CN116473082A - Auxiliary agent for accelerating elongation of pollen tube and formula thereof - Google Patents
Auxiliary agent for accelerating elongation of pollen tube and formula thereof Download PDFInfo
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- CN116473082A CN116473082A CN202310738988.0A CN202310738988A CN116473082A CN 116473082 A CN116473082 A CN 116473082A CN 202310738988 A CN202310738988 A CN 202310738988A CN 116473082 A CN116473082 A CN 116473082A
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- pollen
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- immunity protein
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- 239000012752 auxiliary agent Substances 0.000 title abstract description 12
- 101710116034 Immunity protein Proteins 0.000 claims abstract description 44
- 239000007788 liquid Substances 0.000 claims abstract description 22
- 230000001737 promoting effect Effects 0.000 claims abstract description 5
- 241000196324 Embryophyta Species 0.000 claims description 69
- 230000010152 pollination Effects 0.000 claims description 24
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 9
- 235000014443 Pyrus communis Nutrition 0.000 claims description 9
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 claims description 9
- 239000004327 boric acid Substances 0.000 claims description 9
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 9
- 239000001110 calcium chloride Substances 0.000 claims description 9
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 8
- 229930006000 Sucrose Natural products 0.000 claims description 8
- 108090000623 proteins and genes Proteins 0.000 claims description 7
- 102000004169 proteins and genes Human genes 0.000 claims description 7
- 239000000203 mixture Substances 0.000 claims description 6
- 244000018633 Prunus armeniaca Species 0.000 claims description 5
- 235000009827 Prunus armeniaca Nutrition 0.000 claims description 5
- 239000000126 substance Substances 0.000 claims description 5
- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 claims description 3
- 241000223218 Fusarium Species 0.000 claims description 3
- 102000012214 Immunoproteins Human genes 0.000 claims description 3
- 108010036650 Immunoproteins Proteins 0.000 claims description 3
- 241000223600 Alternaria Species 0.000 claims 1
- 241001465180 Botrytis Species 0.000 claims 1
- 235000009319 Terminalia catappa Nutrition 0.000 claims 1
- 244000277583 Terminalia catappa Species 0.000 claims 1
- 230000004720 fertilization Effects 0.000 abstract description 5
- 239000012460 protein solution Substances 0.000 abstract description 4
- 230000012010 growth Effects 0.000 abstract description 3
- 210000001672 ovary Anatomy 0.000 abstract description 3
- 230000035784 germination Effects 0.000 description 11
- 230000000694 effects Effects 0.000 description 9
- 235000013399 edible fruits Nutrition 0.000 description 8
- 238000002360 preparation method Methods 0.000 description 6
- 239000000243 solution Substances 0.000 description 5
- 208000035240 Disease Resistance Diseases 0.000 description 3
- 241000223602 Alternaria alternata Species 0.000 description 2
- 241000123650 Botrytis cinerea Species 0.000 description 2
- 101710172711 Structural protein Proteins 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 235000013339 cereals Nutrition 0.000 description 2
- 229930002875 chlorophyll Natural products 0.000 description 2
- 235000019804 chlorophyll Nutrition 0.000 description 2
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 2
- 230000019827 double fertilization forming a zygote and endosperm Effects 0.000 description 2
- 230000002015 leaf growth Effects 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 239000011259 mixed solution Substances 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 230000005082 stem growth Effects 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 244000144725 Amygdalus communis Species 0.000 description 1
- 235000011437 Amygdalus communis Nutrition 0.000 description 1
- 241000218922 Magnoliophyta Species 0.000 description 1
- 244000061176 Nicotiana tabacum Species 0.000 description 1
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 244000269722 Thea sinensis Species 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 235000020224 almond Nutrition 0.000 description 1
- 230000010154 cross-pollination Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 235000021552 granulated sugar Nutrition 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 231100000956 nontoxicity Toxicity 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 230000007198 pollen germination Effects 0.000 description 1
- 239000008213 purified water Substances 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 230000002786 root growth Effects 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 235000013616 tea Nutrition 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 230000035899 viability Effects 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/50—Isolated enzymes; Isolated proteins
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/002—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing a foodstuff as carrier or diluent, i.e. baits
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/06—Aluminium; Calcium; Magnesium; Compounds thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/14—Boron; Compounds thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P21/00—Plant growth regulators
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Plant Pathology (AREA)
- Environmental Sciences (AREA)
- Wood Science & Technology (AREA)
- Pest Control & Pesticides (AREA)
- Dentistry (AREA)
- Agronomy & Crop Science (AREA)
- Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- Biotechnology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Microbiology (AREA)
- Virology (AREA)
- Food Science & Technology (AREA)
- Toxicology (AREA)
- Botany (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Peptides Or Proteins (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to the technical field of agriculture, and particularly provides an auxiliary agent for accelerating the elongation of pollen tubes and a formula thereof. In the application provided by the invention, the plant immunity protein is used as an auxiliary agent for pollen tube elongation. The content of the plant immunity protein solution in the liquid pollen is between 0.02 and 3.3 g/L. The liquid pollen obtained by the invention is sprayed on the column heads of the flowers of the target plants, so that the extension of the pollen tube is accelerated, the time for the pollen tube to reach the ovary is shortened, and the fertilization of the flowers is accelerated. The plant immunity protein-containing auxiliary agent provided by the invention can well promote the growth of pollen tubes, and is simple in source, safe and environment-friendly. The invention has low cost and can generate larger economic benefit. The invention applies the plant immunity protein to the elongation of the pollen tube for the first time, widens the application field of promoting the elongation of the pollen tube, and is suitable for popularization and application.
Description
Technical Field
The invention relates to the technical field of agriculture, in particular to an auxiliary agent for accelerating the elongation of pollen tubes and a formula thereof.
Background
The plant immunity protein is a novel structural protein separated and extracted from microorganism by biotechnology. It can activate the molecular immunity and metabolism regulation system in plant, raise disease resistance of plant, promote plant root, stem and leaf growth and chlorophyll content and raise crop yield. In recent years, a great deal of reports prove that the plant immunity protein can obviously promote plant growth, improve crop quality, improve disease resistance of crops and improve crop yield when being used on rice, fruits, vegetables, tobacco and tea.
Pollen tube (polen tube) is a tubular structure formed by projecting the inner wall of germinated pollen grains, and the inner wall of pollen grains is extended outwards through germination holes (or grooves) on the outer wall of pollen and is extended out from the germination holes. Pollen is placed on the stigma and is germinated firstly, after germination, a pollen tube is formed by extending the style towards the direction of an ovary body, the growth rate of the pollen tube determines the time required for completing double fertilization, and the fertilization time is of great significance for flowering plants in early spring, for example, the double fertilization process can be completed as soon as possible, the influence of severe environment on pollination and fertilization of the pollen tube can be avoided, and the fruit setting rate is improved. Therefore, the elongation speed of the pollen tube has important significance on whether fruit can be set as soon as possible, and is also beneficial to avoiding the influence of adverse environmental conditions in the later stage on fruit setting.
Previous studies have shown that a concentration of a plant immunity protein can increase the germination rate of pollen, in a sense that only the viability and germination rate of pollen are improved, but it is not reported whether a plant immunity protein accelerates the elongation of pollen tubes, i.e. the effect of a plant immunity protein in pollen tube elongation is not known.
Disclosure of Invention
The invention provides an auxiliary agent for accelerating the elongation of a pollen tube and a formula thereof, which are used for solving the defect that the auxiliary agent special for the elongation of the pollen tube is not used in pollination in the prior art.
The present invention provides the use of a plant immune protein or a composition comprising a plant immune protein to promote elongation of plant pollen tubes. The invention adopts plant immunity protein as the auxiliary agent for pollen tube growth, can accelerate the elongation of pollen tube, shortens fertilization time and improves fruit setting rate.
The invention provides the use of a plant immunity protein or a composition containing a plant immunity protein for increasing the elongation rate of a plant pollen tube. After CN115843823A, the invention unexpectedly discovers that plant immunity protein with a certain concentration can also promote the elongation of plant pollen tubes and promote the elongation rate of the plant pollen tubes, namely, the invention can simultaneously promote the elongation rate of the pollen tubes, improve the germination activity of pollen, improve the activity of pollen and improve the fruit setting rate. The invention provides a new application of plant immunity protein in promoting germination of plant pollen.
According to the use, the composition is a pollination aid comprising a plant immune protein.
Preferably, the pollination aid can be a powdered solid or a liquid.
According to the application, the final content of the plant immunity protein in the application of the auxiliary agent for pollination is 0.02-3.3 g/L.
According to the application, the final content of the plant immunity protein in the application of the auxiliary agent for pollination is 0.05-3.2 g/L.
Preferably, the final content of the plant immunity protein is 0.5-3.0 g/L when the pollination aid is used in specific application.
In some embodiments of the invention, the final content of plant immunity protein in the pollination aid application is 0.2-3.0 g/L.
In some embodiments of the invention, the final content of plant immunity protein in the pollination aid application is 0.5-2.9 g/L.
According to the application, the pollination aid contains plant immunity proteins and also contains substances required by pollen germination, such as white granulated sugar, boric acid, calcium chloride and the like.
According to the application, the plant immunity protein, white sugar, boric acid and calcium chloride are mixed to obtain the pollination aid.
Depending on the application, the concentration of each substance in the final pollination liquid is: 0.02-3.3g/L plant immunity protein, 80-130g/L white sugar, 0.08-0.12g/L boric acid and 0.08-0.12g/L calcium chloride.
Preferably, the concentration of each substance in the final pollination liquid is: 0.05-3.2g/L plant immunity protein, 80-120g/L white sugar, 0.08-0.12g/L boric acid and 0.08-0.12g/L calcium chloride.
Preferably, the pollination aid is mixed with water to obtain a mixed solution, and pollen is put into the mixed solution to be uniformly mixed to obtain liquid pollen.
Preferably, the pollen is that of a plant requiring cross pollination.
Depending on the application, the pollination liquid is sprayed onto the open plant stigma. Thereby accelerating the elongation of the pollen tube, shortening the time for the pollen tube to reach the ovary and accelerating the fertilization of the flower.
The pollination aid provided by the invention has the function of promoting the elongation of pollen tubes. The special expression can promote the rate of the pollen tube extension, thereby achieving the purposes of improving fruit setting rate, variety diversity, variety quality and the like in agricultural production.
The invention also provides an effect of the pollination aid in promoting the germination of pollen tubes.
The invention also provides application of the pollination aid in improving the fruit setting rate of cross-pollinated crops and improving the crop yield.
According to the application, the plant immunity protein is extracted from Botrytis cinerea, alternaria alternata and Fusarium.
According to the application, the plant pollen is plant pollen such as apricot pollen, pear pollen, apple pollen or almond pollen.
The invention has the beneficial effects that:
compared with the prior art, the invention takes a certain amount of plant immunity protein as the promoter for germination and elongation of the plant pollen tube, has obvious germination and elongation effects on the plant pollen tube, and has the advantages of low cost, safety, no toxicity and simple preparation. The plant pollen tube elongation promoter can also improve plant disease resistance, promote stem and leaf growth and improve chlorophyll content.
Drawings
In order to more clearly illustrate the invention or the technical solutions of the prior art, the following description will briefly introduce the drawings that are needed in the embodiments or the prior art description, and it is obvious that the drawings in the following description are some embodiments of the invention.
FIG. 1 effect of different concentrations of plant immunity proteins of the invention on apricot pollen tube length.
FIG. 2 effects of different concentrations of plant immunoproteins of the invention on pear pollen tube elongation rate.
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the present invention more apparent, the technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings, and it is apparent that the described embodiments are some embodiments of the present invention, not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
The plant immunity protein used in the invention. The plant immunity protein is a novel structural protein separated and extracted from microorganism by biotechnology. The plant immunity protein used in the invention is extracted from Botrytis cinerea, alternaria alternata and Fusarium.
The white sugar, boric acid and calcium chloride used in the present invention are all commercially available.
EXAMPLE 1 Effect of different concentrations of plant immune proteins on apricot pollen tube Length
The embodiment provides a preparation method of apricot liquid pollen, which comprises the following steps:
the pollen protein solution was prepared by weighing 0.05 g, 0.1 g, 0.2 g, 0.5 g, 1.5 g, 3.0 g, 3.2g, 3.5 g, 4.0 g and 4.5 g plant immunity proteins, respectively, and formulating with 100.0 g white sugar, 0.1 g boric acid, 0.1 g calcium chloride and 1.0L purified water into 0.05 g/L (treatment group 1), 0.1 g/L (treatment group 2), 0.2 g/L (treatment group 3), 0.5 g/L (treatment group 4), 1.5 g/L (treatment group 5), 3.0 g/L (treatment group 6), 3.2g/L (treatment group 7), 3.5 g/L (treatment group 8), 4.0 g/L (treatment group 9) and 4.5 g/L (treatment group 10), respectively, and adding 2. 2.0 g of pollen protein solution into each treatment group to obtain a pollen protein solution with different pollen concentration. The protein pollen aqueous solutions are all prepared in the prior art and are respectively added into solid culture media for pollen in-vitro germination.
The control group was pollen aqueous solution without elongation aid (plant immunity protein). Microscopic observation after 12 hours of illumination at 27℃as shown in FIG. 1, the pollen lengths of treatment groups 1, 2, 3, 4, 5, 6, and 7 were significantly increased compared to the control group, and the pollen tube lengths were 649.42 μm, 715.34 μm, 735.87 μm, 781.22 μm, 842.56 μm, 818.44 μm, and 653.25 μm, respectively; the treated groups 8, 9, 10 had significantly reduced pollen tube lengths as compared to the control group, 521.47 μm, 478.57 μm and 465.37 μm respectively.
Example 2 Effect of different concentrations of plant immunoprotein on pear pollen tube elongation
This example prepared pear liquid pollen containing different levels of plant immunity proteins, with the difference that:
in the embodiment, pear liquid pollen without plant immunity protein is used as a control group; pear liquid pollen with the content of plant immunity protein of 1.0 g/L is used as a treatment group 1; pear liquid pollen with the content of plant immunity protein of 2.0 g/L is used as a treatment group 2; pear liquid pollen with the content of plant immunity protein of 3.0 g/L is used as a treatment group 3. The length of the liquid pollen tube was measured 1 hour, 2 hours, 3 hours, and 4 hours after completion of the preparation of liquid pollen, respectively (fig. 2).
As a result, it was found that the pollen tube lengths of the control groups were 74.28 μm,121.08 μm,198.42 μm and 318.4 μm at 1 hour, 2 hours, 3 hours and 4 hours after completion of the preparation of liquid pollen, respectively; pollen tube lengths of treatment group 1 were 104.05 μm, 179.68 μm, 235.42 μm and 415.04 μm, respectively; pollen tube lengths of treatment group 2 were 129.25 μm, 217.44 μm, 275.1 μm and 478.16 μm, respectively; the pollen tube lengths of treatment group 3 were 172.28 μm, 259.48 μm, 383.24 μm and 519.14 μm, respectively. The treatment groups 1, 2 and 3 respectively have the pollen tube elongation rates of 1 hour, 2 hours, 3 hours and 4 hours after the preparation of the liquid pollen, which are higher than the pollen tube elongation rates of the control group at 1 hour, 2 hours, 3 hours and 4 hours after the preparation of the liquid pollen, which indicates that the plant immunity protein used this time is used as an auxiliary agent, and the liquid pollen tube elongation rate is improved.
Finally, it should be noted that: the above embodiments are only for illustrating the technical solution of the present invention, and are not limiting; although the invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical scheme described in the foregoing embodiments can be modified or some technical features thereof can be replaced by equivalents; such modifications and substitutions do not depart from the spirit and scope of the technical solutions of the embodiments of the present invention.
Claims (10)
1. Use of a plant immunity protein or a composition comprising a plant immunity protein for promoting plant pollen tube elongation.
2. Use of a plant immunity protein or a composition comprising a plant immunity protein for increasing the elongation rate of a plant pollen tube.
3. The use according to claim 1 or 2, wherein the composition is a pollination aid comprising a plant immune protein.
4. The use according to claim 3, wherein the final content of plant immunoprotein in the pollination aid application is 0.02-3.3 g/L.
5. The use according to claim 3, wherein the pollination aid comprises plant immunity proteins, white sugar, boric acid and calcium chloride.
6. The use according to claim 5, characterized in that the concentration of each substance in the final pollination liquid is 0.02-3.3g/L plant immunity protein, 80-130g/L white sugar, 0.08-0.12g/L boric acid and 0.08-0.12g/L calcium chloride.
7. The use according to claim 6, characterized in that the concentration of each substance in the final pollination liquid is: 0.05-3.2g/L plant immunity protein, 80-120g/L white sugar, 0.08-0.12g/L boric acid and 0.08-0.12g/L calcium chloride.
8. The use according to any one of claims 6 to 7, characterized in that the pollination liquid is sprayed on the open plant stigma.
9. The use according to claim 1 or 2, wherein the plant immune protein is extracted from botrytis, alternaria and fusarium.
10. The use according to claim 1 or 2, wherein the plant pollen is apricot pollen, pear pollen, apple pollen or badam pollen.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310738988.0A CN116473082A (en) | 2023-06-21 | 2023-06-21 | Auxiliary agent for accelerating elongation of pollen tube and formula thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202310738988.0A CN116473082A (en) | 2023-06-21 | 2023-06-21 | Auxiliary agent for accelerating elongation of pollen tube and formula thereof |
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| CN116473082A true CN116473082A (en) | 2023-07-25 |
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