CN116284502A - Preparation method of rye bran source feruloyl arabinoxylans - Google Patents

Preparation method of rye bran source feruloyl arabinoxylans Download PDF

Info

Publication number
CN116284502A
CN116284502A CN202310160401.2A CN202310160401A CN116284502A CN 116284502 A CN116284502 A CN 116284502A CN 202310160401 A CN202310160401 A CN 202310160401A CN 116284502 A CN116284502 A CN 116284502A
Authority
CN
China
Prior art keywords
feruloyl
arabinoxylans
bran
ultrasonic
rye bran
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202310160401.2A
Other languages
Chinese (zh)
Inventor
陈曦
李依云
吕庆云
丁文平
王月慧
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Wuhan Polytechnic University
Original Assignee
Wuhan Polytechnic University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wuhan Polytechnic University filed Critical Wuhan Polytechnic University
Priority to CN202310160401.2A priority Critical patent/CN116284502A/en
Publication of CN116284502A publication Critical patent/CN116284502A/en
Pending legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
    • C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
    • C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
    • C08B37/0057—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid beta-D-Xylans, i.e. xylosaccharide, e.g. arabinoxylan, arabinofuronan, pentosans; (beta-1,3)(beta-1,4)-D-Xylans, e.g. rhodymenans; Hemicellulose; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87—Re-use of by-products of food processing for fodder production

Abstract

The invention discloses a preparation method of rye bran-derived feruloyl arabinoxylans, which comprises the following steps of: pretreating rye bran raw materials, and removing proteins and starch in the wheat bran to obtain pretreated wheat bran; mixing the pretreated wheat bran with xylanase, and performing ultrasonic auxiliary extraction to obtain an extract; and (3) separating and purifying the extract to obtain the black wheat bran source feruloyl arabinoxylan. The method adopts the ultrasonic-assisted extraction combined with the enzymatic method to extract the arabinoxylans in the wheat bran, is milder compared with the common alkaline method, has better retention effect on the ferulic acid, can effectively reduce the loss of the ferulic acid in the extraction process, and improves the content of the ferulic acid in the feruloyl arabinoxylans obtained by extraction, thereby improving the oxidation resistance and prebiotic effect of the feruloyl arabinoxylans.

Description

Preparation method of rye bran source feruloyl arabinoxylans
Technical Field
The invention relates to the technical field of agricultural and sideline product processing, in particular to a preparation method of rye bran source feruloyl arabinoxylans.
Background
The method for preparing the wheat bran-derived feruloyl arabinoxylans is widely used at present, for example, alkali extraction is adopted, alkali is used for extraction, however, the alkali is used for extracting the arabinoxylans from the wheat bran, so that ferulic acid on the arabinoxylans falls off in the extraction process, the ferulic acid content of the extracted arabinoxylans is low, even no ferulic acid is contained, and a series of activities such as prebiotic effect, oxidation resistance and the like of the arabinoxylans are reduced.
Disclosure of Invention
The invention mainly aims to provide a preparation method of rye bran-derived feruloyl arabinoxylans, aiming at improving the content of ferulic acid in the feruloyl arabinoxylans obtained by extraction.
In order to achieve the aim, the invention provides a preparation method of rye bran-derived feruloyl arabinoxylans, which comprises the following steps:
pretreating rye bran raw materials to remove proteins and starch in the wheat bran, so as to obtain pretreated wheat bran;
mixing the pretreated wheat bran with xylanase, and performing ultrasonic auxiliary extraction to obtain an extract;
and (3) separating and purifying the extract to obtain the black wheat bran source feruloyl arabinoxylan.
Optionally, the mass ratio of the xylanase to the rye bran raw material is 1:120-130.
Optionally, the mass ratio of xylanase to rye bran feedstock is 1:125.
Optionally, the ultrasonic temperature of the ultrasonic assisted extraction is 54-58 ℃, the ultrasonic power is 150-170W, the ultrasonic frequency is 45-55 kHz, and the ultrasonic extraction time is 80-100 min.
Optionally, the ultrasonic temperature of the ultrasonic assisted extraction is 56 ℃, the ultrasonic power is 160W, the ultrasonic frequency is 50kHz, and the ultrasonic extraction time is 90min.
Optionally, the extraction solvent for ultrasonic-assisted extraction is deionized water, and 18-22L of deionized water is correspondingly added into each 1kg of pretreated wheat bran.
Optionally, the rye bran material comprises Jizi 439 black wheat bran.
Optionally, the rye bran raw material is black wheat bran powder with the particle size of 30-50 meshes.
Optionally, the step of pre-treating the rye bran feedstock comprises:
neutral protease was used to remove proteins and alpha-amylase was used to remove starch.
Optionally, the step of separating and purifying the extract to obtain the black wheat bran-derived feruloyl arabinoxylans comprises the steps of:
centrifuging the extract, collecting supernatant, dialyzing, concentrating, and freeze drying to obtain the final product.
According to the technical scheme provided by the invention, the feruloyl arabinoxylans in the black wheat bran are extracted by adopting an ultrasonic-assisted extraction combined enzyme method, so that the method is milder compared with a common alkaline method, has a better retention effect on the ferulic acid, can effectively reduce the loss of the ferulic acid in the extraction process, and improves the content of the ferulic acid in the extracted arabinoxylans, thereby improving the oxidation resistance and prebiotic effect of the arabinoxylans.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are required in the embodiments or the description of the prior art will be briefly described, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and other related drawings can be obtained according to the drawings without inventive effort for a person skilled in the art.
Fig. 1 is a schematic flow chart of an embodiment of a method for preparing rye bran-derived feruloyl arabinoxylans.
The achievement of the objects, functional features and advantages of the present invention will be further described with reference to the accompanying drawings, in conjunction with the embodiments.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention more clear, the technical solutions of the embodiments of the present invention will be clearly and completely described below. The specific conditions are not noted in the examples and are carried out according to conventional conditions or conditions recommended by the manufacturer. The reagents or apparatus used were conventional products commercially available without the manufacturer's attention. In addition, the meaning of "and/or" as it appears throughout includes three parallel schemes, for example "A and/or B", including the A scheme, or the B scheme, or the scheme where A and B are satisfied simultaneously. In addition, the technical solutions of the embodiments may be combined with each other, but it is necessary to base that the technical solutions can be realized by those skilled in the art, and when the technical solutions are contradictory or cannot be realized, the combination of the technical solutions should be regarded as not exist and not within the protection scope of the present invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
The method for preparing the wheat bran-derived feruloyl arabinoxylans is widely used at present, for example, alkali extraction is adopted, alkali is used for extraction, however, the alkali is used for extracting the arabinoxylans from the wheat bran, so that ferulic acid on the arabinoxylans falls off in the extraction process, the ferulic acid content of the extracted arabinoxylans is low, even no ferulic acid is contained, and a series of activities such as prebiotic effect, oxidation resistance and the like of the arabinoxylans are reduced. The inventor finds that the ferulic acid content in rye bran is higher than that in wheat bran, based on which the invention provides a preparation method of rye bran source feruloyl arabinoxylans, and fig. 1 shows an embodiment of the preparation method of rye bran source feruloyl arabinoxylans.
Referring to fig. 1, in this embodiment, the preparation method of the rye bran source feruloyl arabinoxylans comprises the following steps:
step S10, preprocessing rye bran raw materials to remove proteins and starch in the wheat bran, so as to obtain preprocessed wheat bran;
step S20, mixing the pretreated wheat bran with xylanase, and performing ultrasonic-assisted extraction to obtain an extract;
and step S30, separating and purifying the extract to obtain the black wheat bran source feruloyl arabinoxylans.
Firstly, preprocessing a rye bran raw material, and removing proteins and starch from the rye bran raw material to obtain preprocessed wheat bran; mixing the extract with xylanase, and extracting by adopting an enzymatic method and an ultrasonic auxiliary extraction mode to obtain an extract of feruloyl arabinoxylan; finally, the feruloyl arabinoxylans in the process of extracting are separated and purified, and the rye bran source feruloyl arabinoxylans are obtained.
In the technical scheme provided by the invention, the feruloyl arabinoxylans in the black wheat bran are extracted by adopting an ultrasonic-assisted extraction combined enzyme method, so that the method is milder compared with a common alkaline method, has a better retention effect on the ferulic acid, can effectively reduce the loss of the ferulic acid in the extraction process, and improves the content of the ferulic acid in the extracted feruloyl arabinoxylans, thereby improving the free radical scavenging property and prebiotic effect of the arabinoxylans.
In some embodiments of the invention, the mass ratio of xylanase to rye bran feedstock is 1:120-130. In some embodiments of the invention, the mass ratio of the xylanase to the rye bran material is 1:125.
In some embodiments of the invention, the ultrasonic temperature of the ultrasonic-assisted extraction is 54-58 ℃, the ultrasonic power is 150-170W, the ultrasonic frequency is 45-55 kHz, and the ultrasonic extraction time is 80-100 min. In some embodiments of the invention, the ultrasound temperature of the ultrasound-assisted extraction is 56 ℃, the ultrasound power is 160W, the ultrasound frequency is 50kHz, and the ultrasound extraction time is 90min.
In some embodiments of the present invention, the extraction solvent for the ultrasonic-assisted extraction is deionized water, and 18-22L of deionized water is added to each 1kg of the pretreated wheat bran for ultrasonic-assisted extraction. In some embodiments of the invention, 20L deionized water is added for each 1kg of the pretreated wheat bran.
The method for extracting feruloyl arabinoxylans from the black wheat bran is basically applicable to all types of wheat at present, preferably winter wheat, and more preferably Ji Zi 439 rye. That is, in some embodiments of the invention, the rye bran material includes Ji Zi 439 rye bran.
In addition, in some embodiments of the invention, the rye bran material is wheat bran powder having a particle size of 30-50 mesh. The powdered wheat bran powder is used as a raw material, so that the preparation efficiency of the subsequent steps is improved, for example, the treatment efficiency of removing protein and starch is improved, the extraction efficiency of feruloyl arabinoxylan is improved, and the like. Specifically, wheat bran can be firstly ground and then sieved by a sieve with 30-50 meshes, and the wheat bran powder is obtained.
In some embodiments of the invention, the step of pre-treating the rye bran feedstock in step S10 comprises: neutral protease was used to remove proteins and alpha-amylase was used to remove starch.
The method for separating feruloyl arabinoxylans in the extract comprises initially separating solid substances in the extract by centrifugation, filtration, etc., further refining by dialysis and concentration, and drying to remove water. Specifically, in some embodiments of the present invention, the step S30 step includes: centrifuging the extract, collecting supernatant, dialyzing, concentrating, and freeze drying to obtain the final product.
The following technical solutions of the present invention will be described in further detail with reference to specific examples and drawings, and it should be understood that the following examples are only for explaining the present invention and are not intended to limit the present invention.
Example 1
(1) Grinding Jizi 439 rye bran, sieving with 40 mesh sieve to obtain wheat bran powder, removing protein with neutral proteinase, and removing starch with alpha-amylase to obtain pretreated wheat bran.
(2) According to the mass of xylanase: mixing pretreated wheat bran and xylanase in a mass ratio of wheat bran powder=1:125, and then using deionized water as a solvent (20L of water is correspondingly added into each 1kg of pretreated wheat bran), and performing ultrasonic-assisted extraction by using an ultrasonic extractor to obtain an extract, wherein parameters of the ultrasonic-assisted extraction are set as follows: the ultrasonic temperature is 56 ℃, the ultrasonic power is 160W, the ultrasonic frequency is 50kHz, and the ultrasonic extraction time is 90min.
(3) Centrifuging the obtained extract, collecting supernatant, dialyzing the supernatant, concentrating, and lyophilizing to obtain the final product.
Through detection, the content of ferulic acid in the feruloyl arabinoxylans of the black wheat bran source obtained through extraction is 13.26 mug/mg.
Example 2
(1) Grinding Jizi 439 rye bran, sieving with 30 mesh sieve to obtain wheat bran powder, removing protein with neutral proteinase, and removing starch with alpha-amylase to obtain pretreated wheat bran.
(2) According to the mass of xylanase: mixing pretreated wheat bran and xylanase according to the mass ratio of wheat bran powder=1:120, and then using deionized water as a solvent (18L of water is correspondingly added into each 1kg of pretreated wheat bran), and performing ultrasonic-assisted extraction by using an ultrasonic extractor to obtain an extract, wherein parameters of the ultrasonic-assisted extraction are set as follows: the ultrasonic temperature is 54 ℃, the ultrasonic power is 150W, the ultrasonic frequency is 45kHz, and the ultrasonic extraction time is 80min.
(3) Centrifuging the obtained extract, collecting supernatant, dialyzing the supernatant, concentrating, and lyophilizing to obtain the final product.
Through detection, the content of ferulic acid in the extracted black wheat bran source feruloyl arabinoxylans is 11.96 mug/mg.
Example 3
(1) Grinding Jizi 439 rye bran, sieving with 35 mesh sieve to obtain wheat bran powder, removing protein with neutral protease, and removing starch with alpha-amylase to obtain pretreated wheat bran.
(2) According to the mass of xylanase: mixing pretreated wheat bran and xylanase according to the mass ratio of wheat bran powder=1:122, and then using deionized water as a solvent (22L of water is correspondingly added into each 1kg of pretreated wheat bran), and performing ultrasonic-assisted extraction by using an ultrasonic extractor to obtain an extract, wherein parameters of ultrasonic-assisted extraction are set as follows: the ultrasonic temperature is 55 ℃, the ultrasonic power is 155W, the ultrasonic frequency is 47kHz, and the ultrasonic extraction time is 85min.
(3) Centrifuging the obtained extract, collecting supernatant, dialyzing the supernatant, concentrating, and lyophilizing to obtain the final product.
Through detection, the content of ferulic acid in the extracted black wheat bran source feruloyl arabinoxylans is 12.64 mug/mg.
Example 4
(1) Grinding Jizi 439 rye bran, sieving with 45 mesh sieve to obtain wheat bran powder, removing protein with neutral proteinase, and removing starch with alpha-amylase to obtain pretreated wheat bran.
(2) According to the mass of xylanase: mixing pretreated wheat bran and xylanase according to the mass ratio of wheat bran powder=1:128, and then using deionized water as a solvent (20L of water is correspondingly added into each 1kg of pretreated wheat bran), and performing ultrasonic-assisted extraction by using an ultrasonic extractor to obtain an extract, wherein parameters of the ultrasonic-assisted extraction are set as follows: the ultrasonic temperature is 57 ℃, the ultrasonic power is 165W, the ultrasonic frequency is 53kHz, and the ultrasonic extraction time is 95min.
(3) Centrifuging the obtained extract, collecting supernatant, dialyzing the supernatant, concentrating, and lyophilizing to obtain the final product.
Through detection, the content of ferulic acid in the feruloyl arabinoxylans of the black wheat bran source obtained through extraction is 13.08 mug/mg.
Example 5
(1) Grinding Jizi 439 rye bran, sieving with 50 mesh sieve to obtain wheat bran powder, removing protein with neutral proteinase, and removing starch with alpha-amylase to obtain pretreated wheat bran.
(2) According to the mass of xylanase: mixing pretreated wheat bran and xylanase according to the mass ratio of wheat bran powder=1:130, and then using deionized water as a solvent (20L of water is correspondingly added into each 1kg of pretreated wheat bran), and performing ultrasonic-assisted extraction by using an ultrasonic extractor to obtain an extract, wherein parameters of the ultrasonic-assisted extraction are set as follows: the ultrasonic temperature is 58 ℃, the ultrasonic power is 170W, the ultrasonic frequency is 55kHz, and the ultrasonic extraction time is 100min.
(3) Centrifuging the obtained extract, collecting supernatant, dialyzing the supernatant, concentrating, and lyophilizing to obtain the final product.
Through detection, the content of ferulic acid in the extracted black wheat bran source feruloyl arabinoxylans is 12.15 mug/mg.
Comparative example 1
The extraction method of wheat bran-derived arabinoxylans was performed with reference to prior art 1 (Wang L, zhang L, qiau S, et al Rheologic properties and structural characteristics of arabinoxylan hydrogels prepared from three wheat bran sources [ J ]. Journal of cereal science,2019, 88:79-86).
The content of ferulic acid in the extracted wheat bran-derived arabinoxylan is 7.39 mug/mg.
Comparative example 2
The extraction method of wheat bran-derived arabinoxylans is carried out by referring to prior art 2 (Skendi A, biliadeis C G, izydorczyk M S, et al Structure variation and rheological properties of water-extractable arabinoxylans from six Greek wheat cultivars [ J ]. Food Chemistry,2011,126 (2): 526-536).
The content of ferulic acid in the extracted wheat bran arabinoxylans is 0.95 mug/mg.
The foregoing is merely a preferred embodiment of the present invention and is not intended to limit the scope of the present invention, but various modifications and variations will be apparent to those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention should be included in the scope of the present invention.

Claims (10)

1. A method for preparing rye bran-derived feruloyl arabinoxylans, which is characterized by comprising the following steps:
pretreating rye bran raw materials to remove proteins and starch in the wheat bran, so as to obtain pretreated wheat bran;
mixing the pretreated wheat bran with xylanase, and performing ultrasonic auxiliary extraction to obtain an extract;
and (3) separating and purifying the extract to obtain the black wheat bran source feruloyl arabinoxylan.
2. The method for preparing rye bran-derived feruloyl arabinoxylans according to claim 1, wherein the mass ratio of xylanase to rye bran raw material is 1:120-130.
3. The method for preparing rye bran-derived feruloyl arabinoxylans according to claim 2, wherein the mass ratio of xylanase to rye bran raw material is 1:125.
4. The method for preparing rye bran-derived feruloyl arabinoxylans according to claim 1, wherein the ultrasonic temperature of the ultrasonic-assisted extraction is 54-58 ℃, the ultrasonic power is 150-170W, the ultrasonic frequency is 45-55 kHz, and the ultrasonic extraction time is 80-100 min.
5. The method for preparing rye bran-derived feruloyl arabinoxylans according to claim 4, wherein the ultrasonic assisted extraction has an ultrasonic temperature of 56 ℃, an ultrasonic power of 160W, an ultrasonic frequency of 50kHz, and an ultrasonic extraction time of 90min.
6. The method for preparing rye bran-derived feruloyl arabinoxylans according to claim 1, wherein the extraction solvent for ultrasonic-assisted extraction is deionized water, and 18-22L of deionized water is added per 1kg of the pretreated wheat bran.
7. The method for preparing rye bran-derived feruloyl arabinoxylans according to claim 1 wherein the rye bran material comprises Ji Zi 439 rye bran.
8. The method for preparing rye bran-derived feruloyl arabinoxylans according to claim 1, wherein the rye bran raw material is rye bran powder with a particle size of 30-50 mesh.
9. The method for preparing rye bran-derived feruloyl arabinoxylans of claim 1 wherein the step of pre-treating the rye bran material comprises:
neutral protease was used to remove proteins and alpha-amylase was used to remove starch.
10. The method for preparing rye bran-derived feruloyl arabinoxylans according to claim 1, wherein the step of separating and purifying the extract to obtain the rye bran-derived feruloyl arabinoxylans comprises:
centrifuging the extract, collecting supernatant, dialyzing, concentrating, and freeze drying to obtain the final product.
CN202310160401.2A 2023-02-23 2023-02-23 Preparation method of rye bran source feruloyl arabinoxylans Pending CN116284502A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202310160401.2A CN116284502A (en) 2023-02-23 2023-02-23 Preparation method of rye bran source feruloyl arabinoxylans

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202310160401.2A CN116284502A (en) 2023-02-23 2023-02-23 Preparation method of rye bran source feruloyl arabinoxylans

Publications (1)

Publication Number Publication Date
CN116284502A true CN116284502A (en) 2023-06-23

Family

ID=86791676

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202310160401.2A Pending CN116284502A (en) 2023-02-23 2023-02-23 Preparation method of rye bran source feruloyl arabinoxylans

Country Status (1)

Country Link
CN (1) CN116284502A (en)

Similar Documents

Publication Publication Date Title
CN108703989B (en) Method for industrially preparing white kidney bean alpha-amylase inhibitor
JP4383048B2 (en) Grain separation methods and products
US20060029715A1 (en) Corn oil and dextrose extraction apparatus and method
CN101120776B (en) Method for extracting beta-glucan from cereal bran using membrane separation technology
CN111019011B (en) Method for extracting rice bran polysaccharide
CN104193833A (en) Process for screening and refining wheat starch
CN100535110C (en) Novel extraction method of bromelain
CN101314621A (en) Squash polysaccharide and preparation method thereof
CN114105747B (en) Method for improving curcumin extraction rate and purification effect
CN106754834A (en) A kind of preparation technology of high activity papain
CN111150069A (en) Method for preparing asparagus byproduct dietary fiber by adopting enzymolysis method and spray drying
CN101797036B (en) Preparation method of canna edulis ker soluble dietary fiber
CN116284502A (en) Preparation method of rye bran source feruloyl arabinoxylans
US6602985B1 (en) Extraction of zein protein from gluten meal
CN111035018A (en) Comprehensive utilization method of rice bran
CN113880942A (en) Chicken bone collagen peptide with antioxidant activity and application thereof
JPH02991B2 (en)
CN113481259A (en) Method for extracting starch syrup and rice protein isolate from broken rice
JP2005270766A (en) Method for extracting persimmon tannin and persimmon tannin extracted by the method
CN107467639A (en) A kind of coproduction prepares oat beta glucan nutrient powder and the method for dietary fiber micro powder
CN110522774A (en) The extraction separation method of Akebia trifoliate koiaz Peels polyphenol
CN102115736B (en) Purification method of plant chlorophyllase
CN110894518A (en) Preparation method of hericium erinaceus small peptide
CN101570743A (en) Earthworm composite multienzyme and preparation method thereof
JPS6157520A (en) Solution having high fucoidan purity, or preparation of fucoidan

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination