CN116284502A - A kind of preparation method of rye bran source feruloyl arabinoxylan - Google Patents

Abstract

The invention discloses a preparation method of rye bran-derived feruloyl arabinoxylans, which comprises the following steps of: pretreating rye bran raw materials, and removing proteins and starch in the wheat bran to obtain pretreated wheat bran; mixing the pretreated wheat bran with xylanase, and performing ultrasonic auxiliary extraction to obtain an extract; and (3) separating and purifying the extract to obtain the black wheat bran source feruloyl arabinoxylan. The method adopts the ultrasonic-assisted extraction combined with the enzymatic method to extract the arabinoxylans in the wheat bran, is milder compared with the common alkaline method, has better retention effect on the ferulic acid, can effectively reduce the loss of the ferulic acid in the extraction process, and improves the content of the ferulic acid in the feruloyl arabinoxylans obtained by extraction, thereby improving the oxidation resistance and prebiotic effect of the feruloyl arabinoxylans.

Description

A kind of preparation method of rye bran source feruloyl arabinoxylan

technical field

The invention relates to the technical field of agricultural and sideline products processing, in particular to a preparation method of rye bran source feruloyl arabinoxylan.

Background technique

The currently widely used method for preparing wheat bran-derived feruloyl arabinoxylan is alkaline extraction, such as using strong alkali for extraction. However, using strong alkali to extract arabinoxylan from wheat bran will lead to arabinoxylan The ferulic acid on the xylan falls, resulting in a low or even no ferulic acid content in the extracted arabinoxylan, which makes a series of activities such as prebiotic effect and antioxidant activity of arabinoxylan reduce.

Contents of the invention

The main purpose of the present invention is to propose a method for preparing feruloyl arabinoxylan from rye bran, aiming at increasing the content of ferulic acid in the extracted feruloyl arabinoxylan.

In order to achieve the above object, the present invention proposes a method for preparing feruloyl arabinoxylan from rye bran, comprising the following steps:

Pretreating the rye bran raw material to remove protein and starch in the wheat bran to obtain pretreated wheat bran;

The pretreated wheat bran is mixed with xylanase and subjected to ultrasonic-assisted extraction to obtain an extract;

The extract is separated and purified to prepare rye bran source feruloyl arabinoxylan.

Optionally, the mass ratio of the xylanase to the rye bran raw material is 1:120-130.

Optionally, the mass ratio of the xylanase to the rye bran raw material is 1:125.

Optionally, the ultrasonic temperature of the ultrasonic-assisted extraction is 54-58° C., the ultrasonic power is 150-170 W, the ultrasonic frequency is 45-55 kHz, and the ultrasonic extraction time is 80-100 min.

Optionally, the ultrasonic temperature of the ultrasonic-assisted extraction is 56° C., the ultrasonic power is 160 W, the ultrasonic frequency is 50 kHz, and the ultrasonic extraction time is 90 min.

Optionally, the extraction solvent for the ultrasonic-assisted extraction is deionized water, and 18-22 L of deionized water is added for every 1 kg of the pretreated wheat bran.

Optionally, the rye bran raw material includes Jizi 439 rye bran.

Optionally, the rye bran raw material is rye bran powder with a particle size of 30-50 mesh.

Optionally, the step of preprocessing the rye bran raw material includes:

Use dispase to remove protein and alpha-amylase to remove starch.

Optionally, the step of separating and purifying the extract to prepare feruloyl arabinoxylan from rye bran includes:

After the extract is centrifuged, a supernatant is taken, and the supernatant is dialyzed, concentrated, and freeze-dried to prepare feruloyl arabinoxylan from rye bran.

In the technical scheme provided by the present invention, the feruloyl arabinoxylan in the rye bran is extracted by ultrasonic-assisted extraction combined with an enzymatic method, which is milder than the commonly used alkaline method and has a better effect on ferulic acid. The retention effect can effectively reduce the loss of ferulic acid in the extraction process, increase the content of ferulic acid in the extracted arabinoxylan, thereby improving the antioxidant activity and prebiotic effect of arabinoxylan.

Description of drawings

In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings that need to be used in the description of the embodiments or the prior art. Obviously, the accompanying drawings in the following description are only For some embodiments of the present invention, those skilled in the art can also obtain other related drawings according to these drawings without any creative effort.

Fig. 1 is a schematic flow diagram of an embodiment of the preparation method of rye bran-derived feruloyl arabinoxylan provided by the present invention.

The realization of the purpose of the present invention, functional characteristics and advantages will be further described in conjunction with the embodiments and with reference to the accompanying drawings.

Detailed ways

In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. Those who do not indicate the specific conditions in the examples are carried out according to the conventional conditions or the conditions suggested by the manufacturer. The reagents or instruments used were not indicated by the manufacturer, and they were all conventional products that could be purchased from the market. In addition, the meaning of "and/or" appearing throughout the text includes three parallel schemes, taking "A and/or B" as an example, including scheme A, scheme B, or schemes in which A and B are satisfied at the same time. In addition, the technical solutions of the various embodiments can be combined with each other, but it must be based on the realization of those skilled in the art. When the combination of technical solutions is contradictory or cannot be realized, it should be considered that the combination of technical solutions does not exist , nor within the scope of protection required by the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of the present invention.

The currently widely used method for preparing wheat bran-derived feruloyl arabinoxylan is alkaline extraction, such as using strong alkali for extraction. However, using strong alkali to extract arabinoxylan from wheat bran will lead to arabinoxylan The ferulic acid on the xylan falls, resulting in a low or even no ferulic acid content in the extracted arabinoxylan, which makes a series of activities such as prebiotic effect and antioxidant activity of arabinoxylan reduce. The inventor found through research that the content of ferulic acid in rye bran is higher than that of white wheat bran. Based on this, the present invention proposes a method for preparing feruloyl arabinoxylan from rye bran, as shown in Figure 1 An embodiment of the preparation method of feruloyl arabinoxylan derived from rye bran provided by the present invention.

Referring to Fig. 1, in the present embodiment, the preparation method of the rye bran source feruloyl arabinoxylan comprises the following steps:

Step S10, pretreating the rye bran raw material to remove protein and starch in the wheat bran to obtain pretreated wheat bran;

Step S20, mixing the pretreated wheat bran with xylanase and performing ultrasonic-assisted extraction to obtain an extract;

Step S30, separating and purifying the extract to prepare feruloyl arabinoxylan from rye bran.

Firstly, the rye bran raw material is pretreated, and the protein and starch are removed from the rye bran raw material to obtain the pretreated wheat bran; then it is mixed with xylanase, and extracted by enzymatic method combined with ultrasonic assisted extraction , to obtain the extract from which feruloyl arabinoxylan is extracted; finally, the feruloyl arabinoxylan in the process of the extract is separated and purified to obtain the rye bran source feruloyl arabinoxylan Xylan.

In the technical scheme provided by the present invention, the feruloyl arabinoxylan in the rye bran is extracted by ultrasonic-assisted extraction combined with an enzymatic method, which is milder than the commonly used alkaline method and has a better effect on ferulic acid. The retention effect can effectively reduce the loss of ferulic acid during the extraction process, increase the content of ferulic acid in the feruloyl arabinoxylan obtained from the extraction, thereby improving the free radical scavenging properties and prebiotic effect of the arabinoxylan.

In some embodiments of the present invention, the mass ratio of the xylanase to the rye bran raw material is 1:120-130. In some specific embodiments of the present invention, the mass ratio of the xylanase to the rye bran raw material is 1:125.

In some embodiments of the present invention, the ultrasonic temperature of the ultrasonic-assisted extraction is 54-58° C., the ultrasonic power is 150-170 W, the ultrasonic frequency is 45-55 kHz, and the ultrasonic extraction time is 80-100 min. In some specific embodiments of the present invention, the ultrasonic temperature of the ultrasonic-assisted extraction is 56° C., the ultrasonic power is 160 W, the ultrasonic frequency is 50 kHz, and the ultrasonic extraction time is 90 minutes.

In some embodiments of the present invention, the extraction solvent of the ultrasonic-assisted extraction is deionized water, and 18-22 L of deionized water is added for every 1 kg of the pretreated wheat bran for ultrasonic-assisted extraction. In some embodiments of the present invention, 20 L of deionized water is added for every 1 kg of the pretreated wheat bran.

The method for extracting feruloyl arabinoxylan from rye bran provided by the present invention is basically applicable to all types of wheat currently, preferably winter wheat, more preferably Jizi 439 rye. That is, in some embodiments of the present invention, the rye bran raw material includes Jizi 439 rye bran.

In addition, in some embodiments of the present invention, the rye bran raw material is wheat bran powder with a particle size of 30-50 mesh. Using powdered wheat bran powder as a raw material is beneficial to improve the preparation efficiency of subsequent steps, such as improving the processing efficiency of protein and starch removal, and improving the extraction efficiency of feruloyl arabinoxylan. Specifically, the wheat bran can be ground first, and then passed through a 30-50 mesh sieve to obtain the wheat bran powder.

In some embodiments of the present invention, the step of pretreating the rye bran raw material in step S10 includes: using neutral protease to remove protein, and using α-amylase to remove starch.

The method of separating the feruloyl arabinoxylan in the extract can be to firstly separate the solid matter in the extract by solid-liquid methods such as centrifugation and filtration, and then carry out further refining by dialysis and concentration. Finally, dry to remove moisture. Specifically, in some embodiments of the present invention, the step S30 includes: centrifuging the extract and taking the supernatant, dialyzing, concentrating, and freeze-drying the supernatant to obtain rye bran source A Feruloyl arabinoxylan.

The technical solutions of the present invention will be described in further detail below in conjunction with specific embodiments and accompanying drawings. It should be understood that the following embodiments are only used to explain the present invention and are not intended to limit the present invention.

Example 1

(1) Grinding Jizi 439 rye bran and passing through a 40-mesh sieve to obtain wheat bran powder, then using neutral protease to remove protein and α-amylase to remove starch to obtain pretreated wheat bran.

(2) According to the quality of xylanase: the mass ratio of wheat bran powder quality=1:125, the wheat bran after pretreatment is mixed with xylanase, then with deionized water as solvent (per 1kg of pretreated Add 20L of water to the wheat bran), and use an ultrasonic extractor to perform ultrasonic-assisted extraction to obtain the extract. The time is 90 minutes.

(3) Centrifuge the obtained extract, collect the supernatant, dialyze, concentrate and freeze-dry the supernatant to prepare feruloyl arabinoxylan from rye bran.

After testing, the content of ferulic acid in feruloyl arabinoxylan derived from rye bran obtained from extraction was 13.26 μg/mg.

Example 2

(1) Grinding Jizi 439 rye bran and passing through a 30-mesh sieve to obtain wheat bran powder, then using neutral protease to remove protein and α-amylase to remove starch to obtain pretreated wheat bran.

(2) According to the quality of xylanase: the mass ratio of wheat bran powder quality=1:120, the wheat bran after pretreatment is mixed with xylanase, then with deionized water as solvent (per 1kg of pretreated Add 18L of water to the wheat bran), and use an ultrasonic extractor to perform ultrasonic-assisted extraction to obtain the extract. The time is 80 minutes.

(3) Centrifuge the obtained extract, collect the supernatant, dialyze, concentrate and freeze-dry the supernatant to prepare feruloyl arabinoxylan from rye bran.

After detection, the content of ferulic acid in feruloyl arabinoxylan derived from rye bran obtained from extraction was 11.96 μg/mg.

Example 3

(1) Grinding Jizi 439 rye bran and passing through a 35-mesh sieve to obtain wheat bran powder, then using neutral protease to remove protein and α-amylase to remove starch to obtain pretreated wheat bran.

(2) According to the quality of xylanase: the mass ratio of wheat bran powder quality=1:122, the wheat bran after pretreatment is mixed with xylanase, then with deionized water as solvent (per 1kg of pretreated Add 22L of water to the wheat bran), and use an ultrasonic extractor to perform ultrasonic-assisted extraction to obtain the extract. The time is 85 minutes.

(3) Centrifuge the obtained extract, collect the supernatant, dialyze, concentrate and freeze-dry the supernatant to prepare feruloyl arabinoxylan from rye bran.

After detection, the content of ferulic acid in feruloyl arabinoxylan derived from rye bran obtained from extraction was 12.64 μg/mg.

Example 4

(1) Grinding Jizi 439 rye bran and passing through a 45-mesh sieve to obtain wheat bran powder, then using neutral protease to remove protein and α-amylase to remove starch to obtain pretreated wheat bran.

(2) According to the quality of xylanase: the mass ratio of wheat bran powder quality=1:128, the wheat bran after pretreatment is mixed with xylanase, then with deionized water as solvent (per 1kg of pretreated Add 20L of water to the wheat bran), and use an ultrasonic extractor to perform ultrasonic-assisted extraction to obtain the extract. The time is 95 minutes.

(3) Centrifuge the obtained extract, collect the supernatant, dialyze, concentrate and freeze-dry the supernatant to prepare feruloyl arabinoxylan from rye bran.

After testing, the content of ferulic acid in feruloyl arabinoxylan derived from rye bran obtained from extraction was 13.08 μg/mg.

Example 5

(1) Grinding Jizi 439 rye bran and passing through a 50-mesh sieve to obtain wheat bran powder, then using neutral protease to remove protein and α-amylase to remove starch to obtain pretreated wheat bran.

(2) According to the quality of xylanase: the mass ratio of wheat bran powder quality=1:130, the wheat bran after pretreatment is mixed with xylanase, then with deionized water as solvent (per 1kg of pretreated Add 20L of water to the wheat bran), and use an ultrasonic extractor to perform ultrasonic-assisted extraction to obtain the extract. The time is 100min.

(3) Centrifuge the obtained extract, collect the supernatant, dialyze, concentrate and freeze-dry the supernatant to prepare feruloyl arabinoxylan from rye bran.

After testing, the content of ferulic acid in feruloyl arabinoxylan derived from rye bran obtained from extraction was 12.15 μg/mg.

Comparative example 1

The extraction method of wheat bran source arabinoxylan refers to the prior art 1 (Wang L, Zhang L, Qiu S, et al. Rheological properties and structural characteristics of arabinoxylanhydrogels prepared from three wheat bran sources[J]. Journal of cerealscience, 2019, 88:79-86) proceed.

The content of ferulic acid in the extracted wheat bran-derived arabinoxylan was 7.39 μg/mg.

Comparative example 2

The extraction method of wheat bran source arabinoxylans refers to prior art 2 (Skendi A, Biliaderis C G, Izydorczyk M S, et al.Structural variation and rheological properties of water-extractable arabinoxylans from six Greek wheat cultivars[J].FoodChemistry,2011, 126(2):526-536).

The content of ferulic acid in the extracted wheat bran source arabinoxylan is 0.95 μg/mg.

The above are only preferred embodiments of the present invention, and are not intended to limit the patent scope of the present invention. For those skilled in the art, the present invention may have various modifications and changes. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention shall be included in the patent protection scope of the present invention.

Claims (10)

1. a preparation method of rye bran source feruloyl arabinoxylan, is characterized in that, comprises the following steps: Pretreating the rye bran raw material to remove protein and starch in the wheat bran to obtain pretreated wheat bran; The pretreated wheat bran is mixed with xylanase and subjected to ultrasonic-assisted extraction to obtain an extract; The extract is separated and purified to prepare rye bran source feruloyl arabinoxylan. 2. The method for preparing feruloyl arabinoxylan from rye bran as claimed in claim 1, wherein the mass ratio of the xylanase to the rye bran raw material is 1:120-130 . 3. The method for preparing feruloyl arabinoxylan from rye bran as claimed in claim 2, wherein the mass ratio of the xylanase to the rye bran raw material is 1:125. 4. The method for preparing feruloyl arabinoxylan derived from rye bran as claimed in claim 1, characterized in that, the ultrasonic temperature of the ultrasonic-assisted extraction is 54-58°C, the ultrasonic power is 150-170W, and the ultrasonic The frequency is 45-55 kHz, and the ultrasonic extraction time is 80-100 min. 5. The preparation method of rye bran source feruloyl arabinoxylan as claimed in claim 4, characterized in that, the ultrasonic temperature of the ultrasonic-assisted extraction is 56°C, the ultrasonic power is 160W, and the ultrasonic frequency is 50kHz, Ultrasonic extraction time is 90min. 6. The preparation method of feruloyl arabinoxylan of rye bran source as claimed in claim 1, is characterized in that, the extraction solvent of described ultrasonic assisted extraction is deionized water, every 1kg described pretreated wheat Add 18-22L of deionized water to the bran. 7. The method for preparing feruloyl arabinoxylan from rye bran as claimed in claim 1, wherein the rye bran raw material comprises Jizi 439 rye bran. 8 . The method for preparing feruloyl arabinoxylan derived from rye bran according to claim 1 , wherein the rye bran raw material is rye bran powder with a particle size of 30-50 mesh. 9. The preparation method of rye bran source feruloyl arabinoxylan as claimed in claim 1, is characterized in that, the step of pretreating rye bran raw material comprises: Use dispase to remove protein and alpha-amylase to remove starch. 10. The preparation method of rye bran source feruloyl arabinoxylan as claimed in claim 1, characterized in that, the extract is separated and purified to obtain rye bran source feruloyl arabinoxylan steps, including: After the extract is centrifuged, a supernatant is taken, and the supernatant is dialyzed, concentrated, and freeze-dried to prepare feruloyl arabinoxylan from rye bran.

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