CN116532186A - Preparation device and preparation method of pathogen detection object - Google Patents
Preparation device and preparation method of pathogen detection object Download PDFInfo
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- CN116532186A CN116532186A CN202310822632.5A CN202310822632A CN116532186A CN 116532186 A CN116532186 A CN 116532186A CN 202310822632 A CN202310822632 A CN 202310822632A CN 116532186 A CN116532186 A CN 116532186A
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- 238000002360 preparation method Methods 0.000 title claims abstract description 114
- 238000001514 detection method Methods 0.000 title claims abstract description 38
- 244000052769 pathogen Species 0.000 title claims abstract description 25
- 230000001717 pathogenic effect Effects 0.000 title claims abstract description 25
- 238000012545 processing Methods 0.000 claims description 45
- 230000009471 action Effects 0.000 claims description 40
- 230000001105 regulatory effect Effects 0.000 claims description 26
- 238000000034 method Methods 0.000 claims description 24
- 230000033001 locomotion Effects 0.000 claims description 18
- 230000008569 process Effects 0.000 claims description 18
- 230000007246 mechanism Effects 0.000 claims description 12
- 230000005540 biological transmission Effects 0.000 claims description 10
- 230000001276 controlling effect Effects 0.000 claims description 10
- 238000007599 discharging Methods 0.000 claims description 10
- 230000001681 protective effect Effects 0.000 claims description 5
- 230000001174 ascending effect Effects 0.000 claims description 4
- 238000012958 reprocessing Methods 0.000 claims description 4
- 230000005389 magnetism Effects 0.000 claims description 2
- 210000001519 tissue Anatomy 0.000 description 23
- 108090000623 proteins and genes Proteins 0.000 description 6
- 210000004027 cell Anatomy 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 239000012634 fragment Substances 0.000 description 4
- 238000005192 partition Methods 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 3
- 208000003322 Coinfection Diseases 0.000 description 2
- 230000026058 directional locomotion Effects 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000000630 rising effect Effects 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000002200 mouth mucosa Anatomy 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000001360 synchronised effect Effects 0.000 description 1
- 210000004881 tumor cell Anatomy 0.000 description 1
Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B02—CRUSHING, PULVERISING, OR DISINTEGRATING; PREPARATORY TREATMENT OF GRAIN FOR MILLING
- B02C—CRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL; MILLING GRAIN
- B02C4/00—Crushing or disintegrating by roller mills
- B02C4/02—Crushing or disintegrating by roller mills with two or more rollers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B02—CRUSHING, PULVERISING, OR DISINTEGRATING; PREPARATORY TREATMENT OF GRAIN FOR MILLING
- B02C—CRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL; MILLING GRAIN
- B02C23/00—Auxiliary methods or auxiliary devices or accessories specially adapted for crushing or disintegrating not provided for in preceding groups or not specially adapted to apparatus covered by a single preceding group
- B02C23/08—Separating or sorting of material, associated with crushing or disintegrating
- B02C23/10—Separating or sorting of material, associated with crushing or disintegrating with separator arranged in discharge path of crushing or disintegrating zone
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B02—CRUSHING, PULVERISING, OR DISINTEGRATING; PREPARATORY TREATMENT OF GRAIN FOR MILLING
- B02C—CRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL; MILLING GRAIN
- B02C4/00—Crushing or disintegrating by roller mills
- B02C4/28—Details
- B02C4/42—Driving mechanisms; Roller speed control
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
- G01N2001/2866—Grinding or homogeneising
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a preparation device and a preparation method of a pathogen detection object, and relates to the technical field of preparation instruments of pathogen detection objects.
Description
Technical Field
The invention relates to the technical field of pathogen detection object preparation instruments, in particular to a preparation device and a preparation method of a pathogen detection object.
Background
The gene detection is a technology for detecting DNA through blood, other body fluids or tissue cells, namely taking oral mucosa cells or other tissue cells which fall off by a detected person, amplifying the gene information, detecting DNA molecular information in the cells of the detected person through specific equipment, analyzing various gene conditions contained in the DNA molecular information, and enabling people to know own gene information, so that the risk of a certain disease of the body is predicted or the cause of the disease is determined;
the gene detection generally comprises three steps of extraction, amplification and detection, wherein when each type of tissue sample of an animal body is taken as a detection object to be detected, the tissue sample is firstly required to be prepared into a plurality of detection objects, namely, the tissue sample is taken out of the animal body, the tissue sample is crushed into detection object fragments with different sizes through special equipment, the DNA detection equipment is utilized to detect the plurality of detection object fragments, and the pathogen condition of the diseased animal can be comprehensively known, whether the pathogen is infected by viruses, bacteria or mixed infection or secondary infection, even the pathogen is identified by typing and the like; however, in the process of preparing the actual detection object fragments, the detection personnel usually uses a crushing tool to crush the tissue sample, and the required detection object fragments are generally about 15 blocks during detection, because the accuracy of the crushing size is related to the operation proficiency of the detection personnel, and the volume of the tissue sample to be taken out during detection is within a certain range, in the process of crushing, the condition that part of the detection object is too large or too small in size often occurs due to misoperation, and the influence on the subsequent gene detection result is easy to occur, therefore, the preparation device and the preparation method of the pathogen detection object are provided.
Disclosure of Invention
The invention aims to provide a preparation device and a preparation method of a pathogen detection object, which are used for solving the problems in the background technology.
In order to achieve the above purpose, the present invention provides the following technical solutions: the preparation device of the pathogen detection object comprises a preparation frame, a preparation tank body arranged below the preparation frame, wherein a first treatment plate which is in sliding connection with the inner wall of the preparation frame is arranged in the preparation tank body, a second treatment plate and a third treatment plate are arranged in the preparation tank body, the third treatment plate is arranged below the second treatment plate, crushing rollers are symmetrically arranged in the preparation frame, two crushing rollers are arranged above the first treatment plate, a connecting piece which is used for connecting the crushing rollers is arranged in the preparation frame, and a driving piece which is arranged in the preparation frame is used for driving the crushing rollers to perform position adjustment through the connecting piece, an action piece which is used for controlling the crushing rollers to perform crushing work is also arranged in the preparation frame, and the first treatment plate conveys tissue samples crushed into various sizes to the second treatment plate in a falling mode for reprocessing; the preparation jar is internal to be provided with the axis body, just parallel arrangement has two regulation and control boards on the axis body, two the regulation and control board is used for carrying out reprocessing work to processing board two and processing board three, be provided with the pan feeding frame on the preparation frame, and parallel arrangement has the play work frame on the preparation jar body.
Preferably, the connecting piece is including setting up in the inside carriage of preparation frame, just the carriage both sides are provided with the spacing slide rail that is used for carrying out sliding connection with between the broken cylinder, and two still be connected with the connection grillage between spacing slide rail and the carriage, two be provided with the spacing grillage on the broken cylinder, just rotate between broken cylinder and the spacing grillage and be connected, the carriage is inside to be provided with the spout that carries out sliding connection with between the spacing grillage, wherein preparation frame internally mounted has the baffle, and is located the carriage top, still install on the carriage and extend the cylinder, just extend and carry out sliding connection between cylinder and the baffle inner wall.
Preferably, the driving piece is including setting up in the inside lead screw of preparation frame, just the lead screw both ends all run through preparation frame inner wall and extend to outside, install the drive framework on the lead screw, just extend cylinder tip and be located the drive framework is inside, and the inside symmetry of preparation frame installs a plurality of guide posts that carry out sliding connection with the drive framework between, drive framework both sides inner wall is provided with the regulation and control groove.
Preferably, sliding columns which are in sliding connection with the regulating and controlling grooves are symmetrically arranged on the extending columns, and the regulating and controlling grooves are composed of a descending area, a straight running area, an ascending area and a limiting area.
Preferably, the preparation frame is externally and symmetrically provided with a protection shell, one of the protection shells is provided with a first servo motor, an output end of the first servo motor is connected with one end of the screw rod, two sides of the preparation frame are provided with rotating cylinders which are rotationally connected with the preparation frame, the two sides of the preparation frame are respectively provided with a transmission mechanism, two ends of each transmission mechanism are respectively arranged on the screw rod and the rotating cylinders, and the end parts of the rotating cylinders penetrate through the outer wall of the preparation frame and extend to the inside of the preparation frame.
Preferably, the action piece is including setting up in the inside circular carousel of preparation frame, just circular carousel is connected with the cylinder tip that rotates, just install a plurality of magnet one and magnet two on the circular carousel, just magnet one is in opposite state with the magnetism of magnet two, wherein angle between magnet one and the magnet two is 90, two all install the magnet piece on the limiting plate frame, the magnet one produces the repulsion effort to the magnet piece, just magnet two produce the attraction effort to the magnet piece.
Preferably, the rotating cylinder is further provided with an intermittent gear, and a rotating shaft which is rotationally connected with the inner wall of the protecting shell is arranged in the two protecting shells, wherein the rotating shaft is further provided with a gear body, the gear body is positioned on the meshing track of the intermittent gear, and the rotating shaft is further provided with an action rod.
Preferably, the processing plate is provided with a stress rod piece on one side and two sides, the stress rod piece is located on the motion track of the action rod, cavities are formed in two sides of the preparation frame, reset springs are mounted on inner walls of two ends of the cavities, and the ends of the reset springs are connected with two ends of the stress rod piece.
Preferably, the second servo motor is installed on the preparation tank body, the output end of the second servo motor penetrates through the inner wall of the preparation tank body and is connected with the shaft body, the second bottom of the processing plate and the third top of the processing plate are symmetrically provided with stressed roller pieces, the stressed roller pieces are located on the motion tracks of the two regulating plates, the second processing plate and the third processing plate are rotationally connected with the inner wall of the preparation tank body, a torsion spring is further connected between the second processing plate and the third processing plate and the inner wall of the preparation tank body, and the second processing plate and the third processing plate are provided with discharge pipe orifices.
A method for preparing a pathogen detection object, comprising the steps of:
s1, feeding a feeding frame, starting a first servo motor and a second servo motor, and rotating a screw rod to enable a driving frame body to be subjected to position adjustment under the action of a guide post, wherein a sliding column body on an extending column body performs limiting movement on a regulating groove, namely the extending column body performs descending movement, and the extending column body drives a supporting plate frame, a limiting sliding rail, a connecting plate frame, a crushing roller and a limiting plate frame to perform descending movement;
s2, the screw rod drives the rotary column to synchronously rotate under the action of the transmission mechanism, the rotary column drives the magnet I and the magnet II to act through the circular turntable, and the magnet blocks on the limiting plate frame respectively reciprocate under the action of the magnet I and the magnet II in the rotation process of the circular turntable, so that the tissue samples are crushed and prepared;
s3, the extending column body drives the supporting plate frame, the limiting sliding rail, the connecting plate frame, the crushing roller and the limiting plate frame to ascend, the acting rod acts on the stressed rod piece under the meshing action of the intermittent gear and the gear body, the stressed rod piece drives the first treatment plate to adjust the position, and in the adjustment process, acting force is applied to the two reset springs, namely the first treatment plate is rocked;
and S4, when the servo motor II is started, the output end of the servo motor II drives the shaft body to rotate, the regulating and controlling plate on the shaft body acts on the stressed roller piece, namely the processing plate II and the processing plate III perform shaking action, namely objects to be detected with different sizes are screened out, and the prepared objects to be detected are discharged by utilizing the two discharging frames, namely the preparation is completed.
Compared with the prior art, the invention has the beneficial effects that:
the invention utilizes the crushing roller and the first treatment plate to crush and prepare the tissue samples, utilizes the second treatment plate and the third treatment plate to classify and prepare the tissue samples with different sizes, can realize the position adjustment of the extension column under the action of the driving frame body and the regulating groove, and utilizes the supporting plate frame, the connecting plate frame, the limiting slide rail and the limiting plate frame to drive the crushing roller to synchronously adjust the position, can control the acting rod to apply acting force to the stressed rod piece under the action of the intermittent gear and the gear body, and can control the first treatment plate to shake under the elastic action of the reset spring so as to ensure that the crushed tumor cells are classified.
The invention can effectively control the angle adjustment of the second processing plate and the third processing plate by utilizing the position relation between the adjusting and controlling plate and the stress roller piece, and can output and store the object to be measured under the action of the discharging pipe orifice and the discharging frame, thereby realizing the purpose of automatically preparing the object to be measured.
Drawings
FIG. 1 is a schematic diagram of the overall structure of the present invention;
FIG. 2 is a schematic diagram of a preparation frame structure of the present invention;
FIG. 3 is a schematic illustration showing the separation of a partial structure of a manufacturing frame and a structure of a connecting piece according to the present invention;
FIG. 4 is a schematic view of the connector structure of the present invention;
FIG. 5 is a schematic view of a part of the structure of the driving member according to the present invention;
FIG. 6 is a schematic diagram of a control slot structure according to the present invention;
FIG. 7 is a schematic view of the external partial structure of the preparation frame of the present invention;
FIG. 8 is a schematic view of a partial structure of the present invention;
FIG. 9 is a schematic view of a connection member of a process plate according to the present invention;
FIG. 10 is a schematic diagram of the structure of a manufacturing tank according to the present invention;
FIG. 11 is a schematic view of the internal structure of the preparation tank of the present invention;
FIG. 12 is a schematic view of a second embodiment of a processing plate of the present invention;
FIG. 13 is a schematic view of a shaft and a regulating plate structure according to the present invention.
In the figure: 1. preparing a frame; 2. preparing a tank body; 3. a first treatment plate; 4. a second treatment plate; 5. a third treatment plate; 6. a crushing roller; 7. a connecting piece; 71. a support plate frame; 72. a limit sliding rail; 73. a connecting plate frame; 74. limiting plate frame; 75. a chute; 76. an extension column; 77. a skidding column; 78. a magnet block; 8. a driving member; 81. a screw rod; 82. a drive frame; 83. a guide post; 85. a regulating groove; 86. a descent area; 87. a straight region; 88. a rising region; 89. a limit area; 9. an action member; 91. a circular turntable; 92. a first magnet; 93. a second magnet; 94. intermittent gears; 95. a rotating shaft; 96. a gear body; 97. an action lever; 98. a force-bearing rod piece; 99. a cavity; 90. a return spring; 10. a shaft body; 11. a regulation plate; 12. a feeding frame; 13. a discharging frame; 14. a protective housing; 141. a servo motor I; 142. rotating the column; 143. a transmission mechanism; 15. a servo motor II; 151. a force-bearing roller member; 152. a torsion spring; 153. and a discharging pipe orifice.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Referring to fig. 1-13, the present invention provides a technical solution: the invention relates to a preparation device of a pathogen detection object, which aims at improving the technical problems in the background technology, and comprises a preparation frame 1, a preparation tank body 2 fixedly arranged below the preparation frame 1, wherein a treatment plate I3 which is in sliding connection with the inner wall of the preparation frame 1 is slidably arranged in the preparation frame 1, further description is provided that the treatment plate I3 is used for placing a tissue sample, a feeding frame 12 is arranged on the preparation frame 1, namely, the extracted tissue sample can be conveyed to the treatment plate I3 through the feeding frame 12, and a treatment plate II 4 and a treatment plate III 5 are rotatably arranged in the preparation tank body 2, and the treatment plate I3 and the treatment plate II 4 are respectively provided with a plurality of through holes, and the aperture relation of the through holes of the two is as follows: the aperture on the first treatment plate 3 is larger than the aperture on the second treatment plate 4, wherein the third treatment plate 5 is positioned below the second treatment plate 4, crushing rollers 6 are symmetrically arranged inside the preparation frame 1, the crushing rollers 6 are used for crushing tissue samples, two crushing rollers 6 are positioned above the first treatment plate 3, a connecting piece 7 used for connecting the crushing rollers 6 is arranged inside the preparation frame 1, and a driving piece 8 is arranged inside the preparation frame 1, wherein the driving piece 8 is used for driving the crushing rollers 6 to carry out position adjustment through the connecting piece 7;
as a further limitation of the present invention, the connecting piece 7 comprises a support plate frame 71 arranged inside the preparation frame 1, wherein an extension column body 76 is installed on the support plate frame 71, further describing that a partition plate is fixedly installed inside the preparation frame 1, the end part of the extension column body 76 penetrates through the inner wall of the partition plate and extends to the upper part of the partition plate (the support plate frame 71 is positioned below the partition plate), wherein sliding columns 77 are symmetrically and fixedly installed on the extension column body 76, limiting slide rails 72 for sliding connection with the crushing roller 6 are fixedly installed on two sides of the support plate frame 71, a connecting plate frame 73 is fixedly connected between the two limiting slide rails 72 and the support plate frame 71, a limiting plate frame 74 is rotatably installed on each of the two crushing rollers 6, the crushing roller 6 is rotatably connected with the limiting plate frame 74, and sliding grooves 75 for sliding connection with the limiting plate frame 74 are arranged inside the support plate frame 71; namely, the two crushing rollers 6 can move on the limiting slide rail 72, and meanwhile, the crushing rollers 6 can also rotate on the limiting plate frame 74 (the limiting plate frame 74 can adjust the position inside the slide groove 75), wherein the two limiting plate frames 74 are fixedly provided with the magnet blocks 78;
as a further limitation of the present invention, the driving member 8 includes a screw rod 81 installed inside the preparation frame 1, and both ends of the screw rod 81 penetrate through the inner wall of the preparation frame 1 and extend to the outside, wherein a protective housing 14 is symmetrically and fixedly installed outside the preparation frame 1, a first servo motor 141 is installed on one protective housing 14, an output end of the first servo motor 141 is connected with one end of the screw rod 81, a driving frame 82 is installed on the screw rod 81 (the driving frame 82 is located inside the preparation frame 1), an end of the extension column 76 is located inside the driving frame 82, and a plurality of guide posts 83 slidably connected with the driving frame 82 are symmetrically installed inside the preparation frame 1, and regulating grooves 85 slidably connected with the sliding columns 77 are provided on both inner walls of the two sides of the driving frame 82, that is, the regulating grooves 85 in the present invention are used for limiting the movement of the sliding columns 77, wherein the regulating grooves 85 are composed of a descending area 86, a straight running area 87, a rising area 88 and a limiting area 89; further, two sides of the preparation frame 1 are rotatably provided with rotating columns 142 which are rotatably connected with the preparation frame 1, the interiors of the two protection shells 14 are respectively provided with a transmission mechanism 143, and two ends of each transmission mechanism 143 are respectively arranged on the screw rod 81 and the rotating columns 142, wherein the end parts of the rotating columns 142 penetrate through the outer wall of the preparation frame 1 and extend into the interior of the preparation frame, an acting piece 9 for controlling the crushing roller 6 to perform crushing work is also arranged in the preparation frame 1, and a first processing plate 3 conveys a tissue sample crushed into a plurality of sizes to a second processing plate 4 in a falling mode for reprocessing;
as a further limitation of the present invention, the acting element 9 includes a circular turntable 91 disposed inside the preparation frame 1, the circular turntable 91 is fixedly connected with the end of the rotating column 142, a plurality of first magnets 92 and second magnets 93 are mounted on the circular turntable 91, the first magnets 92 and the second magnets 93 are in opposite magnetic states, and the plurality of first magnets 92 and the second magnets 93 are disposed adjacently on the circular turntable 91, wherein an angle between the first magnets 92 and the second magnets 93 is 90 °, the first magnets 92 generate a repulsive force to the magnet block 78, and the second magnets 93 generate a attractive force to the magnet block 78, further describing that when the crushing drum 6 is lowered to the surface of the first processing plate 3 and performs the crushing operation, the magnet block 78 on the limiting plate frame 74 corresponds to a magnet (the first magnet 92 or the second magnet 93) at the lowest point of the circular turntable 91, and when the first magnet 92 rotates with the circular turntable 91 and is at the lowest point, the first magnets 92 generate a repulsive force to the magnet block 78, and when the second magnet 93 rotates with the circular turntable 91 is at the lowest point, the second magnets 93 generate a repulsive force to the magnet block 78; the rotating column 142 is fixedly provided with an intermittent gear 94, the two protecting housings 14 are internally provided with rotating shafts 95 which are rotationally connected with the inner walls of the protecting housings, the rotating shafts 95 are fixedly provided with gear bodies 96, the gear bodies 96 are positioned on meshing tracks of the intermittent gear 94, the rotating shafts 95 are further provided with action bars 97, the two sides of the first treatment plate 3 are fixedly provided with stress bars 98, the stress bars 98 are positioned on moving tracks of the action bars 97, two sides of the preparation frame 1 are provided with cavities 99, the inner walls of two ends of the cavities 99 are fixedly provided with reset springs 90, and the ends of the reset springs 90 are connected with the two ends of the stress bars 98.
Specifically, the tissue sample enters the first treatment plate 3 from the feeding frame 12, the first servo motor 141 is started, the output end of the first servo motor drives the screw rod 81 to rotate, the driving frame 82 on the screw rod 81 performs directional motion under the action of the guide post 83, the regulating groove 85 on the driving frame 82 acts on the sliding column 77 in the moving process, the sliding column 77 is driven to perform position adjustment after acting force, the sliding column 77 is positioned at the tail end of the descending region 86 of the regulating groove 85 in the initial state, the intermittent gear 94 is not meshed with the gear body 96 at this time, the inner wall of the descending region 86 acts on the sliding column 77 in the moving process of the driving frame 82, the sliding column 77 enables the extending column 76 to perform descending motion, and the extending column 76 drives the connecting plate frame 73, the limiting slide rail 72, the limiting plate frame 74 and the crushing roller 6 to perform synchronous descending motion through the supporting plate frame 71, when the sliding column 77 moves to the end of the straight-going area 87, the crushing roller 6 contacts with the first processing plate 3, the first servo motor 141 continues to rotate, the driving frame 82 continues to move, the sliding column 77 moves from the end of the straight-going area 87 to the initial end, the first servo motor 141 rotates the rotating column 142 under the action of the transmission mechanism 143 during rotation, it should be noted that the transmission mechanism 143 in the invention is a belt pulley, the rotating column 142 rotates the circular turntable 91 in the preparation frame 1, the first magnet 92 and the second magnet 93 on the circular turntable 91 synchronously rotate along with the circular turntable, so that when the crushing roller 6 is positioned on the first processing plate 3, if the first magnet 92 moves to the lowest point along with the circular turntable 91, the first magnet 92 generates repulsive force to the magnet block 78, the magnet block 78 is acted to force the limiting plate frame 74 to drive the crushing roller 6 to perform directional motion, during the motion process, namely, the tissue sample is crushed, when the magnet II 93 rotates along with the circular turntable 91 and is located at the lowest point, the magnet II 93 generates attractive force to the magnet block 78, at this moment, the magnet block 78 is acted to force the limiting plate frame 74 to drive the crushing roller 6 to perform reverse motion, namely, the tissue sample is crushed, further, when the sliding column 77 is located in the descending region 86 and the straight region 87, the intermittent gear 94 is not meshed with the gear body 96, when the sliding column 77 moves to the ascending region 88, at this moment, the crushing roller 6 (and a connecting part thereon) is lifted, namely, is not contacted with the first processing plate 3 any more, at this moment, the intermittent gear 94 is meshed with the gear body 96, namely, the gear body 96 is rotated by the meshing force, so as to drive the rotating shaft 95 to synchronously rotate, the action rod 97 on the rotating shaft 95 acts on the force-bearing rod 98 in the rotating process, the force-bearing rod 98 compresses one of the return springs 90 by the acting force, the other return spring 90 stretches, the force-bearing rod 98 moves and drives the first treatment plate 3 to shake, so that the crushed tissue sample on the first treatment plate 3 falls onto the second treatment plate 4 through the through hole on the first treatment plate 3, further, when the sliding column 77 ascends in the ascending area 88 and the limiting area 89, the intermittent gear 94 is meshed with the gear body 96, and the gear body 96 and the rotating shaft 95 rotate for one circle, namely, under the action of the action rod 97, the force-bearing rod 98 and the return spring 90, the crushed tissue sample can be treated by the first treatment plate 3.
Furthermore, a second servo motor 15 is fixedly installed on the preparation tank 2, the output end of the second servo motor 15 penetrates through the inner wall of the preparation tank 2 and is fixedly connected with the shaft 10, the bottom of the second treatment plate 4 and the top of the third treatment plate 5 are symmetrically provided with stress roller pieces 151, the two regulation plates 11 are composed of raised areas and recessed areas, an angle difference exists between the two regulation plates 11, the stress roller pieces 151 are located on the motion track of the two regulation plates 11, the second treatment plate 4 and the third treatment plate 5 are in rotary connection with the inner wall of the preparation tank 2, as shown in fig. 11-13, a torsion spring 152 is further connected between the second treatment plate 4 and the third treatment plate 5 and the inner wall of the preparation tank 2, and the second treatment plate 4 and the third treatment plate 5 are respectively provided with a discharge pipe orifice 153, further illustrating that the discharge pipe orifice 153 is provided with a valve, and the discharge frame 13 can be pulled out from the preparation tank 2;
specifically, the second servo motor 15 is started, the output end of the second servo motor drives the shaft body 10 to rotate, the regulating plate 11 on the shaft body 10 synchronously rotates along with the second servo motor, in the rotation process of the regulating plate 11, the protruding area on the regulating plate acts on the stressed roller piece 151 at the bottom of the second processing plate 4, the recessed area of the regulating plate is located above the stressed roller piece 151 at the top of the third processing plate 5, namely, the stressed roller piece 151 at the bottom of the second processing plate 4 receives the acting force of the protruding area, in the preparation tank 2 rotates, in the rotation process, the torsion spring 152 is in a torsion state, the second processing plate 4 shakes, when the recessed area on the regulating plate 11 is located below the stressed roller piece 151 of the second processing plate 4, the stressed roller piece 151 at the top of the third processing plate 5 receives the acting force of the protruding area on the regulating plate 11, the third processing plate 5 performs angle adjustment, namely, plays a shaking role, in the shaking process, the discharging pipe orifice 153 on the third processing plate 5 is in an opened state, broken tissue samples in the inside the discharging frame 13 is located in the discharging pipe orifice 153 on the second processing plate 4 is in a closed state, after the preparation is completed, and the material is taken out (when the second processing plate 4 is in the inclined state acts on the stressed roller piece 151).
A method for preparing a pathogen detection object, comprising the steps of:
s1, feeding the feeding frame 12, starting a first servo motor 141 and a second servo motor 15, rotating a screw rod 81 to enable a driving frame 82 to be subjected to position adjustment under the action of a guide post 83, and limiting a sliding post 77 on an extending post 76 on a regulating groove 85, namely, enabling the extending post 76 to perform descending motion, wherein the extending post 76 drives a supporting plate frame 71, a limiting slide rail 72, a connecting plate frame 73, a crushing roller 6 and a limiting plate frame 74 to perform descending motion;
s2, the screw rod 81 drives the rotary column 142 to synchronously rotate under the action of the transmission mechanism 143, the rotary column 142 drives the first magnet 92 and the second magnet 93 to act through the circular turntable 91, and the magnet blocks 78 on the limiting plate frame 74 respectively reciprocate under the action of the first magnet 92 and the second magnet 93 in the rotating process of the circular turntable 91, so that the tissue sample is crushed and prepared;
s3, the extending column body 76 drives the supporting plate frame 71, the limiting slide rail 72, the connecting plate frame 73, the crushing roller 6 and the limiting plate frame 74 to ascend, under the meshing action of the intermittent gear 94 and the gear body 96, the action rod 97 acts on the force-bearing rod piece 98, the force-bearing rod piece 98 drives the first treatment plate 3 to adjust the position, and in the adjustment process, acting force is applied to the two reset springs 90, namely the first treatment plate 3 shakes;
and S4, when the second servo motor 15 is started, the output end of the second servo motor drives the shaft body 10 to rotate, the regulating and controlling plate 11 on the shaft body 10 acts on the stressed roller piece 151, namely the second processing plate 4 and the third processing plate 5 perform shaking motion, namely objects to be detected with different sizes are screened out, and the prepared objects to be detected are discharged by utilizing the two discharging frames 13, so that the preparation is completed.
It is noted that relational terms such as first and second, and the like are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Moreover, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (10)
1. A preparation facilities of pathogen detection thing, characterized in that: the preparation method comprises a preparation frame (1), a preparation tank body (2) arranged below the preparation frame (1), wherein a first treatment plate (3) which is connected with the inner wall of the preparation frame (1) in a sliding way is arranged inside the preparation tank body (2), a second treatment plate (4) and a third treatment plate (5) are arranged inside the preparation tank body (2), the third treatment plate (5) is positioned below the second treatment plate (4), a crushing roller (6) is symmetrically arranged inside the preparation frame (1), two crushing rollers (6) are positioned above the first treatment plate (3), a connecting piece (7) which is used for connecting the crushing roller (6) is arranged inside the preparation frame (1), and a driving piece (8) which is arranged inside the preparation frame (1) is used for driving the crushing roller (6) to carry out position adjustment through the connecting piece (7), an action piece (9) which is used for controlling the crushing roller (6) to carry out crushing operation is further arranged inside the preparation frame (1), and the first treatment plate (3) is used for conveying samples with more than two sizes falling down to the second treatment plate (4) to be processed; the preparation jar body (2) is inside to be provided with axis body (10), just parallel arrangement has two regulation and control boards (11) on axis body (10), two regulation and control boards (11) are used for carrying out reprocessing work to processing board two (4) and processing board three (5), be provided with pan feeding frame (12) on preparation frame (1), and parallel arrangement has ejection of compact frame (13) on preparation jar body (2).
2. The apparatus for preparing a pathogen detection object according to claim 1, wherein: the connecting piece (7) is including setting up in inside supporting plate frame (71) of preparation frame (1), just supporting plate frame (71) both sides are provided with spacing slide rail (72) that are used for carrying out sliding connection with broken cylinder (6), and two still be connected with between spacing slide rail (72) and supporting plate frame (71) and connect grillage (73), two be provided with spacing grillage (74) on broken cylinder (6), just rotate between broken cylinder (6) and spacing grillage (74), supporting plate frame (71) inside be provided with spacing grillage (74) between carry out sliding connection's spout (75), wherein preparation frame (1) internally mounted have the baffle, and be located supporting plate frame (71) top, still install on supporting plate frame (71) and extend cylinder (76), just extend and carry out sliding connection between cylinder (76) and the baffle inner wall.
3. The apparatus for preparing a pathogen detection object according to claim 2, wherein: the driving piece (8) comprises a screw rod (81) arranged inside the preparation frame (1), two ends of the screw rod (81) penetrate through the inner wall of the preparation frame (1) and extend to the outside, a driving frame body (82) is arranged on the screw rod (81), the end part of the extending column body (76) is positioned inside the driving frame body (82), guide columns (83) which are in sliding connection with the driving frame body (82) are symmetrically arranged inside the preparation frame (1), and regulating grooves (85) are formed in the inner walls of two sides of the driving frame body (82).
4. A device for preparing a pathogen detection object according to claim 3, wherein: sliding columns (77) which are in sliding connection with the regulating and controlling grooves (85) are symmetrically arranged on the extending columns (76), and the regulating and controlling grooves (85) are composed of a descending area (86), a straight-moving area (87), an ascending area (88) and a limiting area (89).
5. The apparatus for preparing a pathogen detection object according to claim 4, wherein: the preparation frame (1) outside symmetry is installed and is protected casing (14), one of them install servo motor one (141) on protecting casing (14), just servo motor one (141) output is connected with lead screw (81) one end, rotation cylinder (142) rather than carrying out the rotation connection are installed to preparation frame (1) both sides, two protecting casing (14) are inside all to be provided with drive mechanism (143), just drive mechanism (143) both ends are installed respectively on lead screw (81) and rotation cylinder (142), wherein rotation cylinder (142) tip runs through preparation frame (1) outer wall and extends to its inside.
6. The apparatus for preparing a pathogen detection object according to claim 5, wherein: the action piece (9) is including setting up in the inside circular carousel (91) of preparation frame (1), just circular carousel (91) is connected with rotation cylinder (142) tip, just install a plurality of magnet one (92) and magnet two (93) on circular carousel (91), just the magnetism of magnet one (92) and magnet two (93) is in opposite state, wherein the angle between magnet one (92) and magnet two (93) is 90, two magnet piece (78) are all installed on spacing grillage (74), magnet one (92) produce repulsive force to magnet piece (78), just magnet two (93) produce attractive force to magnet piece (78).
7. The apparatus for preparing a pathogen detection object according to claim 6, wherein: the rotary cylinder (142) is further provided with intermittent gears (94), two rotary shafts (95) which are rotationally connected with the inner walls of the protective shells (14) are arranged in the protective shells, the rotary shafts (95) are further provided with gear bodies (96), the gear bodies (96) are located on meshing tracks of the intermittent gears (94), and the rotary shafts (95) are further provided with action bars (97).
8. The apparatus for preparing a pathogen detection object according to claim 6, wherein: the processing board I (3) both sides are all installed atress member (98), just atress member (98) are located the motion trail of action pole (97), preparation frame (1) both sides are provided with cavity (99), and cavity (99) both ends inner wall installs reset spring (90), just reset spring (90) tip is connected with atress member (98) both ends.
9. The apparatus for preparing a pathogen detection object according to claim 1, wherein: install servo motor two (15) on preparation jar body (2), just servo motor two (15) output runs through preparation jar body (2) inner wall and is connected with axis body (10), wherein equal symmetry in processing board two (4) bottom and processing board three (5) top is installed atress gyro wheel spare (151), and a plurality of atress gyro wheel spare (151) all are located the motion track of two regulation and control boards (11), wherein rotate between processing board two (4) and processing board three (5) and the preparation jar body (2) inner wall and be connected, still be connected with torsional spring (152) between processing board two (4) and processing board three (5) and the preparation jar body (2) inner wall, just all install ejection of compact mouth of pipe (153) on processing board two (4) and the processing board three (5).
10. A method for preparing a pathogen detection object based on the preparation apparatus of any one of claims 1 to 9, characterized in that: the method comprises the following steps:
s1, feeding a feeding frame (12), starting a first servo motor (141) and a second servo motor (15), rotating a screw rod (81) to enable a driving frame body (82) to perform position adjustment under the action of a guide column (83), and limiting a sliding column (77) on an extending column (76) on a regulating groove (85), namely, enabling the extending column (76) to perform descending motion, wherein the extending column (76) drives a supporting plate frame (71), a limiting sliding rail (72), a connecting plate frame (73), a crushing roller (6) and a limiting plate frame (74) to perform descending motion;
s2, a screw rod (81) drives a rotary column (142) to synchronously rotate under the action of a transmission mechanism (143), the rotary column (142) drives a first magnet (92) and a second magnet (93) to act through a circular turntable (91), and a magnet block (78) on a limiting plate frame (74) respectively moves back and forth under the action of the first magnet (92) and the second magnet (93) in the rotating process of the circular turntable (91), namely, a tissue sample is crushed and prepared;
s3, the extending column body (76) drives the supporting plate frame (71), the limiting slide rail (72), the connecting plate frame (73), the crushing roller (6) and the limiting plate frame (74) to ascend, under the meshing action of the intermittent gear (94) and the gear body (96), the action rod (97) acts on the stressed rod piece (98), the stressed rod piece (98) drives the first treatment plate (3) to adjust the position, and in the adjusting process, acting force is applied to the two reset springs (90), namely the first treatment plate (3) shakes;
s4, when the servo motor II (15) is started, the output end of the servo motor II drives the shaft body (10) to rotate, the regulating plate (11) on the shaft body (10) acts on the stressed roller piece (151), namely the treatment plate II (4) and the treatment plate III (5) perform shaking action, namely objects to be detected with different sizes are screened out, and the prepared objects to be detected are discharged by utilizing the two discharging frames (13), namely the preparation is completed.
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| CN202310822632.5A CN116532186A (en) | 2023-07-06 | 2023-07-06 | Preparation device and preparation method of pathogen detection object |
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