CN116478811B - Temperature control plate and EP tube assembly for PCR amplification, manufacturing method and PCR amplification instrument - Google Patents
Temperature control plate and EP tube assembly for PCR amplification, manufacturing method and PCR amplification instrument Download PDFInfo
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- CN116478811B CN116478811B CN202310743313.5A CN202310743313A CN116478811B CN 116478811 B CN116478811 B CN 116478811B CN 202310743313 A CN202310743313 A CN 202310743313A CN 116478811 B CN116478811 B CN 116478811B
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- pcr amplification
- heat conduction
- temperature control
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- 238000012408 PCR amplification Methods 0.000 title claims abstract description 37
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 16
- 238000000034 method Methods 0.000 claims abstract description 8
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 12
- 239000000741 silica gel Substances 0.000 claims description 12
- 229910002027 silica gel Inorganic materials 0.000 claims description 12
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 claims description 7
- 229910052782 aluminium Inorganic materials 0.000 claims description 7
- 239000007788 liquid Substances 0.000 claims description 7
- 230000003321 amplification Effects 0.000 abstract description 15
- 238000003199 nucleic acid amplification method Methods 0.000 abstract description 15
- 238000001514 detection method Methods 0.000 abstract description 8
- 230000002411 adverse Effects 0.000 abstract description 3
- 239000012295 chemical reaction liquid Substances 0.000 abstract description 3
- 230000005489 elastic deformation Effects 0.000 abstract description 2
- 230000000630 rising effect Effects 0.000 abstract description 2
- 230000000694 effects Effects 0.000 description 8
- 238000010438 heat treatment Methods 0.000 description 7
- 239000000463 material Substances 0.000 description 5
- 238000012986 modification Methods 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 238000010586 diagram Methods 0.000 description 2
- 241000238097 Callinectes sapidus Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 239000000945 filler Substances 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 230000017525 heat dissipation Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 229920001296 polysiloxane Polymers 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 238000007711 solidification Methods 0.000 description 1
- 230000008023 solidification Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
- B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
- B01L3/50851—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates specially adapted for heating or cooling samples
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/12—Specific details about materials
- B01L2300/123—Flexible; Elastomeric
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
- B01L2300/1822—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using Peltier elements
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Hematology (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The invention provides a temperature control plate and EP tube assembly for PCR amplification, a manufacturing method and a PCR amplification instrument, wherein the temperature control plate and the EP tube assembly for PCR amplification comprise a heat conduction plate body, a plurality of rows of cavity holes are formed in the heat conduction plate body at intervals, each cavity hole is correspondingly provided with each reaction tube body in a multi-row EP tube, and the temperature control plate and EP tube assembly further comprises an elastic heat conduction layer, and the elastic heat conduction layer is clamped between the inner wall of the cavity hole and the outer wall of the reaction tube body. According to the invention, the elastic heat conduction layer is arranged between the inner wall of the cavity hole and the outer wall of the reaction tube body, and the elastic heat conduction layer can thoroughly contact with the cavity hole and the reaction tube body due to elastic deformation, so that the reaction liquid in the reaction tube body is uniformly and efficiently heated, the adverse phenomena of poor repeatability between holes, unstable or inaccurate quantitative detection result and the like in the prior art are avoided, the temperature rising and falling speed is faster in the amplification process, and the amplification stability and the amplification efficiency are greatly improved.
Description
Technical Field
The invention belongs to the technical field of PCR amplification, and particularly relates to a temperature control plate and EP tube assembly for PCR amplification, a manufacturing method and a PCR amplification instrument.
Background
PCR amplification is one of the most common biological molecular techniques for amplifying DNA fragments, and is an extremely common conventional technical means for in vitro replication and greatly amplifying DNA. The PCR amplification apparatus of various types developed based on the technology is a high-precision temperature control device.
PCR amplification instruments generally enhance two parameters when improving their device performance: first, raising the heating rate, for example, the heating rate of company products can reach 8 ℃/s; secondly, controlling heating precision, adopting imported advanced custom-made heating Peltier and the like, and accurately controlling the set temperature to reduce the temperature overshoot effect. However, the lifting of the instrument eventually encounters bottlenecks, namely, the aluminum cavity and the EP pipe made of PP material are in hard contact to cause uneven heat transfer and heat dissipation, a large number of gaps exist between the aluminum cavity and the EP pipe, and the like, so that samples in the EP pipe are heated unevenly when the samples are heated, the amplification effects of different positions of the multi-row EP pipe are different, and the final experimental effect can often appear poor phenomena of poor repeatability among holes, unstable or inaccurate quantitative detection results, and the like.
Disclosure of Invention
Therefore, the technical problem to be solved by the invention is to provide a temperature control plate and an EP tube assembly for PCR amplification, a manufacturing method and a PCR amplification instrument, so as to overcome the defects that in the prior art, a large number of gaps exist between a cavity of the temperature control plate for PCR amplification and an EP tube in hard contact, so that samples in the EP tube are heated unevenly, amplification effects of different holes of the multi-row EP tube are inconsistent, hole repeatability is poor, and quantitative detection results are unstable or inaccurate.
In order to solve the problems, the invention provides a temperature control plate and EP tube assembly for PCR amplification, which comprises a heat conduction plate body, wherein a plurality of rows of cavity holes are formed in the heat conduction plate body at intervals, each cavity hole is correspondingly provided with each reaction tube body in the multi-row EP tube, and the temperature control plate and EP tube assembly further comprises an elastic heat conduction layer, wherein the elastic heat conduction layer is clamped between the inner wall of each cavity hole and the outer wall of each reaction tube body.
In some embodiments, the elastic heat conducting layer is coated on the inner wall of the cavity hole.
In some embodiments, the elastic heat conductive layer is formed of silicone.
In some embodiments, the elastic heat conductive layer has a minimum thickness of not less than 0.05mm and a maximum thickness of not more than 0.2mm.
In some embodiments, when the elastic heat conductive layer is made of silica gel, a thickness of the elastic heat conductive layer corresponding to a side wall of the reaction tube body is between 0.05mm and 0.1mm, and a thickness of the elastic heat conductive layer corresponding to a bottom wall of the reaction tube body is between 0.1 and 0.2mm.
In some embodiments, the material of the heat-conducting plate body is aluminum.
In some embodiments, the elastic heat conducting layer is applied on the inner wall of the cavity hole in a sticking or pouring mode.
The invention also provides a PCR amplification instrument, which comprises the temperature control plate for PCR amplification.
The invention also provides a manufacturing method of the temperature control plate for PCR amplification, which comprises the following steps:
manufacturing and forming a heat-conducting plate body;
filling preset amount of silica gel liquid into each cavity hole of the heat-conducting plate body;
and (3) seating each reaction tube body of the multi-row EP tube in each cavity hole, and removing the multi-row EP tube from the heat-conducting plate body after the silica gel liquid is solidified.
In some embodiments, the outer wall of each of the reaction tubes of the multi-row EP tube is subjected to an anti-sticking treatment prior to seating the reaction tube in each of the cavity holes.
According to the temperature control plate and the EP tube assembly for PCR amplification, the elastic heat conduction layer is arranged between the inner wall of the cavity hole and the outer wall of the reaction tube body, the elastic heat conduction layer can thoroughly contact with the cavity hole and the reaction tube body due to elastic deformation, gaps caused by hard contact between the reaction tube body and the cavity hole in the prior art are thoroughly eliminated, heat conducted in the heat conduction plate body can be conducted to the reaction tube body more uniformly and efficiently through the elastic heat conduction layer, uniform and efficient heating of reaction liquid in the reaction tube body is further realized, the adverse phenomena of poor repeatability between holes, unstable or inaccurate quantitative detection result and the like in the prior art are avoided, the temperature rising and falling rate in the amplification process is higher, and the amplification stability and the amplification efficiency are greatly improved.
Drawings
Fig. 1 is a schematic structural diagram of a temperature control plate for PCR amplification and an EP tube assembly according to an embodiment of the present invention, in which the thickness of an elastic heat conducting layer is exaggerated for convenience of expression.
FIG. 2 is a schematic diagram of a temperature-controlled plate for PCR amplification according to an embodiment of the present invention.
FIG. 3 shows the results of PCR amplification without using the technical scheme of the present invention.
FIG. 4 shows the results of PCR amplification performed using the technical scheme of the present invention.
The reference numerals are: 1. a heat conductive plate body; 11. a cavity hole; 12. an elastic heat conductive layer; 101. a reaction tube body.
Description of the embodiments
Referring to fig. 1 to 4 in combination, according to an embodiment of the present invention, a temperature control plate and EP tube assembly for PCR amplification is provided, which includes a heat conduction plate body 1 in heat transfer connection with a peltier isothermal control assembly, a plurality of rows of spaced cavity holes 11 are formed in the heat conduction plate body 1, the positions and sizes of the cavity holes 11 are matched with the types of the multi-row EP tubes, each cavity hole 11 accommodates each reaction tube 101 in the multi-row EP tube in a one-to-one correspondence manner, and an elastic heat conduction layer 12 is further included, and the elastic heat conduction layer 12 is clamped between the inner wall of the cavity hole 11 and the outer wall of the reaction tube 101.
In this technical scheme, through setting up elasticity heat conduction layer 12 between the hole inner wall of die cavity hole 11 and the outer wall of reaction tube body 101, because elasticity deformation of elasticity heat conduction layer 12 makes its contact with die cavity hole 11 and reaction tube body 101 thoroughly, thoroughly eliminate the gap that the hard contact between reaction tube body 101 and the die cavity hole 11 brought among the prior art, make the heat of conduction in the heat conduction plate body 1 can be more even high-efficient through elasticity heat conduction layer 12 conduction to reaction tube body 101, and then realize the even high-efficient heating of the reaction liquid in the reaction tube body 101, the bad phenomenon such as poor, quantitative detection result unstable or inaccurate appear among the hole that appear among the prior art has been stopped, the increase temperature rate is faster in the amplification process, greatly improved the stability of amplification and the efficiency of amplification.
The elastic heat conducting layer 12 is preferably realized by adopting a heat conducting and wear-resisting soft filler, and the wear resistance can prolong the service life of the temperature control plate.
The aforementioned elastic heat conductive layer 12 may be applied to the outer wall of each reaction tube 101, but this way is cumbersome to manufacture and the manufacturing cost of the components is high because the multi-row EP tube is a disposable consumable. It is therefore more preferable to apply the elastic heat conductive layer 12 to the inner wall of the cavity hole 11 so that the arrangement thereof can be repeatedly used, reducing the manufacturing cost.
In one embodiment, the attachable film may be attached to the inner wall of the cavity 11, but in general, the inner wall of the cavity 11 is a tapered hole, and the attaching process is difficult and cannot ensure complete covering of the pipe wall, so that it is preferable to form the attachable film by filling the corresponding material.
As a preferred embodiment, the elastic heat conducting layer 12 is made of silica gel, which has high wear resistance, deformation force and heat conducting property, and can be formed in a filling manner, so that the production is more convenient.
In some embodiments, the elastic thermally conductive layer 12 has a minimum thickness of no less than 0.05mm and a maximum thickness of no more than 0.2mm. When the minimum thickness of the elastic heat conducting layer 12 is lower than 0.05mm, the gap between the outer wall of the tube and the inner wall of the hole cannot be effectively filled by the excessive thickness, so that the self-adaptive EP tube appearance cannot achieve the complete wrapping effect, and the excessive thickness can form excessive occupation of the hole space of the cavity hole 11, so that the expandable volume of the EP tube is influenced. Further, when the elastic heat-conducting layer 12 is made of silica gel, the thickness of the elastic heat-conducting layer 12 corresponding to the side wall of the reaction tube body 101 is between 0.05mm and 0.1mm, and the thickness of the elastic heat-conducting layer 12 corresponding to the bottom wall of the reaction tube body 101 is between 0.1 and 0.2mm, so that the adverse effect on the expansion volume formation can be reduced to the greatest extent by the elastic heat-conducting layer 12, and meanwhile, the thickness of the elastic heat-conducting layer 12 corresponding to the bottom wall of the reaction tube body 101 is not lower than the thickness of the elastic heat-conducting layer 12 corresponding to the side wall of the reaction tube body 101, so that the hole bottom of the cavity hole 11 and the bottom wall of the reaction tube body 101 form a more complete and thorough package through the elastic heat-conducting layer 12, and the temperature regulation efficiency is further improved. It can be understood that the thickness of the elastic heat conductive layer 12 corresponding to the side wall and the elastic heat conductive layer 12 corresponding to the bottom wall should be continuously and smoothly graded, and no step should be formed.
In some embodiments, the material of the heat-conducting plate body 1 is aluminum, and aluminum is used as the manufacturing material of the heat-conducting plate body 1, which has the advantages of small mass and high heat conductivity.
According to an embodiment of the invention, there is also provided a PCR amplification apparatus including the above-mentioned temperature control plate for PCR amplification and an EP tube assembly.
According to an embodiment of the present invention, there is also provided a method for manufacturing a temperature control plate for PCR amplification and an EP tube assembly, including the steps of:
manufacturing and forming the heat-conducting plate body 1; the preset amount of silica gel liquid is filled in each cavity hole 11 of the heat conducting plate body 1; the reaction tube bodies 101 of the multi-row EP tubes are seated in the cavity holes 11, and after the silica gel liquid is solidified, the multi-row EP tubes are moved away from the heat conducting plate body 1, and the heat conducting plate body 1 at this time becomes the heat conducting plate body 1 with the elastic heat conducting layer 12.
After the solidification, the silica gel liquid in the invention is adhered to the aluminum heat conducting plate body 1 and is not easy to adhere to a plastic product (the EP pipe is a plastic product), so after the multi-row EP pipe is moved away from the heat conducting plate body 1, the elastic heat conducting layer 12 is wrapped on the inner wall of the cavity hole 11 of the rest heat conducting plate part, and the heat conducting plate is stable, reliable and can be repeatedly used.
In some embodiments, before each reaction tube body 101 of the multi-row EP tube is seated in each cavity hole 11, an anti-adhesion treatment is performed on the outer wall of the reaction tube body 101, for example, the anti-adhesion treatment may be to coat a corresponding anti-adhesion agent on the outer wall of the tube or form a corresponding chemical anti-adhesion layer, etc., and adhesion between the silica gel and the EP tube can be further prevented by the anti-adhesion treatment, so that the complete smoothness of the outer surface of the elastic heat conducting layer 12 is facilitated.
Referring to fig. 3 and fig. 4 in combination, as shown in fig. 3, the amplification effect of a common PCR apparatus using a large-volume system in the prior art (i.e., the technical scheme of the present invention is not adopted) is expressed, and a large number of "tailing" (the area out of the oval inner circle in fig. 3) bad phenomena are shown in the amplification result, which are the phenomena that a part of targets do not reach the set target cycle times due to uneven heating during amplification, resulting in low fluorescence signal values, wherein the abscissa represents fluorescence signal values, the blue point represents a positive detection result of a target, the green point represents a positive detection result of a target two, the orange point represents a double positive result, the bad phenomena frequently occur in the large-volume system and are difficult to radically cure, and even only a positive result with low signal values can be "shielded" by a software algorithm in part of detection flow. The experimental results of PCR amplification performed by the technical scheme of the invention are shown in FIG. 4, and the experimental results show that the improvement is very obvious, the problems of poor amplification and low efficiency are fundamentally solved, the invention has low cost, obvious improvement effect and excellent stability, and the invention is very beneficial to popularization in industry.
It will be readily appreciated by those skilled in the art that the above advantageous ways can be freely combined and superimposed without conflict. The foregoing description of the preferred embodiments of the invention is not intended to be limiting, but rather is intended to cover all modifications, equivalents, and alternatives falling within the spirit and principles of the invention. The foregoing is merely a preferred embodiment of the present invention, and it should be noted that it will be apparent to those skilled in the art that modifications and variations can be made without departing from the technical principles of the present invention, and these modifications and variations should also be regarded as the scope of the invention.
Claims (7)
1. The temperature control plate and EP tube assembly for PCR amplification is characterized by comprising a heat conduction plate body (1), wherein a plurality of rows of cavity holes (11) are formed in the heat conduction plate body (1), each cavity hole (11) is correspondingly provided with each reaction tube body (101) in the multi-row EP tube, and the temperature control plate and EP tube assembly further comprises an elastic heat conduction layer (12), and the elastic heat conduction layer (12) is clamped between the inner wall of each cavity hole (11) and the outer wall of each reaction tube body (101); the minimum thickness of the elastic heat conduction layer (12) is not less than 0.05mm, and the maximum thickness is not more than 0.2mm; the elastic heat conduction layer (12) is made of silica gel, the thickness of the elastic heat conduction layer (12) corresponding to the side wall of the reaction tube body (101) is between 0.05mm and 0.1mm, and the thickness of the elastic heat conduction layer (12) corresponding to the bottom wall of the reaction tube body (101) is between 0.1 and 0.2mm.
2. The PCR amplification temperature control plate and EP tube assembly according to claim 1, wherein the elastic heat conductive layer (12) is applied on the hole inner wall of the cavity hole (11).
3. The PCR amplification temperature control plate and EP tube assembly according to claim 1, wherein the heat conducting plate body (1) is made of aluminum.
4. The PCR amplification temperature control plate and EP tube assembly according to claim 1, wherein the elastic heat conducting layer (12) is applied to the inner wall of the cavity hole (11) by means of adhesion or pouring.
5. A PCR amplification apparatus comprising the temperature control plate for PCR amplification according to any one of claims 1 to 4 and an EP tube assembly.
6. The method for manufacturing a temperature control plate for PCR amplification and an EP tube assembly according to claim 2, comprising the steps of:
manufacturing and forming a heat-conducting plate body (1);
filling a preset amount of silica gel liquid into each cavity hole (11) of the heat conducting plate body (1);
and (3) seating each reaction tube body (101) of the multi-row EP tube in each cavity hole (11), and moving the multi-row EP tube away from the heat conducting plate body (1) after the silica gel liquid is solidified.
7. The method according to claim 6, wherein the outer wall of each of the reaction tubes (101) of the multi-row EP tube is subjected to an anti-sticking treatment before the reaction tubes (101) are seated in the respective cavity holes (11).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310743313.5A CN116478811B (en) | 2023-06-21 | 2023-06-21 | Temperature control plate and EP tube assembly for PCR amplification, manufacturing method and PCR amplification instrument |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310743313.5A CN116478811B (en) | 2023-06-21 | 2023-06-21 | Temperature control plate and EP tube assembly for PCR amplification, manufacturing method and PCR amplification instrument |
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| Publication Number | Publication Date |
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| CN116478811A CN116478811A (en) | 2023-07-25 |
| CN116478811B true CN116478811B (en) | 2023-09-22 |
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| CN202310743313.5A Active CN116478811B (en) | 2023-06-21 | 2023-06-21 | Temperature control plate and EP tube assembly for PCR amplification, manufacturing method and PCR amplification instrument |
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| CN116814397B (en) * | 2023-08-25 | 2023-11-28 | 新羿制造科技（北京）有限公司 | PCR reaction tube and multi-row PCR tube |
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| CN211099126U (en) * | 2019-10-24 | 2020-07-28 | 中信湘雅生殖与遗传专科医院有限公司 | Test tube heating device |
| CN213921901U (en) * | 2020-11-11 | 2021-08-10 | 青岛宝创生物科技有限公司 | Fluorescent quantitative PCR kit |
| CN217809437U (en) * | 2022-06-07 | 2022-11-15 | 江苏格诺生物科技有限公司 | Nucleic acid constant temperature amplification detector |
| CN218432363U (en) * | 2022-07-29 | 2023-02-03 | 福州艾迪康医学检验实验室有限公司 | Sample storage box for APL (apoptosis inducing ligand) related fusion gene research |
| CN115851428A (en) * | 2023-02-27 | 2023-03-28 | 北京万泰生物药业股份有限公司 | Fluorescent quantitative PCR instrument |
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- 2023-06-21 CN CN202310743313.5A patent/CN116478811B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN211099126U (en) * | 2019-10-24 | 2020-07-28 | 中信湘雅生殖与遗传专科医院有限公司 | Test tube heating device |
| CN213921901U (en) * | 2020-11-11 | 2021-08-10 | 青岛宝创生物科技有限公司 | Fluorescent quantitative PCR kit |
| CN217809437U (en) * | 2022-06-07 | 2022-11-15 | 江苏格诺生物科技有限公司 | Nucleic acid constant temperature amplification detector |
| CN218432363U (en) * | 2022-07-29 | 2023-02-03 | 福州艾迪康医学检验实验室有限公司 | Sample storage box for APL (apoptosis inducing ligand) related fusion gene research |
| CN115851428A (en) * | 2023-02-27 | 2023-03-28 | 北京万泰生物药业股份有限公司 | Fluorescent quantitative PCR instrument |
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