CN116406615A - Method for breeding rice high-temperature-resistant light (temperature) -sensitive nuclear male sterile line - Google Patents
Method for breeding rice high-temperature-resistant light (temperature) -sensitive nuclear male sterile line Download PDFInfo
- Publication number
- CN116406615A CN116406615A CN202310371700.0A CN202310371700A CN116406615A CN 116406615 A CN116406615 A CN 116406615A CN 202310371700 A CN202310371700 A CN 202310371700A CN 116406615 A CN116406615 A CN 116406615A
- Authority
- CN
- China
- Prior art keywords
- temperature
- plant
- generation
- sterile
- rice
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 235000007164 Oryza sativa Nutrition 0.000 title claims abstract description 50
- 235000009566 rice Nutrition 0.000 title claims abstract description 50
- 238000000034 method Methods 0.000 title claims abstract description 28
- 238000009395 breeding Methods 0.000 title claims abstract description 24
- 230000001488 breeding effect Effects 0.000 title claims abstract description 24
- 240000007594 Oryza sativa Species 0.000 title 1
- 241000196324 Embryophyta Species 0.000 claims abstract description 113
- 241000209094 Oryza Species 0.000 claims abstract description 49
- 238000009401 outcrossing Methods 0.000 claims abstract description 20
- 238000004519 manufacturing process Methods 0.000 claims abstract description 15
- 230000013011 mating Effects 0.000 claims abstract description 3
- 230000035800 maturation Effects 0.000 claims abstract description 3
- 238000009396 hybridization Methods 0.000 claims description 16
- 238000012216 screening Methods 0.000 claims description 11
- 230000001172 regenerating effect Effects 0.000 claims description 7
- 238000012360 testing method Methods 0.000 claims description 7
- 238000003306 harvesting Methods 0.000 claims description 6
- 238000012214 genetic breeding Methods 0.000 abstract description 2
- 235000013339 cereals Nutrition 0.000 description 8
- 230000000694 effects Effects 0.000 description 5
- 238000011161 development Methods 0.000 description 4
- 230000018109 developmental process Effects 0.000 description 4
- 230000035558 fertility Effects 0.000 description 4
- 238000011084 recovery Methods 0.000 description 3
- 210000001015 abdomen Anatomy 0.000 description 2
- 230000002146 bilateral effect Effects 0.000 description 2
- 230000010154 cross-pollination Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 2
- 102220470647 Amidophosphoribosyltransferase_Y58S_mutation Human genes 0.000 description 1
- 241000234276 Curculigo Species 0.000 description 1
- 229920002558 Curdlan Polymers 0.000 description 1
- 239000001879 Curdlan Substances 0.000 description 1
- 244000184734 Pyrus japonica Species 0.000 description 1
- 230000009418 agronomic effect Effects 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 229940078035 curdlan Drugs 0.000 description 1
- 235000019316 curdlan Nutrition 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 210000005069 ears Anatomy 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 238000009405 line breeding Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 102200017867 rs121434558 Human genes 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
- 230000003313 weakening effect Effects 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
- A01H1/022—Genic fertility modification, e.g. apomixis
- A01H1/023—Male sterility
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/12—Processes for modifying agronomic input traits, e.g. crop yield
- A01H1/122—Processes for modifying agronomic input traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a method for breeding a rice high-temperature-resistant light (temperature) -sensitive nuclear male sterile line, which specifically comprises the following steps: s1, adopting an artificial glume-cutting, emasculation and hybrid mating method, hybridizing by taking light (temperature) -sensitive nuclear male sterile line Xianshas as a female parent and martial arts B as a male parent to obtain an F1 generation, planting the F1 generation, mixedly collecting the F1 generation after maturation, planting an F2 generation group, and selecting a fertile single plant with high fruiting rate, short plant height and excellent rice quality in the F2 generation; s2, planting the F3 single plant, and performing high-temperature stress treatment on the F3 single plant, and continuously selecting a fertile single plant with high fruiting rate, short plant height and good rice quality; the invention relates to the technical field of plant genetic breeding. The sterile line bred by the method has strong high temperature resistance, good outcrossing property and excellent rice quality, and greatly improves the seed production yield of the sterile line at high temperature.
Description
Technical Field
The invention relates to the technical field of plant genetic breeding, in particular to a method for breeding a rice high-temperature-resistant light (temperature) -sensitive nuclear male sterile line.
Background
In 1973, the indica type three-line hybrid rice is matched in China, and in 1976, the three-line hybrid rice is promoted in a large area, so that great contribution is made to solving the grain safety problem in China. In 1973, mr. Mingshun in Hubei curdlan has found a natural rice abortive strain "agricultural reclamation 58S", and has been studied in many aspects, and after careful cultivation by breeders, a batch of excellent light (temperature) sensitive sterile lines of indica type and japonica type are bred, and large-area popularization and application are obtained, so that a new approach, namely a two-line method, is opened up for the development of hybrid rice in China. The development of hybrid rice from three-line method to two-line method is a great invention in the history of rice in China and is rapidly popularized. The two-line hybrid rice has unique advantages, mainly: the first system is dual-purpose, simplifies the breeding and seed production process, and reduces the production cost of seeds; the diversity of cytoplasm is enriched; is not restricted by the recovery relation, is free to be matched and the like. The new variety cultivated by the two-line method plays an increasingly larger role in increasing grain yield and increasing income of farmers. At present, the most widely utilized light (temperature) sensitive nuclear male sterile lines in production mainly comprise "Guangzhan 63S", "Peubai 64S", "Y58S", "P88S", "HD9802S", "C815S" and the like.
However, in recent years, due to the occurrence of extremely high and low temperature weather, great losses are incurred in the seed production of the two-line hybrid rice, and the better development of the two-line hybrid rice is hindered. The extremely high temperature weather causes the weak activity of the two-line sterile line heads, the outcrossing property is poor, and the seed production yield is low; the two sterile lines are converted into fertility by extremely low temperature weather, so that qualified hybrid seeds cannot be produced, and the quality of the hybrid seeds is seriously affected. Therefore, the breeding of the light (temperature) sensitive male sterile line is a key factor of hybrid vigor utilization of hybrid rice and is also the core content of two-line hybrid rice research.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a method for breeding a rice high-temperature-resistant light (temperature) -sensitive nuclear male sterile line, which is used for improving agronomic characters such as weakening of stigma activity, poor outcrossing and the like of a two-line sterile line at high temperature and improving seed production yield.
In order to achieve the above purpose, the invention is realized by the following technical scheme: a method for breeding a rice high-temperature-resistant light (temperature) -sensitive nuclear male sterile line specifically comprises the following steps:
s1, adopting an artificial glume-cutting, emasculation and hybrid mating method, hybridizing by taking light (temperature) -sensitive nuclear male sterile line Xianshas as a female parent and martial arts B as a male parent to obtain an F1 generation, planting the F1 generation, mixedly collecting the F1 generation after maturation, planting an F2 generation group, and selecting a fertile single plant with high fruiting rate, short plant height and excellent rice quality in the F2 generation;
s2, planting the F3 single plant, and performing high-temperature stress treatment on the F3 single plant, and continuously selecting a fertile single plant with high fruiting rate, short plant height and good rice quality;
s3, selecting an F5 generation, selecting sterile single plants from the F5 generation, screening target characters of the sterile single plants, screening excellent single plants meeting the target characters, cutting stumps, regenerating, bagging and selfing, and harvesting regenerated sub-generation seeds;
s4, regenerating plant lines of plant first-generation single plants, numbering each plant in a tillering stage, dividing the plant lines into two plants, carrying out high-temperature stress treatment on one plant, bagging the plant lines for propagation, selecting excellent sterile single plants meeting target characters under the high-temperature treatment, particularly screening the character single plants with high exposed stigmas, and carrying out restorer hybridization test and allocation on the screened single plants;
s5, counting, hybridizing, measuring and preparing single plant setting percentage, selecting sterile single plants with high outcrossing setting percentage, and harvesting corresponding propagation single plant seeds;
s6, screening out the high temperature resistant rice light (temperature) sensitive nuclear male sterile line meeting the two-line sterile line standard according to the methods of the steps S4 and S5.
Preferably, in the step S1, the F1 is obtained by hybridization with the light (temperature) sensitive nuclear male sterile line as a female parent and excellent conventional rice as a male parent.
Preferably, in the step S3, the offspring of the sterile plant are subjected to simple high Wen Penggao temperature stress, the restorer line is used for hybridization, the outcrossing seed setting rate is counted, and the high-temperature resistant light (temperature) sensitive nuclear male sterile line which accords with the breeding target is selected.
Preferably, the fertile single plant is bred from F2, F3-F4 breeds the fertile single plant by simple high Wen Penggao temperature stress, and the selected single plant has good leaf shape, excellent rice quality, high outcrossing and maturing and high temperature resistance at high temperature.
Preferably, F5 starts to select sterile plants, is always subjected to simple high Wen Penggao temperature stress, is hybridized with sterile plants by using an external restoring line, the outcrossing fruiting rate after hybridization is counted, the selected sterile single plants are high-temperature resistant at high temperature, the outcrossing fruiting rate is utilized to verify the high-temperature resistance, the selected sterile plants are high in high-temperature resistance and good in outcrossing performance, the sterile lines meet high-temperature climates, and the seed production yield is high.
Advantageous effects
The invention provides a method for breeding a rice high-temperature-resistant light (temperature) -sensitive nuclear male sterile line. Compared with the prior art, the method has the following beneficial effects:
the method for breeding the rice high-temperature-resistant light (temperature) -sensitive nuclear male sterile line comprises the steps of continuously subjecting the rice to high-temperature stress treatment from low generation to high generation, carrying out restorer line hybridization test and matching on each generation of sterile line breeding, and screening sterile line single plants with high outcrossing fruiting rate at high temperature; the sterile single plant is bred under high temperature of the high generation, and the sterile line plant is high and low and has good lodging resistance;
the sterile single plant with high outcrossing setting rate is selected under the high temperature stress of the sterile line of the high generation, the sterile line has high stigma exposure, good flowering habit and high outcrossing setting rate, so the seed production yield of the sterile line is high under the high temperature, the influence of the high temperature on the seed production is small, and the glume closing performance of the sterile line is good;
the selected sterile line is high-temperature resistant, good in heat resistance and strong in high-temperature resistance of the matched hybridization combination under continuous high-temperature stress from low generation to high generation.
In conclusion, the method disclosed by the invention is simple and reliable, is easy to popularize, has a great promotion effect on the steady development of high-grade high-quality rice and high-quality two-line hybrid rice in China, can reduce the loss of rice in extremely severe weather, ensures the income increase of peasants, increases the grain yield, and ensures the national grain safety.
Drawings
FIG. 1 is a TY12472S breeding flow chart of the invention;
FIG. 2 is a simplified high temperature processing shed facility of the present invention;
FIG. 3 is a schematic representation of the artificial hybrid heading of TY12472S and Guangzhan 63S under the high temperature treatment of the present invention;
FIG. 4 is a schematic representation of rice grains of the artificial cross pollination of the sterile line of the present invention;
fig. 5 shows the field growth of the new combined two-best 4722 of the present invention.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings are merely for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present invention.
Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include one or more of the described features. In the description of the present invention, the meaning of "a plurality" is two or more, unless explicitly defined otherwise. Furthermore, the terms "mounted," "connected," "coupled," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present invention will be understood in specific cases by those of ordinary skill in the art.
Example 1
The breeding process of TY 12472S:
parent source for breeding:
1. source of photop (temperature) sensitive nuclear male sterile line xiansha 7S: the stock seed selection of the Hubei province curculigo city and the sand lake is carried out in 2010 through the technical identification of the Hubei province agricultural hall organization.
2. Source of martial B: 93-11/IR58025// IR58025 (from the national emphasis laboratory breeding materials of hybrid rice, university of Wuhan, and approved by the Hubei province Committee for variety approval).
The breeding flow is shown in figure 1. After the sand-7S is taken as female parent in the spring Hainan in 2008, the F1 generation seeds are obtained by hybridization with the Wuxian B as male parent, F1 generation seeds are planted in the Wuhan in 2008, the seed is normal, the mature period is early, the rice quality is excellent, all seeds F2 are harvested in a mixed mode, more than 3000 strains of F2 generation groups are planted in the Hainan in 2009, the F2 generation groups are separated, the ratio of the normal single strain to the sterile line single strain is 3:1, the seed setting rate is high, the strain height is short, the tillering power is strong, 100 fertile single strains with excellent rice quality are selected, 100 single strains with F3 generation in summer are planted in the Wuhan in 2009, the simple temperature increasing shed as set up in FIG. 2 is utilized for carrying out high temperature stress treatment on the single strains, the 100 single strains are also separated seriously, the seed setting rate is high, the plant height is selected, the tillering power is strong, the rice quality is excellent, 60 fertile single strains with good plant leaf shape are planted in the mode in the year 2010, the 60F 4 fertile single strains are subjected to high temperature stress treatment by using the setting of FIG 2, selecting 50 fertile single plants with high fruiting rate, short plant height, strong tillering capability, excellent rice quality and good plant leaf shape, planting 50 Wuhan F5 single plants in 2010, carrying out high-temperature stress treatment on the 50 fertile single plants, finding that sterile single plants appear in 14 of the 50 single plants, regenerating the sterile single plants in the rest 36 single plants, carrying out trait selection on the sterile single plants in the 14 single plants, selecting the sterile single plants with high stigma exposure rate, strong tillering, short plant height, good plant leaf shape, small milky white anther, carrying out single planting, carrying out pollen microscopic examination on the sterile single plants in the first year, finding that the sterile single plants are pollen-free sterile single plants, regenerating the selected 11 sterile single plants in the first year, bagging selfing, regenerating seed seeds in the bottom of 10 months, harvesting 11 first-generation single plants in the spring of 2011, cultivating the first-generation single plants in the south of spring, the single plants of each plant line in the tillering stage are marked with numbers, each single plant is divided into two plants, one plant is subjected to high-temperature stress treatment, one plant is subjected to bagging for propagation, 20 sterile single plants which meet the target property are selected for the single plants under the high-temperature treatment, the property of exposing the stigma is particularly paid attention to, meanwhile, male parent restorer line hybridization is carried out on the selected single plants, 20 combinations are hybridized, the maturing stage is counted, the maturing condition of the hybridization of the 20 sterile single plants is carried out, 5 seed setting rates in the 20 combinations reach more than 80%, the rest 15 seed setting rates are lower than 80%, 9 of the sterile single plants are lower than 10%,4 of the sterile single plants are 20% -30%,2 of the seed setting rates are 35% -40%, the maturing rate of the 5 sterile single plants reaches 80%, the corresponding 5 selected propagation single plant seeds are harvested, the method comprises the steps of carrying out high temperature stress treatment on 5 second-generation sterile strains of the Wuhan plantlets in summer in 2011, enabling the 5 strains to be relatively regular in growth vigor, enabling the strains to be different in height, selecting single strains with exposed stigmas and high in male parent recovery line hybridization, measuring and preparing the sterile single strains, enabling the fruiting rate of the sterile single strains to reach more than 70%, selecting 10 single strains from the 5 strains, cutting stumps for regeneration, bagging for selfing, harvesting three-generation sterile single plant seeds, repeatedly selecting to fifth generations, enabling each generation to be subjected to high temperature stress treatment, enabling the sterile lines to be subjected to male parent recovery line fruiting rate verification and investigation, enabling the strain with the number of 12-4-72 to be selected from Hainan in 2013, enabling the strain to be regular and consistent, enabling the strain to be short, enabling the stigma external yield to be high, enabling the sterile line to be bred, and enabling the sterile line to be temporarily named as 12472S. The Wuhan in 2013 is subjected to multi-male parent small-area combined trial production, and in 2013, rare high-temperature weather happens to be encountered, 12472S shows good flowering habit, high fruiting rate and high seed production yield, the mu yield reaches 250 kg, and other sterile lines such as the mu yield of 63S is lower than 50 kg. The 12472S is subjected to artificial air temperature box fertility identification screening at the university of Wuhan in the same year, and low-temperature fertility screening is continued in 2014 to breed sterile single plants meeting the light (temperature) sensitive nuclear male sterile line standard. The sterile line is designated TY12472S.
The field cultivation and seed production test results show that: TY12472S has strong field growth vigor, strong tillering force, regular and consistent growth, shorter plant height, strong lodging resistance, high stigma exposure rate at high temperature, 100% of total stigma exposure rate, 75.7% of bilateral exposure rate, 42.6% of 63S stigma exposure rate which is wider than that of control, and 43.1% of bilateral exposure rate. The sterile line has strong high temperature resistance, is subjected to multi-combination trial production in the extremely high temperature of the Wuhan in 2013, has the outcrossing rate of 65 percent, has the average per mu yield of more than 250 kilograms, and has the average per mu yield of less than 50 kilograms in comparison with the wide range of 63S; sterile line is manually stripped and inspected, no abdomen is made, and chalky grain rate is 0.
Example 2
Compared with the cross-pollination of the Guangzhan 63S under the simple high-temperature stress setting of the TY12472S by using the figure 2,
and (3) comparing the setting rate:
statistical results show that: under the high temperature of 63S, the TY12472S and the control Guangdong are hybridized manually by using two male parents, the R1 male parent is hybridized, and the TY12472S ratio is 74.8% higher than the 63S fruiting rate; the hybridization of the R2 male parent and the TY12472S is 75.8 percent higher than the 63S seed setting rate, which shows that the TY12472S has strong high temperature resistance, strong column head activity at high temperature and good outcrossing property.
Example 3
The breeding process of two-excellent 4722:
in 2012, the light (temperature) sensitive nuclear male sterile line TY12472S of Hainan breeding is used as a female parent, the high-quality two-line restorer line R12 is used as a male parent for hybridization, the hybrid seeds are planted in the summer of 2012, the hybrid vigor of the hybrid seeds is observed, the hybrid seeds grow vigorously, the stalks are thick and strong, the ears are large in grain size, the maturing rate is high, the later color conversion is good, the rice quality is excellent, the manual stripping and inspection are carried out, the abdomen is white, the rice grain is slender, the same year is repeated, the hybrid vigor is observed in Hainan, the expression is outstanding, the Wuhan in 2013 is subjected to small-area combined test, the district quality comparison test is carried out in 2014, and the test result shows that the fertility period is equivalent to that of the Fengyou No. 4, the plant height is shorter than the comparison, the yield is increased by 3.4% and the rice quality and the resistance are superior to the comparison.
And all that is not described in detail in this specification is well known to those skilled in the art.
Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (5)
1. A method for breeding a rice high-temperature-resistant light (temperature) -sensitive nuclear male sterile line is characterized by comprising the following steps:
s1, adopting an artificial glume-cutting, emasculation and hybrid mating method, hybridizing by taking light (temperature) -sensitive nuclear male sterile line Xianshas as a female parent and martial arts B as a male parent to obtain an F1 generation, planting the F1 generation, mixedly collecting the F1 generation after maturation, planting an F2 generation group, and selecting a fertile single plant with high fruiting rate, short plant height and excellent rice quality in the F2 generation;
s2, planting the F3 single plant, and performing high-temperature stress treatment on the F3 single plant, and continuously selecting a fertile single plant with high fruiting rate, short plant height and good rice quality;
s3, selecting an F5 generation, selecting sterile single plants from the F5 generation, screening target characters of the sterile single plants, screening excellent single plants meeting the target characters, cutting stumps, regenerating, bagging and selfing, and harvesting regenerated sub-generation seeds;
s4, regenerating plant lines of plant first-generation single plants, numbering each plant in a tillering stage, dividing the plant lines into two plants, carrying out high-temperature stress treatment on one plant, bagging the plant lines for propagation, selecting excellent sterile single plants meeting target characters under the high-temperature treatment, particularly screening the character single plants with high exposed stigmas, and carrying out restorer hybridization test and allocation on the screened single plants;
s5, counting, hybridizing, measuring and preparing single plant setting percentage, selecting sterile single plants with high outcrossing setting percentage, and harvesting corresponding propagation single plant seeds;
s6, screening out the high temperature resistant rice light (temperature) sensitive nuclear male sterile line meeting the two-line sterile line standard according to the methods of the steps S4 and S5.
2. The method for breeding a rice high-temperature-resistant light (temperature) -sensitive nuclear male sterile line according to claim 1, which is characterized in that: in the S1, the F1 is obtained by hybridization with the light (temperature) sensitive nuclear male sterile line as a female parent and excellent conventional rice as a male parent.
3. The method for breeding a rice high-temperature-resistant light (temperature) -sensitive nuclear male sterile line according to claim 1, which is characterized in that: in the S3, the subsequent generation of the sterile plant is subjected to simple high Wen Penggao temperature stress, the restorer line is used for hybridization, the outcrossing seed setting rate is counted, and the high-temperature resistant light (temperature) sensitive nuclear male sterile line which accords with the breeding target is selected.
4. The method for breeding a rice high-temperature-resistant light (temperature) -sensitive nuclear male sterile line according to claim 1, which is characterized in that: the fertile single plant is bred from F2, F3-F4 is bred by simple high Wen Penggao temperature stress, and the selected single plant has good leaf shape, excellent rice quality, high outcrossing and fruiting and high temperature resistance at high temperature.
5. The method for breeding a rice high-temperature-resistant light (temperature) -sensitive nuclear male sterile line according to claim 1, which is characterized in that: f5, starting to select sterile plants, always utilizing simple high Wen Penggao temperature stress, hybridizing the sterile plants by using an external restoring line, counting the outcrossing seed setting rate after hybridization, selecting sterile single plants at high temperature, resisting high temperature, verifying high temperature resistance by utilizing the outcrossing seed setting rate, and selecting sterile plants with strong high temperature resistance, good outcrossing property, high temperature climate of the sterile lines and high seed production yield.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310371700.0A CN116406615A (en) | 2023-04-10 | 2023-04-10 | Method for breeding rice high-temperature-resistant light (temperature) -sensitive nuclear male sterile line |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310371700.0A CN116406615A (en) | 2023-04-10 | 2023-04-10 | Method for breeding rice high-temperature-resistant light (temperature) -sensitive nuclear male sterile line |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN116406615A true CN116406615A (en) | 2023-07-11 |
Family
ID=87059192
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310371700.0A Pending CN116406615A (en) | 2023-04-10 | 2023-04-10 | Method for breeding rice high-temperature-resistant light (temperature) -sensitive nuclear male sterile line |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN116406615A (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2011188744A (en) * | 2010-03-11 | 2011-09-29 | Tokyo Univ Of Science | High temperature-tolerant plant and screening method therefor |
| CN102884976A (en) * | 2012-08-14 | 2013-01-23 | 武汉大学 | Method for breeding hybrid rice photosensitive (thermosensitive) male sterile line |
| CN109797234A (en) * | 2018-12-03 | 2019-05-24 | 中国农业科学院深圳生物育种创新研究院 | With the molecular labeling R060939-2 of resistance gene of rice blast Pi2 close linkage |
| CN110692507A (en) * | 2018-07-09 | 2020-01-17 | 中国科学院遗传与发育生物学研究所 | Method for improving plant species |
| KR102142031B1 (en) * | 2020-02-03 | 2020-08-06 | 세종대학교산학협력단 | New rice variety 'Sebi' with increased environmental stress resistance and breeding method thereof |
| CN111955341A (en) * | 2020-08-10 | 2020-11-20 | 江苏沿海地区农业科学研究所 | Efficient breeding method of heat-resistant rice |
| CN112640776A (en) * | 2021-02-20 | 2021-04-13 | 湖南袁创超级稻技术有限公司 | Breeding of rice photo-thermo sensitive sterile line with high outcrossing rate and seed production method thereof |
-
2023
- 2023-04-10 CN CN202310371700.0A patent/CN116406615A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2011188744A (en) * | 2010-03-11 | 2011-09-29 | Tokyo Univ Of Science | High temperature-tolerant plant and screening method therefor |
| CN102884976A (en) * | 2012-08-14 | 2013-01-23 | 武汉大学 | Method for breeding hybrid rice photosensitive (thermosensitive) male sterile line |
| CN110692507A (en) * | 2018-07-09 | 2020-01-17 | 中国科学院遗传与发育生物学研究所 | Method for improving plant species |
| CN109797234A (en) * | 2018-12-03 | 2019-05-24 | 中国农业科学院深圳生物育种创新研究院 | With the molecular labeling R060939-2 of resistance gene of rice blast Pi2 close linkage |
| KR102142031B1 (en) * | 2020-02-03 | 2020-08-06 | 세종대학교산학협력단 | New rice variety 'Sebi' with increased environmental stress resistance and breeding method thereof |
| CN111955341A (en) * | 2020-08-10 | 2020-11-20 | 江苏沿海地区农业科学研究所 | Efficient breeding method of heat-resistant rice |
| CN112640776A (en) * | 2021-02-20 | 2021-04-13 | 湖南袁创超级稻技术有限公司 | Breeding of rice photo-thermo sensitive sterile line with high outcrossing rate and seed production method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 王明: ""水稻光温敏核不育系育性敏感期温度对异交特性的影响"", 《中国优秀硕士学位论文全文数据库 农业科技辑》, no. 10, pages 047 - 12 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US11246275B2 (en) | Method for breeding small-grain sterile rice line and simple method for producing hybrid rice seed | |
| CN107801631B (en) | Heat conduction temperature corn germplasm penetration type breeding improvement method | |
| CN107549006B (en) | Method for cultivating onion male sterile line and maintainer line | |
| CN108391588B (en) | Breeding method of three-line indica-japonica hybrid glutinous rice | |
| CN111280047A (en) | Breeding method of rice two-line sterile line with excellent performance | |
| CN116406615A (en) | Method for breeding rice high-temperature-resistant light (temperature) -sensitive nuclear male sterile line | |
| CN115633635A (en) | Method for creating sweet corn DH line based on haploid breeding technology | |
| CN112616651B (en) | Breeding method of glyphosate-resistant cotton genic male sterile dual-purpose line | |
| CN111406644B (en) | Cluster-growing pod pepper nuclear-cytoplasmic interaction type male sterile line breeding method and application of three-line matched system | |
| Taguchi-Shiobara et al. | Variation in domesticated rice inflorescence architecture revealed by principal component analysis and quantitative trait locus analysis | |
| YAMADA et al. | Production of interspecific hybrids between Trifolium ambiguum M. Bieb and T. repens L. by ovule culture | |
| CN111820124A (en) | Method for carrying out backcross breeding by using plant male sterile material and application | |
| CN108990794B (en) | Hybrid rice breeding method | |
| CN1065712C (en) | Hybrid vigor fixing breeding process for rice apomixis | |
| CN104871956A (en) | Hybrid rice production method | |
| CN113994886B (en) | Breeding method of ornamental and edible hybrid rice sterile line | |
| CN1041988C (en) | Breeding method of reversible dual-purpose male nucleus sterile line of rice | |
| CN1078041C (en) | Production of color hybrid cotton | |
| CN108668890B (en) | Method for improving correct recognition rate of corn haploid | |
| CN114532216B (en) | Breeding method of multi-marker dual-purpose hybrid rice sterile line | |
| CN111869562B (en) | Breeding method of high-quality small-grain-flavor japonica rice Wuxiangjing 113 | |
| CN114591967B (en) | Application of corn TCP gene in cross breeding | |
| CN113383702B (en) | Breeding method of hybrid rice between subspecies of japonica indica-free glutinous rice and subspecies of japonica indica-free glutinous rice in short growth period | |
| CN115644049A (en) | Method for improving rice indica-japonica hybrid seed setting rate | |
| CN1072888C (en) | Tech. for improving pureness of three incross rapeseed |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |