CN116548472A - Composite biocontrol strain seed treating agent, preparation method and application - Google Patents
Composite biocontrol strain seed treating agent, preparation method and application Download PDFInfo
- Publication number
- CN116548472A CN116548472A CN202310503745.9A CN202310503745A CN116548472A CN 116548472 A CN116548472 A CN 116548472A CN 202310503745 A CN202310503745 A CN 202310503745A CN 116548472 A CN116548472 A CN 116548472A
- Authority
- CN
- China
- Prior art keywords
- seed
- composite
- treating agent
- seeds
- agent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 230000000443 biocontrol Effects 0.000 title claims abstract description 73
- 239000002131 composite material Substances 0.000 title claims abstract description 70
- 238000002360 preparation method Methods 0.000 title description 22
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 100
- 241000196324 Embryophyta Species 0.000 claims abstract description 41
- 238000011282 treatment Methods 0.000 claims abstract description 39
- 201000010099 disease Diseases 0.000 claims abstract description 31
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims abstract description 31
- 238000000855 fermentation Methods 0.000 claims abstract description 30
- 230000004151 fermentation Effects 0.000 claims abstract description 30
- 238000002156 mixing Methods 0.000 claims abstract description 29
- 241000607479 Yersinia pestis Species 0.000 claims abstract description 23
- 239000000203 mixture Substances 0.000 claims abstract description 17
- 241000238631 Hexapoda Species 0.000 claims abstract description 16
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 15
- 241000193755 Bacillus cereus Species 0.000 claims abstract description 14
- 241000193388 Bacillus thuringiensis Species 0.000 claims abstract description 13
- 239000000463 material Substances 0.000 claims abstract description 13
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 11
- 150000001875 compounds Chemical class 0.000 claims abstract description 11
- 229940097012 bacillus thuringiensis Drugs 0.000 claims abstract description 9
- 239000007787 solid Substances 0.000 claims abstract description 9
- 230000001580 bacterial effect Effects 0.000 claims abstract description 7
- 239000000843 powder Substances 0.000 claims abstract description 4
- 239000007788 liquid Substances 0.000 claims abstract description 3
- 235000013311 vegetables Nutrition 0.000 claims abstract description 3
- 241000894006 Bacteria Species 0.000 claims description 38
- 239000011248 coating agent Substances 0.000 claims description 27
- 238000004321 preservation Methods 0.000 claims description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 15
- 238000000227 grinding Methods 0.000 claims description 12
- 239000002270 dispersing agent Substances 0.000 claims description 11
- 238000001035 drying Methods 0.000 claims description 11
- 239000000945 filler Substances 0.000 claims description 11
- 238000000034 method Methods 0.000 claims description 11
- 239000003381 stabilizer Substances 0.000 claims description 11
- 238000009736 wetting Methods 0.000 claims description 11
- 238000000576 coating method Methods 0.000 claims description 10
- 238000007873 sieving Methods 0.000 claims description 7
- 238000001694 spray drying Methods 0.000 claims description 7
- -1 black T Chemical compound 0.000 claims description 6
- 229910019142 PO4 Inorganic materials 0.000 claims description 5
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 5
- 239000010452 phosphate Substances 0.000 claims description 5
- 229920002401 polyacrylamide Polymers 0.000 claims description 5
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims description 4
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 4
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 4
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 4
- 229920002472 Starch Polymers 0.000 claims description 4
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 4
- 239000004202 carbamide Substances 0.000 claims description 4
- 238000007865 diluting Methods 0.000 claims description 4
- 239000003415 peat Substances 0.000 claims description 4
- 239000011591 potassium Substances 0.000 claims description 4
- 229910052700 potassium Inorganic materials 0.000 claims description 4
- 239000008107 starch Substances 0.000 claims description 4
- 235000019698 starch Nutrition 0.000 claims description 4
- BDHFUVZGWQCTTF-UHFFFAOYSA-M sulfonate Chemical compound [O-]S(=O)=O BDHFUVZGWQCTTF-UHFFFAOYSA-M 0.000 claims description 4
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 claims description 3
- 229920001661 Chitosan Polymers 0.000 claims description 3
- 229920002774 Maltodextrin Polymers 0.000 claims description 3
- 239000005913 Maltodextrin Substances 0.000 claims description 3
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 claims description 3
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 claims description 3
- PXEDJBXQKAGXNJ-QTNFYWBSSA-L disodium L-glutamate Chemical compound [Na+].[Na+].[O-]C(=O)[C@@H](N)CCC([O-])=O PXEDJBXQKAGXNJ-QTNFYWBSSA-L 0.000 claims description 3
- 229940035034 maltodextrin Drugs 0.000 claims description 3
- 235000013336 milk Nutrition 0.000 claims description 3
- 239000008267 milk Substances 0.000 claims description 3
- 210000004080 milk Anatomy 0.000 claims description 3
- 235000013923 monosodium glutamate Nutrition 0.000 claims description 3
- 229940073490 sodium glutamate Drugs 0.000 claims description 3
- ICLYJLBTOGPLMC-KVVVOXFISA-N (z)-octadec-9-enoate;tris(2-hydroxyethyl)azanium Chemical compound OCCN(CCO)CCO.CCCCCCCC\C=C/CCCCCCCC(O)=O ICLYJLBTOGPLMC-KVVVOXFISA-N 0.000 claims description 2
- VPGSXIKVUASQIY-UHFFFAOYSA-N 1,2-dibutylnaphthalene Chemical compound C1=CC=CC2=C(CCCC)C(CCCC)=CC=C21 VPGSXIKVUASQIY-UHFFFAOYSA-N 0.000 claims description 2
- LDMOEFOXLIZJOW-UHFFFAOYSA-N 1-dodecanesulfonic acid Chemical compound CCCCCCCCCCCCS(O)(=O)=O LDMOEFOXLIZJOW-UHFFFAOYSA-N 0.000 claims description 2
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims description 2
- KRKNYBCHXYNGOX-UHFFFAOYSA-L 2-(carboxymethyl)-2-hydroxysuccinate Chemical compound [O-]C(=O)CC(O)(C(=O)O)CC([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-L 0.000 claims description 2
- FHVDTGUDJYJELY-UHFFFAOYSA-N 6-{[2-carboxy-4,5-dihydroxy-6-(phosphanyloxy)oxan-3-yl]oxy}-4,5-dihydroxy-3-phosphanyloxane-2-carboxylic acid Chemical compound O1C(C(O)=O)C(P)C(O)C(O)C1OC1C(C(O)=O)OC(OP)C(O)C1O FHVDTGUDJYJELY-UHFFFAOYSA-N 0.000 claims description 2
- 229920001817 Agar Polymers 0.000 claims description 2
- 239000005995 Aluminium silicate Substances 0.000 claims description 2
- 241000416162 Astragalus gummifer Species 0.000 claims description 2
- 239000004375 Dextrin Substances 0.000 claims description 2
- 229920001353 Dextrin Polymers 0.000 claims description 2
- FEWJPZIEWOKRBE-JCYAYHJZSA-N Dextrotartaric acid Chemical compound OC(=O)[C@H](O)[C@@H](O)C(O)=O FEWJPZIEWOKRBE-JCYAYHJZSA-N 0.000 claims description 2
- 235000010643 Leucaena leucocephala Nutrition 0.000 claims description 2
- 240000007472 Leucaena leucocephala Species 0.000 claims description 2
- 229920001732 Lignosulfonate Polymers 0.000 claims description 2
- SJEYSFABYSGQBG-UHFFFAOYSA-M Patent blue Chemical compound [Na+].C1=CC(N(CC)CC)=CC=C1C(C=1C(=CC(=CC=1)S([O-])(=O)=O)S([O-])(=O)=O)=C1C=CC(=[N+](CC)CC)C=C1 SJEYSFABYSGQBG-UHFFFAOYSA-M 0.000 claims description 2
- 241000220317 Rosa Species 0.000 claims description 2
- NWGKJDSIEKMTRX-AAZCQSIUSA-N Sorbitan monooleate Chemical compound CCCCCCCC\C=C/CCCCCCCC(=O)OC[C@@H](O)[C@H]1OC[C@H](O)[C@H]1O NWGKJDSIEKMTRX-AAZCQSIUSA-N 0.000 claims description 2
- MKRNVBXERAPZOP-UHFFFAOYSA-N Starch acetate Chemical compound O1C(CO)C(OC)C(O)C(O)C1OCC1C(OC2C(C(O)C(OC)C(CO)O2)OC(C)=O)C(O)C(O)C(OC2C(OC(C)C(O)C2O)CO)O1 MKRNVBXERAPZOP-UHFFFAOYSA-N 0.000 claims description 2
- 229920001615 Tragacanth Polymers 0.000 claims description 2
- DPXJVFZANSGRMM-UHFFFAOYSA-N acetic acid;2,3,4,5,6-pentahydroxyhexanal;sodium Chemical compound [Na].CC(O)=O.OCC(O)C(O)C(O)C(O)C=O DPXJVFZANSGRMM-UHFFFAOYSA-N 0.000 claims description 2
- 239000000980 acid dye Substances 0.000 claims description 2
- 239000008272 agar Substances 0.000 claims description 2
- 235000010419 agar Nutrition 0.000 claims description 2
- 229940072056 alginate Drugs 0.000 claims description 2
- 235000010443 alginic acid Nutrition 0.000 claims description 2
- 229920000615 alginic acid Polymers 0.000 claims description 2
- 125000000217 alkyl group Chemical group 0.000 claims description 2
- 235000012211 aluminium silicate Nutrition 0.000 claims description 2
- CEGOLXSVJUTHNZ-UHFFFAOYSA-K aluminium tristearate Chemical compound [Al+3].CCCCCCCCCCCCCCCCCC([O-])=O.CCCCCCCCCCCCCCCCCC([O-])=O.CCCCCCCCCCCCCCCCCC([O-])=O CEGOLXSVJUTHNZ-UHFFFAOYSA-K 0.000 claims description 2
- SNAAJJQQZSMGQD-UHFFFAOYSA-N aluminum magnesium Chemical compound [Mg].[Al] SNAAJJQQZSMGQD-UHFFFAOYSA-N 0.000 claims description 2
- 229940063655 aluminum stearate Drugs 0.000 claims description 2
- 229960000892 attapulgite Drugs 0.000 claims description 2
- 239000000440 bentonite Substances 0.000 claims description 2
- 229910000278 bentonite Inorganic materials 0.000 claims description 2
- SVPXDRXYRYOSEX-UHFFFAOYSA-N bentoquatam Chemical compound O.O=[Si]=O.O=[Al]O[Al]=O SVPXDRXYRYOSEX-UHFFFAOYSA-N 0.000 claims description 2
- YYRMJZQKEFZXMX-UHFFFAOYSA-L calcium bis(dihydrogenphosphate) Chemical compound [Ca+2].OP(O)([O-])=O.OP(O)([O-])=O YYRMJZQKEFZXMX-UHFFFAOYSA-L 0.000 claims description 2
- 229910000019 calcium carbonate Inorganic materials 0.000 claims description 2
- 235000010216 calcium carbonate Nutrition 0.000 claims description 2
- 229910000389 calcium phosphate Inorganic materials 0.000 claims description 2
- 239000000378 calcium silicate Substances 0.000 claims description 2
- 229910052918 calcium silicate Inorganic materials 0.000 claims description 2
- OYACROKNLOSFPA-UHFFFAOYSA-N calcium;dioxido(oxo)silane Chemical compound [Ca+2].[O-][Si]([O-])=O OYACROKNLOSFPA-UHFFFAOYSA-N 0.000 claims description 2
- 239000006229 carbon black Substances 0.000 claims description 2
- 235000019241 carbon black Nutrition 0.000 claims description 2
- 239000001768 carboxy methyl cellulose Substances 0.000 claims description 2
- 229940045110 chitosan Drugs 0.000 claims description 2
- 235000019425 dextrin Nutrition 0.000 claims description 2
- 150000002191 fatty alcohols Chemical class 0.000 claims description 2
- 239000011790 ferrous sulphate Substances 0.000 claims description 2
- 235000003891 ferrous sulphate Nutrition 0.000 claims description 2
- 235000013905 glycine and its sodium salt Nutrition 0.000 claims description 2
- 239000004247 glycine and its sodium salt Substances 0.000 claims description 2
- 239000001341 hydroxy propyl starch Substances 0.000 claims description 2
- 239000001866 hydroxypropyl methyl cellulose Substances 0.000 claims description 2
- 235000010979 hydroxypropyl methyl cellulose Nutrition 0.000 claims description 2
- 229920003088 hydroxypropyl methyl cellulose Polymers 0.000 claims description 2
- UFVKGYZPFZQRLF-UHFFFAOYSA-N hydroxypropyl methyl cellulose Chemical compound OC1C(O)C(OC)OC(CO)C1OC1C(O)C(O)C(OC2C(C(O)C(OC3C(C(O)C(O)C(CO)O3)O)C(CO)O2)O)C(CO)O1 UFVKGYZPFZQRLF-UHFFFAOYSA-N 0.000 claims description 2
- 235000013828 hydroxypropyl starch Nutrition 0.000 claims description 2
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims description 2
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims description 2
- NLYAJNPCOHFWQQ-UHFFFAOYSA-N kaolin Chemical compound O.O.O=[Al]O[Si](=O)O[Si](=O)O[Al]=O NLYAJNPCOHFWQQ-UHFFFAOYSA-N 0.000 claims description 2
- 235000019691 monocalcium phosphate Nutrition 0.000 claims description 2
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 2
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 2
- 239000001254 oxidized starch Substances 0.000 claims description 2
- 229910052625 palygorskite Inorganic materials 0.000 claims description 2
- 229920000058 polyacrylate Polymers 0.000 claims description 2
- 229920000151 polyglycol Polymers 0.000 claims description 2
- 239000010695 polyglycol Substances 0.000 claims description 2
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims description 2
- 229920000053 polysorbate 80 Polymers 0.000 claims description 2
- 229920002689 polyvinyl acetate Polymers 0.000 claims description 2
- 239000011118 polyvinyl acetate Substances 0.000 claims description 2
- 239000001267 polyvinylpyrrolidone Substances 0.000 claims description 2
- 229920000036 polyvinylpyrrolidone Polymers 0.000 claims description 2
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 claims description 2
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims description 2
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims description 2
- 239000011734 sodium Substances 0.000 claims description 2
- 229910052708 sodium Inorganic materials 0.000 claims description 2
- 235000010413 sodium alginate Nutrition 0.000 claims description 2
- 239000000661 sodium alginate Substances 0.000 claims description 2
- 229940005550 sodium alginate Drugs 0.000 claims description 2
- 235000019812 sodium carboxymethyl cellulose Nutrition 0.000 claims description 2
- 229920001027 sodium carboxymethylcellulose Polymers 0.000 claims description 2
- APSBXTVYXVQYAB-UHFFFAOYSA-M sodium docusate Chemical group [Na+].CCCCC(CC)COC(=O)CC(S([O-])(=O)=O)C(=O)OCC(CC)CCCC APSBXTVYXVQYAB-UHFFFAOYSA-M 0.000 claims description 2
- 229940029258 sodium glycinate Drugs 0.000 claims description 2
- 229940074404 sodium succinate Drugs 0.000 claims description 2
- ZDQYSKICYIVCPN-UHFFFAOYSA-L sodium succinate (anhydrous) Chemical compound [Na+].[Na+].[O-]C(=O)CCC([O-])=O ZDQYSKICYIVCPN-UHFFFAOYSA-L 0.000 claims description 2
- PYODKQIVQIVELM-UHFFFAOYSA-M sodium;2,3-bis(2-methylpropyl)naphthalene-1-sulfonate Chemical compound [Na+].C1=CC=C2C(S([O-])(=O)=O)=C(CC(C)C)C(CC(C)C)=CC2=C1 PYODKQIVQIVELM-UHFFFAOYSA-M 0.000 claims description 2
- WUWHFEHKUQVYLF-UHFFFAOYSA-M sodium;2-aminoacetate Chemical compound [Na+].NCC([O-])=O WUWHFEHKUQVYLF-UHFFFAOYSA-M 0.000 claims description 2
- KDYFGRWQOYBRFD-UHFFFAOYSA-L succinate(2-) Chemical compound [O-]C(=O)CCC([O-])=O KDYFGRWQOYBRFD-UHFFFAOYSA-L 0.000 claims description 2
- 229940095064 tartrate Drugs 0.000 claims description 2
- OKUCEQDKBKYEJY-UHFFFAOYSA-N tert-butyl 3-(methylamino)pyrrolidine-1-carboxylate Chemical compound CNC1CCN(C(=O)OC(C)(C)C)C1 OKUCEQDKBKYEJY-UHFFFAOYSA-N 0.000 claims description 2
- 239000011573 trace mineral Substances 0.000 claims description 2
- 235000013619 trace mineral Nutrition 0.000 claims description 2
- 235000010487 tragacanth Nutrition 0.000 claims description 2
- 239000000196 tragacanth Substances 0.000 claims description 2
- 229940116362 tragacanth Drugs 0.000 claims description 2
- 229940074410 trehalose Drugs 0.000 claims description 2
- 229940117013 triethanolamine oleate Drugs 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 39
- 239000000575 pesticide Substances 0.000 abstract description 14
- 239000004480 active ingredient Substances 0.000 abstract description 5
- 239000000419 plant extract Substances 0.000 abstract 1
- 239000007921 spray Substances 0.000 abstract 1
- 230000004083 survival effect Effects 0.000 abstract 1
- 230000012010 growth Effects 0.000 description 14
- 244000061456 Solanum tuberosum Species 0.000 description 12
- 235000002595 Solanum tuberosum Nutrition 0.000 description 12
- 240000008042 Zea mays Species 0.000 description 12
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 12
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 12
- 235000005822 corn Nutrition 0.000 description 12
- 238000012360 testing method Methods 0.000 description 12
- 238000011161 development Methods 0.000 description 11
- 230000018109 developmental process Effects 0.000 description 11
- 240000008067 Cucumis sativus Species 0.000 description 10
- 244000052616 bacterial pathogen Species 0.000 description 10
- 239000000126 substance Substances 0.000 description 10
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 description 8
- 230000002265 prevention Effects 0.000 description 8
- 230000001954 sterilising effect Effects 0.000 description 8
- 210000002421 cell wall Anatomy 0.000 description 7
- 239000002207 metabolite Substances 0.000 description 7
- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 description 6
- 241000219112 Cucumis Species 0.000 description 6
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 6
- 241000223218 Fusarium Species 0.000 description 6
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 6
- 240000003768 Solanum lycopersicum Species 0.000 description 6
- 230000009471 action Effects 0.000 description 6
- 230000000844 anti-bacterial effect Effects 0.000 description 6
- 230000000295 complement effect Effects 0.000 description 6
- 230000001276 controlling effect Effects 0.000 description 6
- 244000053095 fungal pathogen Species 0.000 description 6
- 230000007246 mechanism Effects 0.000 description 6
- 238000001228 spectrum Methods 0.000 description 6
- 239000004563 wettable powder Substances 0.000 description 6
- 241000193830 Bacillus <bacterium> Species 0.000 description 5
- 240000007124 Brassica oleracea Species 0.000 description 5
- 235000003899 Brassica oleracea var acephala Nutrition 0.000 description 5
- 235000011301 Brassica oleracea var capitata Nutrition 0.000 description 5
- 235000001169 Brassica oleracea var oleracea Nutrition 0.000 description 5
- 235000002566 Capsicum Nutrition 0.000 description 5
- 241000223221 Fusarium oxysporum Species 0.000 description 5
- 241000244206 Nematoda Species 0.000 description 5
- 241000813090 Rhizoctonia solani Species 0.000 description 5
- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 description 5
- 239000013543 active substance Substances 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 5
- 230000002401 inhibitory effect Effects 0.000 description 5
- 230000002503 metabolic effect Effects 0.000 description 5
- 239000008223 sterile water Substances 0.000 description 5
- 240000008574 Capsicum frutescens Species 0.000 description 4
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 description 4
- 102000004190 Enzymes Human genes 0.000 description 4
- 108090000790 Enzymes Proteins 0.000 description 4
- 229940024606 amino acid Drugs 0.000 description 4
- 239000001390 capsicum minimum Substances 0.000 description 4
- 239000006013 carbendazim Substances 0.000 description 4
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 description 4
- 230000007547 defect Effects 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 230000001717 pathogenic effect Effects 0.000 description 4
- 238000009331 sowing Methods 0.000 description 4
- 238000003756 stirring Methods 0.000 description 4
- 239000000375 suspending agent Substances 0.000 description 4
- 239000000725 suspension Substances 0.000 description 4
- 241000255777 Lepidoptera Species 0.000 description 3
- 241000588701 Pectobacterium carotovorum Species 0.000 description 3
- RWRDLPDLKQPQOW-UHFFFAOYSA-N Pyrrolidine Chemical compound C1CCNC1 RWRDLPDLKQPQOW-UHFFFAOYSA-N 0.000 description 3
- 239000005708 Sodium hypochlorite Substances 0.000 description 3
- 150000001413 amino acids Chemical class 0.000 description 3
- 210000000170 cell membrane Anatomy 0.000 description 3
- 230000006378 damage Effects 0.000 description 3
- 230000000593 degrading effect Effects 0.000 description 3
- 230000007613 environmental effect Effects 0.000 description 3
- 239000003337 fertilizer Substances 0.000 description 3
- 230000035784 germination Effects 0.000 description 3
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 description 3
- 230000002147 killing effect Effects 0.000 description 3
- 239000011159 matrix material Substances 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 230000008635 plant growth Effects 0.000 description 3
- 230000007226 seed germination Effects 0.000 description 3
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 3
- 239000002689 soil Substances 0.000 description 3
- 238000005406 washing Methods 0.000 description 3
- 241000213004 Alternaria solani Species 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 235000009849 Cucumis sativus Nutrition 0.000 description 2
- 241000233866 Fungi Species 0.000 description 2
- FAIXYKHYOGVFKA-UHFFFAOYSA-N Kinetin Natural products N=1C=NC=2N=CNC=2C=1N(C)C1=CC=CO1 FAIXYKHYOGVFKA-UHFFFAOYSA-N 0.000 description 2
- 241000243785 Meloidogyne javanica Species 0.000 description 2
- 241000589516 Pseudomonas Species 0.000 description 2
- JUJWROOIHBZHMG-UHFFFAOYSA-N Pyridine Chemical compound C1=CC=NC=C1 JUJWROOIHBZHMG-UHFFFAOYSA-N 0.000 description 2
- 241000223259 Trichoderma Species 0.000 description 2
- 244000000005 bacterial plant pathogen Species 0.000 description 2
- 238000004364 calculation method Methods 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 239000004062 cytokinin Substances 0.000 description 2
- UQHKFADEQIVWID-UHFFFAOYSA-N cytokinin Natural products C1=NC=2C(NCC=C(CO)C)=NC=NC=2N1C1CC(O)C(CO)O1 UQHKFADEQIVWID-UHFFFAOYSA-N 0.000 description 2
- QRMZSPFSDQBLIX-UHFFFAOYSA-N homovanillic acid Chemical compound COC1=CC(CC(O)=O)=CC=C1O QRMZSPFSDQBLIX-UHFFFAOYSA-N 0.000 description 2
- 239000003864 humus Substances 0.000 description 2
- 230000000749 insecticidal effect Effects 0.000 description 2
- 238000011835 investigation Methods 0.000 description 2
- QANMHLXAZMSUEX-UHFFFAOYSA-N kinetin Chemical compound N=1C=NC=2N=CNC=2C=1NCC1=CC=CO1 QANMHLXAZMSUEX-UHFFFAOYSA-N 0.000 description 2
- 229960001669 kinetin Drugs 0.000 description 2
- 244000144972 livestock Species 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 239000005648 plant growth regulator Substances 0.000 description 2
- 239000013641 positive control Substances 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 230000002829 reductive effect Effects 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 230000002195 synergetic effect Effects 0.000 description 2
- 239000010455 vermiculite Substances 0.000 description 2
- 229910052902 vermiculite Inorganic materials 0.000 description 2
- 235000019354 vermiculite Nutrition 0.000 description 2
- WTXHFWYAETTXNF-XZWHSSHBSA-N (+)-Isotetrandrine Natural products O(C)c1c(OC)cc2c3[C@H](N(C)CC2)Cc2cc(c(OC)cc2)Oc2cc(ccc2)C[C@@H]2N(C)CCc4c2cc(c(OC)c4)Oc13 WTXHFWYAETTXNF-XZWHSSHBSA-N 0.000 description 1
- DRBARRGCABOUIE-UHFFFAOYSA-N 2,3-dihydroxy-3-phenylprop-2-enoic acid Chemical compound OC(=O)C(O)=C(O)C1=CC=CC=C1 DRBARRGCABOUIE-UHFFFAOYSA-N 0.000 description 1
- JAHNSTQSQJOJLO-UHFFFAOYSA-N 2-(3-fluorophenyl)-1h-imidazole Chemical compound FC1=CC=CC(C=2NC=CN=2)=C1 JAHNSTQSQJOJLO-UHFFFAOYSA-N 0.000 description 1
- CIBKSMZEVHTQLG-UHFFFAOYSA-N 5-Ethyl-2,3-dimethylpyrazine Chemical compound CCC1=CN=C(C)C(C)=N1 CIBKSMZEVHTQLG-UHFFFAOYSA-N 0.000 description 1
- 241000186361 Actinobacteria <class> Species 0.000 description 1
- 229930195730 Aflatoxin Natural products 0.000 description 1
- XWIYFDMXXLINPU-UHFFFAOYSA-N Aflatoxin G Chemical compound O=C1OCCC2=C1C(=O)OC1=C2C(OC)=CC2=C1C1C=COC1O2 XWIYFDMXXLINPU-UHFFFAOYSA-N 0.000 description 1
- 241001124076 Aphididae Species 0.000 description 1
- 241000228197 Aspergillus flavus Species 0.000 description 1
- 229930192334 Auxin Natural products 0.000 description 1
- 108010062877 Bacteriocins Proteins 0.000 description 1
- XPCFTKFZXHTYIP-PMACEKPBSA-N Benazepril Chemical compound C([C@@H](C(=O)OCC)N[C@@H]1C(N(CC(O)=O)C2=CC=CC=C2CC1)=O)CC1=CC=CC=C1 XPCFTKFZXHTYIP-PMACEKPBSA-N 0.000 description 1
- 235000016068 Berberis vulgaris Nutrition 0.000 description 1
- 241000335053 Beta vulgaris Species 0.000 description 1
- 241000123650 Botrytis cinerea Species 0.000 description 1
- 208000003643 Callosities Diseases 0.000 description 1
- 241001290235 Ceratobasidium cereale Species 0.000 description 1
- 102000012286 Chitinases Human genes 0.000 description 1
- 108010022172 Chitinases Proteins 0.000 description 1
- 241000186216 Corynebacterium Species 0.000 description 1
- 241000289763 Dasygaster padockina Species 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 241000221785 Erysiphales Species 0.000 description 1
- 206010051814 Eschar Diseases 0.000 description 1
- 240000006499 Flammulina velutipes Species 0.000 description 1
- 235000016640 Flammulina velutipes Nutrition 0.000 description 1
- 241000526118 Fusarium solani f. radicicola Species 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 206010020649 Hyperkeratosis Diseases 0.000 description 1
- 108010052285 Membrane Proteins Proteins 0.000 description 1
- 102000018697 Membrane Proteins Human genes 0.000 description 1
- 241001477931 Mythimna unipuncta Species 0.000 description 1
- SYDUHMOSZYKITO-UHFFFAOYSA-N N(=O)C1(SCCN1)C(=O)O Chemical compound N(=O)C1(SCCN1)C(=O)O SYDUHMOSZYKITO-UHFFFAOYSA-N 0.000 description 1
- 241001503951 Phoma Species 0.000 description 1
- 241000233629 Phytophthora parasitica Species 0.000 description 1
- 231100000674 Phytotoxicity Toxicity 0.000 description 1
- 241000758706 Piperaceae Species 0.000 description 1
- 241000500437 Plutella xylostella Species 0.000 description 1
- 241000589623 Pseudomonas syringae pv. syringae Species 0.000 description 1
- 206010039509 Scab Diseases 0.000 description 1
- 241001558929 Sclerotium <basidiomycota> Species 0.000 description 1
- 108010013296 Sericins Proteins 0.000 description 1
- 235000021355 Stearic acid Nutrition 0.000 description 1
- 241000187747 Streptomyces Species 0.000 description 1
- 241000499912 Trichoderma reesei Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 244000098338 Triticum aestivum Species 0.000 description 1
- 235000010726 Vigna sinensis Nutrition 0.000 description 1
- 244000042314 Vigna unguiculata Species 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 231100000215 acute (single dose) toxicity testing Toxicity 0.000 description 1
- 238000011047 acute toxicity test Methods 0.000 description 1
- 239000005409 aflatoxin Substances 0.000 description 1
- VQKFNUFAXTZWDK-UHFFFAOYSA-N alpha-methylfuran Natural products CC1=CC=CO1 VQKFNUFAXTZWDK-UHFFFAOYSA-N 0.000 description 1
- 125000000539 amino acid group Chemical group 0.000 description 1
- 239000005557 antagonist Substances 0.000 description 1
- 239000002363 auxin Substances 0.000 description 1
- 229960004530 benazepril Drugs 0.000 description 1
- 239000012681 biocontrol agent Substances 0.000 description 1
- 239000003124 biologic agent Substances 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 230000000853 biopesticidal effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- RHDGNLCLDBVESU-UHFFFAOYSA-N but-3-en-4-olide Chemical compound O=C1CC=CO1 RHDGNLCLDBVESU-UHFFFAOYSA-N 0.000 description 1
- 210000002390 cell membrane structure Anatomy 0.000 description 1
- 230000002860 competitive effect Effects 0.000 description 1
- 230000000536 complexating effect Effects 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 238000010924 continuous production Methods 0.000 description 1
- 238000013270 controlled release Methods 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 230000009034 developmental inhibition Effects 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- SIUKXCMDYPYCLH-UHFFFAOYSA-N dihydroxycinnamic acid Natural products OC(=O)C=CC1=CC=CC(O)=C1O SIUKXCMDYPYCLH-UHFFFAOYSA-N 0.000 description 1
- 230000006806 disease prevention Effects 0.000 description 1
- 239000002552 dosage form Substances 0.000 description 1
- 230000000857 drug effect Effects 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 231100000333 eschar Toxicity 0.000 description 1
- AEOCXXJPGCBFJA-UHFFFAOYSA-N ethionamide Chemical compound CCC1=CC(C(N)=S)=CC=N1 AEOCXXJPGCBFJA-UHFFFAOYSA-N 0.000 description 1
- 229960002001 ethionamide Drugs 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 239000007888 film coating Substances 0.000 description 1
- 238000009501 film coating Methods 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 230000009036 growth inhibition Effects 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 239000003630 growth substance Substances 0.000 description 1
- LHGVFZTZFXWLCP-UHFFFAOYSA-N guaiacol Chemical compound COC1=CC=CC=C1O LHGVFZTZFXWLCP-UHFFFAOYSA-N 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000003617 indole-3-acetic acid Substances 0.000 description 1
- 150000002475 indoles Chemical class 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- WVTKBKWTSCPRNU-XZWHSSHBSA-N isotetrandrine Chemical compound C([C@H]1C=2C=C(C(=CC=2CCN1C)OC)O1)C(C=C2)=CC=C2OC(=C2)C(OC)=CC=C2C[C@H]2N(C)CCC3=CC(OC)=C(OC)C1=C23 WVTKBKWTSCPRNU-XZWHSSHBSA-N 0.000 description 1
- 230000002045 lasting effect Effects 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 230000003859 lipid peroxidation Effects 0.000 description 1
- 230000005923 long-lasting effect Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- HZVOZRGWRWCICA-UHFFFAOYSA-N methanediyl Chemical compound [CH2] HZVOZRGWRWCICA-UHFFFAOYSA-N 0.000 description 1
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 1
- LVHBHZANLOWSRM-UHFFFAOYSA-N methylenebutanedioic acid Natural products OC(=O)CC(=C)C(O)=O LVHBHZANLOWSRM-UHFFFAOYSA-N 0.000 description 1
- ZIYVHBGGAOATLY-UHFFFAOYSA-N methylmalonic acid Chemical compound OC(=O)C(C)C(O)=O ZIYVHBGGAOATLY-UHFFFAOYSA-N 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 230000003020 moisturizing effect Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- QIQXTHQIDYTFRH-UHFFFAOYSA-N octadecanoic acid Chemical compound CCCCCCCCCCCCCCCCCC(O)=O QIQXTHQIDYTFRH-UHFFFAOYSA-N 0.000 description 1
- OQCDKBAXFALNLD-UHFFFAOYSA-N octadecanoic acid Natural products CCCCCCCC(C)CCCCCCCCC(O)=O OQCDKBAXFALNLD-UHFFFAOYSA-N 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 239000003895 organic fertilizer Substances 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 230000010412 perfusion Effects 0.000 description 1
- 239000003375 plant hormone Substances 0.000 description 1
- 235000010958 polyglycerol polyricinoleate Nutrition 0.000 description 1
- 235000012015 potatoes Nutrition 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 238000001243 protein synthesis Methods 0.000 description 1
- UMJSCPRVCHMLSP-UHFFFAOYSA-N pyridine Natural products COC1=CC=CN=C1 UMJSCPRVCHMLSP-UHFFFAOYSA-N 0.000 description 1
- WVTKBKWTSCPRNU-UHFFFAOYSA-N rac-Tetrandrin Natural products O1C(C(=CC=2CCN3C)OC)=CC=2C3CC(C=C2)=CC=C2OC(=C2)C(OC)=CC=C2CC2N(C)CCC3=CC(OC)=C(OC)C1=C23 WVTKBKWTSCPRNU-UHFFFAOYSA-N 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- WBHHMMIMDMUBKC-XLNAKTSKSA-N ricinelaidic acid Chemical compound CCCCCC[C@@H](O)C\C=C\CCCCCCCC(O)=O WBHHMMIMDMUBKC-XLNAKTSKSA-N 0.000 description 1
- 229960003656 ricinoleic acid Drugs 0.000 description 1
- FEUQNCSVHBHROZ-UHFFFAOYSA-N ricinoleic acid Natural products CCCCCCC(O[Si](C)(C)C)CC=CCCCCCCCC(=O)OC FEUQNCSVHBHROZ-UHFFFAOYSA-N 0.000 description 1
- 229940080817 rotenone Drugs 0.000 description 1
- JUVIOZPCNVVQFO-UHFFFAOYSA-N rotenone Natural products O1C2=C3CC(C(C)=C)OC3=CC=C2C(=O)C2C1COC1=C2C=C(OC)C(OC)=C1 JUVIOZPCNVVQFO-UHFFFAOYSA-N 0.000 description 1
- 238000011076 safety test Methods 0.000 description 1
- 230000035040 seed growth Effects 0.000 description 1
- 230000004763 spore germination Effects 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 238000013268 sustained release Methods 0.000 description 1
- 239000012730 sustained-release form Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 230000014616 translation Effects 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/22—Bacillus
- A01N63/23—B. thuringiensis
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/06—Coating or dressing seed
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/22—Bacillus
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P1/00—Disinfectants; Antimicrobial compounds or mixtures thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P21/00—Plant growth regulators
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P3/00—Fungicides
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P5/00—Nematocides
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P7/00—Arthropodicides
- A01P7/04—Insecticides
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Wood Science & Technology (AREA)
- Plant Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Chemical & Material Sciences (AREA)
- General Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Dentistry (AREA)
- Agronomy & Crop Science (AREA)
- Biotechnology (AREA)
- Virology (AREA)
- Mycology (AREA)
- Insects & Arthropods (AREA)
- Botany (AREA)
- Soil Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention provides a composite biocontrol strain seed treating agent, which is solid powder formed by 80-90% of effective active components and 10-20% of auxiliary materials. The effective active components are concentrated and solidified matters of the equal proportion composite bacterial fermentation liquid of bacillus subtilis, bacillus cereus and bacillus thuringiensis. The invention mixes and sprays the fermentation concentrated solution of a plurality of strains with a carrier to obtain a concentrated solidified compound bacterial fermentation solution; mixing the plant extract with auxiliary materials to obtain the plant-controlled pesticide composition, wherein the plant-controlled pesticide composition is used for controlling plant diseases and insect pests in seeds of vegetables and crop seeds and in seedling stage of the plant-controlled pesticide composition; the seed treatment agent has high content of active ingredients, can effectively prevent and control various seeds and plant diseases and insect pests in the seedling stage, has a control effect of 61.44-83.65%, improves the seed emergence rate and survival rate, and has an average emergence rate of more than 93.58%.
Description
Technical Field
The invention belongs to the technical field of biological pesticides, and in particular relates to a multi-effect biological seed treatment agent taking multiple strains of biological control microorganisms and metabolic products as main active ingredients, a preparation method and application.
Background
The seed treatment is one of the economic and simple methods in crop pest control, the agent for treating the seeds is a seed treatment agent, the seed treatment agent can be divided into a seed dressing agent and a seed coating agent, the seed dressing agent belongs to the existing seed treatment agent, and the seed treatment agent is used before sowing seeds, so that the outside of the seeds is provided with pesticide active ingredients, and the effects of killing and resisting diseases are achieved. However, the general seed dressing agent has low requirements on the preparation, so that the agent has poor adhesiveness to the seeds after the seeds are treated, is easy to fall off, has short drug effect period, is used in the prior art, increases the dosage and operation procedures, has unsatisfactory application effect, and needs further innovation and improvement in the aspect of preparation to adapt to the development requirements of new agriculture. The seed coating agent belongs to a pre-combined seed treatment agent, the stability of a medicinal film after coating the seeds is good, the effective components of the seed coating agent are not easy to diffuse to the periphery rapidly after coating the seeds, the lasting period is long, the seed coating agent is closely attached to the seeds, the medicinal efficacy is concentrated, the bioavailability is high, plant diseases and insect pests from sprouting to a seedling stage can be effectively prevented and controlled, the smooth sprouting of the seeds is ensured, the healthy growth and development of seedlings are promoted, the coated seeds can be stored for a long time, the use is convenient, the seed coating agent is suitable for mechanical mass production, and the seed coating agent can be used as a novel seed coating agent due to the good film forming property, so the seed coating agent becomes an excellent type of the seed treatment agent and is paid attention to.
Seed treatment technology developed to a new level after the 80 s of the last century. Firstly, the development of new varieties of efficient, economical and safe pesticides is continuously used for replacing the traditional seed treatment agent. Secondly, the development and application of new dosage forms such as suspending agents, suspoemulsions, various solid preparations and the like lead the seed treatment technology to reach a new level. Thirdly, research and development of film coating seed technology promotes rapid development of seed treatment towards industrialization, mechanization and commercialization. The fourth modern seed treatment agent should have controlled and sustained release effect, and has long lasting period, and is safe to natural enemies, people and livestock and environment. Fifth, with the continuous development of biotechnology and molecular biology, studies on seed treatment using biological agents have been widely conducted, and the development of seed treatment agents is also a future development direction. The biological seed treating agent is one kind of green seed treating agent developed with biological fertilizer and biological pesticide as main active components. The seed treating agent with nitrogen fixing bacteria, phosphate decomposing bacteria, potassium decomposing bacteria, organic matter and other functional biological fertilizer bacteria and nutrients as active components is prepared through the process of producing fertilizer to promote plant to absorb nutrients, improving plant's own nourishing capacity, and has weak disease and pest preventing and controlling capacity. Therefore, seed treatments using biopesticides as a main active ingredient are more the mainstream of biological seed treatments in the future.
Biological seed treatment agents which are applied and developed at present and take biological pesticides as main active components, most of which are biocontrol bacteria single-strain seed coating agents such as bacillus, trichoderma and the like, such as: coating seed agents such as soybean, corn and the like prepared by trichoderma in the United states; thompson et al prepared beet coated seed agents for the control of saprolegniasis using antagonists and metabolites thereof; patent ZSB biological seed coating agent developed in Zhejiang has been widely used for various crops; the biological seed coating agent developed by taking trichoderma reesei REMI mutant strain fermentation liquor as an active ingredient and the like has a relative control effect of 70-75% on cucumber seed powdery mildew, and the like. And a small part of the composite seed coating agent compounded by biocontrol bacteria or biocontrol bacteria and chemical pesticides, such as: the BA biological seed coating agent which is developed by Fangxin and is compounded by bacteria and actinomycetes has better effect when being applied to wheat, corn, tomatoes and cucumbers; corn seed coating agent developed by Cesare and formed by compounding aflatoxin-free aspergillus flavus strain and two traditional pesticides can reduce the content of aflatoxin in corn kernels and protect seed growth; the Rocha et al compound and grind PGPR pseudomonas with arbuscular mycorrhizal fungi into a microbial seed coating agent, so that the yield of cowpea under low-input agricultural conditions is improved, and the like. The biocontrol seed treating agent overcomes the defects of chemical pesticide seed treating agents and has better application effect. However, the preparation of single strain has the defects of narrow prevention and control spectrum, single antibacterial mechanism, weak competitive power and unstable practical application effect, and the biological control bacteria or the compound preparation of the biological control bacteria and chemical pesticides cannot give consideration to the harm of diseases and insects, and the active bacteria are easy to be influenced by the environment, the preparation has short storage period, the chemical pesticides are brought into the preparation, the safety of the preparation is influenced, and the biological seed treatment agent in the real sense cannot be calculated.
In view of the defects of the existing seed treatment agents and the unique advantages of biological seed treatment agents, the invention develops the composite biocontrol bacterium solid seed coating agent by taking a plurality of biocontrol bacterium groups with strong stress resistance, biocontrol spectrum and action mechanism and complementary metabolic products thereof as active components, and the composite biocontrol bacterium solid seed coating agent has the advantages of safety, good control effect and stability, wide control spectrum, wide application range and longer shelf life.
Disclosure of Invention
The invention aims at providing a composite biocontrol strain seed treating agent with a plurality of biocontrol strain groups and metabolites thereof as main active components.
The second purpose of the invention is to provide a preparation method of the composite biocontrol strain seed treating agent, and the obtained solid seed treating agent is not easily affected by environmental factors, is relatively stable, has longer shelf life and is convenient to transport.
The invention further aims to provide an application technology of the composite biocontrol seed treating agent, which is used for preventing and controlling seed with shells of cucumbers, melons, tomatoes, peppers and the like, tuber seeds of potatoes and the like, crop seeds of corns and the like and plant diseases and insect pests in seedling stage of the crop seeds.
The aim of the invention is achieved by the following technical scheme:
the seed treating agent is prepared from the following components in percentage by weight: 80 to 90 percent of effective active components, 1 to 8.0 percent of film forming agent, 2.0 to 5.0 percent of wetting dispersant, 0.01 to 1.0 percent of stabilizer, 5.50 to 6.89 percent of filling agent and 0.1 to 0.5 percent of warning color; the effective active component is concentrated and solidified matters of the equal proportion composite bacterial fermentation liquid of bacillus subtilis and bacillus cereus; the bacillus subtilis is bacillus subtilis Bacillus subtilis with a preservation number of CGMCC No.24484; the Bacillus cereus is Bacillus cereus with a preservation number of CGMCC No.24485; the bacillus thuringiensis is bacillus thuringiensis Bacillus thuringiensis and has a preservation number of CGMCC No.24483.
The film forming agent is one or a mixture of several of sodium carboxymethyl starch, soluble starch, phosphorylated starch-oxidized starch acetate, sodium carboxymethyl cellulose, hydroxypropyl methyl cellulose, sodium alginate, PVA17-88, PVA05-88, polyvinyl acetate, polyacrylamide, polyvinylpyrrolidone, polyacrylate, acacia, tragacanth, oxidized hydroxypropyl starch and calcium silicate in any ratio.
The wetting dispersant is one or a mixture of several of triethanolamine oleate, morwetEFW, ethylan1008W, ethylanNS-500LQ, dodecyl sulfonate, dioctyl sodium succinate sulfonate, span80, tween-80, morwet D-425, morwet D-500, lignin sulfonate, nekal BX, dispersinf AgentNNO, alkyl succinate sulfonate, dibutyl naphthalene sulfonate and the like.
The stabilizer is one or a mixture of several of magnesium aluminum silicate, aluminum stearate, hydrogen citrate, tartrate, magnesium succinate, sodium glycinate, organosilicon and fatty alcohol polyglycol ether in any ratio.
The filler is one or a mixture of several of bentonite, diatomite, attapulgite, calcium carbonate, dextrin, kaolin, white carbon black, agar, monopotassium phosphate, calcium superphosphate, ferrous sulfate, trace elements and the like in any ratio.
The warning color is one of weak acid dyes such as black T, complexing yellow GR, rose essence and the like.
The preparation method of the composite biocontrol strain seed treating agent comprises the following specific steps: firstly, mixing the Bacillus subtilis Bacillus subtilis with the preservation number of CGMCC No.24484 and Bacillus cereus with the preservation numbers of CGMCC No.24485 and Bacillus thuringiensis Bacillus thuringiensis and the fermentation concentrated solution with the preservation number of CGMCC No.24483 with a superfine crushed carrier, and spray-drying at the temperature below 70 ℃ to obtain a concentrated condensate of the composite bacterial fermentation solution; secondly, respectively superfine grinding all auxiliary materials, and fully and uniformly mixing 80% -90% of concentrated and solidified compound bacteria fermentation liquor, 0.01% -1.0% of stabilizer and 5.50% -6.89% of filler for 20 min-30 min; finally, adding 1.0 to 8.0 percent of film forming agent and 2.0 to 5.0 percent of wetting dispersant, uniformly mixing for 5 to 0min, adding 0.1 to 0.5 percent of warning color, and uniformly mixing for 30 to 40min; sieving with 1340 mesh sieve, immediately placing in clean environment with temperature of 28-30deg.C and more than 10 ten thousand grades, drying for 5-48 h to obtain composite biological control bacteria solid seed treating agent with water content less than 5%; wherein the carrier is one or more of potassium humate, peat, alginate, urea, polyacrylamide, trehalose, chitosan, sodium glutamate, maltodextrin, milk powder, phosphate and the like.
The application of the composite biocontrol strain seed treating agent is used for preventing and controlling plant diseases and insect pests in seeds of vegetables and crop seeds and at the seedling stage of the seeds; the composite biocontrol strain seed treating agent is coated or mixed with seeds, and is diluted in the prior art; the seed coating can be diluted 100-500 times, and can be mechanically coated or manually coated, and the drying temperature is controlled below 35 ℃; seed dressing, diluting 300-1000 times, and seed dressing.
The composite biocontrol strain seed treating agent provided by the invention has the advantages that:
1. the content of the effective components is high, the action mechanisms of the biological control are complementary, and the application effect is good. The composite biocontrol strain seed treating agent provided by the invention mainly comprises three biocontrol strains of different genera, wherein the three biocontrol strains have complementary action mechanisms and action targets, the three biocontrol strains respectively have complementary cooperation through destroying cell wall and cell membrane structures and stability, inhibiting protein synthesis and destroying the activity of a nematode and lepidoptera pest organism, the biocontrol activity is improved, the stress resistance is enhanced, the control effect on seed and seedling diseases and insect pests is 61.44% -83.65%, and the bacillus subtilis growth metabolism can also produce auxin, so that seed germination and seedling growth are promoted, and the emergence rate is improved by 10.21% -12.75%.
2. Broad control spectrum and wide application range. The 3 strains of biocontrol bacteria used in the composite biocontrol strain seed treating agent provided by the invention have different action mechanisms and metabolites to form flora, make up for the advantages and complement the advantages, widen the control spectrum, can be used for having stronger activity on various pathogenic bacteria and fungi pathogenic bacteria, nematodes and lepidoptera larvae, and the auxiliary materials of the preparation are selected to give consideration to the functions and synergistic effects of the auxiliary materials, so that the application range of the composite biocontrol strain seed treating agent is widened. Can be used for seeds of various plants such as cucumber, melon, potato, corn and the like, and belongs to a universal seed treatment agent.
3. The solid preparation has good stability. The composite biocontrol strain seed treating agent provided by the invention is a biological wettable powder seed treating agent, the main active components of the composite biocontrol strain seed treating agent are a plurality of biocontrol strains and a fermentation product solid preparation thereof, and the three biocontrol strains are not easily influenced by environmental factors in a dry state, so that the composite biocontrol strain seed treating agent is relatively stable, the shelf life is prolonged, and the transportation is convenient.
4. Safety and environmental protection. The main active components of the invention are a plurality of biological control microorganisms from the nature, the acute toxicity test proves that the invention is a nontoxic substance, and the plant safety test shows that the invention has no phytotoxicity, and belongs to a truly green environment-friendly pesticide which is safe to human, livestock and environment.
5. The production and application cost is low. The invention achieves the aim of effectively preventing and controlling plant diseases and insect pests in the seedling stage by treating plant seeds, aims at prevention, fully plays the advantages of small prevention and early prevention of the biocontrol agent, has small dosage and good effect. On the other hand, the bio-control microorganism fermentation solidified material serving as the main active component has the advantages of abundant sources of main production raw materials, lower price, no resource limitation, continuous production, relatively simple production process and low cost.
Detailed Description
The present invention will be further described in detail below with respect to the principles and implementations, to provide a more complete understanding of the present invention to those skilled in the art, but are not to be construed as limiting the present invention in any way.
The principle of the invention is first explained as follows:
the main effective active substances of the invention are composed of three strains of biocontrol bacteria of different genus and metabolites thereof, and have complementary synergistic effect in application due to different action mechanisms and action targets, thereby achieving the composite targets of broad spectrum, high efficiency and wider application range.
Bacillus subtilisBacillus subtilisThe metabolic active substances contain substances such as indoles, cytokinins, plant hormones, sugar, various amino acids and the like which promote the growth and development of plants, and have obvious growth promoting effect on protected plants. Octadecanoic acid, itaconic acid, sericin, benazepril, hydroxylbenzovidone, hydroxylburan, furanone, methylfuran aldehyde, nitrosothiazolidinecarboxylic acid and other antibacterial substances. The method has the advantages that the pathogenic fungus cell wall chitinase and related protective enzymes are destroyed to synthesize and promote lipid peroxidation of pathogenic fungus cells, and the cell wall and cell membrane stability of the pathogenic fungus are destroyed, so that the cell protoplasm of the pathogenic fungus is leaked and killed, meanwhile, the activity of the pathogenic fungus cell wall degrading enzyme is inhibited, the infection capability of the pathogenic fungus cell wall degrading enzyme on host plants is reduced, and the aim of inhibiting plant pathogenic bacteria such as Botrytis cinerea, fusarium oxysporum, fusarium putrescens, rhizoctonia solani, pseudomonas pathogenic bacteria, rhizoctonia cerealis, phoma tix and the like is achieved.
Bacillus cereusBacillus cereusThe metabolically active substances contain bacteriocins such as isotetrandrine, ethionamide, methylmalonic acid, methoxyphenol, ricinoleic acid and the like and organic acid antibacterial substances. Bacillus cereusBacillus cereusStrong fertility, rapid diffusion, and through competing with pathogenic bacteriaThe cell membrane is seriously damaged by competing for space occupation and inhibiting the synthesis of pathogenic bacteria cell membrane protein so as to achieve the aim of inhibiting plant pathogenic bacteria such as streptomyces scab, solanacearum, soft-rot Erwinia carotovora soft-rot pathogenic type, soft-rot Erwinia carotovora black-rot pathogenic type, corynebacterium circulans, pseudomonas syringae pathogenic type, eschar, fusarium oxysporum, alternaria solani and the like. In addition, the fermentation metabolites of the plant growth regulator also comprise plant growth regulators such as indoleacetic acid, cytokinin, amino acid and the like, so that the growth and development of the protected plants are promoted.
Bacillus thuringiensisBacillus thuringiensisThe metabolic active substances contain dihydroxycinnamic acid, homovanillic acid, aminobutyral, ethyldimethyl pyrazine, methyl deoxyjirimycin, pyridine base, peptide, compound amino acid, rotenone, carbene dehydropiperazine, tetrahydropyrrole and other insecticidal antibacterial compounds, and the metabolic active substances have the effects of killing or inhibiting lepidoptera pests such as armyworms and plutella xylostella, aphid and other ptera pests and crop nematodes such as root-knot nematodes and root-rot nematodes through the effects of stomach toxicity, contact killing and growth and development inhibition. In addition, the metabolites of the plant growth regulating substances such as single amino acid groups, plant kinetin and the like can promote the health of the protected plants and enhance the disease resistance and insect resistance of the plants.
Two strains of the three biocontrol bacteria cooperatively attack cell membranes or cell walls of different or same target pathogenic bacteria in different modes in application, and damage the structure and stability of the biocontrol bacteria or inhibit the activity of cell wall degrading enzyme of the biocontrol bacteria, so that the hypha growth, spore germination stopping and dip dyeing capacity of the target pathogenic bacteria are reduced, the combined application achieves the effect of spread spectrum efficient sterilization, and the bacillus thuringiensis is mainly effective on soil insects such as nematodes, overcomes the defects of other two antibacterial strains, and achieves the broad-spectrum target of both pest and disease prevention. In addition, the metabolites of the plant seed and seedling growth regulator also respectively contain special amino acids, plant kinetin, plant growth regulating substances and the like, can effectively promote the growth and development of the protected plants, and synergistically improve the antibacterial and insecticidal application effects of the protected plants, so that the purposes of efficiently controlling the harm and spread of plant seeds and seedlings, promoting the germination of the seeds and the growth of the seedlings, and improving the quality of treated seeds are achieved.
The present invention and its advantageous effects are further illustrated by the following examples and test examples.
1. Examples 1 to 9: the composition ratio of each component of the composite biocontrol strain seed treating agent is shown in table 1.
Table 1 examples 1-9 the proportions of the components of the composite biocontrol seed treating agent
Annotation: 100kg of composite biocontrol strain seed treating agent is prepared in advance.
2. Examples 10 to 14, a method for preparing a composite biocontrol seed treating agent
Example 10, a preparation method of a composite biocontrol strain seed treating agent, taking the formula of example 1 as an example, comprises the following steps: firstly, mixing bacillus subtilis, bacillus cereus and bacillus thuringiensis fermentation concentrated solution with a carrier subjected to superfine grinding, and spray drying at the temperature of below 70 ℃ to obtain a concentrated and solidified compound bacteria fermentation solution. Secondly, respectively superfine grinding all auxiliary materials, fully mixing the concentrated and solidified 80% composite bacteria fermentation liquor with 0.96% of stabilizer and 6.89% of filler which are uniformly mixed for 30min, finally adding 6.65% of film forming agent and 5.0% of wetting dispersant, uniformly mixing for 10min, adding 0.5% of warning color, and uniformly mixing for 30min. Sieving with 1340 mesh sieve, immediately placing at 28deg.C under clean environment above 10 ten thousand grades, and drying for 5 hr to obtain the composite biocontrol bacterium wettable powder seed treating agent with water content less than 5%. Wherein the carrier is peat.
Example 11, a preparation method of a composite biocontrol strain seed treating agent, taking the formula of example 2 as an example, comprises the following steps: firstly, mixing bacillus subtilis, bacillus cereus and bacillus thuringiensis fermentation concentrated solution with a carrier subjected to superfine grinding, and spray drying at the temperature of below 70 ℃ to obtain a concentrated and solidified compound bacteria fermentation solution. Secondly, respectively superfine grinding all auxiliary materials, fully mixing the concentrated and solidified product of 81% composite bacteria fermentation liquor with 1.0% of stabilizer and 5.50% of filler which are uniformly mixed for 25min, finally adding 8.0% of film forming agent and 4.2% of wetting dispersing agent, uniformly mixing for 8min, adding 0.3% of warning color, and uniformly mixing for 40min. Sieving with 1340 mesh sieve, immediately placing at 28deg.C under clean environment above 10 ten thousand grades, and drying for 6 hr to obtain the composite biocontrol bacterium wettable powder seed treating agent with water content less than 5%. Wherein the carrier is a mixture of potassium humate, urea and trehalose (3:0.5:2).
Example 12, a preparation method of a composite biocontrol strain seed treating agent, taking the formula of example 4 as an example, comprises the following steps: firstly, mixing bacillus subtilis, bacillus cereus and bacillus thuringiensis fermentation concentrated solution with a carrier subjected to superfine grinding, and spray drying at the temperature of below 70 ℃ to obtain a concentrated and solidified compound bacteria fermentation solution. Secondly, respectively superfine grinding all auxiliary materials, fully mixing the concentrated and solidified product of 83% composite bacteria fermentation liquor with uniformly mixed 0.01% stabilizing agent and 5.89% filling agent for 28min, finally, adding 7.5% film forming agent and 3.5% wetting dispersing agent, uniformly mixing for 8min, adding 0.1% warning color, and uniformly mixing for 38min. Sieving with 1340 mesh sieve, immediately placing in clean environment with temperature of 30deg.C and more than 10 ten thousand grades, and drying for 48 hr to obtain the composite biocontrol bacterium wettable powder seed treating agent with water content less than 5%. Wherein the carrier is a mixture of sodium glutamate and phosphate (1:2).
Example 13, a preparation method of a composite biocontrol strain seed treating agent, taking the formula of example 8 as an example, comprises the following steps: firstly, mixing bacillus subtilis, bacillus cereus and bacillus thuringiensis fermentation concentrated solution with a carrier subjected to superfine grinding, and spray drying at the temperature of below 70 ℃ to obtain a concentrated and solidified compound bacteria fermentation solution. Secondly, respectively superfine grinding all auxiliary materials, fully mixing the 89% composite bacteria fermentation liquor concentrated condensate with 1.0% stabilizer and 6.80% filler which are uniformly mixed for 30min, finally, adding 1% film forming agent and 2.1% wetting dispersant, uniformly mixing for 5min, adding 0.1% warning color, and uniformly mixing for 40min. Sieving with 1340 mesh sieve, immediately placing in clean environment with temperature of 29 deg.C and over 10 ten thousand grades, and drying for 5 hr to obtain the composite biocontrol bacterium wettable powder seed treating agent with water content less than 5%. Wherein the carrier is a mixture of polyacrylamide, maltodextrin and phosphate (3:2:3).
Example 14, a preparation method of a composite biocontrol strain seed treating agent, taking the formula of example 9 as an example, comprises the following steps: firstly, mixing bacillus subtilis, bacillus cereus and bacillus thuringiensis fermentation concentrated solution with a carrier subjected to superfine grinding, and spray drying at the temperature of below 70 ℃ to obtain a concentrated and solidified compound bacteria fermentation solution. Secondly, respectively superfine grinding all auxiliary materials, fully mixing the 90% composite bacteria fermentation liquor concentrated condensate with 0.08% stabilizer and 5.50% filler which are uniformly mixed for 20min, finally adding 2.2% film forming agent and 2.0% wetting dispersant, uniformly mixing for 10min, adding 0.2% warning color, and uniformly mixing for 40min. Sieving with 1340 mesh sieve, immediately placing in clean environment with temperature of 30deg.C and more than 10 ten thousand grades, and drying for 24 hr to obtain the composite biocontrol bacterium wettable powder seed treating agent with water content less than 5%. Wherein the carrier is a mixture of peat, urea, chitosan and milk powder (6:1:2:3).
3. Application technology of composite biocontrol strain seed treating agent
1. Application targets: is seed of cucumber, melon, tomato, capsicum, etc., tuber seed of potato, etc., and crop seed of corn, etc.
2. The application method comprises the following steps:
diluting at present. The seed coating can be diluted 100-500 times, mechanical coating can be carried out, manual coating can also be carried out, and the drying temperature is controlled below 35 ℃; seed dressing, diluting 300-1000 times, and seed dressing.
4. Test verification of the composite biocontrol strain seed treating agent
Test example 1 influence of the invention on seed germination
Testing seeds to be treated: cucumber, melon, tomato, capsicum, cabbage, corn.
Selecting healthy and full seeds with uniform size, sterilizing with 75% ethanol for 20min, sterilizing with 0.5% sodium hypochlorite for 1min, and washing with sterile water to remove water. Respectively pouring into 100 times diluted composite biocontrol strain seed treating agent (prepared according to example 4) solution, fully and uniformly stirring, taking out, and airing for standby. The weight-to-volume (g/ml) ratio of the seed to the composite biocontrol strain seed treating agent solution is 1:7.
The treated seeds with uniform coating are selected, and each 20 treated seeds are arranged in a group and are placed in a culture dish with the diameter of 9-12 mm, wherein 3 layers of wet filter paper are paved on the culture dish. Healthy full, uniformly sized untreated seeds were used as controls. Each treatment was repeated 5 times. And (5) culturing in the dark at 26+/-1 ℃, and counting the germination rate of all seeds when 50% of the seeds of the control germinate. The bud with white bud color and no rot and deterioration is used as a normal bud. The results are detailed in Table 2.
TABLE 2 Effect of the invention on seed germination
Annotation: the same column of lowercase letters in the table, different numbers for the same species treated and untreated indicate significant differences at the 0.05 level (p.ltoreq.0.05)
Test example 2: the invention affects the emergence rate of seeds and the growth of seedlings (greenhouse pot culture)
Testing seeds to be treated: cucumber, melon, tomato, capsicum, cabbage, corn.
Selecting healthy and full seeds with uniform size, sterilizing with 75% ethanol for 20min, sterilizing with 0.5% sodium hypochlorite for 1min, and washing with sterile water to remove water. Respectively pouring into 200 times diluted composite biocontrol strain seed treating agent (prepared according to example 5) solution, fully and uniformly stirring, taking out, and airing for standby. The weight-to-volume (g/ml) ratio of the seed to the composite biocontrol strain seed treating agent solution is 1:7.
Selecting the uniformly coated seeds, sowing the seeds into 50-hole seedling trays filled with mixed matrixes (coco coir humus soil vermiculite 0.5:3:0.2), treating 2 trays for each variety, and taking healthy and full uncoated seeds with uniform size as respective controls. And (3) carrying out normal management of moisture preservation (65% -70%) and heat preservation (25+/-1 ℃), counting the emergence rate after 10d, and measuring growth indexes such as root length, plant height, fresh weight of each seedling and the like after 32 days of emergence. The results are detailed in Table 3.
TABLE 3 effects of the invention on seed emergence and seedling growth
Annotation: as in table 2.
Test example 3: the invention treats the prevention and control effect of seeds on soil-borne diseases (greenhouse pot culture)
Testing seeds to be treated: cucumber, cabbage, corn.
Selecting healthy and full seeds with uniform size, sterilizing with 75% ethanol for 20min, sterilizing with 0.5% sodium hypochlorite for 1min, and washing with sterile water to remove water. Respectively pouring the above-mentioned materials into 250 times diluted solution of composite biological control strain seed treatment agent (prepared according to example 1), fully uniformly stirring them, taking out, drying and using for stand-by. The weight-to-volume (g/ml) ratio of the seed to the composite biocontrol strain seed treating agent solution is 1:7.
Preparation of pathogenic bacteria spore suspension: and (3) respectively carrying out activation culture on fusarium oxysporum, fusarium putrescicum and rhizoctonia solani by using a PDA culture medium at 26+/-1 ℃ for 10 d-15 d, eluting with a proper amount of sterile water after a large number of spores or sclerotium are generated, and preparing spore suspension with the spore content of more than 108 cfu.ml < -1 > for later use.
Sterilizing the mixed matrix of the coco coir humus soil vermiculite (0.5:3:0.2), then placing the sterilized mixed matrix in 50 plug seedling trays, and respectively inoculating 20 ml pathogenic bacteria spore suspensions into each plug by adopting a perfusion access method and placing the mixed matrix in a moisturizing way for 3 days, wherein each pathogenic bacteria is inoculated into 12 plug seedling trays. Sowing, wherein each treatment of the seeds is carried out by using 2 plug trays, and the non-coated seeds are used as a control. Culturing at 25+ -1deg.C and humidity of about 70% under light cycle of 10 hr/14 hr. And (5) observing and recording the emergence growth condition every day, investigating the number of the disease-causing plants after 30 days of emergence, and counting the disease-causing plant rate and the prevention and control effect. The results are detailed in Table 4.
The calculation formula is as follows: disease rate (%) =number of disease plants/total number of investigation plants×100
Control effect (%) = (control area disease rate-treatment area disease rate)/control area disease rate×100
TABLE 4 control effect of the treatment of seeds on soil-borne diseases according to the invention
Annotation: 1. fusarium oxysporum, fusarium putrescens and Rhizoctonia solani are pathogenic bacteria of plant fusarium wilt, root rot and rhizoctonia solani respectively; 2. as in table 2.
Test example 4: the seed dressing potato seeds have the effect of preventing and controlling plant diseases and insect pests
Healthy and uniform potato stock seeds (Long potato No. 10) with the weight of 9-10 g are selected, washed with sterile water and dried. Pouring the mixture into a diluted solution of 600 times of the composite biocontrol strain seed treating agent (prepared according to the embodiment 9), fully and uniformly stirring, fishing out, and airing for standby. The weight-volume (g/ml) ratio of the potato stock seed to the composite biocontrol strain seed treating agent solution is 1:10. Seeds were treated as positive control in the same way with 16% Xin Liu carbendazim suspension diluted 100-fold and untreated seeds as blank control.
The test consisted of 3 treatments, namely the seed of the invention treatment, a blank control, a positive control. Every 5 replicates treated, 15 experimental cells total, 33m per group area 2 And (3) arranging random granules. Applying a composite organic fertilizer in winter, deep turning, sowing in the next 4 months, and adopting a ridging and film covering planting mode, wherein each ridge is 120cm wide (adding furrows), the height of each ridge is 2cm, the width of each ridge is 70cm, and the plant spacing is 30cm. Cultivation and management are the same as other cultivated lands. Every 5 days, the emergence, plant growth and pest occurrence are observed and recorded, and the disease types, plant disease rate, pest types, pest population density and the like are counted 25 days after emergence. The results are detailed in Table 5.
Calculation formula, disease rate (%) =number of disease plants/total number of investigation plants×100
Control effect (%) = (control area disease rate-treatment area disease rate)/control area disease rate×100
Rate of reduction of insect population (%) = (insect population density in control area-insect population density in treatment area)/insect population density in control area x 100
TABLE 5 prevention and control effects of seed dressing treated Potato seed of the invention on plant diseases and insect pests
Annotation: 1. the potato is continuously planted in the test land for 3 years, the rainfall in the current year is slightly more, and the average temperature is slightly higher; 2. the pest population density is 5 points selected randomly in each district, each point is 1.5 square meters, 20-25cm is dug along the position 5cm away from the center of the plant, the pest number and the species are investigated, the pest number in unit area is calculated, and the pest population densities of the cutworm and the flammulina velutipes are respectively 0.31 per m 2 0.40/m 2 The method comprises the steps of carrying out a first treatment on the surface of the 3. Pathogenic bacteria of the bacterial wilt, soft rot, early blight, fusarium wilt and root rot are respectively rhizoctonia solani, erwinia carotovora pathotype, alternaria solani, fusarium oxysporum and root knot nematode, and the disease plant rates of untreated seeds are respectively 9.13%, 4.89%, 6.58%, 9.74% and 7.28%; fourth, the statistical analysis is as in table 2.
Data analysis: experimental results (tables 2, 3 and 5) of experimental examples 1, 2 and 4 show that the germination rate, emergence rate and seedling growth of seeds such as cucumber, melon, tomato, capsicum, cabbage, corn and potato treated by the composite biocontrol seed treatment agent coating or seed dressing provided by the invention have obvious promotion effect, and have obvious difference (p is less than or equal to 0.05) from untreated controls. Experimental results (tables 4 and 5) of test examples 3 and 4 show that the prevention and treatment effects of cucumber seeds, cabbage seeds, corn seeds and seed-dressing treated potato seeds by the composite biocontrol seed treatment agent provided by the invention are 76% -84%, the incidence rate of the composite biocontrol seed treatment agent is much lower than that of untreated seeds, and the obvious difference (p is less than or equal to 0.05) exists. The results of example 4 (Table 5) further show that the composite biocontrol seed treatment agent provided by the invention has the control effects of 61.44% and 70.60% on potato seeds treated with the seed dressing agent, respectively, the control effects of 16% Xin Liu carbendazim suspension agent without the control agent are strong (p is less than or equal to 0.05), the control effects of 77.29%, 78.63% and 79.19% on disease damping-off, soft rot and fusarium wilt respectively, the control effects of 16% Xin Liu carbendazim suspension agent without the control agent are not significant (p is more than or equal to 0.05), the control effects of 76.42% and 77.53% on early blight and root rot of potato respectively, and the control effects of 16% Xin Liu carbendazim suspension agent without the control agent are strong (p is less than or equal to 0.05).
The preservation number mentioned in the invention is CGMCC No.24484, CGMCC No.24485,
the biological material samples of CGMCC No. 24483' are all applied for an invention patent in 2022, 10 and 26 days and published before the application date of the invention, and the application numbers are respectively as follows: 2022110334179, 2022110336511, 2022110345188.
Claims (8)
1. The composite biocontrol strain seed treating agent is characterized by being prepared from the following components in percentage by weight: 80 to 90 percent of effective active components, 1 to 8.0 percent of film forming agent, 2.0 to 5.0 percent of wetting dispersant, 0.01 to 1.0 percent of stabilizer, 5.50 to 6.89 percent of filling agent and 0.1 to 0.5 percent of warning color; the effective active component is concentrated and solidified matters of the equal proportion composite bacterial fermentation liquid of bacillus subtilis and bacillus cereus; the bacillus subtilis is bacillus subtilis Bacillus subtilis, and the preservation number is CGMCC No.24484; the Bacillus cereus is Bacillus cereus with a preservation number of CGMCC No.24485; the bacillus thuringiensis is bacillus thuringiensis Bacillus thuringiensis and has a preservation number of CGMCC No.24483.
2. The composite biocontrol strain seed treating agent according to claim 1, wherein: the film forming agent is one or a mixture of several of sodium carboxymethyl starch, soluble starch, phosphorylated starch-oxidized starch acetate, sodium carboxymethyl cellulose, hydroxypropyl methyl cellulose, sodium alginate, PVA17-88, PVA05-88, polyvinyl acetate, polyacrylamide, polyvinylpyrrolidone, polyacrylate, acacia, tragacanth, oxidized hydroxypropyl starch and calcium silicate in any ratio.
3. The composite biocontrol strain seed treating agent according to claim 1, wherein: the wetting dispersant is one or a mixture of several of triethanolamine oleate, morwetEFW, ethylan1008W, ethylanNS-500LQ, dodecyl sulfonate, dioctyl sodium succinate sulfonate, span80, tween-80, morwet D-425, morwet D-500, lignin sulfonate, nekal BX, dispersinf AgentNNO, alkyl succinate sulfonate, dibutyl naphthalene sulfonate and the like.
4. The composite biocontrol strain seed treating agent of claim 1, wherein the stabilizer is one or a mixture of any ratio of magnesium aluminum silicate, aluminum stearate, hydrogen citrate, tartrate, magnesium succinate, sodium glycinate, organosilicon and fatty alcohol polyglycol ether.
5. The composite biocontrol strain seed treating agent of claim 1, wherein the filler is one or a mixture of several of bentonite, diatomite, attapulgite, calcium carbonate, dextrin, kaolin, white carbon black, agar, monopotassium phosphate, calcium superphosphate, ferrous sulfate, trace elements and the like in any ratio.
6. The composite biocontrol strain seed treating agent of claim 1, wherein the warning color is one of weak acid dyes such as black T, complex yellow GR, rose essence and the like.
7. The method for preparing the composite biocontrol strain seed treating agent according to claim 1, which is characterized by comprising the following steps: the method comprises the following specific steps: firstly, mixing the Bacillus subtilis Bacillus subtilis with the preservation number of CGMCC No.24484 and Bacillus cereus with the preservation numbers of CGMCC No.24485 and Bacillus thuringiensis Bacillus thuringiensis and the fermentation concentrated solution with the preservation number of CGMCC No.24483 with a superfine crushed carrier, and spray-drying at the temperature below 70 ℃ to obtain a concentrated condensate of the composite bacterial fermentation solution; secondly, respectively superfine grinding all auxiliary materials, and fully and uniformly mixing 80% -90% of concentrated and solidified compound bacteria fermentation liquor, 0.01% -1.0% of stabilizer and 5.50% -6.89% of filler for 20 min-30 min; finally, adding 1.0 to 8.0 percent of film forming agent and 2.0 to 5.0 percent of wetting dispersant, uniformly mixing for 5 to 0min, adding 0.1 to 0.5 percent of warning color, and uniformly mixing for 30 to 40min; sieving with 1340 mesh sieve, immediately placing in clean environment with temperature of 28-30deg.C and more than 10 ten thousand grades, drying for 5-48 h to obtain composite biological control bacteria solid seed treating agent with water content less than 5%; wherein the carrier is one or more of potassium humate, peat, alginate, urea, polyacrylamide, trehalose, chitosan, sodium glutamate, maltodextrin, milk powder, phosphate and the like.
8. The use of a composite biocontrol seed treatment agent according to any one of claims 1-7, wherein: the method is used for preventing and controlling plant diseases and insect pests in seeds of vegetables and crop seeds and in seedling stage; the composite biocontrol strain seed treating agent is coated or mixed with seeds, and is diluted in the prior art; the seed coating can be diluted 100-500 times, and can be mechanically coated or manually coated, and the drying temperature is controlled below 35 ℃; seed dressing, diluting 300-1000 times, and seed dressing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310503745.9A CN116548472A (en) | 2023-05-06 | 2023-05-06 | Composite biocontrol strain seed treating agent, preparation method and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310503745.9A CN116548472A (en) | 2023-05-06 | 2023-05-06 | Composite biocontrol strain seed treating agent, preparation method and application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN116548472A true CN116548472A (en) | 2023-08-08 |
Family
ID=87501189
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310503745.9A Pending CN116548472A (en) | 2023-05-06 | 2023-05-06 | Composite biocontrol strain seed treating agent, preparation method and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN116548472A (en) |
-
2023
- 2023-05-06 CN CN202310503745.9A patent/CN116548472A/en active Pending
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP4810151B2 (en) | Control agent for diseases occurring during the seedling raising season of rice | |
| KR101441614B1 (en) | Insecticide or compost composition comprising the leaves of Ginkgo biloba by water aging | |
| CN107667737A (en) | A kind of method of no agricultural chemicals stereo synthesis prevention and control fruits and vegetables pest and disease damage | |
| CN105557689A (en) | Bacillus wettable powder containing brassinolide and preparation method thereof | |
| CN105594698B (en) | A kind of preparation method of bacillus laterosporus wettable powder | |
| CN105557757A (en) | Preparation method for bacillus methylotrophicus wettable powder | |
| Watanabe et al. | Effects of soil pH on rhizoctonia damping-off of sugar beet and disease suppression induced by soil amendment with crop residues | |
| CN116918832A (en) | Composite biocontrol microbial agent, preparation method, application and application method | |
| CN107372615A (en) | A kind of seed dressing and its preparation method and application | |
| US5194258A (en) | Production of enhanced biocontrol agents | |
| Sutthisa et al. | Development of Trichoderma formulation and application to control durian anthracnose disease | |
| Apazhev et al. | Ensuring the environmental safety of food when using biological products in the protection of cabbage agrocenosis | |
| Biswas et al. | Organic farming to mitigate biotic stresses under climate change scenario | |
| CN116548472A (en) | Composite biocontrol strain seed treating agent, preparation method and application | |
| CN115161214A (en) | Biological organic fertilizer for preventing and controlling soil insects, preparation method and application | |
| KR100637329B1 (en) | Microbial pesticides having fungicidal and insecticidal activity | |
| EP0544039B1 (en) | Production of enhanced biocontrol agents | |
| CN110612988A (en) | Application of Arthrobacter ureafaciens in prevention and treatment of corn diseases | |
| Utkhede et al. | Development of biological control of apple replant disease | |
| Biswas et al. | Use of Amritpani: An Excellent Bio-Enhancer for Sustainable Agriculture: An Overview | |
| RU2807997C1 (en) | Method of cultivation of agricultural crops | |
| Al-Karaawi et al. | Integrated Control of White Rot in Eggplant by using Pseudomonas flourescens, Penicillium Commune and Trichoderma asperellum in Iraq | |
| CN116762826A (en) | Plant pathogenic soil biological control agent, preparation method, application and application method | |
| Harman et al. | Potential and existing uses of | |
| Poromarto et al. | Effectiveness of fermentation extract of cabbage leaves from harvesting waste to control purple blotch of garlic |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |