CN116410851A - Eddy current sampling device and sampling method - Google Patents
Eddy current sampling device and sampling method Download PDFInfo
- Publication number
- CN116410851A CN116410851A CN202310670856.9A CN202310670856A CN116410851A CN 116410851 A CN116410851 A CN 116410851A CN 202310670856 A CN202310670856 A CN 202310670856A CN 116410851 A CN116410851 A CN 116410851A
- Authority
- CN
- China
- Prior art keywords
- sampling
- liquid
- collecting
- vortex
- collection
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000005070 sampling Methods 0.000 title claims abstract description 268
- 238000000034 method Methods 0.000 title claims abstract description 39
- 239000007788 liquid Substances 0.000 claims abstract description 351
- 244000005700 microbiome Species 0.000 claims abstract description 51
- 238000004140 cleaning Methods 0.000 claims description 91
- 230000008569 process Effects 0.000 claims description 16
- 238000002347 injection Methods 0.000 claims description 7
- 239000007924 injection Substances 0.000 claims description 7
- 230000005611 electricity Effects 0.000 claims description 3
- 230000007246 mechanism Effects 0.000 claims description 3
- 238000005406 washing Methods 0.000 claims 17
- 238000001514 detection method Methods 0.000 abstract description 7
- 230000002572 peristaltic effect Effects 0.000 description 18
- 239000000243 solution Substances 0.000 description 13
- 239000002245 particle Substances 0.000 description 7
- 230000001502 supplementing effect Effects 0.000 description 5
- 244000000010 microbial pathogen Species 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000004907 flux Effects 0.000 description 2
- 239000008187 granular material Substances 0.000 description 2
- 230000005484 gravity Effects 0.000 description 2
- 239000013589 supplement Substances 0.000 description 2
- 208000035473 Communicable disease Diseases 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000000443 aerosol Substances 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000000428 dust Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/10—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by centrifugation ; Cyclones
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/22—Devices for withdrawing samples in the gaseous state
- G01N1/24—Suction devices
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E30/00—Energy generation of nuclear origin
- Y02E30/30—Nuclear fission reactors
Abstract
The invention discloses an eddy current sampling device and a sampling method, which relate to the technical field of microorganism detection, wherein a collecting structure is arranged at the upper part of an eddy current generating structure, an eddy current generating cavity is arranged in the eddy current generating structure, an air inlet is arranged in an air inlet structure, the air inlet is communicated with the eddy current generating cavity, a collecting cavity is arranged in the collecting structure, the collecting cavity is communicated with the eddy current generating cavity, a sampling liquid storage structure is used for storing sampling liquid, the sampling liquid collecting structure is used for collecting the sampling liquid and microorganisms, the sampling liquid storage structure is communicated with the collecting cavity through a sampling liquid storage pipeline, the sampling liquid collecting structure is communicated with the collecting cavity through a sampling liquid collecting pipeline, pump structures are respectively arranged on the sampling liquid storage pipeline and the sampling liquid collecting pipeline, a sensor is arranged in the collecting cavity, the sensor is used for detecting the liquid level of the sampling liquid, and a central controller is respectively electrically connected with the sensor and each pump structure. The invention can automatically, stably and long-time collect the microorganisms in the air at high flow rate.
Description
Technical Field
The invention relates to the technical field of microorganism detection, in particular to an eddy current sampling device and a sampling method.
Background
Microorganisms in human and animal bodies and soil can be dispersed in the air by droplets, dust, or the like, so that the air contains a certain kind and amount of microorganisms. In air there is generally no pathogenic microorganism in theory, but in air around hospitals, veterinary hospitals and livestock and poultry houses, aerosols of pathogenic microorganisms are often suspended, and healthy people or animals often become infected by inhalation. Air contaminated with pathogenic microorganisms can often become a source or medium of contamination, causing the epidemic of infection. Therefore, the detection of airborne microorganisms is of great importance for the prevention and control of infectious diseases and the supervision and protection of environmental hygiene.
The air sampler is an important tool in the air microorganism detection process, and has various types, wherein the impact air sampler collects microorganism particles in the air in sampling liquid by utilizing an air jet mode. In the sampling process, the air pump is started, air enters from the air inlet of the sampler, and after the sampling liquid is added into the collecting area of the sampler, microorganism particles in the air impact into the sampling liquid of the sampler, and the microorganism particles are captured due to the adhesiveness of the liquid.
The prior art has the following disadvantages:
the existing air microorganism detection process mainly depends on manual work, the collection, replacement and other processes of air microorganism samples are completed by manually operating various instruments and equipment, the operation is complex, the time consumption is long, the technical requirement is high, and the requirement of continuously collecting air microorganisms cannot be met.
The current low flow rate (flux) sampler has little consumption of sampling liquid, can collect microorganisms in the air for a long time, but has low flow rate, can not collect microorganisms in the air comprehensively, and the collected sample can not reflect the conditions of the microorganisms in the environment accurately.
The existing high-flow-rate (flux) sampler can impact the liquid level of the sampling liquid violently during high-speed sampling, so that the sampling liquid is lost in a short time and cannot be sampled for a long time. The existing design can not meet the requirements of high flow rate, stability and long-time collection of microorganisms in air.
The existing air microorganism collector, even if being equipped with a liquid supplementing device, can only mechanically supplement liquid in the working process, can not be adjusted in time according to the sampling change condition, can not monitor the volume of the sampling liquid, and has poor equipment controllability.
The sampling liquid of the existing sampling device is arranged at the bottom, so that large particles in air inevitably fall into the sampling liquid to influence the purity of a sample.
Disclosure of Invention
The invention aims to provide an eddy current sampling device and a sampling method, which can automatically, stably and long-time collect microorganisms in air at high flow rate, prevent large particles from falling into a sampling liquid and ensure the purity of a sample.
In order to achieve the above object, the present invention provides the following solutions:
the invention provides a vortex sampling device which comprises an air inlet structure, a vortex generating structure, a collecting structure, a sampling liquid storage structure, a sampling liquid collecting structure and a central controller, wherein the collecting structure is arranged at the upper part of the vortex generating structure, a vortex generating cavity is arranged in the vortex generating structure, an air inlet is arranged in the air inlet structure and communicated with the vortex generating cavity, the collecting cavity is arranged in the collecting structure and communicated with the vortex generating cavity, the sampling liquid storage structure is used for storing sampling liquid, the sampling liquid collecting structure is used for collecting the sampling liquid and microorganisms, the sampling liquid storage structure is communicated with the collecting cavity through a sampling liquid storage pipeline, the sampling liquid collecting structure is communicated with the collecting cavity through a sampling liquid collecting pipeline, pump structures are respectively arranged on the sampling liquid storage pipeline and the sampling liquid collecting pipeline, a sensor is arranged in the collecting cavity and used for detecting the liquid level of the sampling liquid, and the central controller is respectively and electrically connected with the sensor and each pump structure.
Preferably, the device further comprises a cleaning liquid storage structure and a cleaning liquid collection structure, wherein the cleaning liquid storage structure is used for storing cleaning liquid, the cleaning liquid collection structure is used for collecting the cleaning liquid, the cleaning liquid storage structure is communicated with a cleaning liquid injection opening at the lower part of the vortex generating cavity through a cleaning liquid storage pipeline, the cleaning liquid collection structure is communicated with the top of the collection cavity through a cleaning liquid collection pipeline, and the cleaning liquid storage pipeline and the cleaning liquid collection pipeline are respectively provided with a pump structure.
Preferably, the sampling liquid storage structure and the cleaning liquid storage structure are respectively provided with a liquid level meter, and each liquid level meter is electrically connected with the central controller.
Preferably, the device further comprises a solenoid valve, wherein the solenoid valve is electrically connected with the central controller, the solenoid valve is communicated with the top of the collecting cavity through a main pipeline, the solenoid valve is communicated with the sampling liquid storage structure through a sampling liquid storage pipeline, the solenoid valve is communicated with the sampling liquid collection structure through a sampling liquid collection pipeline, the solenoid valve is communicated with the cleaning liquid storage structure through a cleaning liquid storage pipeline, and the solenoid valve is communicated with the cleaning liquid collection structure through a cleaning liquid collection pipeline.
Preferably, the vortex generating structure comprises a vortex generating cylinder, the vortex generating cylinder comprises a straight cylinder and a cone cylinder, the cone cylinder is positioned at the top of the straight cylinder, the upper end of the cone cylinder is connected with the collecting structure, an air inlet channel of the air inlet mechanism is communicated with the straight cylinder, the air inlet channel is tangential to the inner wall of the straight cylinder, and an air outlet channel is arranged in the straight cylinder and is communicated with an opening at the bottom of the straight cylinder.
Preferably, a positive pressure fan is arranged at the air inlet.
Preferably, the system further comprises a battery, wherein the battery is electrically connected with the central controller and each pump structure respectively.
The invention also provides a sampling method of the vortex sampling device, which comprises the following steps:
step one, electrifying a positive pressure fan, and forming vortex in a vortex generating cavity;
step two, introducing cleaning liquid into the vortex generating cavity through the cleaning liquid storage structure, enabling the cleaning liquid to spirally rise along the inner wall of the vortex generating cavity, collecting the cleaning liquid in the collecting cavity, and flowing into the cleaning liquid collecting structure;
step three, after the cleaning is finished, introducing sampling liquid into the collecting cavity through the sampling liquid storage structure until the liquid level of the sampling liquid is separated from the contact point of the sensor, and along with the sampling, when the sensor contact point detects the sampling liquid, controlling the sampling liquid storage structure to introduce the sampling liquid into the collecting cavity until the liquid level of the sampling liquid is separated from the contact point of the sensor by the central controller, wherein the process is operated circularly;
and step four, stopping the introduction of the sampling liquid after the collection time is reached, changing the flow speed of the positive pressure fan, converging the sampling liquid and the liquid drop groups formed by microorganisms into a collection cavity, continuously collecting the liquid drop groups into a sampling liquid collection structure, and ending the single sampling.
The invention also provides a sampling method of the vortex sampling device, which comprises the following steps:
step one, electrifying a positive pressure fan, and forming vortex in a vortex generating cavity;
injecting cleaning liquid into the vortex generating structure through the cleaning liquid injection port to clean microorganisms on the inner walls of the vortex generating cavity and the collecting cavity;
step three, after the cleaning is finished, introducing sampling liquid into the collecting cavity through the sampling liquid storage structure until the liquid level of the sampling liquid is separated from the contact point of the sensor, and along with the sampling, when the sensor contact point detects the sampling liquid, controlling the sampling liquid storage structure to introduce the sampling liquid into the collecting cavity until the liquid level of the sampling liquid is separated from the contact point of the sensor by the central controller, wherein the process is operated circularly;
and step four, stopping the introduction of the sampling liquid after the collection time is reached, changing the flow speed of the positive pressure fan, converging the sampling liquid and the liquid drop groups formed by microorganisms into a collection cavity, continuously collecting the liquid drop groups into a sampling liquid collection structure, and ending the single sampling.
The invention also provides a sampling method of the vortex sampling device, which comprises the following steps:
the method comprises the following steps:
step one, cleaning microorganisms on the inner walls of a vortex generating cavity and a collecting cavity;
step two, after cleaning, installing the vortex generating structure and the collecting structure, electrifying a positive pressure fan, and forming vortex in a vortex generating cavity; introducing sampling liquid into the collecting cavity through the sampling liquid storage structure until the liquid level of the sampling liquid is separated from the contact point of the sensor, and along with sampling, when the sensor contact point detects the sampling liquid, controlling the sampling liquid storage structure to introduce the sampling liquid into the collecting cavity until the liquid level of the sampling liquid is separated from the contact point of the sensor by the central controller, wherein the process is operated circularly;
and thirdly, stopping the introduction of the sampling liquid after the collection time is reached, changing the flow speed of the positive pressure fan, converging the sampling liquid and the liquid drop clusters formed by microorganisms into a collection cavity, continuously collecting the liquid drop clusters into a sampling liquid collection structure, and ending the single sampling.
Compared with the prior art, the invention has the following technical effects:
the invention adopts a wet wall cyclone sampling method, utilizes the vortex generating structure to convert high-speed airflow into vortex, achieves high-flow sampling, and realizes short-time and high-flux collection of microorganisms in air. The pump structure is adopted to control the inflow or outflow of the sampling liquid, so as to realize the purpose of continuously collecting air microorganisms. The collection structure is located the upper portion of vortex generation structure, and big granule falls to the vortex generation structure under the effect of gravity in, avoided big granule to get into in the sampling liquid collection structure along with the sampling liquid, guaranteed the purity degree of sample.
The invention has reliable structure, the sampling process can be automatically adjusted according to the requirements and the actual state, the sampling state can be perceived without manual intervention, the operations of liquid supplementing, collecting and the like can be automatically completed, the cleaning is easy, and meanwhile, the influence of mixed bacteria pollution and the like caused by manual operation is avoided. The invention can automatically, controllably, stably and stably collect microorganisms in the air for a long time at a high flow rate.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are needed in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is an external schematic view of an eddy current sampling apparatus according to the invention (first embodiment);
FIG. 2 is an isometric view of the internal structure of the vortex sampling apparatus according to the present invention (first embodiment);
FIG. 3 is a front view showing the internal structure of the vortex sampling apparatus according to the present invention (first embodiment);
FIG. 4 is a right side view of the internal structure of the vortex sampling apparatus according to the present invention (first embodiment);
FIG. 5 is a left side view of the internal structure of the vortex sampling apparatus according to the present invention (first embodiment);
FIG. 6 is a top view showing the internal structure of the vortex sampling apparatus according to the present invention (first embodiment);
FIG. 7 is a bottom view of the internal structure of the vortex sampling apparatus according to the present invention (first embodiment);
FIG. 8 is a schematic view of a vortex generating structure, a collection structure and a blower of the present invention;
FIG. 9 is a schematic view of the internal structure of the vortex generating structure and the collecting structure of the present invention;
FIG. 10 is a schematic view of an intake passage according to the present invention;
FIG. 11 is a graph of flow rate after simulated fan startup vortex stabilization;
FIG. 12 is a diagram of the liquid level after the simulated fan is started and the vortex is stabilized;
FIG. 13 is an isometric view of an eddy current sampling apparatus according to the invention (second embodiment);
FIG. 14 is a front view of an eddy current sampling apparatus according to the invention (second embodiment);
FIG. 15 is a side view of an eddy current sampling apparatus according to the invention (second embodiment);
FIG. 16 is an exploded view of an eddy current sampling apparatus according to the invention (second embodiment);
wherein: 1-vortex generating structure, 2-collecting structure, 3-vortex generating cavity, 4-collecting cavity, 5-air inlet, 6-sampling liquid storage structure, 7-sampling liquid collecting structure, 8-pump structure, 9-sensor, 10-straight cylinder, 11-cone cylinder, 12-air inlet channel, 13-air outlet channel, 14-bottom plate, 15-opening, 16-cleaning liquid storage structure, 17-cleaning liquid collecting structure, 18-battery, 19-electromagnetic valve, 20-shell, 21-cleaning liquid filling opening.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by a person skilled in the art based on the embodiments of the invention without any inventive effort, are intended to fall within the scope of the invention.
The invention aims to provide an eddy current sampling device and a sampling method, which can automatically, stably and long-time collect microorganisms in air at high flow rate, prevent large particles from falling into a sampling liquid and ensure the purity of a sample.
In order that the above-recited objects, features and advantages of the present invention will become more readily apparent, a more particular description of the invention will be rendered by reference to the appended drawings and appended detailed description.
Example 1
As shown in fig. 1 to 12: the embodiment provides an eddy current sampling device, including casing 20 and the structure that admits air that sets up in casing 20 inside, vortex generation structure 1, collection structure 2, sample liquid storage structure 6, sample liquid collection structure 7 and central controller, the upper portion of vortex generation structure 1 is provided with collection structure 2, the inside of vortex generation structure 1 is vortex generation chamber 3, the structure that admits air is provided with air inlet 5, air inlet 5 is located casing 20, air inlet 5's position is adjustable, air inlet 5 department is provided with positive pressure fan, air inlet 5 and vortex generation chamber 3 intercommunication, collection structure 2's inside is collection chamber 4, collection chamber 4 and vortex generation chamber 3 intercommunication, sample liquid storage structure 6 is used for storing the sample liquid, be provided with the level gauge in the sample liquid storage structure 6, the level gauge all is connected with the central controller electricity, sample liquid collection structure 7 is used for collecting sample liquid and microorganism, sample liquid collection structure 7 is used for single sampling, sample liquid storage structure 6 and collection chamber 4 are through sample liquid storage pipeline intercommunication, sample liquid collection structure 7 and collection chamber 4 are through sample liquid collection pipeline intercommunication, sample liquid storage pipeline and sample liquid storage pipeline are provided with sample liquid collection pipeline 8 on the road respectively, sample liquid storage pipeline and sample liquid storage pipeline are provided with liquid detection pump 8, preferably has sensor 8, sensor 9 is used for detecting each, sensor 9 is used for detecting in the sensor is connected with each sensor 9, for example, sensor 9 is used to detect, and is used for detecting. In this embodiment, the collection chamber 4 is disposed at the top of the vortex generation chamber 3, and large particle impurities are not easily remained in the sampling liquid due to the influence of gravity. The droplets in the collection chamber 4 are subject to their own weight and to the upward force of the vortex, which naturally has a tendency to flow down the walls of the tube, the force of the vortex forcing the droplets to dynamically equilibrate in the collection chamber 4. Compared with a collecting cavity 4 arranged at the bottom conventionally, the force generated by vortex excites the sampling liquid, the sampling liquid is easier to lose under the condition of large flow rate, the sampling liquid in the embodiment is easier to fully contact with air under the same flow rate, and the sampling efficiency is improved.
Specifically, in this embodiment, the vortex generating structure 1 includes a vortex generating cylinder, the vortex generating cylinder includes a straight cylinder 10 and a cone cylinder 11, the cone cylinder 11 is located at the top of the straight cylinder 10, and the upper end of the cone cylinder 11 is connected with the collecting structure 2, an air inlet channel 12 of the air inlet mechanism is communicated with the straight cylinder 10, and the air inlet channel 12 is tangential to the inner wall of the straight cylinder 10, an air outlet channel 13 is provided in the straight cylinder 10, and the air outlet channel 13 is communicated with an opening at the bottom of the straight cylinder 10.
The embodiment also comprises an electromagnetic valve 19, wherein the electromagnetic valve 19 is electrically connected with the central controller, the electromagnetic valve 19 is communicated with the top of the collecting cavity 4 through a main pipeline, the electromagnetic valve 19 is communicated with the sampling liquid storage structure 6 through a sampling liquid storage pipeline, and the electromagnetic valve 19 is communicated with the sampling liquid collecting structure 7 through a sampling liquid collecting pipeline.
The embodiment further comprises a cleaning liquid storage structure 16 and a cleaning liquid collection structure 17, wherein the cleaning liquid storage structure 16 is used for storing cleaning liquid, a liquid level meter is arranged in the cleaning liquid storage structure 16, the liquid level meter is electrically connected with the central controller, the cleaning liquid collection structure 17 is used for collecting the cleaning liquid, the cleaning liquid storage structure 16 is communicated with a cleaning liquid injection port at the lower part of the vortex generating cavity 3 through a cleaning liquid storage pipeline, the cleaning liquid collection structure 17 is communicated with the top of the collecting cavity 4 through a cleaning liquid collection pipeline, and pump structures 8 are respectively arranged on the cleaning liquid storage pipeline and the cleaning liquid collection pipeline.
In this embodiment, the electromagnetic valve 19 communicates with the cleaning liquid storage structure 16 through a cleaning liquid storage pipeline, and the electromagnetic valve 19 communicates with the cleaning liquid collection structure 17 through a cleaning liquid collection pipeline.
The present embodiment further comprises a battery 18, the battery 18 being electrically connected to the central controller and to each pump structure 8, the battery 18 being adapted to supply power to each structure.
The central controller in the embodiment can be internally provided with a program, can set relevant parameters of device sampling, and realizes the automatic detection of air microorganisms by the system.
The embodiment adopts a wet wall cyclone sampling method, and utilizes the vortex generating structure 1 to convert high-speed air flow into vortex, so as to achieve high-flow sampling and realize short-time and high-flux collection of microorganisms in air. And a peristaltic pump is adopted to control the inflow or outflow of the sampling liquid and the cleaning liquid, so that the purpose of continuously collecting air microorganisms is realized. The embodiment can automatically collect microorganisms in the air at a high flow rate and stably collect the microorganisms in the air for a long time. The air microorganisms in the space can be collected in different time periods, manual intervention is not needed in the sampling process, the operations such as cleaning, liquid supplementing and collecting can be automatically completed, and the influence of mixed bacteria pollution and the like caused by manual operation is avoided. By adopting the embodiment, self-cleaning can be automatically completed, the purity among different samples is ensured, automatic collection of air microorganisms can be realized, manpower, material resources and time cost are saved, and the method is convenient and quick.
Example two
The present embodiment provides a sampling method of the vortex sampling device of the first embodiment, including the following steps:
step one, device self-checking after the device is electrified, namely detecting the stock of sampling liquid and cleaning liquid through a liquid level meter; the positive pressure fan is electrified, vortex is formed in the vortex generating cavity 3 along with the air entering, and the collecting cavity 4 is subjected to upward force of the vortex;
step two, a peristaltic pump on a cleaning solution storage pipeline is started, an electromagnetic valve 19 is started, cleaning solution in a cleaning solution storage structure 16 is injected into a vortex generating cavity 3 through a cleaning solution injection port at the lower part of the vortex generating cavity 3, the cleaning solution spirally rises along the inner wall of the vortex generating cavity 3, the cleaning solution sufficiently cleans microorganisms on the inner walls of the vortex generating cavity 3 and a collecting cavity 4, the cleaning solution gradually gathers in the collecting cavity 4, the electromagnetic valve 19 is started to a cleaning solution collecting passage after the cleaning solution is cleaned for a required time, and the peristaltic pump of the cleaning solution collecting pipeline is extracted from the collecting cavity 4 into a cleaning solution collecting structure 17;
step three, cleaning liquid in the vortex generating cavity 3 and the collecting cavity 4 is collected cleanly, after the liquid beads on the inner walls of the vortex generating cavity 3 and the collecting cavity 4 disappear, the electromagnetic valve 19 is controlled to open a sampling liquid storage passage, a peristaltic pump on a sampling liquid storage pipeline injects sampling liquid in the sampling liquid storage structure 6 into the collecting cavity 4 until the liquid level of the sampling liquid breaks away from a contact point of a sensor 9 (hovering below the sensor 9), a central controller detects signals of the sensor 9, a peristaltic pump on the sampling liquid storage pipeline stops working, air continuously impacts the liquid level at the bottom to collect microorganisms in the air along with sampling, part of the sampling liquid is taken away, the volume of a liquid drop group becomes smaller, the air pressure in the collecting cavity 4 is larger than the weight of the liquid drop group, the liquid level rises to submerge the contact point of the sensor 9, and when the contact point of the sensor 9 detects the sampling liquid, the central controller controls the peristaltic pump on the sampling liquid storage pipeline to introduce the sampling liquid into the collecting cavity 4, and carry out liquid supplementing until the liquid level of the sampling liquid breaks away from the contact point of the sensor 9, and the process is circularly operated, so that a long-time sampling function is realized;
and step four, after the collection time is reached, the peristaltic pump on the sampling liquid storage pipeline stops injecting the sampling liquid, the flow speed of the positive pressure fan is changed, the force of vortex upwards pushing the collection cavity 4 is increased, the liquid drop group moves to the top of the collection cavity 4 from bottom to top, the electromagnetic valve 19 opens a sampling liquid collection tank passage, the peristaltic pump on the sampling liquid collection pipe works, the liquid drop group is collected into the sampling liquid collection structure 7, and the single sampling is finished.
Example III
As shown in fig. 8 to 16: the difference between this embodiment and the first embodiment is that: the cleaning liquid storage structure 16 and the cleaning liquid collection structure 17 are not provided in this embodiment. The present embodiment employs manual cleaning.
In this embodiment, the vortex generating structure 1 includes a vortex generating cylinder and a bottom plate 14, the bottom plate 14 is located at the bottom of the vortex generating cylinder, and the bottom plate 14 is detachably connected to the vortex generating cylinder. The air outlet channel 13 in the straight cylinder 10 is communicated with the opening 15 on the bottom plate 14, the opening 15 is communicated with the air outlet on the shell 20, and the position of the air outlet is adjustable.
Example IV
The embodiment provides a sampling method of the eddy current sampling apparatus of the third embodiment, which includes the following steps:
step one, device self-checking after the device is electrified, namely detecting the stock of the sampling liquid through a liquid level meter; the positive pressure fan is electrified, vortex is formed in the vortex generating cavity 3 along with the air entering, and the collecting cavity 4 is subjected to upward force of the vortex;
step two, manually injecting cleaning liquid into the vortex generating structure 1 through the cleaning liquid injection port 21, and cleaning microorganisms on the inner walls of the vortex generating cavity 3 and the collecting cavity 4 by the cleaning liquid under the action of the positive pressure fan;
step three, after the cleaning is finished, the electromagnetic valve 19 is controlled to open a sampling liquid storage passage, a peristaltic pump on a sampling liquid storage pipeline injects sampling liquid of the sampling liquid storage structure 6 into the collecting cavity 4 until the liquid level of the sampling liquid breaks away from a contact point of the sensor 9 (hovering below the sensor 9), the central controller detects a signal of the sensor 9, the peristaltic pump on the sampling liquid storage pipeline stops working, air continuously impacts the liquid level at the bottom to collect microorganisms in the air along with the sampling, meanwhile, partial sampling liquid is taken away, the volume of a liquid drop group becomes smaller, the air pressure in the collecting cavity 4 is larger than the weight of the liquid drop group, the liquid level rises to submerge the contact point of the sensor 9, when the contact point of the sensor 9 detects the sampling liquid, the central controller controls the peristaltic pump on the sampling liquid storage pipeline, and introduces the sampling liquid into the collecting cavity 4 to supplement the liquid until the liquid level of the sampling liquid breaks away from the contact point of the sensor 9, and the process is circularly operated, so that a long-time sampling function is realized;
and step four, after the collection time is reached, the peristaltic pump on the sampling liquid storage pipeline stops injecting the sampling liquid, the flow speed of the positive pressure fan is changed, the force of vortex upwards pushing the collection cavity 4 is increased, the liquid drop group moves to the top of the collection cavity 4 from bottom to top, the electromagnetic valve 19 opens a sampling liquid collection tank passage, the peristaltic pump on the sampling liquid collection pipe works, the liquid drop group is collected into the sampling liquid collection structure 7, and the single sampling is finished.
Example five
The difference between this embodiment and the fourth embodiment is that: the vortex generating structure 1 and the collecting structure 2 are disassembled, and microorganisms on the inner walls of the vortex generating cavity 3 and the collecting cavity 4 are manually cleaned by adopting cleaning liquid.
Specifically, the present embodiment provides a sampling method of the vortex sampling device of the third embodiment, including the following steps:
step one, cleaning microorganisms on the inner walls of the vortex generating cavity 3 and the collecting cavity 4 by adopting cleaning liquid;
step two, after cleaning, installing an eddy current generating structure 1 and a collecting structure 2, and performing self-inspection on the device after the device is electrified, namely detecting the stock of the sampling liquid through a liquid level meter; the positive pressure fan is electrified, vortex is formed in the vortex generating cavity 3 along with the air entering, and the collecting cavity 4 is subjected to upward force of the vortex; the electromagnetic valve 19 is controlled to open a sampling liquid storage passage, a peristaltic pump on a sampling liquid storage pipeline injects sampling liquid of the sampling liquid storage structure 6 into the collecting cavity 4 until the liquid level of the sampling liquid breaks away from a contact point of the sensor 9 (hovering below the sensor 9), the central controller detects a signal of the sensor 9, the peristaltic pump on the sampling liquid storage pipeline stops working, air continuously impacts the bottom liquid level to collect microorganisms in the air along with sampling, part of the sampling liquid is taken away, the volume of a liquid drop group becomes smaller, the air pressure in the collecting cavity 4 is larger than the weight of the liquid drop group, the liquid level rises to submerge the contact point of the sensor 9, when the contact point of the sensor 9 detects the sampling liquid, the central controller controls the peristaltic pump on the sampling liquid storage pipeline, and the sampling liquid is introduced into the collecting cavity 4 for supplementing until the liquid level of the sampling liquid breaks away from the contact point of the sensor 9, and the process is circularly operated, so that a long-time sampling function is realized;
and thirdly, after the collection time is reached, stopping the injection of the sampling liquid by a peristaltic pump on a sampling liquid storage pipeline, changing the flow speed of the positive pressure fan, increasing the force of the vortex on the collection cavity 4, moving the liquid drop mass from bottom to top to the top of the collection cavity 4, opening a sampling liquid collection tank passage by an electromagnetic valve 19, operating the peristaltic pump on the sampling liquid collection pipe, collecting the liquid drop mass into a sampling liquid collection structure 7, and ending single sampling.
The principles and embodiments of the present invention have been described in this specification with reference to specific examples, the description of which is only for the purpose of aiding in understanding the method of the present invention and its core ideas; also, it is within the scope of the present invention to be modified by those of ordinary skill in the art in light of the present teachings. In view of the foregoing, this description should not be construed as limiting the invention.
Claims (10)
1. An eddy current sampling device, characterized in that: including inlet structure, vortex generation structure, collection structure, sampling liquid storage structure, sampling liquid collection structure and central controller, vortex generation structure's upper portion is provided with collection structure, vortex generation structure's inside is the vortex and takes place the chamber, inlet structure is provided with the air inlet, the air inlet with the chamber intercommunication takes place for the vortex, collection structure's inside is the collection chamber, the collection chamber with the chamber intercommunication takes place for the vortex, sampling liquid storage structure is used for storing the sampling liquid, sampling liquid collection structure is used for collecting sampling liquid and microorganism, sampling liquid storage structure with the collection chamber passes through sampling liquid storage pipeline intercommunication, sampling liquid collection structure with the collection chamber passes through sampling liquid collection pipeline intercommunication, sampling liquid storage pipeline with be provided with pump structure on the sampling liquid collection pipeline respectively, be provided with the sensor in the collection chamber, the sensor is used for detecting the liquid level of sampling liquid, central controller respectively with sensor and each pump structure electricity are connected.
2. The vortex sampling device of claim 1, wherein: still include washing liquid storage structure and washing liquid collection structure, washing liquid storage structure is used for storing the washing liquid, washing liquid collection structure is used for collecting the washing liquid, washing liquid storage structure with the washing liquid filling opening of the lower part of vortex generation chamber passes through the washing liquid and stores the pipeline intercommunication, washing liquid collection structure with the top of collecting the chamber passes through the washing liquid and collects the pipeline intercommunication, washing liquid storage pipeline with be provided with pump structure on the washing liquid collecting pipe way respectively.
3. The vortex sampling device of claim 2, wherein: the liquid level meters are arranged in the sampling liquid storage structure and the cleaning liquid storage structure, and are electrically connected with the central controller.
4. The vortex sampling device of claim 2, wherein: still include the solenoid valve, the solenoid valve with the central controller electricity is connected, the solenoid valve with the top of collecting the chamber is through total pipeline intercommunication, the solenoid valve with the sampling liquid stores the structure and passes through the sampling liquid stores the pipeline intercommunication, the solenoid valve with the sampling liquid collects the structure and passes through the sampling liquid collects the pipeline intercommunication, the solenoid valve with the washing liquid stores the structure and passes through the washing liquid stores the pipeline intercommunication, the solenoid valve with the washing liquid collects the structure and passes through the washing liquid collects the pipeline intercommunication.
5. The vortex sampling device of claim 1, wherein: the vortex generating structure comprises a vortex generating cylinder, the vortex generating cylinder comprises a straight cylinder and a conical cylinder, the conical cylinder is located at the top of the straight cylinder, the upper end of the conical cylinder is connected with the collecting structure, an air inlet channel of the air inlet mechanism is communicated with the straight cylinder, the air inlet channel is tangential to the inner wall of the straight cylinder, an air outlet channel is arranged in the straight cylinder, and the air outlet channel is communicated with an opening at the bottom of the straight cylinder.
6. The vortex sampling device of claim 1, wherein: and a positive pressure fan is arranged at the air inlet.
7. The vortex sampling device of claim 1, wherein: the system also comprises a battery which is respectively and electrically connected with the central controller and each pump structure.
8. A sampling method of an eddy current sampling apparatus as claimed in any one of claims 1 to 7, wherein: the method comprises the following steps:
step one, electrifying a positive pressure fan, and forming vortex in a vortex generating cavity;
step two, introducing cleaning liquid into the vortex generating cavity through the cleaning liquid storage structure, enabling the cleaning liquid to spirally rise along the inner wall of the vortex generating cavity, collecting the cleaning liquid in the collecting cavity, and flowing into the cleaning liquid collecting structure;
step three, after the cleaning is finished, introducing sampling liquid into the collecting cavity through the sampling liquid storage structure until the liquid level of the sampling liquid is separated from the contact point of the sensor, and along with the sampling, when the sensor contact point detects the sampling liquid, controlling the sampling liquid storage structure to introduce the sampling liquid into the collecting cavity until the liquid level of the sampling liquid is separated from the contact point of the sensor by the central controller, wherein the process is operated circularly;
and step four, stopping the introduction of the sampling liquid after the collection time is reached, changing the flow speed of the positive pressure fan, converging the sampling liquid and the liquid drop groups formed by microorganisms into a collection cavity, continuously collecting the liquid drop groups into a sampling liquid collection structure, and ending the single sampling.
9. A sampling method of an eddy current sampling apparatus as claimed in any one of claims 1, 5 to 7, wherein: the method comprises the following steps:
step one, electrifying a positive pressure fan, and forming vortex in a vortex generating cavity;
injecting cleaning liquid into the vortex generating structure through the cleaning liquid injection port to clean microorganisms on the inner walls of the vortex generating cavity and the collecting cavity;
step three, after the cleaning is finished, introducing sampling liquid into the collecting cavity through the sampling liquid storage structure until the liquid level of the sampling liquid is separated from the contact point of the sensor, and along with the sampling, when the sensor contact point detects the sampling liquid, controlling the sampling liquid storage structure to introduce the sampling liquid into the collecting cavity until the liquid level of the sampling liquid is separated from the contact point of the sensor by the central controller, wherein the process is operated circularly;
and step four, stopping the introduction of the sampling liquid after the collection time is reached, changing the flow speed of the positive pressure fan, converging the sampling liquid and the liquid drop groups formed by microorganisms into a collection cavity, continuously collecting the liquid drop groups into a sampling liquid collection structure, and ending the single sampling.
10. A sampling method of an eddy current sampling apparatus as claimed in any one of claims 1, 5 to 7, wherein: the method comprises the following steps:
step one, cleaning microorganisms on the inner walls of a vortex generating cavity and a collecting cavity;
step two, after cleaning, installing the vortex generating structure and the collecting structure, electrifying a positive pressure fan, and forming vortex in a vortex generating cavity; introducing sampling liquid into the collecting cavity through the sampling liquid storage structure until the liquid level of the sampling liquid is separated from the contact point of the sensor, and along with sampling, when the sensor contact point detects the sampling liquid, controlling the sampling liquid storage structure to introduce the sampling liquid into the collecting cavity until the liquid level of the sampling liquid is separated from the contact point of the sensor by the central controller, wherein the process is operated circularly;
and thirdly, stopping the introduction of the sampling liquid after the collection time is reached, changing the flow speed of the positive pressure fan, converging the sampling liquid and the liquid drop clusters formed by microorganisms into a collection cavity, continuously collecting the liquid drop clusters into a sampling liquid collection structure, and ending the single sampling.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310670856.9A CN116410851B (en) | 2023-06-08 | 2023-06-08 | Eddy current sampling device and sampling method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310670856.9A CN116410851B (en) | 2023-06-08 | 2023-06-08 | Eddy current sampling device and sampling method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN116410851A true CN116410851A (en) | 2023-07-11 |
| CN116410851B CN116410851B (en) | 2023-09-08 |
Family
ID=87056353
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310670856.9A Active CN116410851B (en) | 2023-06-08 | 2023-06-08 | Eddy current sampling device and sampling method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN116410851B (en) |
Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030115975A1 (en) * | 1997-12-12 | 2003-06-26 | Research Intertional, Inc. | Air sampler |
| US20070068223A1 (en) * | 2003-10-17 | 2007-03-29 | Chen Teh-Hsun B | Air-sampling device and method of use |
| JP2012052866A (en) * | 2010-08-31 | 2012-03-15 | Osaka Gas Co Ltd | Virus collection device and virus inspection system |
| US8307724B1 (en) * | 2008-10-21 | 2012-11-13 | Nomadics, Inc. | Two mode vapor/particulate sampling device |
| CN104280273A (en) * | 2013-07-02 | 2015-01-14 | 香港城市大学 | High flow capacity multi-function aerosol-to-hydrosol air sampler and sampling method |
| CN111417853A (en) * | 2018-04-06 | 2020-07-14 | 松下知识产权经营株式会社 | Pathogen detection device and pathogen detection method |
| CN211553460U (en) * | 2020-02-13 | 2020-09-22 | 北京慧荣和科技有限公司 | Wet-wall cyclone type biological aerosol sampler |
| CN112033762A (en) * | 2020-01-23 | 2020-12-04 | 北京鼎蓝科技有限公司 | Portable air sampler and sampling method thereof |
| CN213803779U (en) * | 2020-11-06 | 2021-07-27 | 江西省儿童医院 | Air sample collector for physical and chemical inspection |
| CN113789254A (en) * | 2021-08-09 | 2021-12-14 | 哈尔滨工业大学（深圳） | Air acquisition module and analysis and detection device |
| CN113916618A (en) * | 2021-09-29 | 2022-01-11 | 中科合肥技术创新工程院 | Online virus monitoring and early warning device |
-
2023
- 2023-06-08 CN CN202310670856.9A patent/CN116410851B/en active Active
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030115975A1 (en) * | 1997-12-12 | 2003-06-26 | Research Intertional, Inc. | Air sampler |
| US20070068223A1 (en) * | 2003-10-17 | 2007-03-29 | Chen Teh-Hsun B | Air-sampling device and method of use |
| US8307724B1 (en) * | 2008-10-21 | 2012-11-13 | Nomadics, Inc. | Two mode vapor/particulate sampling device |
| JP2012052866A (en) * | 2010-08-31 | 2012-03-15 | Osaka Gas Co Ltd | Virus collection device and virus inspection system |
| CN104280273A (en) * | 2013-07-02 | 2015-01-14 | 香港城市大学 | High flow capacity multi-function aerosol-to-hydrosol air sampler and sampling method |
| CN111417853A (en) * | 2018-04-06 | 2020-07-14 | 松下知识产权经营株式会社 | Pathogen detection device and pathogen detection method |
| CN112033762A (en) * | 2020-01-23 | 2020-12-04 | 北京鼎蓝科技有限公司 | Portable air sampler and sampling method thereof |
| CN211553460U (en) * | 2020-02-13 | 2020-09-22 | 北京慧荣和科技有限公司 | Wet-wall cyclone type biological aerosol sampler |
| CN213803779U (en) * | 2020-11-06 | 2021-07-27 | 江西省儿童医院 | Air sample collector for physical and chemical inspection |
| CN113789254A (en) * | 2021-08-09 | 2021-12-14 | 哈尔滨工业大学（深圳） | Air acquisition module and analysis and detection device |
| CN113916618A (en) * | 2021-09-29 | 2022-01-11 | 中科合肥技术创新工程院 | Online virus monitoring and early warning device |
Also Published As
| Publication number | Publication date |
|---|---|
| CN116410851B (en) | 2023-09-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN211553460U (en) | Wet-wall cyclone type biological aerosol sampler | |
| KR101510254B1 (en) | System and method for collecting and detecting airborne particles | |
| CN102634449B (en) | Viral aerosol collecting and enriching instrument | |
| CN105092454A (en) | Counter and flow-type cell analyzer | |
| CN106085830B (en) | Pathogenic microorganism collection device in air | |
| CN103381422A (en) | Novel kitchen waste treatment device | |
| CN1654944A (en) | Radioactive aerosol granularity separating and sampling unit | |
| CN116410851B (en) | Eddy current sampling device and sampling method | |
| CN204786839U (en) | PM2. 5 intelligence clarifier based on singlechip | |
| CN114279775A (en) | Sampling device for monitoring bioaerosol | |
| CN108120623A (en) | A kind of diesel engine particulate object grading sampling device and its control method | |
| CN202744553U (en) | Mycoplasma hyopneumoniae aerosol sampler | |
| CN103017983B (en) | Capacitor sensing type bag-type dust collector leak detector | |
| CN209153420U (en) | Hand-held cleaners | |
| CN207102161U (en) | Intelligent PM2.5 detects filtration purifier | |
| CN203117079U (en) | Fabric shielding efficiency testing device | |
| KR101273421B1 (en) | Wet cyclone to concentrate and collect biological particles in air | |
| CN107559996A (en) | A kind of air purifier | |
| CN214793951U (en) | Turbidity appearance preliminary treatment bubble removing device | |
| CN208621404U (en) | A kind of sample introduction automatic filtering unit | |
| CN109060620B (en) | Spray drying type standard dust aerosol generation and verification device | |
| CN207779772U (en) | A kind of bioaerosol sampler based on empty cyclonic principle | |
| CN205958414U (en) | Inhalable particle aerosol generating device | |
| KR200479874Y1 (en) | cyclone dust collector of flow control type | |
| CN206974471U (en) | A kind of dual field air-locked type electric cleaner test device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |