CN116358942A - Sampling device for flow cytometer detection - Google Patents

Sampling device for flow cytometer detection Download PDF

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Publication number
CN116358942A
CN116358942A CN202310476074.1A CN202310476074A CN116358942A CN 116358942 A CN116358942 A CN 116358942A CN 202310476074 A CN202310476074 A CN 202310476074A CN 116358942 A CN116358942 A CN 116358942A
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China
Prior art keywords
flow cytometer
sampling device
sampling
pipe
suction
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Withdrawn
Application number
CN202310476074.1A
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Chinese (zh)
Inventor
赵玉洁
刘兰
朱绍玲
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Affiliated Hospital of Zunyi Medical University
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Affiliated Hospital of Zunyi Medical University
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Priority to CN202310476074.1A priority Critical patent/CN116358942A/en
Publication of CN116358942A publication Critical patent/CN116358942A/en
Withdrawn legal-status Critical Current

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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/02Devices for withdrawing samples
    • G01N1/10Devices for withdrawing samples in the liquid or fluent state
    • G01N1/14Suction devices, e.g. pumps; Ejector devices
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B08CLEANING
    • B08BCLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
    • B08B9/00Cleaning hollow articles by methods or apparatus specially adapted thereto 
    • B08B9/08Cleaning containers, e.g. tanks
    • B08B9/0804Cleaning containers having tubular shape, e.g. casks, barrels, drums
    • B08B9/0813Cleaning containers having tubular shape, e.g. casks, barrels, drums by the force of jets or sprays
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/02Devices for withdrawing samples
    • G01N1/10Devices for withdrawing samples in the liquid or fluent state
    • G01N1/14Suction devices, e.g. pumps; Ejector devices
    • G01N2001/1418Depression, aspiration

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Hydrology & Water Resources (AREA)
  • Dispersion Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Mechanical Engineering (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The utility model discloses a sampling device for detecting a flow cytometer, which relates to the technical field of flow cytometers and comprises a tube placing component and a sampling component; the pipe placing assembly comprises a bottom plate and a pipe rack rotatably assembled on the surface of the bottom plate; the sampling assembly comprises an upright post and a material sucking mechanism which are integrally connected with the bottom plate; the sucking mechanism comprises a sucking pipe fitting and a sucking bag fitting which are communicated with each other, and the sucking pipe fitting and any test tube are positioned on the same vertical line; the technical key points are as follows: the design puts tub subassembly and sampling subassembly and combines, utilizes actuating mechanism to drive and inhale the material mechanism and reciprocate to realize that the cell stoste of each test tube in the opposition pipe subassembly is extracted and is detected, set up the connecting plate that has the waste water groove on lifting cylinder, can wash the straw spare with the help of installing the clear water pump in the detection case after carrying out single sample, conveniently carry out the sample detection to the cell stoste in the next test tube and handle.

Description

Sampling device for flow cytometer detection
Technical Field
The utility model relates to the technical field of flow cytometry, in particular to a sampling device for flow cytometry detection.
Background
Flow cytometry is a device that automatically analyzes and sorts cells; the device can rapidly measure, store and display a series of important biophysical and biochemical characteristic parameters of dispersed cells suspended in liquid, and can sort out specified cell subsets according to a preselected parameter range; most flow cytometers are zero-resolution instruments that can only measure an index such as the total nucleic acid amount, total protein amount, etc. of a cell, but cannot identify and measure the amount of nucleic acid or protein at a specific site; that is, its detail resolution is zero.
The prior patent number is CN213239599U, the name is a sampling device for detecting a flow cytometer, the sampling device comprises a fixed table, a motor is installed at the top of the fixed table, the output pump of the motor is connected with a rotating shaft through a coupling, the top end of the rotating shaft is connected with a driving gear, the driving gear is meshed with an internal gear, an installation table is arranged at the top of the internal gear, a placing rack is arranged at the top of the installation table, and a plurality of groups of limiting holes are formed in the top of the placing rack; according to the utility model, the test tube is placed on the placing frame, the electric push rod is started, the mounting block moves downwards, the sampling tube stretches into the test tube, the conveying pump is started, cells in the test tube are pumped into the detection device from the conveying tube for detection, when different types of cells need to be extracted, the motor is started, the test tube on the placing frame rotates along with the driving gear and the internal gear under the cooperation of the driving gear, and when the test tube runs below the sampling tube, sampling detection work is repeated, so that the sampling efficiency is improved, and the requirement of a cell experiment is met.
However, in the implementation process of the technical scheme, at least the following technical problems are found:
the sampling device that sets up on traditional flow cytometer needs to be with the help of the delivery pump when carrying out suction to the cell stoste to the cost of whole sampling device has been increased to a certain extent, needs to carry out artifical washing to whole sampling tube after taking a sample to single in vitro cell stoste simultaneously, then just can carry out the subsampling, causes the inconvenience of sampling work.
Disclosure of Invention
The technical problems to be solved are as follows:
the utility model provides a sampling device for detecting a flow cytometer, which is characterized in that a tube placing component is designed to be combined with a sampling component, and a driving mechanism is utilized to drive a suction mechanism to move up and down, so that the purpose of extracting and detecting cell stock solution in each test tube in the opposite tube component is achieved, a connecting plate with a waste water tank is arranged on a lifting cylinder, a suction tube piece can be washed by a clean water pump arranged in a detection box after single sampling is carried out, the sampling detection treatment of the cell stock solution in the next test tube is facilitated, and the technical problems mentioned in the background art are solved.
The technical scheme is as follows:
in order to achieve the above purpose, the utility model is realized by the following technical scheme:
a sampling device for detecting a flow cytometer comprises a tube-placing component and a sampling component;
the pipe placing assembly comprises a bottom plate and a pipe rack rotatably assembled on the surface of the bottom plate;
the sampling assembly comprises an upright post and a material sucking mechanism which are integrally connected with the bottom plate;
the test tube rack is internally provided with a plurality of test tubes which are distributed in an annular mode, the suction mechanism comprises a suction pipe piece and a suction bag piece which are communicated with each other, the suction pipe piece and any test tube are positioned on the same vertical line, the surface of the upright post is provided with a driving mechanism for driving the suction pipe piece, the suction bag piece is communicated with a detection box arranged on the back side of the upright post, and the suction bag piece at the position can synchronously move along with the suction pipe piece.
In one possible implementation manner, the pipe rack comprises upper disc pieces and lower disc pieces which are distributed up and down and are integrally connected, the upper disc pieces and the lower disc pieces can be driven to synchronously move, and meanwhile, the upper disc pieces and the lower disc pieces can be connected through a spiral sleeve, so that the distance between the upper disc pieces and the lower disc pieces is changed to cope with test tubes with different lengths;
the surfaces of the upper disc piece and the lower disc piece are provided with a plurality of round openings for inserting corresponding test tubes; a notch is formed in the position, located between two adjacent groups of round openings, of the surface of the lower disc piece; the rotary motor is installed to the bottom of bottom plate, and the supporting output shaft end of rotary motor runs through the bottom plate to be connected with the carousel, carousel surface welding pin, a plurality of breach confession pin rotation type entering on lower disk surface.
Above-mentioned rotating electrical machines drive carousel rotation, and carousel rotation is a round then drives the pin rotation round, and at this in-process pin can stir lower dish spare for inhale the bottom of pipe fitting towards the opening part of next test tube, so the circulation can realize carrying out the sample to the cell stoste in each test tube and handling
In one possible implementation, the driving mechanism includes a lifting cylinder and a sliding rail;
the lifting cylinder is arranged at the side of the upright post and is used for driving the sliding piece to move up and down, so that the suction pipe fitting also moves up and down synchronously;
the sliding rail is arranged at the side of the upright post, and the inner cavity of the sliding rail is used for a piston rod matched with the lifting cylinder to pass through; the sliding rail surface sliding type is provided with a sliding part, the suction pipe part is fixedly arranged on the surface of the sliding part, a suction port at the bottom end of the suction pipe part can be inserted into any test tube cavity, and the suction pipe part at the position can enter into each test tube cavity in the using process, so that batch sampling operation is completed.
In one possible implementation manner, the bottom end of the suction bag member is fixedly arranged on the surface of the sliding rail, the top end of the suction bag member is spirally connected with the sliding member, and the expansion direction of the suction bag member is consistent with the movement direction of the sliding member; the suction bag piece is connected with the detection box through a rubber tube, the suction bag piece is always positioned in the upper area of the detection box, the bottom end of the suction bag piece is fixed, and the telescopic movement of the whole suction bag piece depends on the movement of the sliding piece on the sliding rail.
In one possible implementation mode, a connecting plate is welded on the outer wall of the lifting cylinder, a through hole for a suction pipe to pass through is formed in the surface of the connecting plate, a blocking cover is arranged on the through hole, the blocking cover is connected with the connecting plate through a rotating shaft with a torsion spring, and the torsion spring drives the blocking cover to complete blocking treatment of the through hole, so that waste water is prevented from seeping out from the through hole; the bottom side of the connecting plate is provided with a waste water tank, the upper surface of the connecting plate is provided with water tanks which are distributed in an inclined mode, and the water tanks are used for communicating the communication port and the waste water tank; an instrument for detecting cells is arranged in the detection box.
Specifically, the lifting cylinder can drive the sliding part and the suction pipe part to move upwards synchronously until the state shown in fig. 1 is presented, the suction pipe part can be driven to move downwards and enter into the test tube, the suction bag part is in a compressed state in the downwards moving process, then the suction bag part is moved upwards, the suction treatment of the cell stock solution in the corresponding test tube is completed, until the suction pipe part is separated from the corresponding test tube, the rotating motor drives the turntable to rotate for one circle at the moment, the next test tube is accurately ready, the cell stock solution in the suction pipe part enters into the suction bag part, and then enters into the detection box through the rubber tube, and the subsequent detection work can be completed
The beneficial effects are that:
in the scheme, the tube placing assembly and the sampling assembly are combined, and the driving mechanism is used for driving the material sucking mechanism to move up and down, so that the cell stock solution in each test tube in the opposite tube assembly is extracted and detected, only the suction bag piece is needed, the traditional material pump design is not needed, the sampling detection treatment on various cell stock solutions is convenient, and the flexibility of the overall design is reflected;
in this scheme, through the connecting plate that sets up to have the waste water groove on lifting cylinder, can wash the straw spare with the help of installing the clear water pump in detecting the incasement after carrying out single sample, conveniently carry out sample detection to the cell stoste in the next test tube and handle, the waste water that produces can enter into the waste water inslot and accomplish and collect, can realize then detecting in batches the real-time of multiple cell stoste.
Drawings
The foregoing description is only an overview of the present utility model, and is intended to provide a better understanding of the present utility model, as it is embodied in the following description, with reference to the preferred embodiments of the present utility model and the accompanying drawings.
FIG. 1 is a schematic diagram of the overall structure of the present utility model;
FIG. 2 is a schematic view of a tube assembly according to the present utility model;
FIG. 3 is a schematic view of the lower disc member of the present utility model;
FIG. 4 is a schematic diagram of a sampling assembly according to the present utility model;
FIG. 5 is a schematic view of a suction pipe fitting according to the present utility model;
fig. 6 is a schematic view of a connecting plate structure according to the present utility model.
Legend description: 1. a bottom plate; 2. a column; 3. a turntable; 31. a stop lever; 4. a rotating electric machine; 5. a lower disc member; 6. a disc feeding piece; 7. a suction pipe fitting; 8. a slider; 9. a lifting cylinder; 10. a detection box; 11. a connecting plate; 12. a waste water tank; 13. a blocking cover; 14. a through port; 15. a water tank; 16. a suction bag piece.
Detailed Description
According to the sampling device for flow cytometry detection, the pipe placing assembly and the sampling assembly are combined, the driving mechanism is used for driving the material sucking mechanism to move up and down, so that cell stock solution in each test tube in the opposite pipe assembly is extracted and detected, the lifting cylinder is provided with the connecting plate with the waste water tank, after single sampling, the suction pipe piece can be washed by means of the clear water pump arranged in the detection box, sampling detection treatment is conveniently carried out on the cell stock solution in the next test tube, and the technical problem mentioned in the background art is solved.
The technical scheme in the embodiment of the application aims to solve the problems of the background technology, and the overall thought is as follows:
example 1:
the embodiment describes a specific structure of a sampling device for detecting a flow cytometer, as shown in fig. 1-6, and the sampling device comprises a tube placing component and a sampling component;
the tube placing assembly comprises a bottom plate 1 and a tube rack rotatably assembled on the surface of the bottom plate 1 for placing test tubes;
the sampling assembly comprises an upright post 2 and a sucking mechanism which are integrally connected with the bottom plate 1, and performs sucking treatment on cell stock solution in the test tube;
wherein, the pipe support is equipped with a plurality of test tubes that are annular distribution in the interpolation, and inhale material mechanism contains straw piece 7 and inhaling bag piece 16 that communicate each other, and straw piece 7 is in same vertical line with arbitrary test tube, and stand 2 surface mounting is used for driving the actuating mechanism who inhales pipe piece 7, inhales bag piece 16 and sets up in the detection case 10 of stand 2 dorsal part and be linked together, can install the flow cytometer in this department detection case 10, from setting up the container and for the entering of the cell stoste of suction to accomplish subsequent detection work.
In some examples, the pipe frame comprises an upper disc 6 and a lower disc 5 which are distributed up and down and are integrally connected, wherein the lower disc 5 has the same specification as the upper disc 6, and the structural design is different;
wherein, the surfaces of the upper disc member 6 and the lower disc member 5 are provided with a plurality of round openings for inserting corresponding test tubes; a notch is formed in the position, between two adjacent groups of round openings, of the surface of the lower disc piece 5; the rotating motor 4 is installed at the bottom of the bottom plate 1, and the end head of an output shaft matched with the rotating motor 4 penetrates through the bottom plate 1 and is connected with the turntable 3, the surface of the turntable 3 is welded with the stop lever 31, and a plurality of notches on the surface of the lower disc piece 5 are used for the stop lever 31 to enter in a rotating mode.
The specific use is as follows: the rotating motor 4 drives the rotary table 3 to rotate, the rotary table 3 rotates for a circle to drive the stop lever 31 to rotate for a circle, the stop lever 31 can stir the lower disc piece 5 in the process, the bottom end of the suction pipe piece 7 faces the opening part of the next test tube, and the sampling treatment of the cell stock solution in each test tube can be realized through the circulation.
In some examples, the drive mechanism comprises a lifting cylinder 9 and a slide rail;
the lifting cylinder 9 is arranged at the side of the upright post 2 and is used for driving the suction pipe fitting 7 to move up and down;
the sliding rail is arranged at the side of the upright post 2, and the inner cavity of the sliding rail is used for a piston rod matched with the lifting cylinder 9 to pass through; the sliding rail surface is slidably provided with a sliding part 8, the suction pipe part 7 is fixedly arranged on the surface of the sliding part 8, a suction port at the bottom end of the suction pipe part 7 can be inserted into any test tube cavity, and the suction treatment of cell stock solution can be realized after the suction pipe part 7 enters a corresponding test tube.
In some examples, the bottom end of the suction bag member 16 is fixedly arranged on the surface of the sliding rail, the top end of the suction bag member 16 is in spiral connection with the sliding member 8, and the telescopic direction of the suction bag member 16 is consistent with the moving direction of the sliding member 8; the suction bag member 16 is connected with the detection box 10 through a rubber tube, and the suction bag member 16 is always positioned in the upper area of the detection box 10.
When the device specifically works, the lifting cylinder 9 can drive the sliding part 8 and the suction pipe part 7 to move upwards until the device is in the state shown in fig. 1, can drive the suction pipe part 7 to move downwards and enter into a test tube, the suction bag part 16 is in a compressed state in the downwards moving process, and then moves upwards to enable the suction bag part 16 to complete the suction treatment of cell stock solution in a corresponding test tube until the suction pipe part 7 is separated from the corresponding test tube, at the moment, the rotating motor 4 drives the rotary table 3 to rotate for a circle, so that the next test tube is accurately ready, the cell stock solution sucked into the suction bag part 7 enters into the detection box 10 through a rubber tube, and then the subsequent detection work can be completed.
By adopting the technical scheme:
the above-mentioned design puts pipe assembly and sampling subassembly and combines, utilizes actuating mechanism to drive and inhales material mechanism and reciprocate to realize that the cell stoste in each test tube in the opposition pipe assembly is extracted and is detected, only need with the help of inhale bag piece 16 can, need not with the help of traditional charge pump design, conveniently sample detection handles various cell stoste, embodied overall design's flexibility.
Example 2:
based on embodiment 1, this embodiment describes a specific structure of a connection plate in a sampling device for flow cytometer, as shown in fig. 1 and 6, a connection plate 11 is welded on an outer wall of a lifting cylinder 9, a through hole 14 through which a suction pipe 7 passes is formed on a surface of the connection plate 11, a blocking cover 13 is disposed on the through hole 14, and the blocking cover 13 is connected with the connection plate 11 through a rotating shaft with a torsion spring.
The suction pipe piece 7 moves upwards after one-time circulation, the suction pipe piece 7 passes through the through hole 14, the blocking cover 13 at the moment is used for blocking the through hole 14, then a clean water pump in the detection box 10 is driven by a PLC226 type controller externally connected with the suction pipe to be communicated with a rubber pipe in the detection box 10, the operation is switched to flushing operation, so that the clean water takes away redundant cell stock solution in the suction bag piece 16 and the suction pipe piece 7, the next test tube sampling treatment is conveniently carried out, and then the part of waste water passes through the through hole 14 and the water tank 15 and finally enters the waste water tank 12 to be collected.
In some examples, the bottom side of the connection plate 11 is provided with a waste water tank 12, and the upper surface of the connection plate 11 is provided with water tanks 15 distributed in an inclined manner, the water tanks 15 are used for communicating the communication port 14 with the waste water tank 12, and the waste water tank 12 is used for collecting redundant waste water.
In some examples, an instrument for detecting cells is disposed within the detection box 10.
By adopting the technical scheme:
the lifting cylinder 9 is provided with the connecting plate 11 with the waste water tank 12, after single sampling, the suction pipe piece 7 can be washed by means of the clean water pump arranged in the detection box 10, so that the cell stock solution in the next test tube can be conveniently sampled, detected and treated, and the generated waste water can enter the waste water tank 12 to be collected, so that real-time batch detection of various cell stock solutions can be realized.
Finally, it should be noted that: it is apparent that the above examples are only illustrative of the present utility model and are not limiting of the embodiments. Other variations or modifications of the above teachings will be apparent to those of ordinary skill in the art. It is not necessary here nor is it exhaustive of all embodiments. And obvious variations or modifications thereof are contemplated as falling within the scope of the present utility model.

Claims (10)

1. A sampling device for flow cytometer detection, comprising:
the pipe placing assembly comprises a bottom plate (1) and a pipe rack rotationally assembled on the surface of the bottom plate (1); and
the sampling assembly comprises an upright post (2) and a material sucking mechanism which are integrally connected with the bottom plate (1);
wherein, a plurality of test tubes that are annular distribution are equipped with in the pipe support, inhale material mechanism and contain straw spare (7) and the inhaling bag spare (16) of intercommunication each other, and straw spare (7) are in same vertical line with arbitrary test tube, stand (2) surface mounting is used for driving the actuating mechanism who inhales pipe spare (7), inhale bag spare (16) and set up in detection case (10) of stand (2) dorsal part and be linked together.
2. The sampling device for flow cytometer detection according to claim 1, wherein: the pipe frame comprises an upper disc (6) and a lower disc (5) which are distributed up and down and are integrally connected;
wherein, the surfaces of the upper disc (6) and the lower disc (5) are provided with a plurality of round openings for inserting corresponding test tubes; and a notch is formed in the position, between two adjacent groups of round openings, of the surface of the lower disc piece (5).
3. A sampling device for flow cytometer detection as described in claim 2, wherein: the rotary motor (4) is installed to the bottom of bottom plate (1), and the supporting output shaft end of rotary motor (4) runs through bottom plate (1) to be connected with carousel (3), carousel (3) surface welding pin (31), a plurality of breach confession pin (31) rotation type of lower dish spare (5) surface get into.
4. The sampling device for flow cytometer detection according to claim 1, wherein: the driving mechanism comprises a lifting cylinder (9) and a sliding rail;
wherein the lifting cylinder (9) is arranged at the side of the upright post (2);
the sliding rail is arranged at the side of the upright post (2), and the inner cavity of the sliding rail is used for a piston rod matched with the lifting cylinder (9) to pass through.
5. The sampling device for flow cytometer detection according to claim 4, wherein: the sliding rail surface sliding type is provided with a sliding part (8), the suction pipe part (7) is fixedly arranged on the surface of the sliding part (8), and a suction port at the bottom end of the suction pipe part (7) can be inserted into any test tube cavity.
6. The sampling device for flow cytometer detection according to claim 4, wherein: the bottom end of the suction bag piece (16) is fixedly arranged on the surface of the sliding rail, the top end of the suction bag piece (16) is spirally connected with the sliding piece (8), and the expansion direction of the suction bag piece (16) is consistent with the moving direction of the sliding piece (8).
7. The sampling device for flow cytometer detection according to claim 1, wherein: the suction bag piece (16) is connected with the detection box (10) through a rubber tube, and the suction bag piece (16) is always positioned in the area above the detection box (10).
8. The sampling device for flow cytometer detection according to claim 4, wherein: the outer wall of the lifting cylinder (9) is welded with a connecting plate (11), a through hole (14) for the suction pipe (7) to pass through is formed in the surface of the connecting plate (11), a blocking cover (13) is arranged on the through hole (14), and the blocking cover (13) is connected with the connecting plate (11) through a rotating shaft with a torsion spring.
9. The flow cytometer detection sampling apparatus as described in claim 8, wherein: the bottom side of connecting plate (11) is equipped with waste water groove (12), and connecting plate (11) upper surface sets up be basin (15) of slope distribution, basin (15) are used for communicating opening (14) and waste water groove (12).
10. The sampling device for flow cytometer detection according to claim 1, wherein: an instrument for detecting cells is arranged in the detection box (10).
CN202310476074.1A 2023-04-28 2023-04-28 Sampling device for flow cytometer detection Withdrawn CN116358942A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202310476074.1A CN116358942A (en) 2023-04-28 2023-04-28 Sampling device for flow cytometer detection

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202310476074.1A CN116358942A (en) 2023-04-28 2023-04-28 Sampling device for flow cytometer detection

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CN116358942A true CN116358942A (en) 2023-06-30

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117244706A (en) * 2023-09-18 2023-12-19 浙江基预科技有限公司 Single-cell controllable detection device and detection method thereof

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117244706A (en) * 2023-09-18 2023-12-19 浙江基预科技有限公司 Single-cell controllable detection device and detection method thereof

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Application publication date: 20230630