CN1164336C - Biological dressing and its preparing method - Google Patents
Biological dressing and its preparing method Download PDFInfo
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- CN1164336C CN1164336C CNB021105995A CN02110599A CN1164336C CN 1164336 C CN1164336 C CN 1164336C CN B021105995 A CNB021105995 A CN B021105995A CN 02110599 A CN02110599 A CN 02110599A CN 1164336 C CN1164336 C CN 1164336C
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- enzyme
- chitosan
- biological
- wounds
- biological dressing
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Abstract
The present invention discloses a biological dressing and a preparing method thereof. The biological dressing comprises chitosan, acetic acid and a biological enzyme, preferably the FE composite enzyme. The preparing method of the biological dressing comprises the steps of dissolving the chitosan by water and the acetic acid, adding the biological enzyme, stirring and filtering. The biological dressing can be used for treating wounds of human bodies. The present invention degrades into partial oligosaccharide during storage by the chitosan and the FE composite enzyme bactericide so that the FE composite enzyme bactericide can quickly kill germs carried by wounds when the dressing is applied to the wounds, and the oligosaccharide can kill germs and can be absorbed by human bodies under the action of lysozymes in liquid exuding from wounds to form a wound healing agent. The rest parts which do not degrade are naturally formed into films on wounds to form natural artificial skin barriers to prevent germs intruding. The present invention has no toxicity, no harm, no sensitization or stimulation to human bodies.
Description
Technical field
The present invention relates to a kind of medicine for the treatment of wound, scald, the particularly a kind of treatment wound of chitosan, natural biological medicine of scald and preparation method thereof of containing.
Background technology
After human external is subjected to wound, must treat timely, as medicine, so that wound heals as early as possible in site of injury coating antiinflammatory, granulation promoting.Traditional method all reaches antibiotic, germ-resistant purpose by chemicals or antibiotic, thereby is difficult to avoid human body skin is produced bad stimulation and Resistant strain.Chinese patent application numbers 94107473.0 discloses the technology of a kind of denomination of invention for " preparation of chitosan iodine wound and burns dressing ", this patent by chitosan as slow releasing agent, to reach the purpose of better anti-bacterial effect and accelerating wound.Its weak point is: this technology is not still broken away from traditional mode, promptly reach antibiotic, germ-resistant purpose by chemicals or antibiotic, thereby be difficult to avoid human body skin is produced bad stimulation and Resistant strain, and be applied to wound especially the compress on scalded skin surface be easy to cause wound breathability difference and causing and the wound adhesion, for removing the misery that new wound surface that the wound adhesion causes can delay the healing of wound again and add the heavy patient.
Summary of the invention
The technical issues that need to address of the present invention are biological dressings and preparation method thereof, to overcome the deficiency that prior art exists, need not any chemicals and antibiotic prerequisite under, can reach simultaneously quick sterilization, lasting sterilization, accelerating wound again, reduce patient's the painful purpose of changing dressings to greatest extent.
Technical scheme of the present invention:
Biological dressing of the present invention is a kind of compositions, and its component and weight percent content comprise:
Chitosan (chitosan-containing enzyme) 0.1～99.98%
Acetic acid 0.01～10%
Enzyme 0.01～50%
Water 0～99.88%
Above-mentioned enzyme can be a kind of in staphylococcus lysozyme, endogenous or exogenous lysozyme, cellulase, glucose enzyme, protease or the lipase or their compound enzyme, preferably FE compound enzyme.
Said chitosan is the derivant of chitin after refining, deacetylated, and its technology of preparing is reported in about " the preferred research of chitosan production technology " document in that Marine University Of Zhanjiang's journal the 17th volume the 2nd is interim, is summarized as follows:
Chitin is immersed in the hydrochloric acid solution of 1.9mol/L, is warming up to 65 ℃～70 ℃ and kept 1～1.5 hour, when it reaches the degraded critical point, take out.After the flush away acid solution, immerse in the sodium hydroxide solution of 2.5mol/L, be warming up to 70 ℃～75 ℃ and kept 3 hours.Flush away alkali liquor immerses in the sodium hydroxide solution of 10.0～12.5mol/L, is warming up to 65 ℃～70 ℃ and keeps 2.5～3 hours, and behind the flush away alkali liquor, reuse distilled water drip washing 2～3 times and pressure sterilizing can obtain the chitosan of being addressed;
Said FE compound enzyme is a kind of lysozyme, and the model that can preferably adopt Gaoke Bio-Engineering Co Ltd, Shanghai to produce is the product of " FE of hectogram Rui Gao section compound enzyme ".
Preferred ingredients and weight percent content comprise:
Chitosan (chitosan-containing enzyme) 1～99.4%
Acetic acid 0.5～2.0%
FE compound enzyme (3u/mL of active unit) 0.1～33%
Preferred ingredients and weight percent content comprise:
Chitosan (chitosan-containing enzyme) 1.2～98.2%
Acetic acid 0.8～1.5%
FE compound enzyme (3u/mL of active unit) 1～16%.
Above-mentioned biological dressing prepares by the following method:
After dissolving chitosan in proportion with distilled water, acetic acid, under 50 ℃～55 ℃ temperature, add enzyme in proportion, stir evenly, filter, can obtain said biological dressing.
Above-mentioned biological dressing can be used for treating the wound of human body, and using method is as follows:
The present invention adopts full exposure or semi-exposure method, cleans wound surface routinely, and dressing evenly is coated in the human body wound, makes its drying and forming-film.Available in case of necessity antiseptic gauze wrapping applies 2～3 every day.
The present invention is the bactericidal properties according to dressing, the needs of film property, the FE compound enzyme antibacterial that utilizes chitosan and allocate into self is degraded into the part oligosaccharide in storage process, FE compound enzyme antibacterial can be killed the pathogenic bacteria of wound fast when making dressing be coated to wound, and wherein oligosaccharide part is except sterilization functions, and again can be in wound fluid being absorbed by the body under the effect of lysozyme becomes Wound-healing agent.All the other parts of not degrading are in wound nature film forming, form natural artificial skin barrier and stop the pathogenic bacteria invasion, because itself and human body affinity are good, and be good to the transudate absorbability, in wound fluid, can further degrade again under the effect of lysozyme and continue to generate oligosaccharide.So, under the protection of dressing membrane, formed the optimum ecological circulation that a natural human body and beneficial microbe tackle pathogenic bacteria jointly.Because the pliability of dressing membrane is suitable, smooth in the wound face covering, not only can ease pain, and the epidermis that generates in the back that is absorbed by the body is smooth, need not peel off after the wound healing, can repeatedly repeat coating.
By above-mentioned disclosed technical scheme as seen:
1, the present invention is nontoxic to human body, no sensitization, stimulation.
2, stronger bacteriostasis is arranged, particularly in storage process, its not anti-reflection of bacteriostasis increases.
3, utilized the Degradation of enzyme, more helped sterilizing and oligosaccharide composition that human body skin absorbs.
4, reduced cost effectively, helped towards masses.
5, made full use of the FE compound enzyme to golden staphylococci and the oligosaccharide effective killing action to bacillus pyocyaneus, both have complementary advantages, and are the Resistant strain of present golden staphylococci and these two scalded patient formidable enemies' of bacillus pyocyaneus jinx.
6, help the healing of wound, under the effect of chitosan a large amount of lysozyme in wound fluid, further degrade and become the utilization that is absorbed by the body behind the monomer, thereby accelerated wound healing.
7, alleviate the cicatrix of wound because chitosan forms a kind of protecting film in wound surface, its film have air permeability and good and with the human body excellent biological compatibility; its pliability is suitable; combine smoothly with wound face, the epidermis that generates in the back that is absorbed by the body is smooth, can obviously alleviate cicatrix.
8, alleviate patient's the misery of changing dressings,, can also repeatedly repeat coating because chitosan film can be by human body degraded and absorbed effectively at human body surface, so need not peel off after the wound healing.
Specific implementation method
Embodiment 1～9
Prepare biological dressing according to following ratio:
Embodiment 123456789
Chitosan 1.2 1.3 1.4 1.7 1.8 1.9 2.0 2.1 2.1
FE compound enzyme 16 16 16 16 16 16 16 16 11
Its inhibition zone data are as follows:
Embodiment escherichia coli bacillus pyocyaneus golden staphylococci
1 18.90 15.84 26.54
2 19.62 16.08 26.30
3 15.92 12.56 23.40
4 15.58 12.42 24.38
5 15.60 12.42 24.52
6 16.28 11.54 24.52
7 16.28 10.70 24.52
8 16.94 13.12 24.72
9 16.52 11.48 23.22
The sample of embodiment 3 is as follows in the antibacterial data contrast of 54 ℃ of heat after 2 weeks of storage:
Escherichia coli bacillus pyocyaneus golden staphylococci
After heat is stored up before the hot storage after heat storage before the preceding heat storage of the heat storage after heat storage
15.92 19.60 12.56 15.20 23.40 25.16
Claims (3)
1. a biological dressing is characterized in that, this biological dressing is made up of the component of following weight percent content:
The chitosan 0.1～99.98% of chitosan-containing enzyme
Acetic acid 0.01～10%
Enzyme 0.01～50%
Water 0～99.88%
Said enzyme is a kind of in staphylococcus lysozyme, interior originality or the exogenous lysozyme or their compound enzyme or FE compound enzyme.
2. biological dressing as claimed in claim 1 is characterized in that, said FE compound enzyme is that active unit is the FE compound enzyme of 3u/mL.
3. the preparation method of biological dressing as claimed in claim 1 is characterized in that, this method comprises the steps: to add enzyme with distilled water, acetate dissolution chitosan, stirs evenly, filters, and can obtain said biological dressing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB021105995A CN1164336C (en) | 2002-01-21 | 2002-01-21 | Biological dressing and its preparing method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB021105995A CN1164336C (en) | 2002-01-21 | 2002-01-21 | Biological dressing and its preparing method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1364646A CN1364646A (en) | 2002-08-21 |
| CN1164336C true CN1164336C (en) | 2004-09-01 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB021105995A Expired - Fee Related CN1164336C (en) | 2002-01-21 | 2002-01-21 | Biological dressing and its preparing method |
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| Country | Link |
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| CN (1) | CN1164336C (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100453121C (en) * | 2002-11-12 | 2009-01-21 | 吴奕光 | Medical dressing and its producing method |
| CN100353932C (en) * | 2004-03-22 | 2007-12-12 | 集美大学 | Composite type superoxide anion free radical scavenger and its preparation method |
| CN103285418A (en) * | 2013-06-17 | 2013-09-11 | 上海微纳科技有限公司 | Novel biological antibacterial dressing aerosol based on large-area burn wound |
| CN106822880A (en) * | 2016-12-30 | 2017-06-13 | 江阴市本特塞缪森生命科学研究院有限公司 | A kind of antimicrobial compositions and its application |
| CN109529099B (en) * | 2018-11-27 | 2022-01-14 | 浙江海洋大学 | Double-network chitosan enzyme-loaded hydrogel dressing based on three-dimensional printing technology and preparation method thereof |
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2002
- 2002-01-21 CN CNB021105995A patent/CN1164336C/en not_active Expired - Fee Related
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| CN1364646A (en) | 2002-08-21 |
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