CN116549328A - Use of peach gum polysaccharide in products for promoting tissue repair or improving aesthetic skin - Google Patents
Use of peach gum polysaccharide in products for promoting tissue repair or improving aesthetic skin Download PDFInfo
- Publication number
- CN116549328A CN116549328A CN202310725466.7A CN202310725466A CN116549328A CN 116549328 A CN116549328 A CN 116549328A CN 202310725466 A CN202310725466 A CN 202310725466A CN 116549328 A CN116549328 A CN 116549328A
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- China
- Prior art keywords
- peach gum
- polysaccharide
- gum polysaccharide
- phase
- skin
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Classifications
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/16—Emollients or protectives, e.g. against radiation
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/005—Preparations for sensitive skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/008—Preparations for oily skin
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Birds (AREA)
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- Polymers & Plastics (AREA)
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Abstract
The invention belongs to the technical field of cosmetics, and particularly relates to application of peach gum polysaccharide in products for promoting tissue repair or improving skin aesthetic feeling. The invention provides application of peach gum polysaccharide in products for promoting tissue repair or improving skin aesthetic feeling, and experiments prove that the peach gum polysaccharide provided by the invention has potential wound healing and skin injury repairing effects under the condition of not affecting cell proliferation and cell morphology, and has better anti-inflammatory and gap closing promoting effects at different concentrations, thereby playing a role in improving skin aesthetic feeling.
Description
Technical Field
The invention belongs to the technical field of cosmetics, and particularly relates to application of peach gum polysaccharide in products for promoting tissue repair or improving skin aesthetic feeling.
Background
Skin serves as the largest organ of the human body and has many important functions such as sensing the environment, regulating body temperature, providing passive and active defenses, etc. However, when the extent of skin damage exceeds its own repairability, it is often necessary to promote wound healing by means of exogenous intervention, such as bandaging, patch application, and the like. The wound healing process mainly comprises 4 stages of coagulation, inflammation, proliferation and remodeling, and any disorder in the healing process can delay wound healing, worsen the wound, even become chronic wounds, increase pain of patients, and continuously increase later morbidity and treatment cost. Therefore, it is important to find and develop drugs that promote wound healing.
Peach gum is also called peach tear, peach oil and peach tree gum, and is a colloidal exudate of damaged trunk or fungal infection of plants of Rosaceae such as peach, and has medicinal and edible value. The peach gum is used as a traditional medicine and food and has food materials, accords with the modern concept of green development, has wide application prospect, can be directly eaten, can be used as an additive and a food preservative, and is applied to industries such as food, medicine, cosmetics and the like, wherein the novel embedding material, the skin care raw material and the like. Peach gum has medicinal value, tangsu May be "Tang Ben Cao" (the official 659 years) is: for stranguria due to urinary calculus, blood stasis, it is indicated for aversion to the middle energizer . According to the cloud of "Baozi" (Jin Daige flood): peach gum is taken after Sang Huizhi, and can remove hundred diseases, break grains for several months, and make the tarnish night appear as a month after a long time, and the prescription is "deficiency heat is thirsty: peach gum is large like a bolus and contains good ingredients. The peach gum has the main effects of inducing diuresis for treating stranguria, and is used for treating urolithiasis, stranguria with blood and dysentery. The researches show that the peach gum has the effects and functions of removing stones, protecting hematopoiesis, prolonging life, retaining youthful looks and the like.
The main component and the functional component of the peach gum are polysaccharide, and the peach gum has good application prospect in the fields of food, medicine, cosmetics and the like, such as physical and chemical properties, biological activity and the like. In recent years, peach gum polysaccharide has a plurality of pharmacological effects: moisturizing, moisture absorption, antioxidation, bowel relaxing, antibiosis, blood sugar reduction, blood fat reduction, immunity improvement, intestinal probiotics growth promotion and the like, but the peach gum is not deeply researched at present, the efficacy is not perfect, and a plurality of active functions are not developed yet.
Chinese patent document CN111214483A discloses an application of peach gum polysaccharide, in particular to an application of peach gum polysaccharide in resisting allergy, relieving itching, diminishing inflammation and relieving pain to skin; chinese patent document CN108992459A discloses application of peach gum polysaccharide in preparing medicament for treating or preventing nephritis and a pharmaceutical composition, and in particular discloses application of peach gum polysaccharide in improving biochemical index of nephritis, improving immunity, relieving verification reaction, recovering vitality of kidney corpuscle and having certain antibacterial effect.
In view of the above, at present, no article has been made to indicate that peach gum polysaccharide has a promoting effect on wound healing, and no patent application has yet been filed. Therefore, aiming at the problem of insufficient development and utilization of peach gum, the nutrition value and the medicinal value of the peach gum polysaccharide are deeply analyzed, which is helpful for the redevelopment and application of the peach gum, and lays a foundation for the development and application of the peach gum, thereby having important significance.
Disclosure of Invention
Aiming at the defects of the prior art, the invention further researches and develops peach gum, and aims to provide the application of peach gum polysaccharide in products for promoting tissue repair or improving skin aesthetic feeling.
In order to achieve the above purpose, the present invention adopts the following technical scheme:
it is a first object of the present invention to provide the use of peach gum polysaccharide in products that promote tissue repair or improve the aesthetic appearance of skin.
Preferably, the product is a pharmaceutical or cosmetic product.
Preferably, the pharmaceutical or cosmetic dosage form includes a gel paste, an ointment, a topical liniment, a patch, a cream, an emulsion, a gel or a toner.
Preferably, the preparation method of the peach gum polysaccharide comprises the following steps:
(1) Soaking and swelling: adding pure water into the peach gum, uniformly stirring, soaking the peach gum until the peach gum is completely swelled after the peach gum is completely wetted, and cleaning impurities; the feed liquid ratio of the peach gum to the pure water is 1 g:25-50 mL; the swelling time is 4-12 h;
(2) Heating and decocting: adding pure water into the peach gum cleaned in the step (1), and heating and decocting to obtain a feed liquid 1; the feed liquid ratio of the peach gum to the pure water is 1 g:50-100 mL; the decoction temperature is 60-80 ℃ and the decoction time is 1.5-2 h;
(3) Homogenizing under high pressure: heating the step (2) to obtain feed liquid 1, and homogenizing under high pressure to completely dissolve and disperse peach gum to obtain feed liquid 2; the homogenizing pressure is 60-100 Bar, the homogenizing temperature is 121-150 ℃, and the homogenizing time is 0.5-2.5 h;
(4) And (3) suction filtration: carrying out suction filtration on the feed liquid 2 obtained in the step (3) to remove insoluble impurities, thereby obtaining polysaccharide extract;
(5) Ultrafiltration and freeze-drying: and (3) carrying out ultrafiltration concentration on the polysaccharide extract obtained in the step (4), collecting peach gum polysaccharide with molecular weight more than 3kDa, and freeze-drying to obtain the peach gum polysaccharide.
Preferably, the addition amount of the peach gum polysaccharide in the product is 0.5-5 w%.
Another object of the present invention is to provide a cosmetic containing peach gum polysaccharide, comprising, by weight, 0.05-0.2% of skin feel modifier, 0.2-1.2% of emulsifier, 4-8% of thickener, 8-20% of humectant, 0.5-2.5% of emollient, 0.05-0.6% of preservative, 0.01-0.1% of colorant, 0.01-0.1% of essence, 0.5-2.0% of peach gum polysaccharide and the balance of water.
Preferably, the skin feel modifier is ethylhexyl glycerol;
the emulsifier is one or more of stearyl alcohol polyether-2, oleyl alcohol polyether-10, methoxy PEG/PPG-25/4 polydimethylsiloxane and PEG-10 polydimethylsiloxane;
The thickener comprises one or more of alginic acid and its salt, pectin, sodium hyaluronate, cetyl alcohol polyoxyethylene (3) ether, sodium chloride, potassium chloride, p-hydroxyacetophenone, and cyclopenta-dimethicone;
the humectant comprises one or more of sorbitol, glycerol, propylene glycol, butanediol, xylitol, sodium lactate, hyaluronic acid, pullulan, ceramide and triglyceride;
the softening agent comprises one or more of C10-18 fatty acid triglyceride, caprylic acid triglyceride, dimethyl silicone oil, isopropyl lanolate, acetylated lanonol, isopropyl myristate, cetyl alcohol and stearyl alcohol;
the preservative comprises one or more of benzethonium chloride and p-hydroxyacetophenone;
the colorant is gardenia blue pigment.
Preferably, the method comprises the following steps:
(1) Preparing a solution: weighing emulsifier, thickener, softener and essence according to the formula amount, and uniformly stirring to prepare phase A, wherein the phase A is an oil phase for later use; weighing humectant, peach gum polysaccharide and water according to the formula amount, stirring and mixing uniformly to prepare a phase B for later use; weighing a thickening agent, a preservative, a colorant and a skin feel regulator according to the formula amount, and uniformly stirring and mixing to prepare a C phase, wherein the C phase is a water phase for later use;
(2) Adding phase B into phase C, heating, stirring for dissolving, slowly pouring the dissolved mixed raw material into phase A, and stirring uniformly;
(3) Stopping stirring to obtain cosmetic containing peach gum polysaccharide.
Compared with the prior art, the invention has the following beneficial effects:
1. the invention provides a new application of peach gum polysaccharide in products for promoting tissue repair or improving skin aesthetic feeling, and experiments prove that the peach gum polysaccharide has better effect of promoting gap closure, and further experiments prove that the peach gum polysaccharide has potential wound healing under the condition of not affecting cell proliferation and cell morphology, has the effects of repairing skin injury and improving skin aesthetic feeling, and is beneficial to redevelopment and application of the peach gum.
2. The average molecular weight of the peach gum polysaccharide prepared by the invention is 55-65kDa, which is reduced compared with the results reported in the literature, and the high performance liquid chromatography of the peach gum polysaccharide is unimodal, so that the solubility and the biological activity of the peach gum polysaccharide are improved. Therefore, the peach gum polysaccharide provided by the invention has low molecular weight, good solubility and a shear thinning effect, and can be applied to a viscosity regulator to effectively improve the viscosity and the fluidity of a product.
3. The invention also provides a cosmetic containing the peach gum polysaccharide, which has good transparency, moisture retention, gloss and smoothness, better refreshing degree and compactness, good oil control effect, and can improve the effect of sensitive skin to a certain extent on the basis of shrinking compact pores.
Drawings
FIG. 1 is a graph showing experimental results of the effect of different concentrations of peach gum polysaccharide on wound healing;
FIG. 2 is a graph showing experimental results of the effect of the anti-inflammatory activity of peach gum polysaccharide;
FIG. 3 is a graph showing analysis results of the composition of the peach gum polysaccharide monosaccharides, wherein FIG. 3 (A) shows a gas chromatogram of the peach gum polysaccharide prepared by the invention, and FIG. 3 (B) shows the mole percentage of the composition of the peach gum monosaccharides;
FIG. 4 is a graph showing rheological properties of peach gum polysaccharide prepared by the invention in solutions with different concentrations, wherein FIG. 4 (A) is a graph showing changes of shear stress with shear rate, and FIG. 4 (B) is a graph showing changes of viscosity with shear rate;
FIG. 5 is a graph showing the appearance and cold and heat resistance experimental results of the peach gum polysaccharide water-out cream product;
FIG. 6 is a graph showing static rheological property experiments of peach gum polysaccharide water bloom, wherein FIG. 6 (A) is a graph showing the change of viscosity with the shear rate, and FIG. 6 (B) is a graph showing the change of shear stress with the shear rate;
FIG. 7 is a graph showing dynamic rheological characteristics of peach gum polysaccharide water bloom, wherein FIG. 7 (A) is a graph showing elasticity and viscosity as a function of shear frequency, and FIG. 7 (B) is a graph showing elasticity performance as a function of shear frequency;
FIG. 8 is a graph showing the results of skin sensory evaluation of peach gum polysaccharide water bloom according to the present invention;
Fig. 9 is a graph of the evaluation result of the effect of peach gum polysaccharide water bloom.
Detailed Description
The above-described aspects of the present invention will be described in further detail with reference to the following embodiments. It should not be construed that the scope of the above subject matter of the present invention is limited to the following examples.
Example 1 cell culture
(1) Cell resuscitation
Complete medium: DMEM+10% foetal calf serum+1% penicillin-streptomycin (10000U/mL)
The water bath was preheated to 37 ℃. Taking out the frozen cells from the liquid nitrogen, slightly shaking the frozen cells in a water bath at 37 ℃ to enable the liquid in the frozen cells to be melted quickly, transferring the liquid in the frozen cells to a sterile centrifuge tube containing 3mL of complete culture medium quickly, adding the culture medium to 10mL, slightly mixing the culture medium, and centrifuging at 1000rpm for 5min. Carefully blotting the supernatant, resuspending the cells with 1-2mL of a gentle blow bottom pellet, transferring the cell suspension to a cell culture flask containing appropriate amount of medium, and placing at 37deg.C, 5% CO 2 Culturing in an incubator.
(2) Cell passage
The cells in the culture flask were observed and spread over 80% for passaging. The cell morphology was observed and compared with the image on ATCC, and the cells in good condition were frozen for seed preservation. The cell culture medium, 0.25% pancreatin and PBS were all pre-warmed at 37 ℃. The old culture broth was aspirated, cells were washed with an appropriate amount of PBS, 1mL of 0.25% pancreatin was added to cover the bottom of the flask after the PBS was aspirated, and the flask was allowed to stand at 37℃for digestion. The digestion was observed under a microscope, at which time most of the cells gradually contracted and rounded and detached from the bottom of the flask, and a small portion of the cells, while still adherent, were rapidly added with 2 volumes of pancreatin cell culture medium to terminate digestion. And repeatedly and slightly blowing with a suction tube until cells are uniformly dispersed, transferring the cell suspension to a 15mL centrifuge tube, centrifuging (1000 rpm,5min at room temperature), discarding supernatant, adding a cell culture medium, uniformly blowing the cells, transferring the cells to a cell culture bottle according to corresponding passage proportion for continuous culture, and selecting the cells in good state for cell freezing.
(3) Cell cryopreservation
Frozen stock solution: 5% (v/v) DMSO+95% complete medium (DMEM+10% FBS). Collecting cells according to a passage way, after centrifuging the cells, re-suspending the cells by adopting a frozen stock solution, and adjusting the concentration of the cells to be 2 multiplied by 10 7 The samples were transferred to 2mL cryopreservation tubes. Putting the freezing tube into a freezing box containing isopropanol, and carrying out gradient cooling freezing: standing at 4deg.C for 0.5 hr, standing at-20deg.C for 2 hr, standing at-80deg.C overnight, and transferring the frozen tube into liquid nitrogen for long-term storage.
Example 2 scratch test
Wound healing assays, also commonly referred to as "scratch assays", are a widely established technique to study collective cell migration in vitro. The invention adopts scratch experiments to evaluate and compare the influence of peach gum polysaccharide on cell migration. The principle of the experiment is that when cells grow to be fused into a single-layer state, a blank area called a scratch is artificially manufactured on the fused single-layer cells, and the cells at the edge of the scratch gradually enter the blank area to heal the scratch. Cell types for wound healing assays aimed at restoring regenerative characteristics of the epidermis, immortalized cell lines and primary cells were often used as models in this regard, the most common cell type being the fibroblast L929 cell.
Recurrent inflammation also delays wound healing, whereas the body's inflammatory response is accompanied by the release of inflammatory mediators, nitric oxide being the primary inflammatory mediator, and thus reducing the production of nitric oxide, the pro-inflammatory mediator, is critical in inhibiting the inflammatory response. Macrophages are involved in the body's defense mechanisms, and they rapidly secrete a variety of inflammatory mediators, affecting the development of the inflammatory response, and are often used as models for this aspect as RAW264.7 cells.
The invention uses L929 cells (purchased from Qiao Xin boat biotechnology Co., ltd. In Shanghai) to evaluate and compare the effect of peach gum polysaccharide on cell migration by detecting the closing speed of cell gaps, and verify the effect of peach gum polysaccharide on wound healing. Peach gum polysaccharide with concentration of 0.5mg/mL, 1mg/mL and 2mg/mL is taken for wound healing test.
(1) Preparation of an orifice plate: in order to record the position of the subsequent photographing conveniently, the back surface of the pore plate needs to be marked. The 6-hole plate, the 12-hole plate and the 24-hole plate can be used as scratch experiment hole plates, and the 24-hole plate is adopted in the experiment. Marking is carried out at the bottom of the pore plate by means of a ruler and a carving knife, and the bottom of the pore plate is divided into two parts in a bisection mode so as to record the position during shooting.
(2) Cell plating: after carrying out passage operation on L929 cells which grow well and reach 70% -75% of fusion degree, the cells are subjected to passage operation according to the method of 4 multiplied by 10 5 cell/well ratio was spread in a 24-well plate with a cell fluid volume of 2mL at 37℃and 5% CO 2 Culturing overnight (about 12-16 h). Notably, are: since the calculation of the subsequent scratches is based on the taken cell photographs, the cell density of the plating needs to be maintained to a certain degree of uniformity, the skill and steps of plating uniformity: 1. rinsing the pore plate: the gun head is slightly added with the culture medium against the wall of the pore plate, the pore plate can be slightly shaken to enable the culture medium to completely cover the bottom of the pore, and the culture medium is sucked and removed; 2. after the cells are digested and collected, the cells are ensured to be evenly blown away before being added into a pore plate, 1mL of culture medium is firstly added and lightly blown under 5-7 by a gun head, and then the culture medium is added and diluted to the required cell density; 3. the pipette was gently added to the cell suspension perpendicular to the well plate to allow uniform cell diffusion from the middle to the periphery. After the addition, the well plate was gently lifted, gently tapped with the palm edge around the well plate (4-5 f), and carefully transferred to the incubator for overnight incubation.
(3) Scratch drug administration: the 200 mu L gun head with a flat tip is selected, a cell monolayer at the bottom of the hole is gently scratched, and a gap with a flat edge and consistent can be obtained by means of a ruler. Cell debris was washed with PBS and repeated 3 times. Fresh serum-free medium was added to the blank experiments, photographs were taken for 0h, the photographing positions were recorded, the culture was returned to the incubator for further culture, and photographs were taken for 6, 12, 24, 30, 36, 48 h. In several polysaccharide comparison experiments, after PBS washing cell debris, the medium was replaced with 1mg/mL of a different polysaccharide solution dissolved in serum-free medium, and photographs were taken at 0h and 36 h.
(4) Photo processing and calculation: the photographed scratch photo is processed and calculated using Imagej software.
As shown in FIG. 1, the experimental results show that the composition (A) of FIG. 1 has better effect of promoting gap closure at 0.5mg/mL and 1mg/mL, and the composition (A) does not show the effect of promoting gap closure when the concentration is increased to 2 mg/mL; as can be seen from fig. 1 (B), the cell viability was tested, and it was found that the proliferation of cells was not affected by different concentrations of peach gum polysaccharide; as can be seen from fig. 1 (C), peach gum polysaccharide with a concentration of 1mg/mL was selected for testing, and it was found that the gap closure rate was positively correlated with wound healing; as can be seen from FIG. 1 (D), the L929 cells in the normal culture state have a cell morphology as shown in FIG. 1 (D) (left), and the cells are mainly in the form of a shuttle, which is not significantly different from the cell morphology of the present invention in which peach gum polysaccharide of 1mg/mL is added.
Therefore, the peach gum polysaccharide provided by the invention can promote the migration of L929 cells to a scratch area under the condition that the proliferation and the morphology of the L929 cells are not influenced, and has better promotion effect when the concentration is 0.5mg/mL and 1mg/mL, and the gap closure rate reaches 56% and is 1.5 times of that of a blank group.
In conclusion, the experimental results show that the peach gum polysaccharide with different concentrations has better effect of promoting gap closure, and further experiments show that the peach gum polysaccharide has the effects of potential wound healing and skin injury repair under the condition that cell proliferation and cell morphology are not affected.
Example 3 experiment for verifying anti-inflammatory Activity of peach gum polysaccharide
The invention verifies the reaction of peach gum polysaccharide to inflammation by detecting the nitric oxide content of lipopolysaccharide-induced macrophages (RAW 264.7 cells, purchased from Qiao Xin boat biotechnology Co., ltd.) in Shanghai.
The anti-inflammatory activity of peach gum polysaccharide was studied using RAW264.7 cells. In logarithmic growthRAW264.7 cells with good growth status were digested with pancreatin and then subjected to 2.5X10 5 The cells/well were inoculated into 6-well plates and cultured for 24 hours. After the culture is finished, the culture medium is replaced by peach gum polysaccharide culture medium with the concentration of 0mg/mL, 0.5mg/mL and 1mg/mL respectively, and the culture medium is incubated for 24 hours. After the end of the culture, the cells were stimulated with 2. Mu.g/mL LPS for 24h. Taking 50 mu L of cell culture solution or NaNO with different concentrations 2 50 mu L of standard substance is added into a 96-well plate, 50 mu L of Griess reagent I and Griess reagent II are added in sequence, after mixing reaction, the absorbance at 540nm is detected, and according to NaNO 2 And calculating the NO content by using a standard curve.
The experimental results are shown in fig. 2, and RAW264.7 cells under normal culture are shown in fig. 2 (a), and macrophage morphology is round and fusiform; the effect of peach gum polysaccharide on nitric oxide content of lipopolysaccharide-induced RAW264.7 cells is shown in fig. 2 (B). The nitric oxide content of the cells in the blank group is less than 7 mu M, and after being stimulated by lipopolysaccharide, the nitric oxide content of macrophages treated by peach gum polysaccharide with the nitric oxide content of up to 48 mu M,0.5 and 1mg/mL is obviously reduced.
Therefore, the peach gum polysaccharide provided by the invention can inhibit nitric oxide secretion and has anti-inflammatory activity.
In conclusion, the peach gum polysaccharide has the effects of improving skin inflammation and repairing skin injury.
EXAMPLE 4 preparation of peach gum polysaccharide
The preparation method of the peach gum polysaccharide comprises the following steps:
(1) Soaking and swelling: adding pure water into the peach gum, uniformly stirring, soaking the peach gum until the peach gum is completely swelled after the peach gum is completely wetted, and cleaning impurities; the feed liquid ratio of the peach gum to the pure water is 1g to 25mL; swelling time is 4h;
(2) Heating and decocting: adding pure water into the peach gum cleaned in the step (1), and heating and decocting to obtain a feed liquid 1; the feed liquid ratio of the peach gum to the pure water is 1g to 50mL; the decoction temperature is 60 ℃ and the decoction time is 1.5h;
(3) Homogenizing under high pressure: heating the step (2) to obtain feed liquid 1, and homogenizing under high pressure to completely dissolve and disperse peach gum to obtain feed liquid 2; the homogenizing pressure is 60Bar, the homogenizing temperature is 121 ℃, and the homogenizing time is 0.5h;
(4) And (3) suction filtration: carrying out suction filtration on the feed liquid 2 obtained in the step (3) to remove insoluble impurities, thereby obtaining polysaccharide extract;
(5) Ultrafiltration and freeze-drying: and (3) carrying out ultrafiltration concentration on the polysaccharide extract obtained in the step (4), collecting peach gum polysaccharide with molecular weight more than 3kDa, and freeze-drying to obtain the peach gum polysaccharide.
EXAMPLE 5 preparation of peach gum polysaccharide
The preparation method of the peach gum polysaccharide comprises the following steps:
(1) Soaking and swelling: adding pure water into the peach gum, uniformly stirring, soaking the peach gum until the peach gum is completely swelled after the peach gum is completely wetted, and cleaning impurities; the feed liquid ratio of the peach gum to the pure water is 1g to 50mL; swelling time is 12h;
(2) Heating and decocting: adding pure water into the peach gum cleaned in the step (1), and heating and decocting to obtain a feed liquid 1; the feed liquid ratio of the peach gum to the pure water is 1g to 100mL; the decoction temperature is 80 ℃ and the decoction time is 2 hours;
(3) Homogenizing under high pressure: heating the step (2) to obtain feed liquid 1, and homogenizing under high pressure to completely dissolve and disperse peach gum to obtain feed liquid 2; the homogenizing pressure is 100Bar, the homogenizing temperature is 150 ℃, and the homogenizing time is 2.5h;
(4) And (3) suction filtration: carrying out suction filtration on the feed liquid 2 obtained in the step (3) to remove insoluble impurities, thereby obtaining polysaccharide extract;
(5) Ultrafiltration and freeze-drying: and (3) carrying out ultrafiltration concentration on the polysaccharide extract obtained in the step (4), collecting peach gum polysaccharide with molecular weight more than 3kDa, and freeze-drying to obtain the peach gum polysaccharide.
Example 6 Property experiment of peach gum polysaccharide
In the experimental process, the peach gum polysaccharide prepared in the example 4 is adopted for experiments, and the properties of the peach gum polysaccharide are measured; the peach gum is produced from Fujian.
1. Solubility experiment of peach gum polysaccharide: a proper amount of peach gum polysaccharide prepared in example 4 is taken and dissolved in 100g of water at 20 ℃ and normal pressure for solubility experiment, 30g of peach gum polysaccharide can be dissolved, the solubility of the prior art peach gum is only 5% -6%, and the solubility of the peach gum polysaccharide prepared by the method is improved to 30%, so that the prepared peach gum polysaccharide has good solubility.
2. Monosaccharide composition analysis: peach gum polysaccharide or monosaccharide standard (arabinose, rhamnose, fucose, xylose, mannose, galactose, galacturonic acid, glucose, glucuronic acid) with corresponding amount is weighed into brown vial (sample amount is 2 mg), 100 μl inositol (1 mg/mL) is added as internal standard, and vacuum freeze-dried at-80deg.C. After drying, 1mL of anhydrous methanol hydrochloride was added, the tube was sealed with nitrogen, and after 24 hours of reaction at 80℃the methanol was evaporated with nitrogen. After methanol was volatilized, 1mL of anhydrous methanol was added to wash the sample and blow-dried, and repeated three times. 200. Mu.L of TMS derivatizing agent (hexamethyldisilazane: chlorotrimethylsilane: pyridine in a volume ratio of =2:1:5, after mixing, preservation at-20 ℃ C.) was added, and after mixing, the mixture was allowed to stand for 30min, centrifuged at 6000rpm for 2min, and 1. Mu.L of the supernatant was taken for gas chromatography. Analysis conditions: chromatographic column, DB-5 capillary column (30 m. Times.0.25 mm; agilent technology, USA); heating program: heating from 140 ℃ to 170 ℃ at 1 ℃/min to 250 ℃ at 6 ℃/min, then heating to 260 ℃ at 30 ℃/min, and keeping for 3min; sample introduction temperature: 260 ℃; detector temperature: 260 ℃; carrier gas: nitrogen gas; flow rate: the split ratio was 20:1 at 0.9 mL/min.
Qualitative analysis was performed using inositol as an internal standard using relative retention time, i.e., the retention time of each standard monosaccharide relative to the internal standard inositol. And drawing a standard curve by taking the concentration (x) of the monosaccharide standard substance as an abscissa and the relative peak area (the peak area of the monosaccharide standard substance: the inner peak area (y) of inositol) as an ordinate to obtain a linear formula (table 1), and calculating the content of each monosaccharide in the peach gum polysaccharide, thereby calculating the mole percentage of the contained monosaccharide.
Table 1 9 Linear formulas for trimethylsilyl ether derivatives of standard monosaccharides
| Class of monosaccharides | Linear formula | Relative retention time |
| Arabinose (Arabic sugar) | y＝0.4x-0.04 | 0.33 |
| Rhamnose (rhamnose) | y＝0.67x-0.09 | 0.36 |
| Fucose | y＝0.35x-0.03 | 0.39 |
| Xylose | y＝0.49x-0.05 | 0.46 |
| Mannose | y＝0.67x-0.03 | 0.67 |
| Galactose | y＝0.33x-0.05 | 0.75 |
| Galacturonic acid | y＝0.22x-0.02 | 0.81 |
| Glucose | y＝0.47x | 0.83 |
| Glucuronic acid | y＝0.27x-0.02 | 0.87 |
FIG. 3 is a gas chromatogram of peach gum polysaccharide prepared in example 4 of the present invention. As shown in figure 3, the peach gum polysaccharide prepared by the invention mainly consists of arabinose, galactose and xylose, and also contains a small amount of glucuronic acid, mannose and rhamnose. Wherein, compared with the monosaccharide standard, the arabinose content is higher, the galactose and glucuronic acid content is reduced, and the arabinose, galactose, xylose, glucuronic acid, mannose and mouse Li Tangma mole percent are 57.4%, 25.9%, 10.9%, 2.2%,1.9% and 1.7%, respectively.
Therefore, the peach gum polysaccharide provided by the invention has rich arabinose content, better emulsifying capacity and stability, and further expands the application of the peach gum polysaccharide in cosmetics.
3. Rheological property analysis: the rheological properties were measured using a rotary rheometer (DHR-2 DHR-2, wolts China Co.). The rheometer detection conditions were: the diameter was 4cm, the gap was 1mm and the temperature was 25 ℃.
Shear stress test: the shear rate is in the range of 0.1 to 100s -1 Detecting the change of the shear stress of the sample along with the change of the shear rate, and carrying out regression fitting on data points by using a power law equation, wherein the equation is used for describing the rheological property of the pseudo-plastic fluid and is as follows:
τ＝Kγn
wherein: τ is the shear stress (Pa); gamma is the shear rate(s) -1 ) The method comprises the steps of carrying out a first treatment on the surface of the K is the consistency coefficient (Pa.sn); n is the flow index.
Viscoelasticity detection: before testing, the frequency is set to be 1Hz, and a strain sweep test is carried out, wherein the strain range is 0.1-100%, so as to determine the linear viscoelastic region between the shear stress and the shear strain. In the defined linear viscoelastic region, the strain value was defined as 0.3%. The strain value was set to 0.3%, and a frequency sweep test was performed in a frequency range of 0.1 to 100rad/s to detect changes in the storage modulus (G ') and loss modulus (G') of the sample.
Temperature scan test: the sample was tested for changes in storage modulus (G ') and loss modulus (G') at a frequency of 1Hz and a strain value of 0.3% at 15-80℃by covering the sample with a low concentration of silicone oil during the test to prevent evaporation of water.
Fig. 4 is a graph of rheological properties of peach gum polysaccharide in solutions of different concentrations, wherein fig. 4 (a) is a graph of shear stress versus shear rate, and fig. 4 (B) is a graph of viscosity versus shear rate. As shown in fig. 4, the peach gum polysaccharide at the concentration of 1% -4% shows typical shear thinning behavior, namely, the viscosity is reduced with the increase of the shear rate, because the intermolecular entanglement breaking rate is greater than the recombination rate, so that the intermolecular flow resistance is reduced, and the viscosity is reduced, so that the peach gum polysaccharide can be judged to be a pseudoplastic non-newtonian fluid. The shear thinning characteristic ensures that the product has better smearing performance when in use, and is beneficial to further application of peach gum polysaccharide in cosmetics.
The pseudoplastic property of the peach gum polysaccharide system can be subjected to regression fit by using a power law equation, so that a consistency index (K) and a flow index (n) are obtained. As shown in table 2, the consistency index (K) and the flow index (n) of the peach gum polysaccharide at different concentrations are given in table 2. The result shows that the power law equation fitting correlation coefficient R of peach gum polysaccharide 2 All are larger than 0.99, which shows that the model has better simulation precision on the static rheological property of the peach gum polysaccharide. The consistency index K value increases with the increase of the concentration of the peach gum polysaccharide, which shows that the viscosity of the peach gum polysaccharide becomes larger with the increase of the concentration; in contrast, the fluid index n of the peach gum polysaccharide is reduced along with the increase of the concentration of the peach gum polysaccharide, and n is smaller than 1, so that the peach gum polysaccharide is further proved to be pseudoplastic fluid. At the same time, n can intuitively reflect the shearing resistance of the sample, and the smaller n is, the stronger the shearing resistance is, and the adhesive force is correspondingly improved, so that for products such as foundations, concealers and the like which need to be adhered on the surface layer of the skin for a long time, the products with smaller n value can lead the products to be difficult to deform, andto better and stable adhesion to the skin surface, facilitating application in such products.
TABLE 2 fitting parameters for the shear resistance of peach gum polysaccharides at different concentrations
| Peach gum concentration (%) | K(Pa·s n ) | n | R 2 |
| 1 | 0.077±0.002 | 0.719±0.006 | 0.99 |
| 2 | 0.144±0.006 | 0.713±0.009 | 0.99 |
| 3 | 0.485±0.019 | 0.622±0.01 | 0.99 |
| 4 | 1.037±0.040 | 0.562±0.009 | 0.99 |
Therefore, the peach gum polysaccharide provided by the invention has the phenomenon of shear thinning, can be more easily spread and smeared when being added into cosmetics, and can be easily made up when being applied to cosmetics, especially foundation make-up liquid, and can be more adhered to skin.
EXAMPLE 7 preparation of peach gum polysaccharide Water-yielding cream
The preparation of the water outlet cream containing peach gum polysaccharide comprises the following steps:
(1) Preparing a solution: 1.6g of PEG-10 polydimethylsiloxane, 10g of cyclopentadimethylsiloxane, 1g of caprylic triglyceride, 1g of simethicone and 0.06g of essence are weighed and uniformly stirred to prepare a phase A, wherein the phase A is an oil phase for later use; weighing 16g of glycerin, 10g of propylene glycol, 0.6g of peach gum polysaccharide prepared in example 4 and 156.68g of water according to the formula amount, and uniformly stirring and mixing to prepare a phase B for later use; weighing 2g of sodium chloride, 0.8g of p-hydroxyacetophenone, 0.06g of gardenia blue pigment and 0.2g of ethylhexyl glycerol according to the formula, and uniformly stirring to prepare a C phase, wherein the C phase is a water phase for later use;
(2) Adding phase B into phase C, heating, stirring for dissolving, slowly pouring the dissolved mixed raw material into phase A, and stirring uniformly;
(3) Stopping stirring to obtain the water cream containing peach gum polysaccharide.
Example 8 preparation of peach gum polysaccharide Water-yielding cream
The preparation of the water outlet cream containing peach gum polysaccharide comprises the following steps:
(1) Preparing a solution: 1.8g of PEG-10 polydimethylsiloxane, 12g of cyclopentadimethylsiloxane, 2.5g of caprylic triglyceride of capric acid, 2.5g of simethicone and 0.06g of essence are weighed and uniformly stirred to prepare a phase A, wherein the phase A is an oil phase for later use; weighing 14g of glycerol, 4g of propylene glycol, 0.3g of peach gum polysaccharide prepared in example 4 and 156.51g of water according to the formula amount, and stirring and uniformly mixing to prepare a phase B for later use; weighing 4g of sodium chloride, 1.1g of p-hydroxyacetophenone, 0.15g of gardenia blue pigment and 0.3g of ethylhexyl glycerol according to the formula amount, and stirring and uniformly mixing to prepare a C phase, wherein the C phase is a water phase for standby;
(2) Adding phase B into phase C, heating, stirring for dissolving, slowly pouring the dissolved mixed raw material into phase A, and stirring uniformly;
(3) Stopping stirring to obtain the water cream containing peach gum polysaccharide.
Example 9 preparation of peach gum polysaccharide free Water-yielding cream
Compared with example 7, the water cream of this example was not added with peach gum polysaccharide, and the same as example 7 was followed.
Example 10 appearance and Cold-resistant Heat stability test of peach gum containing polysaccharide Water bloom of the invention
The water outlet cream prepared in the embodiment 7 of the invention is taken for experiments, and the water outlet cream prepared in the embodiment 9 is taken as a blank control group, and the experimental method is as follows:
heat stability test: taking the completely packaged water outlet cream, placing the water outlet cream in a constant-temperature oven at 40 ℃, taking out the water outlet cream after 24 hours, cooling the water outlet cream to room temperature, and performing visual observation; cold resistance stability: taking out the completely packaged water outlet frost, placing the water outlet frost in a refrigerator at the temperature of minus 10 ℃, taking out the water outlet frost after 24 hours, and carrying out visual observation after the water outlet frost is restored to the room temperature.
The experimental result is shown in figure 5, the appearance of the water cream containing peach gum polysaccharide provided by the invention is light blue, the texture is thick, and the paste is fine; after the peach gum polysaccharide-containing water cream is placed for 24 hours at the temperature of minus 10 ℃ and 40 ℃, the phenomena of oil slick and layering do not occur, and the color does not change obviously, so that the peach gum polysaccharide-containing water cream is stable.
In conclusion, the peach gum-containing polysaccharide provided by the invention has better heat-resistant and cold-resistant stability.
EXAMPLE 11 static rheological property experiment of peach gum-containing polysaccharide water bloom
The shear rate of a fluid is mainly related to the temperature, viscosity and type of fluid, and the ratio of shear stress to shear rate is called absolute viscosity, which is a measure of the flow resistance of the fluid.
The rheological properties of cosmetic products are related to their long-term stability and skin usage feel, the maximum viscosity of the product affects the primary skin feel (i.e. feel from the product container to the skin) at the time of use, while the skin feel (i.e. secondary skin feel) of the product from application to the absorption stage at the skin top layer is related to the shear applied during application, which needs to be determined by measuring the viscosity at high shear rates.
The viscosity of the peach gum-containing polysaccharide water outlet cream of the embodiment 7 is measured by adopting a rotary viscometer, and the water outlet cream prepared in the embodiment 9 is used as a blank control. The viscosity was measured using a rotational viscometer. Because the peach gum viscosity is increased along with the increase of the concentration, rotors with different numbers are selected according to the difference of the peach gum viscosity, and the basis of the rotors is selected to ensure that the opening angle range is between 15% and 85%; the rotation speed was 12rpm/min, the detection time was 2min, and the viscosity value was obtained.
The experimental results of static rheological properties of the peach gum polysaccharide-containing water bloom are shown in fig. 6, and as can be seen from fig. 6, the addition of the peach gum polysaccharide improves the relevant rheological properties of the water bloom; in particular, the viscosity of the product of FIG. 6 (A) decreases with increasing shear rate and gradually stabilizes. The product can be judged to be pseudo plastic fluid, and has the phenomenon of shear thinning, and the phenomenon ensures that the product forms a layer of film on the surface of skin, so that the smearing and the absorption of the product are accelerated. At low shear rates, the product has a higher viscosity, up to 450 Pa.s, which also contributes to the long-term stability of the product. FIG. 6 (B) shows that the peach gum product has a shear rate of 1-100 s -1 Change in lower shear stress. From the results, the shearing stress of the product is 1 to 100 seconds -1 The increasing and decreasing trend is shown in the shearing rate range, the adding of peach gum polysaccharide slows down the rising speed of the shearing stress, but both of the peach gum polysaccharide and the peach gum polysaccharide have a sharp turning when the shearing stress reaches 210Pa, which indicates that the internal structure of the product is changed at the moment.
The peach gum polysaccharide water-out cream provided by the invention has the phenomenon of shear thinning, and the lower product viscosity has better skin feeling under the high shear rate, so that the product is more beneficial to the long-term stability, a layer of film can be formed on the skin surface, the smearing and the absorption of the product are accelerated, and the water-out cream product has better smearing effect and skin feeling in use.
EXAMPLE 12 dynamic rheology test of peach gum-containing polysaccharide water bloom
The lower gel structure of the product can reduce the obstruction of the active ingredient to the skin in terms of the use level of the cosmetic product, thereby increasing the contact between the active ingredient and the skin and better playing the active protection role. The rheological property of the peach gum polysaccharide cream is measured and evaluated by adding 0.3% of peach gum polysaccharide (prepared in example 7), and the water outlet cream without the peach gum polysaccharide prepared in example 9 is used as a blank control. Gel properties of the water cream were reflected by oscillatory shear for evaluating the elastic modulus (G') and viscous modulus (G ") of the product.
And detecting rheological characteristics by adopting a rotary rheometer. The rheometer detection conditions were: the diameter was 4cm, the gap was 1mm and the temperature was 25 ℃.
Shear stress test: the shear rate is in the range of 0.1 to 100s -1 Detecting the change of the shear stress of the sample along with the change of the shear rate, and carrying out regression fitting on data points by using a power law equation, wherein the equation is used for describing the rheological property of the pseudo-plastic fluid and is as follows:
τ＝Kγn
wherein: tau is the shear stress (Pa)
Gamma is shear rate (s-1)
K is the consistency coefficient (Pa, sn)
n is the flow index
Viscoelasticity detection: before testing, the frequency is set to be 1Hz, and a strain sweep test is carried out, wherein the strain range is 0.1-100%, so as to determine the linear viscoelastic region between the shear stress and the shear strain. In the defined linear viscoelastic region, the strain value was defined as 0.3%. The strain value was set to 0.3%, and a frequency sweep test was performed in a frequency range of 0.1 to 100rad/s to detect changes in the storage modulus (G ') and loss modulus (G') of the sample.
Temperature scan test: the sample was tested for changes in storage modulus (G ') and loss modulus (G') at a frequency of 1Hz and a strain value of 0.3% at 15-80℃by covering the sample with a low concentration of silicone oil during the test to prevent evaporation of water.
As shown in FIG. 7, the results of the dynamic rheological property experiments of the peach gum polysaccharide-containing water cream are shown in FIG. 7 (A), the elasticity of the two water creams is better than the viscosity, and the deformation modulus of the blank control group (the water cream product prepared in example 9) and the 0.3% peach gum polysaccharide water cream prepared in example 7 are observed to have weak frequency dependence according to the results, so that the gel structure exists in the two products. The elastic modulus is a main contribution parameter of cohesive energy of the emulsion system, and can be used for reflecting the strength of a system network structure, and experimental results show that the elastic modulus of the water-out cream added with peach gum polysaccharide in the embodiment 7 of the invention is reduced, which indicates that the structural strength of a gel network is reduced compared with that of a control group.
Tan δ=tan (G "/G') can be used to reflect the change in solid-like elastic properties in emulsion systems, the smaller Tan δ, the more pronounced the elastic properties. As can be seen from FIG. 7 (B), within 0.1-2 rad/s, delta of the peach gum-containing polysaccharide water bloom provided by the invention is sharply reduced along with the increase of frequency, while within 2-100 rad/s, the delta is gradually increased along with the increase of frequency, and the reason for the phenomenon is probably due to the instability of a product emulsifying system. However, tan delta was less than 0.2, and the blank group water outlet cream (example 9) showed a weak gel behavior with substantially the same variation trend of Tan delta as the peach gum-containing polysaccharide water outlet cream (example 7), and delta was less than 45 °.
Therefore, the dynamic rheology test result shows that the elasticity of the water cream containing peach gum polysaccharide is better than the viscosity, and the addition of the peach gum polysaccharide enables the water cream to have weak gel behavior, increases the contact between the active ingredients of the water cream and the skin, and plays a better role in active protection.
In conclusion, the addition of the peach gum polysaccharide has certain improvement effects on the viscosity, the flow property and the gel structure of the water cream, so that the peach gum polysaccharide can form a film on the surface layer of the skin more quickly, and the peach gum polysaccharide is more beneficial to smearing and absorption of the product.
Example 13 skin sensory evaluation and efficacy evaluation experiment of peach gum polysaccharide-containing Water bloom of the present invention
(1) Sensory evaluation of skin
The skin feel of the peach gum-containing polysaccharide water outlet cream provided by the invention is evaluated according to the general rule for sensory evaluation of cosmetics issued by the cosmetic society of Guangdong province.
15 volunteers are recruited, the basic requirements of the evaluation staff of the general rule of cosmetic sensory evaluation are met, no skin disease exists, and the hands are not easy to be allergic or eczematous. After washing hands and faces, wiping skin with paper towel, waiting for 5-10min under test environment (constant temperature and humidity environment: temperature 22+ -2deg.C, environment humidity 50+ -5% RH), and drying skin to normal state. Then 100 mu L of sample to be measured is taken and applied on the back of the hand, the finger is applied on the whole face, the sample is evenly applied for 10s clockwise from inside to outside, and the application part is required to be kept completely still. The observation index includes transparency, moisture retention, gloss, smoothness, freshness and compactness, and is expressed by 0 to 1 minute. Before testing, the descriptive vocabulary and scoring scale of the relevant indexes are explained and described in detail for the evaluator, so that the evaluator is ensured to grasp the correct evaluation steps. The relevant evaluation index and its description are shown in table 3.
Table 3 peach gum polysaccharide water-out cream skin feel evaluation table
The scoring results of the skin feel evaluation indexes are shown in fig. 8, wherein fig. 8 (a) is a scoring average value of the skin feel evaluation indexes, and in order to more intuitively evaluate the effect of peach gum polysaccharide on improving the skin feel of the face cream, the scoring results are converted into a radar chart shown in fig. 8 (B). As can be seen from FIG. 8, the peach gum polysaccharide water-out cream has similar transparency, moisture retention and gloss as the control product, and the smooth feel score is reduced compared with the control product, but the peach gum polysaccharide water-out cream provided by the invention has better refreshing degree and tightening feel.
(2) Efficacy evaluation
Many long-term skin with inflammation, after the inflammation is reduced, the skin is finer than before, pores are also coarser, one of the reasons is that various reactions caused by the inflammation are caused, if the inflammation is not inhibited, the skin is inflamed to cause local tissue necrosis, liquefaction, defect formation and acne pit formation of the skin. Skin care products only provide insight from the point of view of daily care and prevention. When the oil secretion is excessive, pores are enlarged, pores are further blocked, and various skin inflammations such as seborrheic dermatitis, red acne marks, skin redness and itching are formed, so that the sebum overflow of the skin is reduced, the pores can be effectively reduced, and the possibility of skin inflammation is reduced.
Among them, skin myobasal injury/inflammation is classified into three grades of mild, moderate and severe, which are specifically expressed as:
light: the temperature change causes the whole area to instantly reddish with discomfort, such as: the air conditioning room enters outdoors in summer or enters the air conditioning room outdoors in winter, the skin state is unstable, the skin barrier protective force is reduced, and the like;
and (3) moderately: pi Fufan with obvious discomfort, such as heat, dryness, itching; coarse pores, rough and dull skin;
severe: pi Fufan, and the reddish areas show inflammatory color deposition and skin inflammatory aging.
The invention adopts the peach gum polysaccharide-containing water outlet cream prepared in the embodiment 6 to carry out experiments, and the skin reaction conditions before and after the water outlet cream is smeared are tested, and the specific steps are as follows:
30 men and women with ages of 25-40 are selected, and skin reaction conditions before and after the cream is smeared are tested on the skin of the same area of a subject by using a skin tester, and the test is designed to be performed between 0 and 7 hours for six times and three continuous days. Test items: skin grease, skin pores, skin sensitivity.
TABLE 4 evaluation of skin reaction conditions before and after applying Water bloom
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Experiments are carried out by adopting the peach gum polysaccharide-containing water outlet cream prepared in the embodiment 7 of the invention, the peach gum polysaccharide-free water outlet cream prepared in the comparative example 1 is used as a blank control, the experimental results are shown in fig. 9, as can be seen from fig. 9 (A), the peach gum polysaccharide-containing water outlet cream and the blank control water outlet cream can both play a role in reducing skin pores after 10 minutes of smearing, but the blank control water outlet cream reduces skin pores to 9% after 7 hours, the reduction is 17%, and the peach gum polysaccharide-containing water outlet cream can reduce skin pores to 3%, and the reduction is 20%. As shown in fig. 9 (B), the skin sensitivity of both the water-out cream of the blank group and the water-out cream containing peach gum polysaccharide of the present invention can be restored, and compared with the water-out cream of the blank group, the water-out cream containing peach gum polysaccharide of the present invention has a better effect and can be reduced to 46%. As shown in fig. 9 (C), compared with the control group, the control oil of the water outlet cream for 10min is 39%, the control oil of the water outlet cream containing peach gum polysaccharide of the invention is 60%, the skin oil content can be reduced by 53% by 7h, and the control group water outlet cream is reduced by 38%.
Therefore, the experimental result shows that in the aspect of skin feel, the peach gum polysaccharide-containing water outlet cream and the blank control group water outlet cream have similar transparency, moisture retention, gloss and smoothness, but the peach gum polysaccharide-containing water outlet cream has better refreshing degree and compactness than the blank control group water outlet cream; in the aspect of efficacy, the peach gum polysaccharide water-out cream provided by the invention has a certain oil control effect, can shrink and tighten pores, and can improve sensitive skin to a certain extent, so that the peach gum polysaccharide can reduce the possibility of inflammation occurrence and promote the repair of skin injury.
The above embodiments are merely illustrative of the principles of the present invention and its effectiveness, and are not intended to limit the invention. Modifications and variations may be made to the above-described embodiments by those skilled in the art without departing from the spirit and scope of the invention. Accordingly, it is intended that all equivalent modifications and variations of the invention be covered by the claims, which are within the ordinary skill of the art, be within the spirit and scope of the present disclosure.
Claims (8)
1. Use of peach gum polysaccharide in products for promoting tissue repair or improving aesthetic skin.
2. The use according to claim 1, wherein the product is a pharmaceutical or cosmetic product.
3. The use according to claim 1, wherein the pharmaceutical or cosmetic dosage form comprises a gel cream, ointment, external liniment, patch, cream, emulsion, gel or toner.
4. The use according to claim 1, wherein the preparation method of peach gum polysaccharide comprises the following steps:
(1) Soaking and swelling: adding pure water into the peach gum, uniformly stirring, soaking the peach gum until the peach gum is completely swelled after the peach gum is completely wetted, and cleaning impurities; the feed liquid ratio of the peach gum to the pure water is 1 g:25-50 mL; the swelling time is 4-12 h;
(2) Heating and decocting: adding pure water into the peach gum cleaned in the step (1), and heating and decocting to obtain a feed liquid 1; the feed liquid ratio of the peach gum to the pure water is 1 g:50-100 mL; the decoction temperature is 60-80 ℃ and the decoction time is 1.5-2 h;
(3) Homogenizing under high pressure: heating the step (2) to obtain feed liquid 1, and homogenizing under high pressure to completely dissolve and disperse peach gum to obtain feed liquid 2; the homogenizing pressure is 60-100 Bar, the homogenizing temperature is 121-150 ℃, and the homogenizing time is 0.5-2.5 h;
(4) And (3) suction filtration: carrying out suction filtration on the feed liquid 2 obtained in the step (3) to remove insoluble impurities, thereby obtaining polysaccharide extract;
(5) Ultrafiltration and freeze-drying: and (3) carrying out ultrafiltration concentration on the polysaccharide extract obtained in the step (4), collecting peach gum polysaccharide with molecular weight more than 3kDa, and freeze-drying to obtain the peach gum polysaccharide.
5. The use according to claim 1, wherein the peach gum polysaccharide is added to the product in an amount of 0.5-5 w%.
6. A cosmetic containing peach gum polysaccharide is characterized by comprising, by weight, 0.05-0.2% of skin feel regulator, 0.2-1.2% of emulsifier, 4-8% of thickener, 8-20% of humectant, 0.5-2.5% of softener, 0.05-0.6% of preservative, 0.01-0.1% of colorant, 0.01-0.1% of essence, 0.5-2.0% of peach gum polysaccharide and the balance of water.
7. The peach gum polysaccharide-containing cosmetic according to claim 6, wherein the skin feel modifier is ethylhexyl glycerol;
the emulsifier is one or more of stearyl alcohol polyether-2, oleyl alcohol polyether-10, methoxy PEG/PPG-25/4 polydimethylsiloxane and PEG-10 polydimethylsiloxane;
the thickener comprises one or more of alginic acid and its salt, pectin, sodium hyaluronate, cetyl alcohol polyoxyethylene (3) ether, sodium chloride, potassium chloride, p-hydroxyacetophenone, and cyclopenta-dimethicone;
The humectant comprises one or more of sorbitol, glycerol, propylene glycol, butanediol, xylitol, sodium lactate, hyaluronic acid, pullulan, ceramide and triglyceride;
the softening agent comprises one or more of C10-18 fatty acid triglyceride, caprylic acid triglyceride, dimethyl silicone oil, isopropyl lanolate, acetylated lanonol, isopropyl myristate, cetyl alcohol and stearyl alcohol;
the preservative comprises one or more of benzethonium chloride and p-hydroxyacetophenone;
the colorant is gardenia blue pigment.
8. The method for preparing a cosmetic containing peach gum polysaccharide according to claim 6, comprising the steps of:
(1) Preparing a solution: weighing emulsifier, thickener, softener and essence according to the formula amount, and uniformly stirring to prepare phase A, wherein the phase A is an oil phase for later use; weighing humectant, peach gum polysaccharide and water according to the formula amount, stirring and mixing uniformly to prepare a phase B for later use; weighing a thickening agent, a preservative, a colorant and a skin feel regulator according to the formula amount, and uniformly stirring and mixing to prepare a C phase, wherein the C phase is a water phase for later use;
(2) Adding phase B into phase C, heating, stirring for dissolving, slowly pouring the dissolved mixed raw material into phase A, and stirring uniformly;
(3) Stopping stirring to obtain cosmetic containing peach gum polysaccharide.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111454374A (en) * | 2020-04-29 | 2020-07-28 | 上海珈凯生物科技有限公司 | Peach gum extract with skin tightening effect, and preparation method and application thereof |
| CN115368484A (en) * | 2022-09-28 | 2022-11-22 | 水羊化妆品制造有限公司 | Preparation method of peach gum polysaccharide, application of peach gum polysaccharide and cosmetics |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111454374A (en) * | 2020-04-29 | 2020-07-28 | 上海珈凯生物科技有限公司 | Peach gum extract with skin tightening effect, and preparation method and application thereof |
| CN115368484A (en) * | 2022-09-28 | 2022-11-22 | 水羊化妆品制造有限公司 | Preparation method of peach gum polysaccharide, application of peach gum polysaccharide and cosmetics |
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