CN116270906A - Jerusalem artichoke flower compound solid essential oil for assisting in improving memory and preparation method thereof - Google Patents

Jerusalem artichoke flower compound solid essential oil for assisting in improving memory and preparation method thereof Download PDF

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CN116270906A
CN116270906A CN202310019554.5A CN202310019554A CN116270906A CN 116270906 A CN116270906 A CN 116270906A CN 202310019554 A CN202310019554 A CN 202310019554A CN 116270906 A CN116270906 A CN 116270906A
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essential oil
oil
jerusalem artichoke
mixture
artichoke flower
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左金朋
田宝兰
周国梅
范佳
张森
江自建
张涛
翟淑然
汪洋
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Taian Taishan Innovation Valley Technology Operation Management Co ltd
Shandong Yidelai Biotechnology Co ltd
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Shandong Yidelai Biotechnology Co ltd
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Abstract

The invention discloses jerusalem artichoke flower compound solid essential oil for assisting in improving memory, which is prepared from natural high polymer resin, wax base, oil base, liquid compound essential oil and volatile auxiliary agent, wherein the liquid compound essential oil consists of jerusalem artichoke flower essential oil, vetiver essential oil, basil essential oil, jasmine essential oil and bay essential oil. The preparation method of the jerusalem artichoke flower compound solid essential oil comprises the following steps: mixing Jerusalem artichoke flower essential oil, vetiver essential oil, basil essential oil, jasmine essential oil and laurel essential oil to obtain liquid compound essential oil; and (3) fusing natural polymer resin, wax base, oil base and volatile auxiliary agent to obtain a mixture, adding the liquid compound essential oil into the mixture, and standing and curing at room temperature to obtain the jerusalem artichoke flower compound solid essential oil. The jerusalem artichoke flower compound solid essential oil has obvious promotion effect on improving memory through combination of jerusalem artichoke flower essential oil, vetiver essential oil, basil essential oil, jasmine essential oil and bay essential oil.

Description

Jerusalem artichoke flower compound solid essential oil for assisting in improving memory and preparation method thereof
Technical Field
The invention relates to the technical field of essential oil, in particular to jerusalem artichoke flower compound solid essential oil for assisting in improving memory and a preparation method thereof.
Background
Along with the continuous acceleration of modern life rhythm, the influence of long-term life pressure, working pressure, smoking, alcoholism, lack of movement and other bad life habits causes serious sleep time deficiency of people, so that each item of human body is disordered, and the phenomenon of hypolearning and memory capacity is increasingly plagued for modern people. Memory is the recognition, preservation, reproduction, or recongnition of things experienced by the human brain, which is the basis for advanced mental activities such as thinking, imagination, etc. When the brain is overloaded, the timely absorption and supplementation of oxygen and nutrition by brain cells are affected, so that brain fatigue is caused, and memory deterioration is caused. At the same time, mental fatigue, anxiety and dysphoria are more serious with the memory decline. For a long time, the immune function of the body is reduced and various diseases are induced. Due to memory impairment caused by brain overfatigue or anxiety, some useful measures can be taken to alleviate and improve the memory decline phenomenon caused by such brain dysfunction.
The essential oil is extracted from leaves, flowers, seeds, fruits, roots, barks, resins, wood cores and other parts of plants by steam distillation, cold pressing, fat absorption and solvent extraction, and has high-concentration aromatic and volatile greasy liquid. Essential oils are lipophilic and readily soluble in oils because the molecular chains of essential oils are generally relatively short, which makes them very permeable to the skin and enter the body via the abundant capillaries under subcutaneous fat. Essential oils are composed of very small molecules, which are absorbed by the mucosal tissues of the nasal cavity into the body, and send messages directly to the brain, through the limbic system of the brain, regulating emotion and physiological functions of the body. So in aromatherapy, the essential oils can enhance physiological and psychological functions.
However, the functional value and the processing technology of jerusalem artichoke flowers are not fully researched and developed at present, and research on the use of jerusalem artichoke flower essential oil for improving memory is not reported yet.
Disclosure of Invention
Aiming at the prior art, the invention aims to provide jerusalem artichoke flower compound solid essential oil for assisting in improving memory and a preparation method thereof.
In order to achieve the above purpose, the invention adopts the following technical scheme:
in a first aspect of the invention, jerusalem artichoke flower compound solid essential oil for assisting in improving memory is provided, and is prepared from the following raw materials:
natural polymer resin, wax base, oil base, volatile auxiliary agent and liquid compound essential oil.
Preferably, the mass ratio of the natural polymer resin to the wax base is (2-5): (2-4).
Preferably, the mass ratio of the total mass of the natural polymer resin and the wax base to the oil base is 9:1.
Preferably, the mass ratio of the total mass of the natural high polymer resin, the wax base and the oil base to the volatile auxiliary agent is 10: (3-4).
Preferably, the mass ratio of the total mass of the natural high polymer resin, the wax base, the oil base and the volatile auxiliary agent to the liquid compound essential oil is 10: (2-3).
Preferably, the liquid compound essential oil consists of the following raw materials in parts by weight: 5-15 parts of jerusalem artichoke flower essential oil, 2-10 parts of vetiver essential oil, 2-5 parts of basil essential oil, 1-2 parts of jasmine essential oil and 1-2 parts of laurel essential oil.
Preferably, the natural polymer resin is dammar resin or Lepidium resin.
Preferably, the wax base is one of white beeswax, corn wax or rice bran wax.
Preferably, the volatilizing aid is corn alcohol or sorghum alcohol.
Preferably, the oil base is one of palm oil, coconut oil or olive oil.
The invention provides a preparation method of jerusalem artichoke flower compound solid essential oil for assisting in improving memory, which comprises the following steps of:
(1) Uniformly mixing Jerusalem artichoke flower essential oil, vetiver essential oil, basil essential oil, jasmine essential oil and laurel essential oil to obtain liquid compound essential oil;
(2) Heating and melting natural polymer resin, adding wax base, mixing uniformly to obtain a molten mixture, adding oil base into the molten mixture, stopping heating after mixing uniformly, cooling to room temperature, adding a volatilizing auxiliary agent, and stirring to obtain a mixture;
(3) Adding the liquid compound essential oil in the step (1) into the mixture in the step (2) under stirring, and standing and solidifying at room temperature to obtain the jerusalem artichoke flower compound solid essential oil.
Preferably, in the step (1), the preparation method of the jerusalem artichoke flower essential oil comprises the following steps: pulverizing Jerusalem artichoke flower, adding 5% NaCl solution, distilling, condensing to obtain oil-water mixture, standing, separating oil phase, and drying to obtain Jerusalem artichoke flower essential oil.
Further preferably, the mass ratio of the crushed jerusalem artichoke flowers to the NaCl solution is 1:20.
Further preferably, the distillation temperature is 95 to 100℃and the distillation time is 5 hours.
Further preferably, the drying method is as follows: the oil phase was dried with anhydrous sodium sulfate.
Preferably, in the step (1), the preparation method of the vetiver essential oil comprises the following steps: crushing vetiver, adding a NaCl solution with the mass fraction of 3%, distilling and condensing to obtain an oil-water mixture, standing the oil-water mixture, separating an oil phase, and drying to obtain the vetiver essential oil.
Further preferably, the mass ratio of the crushed vetiver to the NaCl solution is 1:15.
More preferably, the distillation temperature is 100 to 105℃and the distillation time is 3 hours.
Further preferably, the drying method is as follows: the oil phase was dried with anhydrous sodium sulfate.
Preferably, in the step (1), the preparation method of the basil essential oil comprises the following steps: pulverizing herba Ocimi, adding 3% NaCl solution, distilling, condensing to obtain oil-water mixture, standing, separating oil phase, and drying to obtain herba Ocimi essential oil.
Further preferably, the mass ratio of the crushed basil to the NaCl solution is 1:15.
More preferably, the distillation temperature is 100 to 105℃and the distillation time is 3 hours.
Further preferably, the drying method is as follows: the oil phase was dried with anhydrous sodium sulfate.
Preferably, in the step (1), the preparation method of the jasmine essential oil comprises the following steps: crushing jasmine, adding a NaCl solution with the mass fraction of 2%, distilling and condensing to obtain an oil-water mixture, standing the oil-water mixture, separating an oil phase, and drying to obtain the jasmine essential oil.
Further preferably, the mass ratio of the crushed jasmine to the NaCl solution is 1:20.
Further preferably, the distillation temperature is 105 to 110℃and the distillation time is 2 hours.
Further preferably, the drying method is as follows: the oil phase was dried with anhydrous sodium sulfate.
Preferably, in the step (1), the preparation method of the laurel essential oil comprises the following steps: pulverizing laurel, adding 4% NaCl solution, distilling, condensing to obtain oil-water mixture, standing, separating oil phase, and drying to obtain laurel essential oil.
Further preferably, the mass ratio of the crushed laurel to the NaCl solution is 1:15.
More preferably, the distillation temperature is 100 to 105℃and the distillation time is 3 hours.
Further preferably, the drying method is as follows: the oil phase was dried with anhydrous sodium sulfate.
The invention has the beneficial effects that:
the jerusalem artichoke flower contains rich flavonoids, anthocyanidins, chlorogenic acid, proteins, amino acids, vitamins, and rich trace elements such as calcium, magnesium, iron, zinc, manganese, selenium and the like which are essential nutrient components of human bodies, and has the effects of resisting bacteria, diminishing inflammation, resisting mutation, reducing blood glucose, reducing blood pressure and blood lipid, maintaining beauty and keeping young, regulating the color of qi and the like; the laurel fibrous root contains aromatic and strong volatile fragrance, has the effect of calming, can lead people to have mild mood and stable emotion, can lead the oxygen carrying capacity of the red blood balls of the human body to be improved, and can lead the endocrine system to be maintained and even strengthened, and can lead the people to face with calm mood under the face of strong pressure; the basil relieves pressure when the nerves are tired, and excites emotion, so that the depression is effectively improved; the chemical substances in the laurel can generate a sedative and relaxing effect, which can help to restore the state of heart-qi and the calm effect of the laurel can not lead people to want to sleep, but can help to eliminate fatigue; jasmine is an effective drug for treating severe mental depression, can sooth nerves, establish a self-confidence, have a feeling of optimism and mental well-being, and can also regenerate and restore energy.
The jerusalem artichoke flower, the laurel, the basil, the jasmine and the laurel are respectively distilled and condensed to obtain the corresponding essential oil, and the essential oil is mixed according to a certain proportion, so that the effect of improving the memory is obvious due to the effect of a single essential oil; meanwhile, the five essential oils are prepared into a solid essential oil form, so that volatilization of the essential oils is avoided to a great extent.
Detailed Description
It should be noted that the following detailed description is illustrative and is intended to provide further explanation of the present application. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs.
In order to enable those skilled in the art to more clearly understand the technical solutions of the present application, the technical solutions of the present application will be described in detail below with reference to specific embodiments.
The test materials used in the examples of the present invention are all conventional in the art and are commercially available.
Example 1
(1) 5 parts of jerusalem artichoke flower essential oil, 2 parts of vetiver essential oil, 2 parts of basil essential oil, 1 part of jasmine essential oil and 1 part of laurel essential oil are mixed to obtain liquid compound essential oil;
(2) Placing dammar resin powder which is crushed in advance into a culture dish, heating the culture dish on a heating stirrer until the dammar resin is melted, adding white beeswax into the culture dish in which the dammar resin is melted, stirring until the white beeswax is completely melted, adding palm oil, wherein the mass ratio of the palm oil to the total mass ratio of the dammar resin powder and the white beeswax is 1:9, fully stirring after the palm oil is completely melted, until the mixture is uniformly fused, placing the mixture at normal temperature, adding corn alcohol, wherein the mass ratio of the corn alcohol to the dammar resin powder, the white beeswax and the palm oil is 3:10, and fully stirring to obtain the mixture;
(3) Adding the liquid compound essential oil in the step (1) into the mixture in the step (2) under stirring, wherein the mass ratio of the liquid compound essential oil to the mixture is 2:10, and standing and solidifying at room temperature to obtain the jerusalem artichoke flower compound solid essential oil.
The preparation method of the jerusalem artichoke flower essential oil comprises the following steps: cleaning Jerusalem artichoke flower, pulverizing to obtain Jerusalem artichoke flower powder, placing Jerusalem artichoke flower powder and 5% NaCl solution in a container of a steam distillation device, and distilling at 95deg.C for 5 hr, wherein the mass ratio of Jerusalem artichoke flower powder to NaCl solution is 1:20, condensing essential oil components in jerusalem artichoke flowers when the essential oil components flow through a condenser to obtain an oil-water mixture; collecting and standing the oil-water mixture, separating oil phase, and drying with anhydrous sodium sulfate to obtain Jerusalem artichoke flower essential oil.
Preparing vetiver essential oil: washing and crushing vetiver to form vetiver powder, placing the vetiver powder and NaCl solution with the mass fraction of 3% into a container of a steam distillation device, and distilling for 3 hours at 100 ℃, wherein the mass ratio of the vetiver powder to the NaCl solution is 1:15, condensing essential oil components in the vetiver when the essential oil components flow through a condenser to obtain an oil-water mixture; and (3) collecting and standing the oil-water mixture, separating an oil phase, and drying the oil phase with anhydrous sodium sulfate to obtain the vetiver essential oil.
Preparing basil essential oil: cleaning and crushing basil to form basil powder, placing the basil powder and NaCl solution with the mass fraction of 3% into a container of a steam distillation device, and distilling for 3 hours at 100 ℃, wherein the mass ratio of the basil powder to the NaCl solution is 1:15, condensing the essential oil component in the basil when flowing through a condenser to obtain an oil-water mixture, collecting and standing the oil-water mixture, separating an oil phase and drying the oil phase with anhydrous sodium sulfate to obtain the basil essential oil.
Preparing jasmine essential oil: washing jasmine, crushing to form jasmine powder, placing the jasmine powder in a container of a steam distillation device in a NaCl solution with the mass fraction of 2%, and distilling at 105 ℃ for 2 hours, wherein the mass fraction of the jasmine powder and the NaCl solution is 1:20, condensing essential oil components in the jasmine when flowing through a condenser to obtain an oil-water mixture; and (3) collecting and standing the oil-water mixture, separating an oil phase, and drying the oil phase with anhydrous sodium sulfate to obtain the jasmine essential oil.
Preparing laurel essential oil: cleaning and crushing laurel to form laurel powder, placing the laurel powder and NaCl solution with the mass fraction of 4% into a container of a steam distillation device, and distilling for 3 hours at 100 ℃, wherein the mass ratio of the laurel powder to the NaCl solution is 1:15, condensing the essential oil component in the laurel while flowing through a condenser to obtain an oil-water mixture; and (3) collecting and standing the oil-water mixture, separating an oil phase, and drying the oil phase with anhydrous sodium sulfate to obtain the laurel essential oil.
Example 2
(1) 8 parts of jerusalem artichoke flower essential oil, 5 parts of vetiver essential oil, 3 parts of basil essential oil, 1 part of jasmine essential oil and 1 part of laurel essential oil are mixed to obtain liquid compound essential oil;
(2) Putting the powdered Lepidium resin which is crushed in advance into a culture dish, heating the culture dish on a heating stirrer until the Lepidium resin is melted, adding corn wax into the culture dish with the Lepidium resin, wherein the mass ratio of the Lepidium resin powder to the corn wax is 3.5:3, stirring until the corn wax is completely melted, adding coconut oil, fully stirring the mixture after the coconut oil is completely melted until the mixture is uniformly fused, adding sorghum alcohol, and fully stirring the mixture to obtain a mixture after the mass ratio of the sorghum alcohol to the Lepidium resin powder, the corn wax and the coconut oil is 3.5:10;
(3) Adding the liquid compound essential oil in the step (1) into the mixture in the step (2) under stirring, wherein the mass ratio of the liquid compound essential oil to the mixture is 2.5:10, and standing and solidifying at room temperature to obtain the jerusalem artichoke flower compound solid essential oil.
The preparation method of the jerusalem artichoke flower essential oil comprises the following steps: cleaning Jerusalem artichoke flower, pulverizing to obtain Jerusalem artichoke flower powder, placing Jerusalem artichoke flower powder and 5% NaCl solution in a container of a steam distillation device, and distilling at 97deg.C for 5 hr, wherein the mass ratio of Jerusalem artichoke flower powder to NaCl solution is 1:20, condensing essential oil components in jerusalem artichoke flowers when the essential oil components flow through a condenser to obtain an oil-water mixture; collecting and standing the oil-water mixture, separating oil phase, and drying with anhydrous sodium sulfate to obtain Jerusalem artichoke flower essential oil.
Preparing vetiver essential oil: washing and crushing vetiver to form vetiver powder, placing the vetiver powder and NaCl solution with the mass fraction of 3% into a container of a steam distillation device, and distilling for 3 hours at 102 ℃, wherein the mass ratio of the vetiver powder to the NaCl solution is 1:15, condensing essential oil components in the vetiver when the essential oil components flow through a condenser to obtain an oil-water mixture; and (3) collecting and standing the oil-water mixture, separating an oil phase, and drying the oil phase with anhydrous sodium sulfate to obtain the vetiver essential oil.
Preparing basil essential oil: cleaning and crushing basil to form basil powder, placing the basil powder and NaCl solution with the mass fraction of 3% into a container of a steam distillation device, and distilling for 3 hours at 102 ℃, wherein the mass ratio of the basil powder to the NaCl solution is 1:15, condensing the essential oil component in the basil when flowing through a condenser to obtain an oil-water mixture, collecting and standing the oil-water mixture, separating an oil phase and drying the oil phase with anhydrous sodium sulfate to obtain the basil essential oil.
Preparing jasmine essential oil: washing jasmine, crushing to form jasmine powder, placing the jasmine powder and NaCl solution with the mass fraction of 2% into a container of a steam distillation device, and distilling for 2 hours at 108 ℃, wherein the mass ratio of the jasmine powder to the NaCl solution is 1:20, condensing essential oil components in the jasmine when flowing through a condenser to obtain an oil-water mixture; and (3) collecting and standing the oil-water mixture, separating an oil phase, and drying the oil phase with anhydrous sodium sulfate to obtain the jasmine essential oil.
Preparing laurel essential oil: cleaning and crushing laurel to form laurel powder, placing the laurel powder and NaCl with the mass fraction of 4% in a container of a steam distillation device, and distilling for 3 hours at 103 ℃ with the mass ratio of the laurel powder to NaCl solution of 1:15, condensing the essential oil component in the laurel while flowing through a condenser to obtain an oil-water mixture; and (3) collecting and standing the oil-water mixture, separating an oil phase, and drying the oil phase with anhydrous sodium sulfate to obtain the laurel essential oil.
Example 3
(1) 10 parts of jerusalem artichoke flower essential oil, 8 parts of vetiver essential oil, 4 parts of basil essential oil, 1.5 parts of jasmine essential oil and 1.5 parts of bay essential oil are mixed to obtain liquid compound essential oil;
(2) Placing dammar resin powder which is crushed in advance into a culture dish, heating the culture dish on a heating stirrer until the dammar resin is melted, adding white beeswax into the culture dish with the dammar resin, wherein the mass ratio of the dammar resin powder to the white beeswax is 4:3, stirring the mixture by using a glass rod until the white beeswax is completely melted, adding palm oil, fully stirring the mixture after the palm oil is completely melted until the mixture is uniformly fused, adding corn alcohol, wherein the mass ratio of the corn alcohol to the dammar resin powder to the white beeswax to the palm oil is 3.5:10, and fully stirring the mixture to obtain a mixture;
(3) Adding the liquid compound essential oil in the step (1) into the mixture in the step (2) under stirring, wherein the mass ratio of the liquid compound essential oil to the mixture is 2.5:10, and standing and solidifying at room temperature to obtain the jerusalem artichoke flower compound solid essential oil.
The preparation method of the jerusalem artichoke flower essential oil comprises the following steps: cleaning Jerusalem artichoke flower, pulverizing to obtain Jerusalem artichoke flower powder, placing Jerusalem artichoke flower powder and 5% NaCl solution in a container of a steam distillation device, and distilling at 97deg.C for 5 hr, wherein the mass ratio of Jerusalem artichoke flower powder to NaCl solution is 1:20, condensing essential oil components in jerusalem artichoke flowers when the essential oil components flow through a condenser to obtain an oil-water mixture; collecting and standing the oil-water mixture, separating oil phase, and drying with anhydrous sodium sulfate to obtain Jerusalem artichoke flower essential oil.
Preparing vetiver essential oil: washing and crushing vetiver to form vetiver powder, placing the vetiver powder and NaCl solution with the mass fraction of 3% into a container of a steam distillation device, and distilling for 3 hours at 102 ℃, wherein the mass ratio of the vetiver powder to the NaCl solution is 1:15, condensing essential oil components in the vetiver when the essential oil components flow through a condenser to obtain an oil-water mixture; and (3) collecting and standing the oil-water mixture, separating an oil phase, and drying the oil phase with anhydrous sodium sulfate to obtain the vetiver essential oil.
Preparing basil essential oil: cleaning and crushing basil to form basil powder, placing the basil powder and NaCl solution with the mass fraction of 3% into a container of a steam distillation device, and distilling for 3 hours at 102 ℃, wherein the mass ratio of the basil powder to the NaCl solution is 1:15, condensing the essential oil component in the basil when flowing through a condenser to obtain an oil-water mixture, collecting and standing the oil-water mixture, separating an oil phase and drying the oil phase with anhydrous sodium sulfate to obtain the basil essential oil.
Preparing jasmine essential oil: washing jasmine, crushing to form jasmine powder, placing the jasmine powder and NaCl solution with the mass fraction of 2% into a container of a steam distillation device, and distilling for 2 hours at 108 ℃, wherein the mass ratio of the jasmine powder to the NaCl solution is 1:20, condensing essential oil components in the jasmine when flowing through a condenser to obtain an oil-water mixture; and (3) collecting and standing the oil-water mixture, separating an oil phase, and drying the oil phase with anhydrous sodium sulfate to obtain the jasmine essential oil.
Preparing laurel essential oil: cleaning and crushing laurel to form laurel powder, placing the laurel powder and NaCl with the mass fraction of 4% in a container of a steam distillation device, and distilling for 3 hours at 103 ℃ with the mass ratio of the laurel powder to NaCl solution of 1:15, condensing the essential oil component in the laurel while flowing through a condenser to obtain an oil-water mixture; and (3) collecting and standing the oil-water mixture, separating an oil phase, and drying the oil phase with anhydrous sodium sulfate to obtain the laurel essential oil.
Example 4
(1) 15 parts of jerusalem artichoke flower essential oil, 10 parts of vetiver essential oil, 5 parts of basil essential oil, 2 parts of jasmine essential oil and 2 parts of laurel essential oil are mixed to obtain liquid compound essential oil;
(2) Placing dammar resin powder which is crushed in advance into a culture dish, heating the culture dish on a heating stirrer until the dammar resin is melted, adding rice bran wax into the culture dish with the dammar resin, wherein the mass ratio of the dammar resin powder to the rice bran wax is 5:4, stirring the mixture by using a glass rod until the rice bran wax is completely melted, adding olive oil, fully stirring the mixture after the olive oil is completely melted until the mixture is uniformly fused, adding corn alcohol, wherein the mass ratio of the corn alcohol to the dammar resin powder to the rice bran wax to the olive oil is 4:10, and fully stirring the mixture to obtain a mixture;
(3) Adding the liquid compound essential oil in the step (1) into the mixture in the step (2) under stirring, wherein the mass ratio of the liquid compound essential oil to the mixture is 3:10, and standing and solidifying at room temperature to obtain the jerusalem artichoke flower compound solid essential oil.
The preparation method of the jerusalem artichoke flower essential oil comprises the following steps: cleaning Jerusalem artichoke flower, pulverizing to obtain Jerusalem artichoke flower powder, placing Jerusalem artichoke flower powder and 5% NaCl solution in a container of a steam distillation device, and distilling at 100deg.C for 5 hr, wherein the mass ratio of Jerusalem artichoke flower powder to NaCl solution is 1:20, condensing essential oil components in jerusalem artichoke flowers when the essential oil components flow through a condenser to obtain an oil-water mixture; collecting and standing the oil-water mixture, separating oil phase, and drying with anhydrous sodium sulfate to obtain Jerusalem artichoke flower essential oil.
Preparing vetiver essential oil: washing and crushing vetiver to form vetiver powder, placing the vetiver powder and NaCl solution with the mass fraction of 3% into a container of a steam distillation device, and distilling for 3 hours at 105 ℃, wherein the mass ratio of the vetiver powder to the NaCl solution is 1:15, condensing essential oil components in the vetiver when the essential oil components flow through a condenser to obtain an oil-water mixture; and (3) collecting and standing the oil-water mixture, separating an oil phase, and drying the oil phase with anhydrous sodium sulfate to obtain the vetiver essential oil.
Preparing basil essential oil: cleaning and crushing basil to form basil powder, placing the basil powder and NaCl solution with the mass fraction of 3% into a container of a steam distillation device, and distilling for 3 hours at 105 ℃, wherein the mass ratio of the basil powder to the NaCl solution is 1:15, condensing the essential oil component in the basil when flowing through a condenser to obtain an oil-water mixture, collecting and standing the oil-water mixture, separating an oil phase and drying the oil phase with anhydrous sodium sulfate to obtain the basil essential oil.
Preparing jasmine essential oil: washing jasmine, crushing to form jasmine powder, placing the jasmine powder and NaCl solution with the mass fraction of 2% into a container of a steam distillation device, and distilling at 110 ℃ for 2 hours, wherein the mass ratio of the jasmine powder to the NaCl solution is 1:20, condensing essential oil components in the jasmine when flowing through a condenser to obtain an oil-water mixture; and (3) collecting and standing the oil-water mixture, separating an oil phase, and drying the oil phase with anhydrous sodium sulfate to obtain the jasmine essential oil.
Preparing laurel essential oil: cleaning and crushing laurel to form laurel powder, placing the laurel powder and NaCl with the mass fraction of 4% in a container of a steam distillation device, and distilling for 3 hours at 105 ℃ with the mass ratio of the laurel powder to NaCl solution of 1:15, condensing the essential oil component in the laurel while flowing through a condenser to obtain an oil-water mixture; and (3) collecting and standing the oil-water mixture, separating an oil phase, and drying the oil phase with anhydrous sodium sulfate to obtain the laurel essential oil.
Comparative example 1
(1) Placing dammar resin powder which is crushed in advance into a culture dish, heating the culture dish on a heating stirrer until the dammar resin is melted, adding white beeswax into the culture dish with the dammar resin, wherein the mass ratio of the dammar resin powder to the white beeswax is 4:3, stirring the mixture by using a glass rod until the white beeswax is completely melted, adding palm oil, fully stirring the mixture after the palm oil is completely melted until the mixture is uniformly fused, adding corn alcohol, wherein the mass ratio of the corn alcohol to the dammar resin powder to the white beeswax to the palm oil is 3.5:10, and fully stirring the mixture to obtain a mixture;
(2) Adding Jerusalem artichoke flower essential oil into the mixture obtained in the step (1) under stirring, wherein the mass ratio of the Jerusalem artichoke flower essential oil to the mixture is 2.5:10, and standing and solidifying at room temperature to obtain the solid essential oil.
The preparation method of the jerusalem artichoke flower essential oil is the same as that of the jerusalem artichoke flower essential oil in the embodiment 3.
Comparative example 2:
(1) Placing dammar resin powder which is crushed in advance into a culture dish, heating the culture dish on a heating stirrer until the dammar resin is melted, adding white beeswax into the culture dish with the dammar resin, wherein the mass ratio of the dammar resin powder to the white beeswax is 4:3, stirring the mixture by using a glass rod until the white beeswax is completely melted, adding palm oil, fully stirring the mixture after the palm oil is completely melted until the mixture is uniformly fused, adding corn alcohol, wherein the mass ratio of the corn alcohol to the dammar resin powder to the white beeswax to the palm oil is 3.5:10, and fully stirring the mixture to obtain a mixture;
(2) Adding vetiver essential oil into the mixture obtained in the step (1) under stirring, wherein the mass ratio of the vetiver essential oil to the mixture is 2.5:10, and standing and solidifying at room temperature to obtain the solid essential oil.
The preparation method of the vetiver essential oil is the same as that of the vetiver essential oil in the embodiment 3.
Comparative example 3:
(1) Placing dammar resin powder which is crushed in advance into a culture dish, heating the culture dish on a heating stirrer until the dammar resin is melted, adding white beeswax into the culture dish with the dammar resin, wherein the mass ratio of the dammar resin powder to the white beeswax is 4:3, stirring the mixture by using a glass rod until the white beeswax is completely melted, adding palm oil, fully stirring the mixture after the palm oil is completely melted until the mixture is uniformly fused, adding corn alcohol, wherein the mass ratio of the corn alcohol to the dammar resin powder to the white beeswax to the palm oil is 3.5:10, and fully stirring the mixture to obtain a mixture;
(2) Adding the basil essential oil into the mixture in the step (1) under stirring, wherein the mass ratio of the basil essential oil to the mixture is 2.5:10, and standing and solidifying at room temperature to obtain the solid essential oil.
Wherein, the preparation method of the basil essential oil is the same as that of the basil essential oil in example 3.
Comparative example 4:
(1) Placing dammar resin powder which is crushed in advance into a culture dish, heating the culture dish on a heating stirrer until the dammar resin is melted, adding white beeswax into the culture dish with the dammar resin, wherein the mass ratio of the dammar resin powder to the white beeswax is 4:3, stirring the mixture by using a glass rod until the white beeswax is completely melted, adding palm oil, fully stirring the mixture after the palm oil is completely melted until the mixture is uniformly fused, adding corn alcohol, wherein the mass ratio of the corn alcohol to the dammar resin powder to the white beeswax to the palm oil is 3.5:10, and fully stirring the mixture to obtain a mixture;
(2) Adding jasmine essential oil into the mixture in the step (1) under stirring, wherein the mass ratio of the jasmine essential oil to the mixture is 2.5:10, and standing and solidifying at room temperature to obtain the solid essential oil.
The preparation method of the jasmine essential oil is the same as that of the jasmine essential oil in the embodiment 3.
Comparative example 5:
(1) Placing dammar resin powder which is crushed in advance into a culture dish, heating the culture dish on a heating stirrer until the dammar resin is melted, adding white beeswax into the culture dish with the dammar resin, wherein the mass ratio of the dammar resin powder to the white beeswax is 4:3, stirring the mixture by using a glass rod until the white beeswax is completely melted, adding palm oil, fully stirring the mixture after the palm oil is completely melted until the mixture is uniformly fused, adding corn alcohol, wherein the mass ratio of the corn alcohol to the dammar resin powder to the white beeswax to the palm oil is 3.5:10, and fully stirring the mixture to obtain a mixture;
(2) Adding the laurel essential oil into the mixture obtained in the step (1) under stirring, wherein the mass ratio of the laurel essential oil to the mixture is 2.5:10, and standing and solidifying at room temperature to obtain the solid essential oil.
Wherein, the preparation method of the laurel essential oil is the same as that of the laurel essential oil in example 3.
Comparative example 6:
(1) Mixing 8 parts of vetiver essential oil, 4 parts of basil essential oil, 1.5 parts of jasmine essential oil and 1.5 parts of laurel essential oil to obtain liquid compound essential oil;
(2) Placing dammar resin powder which is crushed in advance into a culture dish, heating the culture dish on a heating stirrer until the dammar resin is melted, adding white beeswax into the culture dish with the dammar resin, wherein the mass ratio of the dammar resin powder to the white beeswax is 4:3, stirring the mixture by using a glass rod until the white beeswax is completely melted, adding palm oil, fully stirring the mixture after the palm oil is completely melted until the mixture is uniformly fused, adding corn alcohol, wherein the mass ratio of the corn alcohol to the dammar resin powder to the white beeswax to the palm oil is 3.5:10, and fully stirring the mixture to obtain a mixture;
(3) Adding the liquid compound essential oil into the mixture in the step (2) under stirring, wherein the mass ratio of the liquid compound essential oil to the mixture is 2.5:10, and standing and solidifying at room temperature to obtain the solid essential oil.
The preparation method of the vetiver essential oil, the basil essential oil, the jasmine essential oil and the laurel essential oil is the same as that of the vetiver essential oil, the basil essential oil, the jasmine essential oil and the laurel essential oil in the embodiment 3.
Comparative example 7:
(1) 10 parts of jerusalem artichoke flower essential oil, 4 parts of basil essential oil, 1.5 parts of jasmine essential oil and 1.5 parts of laurel essential oil to obtain liquid compound essential oil;
(1) Placing dammar resin powder which is crushed in advance into a culture dish, heating the culture dish on a heating stirrer until the dammar resin is melted, adding white beeswax into the culture dish with the dammar resin, wherein the mass ratio of the dammar resin powder to the white beeswax is 4:3, stirring the mixture by using a glass rod until the white beeswax is completely melted, adding palm oil, fully stirring the mixture after the palm oil is completely melted until the mixture is uniformly fused, adding corn alcohol, wherein the mass ratio of the corn alcohol to the dammar resin powder to the white beeswax to the palm oil is 3.5:10, and fully stirring the mixture to obtain a mixture;
(2) Adding the liquid compound essential oil into the mixture in the step (2) under stirring, wherein the mass ratio of the liquid compound essential oil to the mixture is 2.5:10, and standing and solidifying at room temperature to obtain the solid essential oil.
The preparation methods of the jerusalem artichoke flower essential oil, the basil essential oil, the jasmine essential oil and the bay essential oil are the same as those of the jerusalem artichoke flower essential oil, the basil essential oil, the jasmine essential oil and the bay essential oil in the embodiment 3.
Experimental protocol reference: technical specification for checking and evaluating health food
Test example 1: test of mouse diving platform
Reference is made to the mouse diving tower test in patent CN 101940292B. The specific test method comprises the following steps:
SPF-class female mice, 120, weighing 20+ -3 g, were selected at 3-4 weeks of age and randomly divided into 12 groups of 10.
The control group is a solid mixture consisting of natural polymer resin, wax base, oil base and volatilizing aid in the living environment of the mice, and the specific preparation method is as follows: placing dammar resin powder which is crushed in advance into a culture dish, heating the culture dish on a heating stirrer until the dammar resin is melted, adding white beeswax into the culture dish with the dammar resin, stirring the mixture by using a glass rod until the white beeswax is completely melted, adding palm oil, fully stirring the mixture after the palm oil is completely melted until the mixture is uniformly fused, adding corn alcohol, wherein the mass ratio of the corn alcohol to the dammar resin powder to the white beeswax to the palm oil is 3.5:10, and fully stirring the mixture to obtain a solid mixture.
The test groups placed the same amount of solid essential oil of examples 1-4 and comparative examples 1-6 in the production environment of each group of mice, after the solid essential oil is slowly released, the test groups were continuously fed for 30 days, and after 30 days, the test groups of mice normally ingest and drink water freely, and the results are shown in table 1.
Jump test: the mice were placed in a reaction box for 3min, and then stimulated with 36V ac power. Then, the mice were placed on the insulating platform, and the memory acquisition latency of each mouse for the first time, the number of memory acquisition errors per mouse for the first time, and the number of animals for each group of the jumping platforms (memory acquisition) were recorded. The test was repeated 24h or 48h later, and the latency of the first jump off platform, the number of errors in the jump off platform in 3min and the number of animals (memory consolidation) of each group of jump off platforms were recorded. Training was stopped after 30 days and feeding was continued for 5 days to conduct the regression test (memory reproduction).
Table 1 mouse diving tower test results
Figure BDA0004041971950000121
As can be seen from table 1, the mice of examples 1 to 4 showed positive results in the bench test, and compared with the mice of comparative examples 1 to 7, the latency period was increased, the number of errors was reduced, and the learning and memory performance was significantly improved in the bench test, so that the effect of using five essential oil combinations of jerusalem artichoke flower essential oil, vetch essential oil, basil essential oil, jasmine essential oil and bay essential oil for improving memory was superior to the effect of each essential oil alone.
Test example 2: test for avoiding darkness of mice
Reference is made to the mouse darkness test in patent CN 101940292B. The specific test method comprises the following steps:
SPF-class female mice, 120, weighing 20+ -3 g, were selected at 3-4 weeks of age and randomly divided into 12 groups of 10.
The control group is a solid mixture consisting of natural polymer resin, wax base, oil base and volatilizing aid in the living environment of the mice, and the specific preparation method is as follows: placing dammar resin powder which is crushed in advance into a culture dish, heating the culture dish on a heating stirrer until the dammar resin is melted, adding white beeswax into the culture dish in which the dammar resin is melted, wherein the mass ratio of the dammar resin powder to the white beeswax is 4:3, stirring the mixture by using a glass rod until the white beeswax is completely melted, adding palm oil, fully stirring the mixture after the palm oil is completely melted until the mixture is uniformly fused, placing the mixture at normal temperature, adding corn alcohol, wherein the mass ratio of the corn alcohol to the dammar resin powder to the white beeswax to the palm oil is 3.5:10, and fully stirring the mixture to obtain a solid mixture after the palm oil is fully melted
Test group: the same amount of solid essential oil of examples 1 to 4 and comparative examples 1 to 6 was placed in the production environment of each group of mice, the solid essential oil was slowly released, the continuous feeding was performed for 30 days, and after 30 days, the dark-avoiding test was performed, during which time each group of mice normally ingests, and was free to drink water, and the results are shown in table 2.
Dark avoidance experiment: the face of the mouse was placed back to the hole in the darkroom while a timer was started, and the latency of each mouse into the darkroom (the time between each mouse being placed in the darkroom and being shocked into the darkroom), the number of mistakes in entering the darkroom within 5 minutes, and the number of animals (memory acquisition) of each group entering the darkroom were recorded. The test was repeated 24h or 48h later, and the latency period of each mouse entering the darkroom, the number of mistakes in entering the darkroom within 5min, and the number of animals entering the darkroom (memory consolidation) of each group were recorded. Training was stopped after 30 days and feeding was continued for 5 days to conduct the regression test (memory reproduction).
TABLE 2 results of the mice dark test
Figure BDA0004041971950000131
Figure BDA0004041971950000141
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As can be seen from table 2, the mice of examples 1 to 4 showed positive results in the dark-avoiding test, and the latency period of the mice of examples 1 to 4 was significantly prolonged and the number of mistakes was significantly reduced, so that the effect of improving memory by using five essential oils of jerusalem artichoke essential oil, vetch essential oil, basil essential oil, jasmine essential oil and laurel essential oil in the present application was superior to that of improving memory by using one essential oil alone.
Test example 3: mouse water maze test
Reference is made to the mouse water maze test in patent CN 101940292B. The specific test method comprises the following steps:
SPF-class female mice, 120, weighing 20+ -3 g, were selected at 3-4 weeks of age and randomly divided into 12 groups of 10.
The control group is that a solid mixture composed of natural polymer resin, wax base, oil base and volatilizing auxiliary agent is placed in the living environment of the mice, and the specific preparation method is as follows: placing dammar resin powder which is crushed in advance into a culture dish, heating the culture dish on a heating stirrer until the dammar resin is melted, adding white beeswax into the culture dish in which the dammar resin is melted, wherein the mass ratio of the dammar resin powder to the white beeswax is 4:3, stirring the mixture by using a glass rod until the white beeswax is completely melted, adding palm oil, fully stirring the mixture after the palm oil is completely melted until the mixture is uniformly fused, placing the mixture at normal temperature, adding corn alcohol, wherein the mass ratio of the corn alcohol to the dammar resin powder to the white beeswax to the palm oil is 3.5:10, and fully stirring the mixture to obtain a solid mixture after the palm oil is fully melted
Test group: the same amount of the solid essential oils of examples 1 to 4 and comparative examples 1 to 6 were placed in the production environment of each group of mice, the solid essential oils were slowly released, and the mice of each group were fed continuously for 30 days, and after 30 days, were subjected to the water maze test, during which time each group of mice was normally fed with water and were free to drink water, and the results are shown in table 3.
Water maze experiment: the maze lanes were 9cm deep with a water temperature of about 20 ℃ (+15℃). Mice training time was defined as 120s and mice that did not reach the endpoint within 120s were all scored as 120s.
The mice were placed near the ladder before the first training and allowed to climb up 3 times automatically. The mice are placed near the ladder and back to the stairs before each training, so that the mice can climb up 1 time automatically. The experiment is carried out in stages, and the learning score of the apparent animal is lengthened gradually. A baffle is used for stopping outside A in the first training, training is started from outside A, and the time from the point A to the end point is recorded. The second training is to lengthen the distance from the position B, the distance is trained for about 3 times until more than 80% of animals reach the end point within 2min, and the time required by each mouse to reach the end point from the position B and the number of errors are recorded (one error is calculated once entering any blind end). The last test was performed from the starting point, the mice were placed at the starting point, and the time required to reach the end point from the starting point and the number of errors occurred were recorded. During each training, the mice which do not reach the end point within 2min are guided to reach the end point and get up from the stairs of the end point, so that the training purpose is achieved. The head is directed to the starting point each time training or testing is performed. Finally, the total number of errors in each group of animals for 5 training and testing, the total time to endpoint and the total number of animals (percentage) to endpoint in 2min were calculated, and after stopping training for 5 days, the regressing experiments (memory reproduction) were performed from the starting point at different times.
TABLE 3 results of the mouse water maze test
Figure BDA0004041971950000151
Figure BDA0004041971950000161
As can be seen from table 3, the mice in examples 1 to 3 showed positive results in the water maze test, and the time for training to reach the end point was obviously shortened in the hours of examples 1 to 4, the number of crops before training reached the end point was also reduced, and the number of animals reaching the end point in training 2min was increased, so that the effect of improving memory by using the combination of five essential oils of jerusalem artichoke flower essential oil, vetiver essential oil, basil essential oil, jasmine essential oil and laurel essential oil was superior to the effect of improving memory by using a single essential oil.
The foregoing description is only of the preferred embodiments of the present application and is not intended to limit the same, but rather, various modifications and variations may be made by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principles of the present application should be included in the protection scope of the present application.

Claims (9)

1. The jerusalem artichoke flower compound solid essential oil for assisting in improving memory is characterized by comprising natural high polymer resin, wax base, oil base, volatile auxiliary agent and liquid compound essential oil;
the liquid compound essential oil consists of the following raw materials in parts by weight: 5-15 parts of jerusalem artichoke flower essential oil, 2-10 parts of vetiver essential oil, 2-5 parts of basil essential oil, 1-2 parts of jasmine essential oil and 1-2 parts of laurel essential oil.
2. The jerusalem artichoke flower compound solid essential oil for assisting in improving memory as claimed in claim 1, wherein the mass ratio of the natural high polymer resin to the wax base is (2-5): (2-4);
the mass ratio of the total mass of the natural polymer resin and the wax base to the oil base is 9:1;
the mass ratio of the total mass of the natural high polymer resin, the wax base and the oil base to the volatile auxiliary agent is 10: (3-4);
the mass ratio of the total mass of the natural high polymer resin, the wax base, the oil base and the volatile auxiliary agent to the mass ratio of the liquid compound essential oil is 10: (2-3).
3. The jerusalem artichoke flower compound solid essential oil for assisting in improving memory according to claim 1, wherein the natural high molecular resin is dammar resin or marti resin;
the wax base is one of white beeswax, corn wax or rice bran wax;
the volatilizing aid is corn alcohol or sorghum alcohol;
the oil base is one of palm oil, coconut oil or olive oil.
4. The method for preparing the jerusalem artichoke flower compound solid essential oil for assisting in improving memory according to any one of claims 1 to 3, which is characterized by comprising the following steps of:
(1) Uniformly mixing Jerusalem artichoke flower essential oil, vetiver essential oil, basil essential oil, jasmine essential oil and laurel essential oil to obtain liquid compound essential oil;
(2) Heating and melting natural polymer resin, adding wax base, stirring until the two are mixed uniformly to obtain a molten mixture, adding oil base into the molten mixture, stopping heating after the mixture is mixed uniformly, cooling to room temperature, adding a volatilizing auxiliary agent, and stirring to obtain a mixture;
(3) Adding the liquid compound essential oil in the step (1) into the mixture in the step (2) under stirring, and standing and solidifying at room temperature to obtain the jerusalem artichoke flower compound solid essential oil.
5. The method for preparing the jerusalem artichoke flower compound solid essential oil for assisting in improving memory as claimed in claim 4, wherein in the step (1), the method for preparing the jerusalem artichoke flower essential oil is as follows: pulverizing Jerusalem artichoke flower, adding 5% NaCl solution, distilling, condensing to obtain oil-water mixture, standing, separating oil phase, and drying to obtain Jerusalem artichoke flower essential oil;
the mass ratio of the crushed jerusalem artichoke flowers to the NaCl solution is 1:20;
the distillation temperature is 95-100 ℃, and the distillation time is 5h.
6. The method for preparing the jerusalem artichoke flower compound solid essential oil for assisting in improving memory as claimed in claim 4, wherein in the step (1), the method for preparing the vetiver essential oil is as follows: crushing vetiver, adding a NaCl solution with the mass fraction of 3%, distilling and condensing to obtain an oil-water mixture, standing the oil-water mixture, separating an oil phase, and drying to obtain vetiver essential oil;
the mass ratio of the crushed vetiver to the NaCl solution is 1:15;
the distillation temperature is 100-105 ℃, and the distillation time is 3 hours.
7. The method for preparing the jerusalem artichoke flower compound solid essential oil for assisting in improving memory as claimed in claim 4, wherein in the step (1), the method for preparing the basil essential oil is as follows: pulverizing herba Ocimi, adding 3% NaCl solution, distilling, condensing to obtain oil-water mixture, standing, separating oil phase, and drying to obtain herba Ocimi essential oil;
the mass ratio of the crushed basil to the NaCl solution is 1:15;
the distillation temperature is 100-105 ℃, and the distillation time is 3 hours.
8. The method for preparing the jerusalem artichoke flower compound solid essential oil for assisting in improving memory as claimed in claim 4, wherein in the step (1), the method for preparing the jasmine essential oil is as follows: crushing jasmine, adding a NaCl solution with the mass fraction of 2%, distilling and condensing to obtain an oil-water mixture, standing the oil-water mixture, separating an oil phase, and drying to obtain jasmine essential oil;
the mass ratio of the crushed jasmine to the NaCl solution is 1:20;
the distillation temperature is 105-110 ℃, and the distillation time is 2h.
9. The method for preparing the jerusalem artichoke flower compound solid essential oil for assisting in improving memory as claimed in claim 4, wherein in the step (1), the method for preparing the jasmine essential oil is as follows: pulverizing laurel, adding 4% NaCl solution, distilling, condensing to obtain oil-water mixture, standing, separating oil phase, and drying to obtain laurel essential oil;
the mass ratio of the crushed laurel to the NaCl solution is 1:15;
the distillation temperature is 100-105 ℃, and the distillation time is 3 hours.
CN202310019554.5A 2023-01-06 2023-01-06 Jerusalem artichoke flower compound solid essential oil for assisting in improving memory and preparation method thereof Pending CN116270906A (en)

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