CN116530615A

CN116530615A - Seaweed treatment method

Info

Publication number: CN116530615A
Application number: CN202310310878.4A
Authority: CN
Inventors: 唐峰; 张银照; 周露阳; 周龙锡
Original assignee: Zhejiang Fengyu Marine Biological Products Co ltd
Current assignee: Zhejiang Fengyu Marine Biological Products Co ltd
Priority date: 2023-03-28
Filing date: 2023-03-28
Publication date: 2023-08-04

Abstract

The invention provides a seaweed treatment method, which comprises the following steps: s1, cleaning seaweed raw materials, then air-drying, crushing to obtain seaweed powder, and steaming to obtain seaweed solution; s2, squeezing and filtering the seaweed solution by using a filter press to obtain filtrate and filter residues, adding complex enzyme into the filtrate, carrying out enzymolysis for 10-12 hours to obtain enzymolysis liquid, and centrifuging to obtain supernatant; s3, separating the supernatant by using an ultrafiltration membrane to obtain trapped fluid, and desalting by electrodialysis to obtain desalted fluid; s4, concentrating the desalted liquid in vacuum to obtain a concentrate, and drying at low temperature to obtain a seaweed extract; s5, drying the filter residues to obtain seaweed residues, and mixing the seaweed residues with the enzymolysis squid paste, the marine fish protein peptide, the auxiliary agent and deionized water to obtain a mixture; s6, drying the mixture, crushing and sieving to obtain the compound seaweed powder. The invention can realize the high-efficiency enzymolysis of the seaweed and the comprehensive recycling of the seaweed residues after the active ingredients are extracted, not only can solve the problem of environmental pollution caused by the spoilage of the seaweed, but also can realize economic benefits, and has better economic and social values.

Description

Seaweed treatment method

Technical Field

The invention relates to a seaweed treatment method.

Background

Seaweed is a collective term for marine Algae (Algae) and is rich in a variety of nutrients including polysaccharides, mannitol, a variety of natural plant hormones, and the like. The seaweed fertilizer is a soluble fertilizer containing alginic acid, which is prepared by extracting essence substances in seaweed by using natural seaweed as a raw material through a special physiological and biochemical process means, and comprises seaweed concentrate, seaweed extract powder and the like. The common seaweed fertilizer raw materials in the market mainly comprise Ascophyllum nodosum (Ascophyllum nodosum), thallus laminariae (Ecklonia maxima), herba Zosterae Marinae (Laminaria digitata & Laminaria japonica), sargassum (Sargassum wightii) and the like in brown algae. Over 200 kinds of gulfweed exist in the world, and nearly 100 kinds of gulfweed exist in China, but at present, agricultural research on gulfweed resources in China is lagged, and the resources are not fully developed.

It has been demonstrated that auxins in seaweed promote the formation of larger root systems in plants to enhance the utilization of soil nutrients and moisture by the roots; the sieve tube cells of the stem part are increased, the photosynthetic products are accelerated to be transported to other parts from the leaves, and the stress resistance, the disease resistance, the insect resistance and the lodging resistance of the plants are enhanced, so that the purposes of increasing yield and efficiency are achieved. Studies have shown that: the yield increase rate of the cucumber sprayed with the seaweed fertilizer is 31.9%; the tomato root is irrigated with liquid seaweed fertilizer to increase the yield by 26.8% compared with the control; the yield of the pepper is increased by 10.10 percent compared with the control by irrigating the root with liquid seaweed fertilizer; the yield of the potato is increased by 16.8% compared with the contrast; the yield of the liquid seaweed fertilizer sprayed on the lettuce is increased by 114.3 percent compared with the control. In addition, the sea research institute Han Lijun of China academy of sciences and the like proves that the germination rate is obviously improved by soaking cabbage seeds with seaweed extract, the growth promoting effect and the yield increasing effect are obvious, the yield can be increased by 50% at most, and the drought resisting effect is obvious.

Seaweed as a feed additive was studied starting in the 50 s of the 20 th century. In China, the application of seaweed meal as a feed additive just begins, and related enterprises are not many, and the attention of the feed enterprises is not high. Therefore, the comprehensive utilization of the low-value seaweed to develop the functional feed has wide market space.

The marine algae contains rich nutrient substances, and the quality of the cultured products can be improved and the growth performance of the cultured varieties can be improved by adding the marine algae into the feed according to a certain proportion, so that a plurality of reports are already reported in academic researches on animal nutrition: the seaweed meal with the content of 2-6% is added into the feed for the laying hen, the iodine content of the produced high-iodine egg is more than ten times higher than that of a common egg, and the vitamin A content is 17-46% higher than that of the common egg; the seaweed meal is added into the pig feed for growing and fattening, so that the daily gain is improved by 18%, the feed-meat ratio is 3:1, the concentrated feed is saved by about 4%, the occurrence of diseases such as escherichia coli and enteritis of pigs is reduced, the fat structure of meat is changed, and the content of cholesterol is reduced; the addition of 5% seaweed meal into fish feed can increase the intake of fish to bait, increase the daily gain of fish by 11%, reduce the occurrence of albinism and other diseases, reduce the incidence rate by more than 95%, and shorten the cultivation period by more than one month.

The feeding value of the seaweed mainly exists in iodides, minerals, vitamins and unknown active substances which stimulate the growth of animals, particularly, the nutrients in the seaweed exist in an organic state, so that the seaweed is easy to absorb by the animals. The mechanism of action of the feed has (1) promoting animal growth, the bioactive substances can regulate the nutrition balance of the feed, and improve the metabolism, digestion and absorption of the animal on the nutrient substances, such as vitamin E (tocopherol) in seaweed, and can improve the egg yield and fertilization rate of the animal; algal polysaccharides control cell division, regulate cell growth, and maintain normal metabolism of organisms. (2) The terpenes are the most representative secondary metabolites of the seaweed, mainly halogenated or aromatic terpenes, and have the biological activity functions of killing and inhibiting bacterial growth, resisting tumors and the like; in addition, seaweed is rich in superoxide dismutase (SOD), is a high-efficiency substance for removing oxygen free radical agent in vivo, and protects cells and tissues from oxidative damage. (3) Improving animal quality such as phycocyanin, iodine, sodium, etc., making animal skin and muscle bright, delicious, and properly reducing cholesterol content of the product. (4) Most seaweeds contain a certain amount of biological jelly, such as seaweed gel, fucoidin and other sticky jelly, so that the seaweed gel can become a natural high-quality adhesive for the fish and shrimp feed after processing, and can enhance the stability of the fish and shrimp feed and prevent water pollution.

In conclusion, the marine algae is a feed resource with great research value, economic value and development and utilization potential. Today, where food safety is increasingly important, with public concerns about food safety and environmental-friendly feeding demands, seaweed, a natural and green feed resource, is expected to be widely used in industrial aquaculture production.

Disclosure of Invention

The technical problem to be solved by the invention is to provide the seaweed treatment method, which can realize the efficient enzymolysis of seaweed and the comprehensive recycling of the seaweed residues after the active ingredients are extracted, can solve the environmental pollution problem caused by the seaweed spoilage, can realize economic benefit, and has better economic and social values.

In order to solve the technical problems, the technical scheme of the invention is as follows:

a method for processing seaweed, comprising the steps of:

s1, cleaning seaweed raw materials, air-drying, crushing by a crusher, sieving to obtain seaweed powder, adding the seaweed powder into distilled water, heating to 75 ℃, and steaming for 2-3 hours to obtain seaweed solution;

s2, squeezing and filtering the seaweed solution obtained in the step S1 by using a filter press to obtain filtrate and filter residues, adding complex enzyme into the filtrate, adjusting the pH value to 5, heating to 45 ℃ and performing enzymolysis for 10-12 hours to obtain an enzymolysis solution, and centrifuging the enzymolysis solution for 5-10 minutes to obtain a supernatant;

s3, separating the supernatant obtained in the step S2 by using an ultrafiltration membrane to obtain a trapped fluid, and carrying out electrodialysis desalination on the trapped fluid to obtain a desalted fluid;

s4, concentrating the desalted liquid obtained in the step S3 in vacuum for 1-2 hours to obtain concentrate, and drying the concentrate at low temperature for 2-3 hours to obtain seaweed extract;

s5, drying the filter residue obtained in the step S2 for 2-3 hours to obtain seaweed residues, mixing the seaweed residues, the enzymolysis squid paste, the marine fish protein peptide, the auxiliary agent and the deionized water, and stirring until the mixture is uniform to obtain a mixture;

s6, drying the mixture obtained in the step S5 for 3-4 hours by using a rotary grinding drying tower, crushing by using a crusher, and sieving to obtain the compound seaweed powder.

Further, in the step S1, a 50-mesh sieve is used for sieving, and the weight ratio of the seaweed powder to the distilled water is 1:15.

Further, in the step S2, the complex enzyme consists of cellulase, pectase and algin lyase in a weight ratio of 2:2:1, wherein the weight ratio of the complex enzyme to the filtrate is 1:125, and the rotating speed during centrifugation is 5000rpm.

Further, in the step S3 of the invention, the molecular weight cut-off of the ultrafiltration membrane is 1000Da, the operating voltage during electrodialysis desalination is 30V, and the loading flow rate is 50L/hour.

Further, in the step S4, the temperature during vacuum concentration is 60 ℃ and the vacuum degree is 0.09Mpa; the temperature at the time of low-temperature drying was 30 ℃.

Further, in step S5 of the present invention, the temperature at the time of drying is 50 ℃; according to the weight portions, 5 to 10 portions of seaweed residues, 3 to 6 portions of enzymolysis squid paste, 2 to 5 portions of marine fish protein peptide, 0.5 portion of auxiliary agent and 20 to 30 portions of deionized water.

Further, in the step S5 of the present invention, the auxiliary agent is prepared by the following steps:

adding hydroxy polyethylene glycol acrylate, N-vinyl pyrrolidone, absolute ethyl alcohol and deionized water into a reaction bottle, stirring under the protection of nitrogen, heating to 60 ℃, adding azodiisobutyronitrile into the reaction bottle, carrying out heat preservation and stirring reaction for 6 hours to obtain a reaction solution, cooling the reaction solution to room temperature, centrifuging for 5 minutes to obtain a solid, washing the solid with absolute ethyl alcohol for 3 times, and then carrying out vacuum drying to constant weight to obtain the auxiliary agent.

Further, in the preparation step of the auxiliary agent, the weight ratio of the hydroxyl polyethylene glycol acrylate to the N-vinyl pyrrolidone to the absolute ethyl alcohol to the deionized water to the azodiisobutyronitrile is 8:19:5:2:0.001, the rotating speed during centrifugation is 8000rpm, and the temperature during vacuum drying is 40 ℃.

Further, in the step S6, the drying temperature of the rotary grinding drying tower is 50 ℃, and a 50-mesh sieve is used for sieving.

Compared with the prior art, the invention has the following beneficial effects:

1) Alginic acid is mainly present in the cell wall of seaweed and is a polysaccharide composed of beta-D-mannuronic acid (M) and alpha-L-guluronic acid (G) in an uneven proportion, and has a molecular weight ranging from 1 to 60 tens of thousands, is substantially insoluble in water and organic solvents, and is easily soluble in alkaline solutions. The traditional physicochemical method such as acid pretreatment, alkali extraction, precipitation drying and the like is a mature process in extracting alginic acid, but the required temperature is higher, the time is longer, the damage of active ingredients is larger, the reaction condition of extracting alginic acid by an enzymolysis method is milder, the yield is high, the environmental protection pressure is smaller, and the defects of high cost and difficult control of the condition are overcome. Therefore, the method adopts a step method to extract alginic acid in seaweed, firstly adopts a mild physical method to pretreat the seaweed raw material, damages the cell wall structure, and then carries out enzymolysis by complex enzyme, thereby effectively improving the extraction rate of active ingredients in the seaweed raw material.

2) The purpose of separating and purifying the zymolyte is to obtain a product with high purity or high activity and to maximize the yield. As the content of the biological active substances such as alginic acid and the like in a production reaction system is low, the separation effect of the traditional separation technology is often not ideal, and therefore, the invention adopts the ultrafiltration membrane separation technology, and has the advantages of high efficiency, simple operation, no pollution and the like, and the content of alginate in the seaweed extract prepared by the invention is more than or equal to 20 percent through tests; in addition, the invention also adopts electrodialysis desalination technology to carry out desalination treatment on the seaweed extract, and the content of water-soluble chloride (calculated by sodium chloride) in the seaweed extract prepared by the invention is less than or equal to 10 percent through test.

3) The current academy considers that high-temperature drying often causes nutrient destruction and loss, and even some toxic and harmful substances can be generated, so that the invention firstly utilizes vacuum conditions to reduce the evaporation boiling point of moisture, realizes the rapid concentration of materials in vacuum, effectively protects the functionality of active substances, and then utilizes strong convection of hot air suitable for drying to realize the low-temperature rapid drying of the materials. The seaweed raw material containing high moisture and high fiber is not subjected to high-temperature and high-pressure drying treatment, directly enters the downstream industrial end, better preserves the effectiveness of various nutritional ingredients and the physiological activity of heat-sensitive substances in the raw material, and finally improves the effective functionality of the series products.

4) The seaweed contains a large amount of insoluble cellulose, so that the active ingredients are difficult to extract completely and remain in seaweed residues. In order to realize the comprehensive utilization of the low-value seaweed, the seaweed slag generated after the extraction of part of active substances is compounded with products such as enzymolysis squid paste and marine fish protein peptide processed by low-value aquatic products or aquatic product processing byproducts according to a proportion to prepare the compound seaweed powder product. Early-stage researches show that seaweed residues after active substances are extracted still have obvious growth promoting effect on cultured animals, and the feeding promoting effect of enzymolysis squid paste, marine fish protein peptide and the like on the cultured animals is highly accepted in the market, so that the prepared compound seaweed powder product has better growth promoting effect on the cultured animals, has better feeding value and realizes the comprehensive utilization of low-value seaweed; in addition, the invention also adopts a rotary grinding and drying technology in the preparation process of the compound seaweed powder, realizes the quick drying of the product, effectively avoids the loss of active ingredients and the damage of nutrient substances, and the content of total sugar in the compound seaweed powder prepared by the invention is more than or equal to 30g/kg and the content of crude protein is more than or equal to 35 percent through tests.

5) The compatibility among the seaweed residues, the enzymolysis squid paste and the marine fish protein peptide is not ideal, so the invention also adds an auxiliary agent prepared by polymerization reaction of hydroxy polyethylene glycol acrylic ester, N-vinyl pyrrolidone and the like, and the auxiliary agent can effectively improve the compatibility among the seaweed residues, the enzymolysis squid paste and the marine fish protein peptide, thereby further improving the growth promoting effect of the compound seaweed powder on cultured animals.

Detailed Description

The present invention will be described in detail with reference to specific examples, wherein the exemplary embodiments and descriptions of the present invention are provided for the purpose of illustration and are not intended to be limiting.

Example 1:

the seaweed was treated according to the following steps:

s1, cleaning seaweed raw materials, then air-drying, crushing the seaweed raw materials by a crusher, sieving the seaweed powder by a 50-mesh sieve to obtain seaweed powder, adding the seaweed powder into distilled water, heating the seaweed powder to 75 ℃ and then steaming the seaweed powder for 2.5 hours to obtain a seaweed solution, wherein the weight ratio of the seaweed powder to the distilled water is 1:15;

s2, squeezing and filtering the seaweed solution obtained in the step S1 by using a filter press to obtain filtrate and filter residues, adding complex enzyme consisting of cellulase, pectase and algin lyase in a weight ratio of 2:2:1 into the filtrate, adjusting the pH value to 5, heating to 45 ℃, performing enzymolysis for 11 hours to obtain enzymolysis liquid, and centrifuging the enzymolysis liquid at a rotation speed of 5000rpm for 8 minutes to obtain supernatant;

s3, separating the supernatant obtained in the step S2 by using an ultrafiltration membrane with the molecular weight cutoff of 1000Da to obtain a trapped fluid, and carrying out electrodialysis desalination on the trapped fluid to obtain a desalted fluid, wherein the operation voltage during electrodialysis desalination is 30V, and the loading flow rate is 50L/hour;

s4, concentrating the desalted liquid obtained in the step S3 in vacuum at 60 ℃ and 0.09Mpa vacuum degree for 1.5 hours to obtain a concentrate, and drying the concentrate at 30 ℃ for 2.5 hours to obtain the seaweed extract;

s5, drying the filter residue obtained in the step S2 at 50 ℃ for 2.5 hours to obtain seaweed residues, mixing the seaweed residues, the enzymolysis squid paste, the marine fish protein peptide, the auxiliary agent and the deionized water, and stirring until the seaweed residues, the enzymolysis squid paste, the marine fish protein peptide, the auxiliary agent and the deionized water are uniformly mixed to obtain a mixture, wherein the seaweed residues are 8 parts, the enzymolysis squid paste is 4 parts, the marine fish protein peptide is 3 parts, the auxiliary agent is 0.5 part and the deionized water is 25 parts by weight;

s6, drying the mixture obtained in the step S5 for 3.5 hours at 50 ℃ by using a rotary grinding drying tower, crushing by using a crusher, and sieving by using a 50-mesh sieve to obtain the compound seaweed powder.

The auxiliary agent in the step S5 is prepared by the following steps:

adding hydroxy polyethylene glycol acrylate, N-vinyl pyrrolidone, absolute ethyl alcohol and deionized water into a reaction bottle, heating to 60 ℃ under the protection of nitrogen, adding azodiisobutyronitrile into the reaction bottle while stirring, keeping the weight ratio of the hydroxy polyethylene glycol acrylate to the N-vinyl pyrrolidone to the absolute ethyl alcohol to the deionized water to the azodiisobutyronitrile to be 8:19:5:2:0.001, carrying out heat preservation and stirring reaction for 6 hours to obtain a reaction solution, cooling the reaction solution to room temperature, centrifuging at 8000rpm for 5 minutes to obtain a solid, washing the solid with the absolute ethyl alcohol for 3 times, and carrying out vacuum drying at 40 ℃ to constant weight to obtain the auxiliary agent.

Example 2:

the seaweed was treated according to the following steps:

s1, cleaning seaweed raw materials, then air-drying, crushing by a crusher, sieving by a 50-mesh sieve to obtain seaweed powder, adding the seaweed powder into distilled water, heating to 75 ℃ and then steaming for 2 hours to obtain seaweed solution, wherein the weight ratio of the seaweed powder to the distilled water is 1:15;

s2, squeezing and filtering the seaweed solution obtained in the step S1 by using a filter press to obtain filtrate and filter residues, adding complex enzyme consisting of cellulase, pectase and algin lyase in a weight ratio of 2:2:1 into the filtrate, adjusting the pH value to 5, heating to 45 ℃ for enzymolysis for 10 hours to obtain enzymolysis liquid, and centrifuging the enzymolysis liquid at 5000rpm for 5 minutes to obtain supernatant;

s4, concentrating the desalted liquid obtained in the step S3 in vacuum at 60 ℃ and 0.09Mpa vacuum degree for 2 hours to obtain a concentrate, and drying the concentrate at 30 ℃ for 2 hours to obtain the seaweed extract;

s5, drying the filter residue obtained in the step S2 at 50 ℃ for 3 hours to obtain seaweed residues, mixing the seaweed residues, the enzymolysis squid paste, the marine fish protein peptide, the auxiliary agent and the deionized water, and stirring until the seaweed residues, the enzymolysis squid paste, the marine fish protein peptide, the auxiliary agent and the deionized water are uniformly mixed to obtain a mixture, wherein the seaweed residues are 5 parts, the enzymolysis squid paste is 6 parts, the marine fish protein peptide is 2 parts, the auxiliary agent is 0.5 part and the deionized water is 30 parts by weight;

s6, drying the mixture obtained in the step S5 for 3 hours at 50 ℃ by using a rotary grinding drying tower, crushing by using a crusher, and sieving by using a 50-mesh sieve to obtain the compound seaweed powder.

The preparation procedure of the auxiliary agent in step S5 is the same as in example 1.

Example 3:

the seaweed was treated according to the following steps:

s1, cleaning seaweed raw materials, then air-drying, crushing by a crusher, sieving by a 50-mesh sieve to obtain seaweed powder, adding the seaweed powder into distilled water, heating to 75 ℃ and then steaming for 3 hours to obtain a seaweed solution, wherein the weight ratio of the seaweed powder to the distilled water is 1:15;

s2, squeezing and filtering the seaweed solution obtained in the step S1 by using a filter press to obtain filtrate and filter residues, adding complex enzyme consisting of cellulase, pectase and algin lyase in a weight ratio of 2:2:1 into the filtrate, adjusting the pH value to 5, heating to 45 ℃ for enzymolysis for 12 hours to obtain enzymolysis liquid, and centrifuging the enzymolysis liquid at 5000rpm for 10 minutes to obtain supernatant;

s4, concentrating the desalted liquid obtained in the step S3 in vacuum at 60 ℃ and 0.09Mpa vacuum degree for 1 hour to obtain a concentrate, and drying the concentrate at 30 ℃ for 3 hours to obtain the seaweed extract;

s5, drying the filter residue obtained in the step S2 at 50 ℃ for 2 hours to obtain seaweed residues, mixing the seaweed residues, the enzymolysis squid paste, the marine fish protein peptide, the auxiliary agent and the deionized water, and stirring until the seaweed residues, the enzymolysis squid paste, the marine fish protein peptide, the auxiliary agent and the deionized water are uniformly mixed to obtain a mixture, wherein the seaweed residues are 10 parts, the enzymolysis squid paste is 3 parts, the marine fish protein peptide is 5 parts, the auxiliary agent is 0.5 part and the deionized water is 20 parts by weight;

s6, drying the mixture obtained in the step S5 for 4 hours at 50 ℃ by using a rotary grinding drying tower, crushing by using a crusher, and sieving by using a 50-mesh sieve to obtain the compound seaweed powder.

Example 4:

the seaweed was treated according to the following steps:

s2, squeezing and filtering the seaweed solution obtained in the step S1 by using a filter press to obtain filtrate and filter residues, adding complex enzyme consisting of cellulase, pectase and algin lyase in a weight ratio of 2:2:1 into the filtrate, adjusting the pH value to 5, heating to 45 ℃ and performing enzymolysis for 10.5 hours to obtain enzymolysis solution, and centrifuging the enzymolysis solution at a rotation speed of 5000rpm for 6 minutes to obtain supernatant;

s4, concentrating the desalted liquid obtained in the step S3 in vacuum at 60 ℃ and 0.09Mpa vacuum degree for 1.5 hours to obtain a concentrate, and drying the concentrate at 30 ℃ for 2 hours to obtain the seaweed extract;

s5, drying the filter residue obtained in the step S2 at 50 ℃ for 2 hours to obtain seaweed residues, mixing the seaweed residues, the enzymolysis squid paste, the marine fish protein peptide, the auxiliary agent and the deionized water, and stirring until the seaweed residues, the enzymolysis squid paste, the marine fish protein peptide, the auxiliary agent and the deionized water are uniformly mixed to obtain a mixture, wherein the seaweed residues are 6 parts, the enzymolysis squid paste is 5 parts, the marine fish protein peptide is 4 parts, the auxiliary agent is 0.5 part and the deionized water is 27 parts;

Reference example 1:

the difference from example 1 is that no auxiliary agent was added in step S5, and the other steps are the same as in example 1.

Test example:

the compound seaweed powder prepared in example 1 and reference example 1 (seaweed raw materials used in example 1 and reference example 1 are gulfweed) is added into fish feed, and the fish feed comprises the following components in parts by weight: 40 parts of fish meal, 15 parts of compound seaweed meal, 15 parts of soybean meal, 20 parts of flour, 1 part of multi-vitamin multi-mineral premix, 0.4 part of choline chloride, 1.5 parts of calcium hydrophosphate, 0.1 part of vitamin C, 2 parts of fish oil and 5 parts of soybean oil.

Healthy and undamaged juvenile groupers (initial individual body weight is 31.5-31.6 g) are randomly selected and divided into three test groups, namely a first test group, a second test group and a third test group, wherein the first test group is added with the fish feed of the embodiment 1, the second test group is added with the fish feed of the embodiment 2, the fish feed used in the third test group is different from the first test group in that no compound seaweed meal is added, and the weight part of fish meal is 55 parts. Young groupers are put in a glass jar cultivation barrel, 3 repeats are arranged in each test group, 30 fish are arranged in each repeat, and the cultivation time period is 8 weeks. The fish feed is fed for 1 time at 8 points and 16 points each day, the temperature of the water body is 28-30 ℃ during the test, the dissolved oxygen is more than or equal to 5mg/L, the ammonia nitrogen content is less than or equal to 0.035mg/L, and the water exchange amount is about 70% each day. After the 8-week test is finished, weighing is carried out after feeding is forbidden for 24 hours, and the weight gain rate and the survival rate of each group are respectively calculated:

weight gain = (last average weight-first average weight)/first average weight x 100%;

survival = viable fish tail at the end of the trial/viable fish tail at the beginning of the trial x 100%.

The test results are shown in the following table:

	weight gain Rate (%)	Survival (%)
			Test group one	257.39±2.45	100
Test group two	246.53±3.20	100
			Test group three	232.66±1.87	100

As can be seen from the table, the weight gain rates of the first test group and the second test group are higher than that of the third test group, and the weight gain rate of the first test group is higher than that of the second test group, so that the auxiliary agent prepared by the invention can effectively improve the growth promoting effect of the compound seaweed powder on the cultured animals.

The above embodiments are merely illustrative of the principles of the present invention and its effectiveness, and are not intended to limit the invention. Modifications and variations may be made to the above-described embodiments by those skilled in the art without departing from the spirit and scope of the invention. Accordingly, it is intended that all equivalent modifications and variations of the invention be covered by the claims, which are within the ordinary skill of the art, be within the spirit and scope of the present disclosure.

Claims

1. A seaweed treatment method is characterized in that: the method comprises the following steps:

2. A method of processing seaweed as claimed in claim 1, characterized in that: in the step S1, a 50-mesh sieve is used for sieving, and the weight ratio of the seaweed powder to the distilled water is 1:15.

3. A method of processing seaweed as claimed in claim 1, characterized in that: in the step S2, the complex enzyme consists of cellulase, pectase and algin lyase in a weight ratio of 2:2:1, wherein the weight ratio of the complex enzyme to the filtrate is 1:125, and the rotating speed during centrifugation is 5000rpm.

4. A method of processing seaweed as claimed in claim 1, characterized in that: in the step S3, the molecular weight cut-off of the ultrafiltration membrane is 1000Da, the operation voltage during electrodialysis desalination is 30V, and the loading flow rate is 50L/hour.

5. A method of processing seaweed as claimed in claim 1, characterized in that: in the step S4, the temperature during vacuum concentration is 60 ℃, and the vacuum degree is 0.09Mpa; the temperature at the time of low-temperature drying was 30 ℃.

6. A method of processing seaweed as claimed in claim 1, characterized in that: in the step S5, the temperature at the time of drying is 50 ℃; according to the weight portions, 5 to 10 portions of seaweed residues, 3 to 6 portions of enzymolysis squid paste, 2 to 5 portions of marine fish protein peptide, 0.5 portion of auxiliary agent and 20 to 30 portions of deionized water.

7. A method for processing seaweed as claimed in claim 6, characterized in that: in the step S5, the auxiliary agent is prepared from the following steps:

8. A method for processing seaweed as claimed in claim 7, characterized in that: in the preparation step of the auxiliary agent, the weight ratio of the hydroxyl polyethylene glycol acrylate to the N-vinyl pyrrolidone to the absolute ethyl alcohol to the deionized water to the azodiisobutyronitrile is 8:19:5:2:0.001, the rotating speed during centrifugation is 8000rpm, and the temperature during vacuum drying is 40 ℃.

9. A method of processing seaweed as claimed in claim 1, characterized in that: in the step S6, the drying temperature of the rotary grinding drying tower is 50 ℃, and a 50-mesh sieve is used for sieving.