CN116391800A - Feed additive containing composite microbial inoculum and preparation method thereof - Google Patents
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Polymers & Plastics (AREA)
- Food Science & Technology (AREA)
- Biotechnology (AREA)
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- Animal Husbandry (AREA)
- Physiology (AREA)
- Molecular Biology (AREA)
- Health & Medical Sciences (AREA)
- Mycology (AREA)
- Botany (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention relates to the technical field of feeds, in particular to a feed additive containing a composite microbial inoculum and a preparation method thereof. The preparation method comprises the following steps: obtaining rabbit stomach content, filtering and separating to obtain dry matters and liquid; mixing the dry matter with glucose, honey and water to obtain a dry matter mixture; mixing the liquid with brown sugar, mel, glucose and water to obtain a liquid mixture; mixing rabbit stomach, traditional Chinese medicinal materials, purple garlic wine, pomace, dry matter mixture, liquid mixture, rice, malt, gluten, corn flour, white granulated sugar, brown sugar and water, controlling the water content to be 40-45%, and carrying out anaerobic fermentation in a closed environment. The feed additive containing the composite microbial inoculum has the advantages of wide raw material sources, good temperature tolerance, high strain activity and low application cost, can promote the decomposition of the feed and improve the digestion and absorption of livestock and poultry on the feed, thereby leading the weight gain speed of the livestock and poultry to be higher.
Description
Technical Field
The invention relates to the technical field of feeds, in particular to a feed additive containing a composite microbial inoculum and a preparation method thereof.
Background
Because livestock and poultry in the breeding industry are frequently ill and dead, the economic benefit of farmers is seriously influenced, and the normal development of the breeding industry is hindered. To solve this problem, researchers have developed various kinds of feed additives including chemical additives, herbal feed additives, and the like.
At present, various Chinese herbal medicine feed additives are disclosed in various patents, and the main purposes are to replace antibiotics, improve the immunity of livestock and poultry, improve the survival rate of livestock and poultry and promote the weight gain of livestock and poultry. However, for the Chinese herbal medicine feed additive which is required to be prepared through fermentation, the microorganisms and enzymes contained in the Chinese herbal medicine feed additive are sensitive to temperature, so that the product has poor stability and is not easy to store and transport under severe environments with severe heat or severe cold, and the action effect of the additive is also greatly reduced.
Therefore, how to provide a Chinese herbal medicine feed additive with good temperature tolerance and stable storage and difficult deterioration, so that the Chinese herbal medicine feed additive can effectively and stably play the effects of strengthening and tonifying in severe temperature environments, and the technical problem to be solved in the field is urgent.
Disclosure of Invention
The invention is particularly proposed to solve the above technical problems. Specifically:
the invention provides a preparation method of a feed additive containing a composite microbial inoculum, which comprises the following steps:
(1) Obtaining rabbit stomach content, filtering and separating to obtain dry matters and liquid;
mixing the dry matter with glucose, honey and water to obtain a dry matter mixture;
mixing the liquid with brown sugar, honey, glucose and water to obtain a liquid mixture;
(2) Mixing rabbit stomach, traditional Chinese medicinal materials, purple garlic wine, pomace, the dry matter mixture, the liquid mixture, rice, malt, gluten, corn flour, white granulated sugar, brown sugar and water, controlling the water content to be 40-45%, and carrying out anaerobic fermentation in a closed environment;
wherein, the chinese-medicinal material includes: dandelion, motherwort, plantain herb, dried orange peel, hawthorn, mulberry leaf, safflower, ganoderma lucidum spore powder, dwarf lilyturf tuber, chrysanthemum pistil, pseudo-ginseng flower, ginseng fibrous root, red ginseng, american ginseng, bupleurum root, eucommia bark, wild jujube thorn and papaya leaf;
the purple garlic wine is a liquid obtained by soaking purple garlic in white spirit;
the pomace is a mixture of crushed bananas, oranges, dried haws and sugarcanes.
The invention discovers that the feed additive containing the composite microbial inoculum prepared by the preparation method has good temperature tolerance, can have good storage stability in a high-temperature environment up to 60 ℃ and a low-temperature environment ranging from minus 40 ℃ to minus 50 ℃, does not cause obvious deterioration, and can adapt to different temperature environments, thereby efficiently and stably playing the health-improving and tonifying effects.
In the specific implementation process, bananas and oranges can be treated with skin.
Preferably, the anaerobic fermentation temperature is 25-30 ℃, and the fermentation time is 10-20 days.
The feed additive containing the composite microbial inoculum obtained by fermentation under the fermentation condition has better performance.
Preferably, the preparation method of the purple garlic wine comprises the following steps:
and soaking the purple garlic in the white spirit with the alcohol content of 50-60 degrees for 7-15 days.
The purple garlic wine prepared from the white spirit with the concentration can improve the synergistic effect of the purple garlic wine and other components, so that the temperature tolerance of the feed additive containing the composite microbial inoculum is further improved.
Preferably, in the step (1), the weight ratio of the dry matter to glucose, honey and water is (15-25): 1: (2-4): (6-10).
Preferably, in the step (1), the weight ratio of the liquid to the brown sugar, the honey, the glucose and the water is (20-30): (2-6): (2-4): 1: (8-12).
Preferably, in the step (2), the rabbit stomach, the traditional Chinese medicinal materials, the purple garlic wine, the pomace, the dry matter mixture, the liquid mixture, the rice, the malt, the gluten, the corn flour, the white granulated sugar and the brown sugar are mixed according to the weight ratio of (550-650): (440-480): (355-375): (2700 to 2800): (220-260): (80-90): (450-550): (90-110): (4800-5200): (1800-2200): (220-260): (720-760) mixing.
Preferably, in the traditional Chinese medicinal materials, dandelion, motherwort herb, plantain herb, dried orange peel, hawthorn, mulberry leaf, safflower, ganoderma lucidum spore powder, dwarf lilyturf tuber, chrysanthemum pistil, pseudo-ginseng flower, ginseng fibrous root, red ginseng, american ginseng, bupleurum root, eucommia bark, wild jujube thorn and papaya leaf are mixed according to the weight ratio of (90-110): (20-40): (5-15): (40-60): (20-40): (10-30): (10-20): (5-15): (20-40): (10-30): (5-15): (20-40): (10-20): (20-40): (10-30).
Preferably, the pomace is prepared by crushing banana, orange, dried hawthorn and sugarcane, and the weight ratio of (1.5-2.5) to (0.5-1.2): (0.1 to 0.5): (1.5-2.5) and mixing the above materials.
The feed additive containing the composite microbial inoculum prepared under the dosage condition of the preferable proportion has better comprehensive performance.
Preferably, before the content of the rabbit stomach is obtained, the rabbit stomach is placed in an aqueous solution of isatis root and soaked for 6-8 hours.
Preferably, the rice is selected from short-grain old rice, soaked, dried and crushed.
Further, the invention provides the feed additive containing the composite microbial agent, which is prepared by any embodiment.
The feed additive containing the composite microbial inoculum has wide application range and can be added into different livestock and poultry feeds such as pigs, cattle, chickens, sheep, ducks and the like.
Further, the invention also provides a feed which contains the feed additive containing the composite microbial inoculum prepared by any one of the embodiments.
Compared with the prior art, the invention has the beneficial effects that:
the feed additive containing the composite microbial inoculum has wide raw material sources, good temperature tolerance, stable storage and difficult deterioration, can effectively and stably play a health-care and tonifying effect in a severe temperature environment with relatively hot temperature (60 ℃ to 60 ℃) or relatively cold temperature (-40 ℃ to 50 ℃), and improves the immunity of livestock and poultry; in addition, the feed additive containing the composite microbial inoculum can achieve excellent health care and tonifying effects only by adding 10-15 jin of the feed additive into each ton of complete feed, and has high strain activity and low application cost; in addition, the feed additive containing the composite microbial inoculum can promote the decomposition of the feed and improve the digestion and absorption of livestock and poultry on the feed, so that the weight gain speed of the livestock and poultry is higher.
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the present invention more apparent, the technical solutions of the present invention will be clearly and completely described below, and it is apparent that the described embodiments are some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
The examples are not intended to identify the particular technology or conditions, and are either conventional or are carried out according to the technology or conditions described in the literature in this field or are carried out according to the product specifications. The reagents and instruments used, etc. are not identified to the manufacturer and are conventional products available for purchase by regular vendors.
The rabbit stomach in the following examples was derived from the beijing De Chuan meat rabbit farm; all tools used for cutting are soaked in potassium permanganate solution for disinfection. The producer must disinfect the whole body, and the producer is taken into the bacteria producing room to prepare the feed additive containing the composite bacterial agent.
Example 1
The embodiment provides a feed additive containing a composite microbial agent, which is prepared by the following steps:
1. raw material preparation
1. Cutting 3 rabbit stomachs, and soaking in boiled and cooled radix Isatidis water solution for 7 hr; filtering and separating by adopting a fine sand net to obtain dry matters and liquid; drying the rabbit stomach, and pulverizing for later use;
mixing 150g of dry matter with 7.5g of glucose, 22.5g of honey and 60g of water to prepare 240g of dry matter mixture;
mixing 50g of liquid with 8g of brown sugar, 5g of honey, 2g of glucose and 20g of water to prepare 85g of liquid mixture;
2. soaking 500g of short-grain aged rice for 24 hours, airing and crushing for standby;
3. respectively crushing and mixing 100g of dandelion, 30g of motherwort, 10g of plantain herb, 50g of dried orange peel, 30g of hawthorn, 20g of mulberry leaf, 15g of safflower, 10g of ganoderma lucidum spore powder, 30g of dwarf lilyturf tuber, 20g of chrysanthemum core, 20g of sanchi flower, 10g of ginseng fibrous, 10g of red ginseng, 10g of American ginseng, 30g of bupleurum, 15g of eucommia bark, 30g of wild jujube thorn and 20g of papaya leaf (dried) to obtain a traditional Chinese medicine mixture;
4. flattening Bulbus Allii with skin, soaking in 53 deg.C Chinese liquor for 10 days to obtain Bulbus Allii wine;
5. mixing 1000g of banana, 500g of orange, 250g of dried hawthorn and 1000g of sugarcane, and stirring to obtain pomace;
2. fermentation
Mixing 600g of the crushed rabbit stomach, 460g of the traditional Chinese medicinal material mixture, 365g of purple garlic wine, 2750g of pomace, 240g of dry matter mixture, 85g of liquid mixture, 500g of short-grain old rice, 100g of malt, 5000g of gluten, 2000g of corn flour, 250g of white granulated sugar and 750g of brown sugar, adding water to control the water content to be about 44%, and performing anaerobic fermentation for 20 days in a closed environment at 25-27 ℃ to prepare the feed additive containing the composite microbial inoculum.
Example 2
The present example provides a feed additive containing a complex microbial agent, the preparation method of which is different from that of example 1 only: anaerobic fermentation is carried out for 12 days in a closed environment with the temperature of 28-30 ℃.
Example 3
The present example provides a feed additive containing a complex microbial agent, the preparation method of which is different from that of example 1 only:
the purple garlic (with skin) is flattened and soaked in distilled spirit at 60 ℃ for 10 days to prepare the purple garlic wine.
Example 4
The present example provides a feed additive containing a complex microbial agent, the preparation method of which is different from that of example 1 only:
the purple garlic (with skin) is flattened and soaked in white spirit at 48 ℃ for 10 days to prepare the purple garlic wine.
Example 5
The present example provides a feed additive containing a complex microbial agent, the preparation method of which is different from that of example 1 only:
the purple garlic (with skin) is flattened and soaked in the white spirit at 68 ℃ for 10 days to prepare the purple garlic wine.
Comparative example 1
This comparative example provides a feed additive, the preparation method only differs from example 1 in that: the purple garlic wine is replaced by white wine with the same alcohol content.
Comparative example 2
This comparative example provides a feed additive, the preparation method only differs from example 1 in that: mixing 1000g of banana, 500g of orange, 250g of dried hawthorn and 1000g of apple, and stirring to obtain pomace.
Test examples
The feed additives prepared in the examples and the comparative examples are stored in a refrigerator at-45 ℃ and-20 ℃ and in an incubator at 40 ℃ and 60 ℃, a normal temperature storage group (stored at-25 ℃) is additionally arranged as a control, the feed additives stored at different temperatures are placed in room temperature for 1h before being used and restored to room temperature, 5kg of feed additives are added into each 1t of pig feed during use, and the feed additives are continuously placed in corresponding temperature environments for storage after the addition. The pig feed in this test example was a positive large complete pig feed.
Healthy pigs 144 with the weight similar to that of 50kg are selected, wherein the pigs 72 and the sows 72 are grouped, each group of pigs and sows accounts for half of each group, pig feeds added with the feed additives stored at different temperatures are used for feeding every day, feeds without any feed additives are set as control groups, the feeding time and the feeding amount of each group are kept the same, the test period is 2 months, the feeding condition is observed every day, after the test, the weight of the pigs is weighed, the average value is taken, and the specific test results are shown in table 1.
TABLE 1 statistical table of eating status and average daily gain
In addition, the rabbit stomach is sampled in a plurality of places in the south slaughter house and the north slaughter house in China, and the rabbit stomach is used in the preparation method of the invention, and a great deal of practice discovers that the rabbit stomach has good and consistent effects and has no obvious difference.
In a large number of practical processes, the invention also adopts pig feeds of different brands to match with the feed additive containing the composite microbial inoculum, and the effect advantages equivalent to the test examples can be obtained.
Finally, it should be noted that: the above embodiments are only for illustrating the technical solution of the present invention, and are not limiting; although the invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical scheme described in the foregoing embodiments can be modified or some technical features thereof can be replaced by equivalents; such modifications and substitutions do not depart from the spirit and scope of the technical solutions of the embodiments of the present invention.
Claims (10)
1. The preparation method of the feed additive containing the composite microbial inoculum is characterized by comprising the following steps:
(1) Obtaining rabbit stomach content, filtering and separating to obtain dry matters and liquid;
mixing the dry matter with glucose, honey and water to obtain a dry matter mixture;
mixing the liquid with brown sugar, honey, glucose and water to obtain a liquid mixture;
(2) Mixing rabbit stomach, traditional Chinese medicinal materials, purple garlic wine, pomace, the dry matter mixture, the liquid mixture, rice, malt, gluten, corn flour, white granulated sugar, brown sugar and water, controlling the water content to be 40-45%, and carrying out anaerobic fermentation in a closed environment;
wherein, the chinese-medicinal material includes: dandelion, motherwort, plantain herb, dried orange peel, hawthorn, mulberry leaf, safflower, ganoderma lucidum spore powder, dwarf lilyturf tuber, chrysanthemum pistil, pseudo-ginseng flower, ginseng fibrous root, red ginseng, american ginseng, bupleurum root, eucommia bark, wild jujube thorn and papaya leaf;
the purple garlic wine is a liquid obtained by soaking purple garlic in white spirit;
the pomace is a mixture of crushed bananas, oranges, dried haws and sugarcanes.
2. The preparation method of claim 1, wherein the anaerobic fermentation is carried out at a temperature of 25-30 ℃ for 10-20 days.
3. The preparation method of the purple garlic wine according to claim 1 or 2, wherein the preparation method of the purple garlic wine comprises the following steps:
and soaking the purple garlic in the white spirit with the alcohol content of 50-60 degrees for 7-15 days.
4. The preparation method according to claim 1, wherein in the step (1), the weight ratio of the dry matter to glucose, honey and water is (15-25): 1: (2-4): (6-10);
and/or the weight ratio of the liquid to the brown sugar, the honey, the glucose and the water is (20-30): (2-6): (2-4): 1: (8-12).
5. The preparation method of claim 1, wherein in the step (2), the rabbit stomach, the traditional Chinese medicinal materials, the purple garlic wine, the pomace, the dry matter mixture, the liquid mixture, the rice, the malt, the gluten, the corn flour, the white granulated sugar and the brown sugar are mixed according to the weight ratio of (550-650): (440-480): (355-375): (2700 to 2800): (220-260): (80-90): (450-550): (90-110): (4800-5200): (1800-2200): (220-260): (720-760) mixing.
6. The preparation method of claim 1, wherein the weight ratio of dandelion, motherwort herb, plantain herb, dried orange peel, hawthorn, mulberry leaf, safflower, ganoderma lucidum spore powder, dwarf lilyturf tuber, chrysanthemum pistil, pseudo-ginseng flower, ginseng fibrous seed, red ginseng, american ginseng, bupleurum root, eucommia bark, wild jujube thorn and papaya leaf is (90-110): (20-40): (5-15): (40-60): (20-40): (10-30): (10-20): (5-15): (20-40): (10-30): (5-15): (20-40): (10-20): (20-40): (10-30).
7. The preparation method of claim 6, wherein the pomace is prepared from (1.5-2.5) of banana, orange, dried hawthorn and sugarcane by the following weight ratio (0.5-1.2): (0.1 to 0.5): (1.5-2.5) and mixing the above materials.
8. The preparation method of claim 1, wherein the rabbit stomach is soaked in the aqueous solution of isatis root for 6-8 hours before the content of the rabbit stomach is obtained.
9. A feed additive comprising a composite microbial agent, characterized in that it is produced by the production method according to any one of claims 1 to 8.
10. A feed comprising the feed additive comprising the composite microbial agent of claim 9.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104605140A (en) * | 2015-02-13 | 2015-05-13 | 韩褆 | Energy-saving and environment-friendly type microorganism Chinese herbal medicine fermented feed and preparation method thereof |
| US20200016227A1 (en) * | 2017-03-30 | 2020-01-16 | Mootral Sa | Antimicrobial garlic compositions |
| CN115678781A (en) * | 2022-12-30 | 2023-02-03 | 绿烨锦源(北京)生物科技有限公司 | Microbial inoculum for animal culture fermentation bed and preparation method thereof |
| US20230083754A1 (en) * | 2021-08-25 | 2023-03-16 | AGENT Innovation Co., Ltd. | Probiotics separated from rabbit feces and food containing probiotics |
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2023
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104605140A (en) * | 2015-02-13 | 2015-05-13 | 韩褆 | Energy-saving and environment-friendly type microorganism Chinese herbal medicine fermented feed and preparation method thereof |
| US20200016227A1 (en) * | 2017-03-30 | 2020-01-16 | Mootral Sa | Antimicrobial garlic compositions |
| US20230083754A1 (en) * | 2021-08-25 | 2023-03-16 | AGENT Innovation Co., Ltd. | Probiotics separated from rabbit feces and food containing probiotics |
| CN115678781A (en) * | 2022-12-30 | 2023-02-03 | 绿烨锦源(北京)生物科技有限公司 | Microbial inoculum for animal culture fermentation bed and preparation method thereof |
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