushing and sieving to obtain powder; the method comprises the steps of carrying out a first treatment on the surface of the The mass ratio of jasmine, calendula, camellia, golden chamomile is 3:85.7:5.9:31.3;
the drying mode of the jasmine flower is as follows: rapidly freezing flos Jasmini sambac at-35deg.C for 1min, and freeze drying at-45deg.C under 18Pa for 1min; and then placing the freeze-dried jasmine flower into microwaves for drying, wherein the microwave power is 500W and 10s.
The drying mode of the calendula is as follows: rapidly freezing herba Sidae Rhombifoliae at-20deg.C for 1min, and freeze-drying at-45deg.C under 18Pa for 0.5min; and then the calendula after freeze drying is placed in microwave for drying, the microwave power is 550W, and the microwave time is 15s.
The camellia and golden chamomile are dried by microwaves; the specific conditions are as follows: the microwave power is 550W, and the microwave time is 10min.
S02, mixing the mixed powder obtained in the step S01 with a liquid culture medium; the mass ratio of the mixed powder to the liquid culture medium is 1:8; the liquid culture medium comprises the following components: 11.0g of casein peptone, 8.0g of beef extract, 5.0g of yeast powder, 5.0g of glucose, 5.0g of sodium acetate, 3.0g of acetic acid, 2.0g of diammonium citrate and 0.5g,Tween 80 1.0g,K g of citric acid 2 HPO 4 2.0g，MgSO 4 ·7H 2 O 0.2g，MnSO 4 ·H 2 O 0.05g，CaCO 3 20.0g, 15.0g of agar and 1.0L of distilled water.
S03, inoculating a strain into the mixed liquid culture medium obtained in the step S02 for fermentation, and then filtering to obtain jasmine flower composition fermentation filtrate; the inoculation amount of the strain is 8wt%, and the fermentation conditions are as follows: introducing oxygen at 35+ -2deg.C, and fermenting for 48 hr.
The strain is lactobacillus pentosus and lactococcus garvieae.
Lactobacillus pentosus Lactiplantibacillus pentosus is purchased from China industry microbiological culture Collection center, and the strain deposit number CICC 20301; lactococcus garvieae Lactococcus garvieae is purchased from China industry microbiological culture collection center, and the strain deposit number CICC is 21038; the inoculum size ratio was 2:1.
Example 6
The embodiment provides a jasmine flower composition fermentation filtrate, wherein the jasmine flower composition comprises jasmine flowers, calendula, camellia, chamomile and strains;
the method for fermenting the filtrate by the jasmine flower composition comprises the following steps:
s01, respectively selecting jasmine, calendula, camellia and chamomile, cleaning, drying, crushing and sieving to obtain powder; the method comprises the steps of carrying out a first treatment on the surface of the The mass ratio of jasmine, calendula, camellia, golden chamomile is 3:85.7:5.9:31.3;
the drying mode of the jasmine flower is as follows: rapidly freezing flos Jasmini sambac at-35deg.C for 1min, and freeze drying at-45deg.C under 18Pa for 1.5min; and then placing the freeze-dried jasmine flower into microwaves for drying, wherein the microwave power is 500W, and the microwave time is 5s.
The drying mode of the calendula is as follows: rapidly freezing herba Sidae Rhombifoliae at-20deg.C for 1min, and freeze-drying at-45deg.C under 18Pa for 1min; and then the calendula after freeze drying is dried by microwave with the microwave power of 550W and the microwave time of 10s.
The camellia and golden chamomile are dried by microwaves; the specific conditions are as follows: the microwave power is 550W, and the microwave time is 10min.
S02, mixing the mixed powder obtained in the step S01 with a liquid culture medium; the mass ratio of the mixed powder to the liquid culture medium is 1:8; the liquid culture medium comprises the following components: 11.0g of casein peptone, 8.0g of beef extract, 5.0g of yeast powder, 5.0g of glucose, 5.0g of sodium acetate, 3.0g of acetic acid, 2.0g of diammonium citrate and 0.5g,Tween 80 1.0g,K g of citric acid 2 HPO 4 2.0g，MgSO 4 ·7H 2 O 0.2g，MnSO 4 ·H 2 O 0.05g，CaCO 3 20.0g, 15.0g of agar and 1.0L of distilled water.
S03, inoculating a strain into the mixed liquid culture medium obtained in the step S02 for fermentation, and then filtering to obtain jasmine flower composition fermentation filtrate; the inoculation amount of the strain is 8wt%, and the fermentation conditions are as follows: introducing oxygen at 35+ -2deg.C, and fermenting for 48 hr.
The strain is lactobacillus pentosus and lactococcus garvieae.
Lactobacillus pentosus Lactiplantibacillus pentosus is purchased from China industry microbiological culture Collection center, and the strain deposit number CICC 20301; lactococcus garvieae Lactococcus garvieae is purchased from China industry microbiological culture collection center, and the strain deposit number CICC is 21038; the inoculum size ratio was 2:1.
Example 7
The embodiment provides a jasmine flower composition fermentation filtrate, wherein the jasmine flower composition comprises jasmine flowers, calendula, camellia, chamomile and strains;
the method for fermenting the filtrate by the jasmine flower composition comprises the following steps:
s01, respectively selecting jasmine, calendula, camellia and chamomile, cleaning, drying, crushing and sieving to obtain powder; the method comprises the steps of carrying out a first treatment on the surface of the The mass ratio of jasmine, calendula, camellia, golden chamomile is 3:85.7:5.9:31.3;
the drying mode of the jasmine flower is as follows: rapidly freezing flos Jasmini sambac at-35deg.C for 1min, and freeze drying at-45deg.C under 18Pa for 1min; and then placing the freeze-dried jasmine flower into microwaves for drying, wherein the microwave power is 500W, and the microwave time is 5s.
The drying mode of the calendula is as follows: rapidly freezing herba Sidae Rhombifoliae at-20deg.C for 1min, and freeze-drying at-45deg.C under 18Pa for 0.5min; and then the calendula after freeze drying is dried by microwave with the microwave power of 550W and the microwave time of 10s.
The camellia and golden chamomile are dried by microwaves; the specific conditions are as follows: the microwave power is 550W, and the microwave time is 10min.
S02, mixing the mixed powder obtained in the step S01 with a liquid culture medium; the mass ratio of the mixed powder to the liquid culture medium is 1:8; the liquid culture medium comprises the following components: 11.0g of casein peptone, 8.0g of beef extract, 5.0g of yeast powder, 5.0g of glucose, 5.0g of sodium acetate, 3.0g of acetic acid, 2.0g of diammonium citrate and 0.5g,Tween 80 1.0g,K g of citric acid 2 HPO 4 2.0g，MgSO 4 ·7H 2 O 0.2g，MnSO 4 ·H 2 O 0.05g，CaCO 3 20.0g, 15.0g of agar and 1.0L of distilled water.
S03, inoculating a strain into the mixed liquid culture medium obtained in the step S02 for fermentation, and then filtering to obtain jasmine flower composition fermentation filtrate; the inoculation amount of the strain is 8wt%, and the fermentation conditions are as follows: introducing oxygen at 35+ -2deg.C, and fermenting for 48 hr.
The strain is lactobacillus pentosus and lactococcus garvieae.
Lactobacillus pentosus Lactiplantibacillus pentosus is purchased from China industry microbiological culture Collection center, and the strain deposit number CICC 20301; lactococcus garvieae Lactococcus garvieae is purchased from China industry microbiological culture collection center, and the strain deposit number CICC is 21038; the inoculum size ratio was 2:1.
Example 8
The embodiment provides a jasmine flower composition fermentation filtrate, wherein the jasmine flower composition comprises jasmine flowers, calendula, camellia, chamomile and strains;
the method for fermenting the filtrate by the jasmine flower composition comprises the following steps:
s01, respectively selecting jasmine, calendula, camellia and chamomile, cleaning, drying, crushing and sieving to obtain powder; the mass ratio of jasmine, calendula, camellia, golden chamomile is 3:85.7:5.9:31.3;
the drying mode of the jasmine flower is as follows: rapidly freezing flos Jasmini sambac at-35deg.C for 1min, and freeze drying at-45deg.C under 18Pa for 1min; and then placing the freeze-dried jasmine flower into microwaves for drying, wherein the microwave power is 500W and 5s.
The drying mode of the calendula is as follows: rapidly freezing herba Sidae Rhombifoliae at-20deg.C for 1min, and freeze-drying at-45deg.C under 18Pa for 0.5min; and then the calendula after freeze drying is dried by microwave with the microwave power of 550W and the microwave time of 10s.
The camellia and golden chamomile are dried by microwaves; the specific conditions are as follows: the microwave power is 550W, and the microwave time is 10min.
S02, mixing the mixed powder obtained in the step S01 with a liquid culture medium; the mass ratio of the mixed powder to the liquid culture medium is 1:8; the liquid culture medium comprises the following components: 11.0g of casein peptone, which is used for treating the skin,8.0g of beef extract, 5.0g of yeast powder, 5.0g of glucose, 5.0g of sodium acetate, 3.0g of acetic acid, 2.0g of diammonium citrate and 0.5g,Tween 80 1.0g,K g of citric acid 2 HPO 4 2.0g，MgSO 4 ·7H 2 O 0.2g，MnSO 4 ·H 2 O 0.05g，CaCO 3 20.0g, 15.0g of agar and 1.0L of distilled water.
S03, inoculating a strain into the mixed liquid culture medium obtained in the step S02 for fermentation, and then filtering to obtain jasmine flower composition fermentation filtrate; the inoculation amount of the strain is 8wt%, and the fermentation conditions are as follows: introducing oxygen at 35+ -2deg.C, and fermenting for 48 hr.
The strain is lactobacillus pentosus and lactococcus garvieae.
Lactobacillus pentosus Lactiplantibacillus pentosus is purchased from China industry microbiological culture Collection center, and the strain deposit number CICC 20301; lactococcus garvieae Lactococcus garvieae is purchased from China industry microbiological culture collection center, and the strain deposit number CICC is 21038; the inoculum size ratio was 2:1.
Example 9
The embodiment provides a jasmine flower composition fermentation filtrate, wherein the jasmine flower composition comprises jasmine flowers, calendula, camellia, chamomile and strains;
the method for fermenting the filtrate by the jasmine flower composition comprises the following steps:
s01, respectively selecting jasmine, calendula, camellia and chamomile, cleaning, drying, crushing and sieving to obtain powder; the method comprises the steps of carrying out a first treatment on the surface of the The mass ratio of jasmine, calendula, camellia, golden chamomile is 3:85.7:5.9:31.3;
the drying mode of the jasmine flower is as follows: rapidly freezing flos Jasmini sambac at-35deg.C for 1min, and freeze drying at-45deg.C under 18Pa for 1min; and then placing the freeze-dried jasmine flower into microwaves for drying, wherein the microwave power is 500W, and the microwave time is 5s.
The drying mode of the calendula is as follows: rapidly freezing herba Sidae Rhombifoliae at-20deg.C for 1min, and freeze-drying at-45deg.C under 18Pa for 0.5min; and then the calendula after freeze drying is dried by microwave with the microwave power of 550W and the microwave time of 10s.
The camellia and golden chamomile are dried by microwaves; the specific conditions are as follows: the microwave power is 550W, and the microwave time is 10min.
S02, mixing the mixed powder obtained in the step S01 with a liquid culture medium; the mass ratio of the mixed powder to the liquid culture medium is 1:8; the liquid culture medium comprises the following components: 11.0g of casein peptone, 8.0g of beef extract, 5.0g of yeast powder, 5.0g of glucose, 5.0g of sodium acetate, 3.0g of acetic acid, 2.0g of diammonium citrate and 0.5g,Tween 80 1.0g,K g of citric acid 2 HPO 4 2.0g，MgSO 4 ·7H 2 O 0.2g，MnSO 4 ·H 2 O 0.05g，CaCO 3 20.0g, 15.0g of agar and 1.0L of distilled water.
S03, inoculating a strain into the mixed liquid culture medium obtained in the step S02 for fermentation, and then filtering to obtain jasmine flower composition fermentation filtrate; the inoculation amount of the strain is 8wt%, and the fermentation conditions are as follows: introducing oxygen at 35+ -2deg.C, and fermenting for 48 hr.
The strain is lactobacillus pentosus.
Lactobacillus pentosus Lactiplantibacillus pentosus is purchased from China industry microbiological culture Collection center, and the strain deposit number CICC 20301.
Example 10
The embodiment provides a jasmine flower composition fermentation filtrate, wherein the jasmine flower composition comprises jasmine flowers, calendula, camellia, chamomile and strains;
the method for fermenting the filtrate by the jasmine flower composition comprises the following steps:
s01, respectively selecting jasmine, calendula, camellia and chamomile, cleaning, drying, crushing and sieving to obtain powder; the method comprises the steps of carrying out a first treatment on the surface of the The mass ratio of jasmine, calendula, camellia, golden chamomile is 3:85.7:5.9:31.3;
the drying mode of the jasmine flower is as follows: rapidly freezing flos Jasmini sambac at-35deg.C for 1min, and freeze drying at-45deg.C under 18Pa for 1min; and then placing the freeze-dried jasmine flower into microwaves for drying, wherein the microwave power is 500W, and the microwave time is 5s.
The drying mode of the calendula is as follows: rapidly freezing herba Sidae Rhombifoliae at-20deg.C for 1min, and freeze-drying at-45deg.C under 18Pa for 0.5min; and then the calendula after freeze drying is dried by microwave with the microwave power of 550W and the microwave time of 10s.
The camellia and golden chamomile are dried by microwaves; the specific conditions are as follows: the microwave power is 550W, and the microwave time is 10min.
S02, mixing the mixed powder obtained in the step S01 with a liquid culture medium; the mass ratio of the mixed powder to the liquid culture medium is 1:8; the liquid culture medium comprises the following components: 11.0g of casein peptone, 8.0g of beef extract, 5.0g of yeast powder, 5.0g of glucose, 5.0g of sodium acetate, 3.0g of acetic acid, 2.0g of diammonium citrate and 0.5g,Tween 80 1.0g,K g of citric acid 2 HPO 4 2.0g，MgSO 4 ·7H 2 O 0.2g，MnSO 4 ·H 2 O 0.05g，CaCO 3 20.0g, 15.0g of agar and 1.0L of distilled water.
S03, inoculating a strain into the mixed liquid culture medium obtained in the step S02 for fermentation, and then filtering to obtain jasmine flower composition fermentation filtrate; the inoculation amount of the strain is 8wt%, and the fermentation conditions are as follows: introducing oxygen at 35+ -2deg.C, and fermenting for 48 hr.
The strain is lactococcus garvieae.
Lactococcus garvieae Lactococcus garvieae is purchased from China industry microbiological culture Collection center, with strain deposit number CICC cube 21038.
Example 11
The embodiment provides a jasmine flower composition fermentation filtrate, wherein the jasmine flower composition comprises jasmine flowers, calendula, camellia, chamomile and strains;
the method for fermenting the filtrate by the jasmine flower composition comprises the following steps:
s01, respectively selecting jasmine, calendula, camellia and chamomile, cleaning, drying, crushing and sieving to obtain powder; the method comprises the steps of carrying out a first treatment on the surface of the The mass ratio of jasmine, calendula, camellia, golden chamomile is 3:85.7:5.9:31.3;
the drying mode of the jasmine flower is as follows: rapidly freezing flos Jasmini sambac at-35deg.C for 1min, and freeze drying at-45deg.C under 18Pa for 1min; and then placing the freeze-dried jasmine flower into microwaves for drying, wherein the microwave power is 500W, and the microwave time is 5s.
The drying mode of the calendula is as follows: rapidly freezing herba Sidae Rhombifoliae at-20deg.C for 1min, and freeze-drying at-45deg.C under 18Pa for 0.5min; and then the calendula after freeze drying is dried by microwave with the microwave power of 550W and the microwave time of 10s.
The camellia and golden chamomile are dried by microwaves; the specific conditions are as follows: the microwave power is 550W, and the microwave time is 10min.
S02, mixing the mixed powder obtained in the step S01 with a liquid culture medium; the mass ratio of the mixed powder to the liquid culture medium is 1:8; the liquid culture medium comprises the following components: 11.0g of casein peptone, 8.0g of beef extract, 5.0g of yeast powder, 5.0g of glucose, 5.0g of sodium acetate, 3.0g of acetic acid, 2.0g of diammonium citrate and 0.5g,Tween 80 1.0g,K g of citric acid 2 HPO 4 2.0g，MgSO 4 ·7H 2 O 0.2g，MnSO 4 ·H 2 O 0.05g，CaCO 3 20.0g, 15.0g of agar and 1.0L of distilled water.
S03, inoculating a strain into the mixed liquid culture medium obtained in the step S02 for fermentation, and then filtering to obtain jasmine flower composition fermentation filtrate; the inoculation amount of the strain is 8wt%, and the fermentation conditions are as follows: introducing oxygen at 35+ -2deg.C, and fermenting for 48 hr.
The strain is Lactobacillus pentosus and Bifidobacterium adolescentis.
Lactobacillus pentosus Lactiplantibacillus pentosus is purchased from China industry microbiological culture Collection center, and the strain deposit number CICC 20301; bifidobacterium adolescentis Bifidobacterium adolensentis is purchased from China center for type culture Collection of industrial microorganisms, and the strain deposit number is CICC 6179; the inoculum size ratio was 2:1.
Performance testing
Test 1: hyaluronidase inhibition assay
The detection basis is tested according to the inhibition rate of HMC-W1-029 hyaluronidase.
Examples 1 to 11: diluting with pure water to a sample concentration of 0.2%;
positive control (dipotassium glycyrrhizinate with purity not less than 98%): diluting with water to 3% positive control concentration;
negative control: pure water.
The test operation steps are as follows:
the example group, the positive control group and the negative control group are designed, 3 parallels are required to be set up for each group, different reagent solutions are respectively added, the mixture is uniformly shaken, the mixture is placed at room temperature for 30min for color development, and an absorbance value with the wavelength of 528nm is measured by an ultraviolet spectrophotometer.
Calculation formula
Hyaluronidase inhibition ratio (%) = (1- (C-D)/(a-B)) ×100
Wherein A is absorbance of the reaction solution without the sample; b is the absorbance of the reaction solution without sample and enzyme; c is the absorbance of the reaction solution containing the sample and the enzyme; d is the absorbance of the reaction solution containing the sample and containing no enzyme.
The statistical analysis software is SPSS, and the comparison among the hyaluronidase inhibition rates of the test sample, the positive control substance and the negative control substance adopts independent template t test. The statistical analysis is a two-tailed test, the significance level is alpha=0.05, and p > 0.05, which indicates that no significance difference exists between the two groups; p < 0.05, indicating a significant difference between the two groups.
Table 1 test results table
| Name of the name | Experimental results | P value |
| Example 1 | 73.311±0.176 | ＜0.05 |
| Example 2 | 29.136±0.267 | ＜0.05 |
| Example 3 | 56.142±0.251 | ＜0.05 |
| Example 4 | 59.689±0.418 | ＜0.05 |
| Example 5 | 68.496±0.263 | ＜0.05 |
| Example 6 | 67.413±0.431 | ＜0.05 |
| Example 7 | 54.328±0.265 | ＜0.05 |
| Example 8 | 53.311±0.471 | ＜0.05 |
| Example 9 | 49.251±0.479 | ＜0.05 |
| Implementation of the embodimentsExample 10 | 51.111±0.116 | ＜0.05 |
| Example 11 | 33.322±0.167 | ＜0.05 |
| Negative control | -4.965±0.259 | / |
| Positive control | 51.239±1.314 | ＜0.05 |
The hyaluronidase inhibition rate remained three decimal places, P < 0.05, indicating that there was a significant difference between the sample and the positive control compared to the negative control.
Test 2 elastase inhibition Rate
Fermentation filtrate: diluting with pure water until the concentration of the sample is 5%;
positive control (epigallocatechin gallate): diluting with water to a positive control concentration of 0.1%;
negative control: pure water.
The test operation steps are as follows:
designing a sample group, a sample background group, a solvent group and a solvent background group, setting 3 groups in parallel, respectively adding different reagent solutions into a 96-well plate, gently shaking, incubating at 25 ℃ for 15min, placing the sample groups in an enzyme-labeled instrument, and measuring the absorbance at 410 nm.
The calculation formula is as follows: elastase inhibition ratio (%) = (1- (C-D)/(a-B)) ×100
Wherein A is absorbance of the reaction solution without the sample; b is the absorbance of the reaction solution without sample and enzyme; c is the absorbance of the reaction solution containing the sample and the enzyme; d is the absorbance of the reaction solution containing the sample and containing no enzyme.
The statistical analysis software is SPSS, and the comparison among the elastase inhibition rates of the test sample, the positive control and the negative control adopts independent template t test. The statistical analysis is a two-tailed test, the significance level is alpha=0.05, and p > 0.05, which indicates that no significance difference exists between the two groups; p < 0.05, indicating a significant difference between the two groups.
Table 2 test results table
The elastase inhibition rate remained three decimal places, P < 0.05, indicating a significant difference between the sample and the positive control compared to the negative control.
Test 3: fragrance retention test for resident products
5g of each of the products of examples 1-6 are respectively taken and then evenly mixed with 95g of clear water to obtain a mixed solution for standby. Taking 18 PMMA sheets, dividing the PMMA sheets into 6 groups of 3 sheets, and marking; 1g of the mixed solution was dropped on one PMMA plate for each group, and 1g of deionized water was dropped on the other 2 plates of each group. Subsequently, the mixture was placed in a constant temperature and humidity [ (21.+ -. 1). ] C, (50.+ -. 10)% RH ] environment for 24 hours, and fragrance-smelling tests were performed on 6 groups of PMMA tablets by 5 evaluators.
Table 3 fragrance retention test results
Note that filling "1" with fragrance and filling "0" without fragrance "
Test 4: the market randomly selects the population of 25-35 years old, dry sensitive skin, 70 people total, randomly divided into 7 groups, the first 6 groups are respectively diluted to 5% concentration with deionized water using examples 1-6; deionized water was used for group 7. The skin allergy after use is checked by using the medicine twice a day in 8:00 a day and 20:00 a night for 30 days continuously. While increasing the questionnaire.
Problem one:
whether you like the present fragrance
3 points very like 2 points like 1 point and 0 point generally dislike
And a second problem: whether you feel this fragrance more pleasant
A score of 3 is very pleasant 2 and a score of 1 is generally unpleasant 0
Score statistics: the scores of each group were summed to give a total score which was filled into table 4.
Table 4 results of test 4
| Examples | Skin condition | Questionnaire (question one) | Questionnaire (question two) |
| Group 1 | Has no allergy, redness, etc | 30 | 30 |
| Group 2 | Acne-making | 23 | 23 |
| Group 3 | A small part of reddening | 24 | 24 |
| Group 4 | A small part of reddening | 23 | 23 |
| Group 5 | A small part of reddening | 25 | 24 |
| Group 6 | A small part of reddening | 25 | 25 |
| Group 7 | Dry, allergic, reddish and red blood streaks | - | - |
Test 5, test of anti-corrosive Property
Taking 20 culture dishes, randomly and averagely dividing the culture dishes into 4 groups, loading 18g of mask mud without antiseptic components and containing Chinese medicinal powder into 1 group, loading 17.5g of mask mud without antiseptic components and containing Chinese medicinal powder and 0.5g of fermentation filtrate of the embodiment 1 into 2 groups, loading 17g of mask mud without antiseptic components and containing Chinese medicinal powder and 1g of fermentation filtrate of the embodiment 1 into 3 groups, loading 18g of fermentation filtrate of the embodiment 1 into 4 groups, and placing the 20 culture dishes into a 25-DEG incubator to observe colony growth conditions, wherein the Chinese medicinal powder is ginseng powder in the test.
Table 5 results of test 5
The 0 level represents the non-living colony, the 5 level represents the serious colony, and the 0-5 level represents the more serious colony in turn.
Finally, it should be noted that: the above embodiments are only for illustrating the technical solution of the present invention, and not for limiting the same; although the invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical scheme described in the foregoing embodiments can be modified or some or all of the technical features thereof can be replaced by equivalents; such modifications and substitutions do not depart from the spirit of the invention.
Claims (9)
1. The jasmine flower composition fermented filtrate is characterized by comprising the following preparation raw materials: jasmine, calendula, camellia, chamomile and bacterial species;
the strain is lactobacillus pentosus and lactococcus garvieae;
the drying mode of the jasmine flower is as follows: rapidly freezing flos Jasmini sambac at-35deg.C for 1min, and freeze drying at-45deg.C under 18Pa for 1min; and then placing the freeze-dried jasmine flower into microwaves for drying, wherein the microwave power is 500W, and the microwave time is 5s.
2. A method for preparing the jasmine flower composition fermentation filtrate of claim 1, which comprises the following steps:
s01, respectively selecting jasmine, calendula, camellia and chamomile, cleaning, drying, crushing and sieving to obtain powder;
s02, mixing the powder obtained in the step S01 with a liquid culture medium;
s03, inoculating a strain into the mixed liquid culture medium obtained in the step S02 for fermentation, and then filtering to obtain a jasmine flower composition fermentation filtrate.
3. The method for preparing a fermentation filtrate of a jasmine composition according to claim 2, wherein the calendula is dried by freeze-drying followed by microwave drying.
4. The method for preparing a fermentation filtrate of a jasmine composition according to claim 2, wherein the drying mode of the camellia and golden chamomile is microwave drying.
5. The method for preparing a fermentation filtrate of a jasmine composition according to claim 2, wherein in S02, the components of the liquid medium are: 11.0g of casein peptone, 8.0g of beef extract, 5.0g of yeast powder, 5.0g of glucose, 5.0g of sodium acetate, 3.0g of acetic acid, 2.0g of diammonium citrate and 0.5g,Tween 80 1.0g,K g of citric acid 2 HPO 4 2.0g，MgSO 4 ·7H 2 O 0.2g，MnSO 4 ·H 2 O 0.05g，CaCO 3 20.0g, 15.0g of agar and 1.0L of distilled water.
6. The method for preparing a fermentation filtrate of a jasmine composition according to claim 2, wherein the seed amount of the seed culture in S03 is 6-12wt%.
7. The method of preparing a jasmine composition fermentation filtrate of claim 2, wherein the fermentation conditions are: introducing oxygen at 35+ -2deg.C, and fermenting for 48 hr.
8. The jasmine composition fermentation filtrate of claim 1, further comprising any one or more of glycerol, erythritol, trehalose, glycerol glucoside, beta-glucan, tremella polysaccharide, 1, 2-hexanediol, 1, 2-pentanediol.
9. A jasmine composition fermented filtrate as claimed in claim 1, which is used in the field of skin care products.
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