CN116531442A - Houttuynia cordata green tea composition with caries preventing and antibacterial activity and application thereof - Google Patents
Houttuynia cordata green tea composition with caries preventing and antibacterial activity and application thereof Download PDFInfo
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- CN116531442A CN116531442A CN202310308640.8A CN202310308640A CN116531442A CN 116531442 A CN116531442 A CN 116531442A CN 202310308640 A CN202310308640 A CN 202310308640A CN 116531442 A CN116531442 A CN 116531442A
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- green tea
- houttuynia cordata
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Classifications
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
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- A61K36/78—Saururaceae (Lizard's-tail family)
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- A—HUMAN NECESSITIES
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/335—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin
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- A61K31/352—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having six-membered rings with one oxygen as the only ring hetero atom condensed with carbocyclic rings, e.g. methantheline
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Abstract
The invention discloses a houttuynia cordata green tea composition with caries preventing and antibacterial activity and application thereof, wherein the houttuynia cordata green tea composition comprises an A component and a B component, wherein the A component is combined with the B component according to the mass ratio of 1:0.5-2, the A component is a houttuynia cordata extract, and the B component is a green tea related extract and comprises one or more of a green tea extract and tea polyphenol. The invention has the advantages of single raw material, low price, economy and safety, has the characteristic of obviously inhibiting caries main pathogenic bacteria-mutans streptococcus, can be applied to research and development of caries prevention and bacteria inhibition related pharmaceutical preparations, oral cavity washing or nursing products and the like, and is particularly suitable for meeting urgent requirements of effective, safe and long-term use in aspects of oral cavity health care and nursing.
Description
Technical Field
The invention belongs to the field of traditional Chinese medicine oral health care and prevention and treatment, and in particular relates to a mixture ratio of a cordate houttuynia and green tea composition with caries prevention and bacteriostasis activity and application thereof in caries.
Background
Caries is a progressive destruction disease of dental hard tissue mainly caused by plaque microorganism infection, and is manifested by pigmentation, sensitivity, pain, tooth defect, loosening and even shedding of teeth, and can secondarily cause complications such as pulpitis, periapical periodontitis and the like, and even affect arthritis, endocarditis and other systemic diseases. Caries has become one of the diseases that severely affects the oral function and health of humans. Streptococcus mutans is the main pathogenic bacteria of caries, and can adhere to tooth surface by water insoluble polysaccharide formed on tooth surface, and secrete acidic substances to erode enamel to cause tooth demineralization. The current common methods for clinical control of mutans streptococcus are mechanical means such as brushing teeth, rinsing mouth, and the like, and the use of fluorine-containing drugs. However, brushing is difficult to penetrate deep into the crevices and under the gingival margin and is not effective in removing plaque. Although the oral cavity washing products can effectively remove oral bacteria, most of the existing washing products contain chemical antibacterial agents such as quaternary ammonium salt, chloride, fluoride and the like, and excessive or long-term use of the chemical antibacterial agents easily causes various side effects such as bacterial resistance, oral mucosa lesions, tooth surface staining, taste change and the like. Accordingly, many natural substances of plant origin are receiving a great deal of attention as novel antibacterial agents.
Houttuynia cordata and green tea have been used and studied for many years as medicinal and edible plants. The cordate houttuynia has the chemical components of volatile oil, flavone, organic acid, alkaloid, vitamin and the like, and is considered to be pungent in taste, cold in nature and cool in nature by the traditional Chinese medicine, has the effects of clearing heat and detoxicating, promoting urination and removing dampness, invigorating stomach and promoting digestion, resolving phlegm and relieving cough and the like, and has the anti-inflammatory, antibacterial and antiviral activities of the cordate houttuynia in modern medicine. The green tea mainly comprises tea polyphenol, caffeine, mineral substances, proteins, amino acids, vitamins and other components, and the traditional Chinese medicine considers that the green tea has the effects of refreshing, clearing away heat and summer-heat, promoting digestion, resolving phlegm, clearing away heart-fire, detoxifying, reducing pathogenic fire, improving eyesight, stopping diarrhea, removing dampness and the like, and the modern medicine also proves the pharmacological effects of bacteriostasis, antioxidation, antivirus, anti-inflammatory, arteriosclerosis resistance and the like of the green tea. The research shows that the cordate houttuynia and green tea extract can effectively inhibit a plurality of bacteria such as staphylococcus aureus, bacillus dysenteriae, candida albicans, escherichia coli and the like, and has good inhibition effect on streptococcus mutans. Therefore, the houttuynia cordata extract and the green tea extract are ideal natural anticarious antibacterial compositions.
The cordate houttuynia and green tea extracts have different applications in the oral field, for example, chinese patent CN101181548A (a pharmaceutical or health-care product composition containing natural antibacterial agent) discloses a plant water-insoluble natural antibacterial substance composed of licorice, magnolia officinalis, isatis root, momordica grosvenori, tea, beet, guava leaf, bamboo leaf, white peony root, cordate houttuynia, schefflera arboricola, vine tea, honeysuckle, chrysanthemum, giant knotweed, and sarcandra glabra, and a composition formed by combining other components for oral cavity, heart, stomach intestine, joint health care or related disease treatment; chinese patent CN115212163A (a natural herbal tea lozenge and preparation thereof) discloses a natural herbal lozenge prepared from 20-38% of xylitol, 3-6% of green tea, 15-30% of alginate, 3-5% of chitosan oligosaccharide, 3-20% of compound probiotics, 8-20% of herbal composition (comprising 2-16% of peppermint, 6-8% of platycodon grandiflorum, 3-8% of lily, 4-10% of cordate houttuynia, 3-12% of ginger, 5-10% of liquorice, 4-8% of fructus momordicae, 4-10% of boat-fruited sterculia seed, 5-15% of radix puerariae, 1-5% of radix scutellariae, 2-8% of Chinese gall, 3-9% of frankincense and 2-8% of celery) and 8-16% of auxiliary materials, which are used for cleaning the oral cavity of a smoker; chinese patent CN110946804a (a mouthwash for preventing dental caries and dental ulcer and a preparation method thereof) discloses a composition comprising, by mass: 2-4 parts of marine oligosaccharide, 3-5 parts of aromatic plant extract and 20-30 parts of Chinese herbal medicine extract (the raw materials comprise 10-16 parts of green tea, 5-10 parts of boat-fruited sterculia seed, 4-6 parts of liquorice, 6-10 parts of cordate houttuynia, 3-6 parts of baikal skullcap root, 4-7 parts of purslane, 2-3 parts of moringa seed), 3-5 parts of sorbitol, 1-2 parts of sodium benzoate, 2-4 parts of xylitol, 1-2 parts of vitamin C and 40-60 parts of deionized water, wherein the mouthwash can be used for preventing dental caries and dental ulcer; chinese patent CN113143904a (an application of houttuynin analog in preparing streptococcus mutans growth and its biofilm inhibitor) discloses that sodium new houttuynin can inhibit streptococcus mutans growth and biofilm formation, and has potential in preparing streptococcus mutans growth and biofilm inhibitor or anticaries medicine. The application of cordate houttuynia and green tea extracts in the oral field in the prior art is analyzed, most of cordate houttuynia and green tea are combined with other various plant extracts for oral cleaning and bacteriostasis (comprising streptococcus mutans), and the cordate houttuynia and green tea extract has good market application prospect in oral health care and oral disease prevention, but the following defects still exist: (1) The composition contains more than ten natural plant materials, and the efficacy of the two extracts of the cordate houttuynia and the green tea cannot be well reflected; (2) In the aspect of dental caries prevention, the inhibition effect of two extracts of cordate houttuynia and green tea on streptococcus mutans cannot be highlighted due to the complexity of the composition; (3) The composition is mainly prepared into extracts according to the weight percentage of plant raw materials, and the quality or the proportion of each extract which can not be used for exerting the effect finally cannot be determined; (4) The preparation process of the plant extract is involved in each technology, but the relevant quality standard of the obtained extract is not described, and the scientificity and repeatability of the preparation of the composition and the further product transformation application can be influenced; (5) The new houttuynin sodium is a chemically synthesized houttuynin analogue, does not have the advantage of long-term use of medicinal and edible Chinese herbal medicine composition for oral health care, and has higher price compared with houttuynia cordata and green tea extracts.
In the prior art, no related application report of the mass ratio range of the two extracts of cordate houttuynia and green tea serving as anticariogenic antibacterial compositions and the inhibition effect of the two extracts on streptococcus mutans exists.
The above information disclosed in the background section is only for enhancement of understanding of the background of the invention and therefore may contain information that does not form the prior art that is already known to a person of ordinary skill in the art.
Disclosure of Invention
Aiming at the problems in the prior art, the invention aims to provide a composition with anticariogenic and antibacterial activities and application thereof, wherein the composition consists of two extracts of cordate houttuynia and green tea.
In order to achieve the above purpose, the present invention adopts the following technical scheme:
the houttuynia cordata green tea composition with caries preventing and antibacterial activity comprises an A component and a B component, wherein the A component and the B component are combined according to the mass ratio of 1:0.5-2, the A component is houttuynia cordata extract, and the B component is green tea related extract and comprises one or more of green tea extract and tea polyphenol.
In the houttuynia cordata green tea composition with caries preventing and antibacterial activity, when the component B is green tea extract, the houttuynia cordata green tea composition is a composition of houttuynia cordata extract and green tea extract in the mass ratio of 1: (0.5-2) to 2mg/mL; when the component B is tea polyphenol, the houttuynia cordata green tea composition is a composition of houttuynia cordata extract and tea polyphenol according to the mass ratio of 1:0.5-2.
In the houttuynia cordata green tea composition with caries preventing and antibacterial activity, when the houttuynia cordata extract and the green tea extract are combined according to the mass ratio of 1:1, the concentration of the houttuynia cordata extract and the green tea extract is respectively 2-20 mg/mL.
In the houttuynia cordata green tea composition with caries preventing and antibacterial activity, when the houttuynia cordata extract and the green tea extract are combined according to the mass ratio of 1:2, the concentration of the houttuynia cordata extract is 3-20 mg/mL, and the concentration of the green tea extract is 6-40 mg/mL.
In the houttuynia cordata green tea composition with caries preventing and antibacterial activity, when the houttuynia cordata extract and the green tea extract are combined according to the mass ratio of 1:0.5, the concentration of the houttuynia cordata extract is 4-20 mg/mL, and the concentration of the green tea extract is 2-10 mg/mL.
In the houttuynia cordata green tea composition with caries preventing and antibacterial activity, when the houttuynia cordata extract and the tea polyphenol are combined according to the mass ratio of 1:1, the concentration of the houttuynia cordata extract and the concentration of the tea polyphenol are respectively 2.5-10 mg/mL.
In the houttuynia cordata green tea composition with caries preventing and antibacterial activity, when the houttuynia cordata extract and the tea polyphenol are combined according to the mass ratio of 1:2, the concentration of the houttuynia cordata extract is 1-10 mg/mL, and the concentration of the tea polyphenol is 2-20 mg/mL.
In the houttuynia cordata green tea composition with caries preventing and antibacterial activity, when the houttuynia cordata extract and the tea polyphenol are combined according to the mass ratio of 1:0.5, the concentration of the houttuynia cordata extract is 3-12 mg/mL, and the concentration of the tea polyphenol is 1.5-6 mg/mL.
The houttuynia cordata green tea composition with caries preventing and antibacterial activity comprises houttuynia cordata extract, brown powder, 80 mesh granularity, no more than 5% of drying weight loss, no more than 7% of ash, no more than 20ppm of heavy metal and no more than 2ppm of pesticide residue, wherein the houttuynia cordata extract is an extract with a ratio of 10:1.
The houttuynia cordata green tea composition with caries preventing and antibacterial activity comprises green tea extract, yellow brown powder, 80 mesh granularity, no more than 5% of drying weight, no more than 7% of ash, no more than 20ppm of heavy metal and no more than 2ppm of pesticide residue, wherein the ratio of the green tea extract to the yellow brown powder is 10:1.
The houttuynia cordata green tea composition with caries preventing and antibacterial activity comprises yellow-brown powder, wherein the content of polyphenols is not less than 98.0%, the total catechin content of the characteristic components is not less than 80.0%, the epigallocatechin gallate content is not less than 40.0%, the granularity is 80 meshes, the moisture content is not more than 5%, the heavy metal content is not more than 10ppm, the lead content is not more than 2.0ppm, and the arsenic content is not more than 1.0ppm.
An application of the cordate houttuynia green tea composition in preparing anticarious antibacterial medicines.
An application of the cordate houttuynia green tea composition in preparing anticarious antibacterial oral cavity washing or nursing products.
In the application, the cordate houttuynia green tea composition is used as an effective component and is prepared into a pharmaceutical preparation with pharmaceutically acceptable auxiliary materials.
In the application, the cordate houttuynia green tea composition is used as an active ingredient and is prepared into an oral cavity washing or nursing product together with auxiliary materials acceptable on daily chemical products and disinfection products.
In the application, the pharmaceutical preparation is solution, spray, gel, lozenge or injection.
In such applications, the oral rinse or care product is a mouthwash, toothpaste, tooth powder, tooth gel, chewing gum, or an oral spray.
In the application, the pharmaceutical preparation further comprises auxiliary materials, wherein the auxiliary materials are one or more of humectant, preservative, antioxidant, emulsifier, thickener, sweetener, mucilage, aromatic and flavoring agent.
In the application, the oral cavity washing or nursing product further comprises auxiliary materials, wherein the auxiliary materials are one or more of humectant, preservative, antioxidant, emulsifier, thickener, sweetener, mucilage, aromatic and flavoring agent.
Compared with the prior art, the invention has the following beneficial effects:
(1) The houttuynia cordata green tea composition only comprises two herbal plants of houttuynia cordata and green tea, and compared with more than ten herbal plants in the prior art, the houttuynia cordata green tea composition has the advantages of relatively single raw materials, simplicity in composition, cost reduction and capability of better embodying the efficacy of the two extracts of houttuynia cordata and green tea.
(2) The quality requirements of the cordate houttuynia extract, the green tea extract or the tea polyphenol are standardized, the quality uniformity of the extracts obtained through different preparation processes or different batches can be ensured, and the scientificity and the repeatability of the composition in preparation and further conversion application are improved.
(3) The invention breaks through the limitation that the actual proportion of each extract can not be defined by combining the extracts according to the weight percentages of the raw materials in the prior art, adopts the scheme of combining the extracts according to different mass ratios, screens out proper proportioning combination and corresponding preferable action concentration, and greatly improves the scientificity of the formulation composition and efficacy evaluation of the composition.
(4) The composition focuses on the inhibition effect on streptococcus mutans, the remarkable antibacterial capability of the composition highlights the unique caries prevention and treatment potential of the composition, and a firm foundation is laid for research and development and application of related pharmaceutical preparations, oral washing or nursing products and the like.
(5) The composition belongs to medicinal and edible materials, is economical and safe, is environment-friendly, has no toxic or side effect, can meet the requirement of bacteriostasis without adding any disinfection and corrosion prevention medicines, and is particularly suitable for long-term oral health care and nursing.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings and the accompanying tables, which are used in the embodiments, will be briefly described below.
This is illustrated by the following: "HHE" represents herba Houttuyniae extract; "GTE" represents green tea extract; "TPE" stands for tea polyphenols; "0" represents negative control, BHI medium; "P" represents a positive control, 0.06% chlorhexidine gluconate; the unit is mg/mL; "-" represents sterile growth; "+" indicates the growth of bacteria.
FIG. 1 is a graph showing the effect of three extracts of example 1 alone on the growth of Streptococcus mutans; a: 10-50 mg/mL HHE; b: GTE of 10-50 mg/mL; c: 2.5-30 mg/mL TPE;
FIG. 2 is a graph showing the effect of different concentrations of HHE and GTE compositions, HHE and TPE compositions, in a 1:1 mass ratio, on Streptococcus mutans growth, respectively, in example 2; a: 0.5-5 mg/mL of a HHE and GTE composition; b: 0.25-5 mg/mL of a HHE and TPE composition;
FIG. 3 is a graph showing the effect of different compositions of HHE and GTE on Streptococcus mutans growth in examples 3-5 at mass ratios of 1:1, 1:2, and 1:0.5; a: HHE and GTE compositions in a 1:1 mass ratio in example 3, wherein HHE: 0.6-20 mg/mL, GTE: 0.6-20 mg/mL; b: HHE and GTE compositions in a mass ratio of 1:2 in example 4, wherein HHE: 0.3-20 mg/mL, GTE: 0.6-40 mg/mL; c: HHE and GTE compositions in a mass ratio of 1:0.5 in example 5, wherein HHE: 0.6-20 mg/mL, GTE: 0.3-10 mg/mL;
FIG. 4 effect of different compositions of HHE and TPE on Streptococcus mutans growth in mass ratios of 1:1, 1:2, and 1:0.5 in examples 6-8; a: HHE and TPE composition in a 1:1 mass ratio in example 6, wherein HHE: 0.12-3 mg/mL, TPE: 0.12-3 mg/mL; b: mass ratio 1 in example 7: 2 with TPE composition, wherein HHE: 0.06-1.5 mg/mL, TPE: 0.12-3 mg/mL; c: HHE and TPE composition in a mass ratio of 1:0.5 in example 8, wherein HHE: 0.25-6 mg/mL, TPE: 0.12-3 mg/mL;
FIG. 5 is a solution color of HHE and GTE different compositions in mass ratios of 1:1, 1:2, and 1:0.5 for example 9; a: different compositions of HHE and GTE; b: different compositions of HHE and TPE if a light color system is desired for product location, then 1 in fig. 5: in case 1, the leftmost composition is the lightest, of course, the product location may be other colors, such as darker colors, etc.;
FIG. 6 is a graph showing the effect of different compositions of HHE and GTE on Streptococcus mutans growth in examples 10-12 at mass ratios of 1:1, 1:2, and 1:0.5; a: HHE and GTE compositions in a 1:1 mass ratio in example 10 wherein HHE: 2-10 mg/mL, GTE: 2-10 mg/mL; b: mass ratio 1 in example 11: 2 with a GTE composition, wherein HHE: 3-10 mg/mL, GTE: 6-20 mg/mL; c: HHE and GTE compositions in a mass ratio of 1:0.5 in example 12, wherein HHE: 4-20 mg/mL, GTE: 2-10 mg/mL;
FIG. 7 is a graph showing the effect of different compositions of HHE and TPE on Streptococcus mutans growth in examples 13-15 at mass ratios of 1:1, 1:2, and 1:0.5; a: HHE and GTE compositions in a 1:1 mass ratio in example 13 wherein HHE: 2.5-10 mg/mL, TPE: 2.5-10 mg/mL; b: HHE and TPE composition in a mass ratio of 1:2 in example 14, wherein HHE: 1-8 mg/mL, TPE: 2-16 mg/mL; c: HHE and TPE composition in a mass ratio of 1:0.5 in example 15, wherein HHE: 3-12 mg/mL, TPE: 1.5-6 mg/mL.
Detailed Description
In order that the invention may be more readily understood, a further description of the invention will be rendered by reference to specific embodiments that are intended to be illustrative rather than limiting.
A herba Houttuyniae green tea composition with anticariogenic and antibacterial effects is prepared from herba Houttuyniae extract and green tea extract, or herba Houttuyniae extract and tea polyphenols by mixing and applying to preparation of anticariogenic and antibacterial products.
The bacteria used in the invention are all international standard strains, streptococcus mutans (Streptcoccus mutans, ATCC 25175), and the typical culture medium used for the bacteria is brain heart leaching broth (BHI for short). The preparation method of the BHI liquid culture medium comprises the following steps: dissolving 18.5g of BHI powder (Solarbio, LA 036) with 500mL of triple distilled water, stirring for dissolving, sterilizing at high temperature and high pressure (121.3 ℃ C., 103.4 kPa) for 30min, cooling, and sealing for later use. The preparation method of the BHI solid culture medium comprises the following steps: 500mL of triple distilled water was used to dissolve 18.5g of BHI powder (Solarbio, LA 036) and 7.5g of agar powder (Solarbio, A8190), the solution was stirred and sterilized at high temperature and high pressure (121.3 ℃ C., 103.4 kPa) for 30min, and after cooling slightly, the mixture was poured into a microbial culture dish and cooled for use. The culture conditions of the streptococcus mutans are as follows: constant temperature of 37 ℃,85% N 2 、5％CO 2 And 10% H 2 Anaerobic conditions of the mixed gas, and taking the streptococcus mutans suspension cultured to the logarithmic phase for all antibacterial experiment investigation.
Example 1:
the effect of the three extracts of houttuynia cordata, green tea and tea polyphenol on the growth of streptococcus mutans under the independent action is examined to determine the inhibition activity of different extracts on the streptococcus mutans.
100. Mu.L of final concentration 10 was added to each well of a 96-well plate 5 ～10 6 Bacterial suspensions of CFU/mL, sterile houttuynia cordata extract, green tea extract and tea polyphenols dissolved in BHI broth at different concentrations, and negative (BHI broth) and positive (0.06% chlorhexidine gluconate) controls were added and incubated for 24h under anaerobic conditions. After each hole is diluted according to a certain proportion, 5 mu L of the mixture is dripped on a BHI solid culture medium, and the mixture is cultured for 24 hours under anaerobic condition, and compared with a negative control group and a positive control group, the inhibition effect of different extracts on streptococcus mutans when the extracts act independently is observed. As shown in FIG. 1 and Table 1, A, B and C in FIG. 1 are respectively the effects of 10-50 mg/mL houttuynia cordata extract, 10-50 mg/mL green tea extract and 2.5-30 mg/mL tea polyphenol on the growth of Streptococcus mutans under the separate action, and by combining the records of the growth conditions of Streptococcus mutans in Table 1, 20mg/mL houttuynia cordata extract, 40mg/mL green tea extract and 5mg/mL tea polyphenol can obviously inhibit the growth of Streptococcus mutans. The 20mg/mL houttuynia cordata extract, 40mg/mL green tea extract and 5mg/mL tea polyphenol are determined to be the minimum inhibitory concentration, so that the subsequent scientific and reasonable preparation of the composition is facilitated.
TABLE 1 Effect of different concentrations of three extracts on S.mutans growth when acting alone
Example 2:
investigation of mass ratio 1:1 (0.5, 1, 2, 3, 4, 5 mg/mL) and green tea extract (0.5, 1, 2, 3, 4, 5 mg/mL), and the inhibition effect of different compositions of houttuynia cordata extract (0.25, 0.5, 1, 2, 4, 5 mg/mL) and tea polyphenol (0.25, 0.5, 1, 2, 4, 5 mg/mL) in mass ratio of 1:1.
As shown in FIG. 2 and Table 2, A, B of FIG. 2 is the 1:1 composition of the herba Houttuyniae extract and the green tea extract, and the effect of 0.25-5 mg/mL of the 1:1 composition of the herba Houttuyniae extract and the tea polyphenol on the growth of Streptococcus mutans, and by combining the records of the growth of Streptococcus mutans in Table 2, it is seen that the 1:1 composition of 4mg/mL of the herba Houttuyniae extract and 4mg/mL of the green tea extract and the 1:1 composition of 4mg/mL of the herba Houttuyniae extract and 4mg/mL of the tea polyphenol can significantly inhibit the growth of Streptococcus mutans, and the inhibition rates are all close to 100%. The composition of houttuynia cordata-green tea extract in a mass ratio of 1:1 is in the range of 4 to 5mg/mL and the composition of houttuynia cordata-tea polyphenol in a mass ratio of 1:1 is in the range of 4 to 5mg/mL, compared with the independent action of the different extracts in example 1, has more remarkable mutans streptococcus inhibitory activity.
TABLE 2 influence of HHE and GTE or TPE compositions in a 1:1 mass ratio on S.mutans growth
Example 3:
the inhibition effect of different compositions of houttuynia cordata extract (0.6, 1.2, 2.5, 5, 10, 20 mg/mL) and green tea extract (0.6, 1.2, 2.5, 5, 10, 20 mg/mL) in mass ratio of 1:1 on streptococcus mutans was examined. As shown in FIG. 3A and Table 3, it was found that 1:1 combination of 2.5mg/mL houttuynia cordata extract and 2.5mg/mL green tea extract significantly inhibited Streptococcus mutans growth with an inhibition rate approaching 100%. Therefore, the houttuynia cordata-green tea extract composition with the mass ratio of 1:1 can obviously inhibit streptococcus mutans in the range of 2.5-20 mg/mL.
TABLE 3 Effect of HHE and GTE compositions of different Mass ratios on S.mutans growth
Example 4:
the inhibition effect of different compositions of houttuynia cordata extract (0.3, 0.6, 1.2, 2.5, 5, 10, 20 mg/mL) and green tea extract (0.6, 1.2, 2.5, 5, 10, 20, 40 mg/mL) in mass ratio of 1:2 on mutans streptococcus was examined. As shown in FIG. 3B and Table 3, it was found that 1:2 combination of 5mg/mL houttuynia cordata extract and 10mg/mL green tea extract significantly inhibited S.mutans growth with an inhibition rate approaching 100%. Therefore, the houttuynia cordata-green tea extract composition with the mass ratio of 1:2 can obviously inhibit streptococcus mutans when the houttuynia cordata extract is 5-20 mg/mL and the green tea extract is 10-40 mg/mL.
Example 5:
the inhibition effect of different compositions of houttuynia cordata extract (0.6, 1.2, 2.5, 5, 10, 20 mg/mL) and green tea extract (0.3, 0.6, 1.2, 2.5, 5, 10 mg/mL) in mass ratio of 1:0.5 on Streptococcus mutans was examined. As shown in FIG. 3C and Table 3, it was found that 1:0.5 composition of 5mg/mL houttuynia cordata extract and 2.5mg/mL green tea extract significantly inhibited Streptococcus mutans growth with an inhibition rate approaching 100%. Therefore, the composition of the houttuynia cordata-green tea extract with the mass ratio of 1:0.5 can obviously inhibit the streptococcus mutans when the houttuynia cordata extract is 5-20 mg/mL and the green tea extract is 2.5-10 mg/mL.
Example 6:
the inhibition effect of different compositions of houttuynia cordata extracts (0.12, 0.25, 0.5, 1, 2, 3 mg/mL) and tea polyphenol (0.12, 0.25, 0.5, 1, 2, 3 mg/mL) in a mass ratio of 1:1 on streptococcus mutans was examined. As shown in FIG. 4A and Table 4, it was found that 1:1 combination of 3mg/mL houttuynia cordata extract and 3mg/mL tea polyphenol significantly inhibited Streptococcus mutans growth with an inhibition rate approaching 100%. Therefore, the houttuynia cordata-tea polyphenol composition with the mass ratio of 1:1 can remarkably inhibit streptococcus mutans when the houttuynia cordata extract is 3mg/mL and the tea polyphenol is 3 mg/mL.
TABLE 4 influence of different mass ratios of HHE to TPE compositions on S.mutans growth
Example 7:
the inhibition effect of different compositions of houttuynia cordata extract (0.06, 0.12, 0.25, 0.5, 1, 1.5 mg/mL) and tea polyphenol (0.12, 0.25, 0.5, 1, 2, 3 mg/mL) in mass ratio of 1:2 on mutans streptococcus was examined. The results are shown in FIG. 4B and Table 4, and can be seen at 1mg/mL houttuynia cordata extract with 1 of tea polyphenols at 2 mg/mL: the composition 2 can obviously inhibit the growth of the streptococcus mutans, and the inhibition rate is close to 100%. Therefore, the houttuynia cordata-tea polyphenol composition with the mass ratio of 1:2 can obviously inhibit streptococcus mutans when the houttuynia cordata extract is 1-1.5 mg/mL and the tea polyphenol is 2-3 mg/mL.
Example 8:
the inhibition effect of different compositions of cordate houttuynia extract (0.25, 0.5, 1, 2, 4, 6 mg/mL) and tea polyphenol (0.12, 0.25, 0.5, 1, 2, 3 mg/mL) with the mass ratio of 1:0.5 on streptococcus mutans was examined. As shown in FIG. 4C and Table 4, it was found that 1:0.5 composition of 4mg/mL houttuynia cordata extract and 2mg/mL tea polyphenol significantly inhibited the growth of Streptococcus mutans with an inhibition rate approaching 100%. Therefore, the houttuynia cordata-tea polyphenol composition with the mass ratio of 1:0.5 can obviously inhibit streptococcus mutans when the houttuynia cordata extract is 4-6 mg/mL and the tea polyphenol is 2-3 mg/mL.
Example 9:
preparing herba Houttuyniae-green tea extract composition and herba Houttuyniae-tea polyphenols composition at mass ratio of 1:1, 1:2 and 1:0.5. Wherein, the houttuynia cordata extract concentration in the houttuynia cordata-green tea extract composition is 2-20 mg/mL, and the green tea extract concentration is 2-20 mg/mL in the houttuynia cordata-green tea extract composition with the mass ratio of 1:1; the houttuynia cordata extract concentration in the houttuynia cordata-green tea extract composition is 3-20 mg/mL, and the green tea extract concentration is 6-40 mg/mL in a mass ratio of 1:2; the houttuynia cordata extract concentration in the houttuynia cordata-green tea extract composition is 4-20 mg/mL, and the green tea extract concentration is 2-10 mg/mL in the mass ratio of 1:0.5; the concentration of the houttuynia cordata extract in the houttuynia cordata-tea polyphenol composition is 2.5-10 mg/mL, and the concentration of the tea polyphenol is 2.5-10 mg/mL in the houttuynia cordata-tea polyphenol composition with the mass ratio of 1:1; the concentration of the houttuynia cordata extract in the houttuynia cordata-tea polyphenol composition is 1-10 mg/mL, and the concentration of the tea polyphenol is 2-20 mg/mL in the houttuynia cordata-tea polyphenol composition with the mass ratio of 1:2; the houttuynia cordata extract concentration in the houttuynia cordata-tea polyphenol composition is 3-12 mg/mL, and the tea polyphenol concentration is 1.5-6 mg/mL in the houttuynia cordata-tea polyphenol composition with the mass ratio of 1:0.5. Observing the dissolution and color of solutions of different compositions can facilitate selection of suitable compositions based on the location of the product, e.g., lower concentration compositions may be considered if cost is a priority.
Example 10:
investigation of mass ratio 1:1 (2, 3, 4, 5, 6, 7, 8, 9, 10 mg/mL) and green tea extract (2, 3, 4, 5, 6, 7, 8, 9, 10 mg/mL). As shown in FIG. 6A and Table 5, it was found that 1:1 combination of 2mg/mL houttuynia cordata extract and 2mg/mL green tea extract significantly inhibited S.mutans growth with an inhibition rate approaching 100%. Therefore, the composition of the houttuynia cordata-green tea extract with the mass ratio of 1:1 can obviously inhibit the streptococcus mutans in the range of 2-10 mg/mL.
TABLE 5 influence of HHE and GTE compositions of different Mass ratios on S.mutans growth
Example 11:
the inhibition effect of different compositions of houttuynia cordata extract (3, 4, 5, 6, 7, 8, 9, 10 mg/mL) and green tea extract (6, 8, 10, 12, 14, 16, 18, 20 mg/mL) in mass ratio of 1:2 on streptococcus mutans was examined. As shown in FIG. 6B and Table 5, it was found that 1:2 combination of 3mg/mL houttuynia cordata extract and 6mg/mL green tea extract significantly inhibited S.mutans growth with an inhibition rate approaching 100%. Therefore, the houttuynia cordata-green tea extract composition with the mass ratio of 1:2 can obviously inhibit streptococcus mutans when the houttuynia cordata extract is 3-10 mg/mL and the green tea extract is 6-20 mg/mL.
Example 12:
the inhibition effect of different compositions of houttuynia cordata extract (4, 5, 6, 7, 8, 9, 10, 20 mg/mL) and green tea extract (2, 2.5, 3, 3.5, 4, 4.5, 5, 10 mg/mL) in mass ratio of 1:0.5 on mutans streptococcus was examined. As shown in FIG. 6C and Table 5, it was found that 1:0.5 composition of 4mg/mL houttuynia cordata extract and 2mg/mL green tea extract significantly inhibited S.mutans growth with an inhibition rate approaching 100%. Therefore, the composition of the houttuynia cordata-green tea extract with the mass ratio of 1:0.5 can obviously inhibit the streptococcus mutans when the houttuynia cordata extract is 4-20 mg/mL and the green tea extract is 2-10 mg/mL.
Example 13:
the inhibition effect of different compositions of houttuynia cordata extract (2.5, 3, 4, 5, 6, 7, 8, 9, 10 mg/mL) and tea polyphenol (2.5, 3, 4, 5, 6, 7, 8, 9, 10 mg/mL) in mass ratio of 1:1 on mutans streptococcus was examined. As shown in FIG. 7A and Table 6, it was found that 1:1 combination of 2.5mg/mL houttuynia cordata extract and 2.5mg/mL tea polyphenol significantly inhibited Streptococcus mutans growth with an inhibition rate approaching 100%. Therefore, the houttuynia cordata-tea polyphenol composition with the mass ratio of 1:1 can obviously inhibit streptococcus mutans when the houttuynia cordata extract is 2.5-10 mg/mL and the tea polyphenol is 2.5-10 mg/mL.
TABLE 6 influence of different mass ratios of HHE to TPE compositions on S.mutans growth
Example 14:
the inhibition effect of different compositions of houttuynia cordata extracts (1, 1.5, 2, 3, 4, 5, 6, 7, 8 mg/mL) and tea polyphenols (2, 3, 4, 6, 8, 10, 12, 14, 16 mg/mL) in mass ratio of 1:2 on mutans streptococcus was examined. As shown in FIG. 7B and Table 6, it was found that 1:2 combination of 1mg/mL houttuynia cordata extract and 2mg/mL tea polyphenol significantly inhibited S.mutans growth with an inhibition rate approaching 100%. Therefore, the houttuynia cordata-tea polyphenol composition with the mass ratio of 1:2 can obviously inhibit streptococcus mutans when the houttuynia cordata extract is 1-8 mg/mL and the tea polyphenol is 2-16 mg/mL.
Example 15:
the inhibition effect of different compositions of houttuynia cordata extracts (3, 4, 5, 6, 7, 8, 9, 10, 12 mg/mL) and tea polyphenols (1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, 6 mg/mL) in mass ratio of 1:0.5 on mutans streptococcus was examined. As shown in FIG. 7C and Table 6, it was found that 1:0.5 composition of 3mg/mL houttuynia cordata extract and 1.5mg/mL tea polyphenol significantly inhibited the growth of Streptococcus mutans with an inhibition rate of approximately 100%. Therefore, the houttuynia cordata-tea polyphenol composition with the mass ratio of 1:0.5 can obviously inhibit streptococcus mutans when the houttuynia cordata extract is 3-12 mg/mL and the tea polyphenol is 1.5-6 mg/mL.
Based on all the above examples, it is finally preferred that:
the houttuynia cordata green tea composition with caries preventing and bacteriostasis activity comprises a composition of houttuynia cordata extract and green tea extract in the mass ratio of 1:0.5-2 or a composition of houttuynia cordata extract and tea polyphenol in the mass ratio of 1:0.5-2.
More preferably, when the cordate houttuynia extract and the green tea extract are mixed according to the mass ratio of 1:1, the preferred concentrations are: houttuynia cordata extract: 2. 2.5, 3, 4, 5, 6, 7, 8, 9, 10, 15mg/mL; green tea extract: 2. 2.5, 3, 4, 5, 6, 7, 8, 9, 10, 15mg/mL; when the cordate houttuynia extract and the green tea extract are mixed according to the mass ratio of 1:2, the preferred concentrations are: houttuynia cordata extract: 3.4, 5, 6, 7, 8, 9, 10mg/mL; green tea extract: 6. 8, 10, 12, 14, 16, 18, 20mg/mL; when the cordate houttuynia extract and the green tea extract are combined according to the mass ratio of 1:0.5, the preferable concentration is as follows: houttuynia cordata extract: 4.5, 6, 7, 8, 9, 10, 20mg/mL; green tea extract: 2. 2.5, 3, 3.5, 4, 4.5, 5, 10mg/mL; when the cordate houttuynia extract and tea polyphenol are mixed according to the mass ratio of 1:1, the preferred concentrations are: houttuynia cordata extract: 2.5, 3, 4, 5, 6, 7, 8, 9, 10mg/mL; green tea extract: 2.5, 3, 4, 5, 6, 7, 8, 9, 10mg/mL; when the cordate houttuynia extract and tea polyphenol are combined according to the mass ratio of 1:2, the preferable concentration is as follows: houttuynia cordata extract: 1. 1.5, 2, 3, 4, 5, 6, 7, 8, 10mg/mL; green tea extract: 2.5, 3, 4, 5, 6, 7, 8, 9, 10, 20mg/mL; when the cordate houttuynia extract and tea polyphenol are combined according to the mass ratio of 1:0.5, the preferable concentration is as follows: houttuynia cordata extract: 3.4, 5, 6, 7, 8, 10, 12mg/mL; green tea extract: 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, 6mg/mL.
Meanwhile, the quality requirements of the cordate houttuynia extract, the green tea extract and the tea polyphenol are standardized, and the scientificity of the composition formula composition and the efficacy evaluation is greatly improved. The composition has the advantages of homology of medicine and food, natural and harmless property, single raw material, low price, economy and safety, has the characteristic of obviously inhibiting main pathogenic bacteria of caries-streptococcus mutans, can be applied to research and development of caries prevention and bacteria inhibition related pharmaceutical preparations, oral cavity washing or nursing and other products, and is particularly suitable for meeting urgent requirements of effective, safe and long-term use in aspects of oral cavity health care and nursing.
The quality requirement of the adopted houttuynia cordata extract is 10:1 proportion of extract, brown powder, 80 meshes of particles, no more than 5 percent of drying weight loss, no more than 7 percent of ash, no more than 20ppm of heavy metal and no more than 2ppm of pesticide residue; the quality requirements of the adopted green tea extract are 10:1 proportion of extract, yellow brown powder, granularity of 80 meshes, loss on drying of no more than 5%, ash content of no more than 7%, heavy metal of no more than 20ppm and pesticide residue of no more than 2ppm; the quality of the adopted tea polyphenol is required to be yellow-brown powder, the tea polyphenol has peculiar smell, the polyphenol content is not less than 98.0%, the total catechin of the characteristic components is not less than 80.0%, the epigallocatechin gallate is not less than 40.0%, the granularity is 80 meshes, the moisture is not more than 5%, the heavy metal is not more than 10ppm, the lead is not more than 2.0ppm, and the arsenic is not more than 1.0ppm. This is the quality requirement of the relevant component.
The houttuynia cordata extract, the green tea extract and the tea polyphenol can be extracted from houttuynia cordata and green tea by a solvent extraction method, an ultrasonic extraction method, a supercritical extraction method or other methods, so long as the quality requirements can be met.
The above embodiments are merely preferred embodiments of the present invention, which are used to illustrate the technical solution of the present invention, but not to limit it in any way. All other embodiments obtained by those skilled in the art without creative efforts or modifications or substitutions made by the technical spirit of the invention, such as modifications to the technical solutions described in the foregoing embodiments, equivalent substitutions of some technical features thereof, etc., still belong to the protection scope of the technical solutions of the present invention.
Claims (10)
1. The houttuynia cordata green tea composition with caries preventing and antibacterial activity is characterized by comprising an A component and a B component, wherein 1-20 mg/mL of the A component is combined with the B component according to the mass ratio of 1:0.5-2, the A component is houttuynia cordata extract, and the B component is green tea related extract and comprises one or more of green tea extract and tea polyphenol.
2. The houttuynia cordata green tea composition with anticariogenic activity according to claim 1, wherein preferably, when the component B is green tea extract, the houttuynia cordata green tea composition is a composition of houttuynia cordata green tea extract and green tea extract in a mass ratio of 1: (0.5-2) to 2mg/mL; when the component B is tea polyphenol, the houttuynia cordata green tea composition is a composition of houttuynia cordata extract and tea polyphenol according to the mass ratio of 1:0.5-2.
3. The green tea composition of claim 1, wherein the concentrations of the extract of houttuynia cordata and the extract of green tea are 2-20 mg/mL, respectively, when the extract of houttuynia cordata and the extract of green tea are combined according to a mass ratio of 1:1.
4. The green tea composition of claim 1, wherein the concentrations of the houttuynia cordata extract and the tea polyphenol are respectively 2.5-10 mg/mL when the houttuynia cordata extract and the tea polyphenol are combined according to a mass ratio of 1:1.
5. Use of the houttuynia cordata green tea composition according to any one of claims 1-4 for preparing anticarious antibacterial drugs.
6. Use of a houttuynia cordata green tea composition as claimed in any one of claims 1-4 for the preparation of anticarious antibacterial oral wash or care products.
7. The use according to claim 5, wherein the houttuynia cordata green tea composition is formulated as a pharmaceutical preparation with pharmaceutically acceptable excipients as an active ingredient.
8. The use according to claim 6, wherein the houttuynia cordata green tea composition is formulated into an oral wash or care product as an active ingredient together with an auxiliary material acceptable on daily chemical products and on disinfection products.
9. The use according to claim 7, wherein the pharmaceutical formulation is a solution, spray, gel, lozenge or injection.
10. The use according to claim 8, wherein the oral wash or care product is a mouthwash, toothpaste, tooth powder, tooth gel, chewing gum or an oral spray.
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| CN2022112328789 | 2022-10-09 | ||
| CN202211232878.9A CN115444799A (en) | 2022-10-09 | 2022-10-09 | A herba Houttuyniae wash product for preventing caries and inhibiting bacteria and production line thereof |
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| CN202211232878.9A Withdrawn CN115444799A (en) | 2022-10-09 | 2022-10-09 | A herba Houttuyniae wash product for preventing caries and inhibiting bacteria and production line thereof |
| CN202310308640.8A Pending CN116531442A (en) | 2022-10-09 | 2023-03-27 | Houttuynia cordata green tea composition with caries preventing and antibacterial activity and application thereof |
| CN202310305414.4A Pending CN116421515A (en) | 2022-10-09 | 2023-03-27 | Houttuynia cordata washing product for caries prevention and bacteriostasis and production line thereof |
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