CN116491567A - Preparation method of composite plant extract and application of composite plant extract in heat-clearing rose tea - Google Patents
Preparation method of composite plant extract and application of composite plant extract in heat-clearing rose tea Download PDFInfo
- Publication number
- CN116491567A CN116491567A CN202310186203.3A CN202310186203A CN116491567A CN 116491567 A CN116491567 A CN 116491567A CN 202310186203 A CN202310186203 A CN 202310186203A CN 116491567 A CN116491567 A CN 116491567A
- Authority
- CN
- China
- Prior art keywords
- plant extract
- composite plant
- preparing
- mixing
- composite
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000000419 plant extract Substances 0.000 title claims abstract description 53
- 241000220317 Rosa Species 0.000 title claims abstract description 45
- 239000002131 composite material Substances 0.000 title claims abstract description 45
- 238000002360 preparation method Methods 0.000 title abstract description 30
- 241001122767 Theaceae Species 0.000 title 1
- 244000269722 Thea sinensis Species 0.000 claims abstract description 48
- 239000012535 impurity Substances 0.000 claims abstract description 40
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 33
- 238000000855 fermentation Methods 0.000 claims abstract description 31
- 230000004151 fermentation Effects 0.000 claims abstract description 31
- 244000130270 Fagopyrum tataricum Species 0.000 claims abstract description 27
- 235000014693 Fagopyrum tataricum Nutrition 0.000 claims abstract description 27
- 240000008042 Zea mays Species 0.000 claims abstract description 27
- 235000007244 Zea mays Nutrition 0.000 claims abstract description 26
- 229940089639 cornsilk Drugs 0.000 claims abstract description 26
- 239000001231 zea mays silk Substances 0.000 claims abstract description 26
- 238000000034 method Methods 0.000 claims abstract description 24
- 230000005684 electric field Effects 0.000 claims abstract description 22
- 238000002137 ultrasound extraction Methods 0.000 claims abstract description 12
- 230000008569 process Effects 0.000 claims abstract description 11
- 238000000605 extraction Methods 0.000 claims abstract description 9
- 241000196324 Embryophyta Species 0.000 claims abstract description 8
- 239000007788 liquid Substances 0.000 claims description 60
- 238000002156 mixing Methods 0.000 claims description 42
- 235000013616 tea Nutrition 0.000 claims description 40
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 39
- 241000221198 Basidiomycota Species 0.000 claims description 31
- 230000001580 bacterial effect Effects 0.000 claims description 28
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 24
- 150000001875 compounds Chemical class 0.000 claims description 24
- 238000004108 freeze drying Methods 0.000 claims description 24
- 238000010438 heat treatment Methods 0.000 claims description 19
- 239000000203 mixture Substances 0.000 claims description 19
- 239000007864 aqueous solution Substances 0.000 claims description 18
- 241000186660 Lactobacillus Species 0.000 claims description 17
- 150000001722 carbon compounds Chemical class 0.000 claims description 17
- 229940039696 lactobacillus Drugs 0.000 claims description 17
- 229910021392 nanocarbon Inorganic materials 0.000 claims description 17
- 238000010041 electrostatic spinning Methods 0.000 claims description 16
- 239000002121 nanofiber Substances 0.000 claims description 15
- 230000001105 regulatory effect Effects 0.000 claims description 15
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims description 14
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 14
- 238000012258 culturing Methods 0.000 claims description 14
- 239000001963 growth medium Substances 0.000 claims description 14
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 13
- 238000001816 cooling Methods 0.000 claims description 13
- 239000008103 glucose Substances 0.000 claims description 13
- 239000000843 powder Substances 0.000 claims description 13
- 238000003756 stirring Methods 0.000 claims description 13
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 claims description 12
- 238000011081 inoculation Methods 0.000 claims description 12
- 241000241413 Propolis Species 0.000 claims description 11
- 229940069949 propolis Drugs 0.000 claims description 11
- 239000000243 solution Substances 0.000 claims description 11
- 229920000858 Cyclodextrin Polymers 0.000 claims description 10
- 239000001116 FEMA 4028 Substances 0.000 claims description 10
- 229920001046 Nanocellulose Polymers 0.000 claims description 10
- WHGYBXFWUBPSRW-FOUAGVGXSA-N beta-cyclodextrin Chemical compound OC[C@H]([C@H]([C@@H]([C@H]1O)O)O[C@H]2O[C@@H]([C@@H](O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O3)[C@H](O)[C@H]2O)CO)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@@H]3O[C@@H]1CO WHGYBXFWUBPSRW-FOUAGVGXSA-N 0.000 claims description 10
- 235000011175 beta-cyclodextrine Nutrition 0.000 claims description 10
- 229960004853 betadex Drugs 0.000 claims description 10
- FYGDTMLNYKFZSV-URKRLVJHSA-N (2s,3r,4s,5s,6r)-2-[(2r,4r,5r,6s)-4,5-dihydroxy-2-(hydroxymethyl)-6-[(2r,4r,5r,6s)-4,5,6-trihydroxy-2-(hydroxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1[C@@H](CO)O[C@@H](OC2[C@H](O[C@H](O)[C@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O FYGDTMLNYKFZSV-URKRLVJHSA-N 0.000 claims description 9
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims description 9
- 229920002498 Beta-glucan Polymers 0.000 claims description 9
- 239000000706 filtrate Substances 0.000 claims description 9
- 239000000661 sodium alginate Substances 0.000 claims description 9
- 235000010413 sodium alginate Nutrition 0.000 claims description 9
- 229940005550 sodium alginate Drugs 0.000 claims description 9
- 238000001914 filtration Methods 0.000 claims description 8
- 238000007873 sieving Methods 0.000 claims description 8
- 238000005507 spraying Methods 0.000 claims description 8
- 238000005406 washing Methods 0.000 claims description 8
- 241000251468 Actinopterygii Species 0.000 claims description 7
- 229920001202 Inulin Polymers 0.000 claims description 7
- 239000001888 Peptone Substances 0.000 claims description 7
- 108010080698 Peptones Proteins 0.000 claims description 7
- 229910000019 calcium carbonate Inorganic materials 0.000 claims description 7
- 229940041514 candida albicans extract Drugs 0.000 claims description 7
- JYJIGFIDKWBXDU-MNNPPOADSA-N inulin Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)OC[C@]1(OC[C@]2(OC[C@]3(OC[C@]4(OC[C@]5(OC[C@]6(OC[C@]7(OC[C@]8(OC[C@]9(OC[C@]%10(OC[C@]%11(OC[C@]%12(OC[C@]%13(OC[C@]%14(OC[C@]%15(OC[C@]%16(OC[C@]%17(OC[C@]%18(OC[C@]%19(OC[C@]%20(OC[C@]%21(OC[C@]%22(OC[C@]%23(OC[C@]%24(OC[C@]%25(OC[C@]%26(OC[C@]%27(OC[C@]%28(OC[C@]%29(OC[C@]%30(OC[C@]%31(OC[C@]%32(OC[C@]%33(OC[C@]%34(OC[C@]%35(OC[C@]%36(O[C@@H]%37[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O%37)O)[C@H]([C@H](O)[C@@H](CO)O%36)O)[C@H]([C@H](O)[C@@H](CO)O%35)O)[C@H]([C@H](O)[C@@H](CO)O%34)O)[C@H]([C@H](O)[C@@H](CO)O%33)O)[C@H]([C@H](O)[C@@H](CO)O%32)O)[C@H]([C@H](O)[C@@H](CO)O%31)O)[C@H]([C@H](O)[C@@H](CO)O%30)O)[C@H]([C@H](O)[C@@H](CO)O%29)O)[C@H]([C@H](O)[C@@H](CO)O%28)O)[C@H]([C@H](O)[C@@H](CO)O%27)O)[C@H]([C@H](O)[C@@H](CO)O%26)O)[C@H]([C@H](O)[C@@H](CO)O%25)O)[C@H]([C@H](O)[C@@H](CO)O%24)O)[C@H]([C@H](O)[C@@H](CO)O%23)O)[C@H]([C@H](O)[C@@H](CO)O%22)O)[C@H]([C@H](O)[C@@H](CO)O%21)O)[C@H]([C@H](O)[C@@H](CO)O%20)O)[C@H]([C@H](O)[C@@H](CO)O%19)O)[C@H]([C@H](O)[C@@H](CO)O%18)O)[C@H]([C@H](O)[C@@H](CO)O%17)O)[C@H]([C@H](O)[C@@H](CO)O%16)O)[C@H]([C@H](O)[C@@H](CO)O%15)O)[C@H]([C@H](O)[C@@H](CO)O%14)O)[C@H]([C@H](O)[C@@H](CO)O%13)O)[C@H]([C@H](O)[C@@H](CO)O%12)O)[C@H]([C@H](O)[C@@H](CO)O%11)O)[C@H]([C@H](O)[C@@H](CO)O%10)O)[C@H]([C@H](O)[C@@H](CO)O9)O)[C@H]([C@H](O)[C@@H](CO)O8)O)[C@H]([C@H](O)[C@@H](CO)O7)O)[C@H]([C@H](O)[C@@H](CO)O6)O)[C@H]([C@H](O)[C@@H](CO)O5)O)[C@H]([C@H](O)[C@@H](CO)O4)O)[C@H]([C@H](O)[C@@H](CO)O3)O)[C@H]([C@H](O)[C@@H](CO)O2)O)[C@@H](O)[C@H](O)[C@@H](CO)O1 JYJIGFIDKWBXDU-MNNPPOADSA-N 0.000 claims description 7
- 229940029339 inulin Drugs 0.000 claims description 7
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 7
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 7
- 238000004519 manufacturing process Methods 0.000 claims description 7
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 7
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 7
- 229910000403 monosodium phosphate Inorganic materials 0.000 claims description 7
- 235000019799 monosodium phosphate Nutrition 0.000 claims description 7
- 235000019319 peptone Nutrition 0.000 claims description 7
- 235000020183 skimmed milk Nutrition 0.000 claims description 7
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 claims description 7
- 235000019832 sodium triphosphate Nutrition 0.000 claims description 7
- 239000002904 solvent Substances 0.000 claims description 7
- 238000009210 therapy by ultrasound Methods 0.000 claims description 7
- 239000012138 yeast extract Substances 0.000 claims description 7
- 240000002605 Lactobacillus helveticus Species 0.000 claims description 6
- 235000013967 Lactobacillus helveticus Nutrition 0.000 claims description 6
- 235000019270 ammonium chloride Nutrition 0.000 claims description 6
- 239000012300 argon atmosphere Substances 0.000 claims description 6
- 239000012298 atmosphere Substances 0.000 claims description 6
- 229940054346 lactobacillus helveticus Drugs 0.000 claims description 6
- 230000001590 oxidative effect Effects 0.000 claims description 6
- 235000006468 Thea sinensis Nutrition 0.000 claims description 5
- 238000010025 steaming Methods 0.000 claims description 5
- 235000020279 black tea Nutrition 0.000 claims description 4
- 241000628997 Flos Species 0.000 claims description 3
- 241001468157 Lactobacillus johnsonii Species 0.000 claims description 3
- 238000004806 packaging method and process Methods 0.000 claims description 2
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 2
- 238000001035 drying Methods 0.000 claims 1
- 238000000227 grinding Methods 0.000 claims 1
- 238000005516 engineering process Methods 0.000 abstract description 12
- 238000002834 transmittance Methods 0.000 abstract description 11
- 239000004480 active ingredient Substances 0.000 abstract description 8
- 238000012545 processing Methods 0.000 abstract description 4
- 238000007710 freezing Methods 0.000 abstract description 3
- 230000008014 freezing Effects 0.000 abstract description 3
- 230000036772 blood pressure Effects 0.000 description 16
- 240000000599 Lentinula edodes Species 0.000 description 15
- 230000000052 comparative effect Effects 0.000 description 13
- 239000000126 substance Substances 0.000 description 12
- 238000012360 testing method Methods 0.000 description 12
- 235000001715 Lentinula edodes Nutrition 0.000 description 10
- BTCSSZJGUNDROE-UHFFFAOYSA-N gamma-aminobutyric acid Chemical compound NCCCC(O)=O BTCSSZJGUNDROE-UHFFFAOYSA-N 0.000 description 10
- 230000001737 promoting effect Effects 0.000 description 9
- 230000001603 reducing effect Effects 0.000 description 8
- 241000700159 Rattus Species 0.000 description 7
- 239000008280 blood Substances 0.000 description 7
- 210000004369 blood Anatomy 0.000 description 7
- 241000894006 Bacteria Species 0.000 description 6
- 230000000694 effects Effects 0.000 description 6
- 239000003205 fragrance Substances 0.000 description 6
- OGNSCSPNOLGXSM-UHFFFAOYSA-N (+/-)-DABA Natural products NCCC(N)C(O)=O OGNSCSPNOLGXSM-UHFFFAOYSA-N 0.000 description 5
- 241000186673 Lactobacillus delbrueckii Species 0.000 description 5
- 230000004531 blood pressure lowering effect Effects 0.000 description 5
- 239000003814 drug Substances 0.000 description 5
- 229960003692 gamma aminobutyric acid Drugs 0.000 description 5
- 230000002401 inhibitory effect Effects 0.000 description 5
- 239000002054 inoculum Substances 0.000 description 5
- 210000004185 liver Anatomy 0.000 description 5
- 235000013824 polyphenols Nutrition 0.000 description 5
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 5
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 5
- 239000013543 active substance Substances 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 4
- 239000000284 extract Substances 0.000 description 4
- 239000002932 luster Substances 0.000 description 4
- 239000000049 pigment Substances 0.000 description 4
- 150000008442 polyphenolic compounds Chemical class 0.000 description 4
- 239000002994 raw material Substances 0.000 description 4
- 235000017166 Bambusa arundinacea Nutrition 0.000 description 3
- 235000017491 Bambusa tulda Nutrition 0.000 description 3
- 244000082204 Phyllostachys viridis Species 0.000 description 3
- 235000015334 Phyllostachys viridis Nutrition 0.000 description 3
- 241000700157 Rattus norvegicus Species 0.000 description 3
- 239000011425 bamboo Substances 0.000 description 3
- 230000017531 blood circulation Effects 0.000 description 3
- 230000037396 body weight Effects 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 230000004060 metabolic process Effects 0.000 description 3
- 206010065559 Cerebral arteriosclerosis Diseases 0.000 description 2
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 2
- 208000002193 Pain Diseases 0.000 description 2
- 208000004880 Polyuria Diseases 0.000 description 2
- 230000003213 activating effect Effects 0.000 description 2
- 230000001773 anti-convulsant effect Effects 0.000 description 2
- 239000001961 anticonvulsive agent Substances 0.000 description 2
- 229960003965 antiepileptics Drugs 0.000 description 2
- 210000003169 central nervous system Anatomy 0.000 description 2
- 230000035619 diuresis Effects 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 238000001704 evaporation Methods 0.000 description 2
- 229930003935 flavonoid Natural products 0.000 description 2
- 150000002215 flavonoids Chemical class 0.000 description 2
- 235000017173 flavonoids Nutrition 0.000 description 2
- 235000013922 glutamic acid Nutrition 0.000 description 2
- 239000004220 glutamic acid Substances 0.000 description 2
- 150000004676 glycans Chemical class 0.000 description 2
- 229910001385 heavy metal Inorganic materials 0.000 description 2
- 201000005851 intracranial arteriosclerosis Diseases 0.000 description 2
- 230000027939 micturition Effects 0.000 description 2
- 239000002858 neurotransmitter agent Substances 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000036407 pain Effects 0.000 description 2
- 238000009928 pasteurization Methods 0.000 description 2
- 229920001282 polysaccharide Polymers 0.000 description 2
- 239000005017 polysaccharide Substances 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 235000018102 proteins Nutrition 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 210000000952 spleen Anatomy 0.000 description 2
- 210000002784 stomach Anatomy 0.000 description 2
- 230000008961 swelling Effects 0.000 description 2
- 230000001988 toxicity Effects 0.000 description 2
- 231100000419 toxicity Toxicity 0.000 description 2
- 230000003442 weekly effect Effects 0.000 description 2
- TUSDEZXZIZRFGC-UHFFFAOYSA-N 1-O-galloyl-3,6-(R)-HHDP-beta-D-glucose Natural products OC1C(O2)COC(=O)C3=CC(O)=C(O)C(O)=C3C3=C(O)C(O)=C(O)C=C3C(=O)OC1C(O)C2OC(=O)C1=CC(O)=C(O)C(O)=C1 TUSDEZXZIZRFGC-UHFFFAOYSA-N 0.000 description 1
- FPIPGXGPPPQFEQ-UHFFFAOYSA-N 13-cis retinol Natural products OCC=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-UHFFFAOYSA-N 0.000 description 1
- 208000002874 Acne Vulgaris Diseases 0.000 description 1
- 206010003210 Arteriosclerosis Diseases 0.000 description 1
- 208000006820 Arthralgia Diseases 0.000 description 1
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Natural products OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 1
- 206010003497 Asphyxia Diseases 0.000 description 1
- 241000223678 Aureobasidium pullulans Species 0.000 description 1
- 206010006298 Breast pain Diseases 0.000 description 1
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- 235000005979 Citrus limon Nutrition 0.000 description 1
- 244000131522 Citrus pyriformis Species 0.000 description 1
- 208000009084 Cold Injury Diseases 0.000 description 1
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 1
- 244000169544 Duchesnea indica Species 0.000 description 1
- 239000001263 FEMA 3042 Substances 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- 206010020772 Hypertension Diseases 0.000 description 1
- 208000006662 Mastodynia Diseases 0.000 description 1
- 235000017784 Mespilus germanica Nutrition 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 244000182216 Mimusops elengi Species 0.000 description 1
- 235000000560 Mimusops elengi Nutrition 0.000 description 1
- LRBQNJMCXXYXIU-PPKXGCFTSA-N Penta-digallate-beta-D-glucose Natural products OC1=C(O)C(O)=CC(C(=O)OC=2C(=C(O)C=C(C=2)C(=O)OC[C@@H]2[C@H]([C@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)O2)OC(=O)C=2C=C(OC(=O)C=3C=C(O)C(O)=C(O)C=3)C(O)=C(O)C=2)O)=C1 LRBQNJMCXXYXIU-PPKXGCFTSA-N 0.000 description 1
- 241000209504 Poaceae Species 0.000 description 1
- 241000219050 Polygonaceae Species 0.000 description 1
- 241000109329 Rosa xanthina Species 0.000 description 1
- 235000004789 Rosa xanthina Nutrition 0.000 description 1
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 description 1
- 235000007837 Vangueria infausta Nutrition 0.000 description 1
- FPIPGXGPPPQFEQ-BOOMUCAASA-N Vitamin A Natural products OC/C=C(/C)\C=C\C=C(\C)/C=C/C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-BOOMUCAASA-N 0.000 description 1
- 229930003270 Vitamin B Natural products 0.000 description 1
- 229930003268 Vitamin C Natural products 0.000 description 1
- 229930003427 Vitamin E Natural products 0.000 description 1
- 229930003448 Vitamin K Natural products 0.000 description 1
- 206010000496 acne Diseases 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 229930013930 alkaloid Natural products 0.000 description 1
- 150000003797 alkaloid derivatives Chemical class 0.000 description 1
- FPIPGXGPPPQFEQ-OVSJKPMPSA-N all-trans-retinol Chemical compound OC\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-OVSJKPMPSA-N 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 208000011775 arteriosclerosis disease Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 235000021028 berry Nutrition 0.000 description 1
- 230000000740 bleeding effect Effects 0.000 description 1
- 238000009530 blood pressure measurement Methods 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 230000002490 cerebral effect Effects 0.000 description 1
- 208000026106 cerebrovascular disease Diseases 0.000 description 1
- 230000004087 circulation Effects 0.000 description 1
- 230000002595 cold damage Effects 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- GVJHHUAWPYXKBD-UHFFFAOYSA-N d-alpha-tocopherol Natural products OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 230000002526 effect on cardiovascular system Effects 0.000 description 1
- 230000002124 endocrine Effects 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 229930003944 flavone Natural products 0.000 description 1
- 150000002212 flavone derivatives Chemical class 0.000 description 1
- 235000011949 flavones Nutrition 0.000 description 1
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 description 1
- 230000002496 gastric effect Effects 0.000 description 1
- 235000009569 green tea Nutrition 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 230000005906 menstruation Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 210000005036 nerve Anatomy 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 235000020333 oolong tea Nutrition 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000001814 pectin Substances 0.000 description 1
- 229920001277 pectin Polymers 0.000 description 1
- 235000010987 pectin Nutrition 0.000 description 1
- SHUZOJHMOBOZST-UHFFFAOYSA-N phylloquinone Natural products CC(C)CCCCC(C)CCC(C)CCCC(=CCC1=C(C)C(=O)c2ccccc2C1=O)C SHUZOJHMOBOZST-UHFFFAOYSA-N 0.000 description 1
- -1 polyphenol peroxide Chemical class 0.000 description 1
- 208000020016 psychiatric disease Diseases 0.000 description 1
- 239000001397 quillaja saponaria molina bark Substances 0.000 description 1
- 239000012088 reference solution Substances 0.000 description 1
- 229930182490 saponin Natural products 0.000 description 1
- 150000007949 saponins Chemical class 0.000 description 1
- 210000000582 semen Anatomy 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 230000035488 systolic blood pressure Effects 0.000 description 1
- 235000015523 tannic acid Nutrition 0.000 description 1
- 229920002258 tannic acid Polymers 0.000 description 1
- LRBQNJMCXXYXIU-NRMVVENXSA-N tannic acid Chemical compound OC1=C(O)C(O)=CC(C(=O)OC=2C(=C(O)C=C(C=2)C(=O)OC[C@@H]2[C@H]([C@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)O2)OC(=O)C=2C=C(OC(=O)C=3C=C(O)C(O)=C(O)C=3)C(O)=C(O)C=2)O)=C1 LRBQNJMCXXYXIU-NRMVVENXSA-N 0.000 description 1
- 229940033123 tannic acid Drugs 0.000 description 1
- 235000018553 tannin Nutrition 0.000 description 1
- 229920001864 tannin Polymers 0.000 description 1
- 239000001648 tannin Substances 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 230000008736 traumatic injury Effects 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 235000019155 vitamin A Nutrition 0.000 description 1
- 239000011719 vitamin A Substances 0.000 description 1
- 235000019156 vitamin B Nutrition 0.000 description 1
- 239000011720 vitamin B Substances 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
- 239000011718 vitamin C Substances 0.000 description 1
- 235000019165 vitamin E Nutrition 0.000 description 1
- 239000011709 vitamin E Substances 0.000 description 1
- 235000019168 vitamin K Nutrition 0.000 description 1
- 239000011712 vitamin K Substances 0.000 description 1
- 229940045997 vitamin a Drugs 0.000 description 1
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 description 1
- 239000000341 volatile oil Substances 0.000 description 1
- 230000029663 wound healing Effects 0.000 description 1
- 230000037303 wrinkles Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/34—Tea substitutes, e.g. matè; Extracts or infusions thereof
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a preparation method of a composite plant extract and application thereof in heat-clearing rose tea, wherein the composite plant is corn silk and tartary buckwheat seeds, the corn silk and the tartary buckwheat seeds are subjected to low-temperature rapid freezing treatment, immediately crushed and then combined with a high-voltage pulse electric field technology, an ultrasonic extraction technology and a low-temperature fermentation technology, and the whole process is extracted at low temperature, so that loss of active ingredients caused by high-temperature processing is avoided, and meanwhile, the extraction rate of the active ingredients is also improved; by adding the impurity removing agent, the extracting solution can be well purified and impurity removed, the light transmittance of the rose tea can be effectively improved, and the influence of impurities on the color and taste of the rose tea can be avoided.
Description
Technical Field
The invention relates to the technical field of plant extraction, in particular to a preparation method of a compound plant extract and application of the compound plant extract in heat-clearing rose tea.
Background
Roses are woody plants which integrate medical, edible, beautifying and greening functions. The rose flower is warm in nature, sweet in taste and slightly bitter, enters liver and spleen meridians, is a good medicine for treating gynecopathy, cardiovascular and cerebrovascular diseases, traumatic injury and mental diseases, and the use of rose flower buds as a medicine has been known for hundreds of years. "Ben Cao Zhengyi" carries: the rose has the advantages of being the most intense in fragrance, clear and not turbid, and not strong, softening liver and restoring stomach, regulating qi and activating blood, and dispersing and relieving asphyxia, and no Xin Wengang dryness is the most effective and most domesticated among the medicines by breaking and pushing qi and the medicines, and the fragrance is the most excellent, so that the Chinese medicinal composition has no match. "Ben Cao Shi Yi" on: "activating blood and promoting blood circulation, regulating qi-flowing, treating wind arthralgia, mastalgia, early stage of liver swelling and toxicity, and pain of liver and stomach qi". "
The scented tea is unique processed tea in China, each scented tea contains more or less natural nutritional ingredients, the scented tea is mainly prepared by taking green tea, black tea or oolong tea as tea blanks and fresh flowers capable of spitting fragrance as raw materials, and a scenting process is adopted. The flos Rosae Rugosae is rich in vitamin A, C, B, E, K and tannin, and can improve endocrine disturbance, and is helpful for relieving fatigue and wound healing. Regulating qi and blood, regulating female physiological problems, promoting blood circulation, caring skin, regulating menstruation, promoting urination, relieving gastrointestinal nerves, preventing wrinkle, preventing cold injury, caring skin, clearing heat and toxic materials, relieving swelling, eliminating acne, and clearing liver fire. The rose tea is a high-grade tea which is prepared by mixing fresh rose flowers and bud tips of tea leaves in proportion and scenting by using a modern high-tech technology, has strong fragrance and light weight, and is not violent, so that more people drink the rose tea at present. However, the rose tea has the defect of single nutrition component and function when being drunk independently.
CN105454583a discloses a rose tea and a preparation method thereof, the weight ratio of the raw material components is: 100-200 parts of fresh rose, 20-30 parts of bamboo leaves, 10-12 parts of lemon, 1-3 parts of salt, 1-3 parts of bamboo shoots, 3-5 parts of mock-strawberry and 2-4 parts of medlar. The rose tea prepared by the invention has the advantages of strong fragrance and bright color, and the rose tea is soaked in the nutrient solution prepared from the bamboo shoots, the mock berries and the like, so that the rose tea has the health care effects of clearing heat, detoxicating and the like. The rose tea has more raw materials, is not extracted, and has more impurities in the soaking process to influence the color and taste of the rose tea.
Disclosure of Invention
In view of the above-mentioned drawbacks of the prior art, the technical problem to be solved by the present invention is to provide a heat-clearing rose tea with good glossiness, fragrance, taste and blood pressure reducing effect.
In order to achieve the aim, the composite plant extract is added into the rose tea, the composite plant is corn silk and tartary buckwheat seeds with the functions of clearing heat and removing toxicity, the whole extraction process is low-temperature extraction, the loss of active ingredients caused by high-temperature processing is avoided, the fermentation is performed by adopting artificial inoculation dominant bacteria, the blood pressure reducing effect of the corn silk and the tartary buckwheat seeds is improved, the impurity removing agent is added, the extracting solution can be well purified and removed, the light transmittance of the rose tea is effectively improved, the influence of the existence of impurities on the color and taste of the rose tea is avoided, and the blood pressure reducing function of the rose tea is endowed.
In order to achieve the above object, the present invention adopts the following technical scheme:
a preparation method of a compound plant extract comprises the following steps:
mixing corn silk and tartary buckwheat seeds, washing with water, spraying a beta-cyclodextrin aqueous solution with the concentration of 0.5-1.5wt% on the surface, standing for 10-12h, freeze-drying at-30-20 ℃ for 1-2h, immediately crushing after freeze-drying, sieving with a 80-100 mesh sieve, adding water according to the feed liquid ratio of 1:10-15g/mL, and performing ultrasonic extraction for 1-2h at room temperature, 60-100Hz and 120-150W; extracting under high-voltage pulse electric field for 10-30min, regulating pH to 5-7 with 30-36wt% acetic acid aqueous solution to obtain mixture, adding mixed bacterial liquid into the mixture, fermenting at 25-30deg.C for 8-10d, adding impurity removing agent into the fermentation broth after fermentation, stirring for 10-20min, filtering, steaming filtrate under reduced pressure to remove solvent, pasteurizing at 60-80deg.C for 20-30min, and freeze drying at-30 to-20deg.C for 6-12 hr to obtain composite plant extract.
Preferably, the dosage ratio of the corn silk to the tartary buckwheat seeds to the beta-cyclodextrin aqueous solution is 500-1000:500-1000:5-10.
Preferably, the high-voltage pulse electric field extraction condition is that the electric field intensity is 20-40kV/cm, the pulse time is 400-500 mu s, and the pulse frequency is 200-300Hz.
Preferably, the mass ratio of the mixed bacterial liquid is 1-2: mixing 2-3 of Lactobacillus delbrueckii bacteria liquid with Basidiomycetes liquid; the addition amount of the mixed bacterial liquid is 2-5% of the mass of the mixture.
Preferably, the preparation method of the lactobacillus johnsonii bacterial liquid comprises the following steps:
inoculating Lactobacillus helveticus to a culture medium prepared by mixing 2-5wt% glucose, 2-3wt% fish peptone, 1-2wt% yeast extract, 0.1-0.5wt% potassium dihydrogen phosphate, 0.1-0.5wt% calcium carbonate and the balance water, wherein the inoculation amount is 1-3wt%, and culturing at 25-35deg.C for 24-36 hr to obtain Lactobacillus helveticus bacterial liquid.
Preferably, the preparation method of the Basidiomycetes liquid comprises the following steps:
inoculating Basidiomycetes in a culture medium prepared by uniformly mixing 6-10wt% of skimmed milk powder, 2-5wt% of inulin, 0.5-1.2wt% of sodium dihydrogen phosphate, 0.1-1.0wt% of magnesium sulfate and the balance of water, wherein the inoculation amount is 1-2wt%, and culturing at 25-35deg.C for 24-36 hr to obtain Basidiomycetes liquid.
Corn silk is the colume and stigma of corn Zea mays L. Of Gramineae, contains various chemical components such as saponin, volatile oil, fatty acid, alkaloid, flavonoids, polysaccharide, vitamins and minerals, has mild taste, and has effects of promoting urination, lowering blood sugar, inhibiting bacteria, lowering blood pressure, enhancing immunity, clearing heat and detoxicating, promoting diuresis, treating stranguria, and stopping bleeding; anticancer effect; tartary buckwheat, namely, fagopyrum tataricum, is a plant of Polygonaceae, and its seeds can be eaten or used as feed; the root can be used for medicine, has the effects of regulating qi, relieving pain, strengthening spleen, promoting diuresis, relieving arteriosclerosis, improving circulation, clearing heat, detoxicating, promoting blood circulation, removing blood stasis, invigorating qi, refreshing, and regulating blood pressure.
According to the invention, corn silk and tartary buckwheat seeds are subjected to low-temperature rapid freezing treatment, immediately crushed, and then combined with a high-voltage pulse electric field technology, an ultrasonic extraction technology and a low-temperature fermentation technology, so that the loss of active ingredients caused by high-temperature processing is avoided, the extraction rate of the active ingredients is improved, and the inventor adopts artificial inoculation dominant bacteria for fermentation, so that the blood pressure reducing effect of the corn silk and tartary buckwheat seeds is improved. The corn silk and the tartary buckwheat seeds contain a large amount of active substances such as polysaccharide, flavone, polyphenol, amino acid, protein organic acid and the like, after the active substances are extracted by a high-voltage pulse electric field technology and an ultrasonic extraction technology, the active substances can effectively promote the growth and mass propagation of the active substances under the action of mixed bacterial liquid, more blood pressure-reducing flavonoid substances can be generated in the fermentation process of the corn silk and the tartary buckwheat seeds, the blood pressure-reducing effect of the composite plant extract is further improved, the use value of the composite plant extract is improved, and after the fermentation, the active ingredient molecules contained in the extract are smaller, have better affinity and can be better absorbed by human bodies. Meanwhile, the Lactobacillus delbrueckii and the Basidiomycetes can promote the glutamic acid in corn silk and tartary buckwheat seeds to be converted into gamma-aminobutyric acid, and the gamma-aminobutyric acid is an important inhibitory neurotransmitter in the central nervous system, has the effects of anticonvulsant, promoting brain metabolism, preventing cerebral arteriosclerosis and obviously inhibiting blood pressure rise, and improves the blood pressure reducing effect of the compound plant extract.
Preferably, the adding amount of the impurity removing agent is 1-3 per mill of the mass of the fermentation liquor.
Preferably, the preparation method of the impurity removing agent comprises the following steps:
1) Mixing 100-120g of glucose and 100-120g of ammonium chloride in an argon atmosphere, placing in a tube furnace, heating to 800-900 ℃ at a heating rate of 200-250 ℃/min, reacting at 800-900 ℃ for 3-5h, cooling to room temperature to obtain a bubble, and oxidizing the bubble in an air atmosphere at 300-400 ℃ for 6-8h to obtain a nano carbon compound;
2) Adding 10-15g of nano carbon compound, 15-20g of nano cellulose, 20-25g of sodium alginate and 10-15g of beta-glucan into 50-80g of water, carrying out ultrasonic treatment for 20-30min under the conditions of 50-80Hz and 1500-2000W, adding 1-3g of sodium tripolyphosphate, heating to 80-100 ℃, reacting for 2-4h, cooling to room temperature, adding 30-50g of propolis, stirring and mixing at the room temperature at the rotation speed of 600-800rpm for 1-2h, and obtaining a mixed system;
3) And (3) carrying out electrostatic spinning on the mixed system obtained in the step (2), applying 15-20KV high voltage in the electrostatic spinning process, enabling the distance between a nozzle of an injector and a receiving device to be 10-15cm, obtaining composite nano fibers, and then manufacturing the composite nano fibers into a sheet-shaped structure to obtain the impurity removing agent.
The invention also discloses application of the composite plant extract in heat-clearing rose tea, which is specifically applied as follows: fresh rose and plants with heat clearing function commonly used in the field are dried, mixed with the composite plant extract and black tea, and ground into powder for packaging.
Preferably, the plant with heat clearing function is one or more of semen Cassiae, galla chinensis, flos Lonicerae and folium Mori.
The composite plant extract contains a small amount of tannic acid, protein, pectin, inorganic salts and pigment, so that the light transmittance of the composite plant extract is poor, the color and luster of the rose tea can be influenced, the rich product produced by fermentation and metabolism of the microbial agent can destroy the balance of a rose tea system, the inventor loads nano fibers through nano carbon compounds, a porous three-wall network structure with high hole rate and high specific surface area is formed through electrostatic spinning, the extract can be well purified and decontaminated, the light transmittance of the rose tea is effectively improved, the color and luster of the rose tea are prevented from being influenced by the existence of impurities, the contact area with impurities and macromolecular substances is increased through electrostatic spinning, the porous structure of the nano carbon compounds can adsorb heavy metals and other bacteria in the plant extract, sodium alginate and beta-glucan can promote the propolis to form a composite film to be attached to the surface of materials formed by the nano carbon compounds, nano cellulose and the like, the active group on the surface of the propolis can be purified through the composite plant extract system, the active group and the bitter and overoxidation polyphenol in the composite plant extract can react, so that the overoxidation polyphenol and unstable pigment substances in the extract can be effectively removed, and the rose flower has the characteristics of good luster, mellow taste and luster.
Compared with the prior art, the invention has the beneficial effects that: the invention firstly carries out low-temperature quick freezing treatment on corn silk and tartary buckwheat seeds, immediately crushes the corn silk and tartary buckwheat seeds, combines the corn silk and tartary buckwheat seeds with a high-voltage pulse electric field technology, an ultrasonic extraction technology and a low-temperature fermentation technology, and carries out low-temperature extraction in the whole process, thereby avoiding the loss of active ingredients caused by high-temperature processing and improving the extraction rate of the active ingredients; the fermentation is carried out by adopting artificial inoculation dominant bacteria, so that the blood pressure reducing effect of corn silk and tartary buckwheat seeds is improved; the extracting solution is treated by the impurity removing agent, so that the extracting solution can be well purified and impurity removed, the light transmittance of the rose tea can be effectively improved, and the blood pressure reducing effect of the rose tea can be improved.
Detailed Description
For the sake of brevity, the articles used in the examples below are commercially available products unless otherwise specified, and the methods used are conventional methods unless otherwise specified.
The sources of part of raw materials used in the invention are as follows:
corn silk, the origin Hubei.
Tartary buckwheat seeds, producing land Ningxia
Lactobacillus shibateri, lentilobacillus, CICC25208, available from the China center for type culture Collection of microorganisms.
Basidiomycetes, latin brand Aureobasidium pullulans, CICC40320, were purchased from the China industry microbiological culture Collection center.
Propolis, concentrated powder, 100 mesh, six-plate rhyme biotechnology company of chicken.
The content of nanocellulose is 99.5%, the particle size is 20-50nm, and the WUHan dynasty Hua Xiangke biological technology Co., ltd.
Example 1
A preparation method of a compound plant extract comprises the following steps:
mixing 500g corn silk and 500g tartary buckwheat seeds, washing with water, spraying 5g of 1.0wt% beta-cyclodextrin aqueous solution on the surface, standing for 12h, freeze-drying at-20 ℃ for 2h, immediately crushing after freeze-drying, sieving with a 80-mesh sieve, adding water according to a feed-liquid ratio of 1:15g/mL, and carrying out ultrasonic extraction for 2h at room temperature under the conditions of 80Hz and 120W; extracting under high-voltage pulse electric field with electric field intensity of 30kV/cm, pulse time of 500 mus and pulse frequency of 300Hz for 20min, regulating pH to 6 with 36wt% acetic acid aqueous solution to obtain mixture, adding mixed bacterial liquid prepared by mixing Lactobacillus delbrueckii bacterial liquid and Basidiomycetes bacterial liquid with mass ratio of 1:2 into the mixture, fermenting at 30deg.C for 10d, adding impurity removing agent into the fermentation liquid after fermentation, adding impurity removing agent in an amount of 5 per mill of the mass of the fermentation liquid, stirring for 10min, filtering, steaming the filtrate under reduced pressure to remove solvent, pasteurizing at 70deg.C for 20min, and freeze drying at-20deg.C for 12h to obtain compound plant extract.
The preparation method of the lactobacillus shiitake fungus solution comprises the following steps:
lactobacillus shiitake is inoculated on a culture medium which is formed by mixing 3 weight percent of glucose, 2.5 weight percent of fish peptone, 1.5 weight percent of yeast extract, 0.3 weight percent of monopotassium phosphate, 0.2 weight percent of calcium carbonate and the balance of water, the inoculation amount is 2 weight percent, and the lactobacillus shiitake bacterial liquid is obtained by culturing for 36 hours at the temperature of 30 ℃.
The preparation method of the basidiomycete liquid comprises the following steps:
inoculating the Basidiomycetes in a culture medium prepared by uniformly mixing 8wt% of skimmed milk powder, 3wt% of inulin, 0.8wt% of sodium dihydrogen phosphate, 0.5wt% of magnesium sulfate and the balance of water, wherein the inoculum size is 1.5wt%, and culturing for 30 hours at 30 ℃ to obtain the Basidiomycetes liquid.
The preparation method of the impurity removing agent comprises the following steps:
1) Under argon atmosphere, mixing 100g of glucose with 100g of ammonium chloride, placing in a tube furnace, heating to 900 ℃ at a heating rate of 250 ℃/min, then reacting at 900 ℃ for 3 hours, cooling to room temperature to obtain a bubble-like substance, and then placing the bubble-like substance in air atmosphere and oxidizing at 400 ℃ for 8 hours to obtain a nano carbon compound;
2) Adding 12.5g of nano carbon compound, 18g of nano cellulose, 23g of sodium alginate and 12.8g of beta-glucan into 80g of water, carrying out ultrasonic treatment for 20min under the conditions of 60Hz and 1800W, adding 2.5g of sodium tripolyphosphate, heating to 100 ℃, cooling to room temperature after reaction for 3 hours, adding 40g of propolis, and stirring and mixing at the room temperature at the rotating speed of 700rpm for 2 hours to obtain a mixed system;
3) And 2) carrying out electrostatic spinning on the mixed system obtained in the step 2), applying 18KV high voltage in the electrostatic spinning process, enabling the distance between a nozzle of an injector and a receiving device to be 12cm, obtaining composite nano fibers, and then manufacturing the composite nano fibers into a sheet-shaped structure to obtain the impurity removing agent.
Comparative example 1
A preparation method of a compound plant extract comprises the following steps:
mixing 500g corn silk and 500g tartary buckwheat seeds, washing with water, spraying 5g of 1.0wt% beta-cyclodextrin aqueous solution on the surface, standing for 12h, freeze-drying at-20 ℃ for 2h, immediately crushing after freeze-drying, sieving with a 80-mesh sieve, adding water according to a feed-liquid ratio of 1:15g/mL, and carrying out ultrasonic extraction for 2h at room temperature under the conditions of 80Hz and 120W; extracting for 20min under a high-voltage pulse electric field with the electric field intensity of 30kV/cm and the pulse time of 500 mus and the pulse frequency of 300Hz, regulating the pH value to 6 by using 36wt% acetic acid aqueous solution to obtain a mixture, adding lactobacillus johnsonii bacterial liquid with the addition of 3 percent of the mass of the mixture into the mixture, fermenting for 10d at 30 ℃, adding an impurity removing agent into the fermentation broth after the fermentation is completed, wherein the addition of the impurity removing agent is 5 per mill of the mass of the fermentation broth, filtering after stirring for 10min, carrying out reduced pressure evaporation on the filtrate to remove the solvent, carrying out pasteurization at the temperature of 70 ℃ for 20min, and finally carrying out freeze drying at the temperature of 20 ℃ below zero for 12h to obtain the composite plant extract.
The preparation method of the lactobacillus shiitake fungus solution comprises the following steps:
lactobacillus shiitake is inoculated on a culture medium which is formed by mixing 3 weight percent of glucose, 2.5 weight percent of fish peptone, 1.5 weight percent of yeast extract, 0.3 weight percent of monopotassium phosphate, 0.2 weight percent of calcium carbonate and the balance of water, the inoculation amount is 2 weight percent, and the lactobacillus shiitake bacterial liquid is obtained by culturing for 36 hours at the temperature of 30 ℃.
The preparation method of the impurity removing agent comprises the following steps:
1) Under argon atmosphere, mixing 100g of glucose with 100g of ammonium chloride, placing in a tube furnace, heating to 900 ℃ at a heating rate of 250 ℃/min, then reacting at 900 ℃ for 3 hours, cooling to room temperature to obtain a bubble-like substance, and then placing the bubble-like substance in air atmosphere and oxidizing at 400 ℃ for 8 hours to obtain a nano carbon compound;
2) Adding 12.5g of nano carbon compound, 18g of nano cellulose, 23g of sodium alginate and 12.8g of beta-glucan into 80g of water, carrying out ultrasonic treatment for 20min under the conditions of 60Hz and 1800W, adding 2.5g of sodium tripolyphosphate, heating to 100 ℃, cooling to room temperature after reaction for 3 hours, adding 40g of propolis, and stirring and mixing at the room temperature at the rotating speed of 700rpm for 2 hours to obtain a mixed system;
3) And 2) carrying out electrostatic spinning on the mixed system obtained in the step 2), applying 18KV high voltage in the electrostatic spinning process, enabling the distance between a nozzle of an injector and a receiving device to be 12cm, obtaining composite nano fibers, and then manufacturing the composite nano fibers into a sheet-shaped structure to obtain the impurity removing agent.
Comparative example 2
A preparation method of a compound plant extract comprises the following steps:
mixing 500g corn silk and 500g tartary buckwheat seeds, washing with water, spraying 5g of 1.0wt% beta-cyclodextrin aqueous solution on the surface, standing for 12h, freeze-drying at-20 ℃ for 2h, immediately crushing after freeze-drying, sieving with a 80-mesh sieve, adding water according to a feed-liquid ratio of 1:15g/mL, and carrying out ultrasonic extraction for 2h at room temperature under the conditions of 80Hz and 120W; extracting for 20min under a high-voltage pulse electric field with the electric field intensity of 30kV/cm, the pulse time of 500 mu s and the pulse frequency of 300Hz, regulating the pH value to 6 by using 36wt% acetic acid aqueous solution to obtain a mixture, adding the basidiomycete liquid with the addition of 3 percent of the mass of the mixture into the mixture, fermenting for 10d at the temperature of 30 ℃, adding an impurity removing agent into the fermentation broth after the fermentation is completed, wherein the addition of the impurity removing agent is 5 per mill of the mass of the fermentation broth, filtering after stirring for 10min, carrying out pasteurization on the filtrate at the temperature of 70 ℃ for 20min after the filtrate is decompressed and distilled, and finally carrying out freeze drying for 12h at the temperature of minus 20 ℃ to obtain the composite plant extract.
The preparation method of the basidiomycete liquid comprises the following steps:
inoculating the Basidiomycetes in a culture medium prepared by uniformly mixing 8wt% of skimmed milk powder, 3wt% of inulin, 0.8wt% of sodium dihydrogen phosphate, 0.5wt% of magnesium sulfate and the balance of water, wherein the inoculum size is 1.5wt%, and culturing for 30 hours at 30 ℃ to obtain the Basidiomycetes liquid.
The preparation method of the impurity removing agent comprises the following steps:
1) Under argon atmosphere, mixing 100g of glucose with 100g of ammonium chloride, placing in a tube furnace, heating to 900 ℃ at a heating rate of 250 ℃/min, then reacting at 900 ℃ for 3 hours, cooling to room temperature to obtain a bubble-like substance, and then placing the bubble-like substance in air atmosphere and oxidizing at 400 ℃ for 8 hours to obtain a nano carbon compound;
2) Adding 12.5g of nano carbon compound, 18g of nano cellulose, 23g of sodium alginate and 12.8g of beta-glucan into 80g of water, carrying out ultrasonic treatment for 20min under the conditions of 60Hz and 1800W, adding 2.5g of sodium tripolyphosphate, heating to 100 ℃, cooling to room temperature after reaction for 3 hours, adding 40g of propolis, and stirring and mixing at the room temperature at the rotating speed of 700rpm for 2 hours to obtain a mixed system;
3) And 2) carrying out electrostatic spinning on the mixed system obtained in the step 2), applying 18KV high voltage in the electrostatic spinning process, enabling the distance between a nozzle of an injector and a receiving device to be 12cm, obtaining composite nano fibers, and then manufacturing the composite nano fibers into a sheet-shaped structure to obtain the impurity removing agent.
Comparative example 3
A preparation method of a compound plant extract comprises the following steps:
mixing 500g corn silk and 500g tartary buckwheat seeds, washing with water, spraying 5g of 1.0wt% beta-cyclodextrin aqueous solution on the surface, standing for 12h, freeze-drying at-20 ℃ for 2h, immediately crushing after freeze-drying, sieving with a 80-mesh sieve, adding water according to a feed-liquid ratio of 1:15g/mL, and carrying out ultrasonic extraction for 2h at room temperature under the conditions of 80Hz and 120W; extracting under high-voltage pulse electric field with electric field intensity of 30kV/cm, pulse time of 500 mus and pulse frequency of 300Hz for 20min, regulating pH to 6 with 36wt% acetic acid aqueous solution to obtain mixture, adding mixed bacterial liquid prepared by mixing Lactobacillus delbrueckii bacterial liquid and Basidiomycetes bacterial liquid with mass ratio of 1:2 into the mixture, fermenting at 30deg.C for 10d, adding impurity removing agent into the fermentation liquid after fermentation, adding impurity removing agent in an amount of 5 per mill of the mass of the fermentation liquid, stirring for 10min, filtering, steaming the filtrate under reduced pressure to remove solvent, pasteurizing at 70deg.C for 20min, and freeze drying at-20deg.C for 12h to obtain compound plant extract.
The preparation method of the lactobacillus shiitake fungus solution comprises the following steps:
lactobacillus shiitake is inoculated on a culture medium which is formed by mixing 3 weight percent of glucose, 2.5 weight percent of fish peptone, 1.5 weight percent of yeast extract, 0.3 weight percent of monopotassium phosphate, 0.2 weight percent of calcium carbonate and the balance of water, the inoculation amount is 2 weight percent, and the lactobacillus shiitake bacterial liquid is obtained by culturing for 36 hours at the temperature of 30 ℃.
The preparation method of the basidiomycete liquid comprises the following steps:
inoculating the Basidiomycetes in a culture medium prepared by uniformly mixing 8wt% of skimmed milk powder, 3wt% of inulin, 0.8wt% of sodium dihydrogen phosphate, 0.5wt% of magnesium sulfate and the balance of water, wherein the inoculum size is 1.5wt%, and culturing for 30 hours at 30 ℃ to obtain the Basidiomycetes liquid.
The preparation method of the impurity removing agent comprises the following steps:
1) Adding 18g of nanocellulose, 23g of sodium alginate and 12.8g of beta-glucan into 80g of water, carrying out ultrasonic treatment for 20min under the conditions of 60Hz and 1800W, adding 2.5g of sodium tripolyphosphate, heating to 100 ℃, reacting for 3h, cooling to room temperature, adding 40g of propolis, and stirring and mixing at the room temperature at the rotating speed of 700rpm for 2h to obtain a mixed system;
2) And (3) carrying out electrostatic spinning on the mixed system obtained in the step (1), applying 18KV high voltage in the electrostatic spinning process, enabling the distance between a nozzle of an injector and a receiving device to be 12cm, obtaining composite nano fibers, and then manufacturing the composite nano fibers into a sheet-shaped structure to obtain the impurity removing agent.
Comparative example 4
A preparation method of a compound plant extract comprises the following steps:
mixing 500g corn silk and 500g tartary buckwheat seeds, washing with water, spraying 5g of 1.0wt% beta-cyclodextrin aqueous solution on the surface, standing for 12h, freeze-drying at-20 ℃ for 2h, immediately crushing after freeze-drying, sieving with a 80-mesh sieve, adding water according to a feed-liquid ratio of 1:15g/mL, and carrying out ultrasonic extraction for 2h at room temperature under the conditions of 80Hz and 120W; extracting under high-voltage pulse electric field with electric field intensity of 30kV/cm, pulse time of 500 mus and pulse frequency of 300Hz for 20min, regulating pH to 6 with 36wt% acetic acid aqueous solution to obtain mixture, adding mixed bacterial liquid prepared by mixing Lactobacillus delbrueckii bacterial liquid and Basidiomycetes bacterial liquid with mass ratio of 1:2 into the mixture, fermenting at 30deg.C for 10d, adding impurity removing agent into the fermentation liquid after fermentation, adding impurity removing agent in an amount of 5 per mill of the mass of the fermentation liquid, stirring for 10min, filtering, steaming the filtrate under reduced pressure to remove solvent, pasteurizing at 70deg.C for 20min, and freeze drying at-20deg.C for 12h to obtain compound plant extract.
The preparation method of the lactobacillus shiitake fungus solution comprises the following steps:
lactobacillus shiitake is inoculated on a culture medium which is formed by mixing 3 weight percent of glucose, 2.5 weight percent of fish peptone, 1.5 weight percent of yeast extract, 0.3 weight percent of monopotassium phosphate, 0.2 weight percent of calcium carbonate and the balance of water, the inoculation amount is 2 weight percent, and the lactobacillus shiitake bacterial liquid is obtained by culturing for 36 hours at the temperature of 30 ℃.
The preparation method of the basidiomycete liquid comprises the following steps:
inoculating the Basidiomycetes in a culture medium prepared by uniformly mixing 8wt% of skimmed milk powder, 3wt% of inulin, 0.8wt% of sodium dihydrogen phosphate, 0.5wt% of magnesium sulfate and the balance of water, wherein the inoculum size is 1.5wt%, and culturing for 30 hours at 30 ℃ to obtain the Basidiomycetes liquid.
The preparation method of the impurity removing agent comprises the following steps:
1) Under argon atmosphere, mixing 100g of glucose with 100g of ammonium chloride, placing in a tube furnace, heating to 900 ℃ at a heating rate of 250 ℃/min, then reacting at 900 ℃ for 3 hours, cooling to room temperature to obtain a bubble-like substance, and then placing the bubble-like substance in air atmosphere and oxidizing at 400 ℃ for 8 hours to obtain a nano carbon compound;
2) Adding 12.5g of nano carbon compound, 18g of nano cellulose, 23g of sodium alginate and 12.8g of beta-glucan into 80g of water, carrying out ultrasonic treatment for 20min under the conditions of 60Hz and 1800W, adding 2.5g of sodium tripolyphosphate, heating to 100 ℃, reacting for 3h, and cooling to room temperature to obtain a mixed system;
3) And 2) carrying out electrostatic spinning on the mixed system obtained in the step 2), applying 18KV high voltage in the electrostatic spinning process, enabling the distance between a nozzle of an injector and a receiving device to be 12cm, obtaining composite nano fibers, and then manufacturing the composite nano fibers into a sheet-shaped structure to obtain the impurity removing agent.
Comparative example 5
A preparation method of a compound plant extract comprises the following steps:
mixing 500g corn silk and 500g tartary buckwheat seeds, washing with water, spraying 5g of 1.0wt% beta-cyclodextrin aqueous solution on the surface, standing for 12h, freeze-drying at-20 ℃ for 2h, immediately crushing after freeze-drying, sieving with a 80-mesh sieve, adding water according to a feed-liquid ratio of 1:15g/mL, and carrying out ultrasonic extraction for 2h at room temperature under the conditions of 80Hz and 120W; extracting for 20min under a high-voltage pulse electric field with the electric field intensity of 30kV/cm, the pulse time of 500 mus and the pulse frequency of 300Hz, regulating the pH value to 6 by using 36wt% acetic acid aqueous solution to obtain a mixture, adding mixed bacterial liquid which is prepared by mixing lactobacillus helveticus bacterial liquid and basidiomycete liquid with the mass ratio of 1:2, fermenting for 10d at the temperature of 30 ℃ with the addition of 3% of the mass of the mixed bacterial liquid, filtering after fermentation, evaporating the filtrate under reduced pressure to remove the solvent, pasteurizing for 20min at the temperature of 70 ℃, and finally freeze-drying for 12h at the temperature of minus 20 ℃ to obtain the composite plant extract.
The preparation method of the lactobacillus shiitake fungus solution comprises the following steps:
lactobacillus shiitake is inoculated on a culture medium which is formed by mixing 3 weight percent of glucose, 2.5 weight percent of fish peptone, 1.5 weight percent of yeast extract, 0.3 weight percent of monopotassium phosphate, 0.2 weight percent of calcium carbonate and the balance of water, the inoculation amount is 2 weight percent, and the lactobacillus shiitake bacterial liquid is obtained by culturing for 36 hours at the temperature of 30 ℃.
The preparation method of the basidiomycete liquid comprises the following steps:
inoculating the Basidiomycetes in a culture medium prepared by uniformly mixing 8wt% of skimmed milk powder, 3wt% of inulin, 0.8wt% of sodium dihydrogen phosphate, 0.5wt% of magnesium sulfate and the balance of water, wherein the inoculum size is 1.5wt%, and culturing for 30 hours at 30 ℃ to obtain the Basidiomycetes liquid.
Test example 1
50g of fresh rose is dried at 60 ℃ for 8 hours, then is respectively mixed with 30g of the compound plant extract prepared in the example 1 and the comparative examples 1-5 and 10g of Zhengshan black tea, ground into powder, 5g of the powder is taken, and the powder is brewed with 250mL of 100 ℃ water for 10 minutes to obtain rose tea, and the light transmittance is tested:
light transmittance test: taking 10mL of the rose tea in a cuvette by adopting a spectrophotometry, taking distilled water as a reference solution, and measuring the light transmittance of the rose tea at 720nm, wherein the light transmittance is expressed as T (%); the test results are shown in table 1:
table 1 light transmittance test results
| T(％) | |
| Example 1 | 93 |
| Comparative example 1 | 91.8 |
| Comparative example 2 | 90.2 |
| Comparative example 3 | 80.3 |
| Comparative example 4 | 85.1 |
| Comparative example 5 | 50.6 |
From the experimental results in table 1, it can be seen that the rose tea containing the compound plant extract prepared in example 1 has the best light transmittance, and the possible reason for this phenomenon is that the porous structure of the nano carbon compound can adsorb heavy metals and other bacteria in the plant extract, sodium alginate and beta-glucan can promote the propolis to form a compound film to be attached to the surface of materials formed by the nano carbon compound, nano cellulose and the like, active groups on the surface of the propolis can purify the compound plant extract system, and can react with bitter and astringent polyphenol peroxide in the compound plant extract, so that the peroxidized polyphenol and unstable pigment substances in the extract can be effectively removed, and the prepared rose tea has better glossiness.
Test example 2
And (3) blood pressure reducing effect test: the rose tea containing the compound plant extracts prepared in example 1 and comparative examples 1 to 5 of the present invention obtained in test example 1 was tested for blood pressure lowering effect, and the test subjects were SHR male rats, SPF grade, and body weight of 300-400 g/mouse, and the control group was selected from common male Wistar rats, SPF grade, and body weight of 300-400 g/mouse, and SHR rats and Wistar rats were supplied from Shanghai laboratory animal center of the national academy of sciences. Randomly taking 15 Wistar rats as normal groups; 105 rats suffering from hypertension SHR are randomly selected and divided into 6 groups of experimental groups and 1 group of model groups, 15 rats in each group are divided into a normal group, a model group and an experimental group, basic feed is normally given daily to the normal group, the model group and the experimental group and free drinking water is carried out, and the feeding environment is 25 ℃. The experimental groups were additionally fed daily with rose tea containing the compound plant extract of example 1, comparative examples 1-5 at a dose of 8mL/10g body weight, continuously fed weekly for 5 days, stopped for 2 days, treated for 4 weeks, and each group was tested weekly for blood pressure (systolic blood pressure). The blood pressure test of the rat is carried out by using a BP-2010A type animal noninvasive sphygmomanometer (supplied by Shenzhen Ruiword life technology Co., ltd.) and the result is calculated as an average value of a plurality of times, the blood pressure measurement result of the SHR rat is shown in Table 2, and the test result has statistical significance.
Table 2 blood pressure lowering effect test results table
As can be seen from the blood pressure lowering effect test results in Table 2, the rose tea prepared by the invention can effectively control blood pressure, and the blood pressure of SHR rats is gradually recovered and approaches normal value after the rose tea added with the compound plant extract prepared in example 1 is continuously fed for 4 weeks, probably because the Lactobacillus helveticus and the Basidiomycetes can promote the conversion of glutamic acid in corn silk and tartary buckwheat into gamma-aminobutyric acid, and the gamma-aminobutyric acid is an important inhibitory neurotransmitter in the central nervous system, has the effects of anticonvulsant, promoting cerebral metabolism, preventing cerebral arteriosclerosis and obviously inhibiting blood pressure rise, improves the blood pressure lowering effect of the compound plant extract, and removes a large amount of impurities and pigments by adding the impurity removing agent, so that the content of gamma-aminobutyric acid is higher, and further improves the blood pressure lowering effect.
Claims (10)
1. A method for preparing a composite plant extract, which is characterized by comprising the following steps: mixing corn silk and tartary buckwheat seeds, washing with water, spraying a beta-cyclodextrin aqueous solution with the concentration of 0.5-1.5wt% on the surface, standing for 10-12h, freeze-drying at-30-20 ℃ for 1-2h, immediately crushing after freeze-drying, sieving with a 80-100 mesh sieve, adding water according to the feed liquid ratio of 1:10-15g/mL, and performing ultrasonic extraction for 1-2h at room temperature, 60-100Hz and 120-150W; extracting under high-voltage pulse electric field for 10-30min, regulating pH to 5-7 with 30-36wt% acetic acid aqueous solution to obtain mixture, adding mixed bacterial liquid into the mixture, fermenting at 25-30deg.C for 8-10d, adding impurity removing agent into the fermentation broth after fermentation, stirring for 10-20min, filtering, steaming filtrate under reduced pressure to remove solvent, pasteurizing at 60-80deg.C for 20-30min, and freeze drying at-30 to-20deg.C for 6-12 hr to obtain composite plant extract.
2. The method for preparing a composite plant extract according to claim 1, wherein: the dosage ratio of the corn silk to the tartary buckwheat seeds to the beta-cyclodextrin aqueous solution is 500-1000:500-1000:5-10.
3. The method for preparing a composite plant extract according to claim 1, wherein: the high-voltage pulse electric field extraction condition is that the electric field intensity is 20-40kV/cm, the pulse time is 400-500 mu s, and the pulse frequency is 200-300Hz.
4. The method for preparing a composite plant extract according to claim 1, wherein: the mass ratio of the mixed bacterial liquid is 1-2: mixing the 2-3 Xihe lactobacillus strain solution with the Basidiomycetes solution.
5. The method for preparing a composite plant extract according to claim 4, wherein the method for preparing the lactobacillus johnsonii bacterial liquid comprises the following steps:
inoculating Lactobacillus helveticus to a culture medium prepared by mixing 2-5wt% glucose, 2-3wt% fish peptone, 1-2wt% yeast extract, 0.1-0.5wt% potassium dihydrogen phosphate, 0.1-0.5wt% calcium carbonate and the balance water, wherein the inoculation amount is 1-3wt%, and culturing at 25-35deg.C for 24-36 hr to obtain Lactobacillus helveticus bacterial liquid.
6. The method for preparing a composite plant extract according to claim 4, wherein the method for preparing the basidiomycete liquid comprises the following steps:
inoculating Basidiomycetes in a culture medium prepared by uniformly mixing 6-10wt% of skimmed milk powder, 2-5wt% of inulin, 0.5-1.2wt% of sodium dihydrogen phosphate, 0.1-1.0wt% of magnesium sulfate and the balance of water, wherein the inoculation amount is 1-2wt%, and culturing at 25-35deg.C for 24-36 hr to obtain Basidiomycetes liquid.
7. The method for preparing a composite plant extract according to claim 1, wherein: the addition amount of the impurity removing agent is 1-3 per mill of the mass of the fermentation liquor.
8. The method for preparing a composite plant extract according to claim 7, wherein the method for preparing the impurity removing agent comprises the steps of:
1) Mixing 100-120g of glucose and 100-120g of ammonium chloride in an argon atmosphere, placing in a tube furnace, heating to 800-900 ℃ at a heating rate of 200-250 ℃/min, reacting at 800-900 ℃ for 3-5h, cooling to room temperature to obtain a bubble, and oxidizing the bubble in an air atmosphere at 300-400 ℃ for 6-8h to obtain a nano carbon compound;
2) Adding 10-15g of nano carbon compound, 15-20g of nano cellulose, 20-25g of sodium alginate and 10-15g of beta-glucan into 50-80g of water, carrying out ultrasonic treatment for 20-30min under the conditions of 50-80Hz and 1500-2000W, adding 1-3g of sodium tripolyphosphate, heating to 80-100 ℃, reacting for 2-4h, cooling to room temperature, adding 30-50g of propolis, stirring and mixing at the room temperature at the rotation speed of 600-800rpm for 1-2h, and obtaining a mixed system;
3) And (3) carrying out electrostatic spinning on the mixed system obtained in the step (2), applying 15-20KV high voltage in the electrostatic spinning process, enabling the distance between a nozzle of an injector and a receiving device to be 10-15cm, obtaining composite nano fibers, and then manufacturing the composite nano fibers into a sheet-shaped structure to obtain the impurity removing agent.
9. A composite plant extract prepared by the method of any one of claims 1-8.
10. The use of the compound plant extract according to claim 9 in heat clearing rose tea, characterized by the specific application as follows: drying fresh flos Rosae Rugosae and plant with heat clearing function, mixing with the compound plant extract of claim 9 and black tea, grinding into powder, and packaging.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310186203.3A CN116491567A (en) | 2023-03-01 | 2023-03-01 | Preparation method of composite plant extract and application of composite plant extract in heat-clearing rose tea |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310186203.3A CN116491567A (en) | 2023-03-01 | 2023-03-01 | Preparation method of composite plant extract and application of composite plant extract in heat-clearing rose tea |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN116491567A true CN116491567A (en) | 2023-07-28 |
Family
ID=87319121
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310186203.3A Pending CN116491567A (en) | 2023-03-01 | 2023-03-01 | Preparation method of composite plant extract and application of composite plant extract in heat-clearing rose tea |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN116491567A (en) |
-
2023
- 2023-03-01 CN CN202310186203.3A patent/CN116491567A/en active Pending
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| KR101738594B1 (en) | Prepatation method of black platycodon syrup and black platycodon syrup | |
| KR20070114334A (en) | The manufacturing process of extrusion puffed and fermented red ginseng and mountain ginseng extracts | |
| CN110122803B (en) | Noni tablet and preparation method thereof | |
| KR101799424B1 (en) | Astragalus membranaceus Fermentation product and its manufacturing method | |
| CN116064685B (en) | Preparation process and application of eurotium cristatum fermented edible traditional Chinese medicine | |
| US20210123002A1 (en) | Appetizing and summer heat relieving tangxin raw cider and high-efficiency deep processing technology thereof | |
| CN101084935A (en) | Pine needle extract and its preparing process and application | |
| CN105754809A (en) | Organic selenium-rich original wine with Tibetan cordyceps and method for industrially producing organic selenium-rich original wine | |
| CN105559068B (en) | It is a kind of to utilize composition for eating the acquisition of medicine fungi fermentation radix tetrastigme and preparation method thereof | |
| CN112956627A (en) | Compound solid drink of chaga for women | |
| CN111227080A (en) | Agaricus blazei murill fermented mulberry leaf tea and production method thereof | |
| CN110041727A (en) | A kind of method of ultrasound assisted extraction beet red pigment | |
| KR101404309B1 (en) | The method of preparing clear and cool drink using the traditional oriental medicine of plant including polypara codata bueck | |
| CN116491567A (en) | Preparation method of composite plant extract and application of composite plant extract in heat-clearing rose tea | |
| CN106497722B (en) | Walnut flower pomegranate fruit wine and preparation method thereof | |
| KR102147880B1 (en) | Manufacturing method of functional ginseng toenjang fused with zinc and selenium | |
| KR102147881B1 (en) | Manufacturing method of functional ginseng soy sauce fused with zinc and selenium | |
| CN108308485A (en) | A kind of linseed plant beverage and preparation method thereof | |
| CN109937795B (en) | Method for cultivating cordyceps sinensis mycelia by taking jasmine as matrix | |
| CN111084739B (en) | Composition for enhancing skin elasticity or improving skin wrinkles comprising enzyme-treated extract of roots of leguminous plants | |
| EP2132994B1 (en) | Fermentation of solutions containing pomegranate juice with saccharomyces boulardii and lactobacilli, resulting fermented products and application thereof | |
| CN103798520B (en) | A kind of feed additive of substitute antibiotics and preparation method thereof | |
| CN112840947A (en) | Method for cultivating cordyceps sinensis by taking hawthorn as main matrix | |
| CN103800422B (en) | Compound electuary preparation of eucommia health care and products thereof | |
| CN111887367A (en) | Loquat flower beverage and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |