CN116267995A - Sterilization composition for preventing and treating wheat scab and application thereof - Google Patents
Sterilization composition for preventing and treating wheat scab and application thereof Download PDFInfo
- Publication number
- CN116267995A CN116267995A CN202310195992.7A CN202310195992A CN116267995A CN 116267995 A CN116267995 A CN 116267995A CN 202310195992 A CN202310195992 A CN 202310195992A CN 116267995 A CN116267995 A CN 116267995A
- Authority
- CN
- China
- Prior art keywords
- whig
- bacillus amyloliquefaciens
- prothioconazole
- wheat scab
- bactericidal composition
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241000209140 Triticum Species 0.000 title claims abstract description 40
- 235000021307 Triticum Nutrition 0.000 title claims abstract description 40
- 206010039509 Scab Diseases 0.000 title claims abstract description 30
- 239000000203 mixture Substances 0.000 title claims abstract description 25
- 230000001954 sterilising effect Effects 0.000 title description 3
- 238000004659 sterilization and disinfection Methods 0.000 title description 3
- 241000193744 Bacillus amyloliquefaciens Species 0.000 claims abstract description 28
- 201000010099 disease Diseases 0.000 claims abstract description 27
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims abstract description 27
- MNHVNIJQQRJYDH-UHFFFAOYSA-N 2-[2-(1-chlorocyclopropyl)-3-(2-chlorophenyl)-2-hydroxypropyl]-1,2-dihydro-1,2,4-triazole-3-thione Chemical compound N1=CNC(=S)N1CC(C1(Cl)CC1)(O)CC1=CC=CC=C1Cl MNHVNIJQQRJYDH-UHFFFAOYSA-N 0.000 claims abstract description 25
- 239000005825 Prothioconazole Substances 0.000 claims abstract description 25
- 230000000844 anti-bacterial effect Effects 0.000 claims abstract description 19
- 239000002068 microbial inoculum Substances 0.000 claims abstract description 12
- 230000001580 bacterial effect Effects 0.000 claims description 11
- 239000000725 suspension Substances 0.000 claims description 10
- 241000894006 Bacteria Species 0.000 claims description 9
- 239000000575 pesticide Substances 0.000 claims description 9
- 239000003795 chemical substances by application Substances 0.000 claims description 7
- 238000009629 microbiological culture Methods 0.000 claims description 3
- 244000005700 microbiome Species 0.000 claims description 3
- 238000004321 preservation Methods 0.000 claims description 3
- 239000000839 emulsion Substances 0.000 claims description 2
- 239000004562 water dispersible granule Substances 0.000 claims description 2
- 239000004563 wettable powder Substances 0.000 claims description 2
- 230000000855 fungicidal effect Effects 0.000 claims 2
- 239000003090 pesticide formulation Substances 0.000 claims 2
- 239000002671 adjuvant Substances 0.000 claims 1
- 230000000813 microbial effect Effects 0.000 claims 1
- 239000012053 oil suspension Substances 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 15
- 239000000126 substance Substances 0.000 abstract description 9
- 239000003899 bactericide agent Substances 0.000 abstract description 8
- 239000013043 chemical agent Substances 0.000 abstract description 8
- 230000002195 synergetic effect Effects 0.000 abstract description 7
- 206010059866 Drug resistance Diseases 0.000 abstract description 4
- 238000011217 control strategy Methods 0.000 abstract description 4
- 244000052616 bacterial pathogen Species 0.000 abstract description 3
- 241000233866 Fungi Species 0.000 abstract description 2
- 239000002054 inoculum Substances 0.000 abstract 1
- 239000003814 drug Substances 0.000 description 10
- 241000196324 Embryophyta Species 0.000 description 8
- 238000012360 testing method Methods 0.000 description 8
- 230000002265 prevention Effects 0.000 description 6
- 241000223218 Fusarium Species 0.000 description 5
- 239000007788 liquid Substances 0.000 description 5
- 241000223195 Fusarium graminearum Species 0.000 description 4
- 150000001875 compounds Chemical class 0.000 description 4
- 230000005764 inhibitory process Effects 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 235000013305 food Nutrition 0.000 description 3
- 230000001965 increasing effect Effects 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- 241000223600 Alternaria Species 0.000 description 2
- 239000001888 Peptone Substances 0.000 description 2
- 108010080698 Peptones Proteins 0.000 description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 2
- 229930006000 Sucrose Natural products 0.000 description 2
- 230000000996 additive effect Effects 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 235000015278 beef Nutrition 0.000 description 2
- 230000000443 biocontrol Effects 0.000 description 2
- 229940041514 candida albicans extract Drugs 0.000 description 2
- 235000013339 cereals Nutrition 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 230000003111 delayed effect Effects 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 210000005069 ears Anatomy 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 238000011835 investigation Methods 0.000 description 2
- 235000019319 peptone Nutrition 0.000 description 2
- 239000005720 sucrose Substances 0.000 description 2
- 239000000375 suspending agent Substances 0.000 description 2
- 238000010998 test method Methods 0.000 description 2
- 231100000820 toxicity test Toxicity 0.000 description 2
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 2
- 230000001018 virulence Effects 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 239000012138 yeast extract Substances 0.000 description 2
- PXMNMQRDXWABCY-UHFFFAOYSA-N 1-(4-chlorophenyl)-4,4-dimethyl-3-(1H-1,2,4-triazol-1-ylmethyl)pentan-3-ol Chemical compound C1=NC=NN1CC(O)(C(C)(C)C)CCC1=CC=C(Cl)C=C1 PXMNMQRDXWABCY-UHFFFAOYSA-N 0.000 description 1
- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 description 1
- 244000063299 Bacillus subtilis Species 0.000 description 1
- 235000014469 Bacillus subtilis Nutrition 0.000 description 1
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 description 1
- 239000005502 Cyhalofop-butyl Substances 0.000 description 1
- TYIYMOAHACZAMQ-CQSZACIVSA-N Cyhalofop-butyl Chemical group C1=CC(O[C@H](C)C(=O)OCCCC)=CC=C1OC1=CC=C(C#N)C=C1F TYIYMOAHACZAMQ-CQSZACIVSA-N 0.000 description 1
- 241000221785 Erysiphales Species 0.000 description 1
- 239000005788 Fluxapyroxad Substances 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 208000031888 Mycoses Diseases 0.000 description 1
- 231100000678 Mycotoxin Toxicity 0.000 description 1
- 241001024327 Oenanthe <Aves> Species 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 206010034133 Pathogen resistance Diseases 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 229930182558 Sterol Natural products 0.000 description 1
- 239000005839 Tebuconazole Substances 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000008485 antagonism Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000006013 carbendazim Substances 0.000 description 1
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000017858 demethylation Effects 0.000 description 1
- 238000010520 demethylation reaction Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000000857 drug effect Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- SXSGXWCSHSVPGB-UHFFFAOYSA-N fluxapyroxad Chemical compound FC(F)C1=NN(C)C=C1C(=O)NC1=CC=CC=C1C1=CC(F)=C(F)C(F)=C1 SXSGXWCSHSVPGB-UHFFFAOYSA-N 0.000 description 1
- 230000009036 growth inhibition Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 239000002636 mycotoxin Substances 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 239000000447 pesticide residue Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 230000008261 resistance mechanism Effects 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 150000003432 sterols Chemical class 0.000 description 1
- 235000003702 sterols Nutrition 0.000 description 1
- 150000003852 triazoles Chemical class 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/22—Bacillus
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/64—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with three nitrogen atoms as the only ring hetero atoms
- A01N43/647—Triazoles; Hydrogenated triazoles
- A01N43/653—1,2,4-Triazoles; Hydrogenated 1,2,4-triazoles
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P3/00—Fungicides
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The invention provides a bactericidal composition for preventing and treating wheat scab and application thereof, belongs to the field of agricultural bactericides, and particularly relates to a bactericidal composition containing bacillus amyloliquefaciens WHIG (white fungus) inoculant and prothioconazole and application thereof in preventing and treating wheat scab; the mass ratio of the bacillus amyloliquefaciens WHIG microbial inoculum to the prothioconazole is 80:1-1:8. The invention utilizes the biological-chemical cooperative control strategy, integrates the advantages of the biological control microbial inoculum and the chemical bactericide, can reduce the application amount of the chemical agent, improve the stability of biological control, delay the generation of drug resistance of pathogenic bacteria to the chemical agent, achieve the effect of effectively controlling diseases, and has obvious synergistic effect.
Description
Technical Field
The invention belongs to the field of agricultural bactericides, and particularly relates to a bactericidal composition for preventing and treating wheat scab and application thereof.
Background
Wheat Scab (Fusarium head blight, FHB or Scab for short) is a fungal disease with strong hazard mainly caused by fusarium graminearum (Fusarium graminearum), is the most common epidemic disease in wheat producing areas in China and even worldwide, and becomes one of the most important diseases which currently restrict the wheat production safety and the wheat food quality safety in China. The disease not only causes serious loss of wheat yield, but also worsens quality of various mycotoxins contained in the disease-sensitive grains, seriously influences food safety and endangers human and animal health.
Because the wheat scab resistant germplasm resources are limited, the resistance mechanism is complex, and the variety with high scab resistance is not obtained in production, the demand of the resistant variety in production cannot be met. At present, the main measure for preventing and controlling the gibberellic disease is to carry out medicament prevention and control in the period from the wheat snapping stage to the flowering stage. Because the wheat scab pathogenic bacteria group has resistance to traditional medicaments such as carbendazim, compound preparations thereof and the like, the problem of prevention effect reduction and even prevention failure is caused, a batch of bactericides with different action mechanisms such as tebuconazole, cyhalofop-butyl, prothioconazole, fluxapyroxad and the like are developed on the market.
The perennial use of the chemical agent can cause the gibberella wheat germ to generate drug resistance to the bactericide, so that the control effect of the chemical agent is reduced, and even the control fails. In order to improve the control effect, the usage amount of chemical pesticides is increased, so that not only can chemical pesticide residues be accumulated to influence the health of human bodies, but also ecological balance can be destroyed to cause environmental pollution. With the increasing importance of people on the problems of environmental protection, ecological balance, food safety and the like, the research and development of a technology for comprehensively preventing and treating wheat scab mainly by using efficient, safe and nuisance-free microorganism compound pesticides is urgent. Biological control is safe to people and livestock, has the advantages of being green, environment-friendly, long in acting time, not easy to generate drug resistance of bacteria and the like, can reduce the use of chemical pesticides, improve the quality, and can also reduce the pollution to ecology and environment, and is increasingly popular as an environment-friendly control technology.
Bacillus amyloliquefaciens is a bacterium with high relativity with bacillus subtilis and is widely distributed in nature. The bacillus amyloliquefaciens can produce a series of metabolites with the activity of widely inhibiting fungi and bacteria in the growth process, has wide application prospect in the aspect of biological control, and gradually becomes a microorganism with the development potential of biological pesticides. The bacillus amyloliquefaciens WHIG is obtained by separating rice disease control subject components of the institute of plant protection and agricultural product quality safety of Anhui province, has broad-spectrum antibacterial activity and has good inhibition effect on various pathogenic bacteria. The strain is preserved in China general microbiological culture Collection center (China Committee for culture Collection) for 15 days 04 month 2015, and has an address of No.1, no. 3, and a preservation number of CGMCC No.10722. The strain is named WHIG, and the strain is named in a classification mode: bacillus amyloliquefaciens (cf. CN 107136120A).
The prothioconazole is a sterol demethylation inhibitor (DMIs) bactericide developed by Bayer crop science company, is mainly used for preventing and treating various diseases on grains and beans, and has the advantages of wide sterilization range, good prevention and treatment effect, obvious yield increasing effect and the like compared with the traditional triazole bactericide. In China, prothioconazole was registered in 2019 for prevention and control of wheat scab, powdery mildew and rust. The prothioconazole serving as a novel chemical component has good control effect on wheat scab, but has great resistance risk after long-term independent use.
The control strategy combining biological control and chemical control is utilized, so that the defects of slow drug effect, poor control effect and poor stability of biological control can be overcome, the application amount of chemical agents can be reduced, the effect of effectively controlling diseases can be achieved, and no related report on the combined use of bacillus amyloliquefaciens WHIG and prothioconazole exists in the prior art. The applicant researches find that the combination of bacillus amyloliquefaciens WHIG and prothioconazole has a good synergistic effect on wheat scab.
Disclosure of Invention
The invention utilizes the control strategy of combining biological control and chemical control, not only can reduce the application amount of chemical agents, but also can improve the stability of biological control, thereby achieving the aim of effectively controlling diseases, and the composition shows remarkable synergy.
The technical scheme of the invention is as follows:
the bactericidal composition for preventing and treating wheat scab is characterized by comprising bacillus amyloliquefaciens WHIG microbial inoculum and prothioconazole; the mass ratio of the bacillus amyloliquefaciens WHIG microbial inoculum to the prothioconazole is 80:1-1:8; the bacillus amyloliquefaciens WHIG is preserved in the China general microbiological culture Collection center (China Committee for culture Collection) with the address of 1 # 3 of North West Lu of the Korean area of Beijing city and the preservation number of 10722; bacillus amyloliquefaciens WHIG microbial inoculum concentration 1×10 6 -1×10 10 cfu/mL。
Preferably, the concentration of the bacillus amyloliquefaciens WHIG microbial inoculum is 1 multiplied by 10 7 -1×10 9 cfu/mL; the mass ratio of the bacillus amyloliquefaciens WHIG microbial inoculum to the prothioconazole is 50:1-5:1. Further preferably, the concentration of the bacillus amyloliquefaciens WHIG microbial inoculum is 1×10 9 cfu/mL; the mass ratio of the bacillus amyloliquefaciens WHIG microbial inoculum to the prothioconazole is 50:1-5:1.
The bactericidal composition also comprises pesticide auxiliary ingredients, and is prepared into a pesticide preparation. The pesticide preparation is suspending agent, wettable powder, water dispersible granule, aqueous emulsion and dispersible oil suspending agent. The auxiliary materials are prepared by adopting a conventional preparation method by selecting conventional auxiliary material components in the field.
The bactericidal composition can be used for preventing and controlling crop diseases, and the crop diseases are wheat scab.
By adopting the technical scheme, the invention has the following beneficial effects:
the invention utilizes the control strategy of combining biological control and chemical control, not only can reduce the application amount of chemical agents, but also can improve the stability of biological control, thereby achieving the effect of effectively controlling diseases.
When the mass ratio of the WH1G bacterial suspension to the prothioconazole is 50:1-5:1, the composition has obvious synergistic effect on wheat scab, so that the dosage of chemical agents can be effectively reduced, the generation of pathogen resistance can be delayed, and the wheat scab can be effectively prevented and treated.
Detailed Description
The following is a further explanation of the embodiments of the present invention in connection with specific examples.
Example 1: indoor toxicity test of compound medicament on wheat scab
1.1 test materials
Biocontrol bacteria: bacillus amyloliquefaciens WH1G, institute of plant protection and agricultural product quality safety at the national academy of agricultural sciences, anhui province. Culturing in modified NA culture medium (beef extract 1.0g, peptone 5.0g, yeast extract 5.0g, naCl5.0g, sucrose 10.0g, agar 20.0g, distilled water 1000mL, pH 6.8-7.0) at 28deg.C. Preparing Bacillus amyloliquefaciens WH1G bacterial suspension with concentration of 1×10 9 cfu/mL。
The wheat gibberella (Fusarium graminearum) is separated, screened, identified and stored by the institute of plant protection and agricultural product quality safety, national academy of agricultural sciences, anhui province.
Prothioconazole, commercially available.
1.2 test methods
The hypha growth rate method is adopted. On the basis of pre-experiments, PDA plates containing medicaments with different gradient concentrations are prepared, activated wheat gibberella bacteria blocks (with the diameter of 5 mm) are placed in the center of the medicament-containing plates, and the plates are placed in a 28 ℃ incubator for culture. And controls were set, and each treatment was repeated 4 times. When the control colony grows to be 2/3 of the culture dish, measuring the diameter of each treated colony by adopting a crisscross method, and calculating the average inhibition rate.
Average inhibition ratio = [ (average control colony diameter-average treatment colony diameter)/(average control colony diameter-5.0) ]×100%.
Converting the mycelium growth inhibition rate into inhibition rate value (y), converting the medicine concentration into concentration logarithm (x), and concentratingThe logarithmic scale is the abscissa and the probability value is the ordinate, the virulence regression line is calculated, the virulence regression equation of single agent and mixed agent for the pathogen is calculated, and EC is calculated 50 Values and correlation coefficient r values.
The synergy coefficient (SR) of the mixture is calculated according to the Wadley method, so that the synergy of the mixed medicament is evaluated, namely SR > 1.5 is the synergy, SR < 0.5 is the antagonism, SR < 0.5 is less than or equal to 1.5 is the additive effect. The synergy coefficient (SR) is calculated as follows:
wherein: EC of X-ray mixture 50 Theoretical values in μg/mL;
P A the percentage of WH1G in the mixture, expressed as a percentage (%);
P B the percentage content of prothioconazole in the mixture, expressed as percentage (%);
EC of WH1G in A-mix 50 Values in μg/mL;
EC of prothioconazole in B-mixture 50 Values in μg/mL;
the synergistic coefficient of the SR-blend;
EC of X1-mixture 50 Measured values are given in μg/mL.
1.3 test results
Test of EC of WH1G bacterial suspension to Alternaria wheat 50 A value of 8.5494mg/L; EC of prothioconazole against Alternaria wheat germ 50 The value was 1.1016mg/L. When the mass ratio of the WH1G bacterial suspension to the prothioconazole is 50:1-5:1, the synergistic effect is shown on wheat scab bacteria, and the additive effect is shown under other ratio conditions. When the composition shows synergistic effect, the dosage of single agent can be effectively reduced, and the generation of drug resistance can be delayed.
Table 1 indoor toxicity test of formulated agent on wheat scab
Remarks: 1mL in the bacterial suspension configuration was calculated as 1 g.
Example 2: field efficacy test of compound medicament on wheat scab
2.1 test materials
Biocontrol bacteria: bacillus amyloliquefaciens WH1G, institute of plant protection and agricultural product quality safety at the national academy of agricultural sciences, anhui province. Culturing in modified NA culture medium (beef extract 1.0g, peptone 5.0g, yeast extract 5.0g, naCl5.0g, sucrose 10.0g, agar 20.0g, distilled water 1000mL, pH 6.8-7.0) at 28deg.C. Preparing Bacillus amyloliquefaciens WH1G bacterial suspension with bacterial content of 1×10 9 cfu/mL。
Gibberella wheat germ (Fusarium graminearum): separated, screened, identified and stored by the institute of plant protection and agricultural product quality safety of the national academy of agricultural science of Anhui province.
Wheat scab spore liquid: prepared by the institute of plant protection and agricultural product quality safety of the national academy of agricultural sciences of Anhui province, the concentration of spore liquid is 1×10 6 The inoculation amount of spore liquid is 450L/hm 2 。
Prothioconazole, commercially available.
2.2 test methods
And (3) manually inoculating the wheat with the gibberellic disease spore liquid at the early stage of flowering. The agents with different concentrations are uniformly sprayed on the wheat ears respectively 24 hours after inoculation. A blank control was set. Each treatment was repeated 3 times, 4 square meters per cell. Investigation was started 25d after administration. Each repeated diagonal line is sampled at 5 points, 100 ears are investigated at each point, the number of disease stages and the total ears are recorded at each point, and the grading standard is referred to the pesticide field efficacy test criterion of the agricultural industry standard: the bactericide is used for preventing and treating wheat scab (NY/T1464.14-2007) and is evaluated by the ratio (S) of the area of the disease spike to the area of the whole spike.
Disease severity grading criteria:
level 0: the whole plant is free from diseases;
stage 1: s is less than 1/4;
3 stages: s is more than or equal to 1/4 and less than 1/2;
5 stages: s is more than or equal to 1/2 and less than 3/4;
7 stages: s is more than or equal to 3/4.
Disease index = Σ [ (number of disease plants at each stage×number of relative disease stages)/(total number of investigation×number of highest disease stages) ]100
Control effect (%) = [ (disease index of blank control area-disease index of treatment area)/disease index of blank control area ] ×100
2.3 test results
As shown in table 1, the control effect of the WH1G bacterial suspension alone on wheat scab is; the prevention and treatment effect of the prothioconazole on wheat scab is percent. After the WH1G bacterial suspension and prothioconazole are combined, the control effect of the medicament on wheat scab can be obviously improved, and the synergistic effect is obvious.
Table 2 field test of formulated agent on wheat scab
Remarks: the spraying liquid medicine amount per cell is 300mL, 1mL in the bacterial suspension configuration is calculated according to 1g,
finally, it should also be noted that the above list is merely a few specific embodiments of the present invention. Obviously, the invention is not limited to the above embodiments, but many variations are possible. All modifications which can be directly derived or suggested by the person skilled in the art from the teaching of the present disclosure are considered to be within the scope of the present invention.
Claims (7)
1. The bactericidal composition for preventing and treating wheat scab and the application thereof are characterized by comprising bacillus amyloliquefaciens WHIG bacterial agent and prothioconazole; the mass ratio of the bacillus amyloliquefaciens WHIG microbial inoculum to the prothioconazole is 80:1-1:8; wherein the bacillus amyloliquefaciens WHIG is preserved in China general microbiological culture Collection center (China Committee for culture Collection of microorganisms) for 15 days in 04 month 2015, and has an address of 1 # 3 of North West Lu of the Korean area of Beijing city and a preservation number of 10722; concentration of bacillus amyloliquefaciens WHIG microbial inoculumIs 1X 10 6 -1×10 10 cfu／mL。
2. The bactericidal composition of claim 1, wherein the concentration of bacillus amyloliquefaciens WHIG bacteria is 1 x 10 7 -1×10 9 cfu/mL; the mass ratio of the bacillus amyloliquefaciens WHIG microbial inoculum to the prothioconazole is 50:1-5:1.
3. The bactericidal composition of claim 2, wherein the concentration of bacillus amyloliquefaciens WHIG bacteria is 1 x 10 9 cfu/mL; the mass ratio of the bacillus amyloliquefaciens WHIG microbial inoculum to the prothioconazole is 50:1-5:1.
4. A fungicidal composition according to any one of claims 1-3, further comprising an adjuvant component of a pesticide, prepared as a pesticide formulation.
5. The bactericidal composition of claim 4, wherein the pesticide formulation is a suspension, a wettable powder, a water dispersible granule, an aqueous emulsion, a dispersible oil suspension.
6. Use of the fungicidal composition according to any one of claims 1 to 5 for controlling crop diseases.
7. The use according to claim 5, wherein the crop disease is wheat scab.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310195992.7A CN116267995B (en) | 2023-02-28 | 2023-02-28 | Sterilization composition for preventing and treating wheat scab and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310195992.7A CN116267995B (en) | 2023-02-28 | 2023-02-28 | Sterilization composition for preventing and treating wheat scab and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN116267995A true CN116267995A (en) | 2023-06-23 |
| CN116267995B CN116267995B (en) | 2023-11-03 |
Family
ID=86814413
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310195992.7A Active CN116267995B (en) | 2023-02-28 | 2023-02-28 | Sterilization composition for preventing and treating wheat scab and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN116267995B (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104673705A (en) * | 2014-07-06 | 2015-06-03 | 华中农业大学 | Bacillus amyloliquefaciens capable of preventing and treating wheat scab and application thereof |
| CN104762223A (en) * | 2014-11-06 | 2015-07-08 | 河北省科学院生物研究所 | Bacillus amyloliquefaciense BA-KA3 and application thereof |
| CN105918336A (en) * | 2016-07-12 | 2016-09-07 | 安徽省农业科学院植物保护与农产品质量安全研究所 | Bactericidal composition containing prothioconazole and prothioconazole |
| CN107041384A (en) * | 2017-05-19 | 2017-08-15 | 安徽省农业科学院植物保护与农产品质量安全研究所 | A kind of bactericidal composition and its application containing bacillus amyloliquefaciens and Fluoxastrobin |
| CN107090419A (en) * | 2017-05-24 | 2017-08-25 | 浙江大学 | Bacillus amyloliquefaciens and its application |
| CN107660557A (en) * | 2017-05-19 | 2018-02-06 | 安徽省农业科学院植物保护与农产品质量安全研究所 | A kind of bactericidal composition containing bacillus amyloliquefaciens and Difenoconazole |
| CN114410530A (en) * | 2022-01-27 | 2022-04-29 | 福建农林大学 | Bacillus amyloliquefaciens W0101 and application thereof |
-
2023
- 2023-02-28 CN CN202310195992.7A patent/CN116267995B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104673705A (en) * | 2014-07-06 | 2015-06-03 | 华中农业大学 | Bacillus amyloliquefaciens capable of preventing and treating wheat scab and application thereof |
| CN104762223A (en) * | 2014-11-06 | 2015-07-08 | 河北省科学院生物研究所 | Bacillus amyloliquefaciense BA-KA3 and application thereof |
| CN105918336A (en) * | 2016-07-12 | 2016-09-07 | 安徽省农业科学院植物保护与农产品质量安全研究所 | Bactericidal composition containing prothioconazole and prothioconazole |
| CN107041384A (en) * | 2017-05-19 | 2017-08-15 | 安徽省农业科学院植物保护与农产品质量安全研究所 | A kind of bactericidal composition and its application containing bacillus amyloliquefaciens and Fluoxastrobin |
| CN107660557A (en) * | 2017-05-19 | 2018-02-06 | 安徽省农业科学院植物保护与农产品质量安全研究所 | A kind of bactericidal composition containing bacillus amyloliquefaciens and Difenoconazole |
| CN107090419A (en) * | 2017-05-24 | 2017-08-25 | 浙江大学 | Bacillus amyloliquefaciens and its application |
| CN114410530A (en) * | 2022-01-27 | 2022-04-29 | 福建农林大学 | Bacillus amyloliquefaciens W0101 and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN116267995B (en) | 2023-11-03 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EP3398437B1 (en) | Metconazole-containing pesticide composition for preventing and treating fusarium head blight and application thereof | |
| CA2985520C (en) | A pesticide composition comprising tebuconazole and jinggangmycin for preventing and controlling fusarium head blight, and a method wherein the pesticide composition is applied to cultivated grain crops | |
| CN104877932A (en) | Paenibacillus polymyxa and application thereof | |
| CN110713951A (en) | Pantoea agglomerans strain and application thereof | |
| CN107041384B (en) | Bactericidal composition containing bacillus amyloliquefaciens and azoxystrobin and application thereof | |
| CN107821439B (en) | Composition for preventing and treating rice sheath blight disease and preparation method thereof | |
| CN112602708A (en) | Pesticide composition and application thereof | |
| CN116267995B (en) | Sterilization composition for preventing and treating wheat scab and application thereof | |
| CN109452290B (en) | Composite bactericide containing benziothiazolinone and nisin and application thereof | |
| CN104705324B (en) | A kind of bactericidal composition containing Zhongshengmycin and chitosan oligosaccharide | |
| CN112400901B (en) | Bactericidal composition and application thereof | |
| CN101755761A (en) | Fluquinconazole-based synergistic pesticide composition | |
| CN115363043A (en) | Composition for preventing and treating alternaria alternate, preparation method and application | |
| CN113575602A (en) | Powdery mildew resistant bactericidal active agent | |
| CN116114712B (en) | Bactericidal composition containing bacillus amyloliquefaciens and application thereof | |
| CN112772658A (en) | Bactericidal composition of spirosporium roseum spores and boscalid and application of bactericidal composition | |
| CN110862948B (en) | Bacillus amyloliquefaciens and application thereof in preventing and controlling false smut of rice | |
| CN114304173B (en) | Pesticide composition for preventing and treating sclerotinia rot and application thereof | |
| CN114525227B (en) | Microbial agent containing bacillus bailii CY30 and application thereof | |
| CN115777722B (en) | Application of alexidine dihydrochloride in inhibiting plant pathogenic bacteria | |
| CN115624030B (en) | Sterilization composition containing fluxapyroxad hydroxylamine | |
| CN110583669B (en) | Bactericidal composition for melon diseases | |
| CN110915816B (en) | Method for preventing and treating false smut of rice | |
| CN105724394A (en) | Epoxiconazole-containing pesticide composition used for preventing and treating wheat head blight and application thereof | |
| CN113207889A (en) | Bactericidal composition containing ethylicin and tebuconazole and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |