CN116425320A - ISSA PGPR combined plant remediation technology for eutrophic river and lake - Google Patents
ISSA PGPR combined plant remediation technology for eutrophic river and lake Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/348—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
- A01G31/02—Special apparatus therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/04—Arrangements for treating water specially adapted to receptacles for live fish
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
- C02F3/327—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae characterised by animals and plants
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/007—Contaminated open waterways, rivers, lakes or ponds
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2305/00—Use of specific compounds during water treatment
- C02F2305/06—Nutrients for stimulating the growth of microorganisms
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A20/00—Water conservation; Efficient water supply; Efficient water use
- Y02A20/40—Protecting water resources
- Y02A20/402—River restoration
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Abstract
The invention relates to the field of ecological restoration, and particularly discloses a technology for restoring eutrophic rivers and lakes by ISSAPGPR combined plants; the invention uses ISSAPGPR in-situ ecological restoration technology to selectively activate indigenous PGPR (PlantGrowth PromotingRhizobacteria) microorganisms in water environment by enzyme, and make them rapidly reproduce, and reduce eutrophic substances in water body by aerobic denitrification and synchronous nitrification denitrification nitrogen removal of partial PGPR microorganisms; through the growth promoting effect of animals and plants, a high-efficiency food chain of 'big fish eating small fish and small fish eating shrimp' is established, the in-situ repair and the improvement of an ecological system are realized, the self-cleaning function of the ecological system is greatly increased, the purposes of in-situ treatment and utilization of pollutants and rapid improvement of water quality are achieved, and finally the ecological system of a water body is balanced.
Description
Technical Field
The invention belongs to the technical field of ecological restoration, and particularly relates to a technology for restoring eutrophic rivers and lakes by combining ISSA PGPR with plants.
Background
At present, the most common methods in river and lake treatment and repair at home and abroad mainly comprise a physical method, a chemical method, a biological method and the like. Among them, the bioremediation technology has a great number of advantages of small investment, high efficiency, and the like, and has simple operation and good potential.
In the Chinese patent with publication number of CN108275832B, a method for repairing river black and odorous water and eutrophic lake water by a combined fluidized bed is disclosed, which comprises the following steps: the method can effectively repair black and odorous water bodies of river channels and eutrophic lakes and restore better water quality through the treatment of a primary oxygen/air source ozone micro-nano pipeline fluidized bed, a multi-stage A/O mud membrane co-metabolism in-situ micro-oxygen particle biological fluidized bed, a secondary O3 micro-nano molecular sieve fluidized bed, a photocatalytic reactor and a filtering fluidized bed.
The applicant finds that in the application process, the river restoration combined structure in the application relates to a plurality of treatment systems and treatment methods, wherein the chemical flocculation method needs to use a plurality of compounds and operate through a plurality of procedures, the water body after the chemical purification procedure also needs to be subjected to physical purification steps such as scum collection, photocatalysis and the like, and the purification procedures are accumulated together, and can play a good role in a short time, but the water body is excessively purified to cause that the ecological balance of the water body cannot be maintained for a long time, water body eutrophication rebound is extremely easy to occur, and the whole equipment has large construction investment and is not easy to operate and can not be used in a large-scale water body environment for a long time.
Disclosure of Invention
The invention aims to provide a technology for repairing eutrophic rivers and lakes by combining ISSA PGPR with plants, so as to solve the problems in the prior art.
In order to achieve the above purpose, the present invention provides the following technical solutions:
a technique for repairing eutrophic rivers and lakes by combining ISSA PGPR with plants, which comprises the following steps:
s1, constructing an ecological restoration system according to PGPR microorganisms of the relevant river and lake;
s2, arranging an ecological restoration system in a relevant area of a river and a lake, and conveying partial water in a water area to the ecological restoration system through a submersible pump;
s3, a large number of PGPR microorganisms are propagated and grown in the ecological restoration system, and then the large number of activated PGPR microorganisms automatically flow back into the water body from the equipment under the carrying of water;
s4, the effluent rich in PGPR microorganisms is diffused into the whole water body under the actions of water flow in the water area, waves, self swimming and the like;
s5, the PGPR microorganisms are metabolized and propagated by taking pollution sources as food sources, PGPR microorganism metabolites are taken as nutrient substances absorbed by plankton and water plants in water, plant growth is promoted by various microorganism secondary metabolism wastes, aquatic plants provide living places and foods for microorganisms and aquatic animals, benign ecological structure food chains are established, and water quality is continuously improved.
Preferably, in S1, the ecological restoration system includes two parts, an ecological restoration agent and an artificial reaction platform, the ecological restoration agent is a nano-scale nutrition granule capable of selectively activating PGPR, and the artificial reaction platform is internally provided with the ecological restoration agent for propagating PGPR microorganisms and delivering the PGPR microorganisms to an external water environment for restoration.
Preferably, in S1, the ecological restoration agent contains various nutrients including carbon sources, enzymes, trace elements and other carriers for activating PGPR, and is uniform particles prepared by a micro-coating technology, and the ecological restoration agent can continuously and slowly supply various nutrients to PGPR microorganisms in the environment.
Preferably, in S1, the artificial reaction platform is capable of providing a semi-closed PGPR microorganism activation environment, and has a function of automatically sending PGPR microorganisms in an external environment into the platform for activation, and sending the activated PGPR microorganisms back into an external water environment.
Preferably, in S1, the artificial reaction platform may be set as water surface equipment or shore equipment according to the water environment and the condition of the land, where the water surface artificial reaction platform is composed of a foaming buoyancy tank, a reaction tank, an information feedback device, and the like, and may float along with the fluctuation of the water level, the shore equipment is fixed on the shore near the center of the water area, the polluted water is pumped into the large-sized bucket containing the ecological restoration agent by the water pump, and after circulation, the polluted water is discharged into the water body at a certain flow rate, so as to construct a self-circulation process of water flow from the external environment to the inside of the system and then to the external environment.
Preferably, the purification function of the PGPR microorganism on the eutrophic water body is mainly divided into three types: aerobic denitrification, phosphorus accumulating bacteria, food chain transfer nitrogen and phosphorus and biological dredging, wherein the aerobic denitrification is aimed at reducing the concentration of ammonia nitrogen in a water body, the phosphorus accumulating bacteria and the food chain transfer nitrogen and phosphorus are aimed at reducing eutrophic substances such as nitrogen and phosphorus in the water body, and the biological dredging can be used for rapidly decomposing and removing organic matters in a water area.
Compared with the prior art, the invention has the beneficial effects that:
the invention uses ISSA PGPR in-situ ecological restoration technology to selectively activate indigenous PGPR (Plant Growth Promoting Rhizobacteria) microorganisms in water environment by enzyme, and make them rapidly reproduce, and reduce eutrophic substances in water body by aerobic denitrification and synchronous nitrification denitrification nitrogen removal of partial PGPR microorganisms; through the growth promoting effect of animals and plants, a high-efficiency food chain of 'big fish eating small fish and small fish eating shrimp' is established, the in-situ repair and the improvement of an ecological system are realized, the self-cleaning function of the ecological system is greatly increased, the purposes of in-situ treatment and utilization of pollutants and rapid improvement of water quality are achieved, and finally the ecological system of a water body is balanced.
Drawings
FIG. 1 is a water quality improvement technical roadmap of the invention;
FIG. 2 is a list of strains capable of aerobic denitrification according to the invention;
FIG. 3 is an electron transfer model of the aerobic denitrification process of the invention;
FIG. 4 is a schematic diagram of the nitrogen and phosphorus removal of the food chain of the present invention;
FIG. 5 is a schematic illustration of biosludging according to the present invention;
fig. 6 is a schematic diagram of application of PGPR ecological restoration technology of the present invention.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Plant growth-promoting microorganism (PGPR) refers to a general term for microorganisms that attach to the surface of plant rhizosphere and grow in the plant rhizosphere soil microenvironment to significantly promote plant growth. PGPR has been widely used in ecological agriculture and soil remediation, both for disease prevention and for yield increase. The PGPR can create a good rhizosphere ecological environment, inhibit the occurrence of plant diseases and insect pests and reduce the use of chemical fertilizers and pesticides. In the ecological system of heavy metal contaminated soil, PGPR produces a lot of substances beneficial to plant growth, which can help host plants adapt to unfavorable soil conditions, and plays an important role in improving plant heavy metal resistance, promoting plant growth and affecting the absorption, transportation and enrichment of heavy metals. Meanwhile, PGPR can improve the degradation efficiency of hydrocarbon in soil, and has good repairing effect on petroleum-polluted saline-alkali soil. The existing research finds that PGPR bacteria also exist in water environment, and a plurality of PGPR microorganisms are found in the polluted water body, and the microorganisms have good effects in ecological restoration and water quality improvement.
When the amount of pollutants in the polluted water exceeds the environmental load or the nutrition condition is not proper, the population and the number of PGPR microorganisms are not very large, and the effect is limited. If appropriate conditions are given to the microorganism such as: the growth and propagation of microorganisms are very fast, such as culture medium, pH, temperature and the like with optimal nutrition element proportion; however, in the open system of nature, it is impossible to control the pH, temperature, etc. of the water body, but it is possible to provide nutrient conditions necessary for the growth and reproduction of the PGPR microorganism to be beneficial in the natural environment to promote the reproduction and growth of the PGPR microorganism
Referring to fig. 1-6, a technique for repairing eutrophic rivers and lakes by using ISSA PGPR in combination with plants includes the following steps:
s1, constructing an ecological restoration system according to PGPR microorganisms of the relevant river and lake;
s2, arranging an ecological restoration system in a relevant area of a river and a lake, and conveying partial water in a water area to the ecological restoration system through a submersible pump;
s3, a large number of PGPR microorganisms are propagated and grown in the ecological restoration system, and then the large number of activated PGPR microorganisms automatically flow back into the water body from the equipment under the carrying of water;
s4, the effluent rich in PGPR microorganisms is diffused into the whole water body under the actions of water flow in the water area, waves, self swimming and the like;
s5, the PGPR microorganisms are metabolized and propagated by taking pollution sources as food sources, PGPR microorganism metabolites are taken as nutrient substances absorbed by plankton and water plants in water, plant growth is promoted by various microorganism secondary metabolism wastes, aquatic plants provide living places and foods for microorganisms and aquatic animals, benign ecological structure food chains are established, and water quality is continuously improved.
In S1, the ecological restoration system comprises an ecological restoration agent and an artificial reaction platform, wherein the ecological restoration agent is a nano-scale nutrition granule capable of selectively activating PGPR, and the artificial reaction platform is internally provided with the ecological restoration agent for propagating PGPR microorganisms and conveying the PGPR microorganisms to an external water environment for restoration.
In S1, the ecological restoration agent contains various nutrients for activating PGPR including carbon source, enzyme, trace elements and other carriers, the ecological restoration agent is uniform particles prepared by adopting a micro-coating technology, and can continuously and slowly supply various nutrients to PGPR microorganisms in the environment, so that the PGPR microorganisms in the water body are continuously activated and rapidly propagated, the total number of the PGPR microorganisms in the activated water body is about 100 times of the original number, and the number of the PGPR microorganisms can reach 10 5 ～10 8 cfu/ml, and the ecological restoration agent can be completely utilized by microorganisms without secondary pollution.
In S1, the artificial reaction platform can provide a semi-closed PGPR microorganism activation environment and has the functions of automatically sending the PGPR microorganisms in the external environment into the platform for activation and sending the activated PGPR microorganisms back into the external water environment.
The artificial reaction platform has the following characteristics:
(1) The semi-closed internal environment can be provided, an ecological restoration agent can be placed, and a PGPR activation platform is established;
(2) The self-circulation process of water flow from the external environment to the inside of the system to the external environment can be realized, so that the aim that PGPR in the water body is continuously fed into the ecological restoration system for activation and the activated PGPR is continuously thrown into the water body is fulfilled;
(3) The artificial reaction platform can be set as water surface equipment or on-shore equipment according to the water environment and the standing condition. The water surface artificial reaction platform consists of a foaming buoyancy tank, a reaction tank, an information feedback device and the like, and can float along with the fluctuation of the water level; the on-shore equipment is fixed on the shore near the center of the water area, the polluted water body is pumped into a large-scale water barrel filled with the ecological restoration agent through a water pump, and the water body is discharged into the water body at a certain flow after circulation, so that a self-circulation process of water flow from the external environment to the inside of the system and then to the external environment is constructed.
In S1, the artificial reaction platform can be set as water surface equipment or on-shore equipment according to the water environment and the standing condition, the water surface artificial reaction platform consists of a foaming buoyancy tank, a reaction tank, an information feedback device and the like, can float along with the fluctuation of the water level, the on-shore equipment is fixed on the shore near the center of a water area, polluted water is pumped into a large-sized bucket filled with an ecological restoration agent through a water pump, and is discharged into the water body at a certain flow rate after circulation, so that a self-circulation process of water flow from the external environment to the inside of the system and then to the external environment is constructed.
The purification function of the PGPR microorganisms on eutrophic water is mainly divided into three types: aerobic denitrification, phosphorus accumulating bacteria, food chain transfer nitrogen and phosphorus and biological dredging, wherein the aerobic denitrification is aimed at reducing the concentration of ammonia nitrogen in a water body, the phosphorus accumulating bacteria and the food chain transfer nitrogen and phosphorus are aimed at reducing eutrophic substances such as nitrogen and phosphorus in the water body, and the biological dredging can be used for rapidly decomposing and removing organic matters in a water area.
Aerobic denitrification nitrogen removal: by installing an ecological restoration system, microorganisms with aerobic denitrification in PGPR are selectively activated, and aerobic denitrification bacteria are also heterotrophic nitrifying bacteria, so that the nitrification and denitrification can be simultaneously carried out under the aerobic condition. The nitrified product can be directly used as a substrate for denitrification, so that the inhibition of accumulation of nitric acid and nitrous acid to the nitrification reaction is avoided, and the nitrification-denitrification process is accelerated, thereby achieving the purpose of rapidly reducing the ammonia nitrogen concentration in the water body.
Phosphorus accumulating bacteria and food chain transfer nitrogen and phosphorus: through the selective activation of microorganisms such as rhodopirilaceae (rhodopirilaceae), bacillus, alcaligenes (Alcaligenes), phosphorus accumulating bacteria and the like, wherein the phosphorus accumulating bacteria are propagated in a large quantity in an oxygen consumption environment, and absorb soluble phosphorus in a water body and transfer the soluble phosphorus into the body, so that the phosphorus content in the water body is rapidly reduced; the microorganisms such as bacillus and alcaligenes which are propagated in a large quantity convert rich nutrient substances such as nitrogen and phosphorus in the water body into nutrient substances which can be absorbed by plankton and water body plants, so that the number of rotifers, cladocera and copepods in the water body is obviously increased, and the total number of plankton can be increased by 1.5-3 times. Meanwhile, secondary metabolites generated by the microorganisms in the propagation and growth processes can promote the growth of plants, aquatic plants provide living places and foods for the microorganisms and aquatic animals, the ecological structure is enriched, a benign food chain for large fishes to eat small fishes and small fishes to eat dried shrimps is established, the concentration of nitrogen and phosphorus in a water body is further reduced through the efficient food chain, the self-cleaning capacity of the water body is improved, and the effect of continuously improving the water quality is achieved.
Biosolids (the decomposition of organic matter in sludge): under the condition of more organic matter content in the restored water body sediment, an ecological restoration system is arranged at a proper position of a water area to selectively activate microorganisms with rapid decomposition of organic matters in the PGPR. By selectively activating microorganisms such as Pseudomonas (Pseudomonas), micrococcus (Micrococcus), flavobacterium (Chryseobacterium), acinetobacter (Acinetobacter), etc., organic matter can be rapidly decomposed. Through ecological restoration for one year, the removal rate of organic matters in the sediment can reach 50% -70%, and the content of organic matters in the sludge is greatly reduced, so that the purpose of biological desilting is achieved.
From the above, users can transport part of water in a water area to an ecological restoration system through a submersible pump, a large number of primary PGPR microorganisms in part of water are propagated and activated in the ecological restoration system, then a large number of activated PGPR microorganisms automatically flow back to the water body from equipment under the carrying of water, at the moment, the water rich in microorganisms diffuses the PGPR microorganisms into the whole water body under the actions of water flow, waves, self swimming and the like in the water area, and the PGPR microorganisms metabolize and propagate in the external water body by taking pollutants as food sources, and meanwhile participate in food chains in the external water body to balance the eutrophicated water body.
The ISSA PGPR ecological restoration technology in the application can be widely applied to fresh water and sea water conditions. But attention should be paid to the suitable conditions for use of the present technology: the pollution source needs to be controlled and reduced; has synergistic effect with aeration and aquatic animal and plant technology; suggesting revetments and rivers and lakes to adopt ecological forms; and the use of bactericidal agents and antibiotics is strictly prohibited to prevent killing or inhibiting microorganisms.
Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (6)
1. A technique for repairing eutrophic rivers and lakes by combining isspgpr with plants, which is characterized by comprising the following steps:
s1, constructing an ecological restoration system according to PGPR microorganisms of the relevant river and lake;
s2, arranging an ecological restoration system in a relevant area of a river and a lake, and conveying partial water in a water area to the ecological restoration system through a submersible pump;
s3, a large number of PGPR microorganisms are propagated and grown in the ecological restoration system, and then the large number of activated PGPR microorganisms automatically flow back into the water body from the equipment under the carrying of water;
s4, the effluent rich in PGPR microorganisms is diffused into the whole water body under the actions of water flow in the water area, waves, self swimming and the like;
s5, the PGPR microorganisms are metabolized and propagated by taking pollution sources as food sources, PGPR microorganism metabolites are taken as nutrient substances absorbed by plankton and water plants in water, plant growth is promoted by various microorganism secondary metabolism wastes, aquatic plants provide living places and foods for microorganisms and aquatic animals, benign ecological structure food chains are established, and water quality is continuously improved.
2. The technique for combined phytoremediation of an isapgpr-enriched river or lake according to claim 1, wherein: in S1, the ecological restoration system comprises an ecological restoration agent and an artificial reaction platform, wherein the ecological restoration agent is a nano-scale nutrition granule capable of selectively activating PGPR, and the artificial reaction platform is internally provided with the ecological restoration agent and is used for propagating PGPR microorganisms and conveying the PGPR microorganisms to an external water environment for restoration.
3. The technique for combined phytoremediation of an isapgpr-enriched river or lake according to claim 1, wherein: in S1, the ecological restoration agent contains various nutrients including carbon sources, enzymes, trace elements and other carriers for activating PGPR, is uniform particles prepared by adopting a micro-coating technology, and can continuously and slowly supply various nutrients to PGPR microorganisms in the environment.
4. The technique for combined phytoremediation of an isapgpr-enriched river or lake according to claim 1, wherein: in S1, the artificial reaction platform can provide a semi-closed PGPR microorganism activation environment and has the functions of automatically sending the PGPR microorganisms in the external environment into the platform for activation and sending the activated PGPR microorganisms back into the external water environment.
5. The technique for combined phytoremediation of an isapgpr-enriched river or lake according to claim 1, wherein: in S1, the artificial reaction platform can be set as water surface equipment or on-shore equipment according to the water body environment and the standing condition, the water surface artificial reaction platform consists of a foaming buoyancy tank, a reaction tank, an information feedback device and the like, can float along with the fluctuation of the water level, the on-shore equipment is fixed on the shore near the center of a water area, polluted water is pumped into a large-sized bucket filled with an ecological restoration agent through a water pump, and is discharged into the water body at a certain flow rate after circulation, so that a self-circulation process of water flow from the external environment to the inside of the system and then to the external environment is constructed.
6. The technique for combined phytoremediation of an isapgpr-enriched river or lake according to claim 1, wherein: the purification function of the PGPR microorganisms on eutrophic water is mainly divided into three types: aerobic denitrification, phosphorus accumulating bacteria, food chain transfer nitrogen and phosphorus and biological dredging, wherein the aerobic denitrification is aimed at reducing the concentration of ammonia nitrogen in a water body, the phosphorus accumulating bacteria and the food chain transfer nitrogen and phosphorus are aimed at reducing eutrophic substances such as nitrogen and phosphorus in the water body, and the biological dredging can be used for rapidly decomposing and removing organic matters in a water area.
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