CN116499832A - Method and device for detecting carotene content of agricultural products - Google Patents

Method and device for detecting carotene content of agricultural products Download PDF

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Publication number
CN116499832A
CN116499832A CN202310554905.2A CN202310554905A CN116499832A CN 116499832 A CN116499832 A CN 116499832A CN 202310554905 A CN202310554905 A CN 202310554905A CN 116499832 A CN116499832 A CN 116499832A
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carotene
light
wavelength
content
filtrate
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CN116499832B (en
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苏肯明
罗诗泳
李韵仪
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Waltek Services Fo Shan Co ltd
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Waltek Services Fo Shan Co ltd
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
    • G01N21/3103—Atomic absorption analysis

Abstract

The invention provides a method and a device for detecting the carotene content of agricultural products, wherein the method comprises the following steps: cleaning agricultural product sample, wiping and cutting, adding distilled water into tissue masher, mashing into paste, oven drying, and sieving with 80 mesh sieve to obtain pretreated dry powder; placing in a water bath at 45deg.C in the absence of light, extracting with ultrasonic wave at 45deg.C for 3 times each for 15min, filtering, and collecting filtrate; using absolute ethyl alcohol as reference liquid, measuring the extinction degree of the filtrate at the wavelength of 451nm by using a spectrophotometer, and calculating the content of total carotene in the agricultural products; an apparatus, comprising: tissue masher, dryer, ultrasonic equipment and spectrophotometer. The method can clearly and definitely display the result by rapidly measuring the carotene content in the filtrate through the spectrophotometer, thereby omitting the problem of inaccurate carotene content measurement caused by manual operation and effectively improving the monitoring of carotene in agricultural products.

Description

Method and device for detecting carotene content of agricultural products
Technical Field
The invention relates to the technical field of food detection, in particular to a method and a device for detecting the carotene content of agricultural products.
Background
Carotene is a necessary nutrient for human body, and proper amount of carotene can be taken through diet in daily life; the carotene is named vitamin A, which is one of the indispensable nutrient elements in the growth and development process of human body, the food material rich in carotene mainly takes orange and green vegetables, such as carrots, leeks, rapes or spinach, and the like, and the food rich in carotene belongs to carrots. The carotene has effects of promoting normal growth and reproduction of organism, maintaining epithelial tissue, preventing respiratory tract infection and keeping vision normal, and treating nyctalopia and xerophthalmia. The vitamins required by the human body can be timely supplemented by frequently eating some fruits and vegetables.
In the first prior art, application No. cn202211564410.X discloses a method for determining the content of 13 carotenoids in sweet potatoes, comprising the steps of: pretreating a sample to be detected to obtain a sample solution to be on-machine; respectively preparing standard solutions of violaxanthin, neoxanthin, epoxy zeaxanthin, lutein, zeaxanthin, alpha-cryptoxanthin, epsilon-carotene, beta-cryptoxanthin, alpha-carotene, beta-carotene, delta-carotene, gamma-carotene and lycopene, and drawing a standard curve; and carrying out ultra-high performance liquid chromatography detection on the sample solution to be on-line, and substituting the peak areas of the obtained components into a standard curve to obtain the concentrations of the 13 components in the sample to be detected. Although the 13 carotenoid linear relation can be determined, the precision, the repeatability, the stability and the recovery rate result meet the requirements, and a foundation can be laid for quality analysis of mass sweet potato germplasm resources and breeding materials; however, the operation steps are complicated, the mixing speed of the sample solution is low, and the detection efficiency is reduced to a certain extent.
In the second prior art, application number CN202010640651.2 discloses a method for measuring the content of beta-carotene, and a one-step method is adopted in pretreatment of the invention to pretreat, saponify and extract a sample to be detected; the mobile phase adopted by the high performance liquid chromatography is 100% methanol. The method has the advantages that the beta-carotene and the content thereof are detected by adopting a high performance liquid chromatography, the pretreatment processes of pretreatment, saponification and extraction can be completed once in the pretreatment process of a sample, the step operation is not needed, the operation is simple, the time is short, the technical problems that the organic phase is lost when the organic phase is dehydrated, the saponification is incomplete, the emulsification phenomenon occurs when the extraction is carried out, the delamination is difficult, the treatment time is too long are solved, and the use of an easy-to-poison reagent as a mobile phase in the detection is avoided; however, only methanol is used as a mobile phase, resulting in poor detection accuracy of carotene.
In the third prior art, CN202010123898.7 discloses a method for measuring the carotenoid content in red-heart duck eggs, which belongs to the field of chromatographic detection; the method comprises the steps of extracting a sample through acetonitrile, concentrating, degreasing by normal hexane, separating by adopting a Agilent Poroshell EC-C18 (4 mu m,4.6mm multiplied by 100 mm) chromatographic column, performing gradient elution by taking 20mmol/L ammonium acetate and acetonitrile as mobile phases, controlling the flow rate of the mobile phases to be 1.0mL/min, detecting the detection wavelength of a diode array detector to be 471nm, and completing the determination of carotenoid content in red-heart duck eggs in 25 min. Although the operation is simple, the separation degree is good, the sensitivity and the accuracy are high, and a method reference is provided for measuring the carotenoid content in the red-heart duck eggs; however, the range of the carotene to be measured is limited, and only carotenoids are aimed at, so that the total content of carotenes in agricultural products cannot be effectively reacted.
At present, the first, the second and the third existing technologies lack effective pretreatment of agricultural product test samples, resulting in poor purity of the test samples; the invention provides a detection method and a detection device for the carotene content of agricultural products, which are characterized in that agricultural product samples are preprocessed to obtain dry powder, ultrasonic extraction is used, filtering is carried out, filtrate is collected, the extinction degree of the filtrate is measured at the wavelength of 451nm by a spectrophotometer, and the total carotene content in the agricultural products is calculated.
Disclosure of Invention
In order to solve the technical problems, the invention provides a method for detecting the carotene content of agricultural products, which comprises the following steps:
cleaning agricultural product sample, wiping and cutting, adding distilled water into tissue masher, mashing into paste, oven drying, and sieving with 80 mesh sieve to obtain pretreated dry powder;
placing in water bath in dark place, extracting with ultrasonic wave at 45deg.C for 3 times each for 15min, filtering, and collecting filtrate;
absolute ethyl alcohol is used as reference liquid, spectrophotometry is used for measuring the extinction degree of filtrate at the wavelength of 451nm, and the content of total carotene in agricultural products is calculated.
Optionally, the control process of the motor rotation speed in the tissue masher comprises the following steps:
the controller measures the motor driving current at a certain moment and obtains the motor rotation speed at a certain moment according to the rotation speed corresponding to the preset motor driving current;
inputting the current rotation speed, the preset motor driving current and the motor rotation speed corresponding to the preset motor driving current into a nonlinear neural network model, and calculating and comparing the current rotation speed and the motor rotation speed corresponding to the preset motor driving current;
and outputting a corrected rotation speed instruction corresponding to the current motor driving current according to the comparison result of the nonlinear neural network model, inputting a proportional-integral-derivative PID controller, and adjusting the motor rotation speed to correspond to the preset motor driving current.
Optionally, petroleum ether and normal hexane are adopted as extraction solvents in ultrasonic extraction, and the mass ratio of the extraction solvents to the feed liquid of the dry powder is 3:7, petroleum ether according to the mass ratio: n-hexane=1: 2.
optionally, the process of measuring the extinction of the filtrate by a spectrophotometer comprises the steps of:
the laser of the spectrophotometer emits light with the wavelength of 451nm and corrects the light with the wavelength of 451nm;
placing the filtrate in a light path of light with the wavelength of 451nm, enabling the transmitted light beam to irradiate on a test light detector, and simultaneously collecting a reference liquid and a light intensity signal value of the light with the wavelength of 451nm by using a data collector;
and calculating to obtain the light absorption value of the filtrate, and converting the light absorption value into the concentration value of carotene in the agricultural products, namely the content value of carotene in the agricultural products.
Optionally, the process of correcting the light with the wavelength of 451nm comprises the following steps:
obtaining a characteristic peak wavelength of an emission spectrum and a transmission spectrum of a light source by using a spectrophotometer, wherein the characteristic peak wavelength is 451nm;
the method comprises the steps of obtaining diode marks of characteristic peaks by using a diode array detector, scanning energy values of each photodiode, filling a blank solution in a detection pool, scanning the energy values, filling filtrate, scanning the energy values again to obtain absorbance, and plotting by taking the diode marks as abscissa and the absorbance as ordinate to obtain an absorption spectrum of an object to be detected;
the diode mark is converted into an accurate wavelength value, and compared with 451nm, and the wavelength is consistent, so that the calibration of the wavelength of the detector is realized.
Optionally, the process of collecting the light intensity signal value by the data collector comprises the following steps:
collecting a reference liquid emitted by a light detector and a light intensity signal value of light with the wavelength of 451nm;
filtering interference components in the light intensity signal value; a part of the light intensity signal value enters a reference signal generator to generate a reference signal, and the other part enters a demodulator;
the demodulator performs multiplication operation on the light intensity signal value and the reference signal, and separates the frequency of the modulated signal in the modulated light intensity signal value and the raster scanning frequency;
the power of the photodetector is increased or decreased according to the modulation signal frequency adjustment.
Optionally, the calculation process of the concentration value of carotene in the agricultural product comprises the following steps:
measuring the light absorption value of a standard carotene solution under the light with the wavelength of 451nm, and establishing a standard curve according to the corresponding relation between the light absorption value and the carotene concentration;
the calculated light absorption value of the filtrate is transmitted to a processor to be compared with a standard curve, so that the concentration of carotene in the current agricultural product is obtained;
and (5) converting the content of carotene in the agricultural products according to the dilution multiple of the filtrate.
The invention provides a device for detecting the carotene content of agricultural products, which comprises:
a tissue masher for mashing the shredded agricultural product sample;
the dryer is responsible for drying the smashed agricultural product samples to obtain dry powder of the agricultural product samples;
the ultrasonic equipment is responsible for carrying out ultrasonic extraction on the dry powder at 45 ℃ for 3 times under the conditions of 15min each time, filtering and collecting filtrate;
and a spectrophotometer for measuring the extinction of the filtrate at 451nm wavelength by taking absolute ethyl alcohol as a reference liquid, and calculating the content of total carotene in the agricultural product.
Optionally, the spectrophotometer comprises:
the data acquisition device is responsible for simultaneously acquiring a reference liquid and a light intensity signal value of light with the wavelength of 451nm;
the light correction module is responsible for obtaining characteristic peak wavelength of the light source emission spectrum and the transmission spectrum by utilizing a spectrophotometer, wherein the characteristic peak wavelength is 451nm; the method comprises the steps of obtaining diode marks of characteristic peaks by using a diode array detector, scanning energy values of each photodiode, filling a blank solution in a detection pool, scanning the energy values, filling filtrate, scanning the energy values again to obtain absorbance, and plotting by taking the diode marks as abscissa and the absorbance as ordinate to obtain an absorption spectrum of an object to be detected; converting the diode mark into an accurate wavelength value, comparing the accurate wavelength value with 451nm, and calibrating the wavelength of the detector when the wavelength is consistent;
the content calculation module is responsible for placing the filtrate in a light path of light with the wavelength of 451nm, calculating to obtain the light absorption value of the filtrate, and converting the light absorption value into the concentration value of carotene in the agricultural products, namely the content value of carotene in the agricultural products.
Optionally, the content calculating module includes:
the standard curve establishing module is responsible for measuring the light absorption value of the standard carotene solution under the light with the wavelength of 451nm and establishing a standard curve according to the corresponding relation between the light absorption value and the carotene concentration;
the component concentration calculation module is in charge of transmitting the calculated light absorption value of the filtrate to a processor to be compared with a standard curve, so as to obtain the concentration of carotene in the current agricultural product;
the component content calculating module is responsible for converting the content of carotene in the agricultural products according to the dilution multiple of the filtrate.
Firstly, cleaning, wiping and cutting an agricultural product sample, adding the agricultural product sample into a distilled water tissue masher to be mashed into paste, and drying the paste and sieving the paste through a 80-mesh sieve to obtain pretreated dry powder; then placing in a water bath at 45deg.C in the dark, extracting with ultrasonic wave at 45deg.C for 3 times each for 15min, filtering, and collecting filtrate; finally, absolute ethyl alcohol is used as reference liquid, spectrophotometry is used for measuring the extinction degree of filtrate at the wavelength of 451nm, and the content of total carotene in the agricultural products is calculated; according to the scheme, the carrot sample is pretreated to obtain the high-quality dry powder with higher purity, so that the content detection precision of carotene is improved, and the influence of other substances on carotene measurement is reduced; the ultrasonic extraction improves the time for the extraction solvent to quickly enter the dry powder, shortens the fusion time of the extraction solvent and the dry powder, shortens the time for obtaining the mixed solution, and can effectively improve the efficiency of the spectrophotometer; the method has the advantages that the content of carotene in the filtrate is rapidly determined through the spectrophotometer, the result can be clearly and clearly displayed, the measurement is performed at the wavelength 451nm, the problem that the content of carotene is inaccurately measured due to manual operation is solved, meanwhile, the intelligent detection capability of detection is improved, the monitoring of carotene in agricultural products is effectively improved, the change and improvement of planting measures during planting are facilitated, and the economic benefit of planting the agricultural products is improved.
Additional features and advantages of the invention will be set forth in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention. The objectives and other advantages of the invention will be realized and attained by the structure particularly pointed out in the written description and claims thereof as well as the appended drawings.
The technical scheme of the invention is further described in detail through the drawings and the embodiments.
Drawings
The accompanying drawings are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate the invention and together with the embodiments of the invention, serve to explain the invention. In the drawings:
FIG. 1 is a flow chart showing a method for detecting the carotene content of an agricultural product according to example 1 of the present invention;
FIG. 2 is a diagram showing the control of the rotational speed of the motor in the tissue morcellator according to example 2 of the present invention;
FIG. 3 is a diagram showing the process of measuring the extinction of filtrate by a spectrophotometer in example 4 of the present invention;
FIG. 4 is a diagram showing the process of correcting a light having a wavelength of 451nm in example 5 of the present invention;
FIG. 5 is a diagram showing a process of collecting light intensity signal values by the data collector in embodiment 6 of the present invention;
FIG. 6 is a graph showing the calculation of the concentration value of carotene in agricultural products in example 7 of the present invention;
FIG. 7 is a block diagram showing a device for detecting the carotene content of an agricultural product in example 8 of the present invention;
FIG. 8 is a block diagram of a spectrophotometer in example 9 of the present invention;
fig. 9 is a block diagram of a content calculating module in embodiment 10 of the present invention.
Detailed Description
The preferred embodiments of the present invention will be described below with reference to the accompanying drawings, it being understood that the preferred embodiments described herein are for illustration and explanation of the present invention only, and are not intended to limit the present invention.
The terminology used in the embodiments of the application is for the purpose of describing particular embodiments only and is not intended to be limiting of the embodiments of the application. As used in this application and the appended claims, the singular forms "a," "an," and "the" are intended to include the plural forms as well, unless the context clearly indicates otherwise. It should also be understood that the term "and/or" as used herein refers to and encompasses any or all possible combinations of one or more of the associated listed items.
When the following description refers to the accompanying drawings, the same numbers in different drawings refer to the same or similar elements, unless otherwise indicated. The implementations described in the following exemplary examples are not representative of all implementations consistent with the present application. Rather, they are merely examples of apparatus and methods consistent with some aspects of the present application as detailed in the accompanying claims. The specific meaning of the terms in this application will be understood by those of ordinary skill in the art as the case may be.
Example 1: as shown in fig. 1, the embodiment of the invention provides a method for detecting the carotene content of agricultural products, which comprises the following steps:
s100: cleaning agricultural product sample, wiping and cutting, adding distilled water into tissue masher, mashing into paste, oven drying, and sieving with 80 mesh sieve to obtain pretreated dry powder;
s200: placing in a water bath at 45deg.C in the absence of light, extracting with ultrasonic wave at 45deg.C for 3 times each for 15min, filtering, and collecting filtrate;
s300: using absolute ethyl alcohol as reference liquid, measuring the extinction degree of the filtrate at the wavelength of 451nm by using a spectrophotometer, and calculating the content of total carotene in the agricultural products;
the working principle and beneficial effects of the technical scheme are as follows: firstly, washing, wiping and cutting an agricultural product sample, adding the agricultural product sample into a distilled water tissue masher to be mashed into paste, and drying the paste and sieving the paste through a 80-mesh sieve to obtain pretreated dry powder; then placing in a water bath at 45deg.C in the dark, extracting with ultrasonic wave at 45deg.C for 3 times each for 15min, filtering, and collecting filtrate; finally, absolute ethyl alcohol is used as reference liquid, spectrophotometry is used for measuring the extinction degree of filtrate at the wavelength of 451nm, and the content of total carotene in the agricultural products is calculated; according to the scheme, the carrot sample is pretreated to obtain the high-quality dry powder with higher purity, so that the content detection precision of carotene is improved, and the influence of other substances on carotene measurement is reduced; the ultrasonic extraction improves the time for the extraction solvent to quickly enter the dry powder, shortens the fusion time of the extraction solvent and the dry powder, shortens the time for obtaining the mixed solution, and can effectively improve the efficiency of the spectrophotometer; the method has the advantages that the content of carotene in the filtrate is rapidly determined through the spectrophotometer, the result can be clearly and clearly displayed, the measurement is performed at the wavelength 451nm, the problem that the content of carotene is inaccurately measured due to manual operation is solved, meanwhile, the intelligent detection capability of detection is improved, the monitoring of carotene in agricultural products is effectively improved, the change and improvement of planting measures during planting are facilitated, and the economic benefit of planting the agricultural products is improved.
Example 2: as shown in fig. 2, on the basis of embodiment 1, the control process of the motor rotation speed in the tissue masher provided by the embodiment of the invention comprises the following steps:
s101: the controller measures the motor driving current at a certain moment and obtains the motor rotation speed at a certain moment according to the rotation speed corresponding to the preset motor driving current;
s102: inputting the current rotation speed, the preset motor driving current and the motor rotation speed corresponding to the preset motor driving current into a nonlinear neural network model, and calculating and comparing the current rotation speed and the motor rotation speed corresponding to the preset motor driving current;
s103: outputting a corrected rotation speed instruction corresponding to the current motor driving current according to the comparison result of the nonlinear neural network model, inputting a proportional-integral-derivative PID controller, and adjusting the motor rotation speed to correspond to the preset motor driving current;
the working principle and beneficial effects of the technical scheme are as follows: the method comprises the steps that firstly, a controller measures motor driving current at a certain moment, and the motor rotating speed at a certain moment is obtained according to the rotating speed corresponding to the preset motor driving current; secondly, inputting the current rotation speed, the preset motor driving current and the motor rotation speed corresponding to the preset motor driving current into a nonlinear neural network model, and calculating and comparing the current rotation speed and the motor rotation speed corresponding to the preset motor driving current; finally, outputting a corrected rotation speed instruction corresponding to the current motor driving current according to the comparison result nonlinear neural network model, inputting a proportional-integral-derivative PID controller, and adjusting the motor rotation speed to correspond to the preset motor driving current; according to the scheme, through corresponding adjustment of the motor rotation speed and the motor driving current, on one hand, the motor of the tissue masher is ensured to be in a high-efficiency working efficiency, on the other hand, the mashing effect of agricultural product samples is ensured, and the purity of the agricultural product pretreatment dry powder can be ensured; meanwhile, the motor rotation speed of the tissue masher is automatically controlled, the accuracy of carotene content detection is improved, and the agricultural product sample processing time in the carotene content detection process is shortened.
Example 3: based on the embodiment 1, the extraction solvent in the ultrasonic extraction provided by the embodiment of the invention adopts petroleum ether and normal hexane, and the mass ratio of the extraction solvent to the feed liquid of the dry powder is 3:7, petroleum ether according to the mass ratio: n-hexane=1: 2;
the working principle and beneficial effects of the technical scheme are as follows: the extraction solvent in the ultrasonic extraction of this embodiment adopts petroleum ether and n-hexane, and the mass ratio of the extraction solvent to the feed liquid of the dry powder is 3:7, petroleum ether according to the mass ratio: n-hexane=1: 2; according to the scheme, the petroleum ether and the n-hexane are adopted as the extraction solvents, and the elution intensities of the petroleum ether and the n-hexane are inconsistent, so that the extraction quality of carotene in ultrasonic waves can be improved by using the extraction solvents with different elution degrees, and the accuracy of the detection result of the carotene content in agricultural products can be effectively ensured; petroleum ether and n-hexane 1 were used: 2, the dissolution efficiency of the carotene is improved, and the dissolution rate of the carotene in the agricultural products is ensured.
Example 4: as shown in fig. 3, on the basis of example 1, the process of measuring the extinction of the filtrate by the spectrophotometer provided by the embodiment of the invention comprises the following steps:
s301: the laser of the spectrophotometer emits light with the wavelength of 451nm and corrects the light with the wavelength of 451nm;
s302: placing the filtrate in a light path of light with the wavelength of 451nm, enabling the transmitted light beam to irradiate on a test light detector, and simultaneously collecting a reference liquid and a light intensity signal value of the light with the wavelength of 451nm by using a data collector;
s303: calculating to obtain the light absorption value of the filtrate, and converting the light absorption value into the concentration value of carotene in the agricultural products, namely the content value of carotene in the agricultural products;
the working principle and beneficial effects of the technical scheme are as follows: in the embodiment, a laser of a spectrophotometer emits light with the wavelength of 451nm, and corrects the light with the wavelength of 451nm; secondly, placing the filtrate in a light path of light with the wavelength of 451nm, enabling the transmitted light beam to irradiate on a test light detector, and simultaneously collecting a reference liquid and a light intensity signal value of the light with the wavelength of 451nm by using a data collector; finally, calculating to obtain the light absorption value of the filtrate, and converting the light absorption value into the concentration value of carotene in the agricultural products, namely the content value of carotene in the agricultural products; according to the scheme, the light absorption value of the filtrate is measured through the spectrophotometer, the content value of carotene in the agricultural products is obtained through calculation, the measuring process is simple and easy to operate, and the carotene is measured through the light with the wavelength of 451nm, so that the precise measurement of the carotene is realized, the interference of other substances is eliminated, and the detection precision is improved.
Example 5: as shown in fig. 4, on the basis of embodiment 4, the process for correcting the light with the wavelength of 451nm provided in the embodiment of the present invention includes the following steps:
s3011: obtaining a characteristic peak wavelength of an emission spectrum and a transmission spectrum of a light source by using a spectrophotometer, wherein the characteristic peak wavelength is 451nm;
s3012: the method comprises the steps of obtaining diode marks of characteristic peaks by using a diode array detector, scanning energy values of each photodiode, filling a blank solution in a detection pool, scanning the energy values, filling filtrate, scanning the energy values again to obtain absorbance, and plotting by taking the diode marks as abscissa and the absorbance as ordinate to obtain an absorption spectrum of an object to be detected;
s3013: converting the diode mark into an accurate wavelength value, comparing the accurate wavelength value with 451nm, and calibrating the wavelength of the detector when the wavelength is consistent;
the working principle and beneficial effects of the technical scheme are as follows: in the embodiment, a spectrophotometer is used for obtaining the characteristic peak wavelength of the light source emission spectrum and the transmission spectrum, wherein the characteristic peak wavelength is 451nm; secondly, a diode array detector is utilized to obtain a diode mark of a characteristic peak, and a functional relation between the characteristic wavelength value and the diode mark is fitted according to the characteristic wavelength value and the diode mark; scanning the energy value of each photodiode, filling a blank solution in a detection tank, scanning the energy value, filling filtrate, and scanning the energy value again to obtain absorbance, and plotting with the diode mark as an abscissa and the absorbance as an ordinate to obtain an absorption spectrum of an object to be detected; finally, converting the diode mark into an accurate wavelength value, comparing the accurate wavelength value with 451nm, and calibrating the wavelength of the detector when the wavelength is consistent; according to the scheme, through wavelength calibration, the accuracy of carotene content detection is ensured, the detection error is reduced to the minimum, and the accuracy of carotene content detection in agricultural products is ensured; in addition, the comparison method of absorbance is simple, the operation is convenient and direct, and the accuracy of the light rays with the wavelength of 451nm is greatly improved.
Example 6: as shown in fig. 5, on the basis of embodiment 4, the process of collecting the light intensity signal value by the data collector provided by the embodiment of the invention includes the following steps:
s3021: collecting a reference liquid emitted by a light detector and a light intensity signal value of light with the wavelength of 451nm;
s3022: filtering interference components in the light intensity signal value; a part of the light intensity signal value enters a reference signal generator to generate a reference signal, and the other part enters a demodulator;
s3023: the demodulator performs multiplication operation on the light intensity signal value and the reference signal, and separates the frequency of the modulated signal in the modulated light intensity signal value and the raster scanning frequency;
s3024: increasing or reducing the power of the optical detector according to the frequency adjustment of the modulation signal;
the working principle and beneficial effects of the technical scheme are as follows: in the embodiment, firstly, the reference liquid emitted by the light detector and the light intensity signal value of the light with the wavelength of 451nm are collected; secondly, filtering interference components in the light intensity signal value; a part of the light intensity signal value enters a reference signal generator to generate a reference signal, and the other part enters a demodulator; the demodulator performs multiplication operation on the light intensity signal value and the reference signal, and separates the modulation signal frequency and the raster scanning frequency in the modulation light intensity signal value; finally, adjusting and increasing or reducing the power of the optical detector according to the frequency of the modulation signal; according to the scheme, the interference component in the light intensity signal value is filtered through processing the light intensity signal value, the precision of the light intensity signal value is improved, in addition, the light intensity signal value is adjusted according to the frequency of the modulation signal, a reference basis is provided for power adjustment of the optical detector, the precision of the output light intensity signal value is ensured, and light rays with the wavelength meeting the requirement are ensured to be emitted.
Example 7: as shown in fig. 6, on the basis of example 4, the calculation process of the concentration value of carotene in agricultural products provided by the embodiment of the invention includes the following steps:
s3031: measuring the light absorption value of a standard carotene solution under the light with the wavelength of 451nm, and establishing a standard curve according to the corresponding relation between the light absorption value and the carotene concentration;
s3032: the calculated light absorption value of the filtrate is transmitted to a processor to be compared with a standard curve, so that the concentration of carotene in the current agricultural product is obtained;
s3033: the carotene content in the agricultural products is converted according to the dilution multiple of the filtrate;
the working principle and beneficial effects of the technical scheme are as follows: in the embodiment, firstly, under the light with the wavelength of 451nm, the light absorption value of a standard carotene solution is measured, and a standard curve is established according to the corresponding relation between the light absorption value and the carotene concentration; secondly, the calculated light absorption value of the filtrate is transmitted to a processor to be compared with a standard curve, so that the concentration of carotene in the current agricultural product is obtained; finally, according to the dilution multiple of the filtrate, the content of carotene in the agricultural product is converted, the light absorption value of the filtrate is calculated, and the light absorption value is converted into the concentration value of the carotene in the agricultural product, namely the content value of the carotene in the agricultural product; the scheme establishes the standard curve, improves the time for quickly obtaining the concentration value according to the light absorption value, automatically searches by the processor, improves the searching efficiency and speed of the concentration value, and reduces the error of concentration value calculation; the carotene content in the agricultural products is calculated through the concentration value, so that a reference is provided for the planting measure and scale adjustment of the agricultural products, and the carotene content in the agricultural products is improved; the method has the advantages of accurate detection result, strong pertinence, simple and convenient operation, good stability, easy control and good repeatability.
Example 8: as shown in fig. 7, on the basis of examples 1 to 7, the apparatus for detecting the carotene content of agricultural products according to the present invention comprises:
a tissue masher for mashing the shredded agricultural product sample;
the dryer is responsible for drying the smashed agricultural product samples to obtain dry powder of the agricultural product samples;
the ultrasonic equipment is responsible for carrying out ultrasonic extraction on the dry powder at 45 ℃ for 3 times under the conditions of 15min each time, filtering and collecting filtrate;
the spectrophotometer is responsible for measuring the extinction of the filtrate at the wavelength of 451nm by taking absolute ethyl alcohol as a reference liquid, and calculating the content of total carotene in the agricultural products;
the working principle and beneficial effects of the technical scheme are as follows: the tissue morcellator of this embodiment morcellates a shredded agricultural product sample; the dryer dries the smashed agricultural product sample to obtain dry powder of the agricultural product sample; carrying out ultrasonic extraction on the dry powder at 45 ℃ for 3 times under the conditions of 15min each time, and filtering and collecting filtrate; the spectrophotometer takes absolute ethyl alcohol as reference liquid, the spectrophotometer is used for measuring the extinction of filtrate at the wavelength of 451nm, and the content of total carotene in the agricultural products is calculated; according to the scheme, the carrot sample is pretreated to obtain the high-quality dry powder with higher purity, so that the content detection precision of carotene is improved, and the influence of other substances on carotene measurement is reduced; the ultrasonic extraction improves the time for the extraction solvent to quickly enter the dry powder, shortens the fusion time of the extraction solvent and the dry powder, shortens the time for obtaining the mixed solution, and can effectively improve the efficiency of the spectrophotometer; the method has the advantages that the content of carotene in the filtrate is rapidly determined through the spectrophotometer, the result can be clearly and clearly displayed, the measurement is performed at the wavelength 451nm, the problem that the content of carotene is inaccurately measured due to manual operation is solved, meanwhile, the intelligent detection capability of detection is improved, the monitoring of carotene in agricultural products is effectively improved, the change and improvement of planting measures during planting are facilitated, and the economic benefit of planting the agricultural products is improved.
Example 9: as shown in fig. 8, on the basis of example 8, the spectrophotometer provided in the embodiment of the present invention includes:
the data acquisition device is responsible for simultaneously acquiring a reference liquid and a light intensity signal value of light with the wavelength of 451nm;
the light correction module is responsible for obtaining characteristic peak wavelength of the light source emission spectrum and the transmission spectrum by utilizing a spectrophotometer, wherein the characteristic peak wavelength is 451nm; the method comprises the steps of obtaining diode marks of characteristic peaks by using a diode array detector, scanning energy values of each photodiode, filling a blank solution in a detection pool, scanning the energy values, filling filtrate, scanning the energy values again to obtain absorbance, and plotting by taking the diode marks as abscissa and the absorbance as ordinate to obtain an absorption spectrum of an object to be detected; converting the diode mark into an accurate wavelength value, comparing the accurate wavelength value with 451nm, and calibrating the wavelength of the detector when the wavelength is consistent;
the content calculation module is responsible for placing the filtrate in a light path of light with the wavelength of 451nm, calculating to obtain a light absorption value of the filtrate, and converting the light absorption value into a concentration value of carotene in the agricultural product, namely a content value of the carotene in the agricultural product;
the working principle and beneficial effects of the technical scheme are as follows: the data acquisition device of the embodiment acquires the reference liquid and the light intensity signal value of the light with the wavelength of 451nm at the same time; the light correction module obtains a characteristic peak wavelength of the light source emission spectrum and the transmission spectrum by utilizing a spectrophotometer, wherein the characteristic peak wavelength is 451nm; the method comprises the steps of obtaining diode marks of characteristic peaks by using a diode array detector, scanning energy values of each photodiode, filling a blank solution in a detection pool, scanning the energy values, filling filtrate, scanning the energy values again to obtain absorbance, and plotting by taking the diode marks as abscissa and the absorbance as ordinate to obtain an absorption spectrum of an object to be detected; converting the diode mark into an accurate wavelength value, comparing the accurate wavelength value with 451nm, and calibrating the wavelength of the detector when the wavelength is consistent; the content calculation module is used for placing the filtrate in a light path of light with the wavelength of 451nm, calculating to obtain a light absorption value of the filtrate, and converting the light absorption value into a concentration value of carotene in the agricultural product, namely a content value of carotene in the agricultural product; according to the scheme, the light absorption value of the filtrate is measured through the spectrophotometer, the content value of carotene in the agricultural products is obtained through calculation, the measuring process is simple and easy to operate, and the carotene is measured through the light with the wavelength of 451nm, so that the precise measurement of the carotene is realized, the interference of other substances is eliminated, and the detection precision is improved; the accuracy of carotene content detection is ensured by wavelength calibration, the detection error is reduced to the minimum, and the accuracy of carotene content detection in agricultural products is ensured; in addition, the comparison method of absorbance is simple, the operation is convenient and direct, and the accuracy of the light rays with the wavelength of 451nm is greatly improved.
Example 10: as shown in fig. 9, on the basis of embodiment 8, the content calculation module provided in the embodiment of the present invention includes:
the standard curve establishing module is responsible for measuring the light absorption value of the standard carotene solution under the light with the wavelength of 451nm and establishing a standard curve according to the corresponding relation between the light absorption value and the carotene concentration;
the component concentration calculation module is in charge of transmitting the calculated light absorption value of the filtrate to a processor to be compared with a standard curve, so as to obtain the concentration of carotene in the current agricultural product;
the component content calculating module is responsible for converting the content of carotene in the agricultural products according to the dilution multiple of the filtrate;
the working principle and beneficial effects of the technical scheme are as follows: the standard curve establishing module of the embodiment determines the light absorption value of the standard carotene solution under the light with the wavelength of 451nm, and establishes a standard curve according to the corresponding relation between the light absorption value and the carotene concentration; the component concentration calculation module transmits the calculated light absorption value of the filtrate to the processor to be compared with a standard curve, so as to obtain the concentration of carotene in the current agricultural product; the component content calculating module converts the content of carotene in the agricultural products according to the dilution multiple of the filtrate; the scheme establishes the standard curve, improves the time for quickly obtaining the concentration value according to the light absorption value, automatically searches by the processor, improves the searching efficiency and speed of the concentration value, and reduces the error of concentration value calculation; the carotene content in the agricultural products is calculated through the concentration value, so that a reference is provided for the planting measure and scale adjustment of the agricultural products, and the carotene content in the agricultural products is improved; the method has the advantages of accurate detection result, strong pertinence, simple and convenient operation, good stability, easy control and good repeatability.
It will be apparent to those skilled in the art that various modifications and variations can be made to the present invention without departing from the spirit or scope of the invention. Thus, it is intended that the present invention also include such modifications and alterations insofar as they come within the scope of the appended claims or the equivalents thereof.

Claims (10)

1. A method for detecting the carotene content of agricultural products, comprising the steps of:
cleaning agricultural product sample, wiping and cutting, adding distilled water into tissue masher, mashing into paste, oven drying, and sieving with 80 mesh sieve to obtain pretreated dry powder;
placing in water bath in dark place, extracting with ultrasonic wave at 45deg.C for 3 times each for 15min, filtering, and collecting filtrate;
absolute ethyl alcohol is used as reference liquid, spectrophotometry is used for measuring the extinction degree of filtrate at the wavelength of 451nm, and the content of total carotene in agricultural products is calculated.
2. A method for detecting the carotene content of agricultural products according to claim 1, wherein the control process of the rotational speed of the motor in the tissue masher comprises the steps of:
the controller measures the motor driving current at a certain moment and obtains the motor rotation speed at a certain moment according to the rotation speed corresponding to the preset motor driving current;
inputting the current rotation speed, the preset motor driving current and the motor rotation speed corresponding to the preset motor driving current into a nonlinear neural network model, and calculating and comparing the current rotation speed and the motor rotation speed corresponding to the preset motor driving current;
and outputting a corrected rotation speed instruction corresponding to the current motor driving current according to the comparison result of the nonlinear neural network model, inputting a proportional-integral-derivative PID controller, and adjusting the motor rotation speed to correspond to the preset motor driving current.
3. The method for detecting the carotene content of agricultural products according to claim 1, wherein the extracting solvent in the ultrasonic extraction adopts petroleum ether and n-hexane, and the mass ratio of the extracting solvent to the dry powder is 3:7, petroleum ether according to the mass ratio: n-hexane=1: 2.
4. a method for detecting the carotene content of agricultural products according to claim 1, wherein the process of measuring the extinction of the filtrate by a spectrophotometer comprises the steps of:
the laser of the spectrophotometer emits light with the wavelength of 451nm and corrects the light with the wavelength of 451nm;
placing the filtrate in a light path of light with the wavelength of 451nm, enabling the transmitted light beam to irradiate on a test light detector, and simultaneously collecting a reference liquid and a light intensity signal value of the light with the wavelength of 451nm by using a data collector;
and calculating to obtain the light absorption value of the filtrate, and converting the light absorption value into the concentration value of carotene in the agricultural products, namely the content value of carotene in the agricultural products.
5. The method for detecting the carotene content of agricultural products according to claim 4, wherein the process of correcting the light having the wavelength of 451nm comprises the steps of:
obtaining a characteristic peak wavelength of an emission spectrum and a transmission spectrum of a light source by using a spectrophotometer, wherein the characteristic peak wavelength is 451nm;
the method comprises the steps of obtaining diode marks of characteristic peaks by using a diode array detector, scanning energy values of each photodiode, filling a blank solution in a detection pool, scanning the energy values, filling filtrate, scanning the energy values again to obtain absorbance, and plotting by taking the diode marks as abscissa and the absorbance as ordinate to obtain an absorption spectrum of an object to be detected;
the diode mark is converted into an accurate wavelength value, and compared with 451nm, and the wavelength is consistent, so that the calibration of the wavelength of the detector is realized.
6. A method for detecting the carotene content of agricultural products according to claim 4, wherein the process of collecting the light intensity signal value by the data collector comprises the steps of:
collecting a reference liquid emitted by a light detector and a light intensity signal value of light with the wavelength of 451nm;
filtering interference components in the light intensity signal value; a part of the light intensity signal value enters a reference signal generator to generate a reference signal, and the other part enters a demodulator;
the demodulator performs multiplication operation on the light intensity signal value and the reference signal, and separates the frequency of the modulated signal in the modulated light intensity signal value and the raster scanning frequency;
the power of the photodetector is increased or decreased according to the modulation signal frequency adjustment.
7. The method for detecting the carotene content of agricultural products according to claim 4, wherein the calculation process of the concentration value of carotene in the agricultural products comprises the steps of:
measuring the light absorption value of a standard carotene solution under the light with the wavelength of 451nm, and establishing a standard curve according to the corresponding relation between the light absorption value and the carotene concentration;
the calculated light absorption value of the filtrate is transmitted to a processor to be compared with a standard curve, so that the concentration of carotene in the current agricultural product is obtained;
and (5) converting the content of carotene in the agricultural products according to the dilution multiple of the filtrate.
8. A device for detecting the carotene content of agricultural products, comprising:
a tissue masher for mashing the shredded agricultural product sample;
the dryer is responsible for drying the smashed agricultural product samples to obtain dry powder of the agricultural product samples;
the ultrasonic equipment is responsible for carrying out ultrasonic extraction on the dry powder at 45 ℃ for 3 times under the conditions of 15min each time, filtering and collecting filtrate;
and a spectrophotometer for measuring the extinction of the filtrate at 451nm wavelength by taking absolute ethyl alcohol as a reference liquid, and calculating the content of total carotene in the agricultural product.
9. The agricultural product carotene content detection device of claim 8, wherein the spectrophotometer comprises:
the data acquisition device is responsible for simultaneously acquiring a reference liquid and a light intensity signal value of light with the wavelength of 451nm;
the light correction module is responsible for obtaining characteristic peak wavelength of the light source emission spectrum and the transmission spectrum by utilizing a spectrophotometer, wherein the characteristic peak wavelength is 451nm; the method comprises the steps of obtaining diode marks of characteristic peaks by using a diode array detector, scanning energy values of each photodiode, filling a blank solution in a detection pool, scanning the energy values, filling filtrate, scanning the energy values again to obtain absorbance, and plotting by taking the diode marks as abscissa and the absorbance as ordinate to obtain an absorption spectrum of an object to be detected; converting the diode mark into an accurate wavelength value, comparing the accurate wavelength value with 451nm, and calibrating the wavelength of the detector when the wavelength is consistent;
the content calculation module is responsible for placing the filtrate in a light path of light with the wavelength of 451nm, calculating to obtain the light absorption value of the filtrate, and converting the light absorption value into the concentration value of carotene in the agricultural products, namely the content value of carotene in the agricultural products.
10. The agricultural product carotene content detection device of claim 9, wherein the content calculation module comprises:
the standard curve establishing module is responsible for measuring the light absorption value of the standard carotene solution under the light with the wavelength of 451nm and establishing a standard curve according to the corresponding relation between the light absorption value and the carotene concentration;
the component concentration calculation module is in charge of transmitting the calculated light absorption value of the filtrate to a processor to be compared with a standard curve, so as to obtain the concentration of carotene in the current agricultural product;
the component content calculating module is responsible for converting the content of carotene in the agricultural products according to the dilution multiple of the filtrate.
CN202310554905.2A 2023-05-16 2023-05-16 Method and device for detecting carotene content of agricultural products Active CN116499832B (en)

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CN102914462A (en) * 2012-11-22 2013-02-06 上海化工研究院 High-throughput method for extracting carotene in biological sample
CN103149165A (en) * 2013-01-28 2013-06-12 西北农林科技大学 Orange cabbage carotenoid extracting and measuring method
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CN111257466A (en) * 2020-02-27 2020-06-09 南宁海关技术中心 Method for measuring carotenoid content in red-yolk duck eggs
CN111812235A (en) * 2020-07-06 2020-10-23 南京中科药业有限公司 Method for measuring content of beta-carotene
CN115792018A (en) * 2022-12-07 2023-03-14 广东省农业科学院作物研究所 Method for determining content of 13 carotenoids in sweet potatoes

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102914462A (en) * 2012-11-22 2013-02-06 上海化工研究院 High-throughput method for extracting carotene in biological sample
CN103149165A (en) * 2013-01-28 2013-06-12 西北农林科技大学 Orange cabbage carotenoid extracting and measuring method
CN105717209A (en) * 2016-01-29 2016-06-29 甘肃农业大学 Method for extracting carotenoid components in pepper leaves and quantitatively detecting content of carotenoid components
CN111257466A (en) * 2020-02-27 2020-06-09 南宁海关技术中心 Method for measuring carotenoid content in red-yolk duck eggs
CN111812235A (en) * 2020-07-06 2020-10-23 南京中科药业有限公司 Method for measuring content of beta-carotene
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