CN116530261A - Breeding method for comprehensively preventing and controlling pineapple hybrid seedling disease - Google Patents
Breeding method for comprehensively preventing and controlling pineapple hybrid seedling disease Download PDFInfo
- Publication number
- CN116530261A CN116530261A CN202310460956.9A CN202310460956A CN116530261A CN 116530261 A CN116530261 A CN 116530261A CN 202310460956 A CN202310460956 A CN 202310460956A CN 116530261 A CN116530261 A CN 116530261A
- Authority
- CN
- China
- Prior art keywords
- seeds
- seedling
- pineapple
- seedlings
- sponge
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 235000007119 Ananas comosus Nutrition 0.000 title claims abstract description 58
- 238000009395 breeding Methods 0.000 title claims abstract description 46
- 201000010099 disease Diseases 0.000 title claims abstract description 31
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 title claims abstract description 31
- 244000099147 Ananas comosus Species 0.000 title abstract description 3
- 230000001954 sterilising effect Effects 0.000 claims abstract description 50
- 235000013399 edible fruits Nutrition 0.000 claims abstract description 45
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 36
- 230000035784 germination Effects 0.000 claims abstract description 34
- 230000001488 breeding effect Effects 0.000 claims abstract description 30
- 238000011282 treatment Methods 0.000 claims abstract description 16
- 230000002159 abnormal effect Effects 0.000 claims abstract description 13
- 238000003306 harvesting Methods 0.000 claims abstract description 12
- 238000002791 soaking Methods 0.000 claims abstract description 12
- 238000005406 washing Methods 0.000 claims abstract description 8
- 239000011159 matrix material Substances 0.000 claims abstract description 5
- 241000234671 Ananas Species 0.000 claims description 55
- 241000243142 Porifera Species 0.000 claims description 49
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 39
- 239000007788 liquid Substances 0.000 claims description 32
- 241000196324 Embryophyta Species 0.000 claims description 20
- 238000000034 method Methods 0.000 claims description 19
- 239000008223 sterile water Substances 0.000 claims description 19
- 235000015097 nutrients Nutrition 0.000 claims description 16
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 14
- 230000008569 process Effects 0.000 claims description 11
- 238000012423 maintenance Methods 0.000 claims description 10
- 239000004563 wettable powder Substances 0.000 claims description 10
- 230000001133 acceleration Effects 0.000 claims description 9
- 230000007480 spreading Effects 0.000 claims description 9
- 238000004140 cleaning Methods 0.000 claims description 8
- 239000003814 drug Substances 0.000 claims description 8
- 208000015181 infectious disease Diseases 0.000 claims description 8
- 230000036541 health Effects 0.000 claims description 7
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 claims description 6
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 claims description 6
- 239000006013 carbendazim Substances 0.000 claims description 6
- 238000013461 design Methods 0.000 claims description 6
- 238000009396 hybridization Methods 0.000 claims description 6
- 238000005286 illumination Methods 0.000 claims description 5
- 230000010152 pollination Effects 0.000 claims description 5
- 239000005802 Mancozeb Substances 0.000 claims description 4
- 229910004616 Na2MoO4.2H2 O Inorganic materials 0.000 claims description 4
- 239000005842 Thiophanate-methyl Substances 0.000 claims description 4
- 239000011248 coating agent Substances 0.000 claims description 4
- 238000000576 coating method Methods 0.000 claims description 4
- 230000002354 daily effect Effects 0.000 claims description 4
- 239000012153 distilled water Substances 0.000 claims description 4
- 230000003203 everyday effect Effects 0.000 claims description 4
- 239000002689 soil Substances 0.000 claims description 4
- 229910001220 stainless steel Inorganic materials 0.000 claims description 4
- 239000010935 stainless steel Substances 0.000 claims description 4
- QGHREAKMXXNCOA-UHFFFAOYSA-N thiophanate-methyl Chemical group COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC QGHREAKMXXNCOA-UHFFFAOYSA-N 0.000 claims description 4
- 230000009286 beneficial effect Effects 0.000 claims description 3
- 238000005507 spraying Methods 0.000 claims description 3
- 230000001502 supplementing effect Effects 0.000 claims description 3
- 208000035143 Bacterial infection Diseases 0.000 claims description 2
- 208000022362 bacterial infectious disease Diseases 0.000 claims description 2
- 238000012258 culturing Methods 0.000 claims description 2
- 238000003973 irrigation Methods 0.000 claims description 2
- 230000002262 irrigation Effects 0.000 claims description 2
- 238000004804 winding Methods 0.000 claims description 2
- 230000001276 controlling effect Effects 0.000 abstract 3
- 230000001105 regulatory effect Effects 0.000 abstract 1
- 238000010586 diagram Methods 0.000 description 6
- 230000007226 seed germination Effects 0.000 description 4
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 238000002513 implantation Methods 0.000 description 3
- 230000004083 survival effect Effects 0.000 description 3
- 206010058314 Dysplasia Diseases 0.000 description 2
- 238000009402 cross-breeding Methods 0.000 description 2
- 230000006806 disease prevention Effects 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 229930091371 Fructose Natural products 0.000 description 1
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 1
- 239000005715 Fructose Substances 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 229930003270 Vitamin B Natural products 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 239000010000 changlu Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 235000012054 meals Nutrition 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
- 235000019156 vitamin B Nutrition 0.000 description 1
- 239000011720 vitamin B Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/02—Germinating apparatus; Determining germination capacity of seeds or the like
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/05—Fruit crops, e.g. strawberries, tomatoes or cucumbers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Soil Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Physiology (AREA)
- Botany (AREA)
- Pest Control & Pesticides (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention discloses a breeding method for comprehensively preventing and controlling pineapple hybrid seedling diseases, which comprises the following steps: (1) harvesting hybrid fruits and seeds at proper time; (2) washing and sorting seeds; (3) seed soaking sterilization; (4) maintaining the sterile operation of the breeding environment; (5) selecting a breathable water-retaining breeding matrix for germination accelerating and seedling raising; (6) preventing and controlling seedling diseases; (7) removing abnormal seedlings in time; (8) transplanting the plug tray at the right time. The invention can reduce seedling disease by regulating and controlling seed harvesting time, seed disinfection treatment, seedling disease control and other measures, so that the germination rate of seeds can reach more than 90% and the emergence rate can reach more than 80%.
Description
Technical Field
The invention belongs to the technical field of crop breeding, and particularly relates to a breeding method for comprehensively preventing and controlling pineapple hybrid seedling diseases.
Background
Pineapple (Ananas comosus (L.) Merr.) is also known as pineapple, belongs to the genus pineapple of the family pineapple, and is one of the best known tropical fruits, namely, a perennial monocotyledonous She Changlu herb. Pineapple fruits are rich in nutrition, contain a large amount of fructose, glucose, vitamin B, C, phosphorus, citric acid, protease and other substances, and are one of the fruits with the highest global economic value.
At present, the good pineapple cultivated worldwide has few varieties, so that the existing germplasm resources are urgently excavated, and the hybridization breeding work is greatly carried out by utilizing specific characters. Because pineapple seeds are smaller, a common method adopts a method of filling filter paper in a culture dish, and a new and improved method is a patent filed earlier by the applicant, and the name is: a pineapple seed ventilation and water retention breeding method, patent number: ZL202210193482.1 is cultivated by filling sponge in a culture dish. The pineapple seeds are placed on filter paper or sponge to spray water, culture conditions are controlled, and breeding is carried out under proper illumination and temperature. However, the existing pineapple seed breeding technology has a plurality of disadvantages: researches show that the early pineapple fruits and seeds are collected and the seedling breeding link is not properly treated, so that the seedlings are easy to infect and spread diseases in a high-temperature and high-humidity environment of a climatic chamber, the survival rate of the seedlings is reduced or is very low, and finally the seedlings are completely abandoned before the pollination cross breeding work of the pineapples. Therefore, how to make the whole link seedling raising work of pineapple seeds, and disease prevention and control are key steps for improving the breeding work and efficiency.
Disclosure of Invention
The invention aims to provide a breeding method for comprehensively preventing and controlling seedling diseases of pineapple hybrid seedlings, which is characterized by taking measures such as early-stage seed acquisition, seed cultivation and disinfection, seedling disease control and the like, so that seedling disease conditions are reduced and lowered.
In order to achieve the above purpose, the present invention provides the following scheme:
the breeding method for comprehensively preventing and controlling the seedling disease of pineapple hybrid seedlings comprises the following steps:
(1) Harvesting the hybrid fruits and seeds at proper time: the harvesting is arranged when the color of the bottom surface of the fruit is changed by 1/3-2/3 after pollination and hybridization, and the harvesting is carried out in batches according to the maturity of pineapple, so that the problem that a large number of saprophytes are bred in seeds due to excessive ripeness and rot of the pineapple and the subsequent seedling breeding work is directly influenced is avoided. Meanwhile, the diseased fruits and rotten fruits are removed, the seeds with bacterial infection are prevented from being collected, the seeds in the fruit meshes are picked safely and nondestructively by using an integrated dual-purpose fork and spoon after the fruits are peeled, the mechanical damage of the seeds is reduced as much as possible, and the seeds can be prevented from being infected and moldy due to the damage of scratch and wound in the germination accelerating process.
(2) Cleaning and sorting seeds: the collected fresh seeds are cleaned in time, so that the subsequent seedling breeding work is not directly affected by a large amount of saprophyte which is smelly and smelly due to untimely cleaning of the seeds. Washing with clear water, rinsing pulp and empty and shrunken seeds, draining, wrapping the seeds with a dry towel, repeatedly lightly rubbing to thoroughly wipe the transparent seed coating attached to the seed coat, carefully picking again to remove the empty and shrunken seeds and mechanical damage, and draining for later use;
(3) Seed soaking sterilization: soaking the cleaned seeds in a sterilizing liquid to sterilize, and washing the seeds for later use after the sterilization is completed;
(4) The breeding environment keeps aseptic operation: the culture environment of the artificial climate box is set to have the illumination intensity of 5000Lx, the temperature of 27 ℃ and the humidity of 80%, 14-hour light culture and 10-hour dark culture, 75% alcohol is not regularly wiped inside and outside the climate box for disinfection treatment, an ultra-clean bench is required to be used in the seedling breeding process, an ultraviolet lamp is required to be started for disinfection 20-30 minutes in advance before the ultra-clean bench is used, 75% alcohol is sprayed to wipe the table top after the disinfection is finished, a fan is started, and the ultra-clean bench and a vessel are kept clean through the whole-course disinfection in the use process;
(5) Selecting a breathable water-retaining breeding matrix for germination acceleration and seedling culture: spreading breathable water-retaining sponge in a culture dish A, adding sterile water, spreading treated pineapple seeds on the water-absorbing sponge, placing the pineapple seeds in a climatic chamber for germination acceleration, and taking care of supplementing the sterile water; after accelerating germination for 5 days, taking care of each day to observe the germination condition of the seeds; timely picking out the broken germinated seeds and placing the seeds into a new sponge culture dish B; after that, sterile water is supplemented once every 15-20 days according to the dry and wet condition of the sponge;
(6) Preventing and controlling seedling diseases: seedling breeding is carried out in a climatic chamber, seedlings which are infected with heart rot and black rot are removed by tweezers in time, the seedlings which are not infected with the diseases in the same culture dish are prevented and controlled by applying 70% mancozeb wettable powder 800 times of medicine drops to the irrigation cores, and the medicine application is repeated once every 10-15 days; the used forceps are sterilized in time;
(7) Removing malformed seedlings in time: abnormal seedlings cannot grow and develop normally and are extremely easy to die and rot early, tweezers are used for removing the abnormal seedlings cleanly, sterilizing liquid is added into the original abnormal seedling mildew areas for sterilization treatment, sterilizing liquid is added into the abnormal seedlings according to the growth health conditions of the seedlings for maintenance, and the used tweezers are sterilized in time;
(8) Transplanting the plug tray at proper time: transplanting when the seedling age of the seedling exceeds 40 days and the plant grows 5-7 mature leaves, cutting the seedling together with the sponge at the base of the plant into a plant of sponge root-protecting seedling by using scissors, then planting the plant in a cave dish filled with nutrient soil, slowly spraying root-fixing water until the bottom of the cave dish is slightly permeable, and finally placing the plant on a seedling bed for daily maintenance.
As the scheme is preferable, the 3-fork-tooth blunt tip design of the integrated dual-purpose fork spoon is slender and round, the 3-fork-tooth blunt tip is not beneficial, mechanical damage caused when seeds are harvested is avoided, and the fork spoon is 15.8cm long and 2.1cm wide, so that the seeds in the fruit-eye cavity can be conveniently manually dug.
Preferably, the sterilization liquid in the step (3) is used for soaking seeds for 16 to 20 hours.
As the scheme is preferable, the breathable water-retaining sponge is flatly paved in the culture dish A in the step (5), and clean stainless steel ordinary meal spoon is used for extruding the sponge while adding sterile water into the culture dish so as to enable the sponge to uniformly and fully absorb water, so that good growth and development microenvironment and space can be provided for seed germination and seedling implantation growth. Uniformly spreading the cleaned and sterilized pineapple seeds on a water-absorbing sponge, covering a culture dish, marking, placing the pineapple seeds in a climatic chamber for germination, timely adding sterile water according to the dry and wet conditions of the sponge for 7-10 days, and observing the germination condition of the pineapple seeds every day after 5 days of germination; timely picking out the broken germinated seeds, placing the broken germinated seeds into a new sponge culture dish B, and culturing the broken germinated seeds and the ungerminated seeds separately for seedling culture so as to prevent the root systems of the germinated seedlings from winding, covering and preventing the ungerminated seeds from developing.
As a preferable scheme, in the step (5), 1ml to 2ml of improved Hoagland nutrient solution is added for 1 time while the sponge culture dish B is supplemented with sterile water, wherein the improved Hoagland nutrient solution comprises the following components: ca (NO) 3 ) 2 .4H 2 O 900-950mg/L、KNO 3 500-520mg/L、NH 4 NO 3 80-85mg/L、KH 2 PO 4 130-140mg/L、MgSO 4 240-245mg/L、FeNaEDTA 36-37mg/L、KI 0.8-1.0mg/L、H 3 BO 3 6.0-6.5mg/L、MnSO 4 .H 2 O 16.5-17.0mg/L、ZnSO 4 .7H 2 O 8.5-9.0mg/L、Na 2 MoO 4 .2H 2 O 0.25mg/L、CuSO 4 .5H 2 O 0.025-0.030mg/L、CoCl 2 .6 H 2 O0.025-0.030mg/L, ph=5.8; when in use, the improved Hoagland nutrient solution is diluted by 100 times with distilled water.
Preferably, the tweezers used in the steps (6) and (7) avoid contacting with breeding matrixes and other healthy seedlings, so that new infection is caused, and the tweezers are thoroughly sterilized by burning with an alcohol lamp after each use.
In the step (7), 2-3ml of sterilizing liquid is added to the mildewed area of the original malformed seedling for treatment, and 1-2ml of sterilizing liquid is added according to the growth health condition of the seedling for maintenance.
As the scheme, the sterilizing liquid adopted by the invention is 500 times of 50% carbendazim wettable powder or 70% thiophanate methyl 500 times of sterilizing liquid, and the sterilizing liquid is used alternately.
The sponge adopted in the invention is white 5mm high-density sponge.
The invention has the following beneficial effects:
1. by the comprehensive prevention and control method, the risk of pineapple cross breeding can be effectively avoided, seedlings germinate neatly and rapidly, germination of seeds can be completed after 7-25 days, and the germination rate of the seeds reaches more than 90%.
2. The seedlings cultivated by the method are strong in growth, the emergence rate is more than 80%, and the front-back breeding period can be shortened by about one month.
3. The method is simple and convenient, can obviously improve the seedling emergence rate, shortens the hybridization time, is easy to obtain large-scale high-quality healthy hybrid seedlings, and is easy to cultivate excellent and innovative germplasm.
Drawings
FIG. 1 is a diagram of the operation of taking pineapple seeds by an integrated dual-purpose fork and spoon.
Fig. 2 is a plot of pineapple seed sorting out and sorting records.
FIG. 3 is a working diagram of the super clean bench pineapple breeding seedlings.
FIG. 4 is a diagram showing the serious disease of germinated white seeds caused by infection of heart rot by germinated seeds.
FIG. 5 is a graph showing the occurrence of severe death of seedlings caused by infection of seedlings with heart rot.
FIG. 6 is a diagram of malformed seedlings showing dysplasia during seed seedling raising.
Fig. 7 is a graph of malformed seedlings with dysplasia removed in time.
FIG. 8 is a diagram of a robust seedling of pineapple bred by the method of the invention.
Fig. 9 is a diagram showing the transplanting condition of pineapple seedlings.
Detailed Description
The invention is further described below with reference to examples:
example 1
The influence of harvesting of different fruit maturity on germination conditions of the seed germination acceleration is as follows:
(1) Harvesting hybrid fruits and seeds: fruits are harvested according to different maturity for 3-4 months after pollination hybridization, and 10 fruits are harvested in each group.
Group A: 1/3 of the fruits are colored, and the ill fruits and rotten fruits are removed; group B: 2/3 of the fruits are changed in color, and the ill fruits and rotten fruits are removed; group C: overripe fruits and no defective fruits and rotten fruits are removed (seed-carrying bacteria are easy to cause incomplete seed soaking sterilization, so that the occurrence of germination accelerating seeds in FIG. 4 causes the occurrence of germination dew white seeds due to heart rot infection);
and randomly screening 3 healthy and full fruits from 10 fruits in each group, peeling the fruits, and using an integrated dual-purpose fork and spoon to safely and nondestructively dig seeds in the order of fruits (shown in figure 1). The design of the 3-fork tines which are slender and round is a whole dual-purpose fork and spoon, and the design is sharp and unfavorable, so that mechanical damage caused when harvesting seeds is avoided, and the fork and spoon is 15.8cm long and 2.1cm wide, so that the seeds in the fruit-eye cavities can be conveniently manually excavated.
(2) Cleaning and sorting seeds: and cleaning the collected fresh seeds in time. Washing with clear water, rinsing pulp and empty and shrunken seeds, draining, wrapping the seeds with a dry towel, repeatedly lightly rubbing to thoroughly wipe the transparent seed coating attached to the seed coat, carefully picking again to remove the empty and shrunken seeds and mechanical damage, washing with water, and draining; as shown in fig. 2, the sorted high quality pineapple seeds are placed in seed collection bottles, each fruit is provided with 1 collection bottle, and marked for later use.
(3) Seed soaking sterilization: and randomly selecting 100 healthy and full seeds from each collecting bottle, soaking and sterilizing, putting the seeds into 500 times of 50% carbendazim wettable powder for 16 hours, and cleaning after sterilization.
(4) The breeding environment keeps aseptic operation: before seed cultivation is started, 75% alcohol is irregularly wiped inside and outside a climatic box for disinfection treatment, an ultra-clean workbench is required to be used in a standard manner in the seedling breeding process, an ultraviolet lamp is required to be started to kill in advance for 20-30 minutes before the ultra-clean workbench is used, 75% alcohol is sprayed to wipe a table top after the disinfection is finished, a fan is started, and the ultra-clean workbench and a vessel are kept clean through the whole process of disinfection in the use process; as shown in fig. 3. The culture environment of the artificial climate box is set to be 5000Lx in illumination intensity, 27 ℃ in temperature and 80% in humidity, 14-hour light culture and 10-hour dark culture.
(5) Selecting a breathable water-retaining breeding matrix for germination acceleration and seedling culture: a white high-density sponge with the thickness of 5mm is paved in a culture dish, sterile water is added into the culture dish, and a clean stainless steel common spoon is used for extruding the sponge to enable the sponge to uniformly and fully absorb water, so that good micro-environments and spaces for seed germination and seedling implantation growth can be provided. And uniformly spreading the pineapple seeds selected from the A, B, C group on different sponges, covering a culture dish, marking, placing in a climatic chamber for germination acceleration, and adding sterile water timely according to the dry and wet conditions of the sponges for 7-10 days. Meanwhile, mildewed seeds were recorded and counted every 5 days (as shown in fig. 4 to 5) and picked out and recorded in table 1. After germination accelerating for 5 days, the germination condition of the seeds needs to be observed with care every day; timely picking out the broken germinated seeds and placing the seeds into a new sponge culture dish.
TABLE 1 Table 1 infection of different fruit maturity on seedling germination diseases
As shown in Table 1, the pineapple is harvested at the time of 1/3-2/3 color conversion, so that the mildew and rot number of seeds can be effectively reduced. And the fruits are overripe, and the pathogenic bacteria are easily caused when the fruits are not removed, so that the probability of the mildewing of the seeds is increased.
Example 2
The pineapple seedling disease prevention and control treatment influence experiment comprises the following specific operation steps:
(1) And (3) setting 3 repetitions for each experimental group and control group, and randomly selecting 30 healthy pineapple seedlings for each sample.
The pineapple seedling culture dish is supplemented with sterile water every 15-20 days according to the dry and wet condition of the sponge; meanwhile, 1ml of improved Hoagland nutrient solution is added for 1 time, and the improved Hoagland nutrient solution comprises the following components: ca (NO) 3 ) 2 .4H 2 O 900-950mg/L、KNO 3
500-520mg/L、NH 4 NO 3 80-85mg/L、KH 2 PO 4 130-140mg/L、MgSO 4 240-245mg/L、FeNaEDTA 36-37mg/L、KI 0.8-1.0mg/L、H 3 BO 3 6.0-6.5mg/L、MnSO 4 .H 2 O 16.5-17.0mg/L、ZnSO 4 .7H 2 O8.5-9.0mg/L、Na 2 MoO 4 .2H 2 O 0.25mg/L、CuSO 4 .5H 2 O 0.025-0.030mg/L、CoCl 2 .6 H 2 O0.025-0.030mg/L, ph=5.8; when in use, the improved Hoagland nutrient solution is diluted by 100 times with distilled water.
(2) Preventing and controlling seedling diseases: seedling breeding is carried out in a manual climatic chamber, and seedlings which are found to be infected with heart rot and black rot in the culture dish B are removed by tweezers in time, as shown in fig. 6 and 7. The used forceps are thoroughly burned by an alcohol lamp and sterilized in time.
Experimental group: the method comprises the steps of (1) preventing and controlling the seedlings which are not infected in the same culture dish by adopting 800 times of drug drops of 70% mancozeb wettable powder to apply and irrigate cores, and repeatedly applying the drugs once at intervals of 10-15 days;
control group: sterile water was added dropwise to the uninfected seedlings in the same dish.
(7) Removing malformed seedlings in time: abnormal seedlings cannot grow and develop normally and are very easy to die and rot early, tweezers are used for removing the abnormal seedlings, and used tweezers are thoroughly and timely sterilized by being burned by an alcohol lamp (shown in fig. 7).
Experimental group: 2-3ml of sterilizing liquid is added into the original deformed seedling mildew area for sterilization treatment, 1-2ml of sterilizing liquid is added according to the growth health condition of the seedling for maintenance, the sterilizing liquid is 500 times of 50% carbendazim wettable powder or 70% thiophanate methyl 500 times of sterilizing liquid, and the sterilizing liquid is used alternately.
Control group: the original malformed seedling moldy area is not treated.
(8) Transplanting the plug tray at proper time: transplanting when the seedling age of the seedling exceeds 40 days and the plant grows 5-7 mature leaves, cutting the seedling together with the sponge at the base of the plant into a plant of sponge root-protecting seedling by using scissors, then planting the plant in a cave dish filled with nutrient soil, slowly spraying root-fixing water until the bottom of the cave dish is slightly permeable, and finally placing the plant on a seedling bed for daily maintenance.
TABLE 2 Effect of disease control treatments on pineapple seedling growth
In FIG. 7, malformed Miao Tu is removed in time, 7 out of 9 malformed seedlings are removed from 1 dish for about 25 days, mildew and rot are generated, and then 2-3ml of sterilizing liquid is added to the mildew area of the original malformed seedlings for sterilization treatment according to the treatment of an experimental group, and 1-2ml of sterilizing liquid is added for curing according to the health condition of the seedlings, so that the occurrence of seedling diseases can be effectively prevented and controlled; if the control group is 'the original malformed seedling mould-forming area is not treated', the serious infection and morbidity phenomenon is most likely to occur when the seedlings grow in a warm and moist climatic box environment, and the serious seedling death phenomenon is likely to occur when the seedlings are infected with heart rot in figure 5.
From the analysis of pictures and the results shown in Table 2, the invention adopts the core filling and the sterilization and disinfection treatment of the sterilization liquid, can effectively delay and reduce the mildew and rot conditions of seedlings, and can achieve the seedling rate of more than 93.3 percent. In the control group, the condition of mildew and rot of seedlings is aggravated due to pathogen infection, and the seedling rate is reduced.
Example 3
The breeding method for comprehensively preventing and controlling the seedling disease of pineapple hybrid seedlings comprises the following steps:
(1) Harvesting hybrid fruits and seeds: harvesting is carried out 3-4 months after pollination and hybridization, and 1/3-2/3 of the color of the bottom surface of the fruit is changed, and the ill fruit and rotten fruit are removed. The seeds in the fruit meshes are picked up safely and atraumatically by using an integrated dual-purpose fork and spoon after the fruits are peeled. The design of the 3-fork tines which are slender and round is a whole dual-purpose fork and spoon, and the design is sharp and unfavorable, so that mechanical damage caused when harvesting seeds is avoided, and the fork and spoon is 15.8cm long and 2.1cm wide, so that the seeds in the fruit-eye cavities can be conveniently manually excavated.
(2) Cleaning and sorting seeds: and cleaning the collected fresh seeds in time. Washing with clear water, rinsing pulp and empty and shrunken seeds, draining, wrapping the seeds with a dry towel, repeatedly lightly rubbing to thoroughly wipe the transparent seed coating attached to the seed coat, carefully picking and removing the empty and shrunken seeds, washing with water, and draining. The sorted high-quality pineapple seeds are put into a seed collecting bottle and marked for standby.
(3) Seed soaking sterilization: healthy and full seeds are selected from each collecting bottle for seed soaking sterilization, the seeds are placed into 500 times of 50% carbendazim wettable powder for 16 hours, and the sterilization is completed and the seeds are washed clean.
(4) The breeding environment keeps aseptic operation: before seed cultivation is started, 75% alcohol is irregularly wiped inside and outside a climatic box for disinfection treatment, an ultra-clean workbench is required to be used in a standard manner in the seedling breeding process, an ultraviolet lamp is required to be started to kill in advance for 20-30 minutes before the ultra-clean workbench is used, 75% alcohol is sprayed to wipe a table top after the disinfection is finished, a fan is started, and the ultra-clean workbench and a vessel are kept clean through the whole process of disinfection in the use process; as shown in fig. 3. The culture environment of the artificial climate box is set to be 5000Lx in illumination intensity, 27 ℃ in temperature and 80% in humidity, 14-hour light culture and 10-hour dark culture.
(5) Selecting a breathable water-retaining breeding matrix for germination acceleration and seedling culture: a white high-density sponge with the thickness of 5mm is paved in a culture dish, sterile water is added into the culture dish, and a clean stainless steel common spoon is used for extruding the sponge to enable the sponge to uniformly and fully absorb water, so that good micro-environments and spaces for seed germination and seedling implantation growth can be provided. And uniformly spreading the selected pineapple seeds on different sponges, covering a culture dish, marking, placing in a climatic chamber for germination acceleration, and adding sterile water timely according to the dry and wet conditions of the sponges for 7-10 days. If the seeds are mildewed, picking out the seeds in time. After germination accelerating for 5 days, the germination condition of the seeds needs to be observed with care every day; timely picking out the broken germinated seeds and placing the seeds into a new sponge culture dish.
(6) Timely picking out the seeds with the broken holes for germination, putting the seeds into a new sponge culture dish, making marks, and supplementing sterile water to the pineapple seedling culture dish every 15-20 days according to the dry and wet state of the sponge; meanwhile, 1ml of improved Hoagland nutrient solution is added for 1 time, and the improved Hoagland nutrient solution comprises the following components: ca (NO) 3 ) 2 .4H 2 O 900-950mg/L、KNO 3 500-520mg/L、NH 4 NO 3 80-85mg/L、KH 2 PO 4 130-140mg/L、MgSO 4 240-245mg/L、FeNaEDTA 36-37mg/L、KI
0.8-1.0mg/L、H 3 BO 3 6.0-6.5mg/L、MnSO 4 .H 2 O 16.5-17.0mg/L、ZnSO 4 .7H 2 O 8.5-9.0mg/L、Na 2 MoO 4 .2H 2 O 0.25mg/L、CuSO 4 .5H 2 O 0.025-0.030mg/L、CoCl 2 .6 H 2 O0.025-0.030mg/L, ph=5.8; when in use, the improved Hoagland nutrient solution is diluted by 100 times with distilled water.
(7) Preventing and controlling seedling diseases: seedling breeding is carried out in a manual climatic chamber, and the seedlings which are infected with the heart rot and the black rot are timely removed by forceps. The used forceps are thoroughly burned by an alcohol lamp and sterilized in time. The seedlings which are not infected in the same culture dish are prevented and controlled by adopting 70% mancozeb wettable powder and 800 times of medicine liquid drop applying cores, and the medicine application is repeated once again at intervals of 10-15 days.
(8) Removing malformed seedlings in time: abnormal seedlings cannot grow and develop normally and are very easy to die and rot early, tweezers are used for removing the abnormal seedlings cleanly, and the used tweezers are thoroughly and timely sterilized by being burned by an alcohol lamp. 2-3ml of sterilizing liquid is added into the original deformed seedling mildew area for sterilization treatment, 1-2ml of sterilizing liquid is added according to the growth health condition of the seedling for maintenance, the sterilizing liquid is 500 times of 50% carbendazim wettable powder or 70% thiophanate methyl 500 times of sterilizing liquid, and the sterilizing liquid is used alternately. The pineapple seedlings are shown in fig. 8.
(9) Transplanting the plug tray at proper time: transplanting after the seedling age exceeds 40 days and the plant grows 5-7 mature leaves, cutting the seedling together with the sponge at the base of the plant into a plant of sponge root-protecting seedling by using scissors (as shown in figure 9), then planting the plant in a cave dish filled with nutrient soil, taking the condition that water slowly drenches enough to fix the root until the bottom of the cave dish is slightly permeated, and finally placing the plant on a seedling bed for daily maintenance.
The experiment is carried out by using 500 seeds obtained by combining Tai-induced No. 8 and golden pineapple in a hybrid way, the germination rate of the seeds is 471, the germination period is 22 days, the strong seedlings 439 are cultivated, transplanting is carried out at proper time, the transplanting survival rate is 414, and the seedling transplanting survival rate is 82.8%.
While the foregoing describes the embodiments of the present invention, it should be understood that the present invention is not limited to the embodiments, and that various modifications and changes can be made by those skilled in the art without any inventive effort.
Claims (8)
1. The breeding method for comprehensively preventing and controlling the seedling disease of the pineapple hybrid seedlings is characterized by comprising the following steps of:
(1) Harvesting the hybrid fruits and seeds at proper time: collecting is arranged when the color of the bottom surface of the fruit is 1/3-2/3 of the color is changed after pollination hybridization, the fruits are collected in batches according to the maturity of pineapple, diseased fruits and rotten fruits are removed, the seeds with bacterial infection are prevented from being collected, and the seeds in the fruit meshes are safely and nondestructively dug by using an integrated two-purpose fork and spoon after the fruits are peeled;
(2) Cleaning and sorting seeds: the picked fresh seeds are cleaned in time, pulp and empty seeds are rinsed and rinsed by clear water, after draining, the seeds are wrapped by dry towels and repeatedly rubbed lightly, the transparent seed coating attached to the seed coats is thoroughly wiped clean, the empty and mechanical damaged seeds are carefully picked and removed again, and after water and draining, the seeds are reserved;
(3) Seed soaking sterilization: soaking the cleaned seeds in a sterilizing liquid to sterilize, and washing the seeds for later use after the sterilization is completed;
(4) The breeding environment keeps aseptic operation: the culture environment of the artificial climate box is set to have the illumination intensity of 5000Lx, the temperature of 27 ℃ and the humidity of 80%, 14-hour light culture and 10-hour dark culture, 75% alcohol is not regularly wiped inside and outside the climate box for disinfection treatment, an ultra-clean bench is required to be used in the seedling breeding process, an ultraviolet lamp is required to be started for disinfection 20-30 minutes in advance before the ultra-clean bench is used, 75% alcohol is sprayed to wipe the table top after the disinfection is finished, a fan is started, and the ultra-clean bench and a vessel are kept clean through the whole-course disinfection in the use process;
(5) Selecting a breathable water-retaining breeding matrix for germination acceleration and seedling culture: spreading breathable water-retaining sponge in a culture dish A, adding sterile water, spreading treated pineapple seeds on the water-absorbing sponge, placing the pineapple seeds in a climatic chamber for germination acceleration, and taking care of supplementing the sterile water; after accelerating germination for 5 days, taking care of each day to observe the germination condition of the seeds; timely picking out the broken germinated seeds and placing the seeds into a new sponge culture dish B; after that, sterile water is supplemented once every 15-20 days according to the dry and wet condition of the sponge;
(6) Preventing and controlling seedling diseases: seedling breeding is carried out in a climatic chamber, seedlings which are infected with heart rot and black rot are removed by tweezers in time, the seedlings which are not infected with the diseases in the same culture dish are prevented and controlled by applying 70% mancozeb wettable powder 800 times of medicine drops to the irrigation cores, and the medicine application is repeated once every 10-15 days; the used forceps are sterilized in time;
(7) Removing malformed seedlings in time: abnormal seedlings cannot grow and develop normally and are extremely easy to die and rot early, tweezers are used for removing the abnormal seedlings cleanly, sterilizing liquid is added into the original abnormal seedling mildew areas for sterilization treatment, sterilizing liquid is added into the abnormal seedlings according to the growth health conditions of the seedlings for maintenance, and the used tweezers are sterilized in time;
(8) Transplanting the plug tray at proper time: transplanting when the seedling age of the seedling exceeds 40 days and the plant grows 5-7 mature leaves, cutting the seedling together with the sponge at the base of the plant into a plant of sponge root-protecting seedling by using scissors, then planting the plant in a cave dish filled with nutrient soil, slowly spraying root-fixing water until the bottom of the cave dish is slightly permeable, and finally placing the plant on a seedling bed for daily maintenance.
2. The breeding method for comprehensively preventing and controlling seedling diseases of pineapple hybrid seedlings according to claim 1, which is characterized by comprising the following steps: the integrated dual-purpose fork and spoon is long and thin, round and smooth, 3-fork teeth are in blunt tip design, the tip is not beneficial, mechanical damage caused when seeds are harvested is avoided, and the fork and spoon is 15.8cm long and 2.1cm wide, so that the seeds in the fruit-eye cavities can be conveniently manually dug.
3. The breeding method for comprehensively preventing and controlling seedling diseases of pineapple hybrid seedlings according to claim 1 or 2, which is characterized by comprising the following steps: and (3) soaking seeds in the sterilizing liquid for 16-20 hours.
4. The breeding method for comprehensively preventing and controlling seedling diseases of pineapple hybrid seedlings according to claim 1 or 2, which is characterized by comprising the following steps: spreading the breathable water-retaining sponge in a culture dish A, adding sterile water into the culture dish while extruding the sponge by using a clean stainless steel common spoon to enable the sponge to be balanced and fully absorb water, spreading the cleaned and sterilized pineapple seeds on the water-absorbing sponge uniformly, covering the culture dish, marking, placing the culture dish in a climatic chamber for germination, adding the sterile water timely according to the dry and wet condition of the sponge for 7-10 days, and observing the germination condition of the seeds every day after the germination is carried out for 5 days; timely picking out the broken germinated seeds, placing the broken germinated seeds into a new sponge culture dish B, and culturing the broken germinated seeds and the ungerminated seeds separately for seedling culture so as to prevent the root systems of the germinated seedlings from winding, covering and preventing the ungerminated seeds from developing.
5. The breeding method for comprehensively preventing and controlling seedling diseases of pineapple hybrid seedlings according to claim 1, which is characterized by comprising the following steps: in the step (5), 1 ml-2 ml of improved Hoagland nutrient solution is added for 1 time while the sponge culture dish B is supplemented with sterile water, wherein the improved Hoagland nutrient solution comprises the following components: ca (NO) 3 ) 2 .4H 2 O 900-950mg/L、KNO 3 500-520mg/L、NH 4 NO 3 80-85mg/L、KH 2 PO 4 130-140mg/L、MgSO 4 240-245mg/L、FeNaEDTA 36-37mg/L、KI 0.8-1.0mg/L、H 3 BO 3 6.0-6.5mg/L、MnSO 4 .H 2 O16.5-17.0mg/L、ZnSO 4 .7H 2 O 8.5-9.0mg/L、Na 2 MoO 4 .2H 2 O 0.25mg/L、CuSO 4 .5H 2 O0.025-0.030mg/L、CoCl 2 .6 H 2 O0.025-0.030mg/L, ph=5.8; when in use, the improved Hoagland nutrient solution is diluted by 100 times with distilled water.
6. The breeding method for comprehensively preventing and controlling seedling diseases of pineapple hybrid seedlings according to claim 1, which is characterized by comprising the following steps: and (3) the tweezers used in the steps (6) and (7) are prevented from contacting breeding matrixes and other healthy seedlings, so that new infection is caused, and alcohol lamps are used for burning and thoroughly sterilizing after each use of the tweezers.
7. The breeding method for comprehensively preventing and controlling seedling diseases of pineapple hybrid seedlings according to claim 1, which is characterized by comprising the following steps: in the step (7), 2-3ml of sterilizing liquid is added into the original deformed seedling mildew area for sterilization treatment, and 1-2ml of sterilizing liquid is added according to the growth health condition of the seedling for maintenance.
8. The breeding method for comprehensively preventing and controlling seedling diseases of pineapple hybrid seedlings according to claim 1, which is characterized by comprising the following steps: the sterilizing liquid is 500 times of 50% carbendazim wettable powder or 70% thiophanate methyl 500 times of the sterilizing liquid, and the sterilizing liquid is used alternately.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310460956.9A CN116530261A (en) | 2023-04-26 | 2023-04-26 | Breeding method for comprehensively preventing and controlling pineapple hybrid seedling disease |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310460956.9A CN116530261A (en) | 2023-04-26 | 2023-04-26 | Breeding method for comprehensively preventing and controlling pineapple hybrid seedling disease |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN116530261A true CN116530261A (en) | 2023-08-04 |
Family
ID=87446310
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310460956.9A Pending CN116530261A (en) | 2023-04-26 | 2023-04-26 | Breeding method for comprehensively preventing and controlling pineapple hybrid seedling disease |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN116530261A (en) |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103609313A (en) * | 2013-12-04 | 2014-03-05 | 中国热带农业科学院南亚热带作物研究所 | Pineapple leaf bud cuttage method |
| CN103782848A (en) * | 2013-12-19 | 2014-05-14 | 广西大学 | Method of preventing and treating pineapple mealybug wilting |
| CN104012355A (en) * | 2014-05-29 | 2014-09-03 | 广西南亚热带农业科学研究所 | Jackfruit branch topping and seedling growing method |
| CN106665068A (en) * | 2016-12-09 | 2017-05-17 | 河池市技术开发中心 | Method for fixed planting of pineapple plants high in survival rate |
| CN107624525A (en) * | 2017-09-15 | 2018-01-26 | 广西壮族自治区农业科学院园艺研究所 | A kind of pineapple is gone to a winter resort cultural method |
| CN110012834A (en) * | 2019-04-02 | 2019-07-16 | 云南省热带作物科学研究所 | A method of improving golden eyelet stitch culture efficiency |
| CN114391472A (en) * | 2021-12-08 | 2022-04-26 | 中国热带农业科学院热带作物品种资源研究所 | Method for improving hybrid seed setting rate of pineapples |
| CN114568064A (en) * | 2022-03-01 | 2022-06-03 | 广西南亚热带农业科学研究所 | Pineapple seed breathable water-retention breeding method |
| CN115362932A (en) * | 2022-09-14 | 2022-11-22 | 广西南亚热带农业科学研究所 | Pineapple film-covering moisture-proof hybridization method |
-
2023
- 2023-04-26 CN CN202310460956.9A patent/CN116530261A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103609313A (en) * | 2013-12-04 | 2014-03-05 | 中国热带农业科学院南亚热带作物研究所 | Pineapple leaf bud cuttage method |
| CN103782848A (en) * | 2013-12-19 | 2014-05-14 | 广西大学 | Method of preventing and treating pineapple mealybug wilting |
| CN104012355A (en) * | 2014-05-29 | 2014-09-03 | 广西南亚热带农业科学研究所 | Jackfruit branch topping and seedling growing method |
| CN106665068A (en) * | 2016-12-09 | 2017-05-17 | 河池市技术开发中心 | Method for fixed planting of pineapple plants high in survival rate |
| CN107624525A (en) * | 2017-09-15 | 2018-01-26 | 广西壮族自治区农业科学院园艺研究所 | A kind of pineapple is gone to a winter resort cultural method |
| CN110012834A (en) * | 2019-04-02 | 2019-07-16 | 云南省热带作物科学研究所 | A method of improving golden eyelet stitch culture efficiency |
| CN114391472A (en) * | 2021-12-08 | 2022-04-26 | 中国热带农业科学院热带作物品种资源研究所 | Method for improving hybrid seed setting rate of pineapples |
| CN114568064A (en) * | 2022-03-01 | 2022-06-03 | 广西南亚热带农业科学研究所 | Pineapple seed breathable water-retention breeding method |
| CN115362932A (en) * | 2022-09-14 | 2022-11-22 | 广西南亚热带农业科学研究所 | Pineapple film-covering moisture-proof hybridization method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN111758559B (en) | Sterile sowing and seedling raising method for distant hybrid seeds of phalaenopsis amabilis and rhynchophylla | |
| CN107318553A (en) | A kind of method for culturing seedlings of high-quality yellow peach | |
| CN112931202B (en) | Non-symbiotic germination method for paphiopedilum delavayi seeds | |
| CN105993865A (en) | Cultivation method for quercus variabilis aseptic seedling | |
| CN107646684A (en) | A kind of breeding method of purpleback murdannia herb and its application | |
| CN114568064B (en) | Pineapple seed breathable water-retention breeding method | |
| CN107711508A (en) | A kind of tissue culture and rapid propagation method of dendrobium | |
| CN106577284B (en) | A kind of method of yellow lady's-slipper tissue-culturing rapid propagation and florescence control | |
| CN101268759A (en) | Cultivation method for Dendrobium initial species embryo | |
| CN111919749B (en) | Culture medium for rapid propagation of dendrobium nobile seedlings and rapid propagation method thereof | |
| CN108935105A (en) | A kind of limacia sagittata tissue culture and rapid propagation method | |
| CN106134987A (en) | Exocarpium Citri Rubrum tissue-culturing quick-propagation and method for culturing seedlings | |
| CN105493683B (en) | A kind of processing method after roxburgh anoectochilus terminal bud seed collection | |
| CN105284622B (en) | A kind of method that quick acquisition Rhizoma Iridis Tectori hybridizes superior clone | |
| CN105409748B (en) | A kind of fast breeding method of extra large scirpus scirpus | |
| CN104938088B (en) | Method for promoting germination of caulis sinomenii seeds | |
| CN110622716A (en) | Tomato grafting and seedling raising method | |
| CN113133408B (en) | Method for rapidly breeding ginkgo biloba | |
| CN113826549B (en) | Ornamental dendrobium crossbreeding method | |
| CN116530261A (en) | Breeding method for comprehensively preventing and controlling pineapple hybrid seedling disease | |
| CN109169275A (en) | A kind of pale reddish brown trident bletilla striata tissue-culturing rapid propagation culture medium and method | |
| CN108476982A (en) | A kind of method of sterile rootage breeding in sealwort test tube | |
| CN108651444B (en) | Preservation and breeding method for phalaenopsis pollen | |
| CN116326486B (en) | Method for promoting germination of arnebia euchroma seeds | |
| CN104719151A (en) | Rapid propagation method of dregea sinensis hemsl |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |