CN116337771A - Blood analysis device and method - Google Patents

Abstract

The invention provides a blood analysis device and a method, when detecting a specific item, a reaction tank controls an optical device to obtain an optical signal of a sample to be tested in the reaction tank in the process of cyclone mixing so as to obtain a reaction curve of a whole blood sample and a reagent; and determining the time length spent by the reaction curve tending to stabilize, and determining that the reaction tank is abnormal in mixing if the time length spent by the reaction curve tending to stabilize is longer than the preset time length. According to the blood analysis device, the reaction curve of the reaction tank in the cyclone mixing process is detected, whether the reaction tank is abnormal in mixing or not is determined based on the reaction curve, and when the abnormal mixing of the reaction tank is determined, a corresponding treatment strategy is timely adopted to prevent risks and harm and avoid outputting inaccurate detection results.

Description

Blood analysis device and method

Technical Field

The present disclosure relates to the field of in vitro testing, and in particular to a blood analysis device and method.

Background

The blood cell analyzer is a detection instrument commonly used in clinic, and can finish operations such as sorting and counting blood cells, so as to output measurement parameters such as white blood cells, red blood cells, platelets and the like with clinical guidance significance. The analyzer is generally designed with a blood sample supply part, a reagent supply part, a reaction tank and a measuring part, wherein the blood sample supply part and the reagent supply part respectively supply a blood sample and a reagent to the reaction tank, the blood sample and the reagent are uniformly mixed in the reaction tank to obtain a sample to be tested, and then the measuring part detects the sample to be tested to obtain detection results, namely various measurement parameters.

The rotational flow mixing is a method for mixing the blood sample and the reagent in the reaction tank. The rotational flow mixing is to mix the reagent with the blood sample by means of rotational flow generated by adding the reagent into the reaction tank. When the liquid feeding pipeline of the reagent supply part is blocked, the liquid feeding flow deviation is possibly caused, the cyclone mixing is abnormal, and the final detection result is inaccurate. Therefore, it is very important to provide a method for monitoring whether abnormal cyclone mixing occurs in the reaction tank.

Disclosure of Invention

Based on the shortcomings of the prior art, the invention provides a blood analysis device and a blood analysis method, so as to more accurately detect whether the cyclone mixing abnormality occurs in a reaction tank.

In order to achieve the above object, the embodiment of the present invention provides the following technical solutions:

according to a first aspect, there is provided in one embodiment a blood analysis device comprising: a sampling unit, a reagent supply unit, a detection unit, and a processor;

the sampling part is used for drawing a whole blood sample and conveying the whole blood sample to the detection part;

the reagent supply part is used for conveying the reagent to the detection part;

the detection part comprises a reaction tank and an optical device, wherein the reaction tank is used for receiving the whole blood sample conveyed by the sampling part and the reagent conveyed by the reagent supply part to prepare a sample to be detected; the optical device comprises a light emitting end and a light detecting end, wherein the light emitting end is used for emitting light capable of irradiating the to-be-detected sample in the reaction tank, and the light detecting end is used for receiving the light passing through the to-be-detected sample in the reaction tank so as to detect specific items;

The processor, wherein:

the processor is further configured to control the optical device to acquire an optical signal of the sample to be measured in the reaction cell to acquire a reaction curve of the whole blood sample and the reagent when controlling the reagent supply section to deliver the reagent to the reaction cell to cause the whole blood sample and the reagent to relatively flow to prepare the sample to be measured when the detection of the specific item is performed;

the processor determines the time length spent by the reaction curve tending to stabilize, and determines that the reaction tank is abnormal in mixing if the time length spent by the reaction curve tending to stabilize is longer than a preset time length; wherein the response curve tends to stabilize such that the fluctuation amplitude of the response curve is within a preset amplitude range.

In some embodiments, the processor is specifically configured to: determining the time period taken for the reaction curve to stabilize and determining the standard deviation of each data point on the reaction curve;

if the time spent by the reaction curve tending to stabilize is longer than the preset time and the standard deviation is greater than the standard deviation threshold, determining that the reaction tank is abnormal in mixing.

In some embodiments, the processor is specifically configured to: the optical signal is converted to an electrical signal and the response curve is determined based on the electrical signal.

In some embodiments, the detection of the specific item comprises detection of a specific protein, the reaction cell comprises a first reaction cell, the optics comprise a first optics, and the processor is specifically configured to:

when specific protein detection is performed, the sampling part is controlled to draw a first whole blood sample and convey the first whole blood sample to the first reaction tank, and the reagent supply part is controlled to convey a first hemolytic agent to the first reaction tank so as to prepare a first sample to be tested, wherein the processor is further used for controlling the first optical device to acquire a first optical signal of the first sample to be tested in the first reaction tank so as to acquire a reaction curve of the first whole blood sample and the first hemolytic agent when the reagent supply part is controlled to convey the first hemolytic agent to the first reaction tank so as to enable the first whole blood sample and the first hemolytic agent to relatively flow to prepare the first sample to be tested.

In some embodiments, the processor is further configured to:

if the time spent by the reaction curve tending to stabilize is not longer than the preset time, determining that the first reaction tank is not abnormal in mixing;

controlling the reagent supply part to convey a latex reagent into the first reaction tank to react with the first sample to be tested to prepare a specific protein detection sample under the condition that the first reaction tank is determined not to have abnormal mixing, and controlling the first optical device to acquire a second optical signal of the specific protein detection sample in the first reaction tank; and obtaining the concentration of the specific protein in the first whole blood sample according to the second optical signal.

In some embodiments, the detection of the particular item comprises hemoglobin detection, the reaction cell comprises a second reaction cell, the optics comprise a second optics, and the processor is configured to:

when hemoglobin detection is performed, the sampling part is controlled to draw a second whole blood sample and convey the second whole blood sample to the second reaction tank, and the reagent supply part is controlled to convey a diluent to the second reaction tank to prepare a second test sample, wherein the processor is further used for controlling the second optical device to acquire a second optical signal of the second test sample in the second reaction tank to acquire a reaction curve of the second whole blood sample and the diluent when the reagent supply part is controlled to convey the diluent to the second reaction tank to enable the second whole blood sample and the diluent to flow relatively to prepare the second test sample.

In some embodiments, the processor is further configured to:

if the time spent by the reaction curve tending to stabilize is not longer than the preset time, determining that the second reaction tank is not abnormal in mixing;

and under the condition that the second reaction tank is not subjected to uniform mixing abnormality, controlling the reagent supply part to convey a second hemolytic agent into the second reaction tank to react with the second to-be-detected sample so as to prepare a hemoglobin detection sample, and controlling the second optical device to acquire a third optical signal of the hemoglobin detection sample in the second reaction tank so as to acquire the hemoglobin concentration in the second whole blood sample.

According to a second aspect, there is provided in one embodiment a blood analysis device comprising: a sampling unit, a reagent supply unit, a detection unit, and a processor;

the sampling part is used for drawing a whole blood sample and conveying the whole blood sample to the detection part;

the reagent supply part is used for conveying the reagent to the detection part;

the detection part comprises a reaction tank and an optical device, wherein the reaction tank is used for receiving the whole blood sample conveyed by the sampling part and the reagent conveyed by the reagent supply part to prepare a sample to be detected; the optical device comprises a light emitting end and a light detecting end, wherein the light emitting end is used for emitting light capable of irradiating the to-be-detected sample in the reaction tank, and the light detecting end is used for receiving the light passing through the to-be-detected sample in the reaction tank so as to detect specific items;

the processor, wherein:

the processor is further configured to control the optical device to acquire an optical signal of the sample to be measured in the reaction cell to acquire a reaction curve of the whole blood sample and the reagent when controlling the reagent supply section to deliver the reagent to the reaction cell to cause the whole blood sample and the reagent to relatively flow to prepare the sample to be measured when the detection of the specific item is performed;

The processor determines whether the reaction curve tends to be stable within a preset time period; if the reaction curve does not tend to be stable within the preset time, determining that the reaction tank is abnormal in uniform mixing; wherein the response curve tends to stabilize such that the fluctuation amplitude of the response curve is within a preset amplitude range.

According to a third aspect, there is provided in one embodiment a method of blood analysis, the method comprising:

when a reagent is conveyed to a reaction tank so that a whole blood sample and the reagent relatively flow to prepare a to-be-tested sample, acquiring an optical signal of the to-be-tested sample in the reaction tank so as to acquire a reaction curve of the whole blood sample and the reagent;

determining the length of time it takes for the reaction curve to stabilize;

if the time spent by the reaction curve tending to stabilize is longer than a preset time, determining that the reaction tank is abnormal in uniform mixing;

wherein the response curve tends to stabilize such that the fluctuation amplitude of the response curve is within a preset amplitude range.

According to a fourth aspect, there is provided in one embodiment a method of blood analysis, the method comprising:

when a reagent is conveyed to a reaction tank so that a whole blood sample and the reagent relatively flow to prepare a to-be-tested sample, acquiring an optical signal of the to-be-tested sample in the reaction tank so as to acquire a reaction curve of the whole blood sample and the reagent;

Determining whether the reaction curve tends to be stable within a preset time period;

if the reaction curve does not tend to be stable within the preset time, determining that the reaction tank is abnormal in uniform mixing;

wherein the response curve tends to stabilize such that the fluctuation amplitude of the response curve is within a preset amplitude range.

According to a fifth aspect, an embodiment provides a computer readable storage medium comprising a program executable by a processor to implement the blood analysis method described in any of the embodiments herein.

From the above technical solutions, the embodiment of the present invention has the following advantages:

by detecting the reaction curve of the reaction tank in the cyclone mixing process and determining whether the reaction tank is abnormal in mixing or not based on the reaction curve, a corresponding treatment strategy is adopted in time to prevent risks and harm when the abnormal mixing of the reaction tank is determined, and inaccurate detection results are prevented from being output.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are required to be used in the embodiments or the description of the prior art will be briefly described below, and it is obvious that the drawings in the following description are only embodiments of the present invention, and that other drawings can be obtained according to the provided drawings without inventive effort for a person skilled in the art.

FIG. 1 is a schematic diagram of a blood analysis device according to an embodiment of the present invention;

FIG. 2 is a schematic diagram of another embodiment of a blood analysis device according to the present invention;

FIG. 3 is a schematic structural diagram of a measurement channel according to an embodiment of the present invention;

FIG. 4 is an exemplary graph of a plurality of reaction curves provided by an embodiment of the present invention;

FIG. 5 is a flow chart of a blood analysis method according to an embodiment of the present invention;

fig. 6 is another flowchart of a blood analysis method according to an embodiment of the present invention.

Detailed Description

The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.

Finally, it is further noted that relational terms such as first and second, and the like are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Moreover, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising one … …" does not exclude the presence of other like elements in a process, method, article, or apparatus that comprises the element.

The cyclone mixing refers to a method for mixing a blood sample and a reagent in a reaction tank, namely, the reagent is added into the reaction tank to generate cyclone so as to mix the cyclone with the blood sample. However, if the liquid feeding line of the reagent supply portion is clogged, the cyclone mixing becomes abnormal, and the final detection result becomes inaccurate. Therefore, it is very important to provide a method for monitoring whether abnormal cyclone mixing occurs in the reaction tank.

The inventor finds that by detecting the reaction curve of the reaction tank in the cyclone mixing process and determining whether the reaction tank is abnormal in mixing according to the reaction curve, the abnormal mixing of the reaction tank can be accurately and timely found, so that a corresponding treatment strategy is adopted to prevent risks and harm from occurring, and inaccurate detection results are prevented from being output.

By applying some embodiments of the present invention, the actual application scenario is as follows: when the reagent supply part is controlled to convey the reagent to the reaction tank so that the whole blood sample and the reagent relatively flow to prepare a sample to be tested (namely, a rotational flow mixing process), the optical device is controlled to acquire an optical signal of the sample to be tested in the reaction tank so as to acquire a reaction curve of the whole blood sample and the reagent. And determining whether the reaction tank is abnormal in mixing or not according to the obtained reaction curve. When the abnormal mixing of the reaction tank is determined, processing strategies such as alarm information output and detection result shielding are adopted, so that risks and harm can be prevented, and inaccurate detection results can be prevented from being output.

The present invention will be described in detail below with reference to examples.

Some embodiments of the invention disclose a blood analysis device. Referring to fig. 1, the blood analysis device in some embodiments may include a sampling part 10, a reagent supplying part 20, a detecting part 30, and a processor 40. Specifically, the sampling section 10 is for drawing a whole blood sample, and delivering the whole blood sample to the detecting section 30; the reagent supply unit 20 is configured to deliver a reagent to the detection unit 30; the detection unit 30 is used for detecting a specific item, such as a specific protein and hemoglobin.

From a structural point of view, referring to fig. 2, fig. 2 is a schematic diagram of another structure of the blood analysis device, and the detecting portion 30 may include a reaction tank 31 and an optical device 32; the reaction cell 31 is for receiving the whole blood sample supplied from the sampling section 10 and the reagent supplied from the reagent supplying section 20 to prepare a sample to be measured; the optical device 32 includes a light emitting end for emitting light that irradiates the sample to be measured in the reaction cell, and a light detecting end for receiving light that passes through the sample to be measured in the reaction cell to perform detection of a specific item.

In some embodiments, the sampling portion 10 may include a sample needle that is moved in two or three dimensions by a two or three dimensional drive mechanism so that the sample needle may be moved to aspirate a whole blood sample in a container (e.g., a sample container) carrying the whole blood sample and then moved to a reaction site, such as a reaction cell 31, for providing a reaction site for the whole blood sample and reagents to be tested, and the whole blood sample is discharged to the reaction cell 31.

The whole blood sample is treated with a reagent to obtain a sample to be tested. In some embodiments, the reagent may include any one or combination of a variety of reagents, such as a hemolysis reagent, a diluent, and a latex reagent.

In one possible embodiment of the present invention, when the detection part 30 detects a specific item, the whole blood sample and the reagent are conveyed to the reaction tank 31 through the liquid path support module to react to prepare a sample to be detected, the light emitting end of the optical device 32 emits light capable of irradiating the sample to be detected in the reaction tank, and the light detecting end of the optical device 32 receives the light passing through the sample to be detected in the reaction tank, so that the detection of the specific item is performed; the means for detecting the reaction of a whole blood sample and outputting the detection result is collectively referred to as a measurement channel. The measurement channel comprises at least: a reaction cell 31 and optics 32.

Referring to fig. 3, fig. 3 is a schematic structural diagram of a measurement channel; a measuring channel includes a reaction cell 31, a light emitting end 32-1, a light detecting end 32-2, a transporting portion 3-1, and a waste liquid discharging portion 3-2. The working state of the measuring channel is as follows: the reagent and the whole blood sample are added into a reaction tank 31 through a transportation part 3-1 to react under the drive of a liquid path support module 4 so as to prepare a sample to be tested; the light emitting end 32-1 emits light which can irradiate the sample to be measured in the reaction tank 31, and the light detecting end 32-2 receives the light which passes through the sample to be measured in the reaction tank 31 to detect a specific item; when the detection is completed or the waste liquid needs to be emptied, the waste liquid is discharged out of the reaction tank 31 by the waste liquid discharging part 3-2 under the drive of the liquid path support module 4.

It should be noted that the processor 40 in some embodiments of the present invention includes, but is not limited to, a processor (Central ProcessingUnit, CPU), a Micro control unit (Micro ControllerUnit, MCU), a Field programmable gate array (Field-Programmable Gate Array, FPGA), and a Digital Signal Processor (DSP) for analyzing computer instructions and processing data in computer software. In some embodiments, processor 40 is configured to execute computer applications in the non-transitory computer readable storage medium to cause the blood analysis device to perform a corresponding procedure and analyze the optical signals detected by processing optics 32 to obtain corresponding results.

In some embodiments of the present invention, when performing the detection of a particular item, the processor 40 controls the optical device 32 to acquire an optical signal of the test sample in the reaction cell to acquire a reaction curve of the whole blood sample and the reagent while controlling the reagent supplying part 20 to deliver the reagent to the reaction cell 31 to cause the whole blood sample and the reagent to relatively flow to prepare the test sample.

As can be seen from the above description of fig. 3, the reaction cell 31 is provided with an optical device 32, for example, a light emitting end 32-1 and a light detecting end 32-2 are respectively disposed at two sides of the reaction cell 31; therefore, in the process of mixing the whole blood sample and the reagent in the reaction cell 31 (mixing in a swirling manner), the processor 40 may obtain the optical signal of the sample to be tested in the reaction cell 31 by controlling the optical device 32, and obtain the reaction curve of the whole blood sample and the reagent according to the obtained optical signal. That is, the reaction profile of the whole blood sample and the reagent during the vortex mixing process is monitored throughout by the optics 32.

It should be noted that, as a result of repeated studies by the inventors, the reaction curve between the whole blood sample and the reagent in the reaction cell 31 when the liquid is normally fed (no mixing abnormality occurs) is characterized by: the reaction curve rises rapidly and the reaction curve stabilizes rapidly. When the liquid is filled abnormally or the pipeline is blocked (mixing abnormality occurs), the reaction curve of the whole blood sample and the reagent is characterized in that: the reaction curve rises slowly and takes a longer time to stabilize. Therefore, according to the characteristics of the reaction curve found by the above-mentioned studies, it is possible to determine whether or not the reaction cell is abnormal by determining the reaction curve between the whole blood sample obtained and the reagent.

In some embodiments, the specific way of obtaining the reaction curve of the whole blood sample and the reagent according to the optical signal of the test sample is: the processor 40 converts the optical signal into an electrical signal and determines a response curve based on the electrical signal. On this basis, the reaction curve of the above-mentioned reaction cell 31 at the time of normal liquid charging is characterized by: the voltage rises rapidly and tends to stabilize rapidly. The reaction curve of the reaction cell 31 mentioned above is characterized by: the voltage rises slowly and takes a long time to stabilize. That is, the horizontal axis of the response curve represents time and the vertical axis represents voltage. The rate of voltage rise can be determined in particular by the slope of the response curve.

In some embodiments, one way to determine whether the reaction tank 31 is abnormal is to: the processor 40 determines the length of time it takes for the reaction curve of the whole blood sample and the reagent to stabilize, i.e., the length of time it takes for the voltage in the reaction curve to rise to stabilize; wherein the response curve tends to stabilize (or the voltage tends to stabilize) such that the fluctuation range of the response curve (i.e., the fluctuation range of the voltage) is within a preset range of amplitude; if the time spent by the reaction curve tending to stabilize is longer than the preset time, namely the voltage of the reaction curve rises slowly and takes a longer time to stabilize, determining that the reaction tank has abnormal mixing; if the time spent by the reaction curve tending to stabilize is not longer than the preset time, namely the voltage of the reaction curve rapidly rises and rapidly tends to stabilize, determining that the reaction tank is not subjected to uniform mixing abnormality.

It is to be understood that, as a result of repeated researches by the inventor, the reaction curve of the reaction tank 31 may be characterized by: the reaction curve rises slowly and takes a longer time to stabilize, and the reaction curve has a larger fluctuation.

To further improve the accuracy of determining that the reaction tank 31 is abnormal in mixing, the fluctuation degree of the reaction curve can be determined by introducing a standard deviation, and then the abnormal mixing of the reaction tank 31 can be determined by combining the time spent for tending to stabilize and the fluctuation degree; specifically, the processor 40 determines the length of time it takes for the reaction curve to stabilize, and determines the standard deviation of the data points (i.e., voltages) on the reaction curve; wherein, the larger the standard deviation is, the larger the fluctuation of the reaction curve is; if the time spent by the reaction curve tending to stabilize is longer than the preset time and the determined standard deviation is greater than the standard deviation threshold, namely the voltage of the reaction curve rises slowly and takes a longer time to stabilize, and the reaction curve has larger fluctuation (here, the standard deviation is greater than the standard deviation threshold), the abnormal mixing of the reaction tank is determined. The method for determining that the reaction tank is not abnormal in mixing can be referred to above, and will not be described herein.

In some embodiments, another way to determine whether the reaction tank 31 is abnormal is to: the processor 40 determines whether the reaction curve has stabilized for a predetermined period of time; if the reaction curve does not tend to be stable within the preset time, determining that the reaction tank 31 is abnormal in uniform mixing; if the reaction curve tends to be stable within the preset time period, it is determined that the reaction tank 31 is not abnormal in mixing.

That is, if the reaction curve can be stabilized within the preset period of time, that is, the voltage of the reaction curve is rapidly increased and can be rapidly stabilized within the preset period of time, it is determined that the mixing abnormality does not occur in the reaction tank 31; if the reaction curve fails to stabilize within the preset time period, that is, the voltage of the reaction curve rises slowly and fails to stabilize rapidly within the preset time period, it is determined that the mixing abnormality occurs in the reaction tank 31.

In some embodiments, if it is determined that the reaction tank 31 is abnormal, an alarm message indicating that the reaction tank 31 is abnormal may be output, and a detection result of a specific item may be masked, and the material in the reaction tank 31 may be emptied; similarly, if it is determined that the mixing abnormality occurs in the reaction tank 31, other processing strategies may be employed, and the processing strategy for the mixing abnormality occurring in the reaction tank 31 is not particularly limited in the present invention.

The above embodiments are about the determination of whether or not the mixing abnormality occurs in the reaction cell 31 using the reaction curve of the whole blood sample and the reagent, in which various ways of determining the mixing abnormality of the reaction cell 31 are given; specifically, if the reaction curve rapidly rises and the reaction curve rapidly tends to stabilize, it is determined that no mixing abnormality occurs in the reaction tank 31; if the reaction curve rises slowly and takes a long time to stabilize, it is determined that the mixing abnormality occurs in the reaction tank 31. For example, an exemplary graph of a plurality of reaction curves shown in FIG. 4, comprising a total of 8 reaction curves of normal 1-normal 4 and abnormal 1-abnormal 4, each reaction curve corresponding to one specific item of detection (hemoglobin (HGB) detection in FIG. 4); wherein, the response curves corresponding to the normal 1 to the normal 4 are rapidly increased and the response curves rapidly tend to be stable, so that the reaction tank 31 is not uniformly mixed in the detection corresponding to the normal 1 to the normal 4; the reaction curves corresponding to anomalies 1 to 4 rise slowly and take a long time to stabilize, and the reaction curves corresponding to anomalies 1 to 4 have large fluctuation, so that in the detection corresponding to anomalies 1 to 4, the reaction tank 31 is uniformly mixed and abnormal.

It should be noted that the exemplary graph of the reaction curve shown in fig. 4 is only for explanation, and the content of the present solution is not specifically limited.

The method is related to how to determine whether the mixing abnormality occurs in the reaction tank, on the basis of utilizing the existing optical device (namely, without additionally adding a monitor component), whether the mixing abnormality occurs in the reaction tank is determined by detecting the reaction curve of the reaction tank in the cyclone mixing process, and when the mixing abnormality occurs in the reaction tank, a corresponding processing strategy is timely adopted to prevent risks and harm and avoid outputting inaccurate detection results.

The detection of the specific item at least comprises the detection of specific protein and the detection of hemoglobin; the reagents required for the detection of different specific items may be different, i.e. the reagents that are required to be delivered to the reaction cell 31 for the detection of different specific items may be different, as described in detail below.

In some embodiments, the detection of a particular item comprises detection of a particular protein, the reaction cell 31 comprises a first reaction cell, and the optics 32 comprises a first optics; when the specific protein detection is performed, the processor 40 controls the sampling part 10 to draw a first whole blood sample and transfer the first whole blood sample to the first reaction cell, and controls the reagent supplying part 20 to transfer the first hemolytic agent to the first reaction cell to prepare a first sample to be tested; when the first reagent supplying section 20 is controlled to deliver the first hemolytic agent to the first reaction tank so that the first whole blood sample and the first hemolytic agent relatively flow to prepare a first sample to be tested, the processor 40 controls the first optical device to obtain a first optical signal of the first sample to be tested in the first reaction tank so as to obtain a reaction curve of the first whole blood sample and the first hemolytic agent. That is, when performing a specific protein assay, the reaction profile obtained by the processor 40 is the reaction profile of the first whole blood sample and the first hemolyzing agent.

After the processor 40 obtains the reaction curve of the first whole blood sample and the first hemolysis agent, the processor 40 determines whether the first reaction tank is abnormal in mixing according to the obtained reaction curve; if the time spent by the reaction curve tending to stabilize is not longer than the preset time, determining that the first reaction tank is not abnormal in mixing; the specific way of determining whether the first reaction tank is abnormal in mixing may be referred to above, and details about how to determine whether the reaction tank 31 is abnormal in mixing will not be described herein.

In the case that it is determined that the first reaction tank is not abnormal in mixing, the processor 40 controls the reagent supply part 20 to deliver the latex reagent into the first reaction tank to react with the first sample to be tested to prepare a specific protein detection sample, and controls the first optical device to acquire a second optical signal of the specific protein detection sample in the first reaction tank; the concentration of the specific protein in the first whole blood sample is obtained from the second optical signal.

The specific proteins include one or more of C-reactive protein, serum amyloid, procalcitonin, interleukin-6, human chorionic gonadotropin, growth hormone, luteinizing hormone, alpha fetoprotein, and carcinoembryonic antigen.

In other embodiments, the detection of a particular item comprises hemoglobin detection, the reaction cell 31 comprises a second reaction cell, and the optics 32 comprises a second optics; when the hemoglobin measurement is performed, the processor 40 controls the sampling part 10 to draw a second whole blood sample and transfer the second whole blood sample to the second reaction cell, and controls the reagent supply part 20 to transfer the diluent to the second reaction cell to prepare a second sample to be measured; when the reagent supplying section 20 is controlled to deliver the diluent to the second reaction tank to enable the second whole blood sample and the diluent to flow relatively to prepare the second to-be-measured sample, the processor 40 controls the second optical device to obtain the second optical signal of the second to-be-measured sample in the second reaction tank to obtain the reaction curve of the second whole blood sample and the diluent. That is, when performing hemoglobin measurement, the reaction curve obtained by the processor 40 is the reaction curve of the second whole blood sample and the diluent.

After the processor 40 obtains the reaction curve of the second whole blood sample and the diluent, the processor 40 determines whether the second reaction tank is abnormal in mixing according to the obtained reaction curve; if the time spent by the reaction curve tending to stabilize is not longer than the preset time, determining that the second reaction tank is not abnormal in mixing; the specific way of determining whether the second reaction tank is abnormal in mixing may be referred to above, and details about how to determine whether the reaction tank 31 is abnormal in mixing will not be described herein.

In the case where it is determined that the second reaction tank is not abnormal in mixing, the processor 40 controls the reagent supply section 20 to supply the second hemolytic agent into the second reaction tank to react with the second sample to be measured to prepare a hemoglobin detection sample, and controls the second optical device to acquire a third optical signal of the hemoglobin detection sample in the second reaction tank to obtain the hemoglobin concentration in the second whole blood sample, that is, to obtain the hemoglobin concentration in the second whole blood sample from the third optical signal.

As can be seen from the above, the detection of a specific item includes the detection of a specific protein, the reaction cell 31 includes a first reaction cell, and the optical device 32 includes a first optical device; the detection of a specific item includes hemoglobin detection, the reaction cell 31 includes a second reaction cell, and the optical device 32 includes a second optical device. It should be noted that the first reaction tank and the second reaction tank may be the same reaction tank, and the first optical device and the second optical device may be the same optical device, i.e. different specific items may share one reaction tank and one optical device; similarly, the first reaction tank and the second reaction tank may be different reaction tanks, and the first optical device and the second optical device may be different optical devices, i.e., different specific projects may use different reaction tanks and different optical devices.

The first hemolyzing agent used for detecting a specific protein and the second hemolyzing agent used for detecting hemoglobin may be different types of hemolyzing agents or the same type of hemolyzing agents.

The method is related to how to determine whether the mixing abnormality occurs in the reaction tank in the detection of different specific items, and by detecting the reaction curve of the reaction tank in the cyclone mixing process and determining whether the mixing abnormality occurs in the reaction tank according to the reaction curve, a corresponding treatment strategy is adopted in time to prevent risks and harm from occurring when the mixing abnormality occurs in the reaction tank, and inaccurate detection results are avoided to be output.

The foregoing is a description of a blood analysis device, and in some embodiments of the invention, blood analysis methods are disclosed.

Referring to fig. 5, the blood analysis method in some embodiments includes the following steps:

step S501: when reagent is conveyed to the reaction tank so that the whole blood sample and the reagent relatively flow to prepare a to-be-tested sample, the optical signal of the to-be-tested sample in the reaction tank is obtained to obtain the reaction curve of the whole blood sample and the reagent.

In the specific implementation step S501, when the reagent is delivered to the reaction tank to enable the whole blood sample and the reagent to flow relatively to prepare a sample to be tested during the detection of a specific item, acquiring an optical signal of the sample to be tested in the reaction tank; the acquired optical signal is converted into an electrical signal and a response curve is determined based on the electrical signal.

Step S502: the length of time it takes for the reaction curve to stabilize is determined.

It should be noted that the response curve tends to stabilize such that the fluctuation range of the response curve is within a preset range of amplitude.

Step S503: if the time spent by the reaction curve tending to stabilize is longer than the preset time, determining that the reaction tank is abnormal in mixing.

In some embodiments, the length of time it takes for the reaction curve to stabilize is determined, as well as the standard deviation of each data point on the reaction curve; if the time spent by the reaction curve tending to stabilize is longer than the preset time and the standard deviation is greater than the standard deviation threshold, determining that the reaction tank is abnormal in mixing.

In some embodiments, the detection of the particular item comprises detection of a particular protein, and the reaction cell comprises a first reaction cell; when specific protein detection is carried out, a first whole blood sample is extracted, the first whole blood sample is conveyed to a first reaction tank, and a first hemolysis agent is conveyed to the first reaction tank to prepare a first sample to be tested; when the first hemolytic agent is conveyed to the first reaction tank so that the first whole blood sample and the first hemolytic agent relatively flow to prepare a first to-be-tested sample, a first optical signal of the first to-be-tested sample in the first reaction tank is obtained so as to obtain a reaction curve of the first whole blood sample and the first hemolytic agent.

If the time length spent by the reaction curve tending to stabilize is not longer than the preset time length, determining that the first reaction tank is not abnormal in mixing; under the condition that the first reaction tank is not abnormal in mixing, conveying the latex reagent into the first reaction tank to react with a first sample to be tested to prepare a specific protein detection sample, and acquiring a second optical signal of the specific protein detection sample in the first reaction tank; the concentration of the specific protein in the first whole blood sample is obtained from the second optical signal.

In other embodiments, the detection of the particular item comprises a hemoglobin detection, and the reaction cell comprises a second reaction cell; when hemoglobin detection is performed, a second whole blood sample is drawn and conveyed to a second reaction tank, and diluent is conveyed to the second reaction tank to prepare a second to-be-detected sample; when the reagent supply part is controlled to convey the diluent to the second reaction tank so that the second whole blood sample and the diluent relatively flow to prepare a second to-be-tested sample, a second optical signal of the second to-be-tested sample in the second reaction tank is obtained to obtain a reaction curve of the second whole blood sample and the diluent.

If the time length spent by the reaction curve tending to stabilize is not longer than the preset time length, determining that the second reaction tank is not abnormal in mixing; and under the condition that the second reaction tank is not abnormal in mixing, conveying the second hemolytic agent into the second reaction tank to react with the second sample to be detected to prepare a hemoglobin detection sample, and acquiring a third optical signal of the hemoglobin detection sample in the second reaction tank to obtain the concentration of hemoglobin in the second whole blood sample.

Referring to fig. 6, in other embodiments, the blood analysis method includes the following steps:

step S601: when reagent is conveyed to the reaction tank so that the whole blood sample and the reagent relatively flow to prepare a to-be-tested sample, the optical signal of the to-be-tested sample in the reaction tank is acquired to acquire the reaction curve of the whole blood sample and the reagent.

Step S602: it is determined whether the reaction curve has tended to stabilize for a predetermined period of time.

It should be noted that the response curve tends to stabilize such that the fluctuation range of the response curve is within a preset range of amplitude.

Step S603: if the reaction curve does not tend to be stable within the preset time, determining that the reaction tank is abnormal in uniform mixing.

The principle of performing each step in fig. 5 and 6 is described in detail in the above description of the blood analysis device, and will not be described in detail here.

From the above, it can be seen that, after the present invention is applied, an application scenario may be: when the detection of the specific item is carried out, a reagent is conveyed to the reaction tank so that the whole blood sample and the reagent relatively flow to prepare a to-be-detected sample, an optical signal of the to-be-detected sample in the reaction tank is obtained, and a reaction curve of the whole blood sample and the reagent is obtained according to the obtained optical signal. And determining whether the reaction tank is abnormal in mixing or not by using a reaction curve. When the abnormal mixing of the reaction tank is determined, processing strategies such as alarm information output and detection result shielding are adopted, so that risks and harm can be prevented, and inaccurate detection results can be prevented from being output.

It should be noted that the terms "first," "second," and the like herein are merely used for distinguishing between different devices, modules, or units and not for limiting the order or interdependence of the functions performed by such devices, modules, or units.

Those skilled in the art can make or use the present application. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the application. Thus, the present application is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims (10)

1. A blood analysis device, comprising: a sampling unit, a reagent supply unit, a detection unit, and a processor;

the sampling part is used for drawing a whole blood sample and conveying the whole blood sample to the detection part;

the reagent supply part is used for conveying the reagent to the detection part;

the detection part comprises a reaction tank and an optical device, wherein the reaction tank is used for receiving the whole blood sample conveyed by the sampling part and the reagent conveyed by the reagent supply part to prepare a sample to be detected; the optical device comprises a light emitting end and a light detecting end, wherein the light emitting end is used for emitting light capable of irradiating the to-be-detected sample in the reaction tank, and the light detecting end is used for receiving the light passing through the to-be-detected sample in the reaction tank so as to detect specific items;

The processor, wherein:

the processor is further configured to control the optical device to acquire an optical signal of the sample to be measured in the reaction cell to acquire a reaction curve of the whole blood sample and the reagent when controlling the reagent supply section to deliver the reagent to the reaction cell to cause the whole blood sample and the reagent to relatively flow to prepare the sample to be measured when the detection of the specific item is performed;

the processor determines the time length spent by the reaction curve tending to stabilize, and determines that the reaction tank is abnormal in mixing if the time length spent by the reaction curve tending to stabilize is longer than a preset time length; wherein the response curve tends to stabilize such that the fluctuation amplitude of the response curve is within a preset amplitude range.

2. The blood analysis device of claim 1, wherein the processor is specifically configured to: determining the time period taken for the reaction curve to stabilize and determining the standard deviation of each data point on the reaction curve;

if the time spent by the reaction curve tending to stabilize is longer than the preset time and the standard deviation is greater than the standard deviation threshold, determining that the reaction tank is abnormal in mixing.

3. The blood analysis device of claim 1, wherein the processor is specifically configured to: the optical signal is converted to an electrical signal and the response curve is determined based on the electrical signal.

4. A blood analysis device according to claims 1-3, wherein the detection of the specific item comprises a specific protein detection, the reaction cell comprises a first reaction cell, the optical device comprises a first optical device, the processor is specifically configured to:

when specific protein detection is performed, the sampling part is controlled to draw a first whole blood sample and convey the first whole blood sample to the first reaction tank, and the reagent supply part is controlled to convey a first hemolytic agent to the first reaction tank so as to prepare a first sample to be tested, wherein the processor is further used for controlling the first optical device to acquire a first optical signal of the first sample to be tested in the first reaction tank so as to acquire a reaction curve of the first whole blood sample and the first hemolytic agent when the reagent supply part is controlled to convey the first hemolytic agent to the first reaction tank so as to enable the first whole blood sample and the first hemolytic agent to relatively flow to prepare the first sample to be tested.

5. The blood analysis device of claim 4, wherein the processor is further configured to:

if the time spent by the reaction curve tending to stabilize is not longer than the preset time, determining that the first reaction tank is not abnormal in mixing;

controlling the reagent supply part to convey a latex reagent into the first reaction tank to react with the first sample to be tested to prepare a specific protein detection sample under the condition that the first reaction tank is determined not to have abnormal mixing, and controlling the first optical device to acquire a second optical signal of the specific protein detection sample in the first reaction tank; and obtaining the concentration of the specific protein in the first whole blood sample according to the second optical signal.

6. The blood analysis device of any one of claims 1-5, wherein the detection of the particular item includes hemoglobin detection, the reaction cell includes a second reaction cell, the optics includes a second optics, and the processor is configured to:

when hemoglobin detection is performed, the sampling part is controlled to draw a second whole blood sample and convey the second whole blood sample to the second reaction tank, and the reagent supply part is controlled to convey a diluent to the second reaction tank to prepare a second test sample, wherein the processor is further used for controlling the second optical device to acquire a second optical signal of the second test sample in the second reaction tank to acquire a reaction curve of the second whole blood sample and the diluent when the reagent supply part is controlled to convey the diluent to the second reaction tank to enable the second whole blood sample and the diluent to flow relatively to prepare the second test sample.

7. The blood analysis device of claim 6, wherein the processor is further configured to:

if the time spent by the reaction curve tending to stabilize is not longer than the preset time, determining that the second reaction tank is not abnormal in mixing;

and under the condition that the second reaction tank is not subjected to uniform mixing abnormality, controlling the reagent supply part to convey a second hemolytic agent into the second reaction tank to react with the second to-be-detected sample so as to prepare a hemoglobin detection sample, and controlling the second optical device to acquire a third optical signal of the hemoglobin detection sample in the second reaction tank so as to acquire the hemoglobin concentration in the second whole blood sample.

8. A blood analysis device, comprising: a sampling unit, a reagent supply unit, a detection unit, and a processor;

the sampling part is used for drawing a whole blood sample and conveying the whole blood sample to the detection part;

the reagent supply part is used for conveying the reagent to the detection part;

the detection part comprises a reaction tank and an optical device, wherein the reaction tank is used for receiving the whole blood sample conveyed by the sampling part and the reagent conveyed by the reagent supply part to prepare a sample to be detected; the optical device comprises a light emitting end and a light detecting end, wherein the light emitting end is used for emitting light capable of irradiating the to-be-detected sample in the reaction tank, and the light detecting end is used for receiving the light passing through the to-be-detected sample in the reaction tank so as to detect specific items;

The processor, wherein:

the processor is further configured to control the optical device to acquire an optical signal of the sample to be measured in the reaction cell to acquire a reaction curve of the whole blood sample and the reagent when controlling the reagent supply section to deliver the reagent to the reaction cell to cause the whole blood sample and the reagent to relatively flow to prepare the sample to be measured when the detection of the specific item is performed;

the processor determines whether the reaction curve tends to be stable within a preset time period; if the reaction curve does not tend to be stable within the preset time, determining that the reaction tank is abnormal in uniform mixing; wherein the response curve tends to stabilize such that the fluctuation amplitude of the response curve is within a preset amplitude range.

9. A method of blood analysis, the method comprising:

when a reagent is conveyed to a reaction tank so that a whole blood sample and the reagent relatively flow to prepare a to-be-tested sample, acquiring an optical signal of the to-be-tested sample in the reaction tank so as to acquire a reaction curve of the whole blood sample and the reagent;

determining the length of time it takes for the reaction curve to stabilize;

if the time spent by the reaction curve tending to stabilize is longer than a preset time, determining that the reaction tank is abnormal in uniform mixing;

Wherein the response curve tends to stabilize such that the fluctuation amplitude of the response curve is within a preset amplitude range.

10. A method of blood analysis, the method comprising:

when a reagent is conveyed to a reaction tank so that a whole blood sample and the reagent relatively flow to prepare a to-be-tested sample, acquiring an optical signal of the to-be-tested sample in the reaction tank so as to acquire a reaction curve of the whole blood sample and the reagent;

determining whether the reaction curve tends to be stable within a preset time period;

if the reaction curve does not tend to be stable within the preset time, determining that the reaction tank is abnormal in uniform mixing;

wherein the response curve tends to stabilize such that the fluctuation amplitude of the response curve is within a preset amplitude range.

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