CN116253806A - Polygonatum sibiricum levan, pharmaceutical composition and application - Google Patents

Polygonatum sibiricum levan, pharmaceutical composition and application Download PDF

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CN116253806A
CN116253806A CN202310056112.8A CN202310056112A CN116253806A CN 116253806 A CN116253806 A CN 116253806A CN 202310056112 A CN202310056112 A CN 202310056112A CN 116253806 A CN116253806 A CN 116253806A
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levan
polygonatum
ethanol
polygonatum sibiricum
supernatant
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彭腾
吴明一
张军银
罗兰
周志鹏
杨莲
陈胡兰
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Chengdu University of Traditional Chinese Medicine
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    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
    • C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
    • C08B37/0051—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid beta-D-Fructofuranans, e.g. beta-2,6-D-fructofuranan, i.e. levan; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/125—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives containing carbohydrate syrups; containing sugars; containing sugar alcohols; containing starch hydrolysates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
    • A61K31/733—Fructosans, e.g. inulin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/14—Prodigestives, e.g. acids, enzymes, appetite stimulants, antidyspeptics, tonics, antiflatulents
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
    • C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Abstract

The invention relates to a polygonatum sibiricum levan, a pharmaceutical composition and application, wherein the polygonatum sibiricum levan is prepared by the following method: s1, extracting crude polysaccharide: weighing Polygonatum cyrtonema Fabricius, adding water, extracting under reflux, mixing the extractive solutions, and centrifuging to obtain supernatant; concentrating, adding ethanol to obtain 40% ethanol precipitate and supernatant; adding ethanol to obtain 60% ethanol sediment and supernatant; continuously adding ethanol to obtain 80% ethanol sediment, washing with ethanol, and volatilizing alcohol to obtain crude rhizoma Polygonati levan; s2, purifying: dissolving the crude sugar in deionized water, loading on DEAE-52 weak anion exchange column, dissolving with deionized water and 0.1, 0.5 and 1.0M sodium chlorideEluting the liquid, analyzing the eluted fraction by a chromatograph, collecting fractions containing levan, concentrating under reduced pressure, and freeze-drying to obtain purified polygonatum levan; has a structure represented by the following formula I, m, n and p represent the number of sugar repeating units, m, n and p are natural numbers, and m+n>p; the weight average molecular weight of the polygonatum sibiricum levan is 2000 Da-8000 Da, and the polydispersity coefficient is 1-3.
Figure DDA0004060573940000011

Description

Polygonatum sibiricum levan, pharmaceutical composition and application
The present patent application is a divisional application of Chinese patent application with application number 202110255156.4, and the patent name is "Polygonatum sibiricum fructan and preparation method and application thereof" (application date: 2021, 09, year 03), the contents of which are incorporated herein by reference in their entirety.
Technical Field
The invention relates to the technical field of foods and medicines, in particular to a polygonatum sibiricum levan and a preparation method and application thereof.
Background
Rhizoma Polygonati is a plant belonging to genus Polygonatum of family Liliaceae, and is named for its tonic effect. The plant mainly comprises rhizoma Polygonati (Polygonatum sibiricum), rhizoma Polygonati Odorati (Polygonatum kingianum), rhizoma Polygonati cyrtomii Falcati (Polygonatum cyrtonema), rhizoma Polygonati Odorati (Polygonatum cathcartii), rhizoma Polygonati Odorati (polygonatum odoratum), rhizoma Polygonati Odorati (Polygonatum prattii), rhizoma Polygonati Odorati (Polygonatum humile), mao Tongyu bamboo (Polygonatum inflatum), rhizoma Polygonati Odorati (Polygonatum prattii) rhizoma Polygonati Odorati (Polygonatum sibiricum), rhizoma Polygonati (Polygonatum desoulayi), rhizoma Polygonati Odorati (Polygonatum desoulayi), rhizoma Polygonati (Polygonatum desoulayi) rhizoma Polygonati (Polygonatum desoulayi), rhizoma Polygonati (Polygonatum desoulayi) rhizoma Polygonati of Polygonatum (Polygonatum desoulayi), rhizoma Polygonati of Pacific leaf (Polygonatum desoulayi), rhizoma Polygonati of Hubei (Polygonatum desoulayi), rhizoma Polygonati of Grifolii (Polygonatum desoulayi) rhizoma Polygonati of root of festival (Polygonatum desoulayi), rhizoma Polygonati of opposite leaf (Polygonatum desoulayi) rhizoma Polygonati in Hubei (Polygonatum desoulayi), rhizoma Polygonati in Grace (Polygonatum desoulayi). Rhizoma Polygonati medicinal part is rhizome, sweet and flat. Tonifying qi and yin, strengthening spleen, moistening lung and tonifying kidney. Can be used for treating spleen deficiency, gastric weakness, tiredness, debilitation, dry mouth, anorexia, lung deficiency, cough, essence and blood deficiency, internal heat, and diabetes. The rhizome of Polygonatum plant can be used as medicine, and the chemical components in the rhizome mainly comprise: the polygonatum polysaccharide is the most medicinal component in the polygonatum plant, and comprises polygonatum polysaccharide, mucin, starch, steroid saponin, anthraquinone compounds, alkaloids, cardiac glycoside, lignans, vitamins, fat, protein and a plurality of amino acids which are useful for human bodies.
However, the chemical structure of plant polysaccharide is extremely complex, and the same plant often contains a plurality of different types of polysaccharide, and the research on the biological efficacy of polysaccharide is less developed, so that the application development of plant polysaccharide in the fields of biological medicine and food is slow.
Disclosure of Invention
The invention aims at: aiming at the problems of insufficient research depth of rhizoma polygonati, complex plant polysaccharide components in rhizoma polygonati and slow research progress of plant polysaccharide in the prior art, the invention provides a rhizoma polygonati levan, a preparation method and application thereof.
In order to achieve the above purpose, the technical scheme adopted by the invention is as follows:
a polygonatum levan, which has a structure represented by the following general formula I:
Figure BDA0004060573920000021
in the formula (I), m, n and p represent the number of sugar repeating units, m, n and p are natural numbers including 0, and m+n > p.
The novel-structure polygonatum levan disclosed by the invention can promote the proliferation of probiotics including lactobacillus and bifidobacterium through pharmacological research, and has remarkable effect in 2-12 hours; along with the increase of the drug concentration, the capacity of the polygonatum levan for promoting the proliferation of probiotics is obviously enhanced, which indicates that the polygonatum levan can promote the growth of the probiotics.
As a preferable scheme of the invention, the weight average molecular weight of the polygonatum fructan is 2000 Da-8000 Da, and the polydispersity coefficient is 1-3.
In order to better obtain the high-purity product of the polygonatum levan, the invention also provides a preparation method for ensuring better separation effect in the polygonatum extraction process, and the purity of the obtained product meets application requirements.
The preparation method of the polygonatum sibiricum levan comprises the following steps:
extracting crude rhizome polysaccharide of the Polygonatum plant;
purifying the components with the weight average molecular weight of 2000Da to 8000Da in the rhizoma polygonati rhizome crude polysaccharide to obtain the rhizoma polygonati levan.
Further, the step of extracting the polygonatum fructan comprises the following steps: extracting rhizome of Polygonatum plant with hot water, respectively precipitating Polygonatum polysaccharide with 95% ethanol to reach ethanol concentration of 40v%, 60v% and 80v%, centrifuging, and collecting precipitate with 80v% ethanol concentration. Gradually increasing the concentration of ethanol according to the fractional precipitation mode, and realizing the purposes of precipitation, separation and purification.
Further, the Polygonatum plant refers to Polygonatum species. Can be one or more of rhizoma Polygonati belonging to Polygonatum genus. The above Polygonatum species are at least one existing Polygonatum species.
Further, the preparation method comprises the following steps: s1, extracting crude polysaccharide of the rhizoma polygonati under the concentration of 80v% ethanol; s2, purifying the components with the weight average molecular weight of 2000 Da-8000 Da in the polygonatum polysaccharide to obtain the polygonatum levan.
Further, the crude polysaccharide with an ethanol concentration of 80v% is obtained by extracting rhizome of Polygonatum plant with hot water, and separating by ethanol precipitation to obtain precipitate with an ethanol concentration of 80 v%.
In one embodiment, the step of extracting the polygonatum fructan comprises the steps of: precipitating the rhizoma Polygonati with 95% ethanol to obtain ethanol solution with final ethanol concentration of 80%, centrifuging, and collecting ethanol precipitate.
In one embodiment, the method for purifying the components with the weight average molecular weight of 2000Da to 8000Da in the polygonatum polysaccharide is selected from one or more of gel exclusion chromatography, dialysis and ultrafiltration.
In one of the embodiments of the present invention, the Polygonatum plant comprises Polygonatum sibiricum, polygonatum kingianum, polygonatum cyrtonema, polygonatum odoratum, mao Tongyu bamboo rhizoma Polygonati Odorati, rhizoma Polygonati O one or more of rhizoma Polygonati, rhizoma Polygonati of Duhua, rhizoma Polygonati of Polygonatum, rhizoma Polygonati of Hubei, rhizoma Polygonati of Grifola, rhizoma Polygonati of Penthorn leaf, rhizoma Polygonati of Polygonatum sibiricum, rhizoma Polygonati of Sinkiang, rhizoma Polygonati of Polygonatum and rhizoma Polygonati of Polygonatum angustifolium.
A pharmaceutical composition comprising an effective amount of a polygalactan of Polygonatum sibiricum.
The application of the polygonatum sibiricum levan in the preparation of food and/or medicine for regulating gastrointestinal flora, promoting intestinal peristalsis and/or promoting digestion.
An application of the Polygonatum sibiricum levan in promoting the growth of probiotics is provided.
In one embodiment, the dosage form of the pharmaceutical composition is one of freeze-dried powder, oral liquid, capsules and the like.
The application of the pharmaceutical composition in preparing food and medicine for promoting digestion and intestinal peristalsis is provided.
The application of the Polygonatum sibiricum fructan in preparing facial masks, cosmetics, daily chemicals and the like.
In summary, due to the adoption of the technical scheme, the beneficial effects of the invention are as follows:
1. the polygonatum levan is obtained by first separation, has the effect of promoting the proliferation of probiotics, has obvious concentration dependence characteristic, and can be applied as an active ingredient of various preparations for promoting the probiotics.
2. The polygonatum sibiricum levan provided by the invention can promote proliferation of probiotics and regulate proliferation of intestinal probiotics, so that the polygonatum sibiricum levan has a dietetic therapy health care application value. Can be used as main or auxiliary active ingredients of food, health care products or medicines in the aspects of prevention, treatment or conditioning.
Description of the drawings:
FIG. 1 is an HPLC chart of Polygonatum sibiricum levan;
FIG. 2 is a MALDI-TOF MS diagram of Polygonatum sibiricum levan;
FIG. 3 is a diagram showing a monosaccharide composition analysis HPLC of Polygonatum sibiricum fructan, wherein FIG. a is a diagram showing a monosaccharide standard, FIG. b is a diagram showing Polygonatum sibiricum fructan, fru represents fructose, and Glc represents glucose;
FIG. 4 is an infrared spectrum of Polygonatum sibiricum levan;
FIG. 5 is a nuclear magnetic resonance spectrum of Polygonatum sibiricum levan, wherein FIG. a is a 1H NMR spectrum and FIG. b is a 13C NMR spectrum;
FIG. 6 is a nuclear magnetic resonance spectrum of Polygonatum sibiricum levan, wherein FIG. a is an HSQC-TOCSY NMR chart and FIG. b is an HMBC NMR chart;
fig. 7 is the effect of different times of polygonatum levan on the pH of the medium inoculated with probiotics including lactobacillus and bifidobacteria (n=3);
fig. 8 is the effect of different time-intervals of polygonatum levan on the OD values of the medium for inoculating probiotics including lactobacillus and bifidobacteria (n=3);
figure 9 shows the effect of Polygonatum sibiricum levan on bacterial numbers in the medium inoculated with probiotics including Lactobacillus and Bifidobacterium (n=3) at different times.
Detailed Description
The present invention will be described more fully hereinafter in order to facilitate an understanding of the present invention, and preferred embodiments of the present invention are set forth. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. The term "and/or" as used herein includes any and all combinations of one or more of the associated listed items.
The ethanol solution used in the present invention is an aqueous ethanol solution of volume percent, and the percentage is expressed in% and is understood to mean vol%.
The polygonatum sibiricum levan of one embodiment of the invention has a structure represented by the following general formula I:
Figure BDA0004060573920000051
in the formula (I), m, n and p represent the number of sugar repeating units, m, n and p are natural numbers including 0, and m+n > p.
The Polygonatum sibiricum fructan takes beta (2-6) glycosidic bond as main chain, and the side chain is fructose and is connected to the main chain by beta (2-1) glycosidic bond.
The inventor of the present invention has conducted intensive studies on Polygonatum sibiricum, and has surprisingly found that a novel structure of Polygonatum sibiricum fructan can be obtained by extraction and purification from Polygonatum plants such as Polygonatum cyrtonema, etc. using an innovative method. Further pharmacological activity research shows that the newly discovered polygonatum fructan can significantly promote the proliferation of probiotics, so that the polygonatum fructan has application in preparing intestinal tract regulating and digestion promoting medicines and/or foods.
Therefore, the polygonatum sibiricum levan with a novel structure, the preparation method and the medicinal composition thereof and the application in preparing medicines for intestinal tract regulation and digestion promotion are all reported for the first time.
In a specific example, the weight average molecular weight of the polygonatum fructan is 2000Da to 8000Da, and the polydispersity is 1 to 2. Preferably, the weight average molecular weight of the polygonatum fructan is 3000 Da-5000 Da.
In a specific example, when p=0 in the general formula I, to satisfy m+n > p, m and n have minimum values of m=n=1, and the polygalactan has a structure represented by the following chemical formula II:
Figure BDA0004060573920000052
the preparation method of the polygonatum sibiricum levan in an embodiment of the invention comprises the following steps S1-S2:
s1: extracting crude Polygonatum sibiricum fructan of Polygonatum plants.
S2: purifying the components with the weight average molecular weight of 2000Da to 8000Da in the crude polygonatum sibiricum levan to obtain the polygonatum sibiricum levan.
In one specific example of this embodiment, the method comprises, the Polygonatum plant includes, but is not limited to, polygonatum sibiricum, polygonatum cyrtonema, polygonatum odoratum, and Polygonatum odoratum Mao Tongyu bamboo, polygonatum sibiricum, polygonatum cyrtonema, polygonatum cy Mao Tongyu bamboo, polygonatum sibiricum, and Polygonatum sibiricum rhizoma Polygonati Odorati, rhizoma Polygonati. In view of the plant resource situation of the Polygonatum plant, preferably, the Polygonatum plant is one or more of Polygonatum sibiricum, polygonatum cyrtonema and Polygonatum odoratum.
As a preferred scheme of the invention, extracting the crude polysaccharide of the rhizoma polygonati under the concentration of 80v% ethanol specifically adopts the following method:
weighing rhizoma Polygonati (preferably rhizoma Polygonati, polygonatum cyrtonema Sieb) and extracting with 5-10 times of water under reflux for 1-4 times (each for 1-5 hr), filtering, mixing the extractive solutions, centrifuging to obtain supernatant, and concentrating to 0.5-3 times of the weight of the original rhizoma Polygonati. Then, the ethanol concentration was adjusted to 40v%, and the mixture was centrifuged to obtain 40% ethanol sediment and a first supernatant. And (3) continuously regulating the concentration of ethanol in the first supernatant to 60v%, and centrifuging to obtain 60% ethanol sediment and a second supernatant. Stirring, adjusting ethanol concentration to 80v%, centrifuging to obtain 80% ethanol precipitate and 80% ethanol precipitate supernatant, washing 80% ethanol precipitate with pure ethanol or 95% ethanol for 2-4 times, and volatilizing to remove ethanol to obtain crude rhizoma Polygonati levan.
Further, the above 40v%, 60v% and 80v% ethanol concentration adjustment control can be made to float up and down by 5v%.
In a specific example, the step of extracting the polygama-tion fructan comprises the steps of: weighing 200g of polygonatum cyrtonema, extracting with 10 times of water under reflux for 2 times, each time for 4 hours, filtering, combining the extracting solutions, centrifuging to obtain supernatant, and concentrating to 680mL. 95% ethanol was added to an alcohol concentration of 40%, and the mixture was centrifuged to obtain 40% ethanol sediment and 1370mL supernatant. 783mL of 95% ethanol was added to the above 40% ethanol supernatant with stirring, and the mixture was centrifuged to obtain 60% ethanol sediment and 2000mL of 60% ethanol supernatant. Adding 2667mL of 95% ethanol into the supernatant under stirring, centrifuging to obtain 80% ethanol sediment and 80% ethanol sediment supernatant, washing 80% ethanol sediment with 95% ethanol for 3 times, and volatilizing alcohol to obtain crude rhizoma Polygonati levan.
In a specific example, the method for purifying the component having a weight average molecular weight of 2000Da to 8000Da in the Polygonatum sibiricum polysaccharide is selected from one or more of anion exchange column chromatography, gel exclusion column chromatography, dialysis and ultrafiltration.
Specifically, the crude polygonatum sibiricum levan component is added with deionized water for re-dissolution, a small amount of insoluble matters are removed by centrifugation, and the crude polygonatum sibiricum levan component is purified by methods such as an alcohol precipitation fractionation method, a gel exclusion chromatography method, a dialysis method or an ultrafiltration method, etc., and fractions or trapped liquid or permeate liquid containing levan are collected, and if salt is needed to be desalted, the purified polygonatum sibiricum levan is obtained by directly carrying out vacuum freeze drying or vacuum concentration and then carrying out alcohol precipitation and vacuum drying.
As will be readily appreciated by those skilled in the art, for Gel exclusion chromatography, gel materials such as Sephadex series, polyacrylamide Bio-Gel P series and Gel fillers formed by cross-linking them are reasonably selected according to the molecular weight of polysaccharide substances in the polysaccharide component of Polygonatum sibiricum, and then the column loading, sample loading and sequential elution with saline or non-saline elution solutions are performed according to the actual properties of each filler and the fractions are collected. Because of no characteristic ultraviolet absorption of saccharide, the flow can be detected by sulfuric acid phenol method, etc., the flow curve is drawn, the flow peaks can be combined, the flow can be concentrated or not, the concentrated flow can be put into dialysis bag dialysis or ultrafiltration membrane package ultrafiltration desalination, the desalinated trapped liquid is collected, and the purified polygonatum sibiricum levan is obtained through vacuum freeze drying or vacuum decompression drying. Or purifying rhizoma Polygonati polysaccharide component by dialysis or ultrafiltration, and selecting ultrafiltration membrane with proper molecular weight for tangential flow ultrafiltration interception. For example, the aqueous solution of the polygonatum polysaccharide component is fully dialyzed or ultrafiltered by a dialysis bag or an ultrafiltration membrane bag with the molecular weight larger than that of the polygonatum fructan, permeate or effluent is collected to remove substances with large molecular weight, then the aqueous solution is fully dialyzed or ultrafiltered by a dialysis bag or an ultrafiltration membrane bag with the molecular weight smaller than that of the polygonatum fructan, retentate is collected, concentrated and then vacuum freeze-dried or vacuum reduced-pressure dried to obtain the purified polygonatum fructan.
The invention also provides a structure analysis method of the polygonatum sibiricum levan, which comprises the following steps:
(1) Molecular weight measurement: taking purified Polygonatum sibiricum fructan sample, and optionally analyzing molecular weight and distribution thereof by high performance gel exclusion chromatography-differential detector detection (HPGPC-RI) and matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS).
(2) Monosaccharide composition analysis: taking purified Polygonatum sibiricum fructan sample, hydrolyzing with lower concentration acid to obtain monosaccharide, directly loading into chromatographic column such as sugar-D, and analyzing monosaccharide composition of Polygonatum sibiricum fructan by high performance liquid chromatograph.
(3) Methylation analysis: taking a purified polygonatum levan sample, carrying out partial methylation of polysaccharide hydroxyl groups by using a halomethane reagent such as methyl iodide and the like under alkaline conditions, reducing by using sodium borohydride after hydrolysis under alkaline conditions, carrying out acetylation on methylated sugar alcohol by using anhydride, carrying out extraction and volume fixing, and carrying out gas chromatograph-mass spectrometer (GC-MS) analysis to judge the glycosidic bond connection mode of the polygonatum levan.
(4) And (3) infrared spectrum analysis: taking a purified polygonatum sibiricum levan sample, fully drying, and measuring the infrared spectrum of the sample on an infrared spectrometer by adopting a solid potassium bromide tabletting method.
(5) Nuclear magnetic resonance analysis: dissolving purified rhizoma Polygonati levan sample in deuterated heavy water, lyophilizing, repeating the heavy water exchange for three times, dissolving in heavy water with high deuteration degree, and detecting nuclear magnetic resonance spectrum of sample including one dimension 1 H and 13 c spectrum 1 H- 1 H COSY、 1 H- 1 H TOCSY、 1 H- 1 H ROESY、 1 H- 13 C HSQC、 1 H- 13 cHMBC two-dimensional correlation spectrum.
(6) And (3) comprehensive data analysis: analyzing the analysis data of the steps comprehensively to clarify the chemical structure of the polygonatum sibiricum levan.
According to the structure analysis method, the preparation method is applied to extract and purify the polygonatum sibiricum levan from the polygonatum cyrtonema. The result shows that the polygonatum sibiricum levan only shows one chromatographic peak with good symmetry on the HPGPC gel chromatographic peak, and the weight average molecular weight is 3kDa, and the polydispersity index is 1.20; the monosaccharide composition shows that the Polygonatum sibiricum fructan consists of fructose and glucose; methylation analysis shows that the polygonatum sibiricum levan has beta (2-6) glycosidic bonds and beta (2-1) glycosidic bonds; infrared spectrum analysis shows that the absorption peaks of sugar rings and hydroxyl groups on the rings are consistent with the absorption peak of levan when the absorption signal peaks of furanose are in the fingerprint region; can be clearly attributed to the polygonatum sibiricum levan according to nuclear magnetic resonance spectrum data 1 H and 13 NMR signal of C.
In summary, the polygonatum sibiricum levan has the structural characteristics shown in the general formula I: beta (2-6) glycosidic bond is taken as main chain, and side chain fructose is connected to the main chain by beta (2-1) glycosidic bond.
Figure BDA0004060573920000081
In the formula (I), m, n and p represent the number of sugar repeating units, m, n and p are natural numbers including 0, and m+n > p.
According to the preparation method and the structure analysis method, the polygonatum plant contains polygonatum levan with the structure shown in the chemical formula II.
Figure BDA0004060573920000082
Obviously, the polygonatum fructan with the structure shown in the chemical formula II is a structure when p=0 and m+n > p is required to be satisfied, and the minimum values of m and n are m=n=1.
According to the invention, pharmacological researches have surprisingly found that the polygonatum levan can promote proliferation of probiotics, and has remarkable effect in 2-12 hours; along with the increase of the drug concentration, the capability of the polygonatum levan for promoting probiotics is obviously enhanced, which indicates that the polygonatum levan can promote the proliferation of the probiotics. Therefore, the polygonatum sibiricum levan with the novel structure has the effect of promoting the proliferation of probiotics, and has the practical application value of regulating intestinal flora.
For this reason, the invention further provides the application of the polygonatum sibiricum levan in preparing medicines and/or foods for regulating intestinal flora, promoting digestion and enhancing intestinal peristalsis.
The pharmaceutical composition of an embodiment of the invention comprises the polygonatum levan, pharmaceutically acceptable salts or pharmaceutically acceptable solvents thereof, and pharmaceutically and/or food acceptable auxiliary materials. Optionally, the auxiliary materials comprise pharmaceutically acceptable excipients, carriers and/or diluents and/or flavoring agents and the like.
Further, the pharmaceutical composition also contains pharmaceutical and/or food auxiliary materials. For example containing at least one of an excipient or flavouring agent.
Further, the pharmaceutical composition comprises an effective amount of a polygalactose.
In a specific example, the dosage form of the pharmaceutical composition is injection or solid preparation, such as water injection, freeze-dried powder injection, capsule, effervescent agent and the like, and the auxiliary materials comprise excipient and/or flavoring agent and the like.
The invention also provides application of the pharmaceutical composition in preparation of medicines and/or functional foods for regulating intestinal flora, promoting digestion and enhancing intestinal peristalsis.
An "effective amount" as used herein refers to an amount sufficient to achieve a desired result or to have an effect on an undesired condition. For example, a "therapeutically effective amount" refers to an amount sufficient to achieve a desired therapeutic result or to have an effect on an undesired symptom, but generally insufficient to cause an adverse side effect.
The application of the invention in the directions of regulating intestinal flora, promoting digestion, enhancing intestinal peristalsis and the like refers to the application of the invention which aims at improving and stabilizing pathological conditions or symptoms related to the digestive tract and aims at being given to patients. Including both active treatment, direct amelioration of a pathological condition or disorder; prophylactic treatment, i.e., reducing or partially or completely inhibiting the occurrence of a pathological condition or disorder, is also included. Wherein prevention means to exclude, avoid, eliminate, prevent, refrain from or hinder something from happening, especially by pre-action.
Generally, the above-mentioned medicaments or compositions containing the same of the present invention are used in the preparation of foods or health care products for mammalian (e.g. human) consumption. Of course, it is also contemplated in some cases to apply it to other mammals, such as feeding pet cats, dogs, etc.; or livestock, cattle, horses, pigs, sheep, goats, etc., and others such as mice, rabbits, rats, guinea pigs, etc.
When the Polygonatum sibiricum polysaccharide is prepared into various products, necessary auxiliary materials and/or auxiliary agents can be added. Such as a pharmaceutically acceptable carrier, broadly refers to an aqueous or nonaqueous solution, dispersion, suspension or emulsion, and the like. Non-aqueous solutions may be simply enumerated as including, for example, one or more of ethanol, polyol (e.g., glycerol, propylene glycol, polyethylene glycol, and the like), carboxymethyl cellulose, vegetable oil, and the like. If used to prepare oral dosage forms, such as tablets, capsules, other fillers, disintegrants, sustained release agents, preservatives, dispersants, and the like may also be included. By way of example, the preservative may be parabens, chlorobutanol, sorbic acid, and the like.
The present invention will be described in detail below with reference to specific embodiments in conjunction with the attached drawings, but these embodiments do not limit the scope of the claims of the present invention in any way.
The starting materials, compounds, compositions or components used in the examples below were obtained primarily commercially or prepared using techniques generally known to those skilled in the art. Next, the experimental operation method of the preparation process method in the following examples is carried out according to general experimental guidance, and experimental parts in the examples are exemplified in forms clear to those skilled in the art, not limited to the methods in the following examples. The person skilled in the art can carry out the necessary derivations according to conventional knowledge to obtain an experimental procedure suitable for the particular starting material.
EXAMPLE 1 extraction and purification of Polygonatum sibiricum fructan
1. Polygonatum sibiricum polysaccharide extraction
Weighing 200g of polygonatum cyrtonema, extracting with 10 times of water under reflux for 2 times, each time for 4 hours, filtering, combining the extracting solutions, centrifuging to obtain supernatant, and concentrating to obtain 680mL of concentrated solution. 495mL of 95v% ethanol was added to an alcohol concentration of 40v%, and the mixture was centrifuged (4000 rpm. Times.15 min) to obtain 40% ethanol sediment and supernatant. 783mL of 95v% ethanol was added to the 40v% ethanol supernatant under stirring until the ethanol concentration reached 60v%, and the mixture was centrifuged (4000 rpm. Times.15 min) to obtain 60v% ethanol sediment and supernatant. Adding 2667mL 95v% ethanol into 60% ethanol supernatant under stirring until the ethanol concentration reaches 80v%, centrifuging (4000 rpm×15 min) to obtain 80v% ethanol precipitate and supernatant, washing 80% ethanol precipitate with 95% ethanol for 3 times, and volatilizing to obtain crude rhizoma Polygonati levan. Wherein, the alcohol can be volatilized by reduced pressure evaporation to remove ethanol.
2. Polygonatum sibiricum levan purification
Dissolving the crude rhizoma Polygonati levan in deionized water, loading on DEAE-52 (3.2 cm×30 cm) weak anion exchange column, eluting with deionized water and 0.1, 0.5 and 1.0M NaCl solution, respectively, and collecting eluate about 10mL per tube. Detecting by a phenol sulfuric acid method, drawing an outflow curve, collecting the last fraction which contains no or a small amount of polysaccharide, concentrating under reduced pressure, and freeze-drying under vacuum to obtain 22.83g of purified polygonatum levan.
Specifically, after elution, 10mL of elution fraction is collected per tube, the elution fraction is analyzed by a high performance liquid chromatograph, fractions containing levan are collected, reduced pressure concentration and freeze vacuum drying are carried out, and the purified polygonatum levan is obtained.
EXAMPLE 2 structural resolution of Polygonatum sibiricum levan
1. Experimental procedure
1.1. Molecular weight and distribution thereof
The molecular weight and distribution of the polyghace seche prepared in example 1 were analyzed by high performance gel exclusion chromatography-differential detector detection (HPGPC-RI).
Chromatographic instrument: agilent technologies 1260series high performance liquid chromatograph;
chromatographic conditions: shodex Ohpak SB-804HQ (7.8 mm. Times.300 mm) column; the column temperature is 35 ℃; a differential detector; the mobile phase is NaCl with the concentration of 0.1M and the flow rate is 0.5mL/min;
The measuring process comprises the following steps: 5mg of Polygonatum sibiricum levan sample or dextran reference substance with known molecular weight is taken and added with mobile phase to prepare 5mg/mL solution, the solution is filtered by a microporous membrane with 0.22 mu m, 50 mu L of filtered solution is analyzed by a high performance liquid chromatograph, and a chromatogram is recorded. The data are processed by GPC software, a standard curve is drawn, the data are brought into an equation, and the molecular weight is calculated.
Analysis of molecular weight and distribution of Polygonatum sibiricum fructan by matrix assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS)
Instrument: bruker UltrafleXtreme matrix assisted laser desorption ionization time-of-flight mass spectrometer;
instrument conditions: linear positive ion mode; the N2 laser source and the matrix are 2, 5-dihydroxybenzoic acid.
The measuring process comprises the following steps: weighing a certain amount of polygonatum levan sample, adding deionized water to prepare a solution, mixing 1 mu L of sample solution with 1 mu L of matrix solution, then spotting on a sample target, naturally drying, measuring the molecular weight of the polygonatum levan, and recording a mass spectrogram. The data were processed using flexAnalysis software to calculate molecular weight.
1.2 analysis of monosaccharide composition
About 20mg of a Polygonatum sibiricum levan sample is taken and placed in 2 COD test tubes, 2mL of 0.05M sulfuric acid solution is added into the test tubes for dissolution, hydrolysis reaction is carried out for 40min in an oven at 80 ℃, after the reaction is finished, cooling is carried out to room temperature, the mixture is taken out and placed in a 5mL EP tube (centrifuge tube), barium carbonate solid powder is added for neutralizing sulfuric acid, and the mixture is centrifuged (15000 rpm is multiplied by 15 min) to obtain a supernatant, and the supernatant is subjected to chromatography through a 0.22 mu m microporous filter membrane.
Chromatographic conditions: instrument: agilent technologies 1260series high performance liquid chromatograph; chromatographic column: cosmosil Sugar-D column (2.0 mm. Times.250 mm,5 μm); the column temperature is 30 ℃; the mobile phase is acetonitrile and water (85:15); flow rate: 0.2mL/min; the sample injection volume is 20 mu L; and a RID detector.
1.3 methylation analysis
Methylation reaction: weighing 10mg of Polygonatum sibiricum levan sample, placing in a 5mL reaction bottle, dissolving in 4mL DMSO, sequentially adding 100mg of sodium hydroxide and 600 mu L of methyl iodide, and performing ultrasonic reaction at room temperature under nitrogen filling for 1h. After the reaction, 2mL of pure water was added, the pH of the solution was adjusted to neutrality with 1M hydrochloric acid solution, 6mL of chloroform was added for extraction, and the organic phase was dried under reduced pressure at 40℃and then vacuum dried for 12 hours.
Acid hydrolysis of methylated polysaccharides: the dried sample was dissolved in 2mL of 0.5M TFA solution and subjected to acid hydrolysis at 120℃for 5 hours in a sealed tube.
Reduction of methylated monosaccharides: the pH of the hydrolysate is adjusted to 10-12 by using 1M NaOH, 50mg of sodium borohydride is added, the mixture is stirred for 2 hours in a water bath at 50 ℃, 250 mu L of glacial acetic acid is added to terminate the reaction, and the reaction solution is freeze-dried.
Acetylation of methylated sugar alcohols: 1mL of pyridine and 1mL of acetic anhydride are added into the freeze-dried sample, and the mixture is subjected to tube sealing reaction at 100 ℃ for 1h in a test tube. After cooling, 1mL of water was added and 2mL of dichloromethane were extracted 3 times each time, and the organic phase was extracted with N 2 After blow-drying, 100. Mu.L of methylene chloride was added for dissolution and GC-MS analysis was performed.
GC conditions: HP-5MS quartz capillary column (50 mm. Times.0.25 mm,0.25 μm); column temperature: the initial temperature is 80 ℃, the temperature is kept for 11min, the temperature is programmed to be 5 ℃/min to 250 ℃ and the temperature is kept for 5min; the carrier gas is high-purity helium; the flow rate of the carrier gas is 1.5mL/min; the temperature of the sample inlet is 270 ℃; the pre-column pressure is 100KPa; not split; sample injection amount: 2. Mu.L.
MS conditions: ionization mode EI; electron energy 70; the temperature of the transmission line is 290 ℃; the ion source temperature is 230 ℃; the temperature of the quadrupole rods is 150 ℃; the mass range is 50-600; and (5) adopting a CCRC standard spectrum library computer to search and qualify.
1.4 Infrared Spectrometry analysis
According to the fourth part of 2020 edition of Chinese pharmacopoeia, a solid tabletting method is adopted: taking 2mg of sample, vacuum drying at 40 ℃ for 24 hours, tabletting by KBr, and carrying out 4000-400 cm on the sample by using a Tensor 27 Fourier transform mid-infrared spectrometer -1 Scanning and recording a spectrogram.
1.5 Nuclear magnetic resonance analysis
Dissolving 10mg of Polygonatum sibiricum levan sample in 0.5mL D 2 O, freeze-drying, D 2 After three O exchanges, the lyophilized sample was dissolved in 0.5mL D 2 O (99.9 atom% D, containing 0.05wt.%3- (trimethyllyl) propionic-2, 3-d as internal standard) 4 acid, sodium salt). Determination using Bruker 800MHz Nuclear magnetic resonance spectrometer 1 H/ 13 C NMR spectrum and two-dimensional spectrum [ ] 1 H- 1 H COSY、 1 H- 1 H TOCSY、 1 H- 1 H ROESY、 1 H- 13 CHMBC、 1 H- 13 C HSQC). And analyzing and processing the detection result by adopting MestReNova software.
2. Experimental results
The characteristics of the polygonatum sibiricum levan of the invention are as follows: the white solid is odorless, is easily dissolved in water, is slightly dissolved in organic solvents such as ethanol, and has hygroscopicity.
The results of high performance gel exclusion chromatography (HPGPC-RI) showed (FIG. 1) that the Polygonatum sibiricum fructan had only one symmetrical chromatographic peak with a weight average molecular weight (Mw) of 3803Da and a polydispersity of 1.21. The result of matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS) shows (figure 2), the molecular weight range of the polygonatum fructan is 2000.9-7027.5Da, and the corresponding molecular weight of the polygonatum fructan with the highest intensity is 2973.7Da.
The analysis result of monosaccharide composition shows (figure 3), the HPLC chart of the Polygonatum sibiricum fructan after being directly subjected to acid hydrolysis into a Sugar-D column shows that only one peak of fructose and glucose exists, wherein the peak area of the fructose is far higher than that of the glucose, which indicates that the monosaccharide composition of the Polygonatum sibiricum fructan only contains fructose and glucose, the glucose content is far lower than that of the fructose, and the structure characteristics of the fructan are met.
Methylation analysis results show that the polygonatum fructosan has beta (2-6) fructosyl glycosidic bond, beta (2-1, 6) fructosyl glycosidic bond, beta (-2) fructosyl glycosidic bond, beta (2-1) fructosyl glycosidic bond and alpha (1-6) glucosyl glycosidic bond.
As shown in FIG. 4, the infrared spectrum (cm -1 ) The data are: 3385cm -1 The characteristic absorption of the stretching vibration of the hydroxyl O-H on the sugar ring; 2935cm -1 Stretching and vibrating the methylene C-H on the sugar ring; 1129-1028 cm -1 C-O-C stretching vibration on the sugar ring; 933cm -1 And 878cm -1 Is the configurational characteristic absorption of beta-D-fructose.
The analysis results of nuclear magnetic resonance NMR detection are shown in FIG. 5 and FIG. 6, and are detailed 1 H and 13 the assignment of the C NMR signals is shown in tables 1 and 2. Chemical shift 5.43ppm is 1, 6-O-alpha-D-glucose (1, 6-O-alpha-D-Glcp, abbreviated as G) and the proton signal at the 6-position (H-6 and H-6') of 1, 6-O-alpha-D-Glcp is 4.08 (3.77) ppm and its corresponding chemical shift of C-1 is 95.0ppm. The chemical shift described above is significantly shifted to the lower field relative to the corresponding chemical shift of unsubstituted glucose, indicating that the hydroxyl groups at positions 1 and 6 of the glucosyl group G are substituted. The coupling constant of the anomeric C-H of the glucosyl group G is 180Hz, which shows that the anomeric configuration is alpha type. Fructose is furanose, which has no anomeric hydrogen at the 2-position and has only an anomeric carbon signal in the NMR spectrum, and the anomeric carbon signal has 4 anomeric carbon signals in the region, and the chemical shift values are 106.9, 106.7, 106.5 and 106.1ppm from high to low, respectively corresponding to 2, 6-O-beta-D-fructosyl (2, 6-O-beta-D-Fruf, abbreviated as A), 1,2, 6-O-beta-D-fructosyl (1, 2, 6-O-beta-D-Fruf, abbreviated as B), 2-O-beta-D-fructosyl (2-O-beta) C-2 signal of-D-Fruf, abbreviated as D) and 2, 1-O-beta-D-fructosyl (2, 1-O-beta-D-Fruf, abbreviated as E)
1 H and 13 c remote correlation HMBC spectra clearly show that H-6 (G6) of glucosyl G and C-2 (D2) of D have obvious correlation signals; h-1 (B1) of fructosyl B and C-2 (E2) of fructosyl E have related signals, H-6 (B6) of fructosyl B and C-2 (A2) of fructosyl A have related signals, H-6 (A6) of fructosyl A and C-2 (B2) of fructosyl B have strong related signals, H-1 (E1) of fructosyl E and C-2 (E2) of fructosyl E. Therefore, the levan is connected in the following manner: fructosyl A forms the main chain with a β (2.fwdarw.6) glycosidic bond, and fructosyl E is linked to the main chain of fructosyl A twice with a β (2.fwdarw.1) glycosidic bond.
From the above data, the chemical structural formula of the novel polygonatum sibiricum levan is shown in the general formula I.
Figure BDA0004060573920000131
In the formula (I), m, n and p represent the number of sugar repeating units, m, n and p are natural numbers including 0, and m+n > p.
The structure is characterized in that: (1) The Polygonatum sibiricum levan is a purified polysaccharide extracted from rhizome of Polygonatum sibiricum and is plant levan; (2) In the monosaccharide composition of the polygonatum sibiricum levan, fructose is the main component and a small amount of glucose; the monosaccharide connection mode of the polygonatum sibiricum levan is as follows: beta (2-6) glycosidic bond is taken as main chain, and side chain fructose is connected to the main chain through beta (2-1) glycosidic bond. The arrangement mode of the polygonatum sibiricum levan is not disclosed and reported by the domestic and foreign public literature search, and is found for the first time by the inventor.
TABLE 1 Polygonatum sibiricum levan 1 Assignment of H NMR
Figure BDA0004060573920000141
TABLE 2 Polygonatum sibiricum levan 13 Assignment of C NMR
Figure BDA0004060573920000142
Wherein, the liquid crystal display device comprises a liquid crystal display device,
2, 6-O-beta-D-Fruf represents → 6) -beta-D-Fruf- (2 →;
1,2, 6-O-beta-D-Fruf represents → 1, 6) -beta-D-Fruf- (2 →;
2-O-beta-D-Fruf represents beta-D-Fruf- (2-;
2, 1-O-beta-D-Fruf represents → 1) -beta-D-Fruf- (2 → 2;
1,6- α -D-Glcp means → 1) - α -D-Glcp- (6 →.
Example 3 experiment of promoting the growth of probiotics with Polygonatum sibiricum levan
1. Test article, reagent and strain
Polygonatum sibiricum levan, abbreviated as DP1, was prepared as in example 1; sugar-free Man-Rogosa-Sharpe (MRS) medium, beijing coolaber company; the 4 strains were respectively bifidobacterium animalis subspecies (Bifidobacterium animalis subsp. Lactis), bifidobacterium bifidum (Bifidobacterium bifidum), lactobacillus acidophilus (Lactobacillus acidophilus) and lactobacillus plantarum (Lactobacillus plantarum) all purchased from the beijing north narrative biotechnology institute.
2. Experimental method
2.1 preparation of solutions
600mg of Fructooligosaccharide (FOS), 600mg of Polygonatum sibiricum levan (DP 1) and 700mg of Glucose (Glucose) were weighed separately, 10mg/mL solutions were prepared with sterile water, and after filtration through a 0.22 μm sterile filter, each solution was added to a sugarless MRS medium at a concentration of 5 mg/mL. 56mL of sterilized MRS medium was taken, 56mL of sterile water was added to dilute the filtered sample, and after mixing, 5mg/mL MRS medium was prepared. B.bifidum and L.plantarum were inoculated on the corresponding media 1X 10 7 CFU/mL amount, B.analytis subsp.lactis and L.acidophilus were inoculated 1X 10 on the corresponding medium 8 Amount of CFU/mL.
2.2 bacterial culture
Bacterial culture medium: MRS (L-cysteine) medium contains 0.05% L-cysteine;
bacterial recovery: 1 relative liquid culture medium and 2 plates were prepared, a small grinding wheel was used to make a circle on the 1/2 position of the tube body in an ultra clean bench, then 75vol% alcohol was used to wipe the tube wall, and the tube wall was broken with forceps. Injecting 0.3-0.5mL of liquid culture medium into a freeze-drying tube, gently blowing and mixing, fully dissolving into bacterial suspension, sucking 1/3 of the bacterial suspension, adding the rest of the bacterial suspension into 2 plates on average, and culturing under corresponding conditions until bacterial colonies grow out.
And (3) expanding bacteria: single colonies were picked from the plates and grown on a shaker at 37℃in liquid medium.
Seed preservation: taking 25mL of bacterial liquid, centrifuging at 4 ℃ (4100 rpm multiplied by 10 min), removing supernatant, adding 2.5mL of liquid culture medium to suspend and precipitate, adding equal volume of glycerol, mixing, and freeze-preserving at-80 ℃.
2.3 cultivation of the respective bacterial Medium
Each bacterial strain was placed in an anaerobic environment (85% N) 2 ,10％CO 2 And 5%H 2 ) All in volume percent, were incubated, and samples were taken at each time point of 0h, 2h, 4h, 6h, 8h, 12h, 24h, 36h, and 48 h.
2.4 detection of pH
The culture broth was removed at each sampling time point, three replicates were taken, and after centrifugation (4000 rpm. Times.10 min), the pH was measured 3 times per time point.
2.5 detection of OD value
The culture solution was taken out at each sampling time point, three portions were taken in parallel, and after shaking up and down for 15s, poured into a cuvette, and the OD value was measured with an ultraviolet spectrophotometer at a wavelength of 600nm, 3 times for each time point.
2.6 detection of bacterial count
The culture solution was taken out at each sampling time point, three portions were taken in parallel, and after shaking up and down for 15 seconds, a certain amount of the culture solution was taken out therefrom, diluted by a certain multiple, and counted on a blood cell counting plate, 3 times for each time point.
3. Experimental results
3.1 Effect on pH in Medium for various bacteria
As shown in FIG. 7, the pH of the Polygonatum sibiricum fructan and other treated groups was reduced to a different extent than the control group, indicating that the bacterial strains increased in the presence of Polygonatum sibiricum fructan (DP 1), fructo-oligosaccharides (FOS) and Glucose (Glucose) as a result of short chain fatty acid metabolites such as lactic acid and acetic acid, thereby lowering the pH of the medium.
3.2 Effect on OD values in respective bacterial Medium
As shown in FIG. 8, the OD value of the culture solutions obtained by culturing with different culture mediums is rapidly increased from 0 to 12 hours, and the OD value of each culture solution is basically unchanged after 12 hours; the OD values of the Polygonatum sibiricum fructan (DP 1), fructooligosaccharide (FOS) and Glucose (Glucose) groups were all significantly higher than the blank group for the corresponding time period. Polygonatum sibiricum fructan, fructo-oligosaccharide and glucose can promote proliferation of various bacterial strains, and the proliferation quantity of bacteria after Polygonatum sibiricum fructan treatment is obviously different from that of a blank group.
3.3 Effect on the number of bacterial plants in each bacterial Medium
As shown in FIG. 9, in the culture medium containing Polygonatum sibiricum levan, 4 bacterial strains all enter an exponential phase in 2-12 hours and proliferate rapidly; comparing the maximum bacterial strain number of 24h or 48h bacteria entering the platform phase, the Polygonatum sibiricum levan group is equivalent to the oligomeric levan group and the glucose group, and is obviously higher than that of the blank group; the proliferation conditions of 4 different strains are different according to the strains, and the proliferation promoting activity of the bifidobacterium animalis subsp.
The results prove that the polygonatum sibiricum levan has remarkable effect of promoting the growth activity of probiotics, so that the polygonatum sibiricum levan with the novel structure has practical application values of improving intestinal flora, promoting digestion and enhancing intestinal peristalsis.
EXAMPLE 4 preparation of Polygonatum sibiricum levan lyophilized powder injection
1. Material
The method of example 1 is repeated to obtain Polygonatum sibiricum fructan and pharmaceutical grade sodium chloride.
2. Prescription of prescription
Names of raw and auxiliary materials Dosage of
Polygonatum sibiricum levan 50g
Sodium chloride 4.5g
Water for injection 500mL
Is co-manufactured into 1000 pieces
3. Preparation process
Weighing the prescription amount of the polygonatum sibiricum levan and the sodium chloride, adding the injection water to the full amount, stirring to dissolve completely, and sterilizing by an intermittent hot-pressing method. Adding 0.3% of medicinal active carbon, and stirring for 20min; the heat source was removed by decarbonation filtration using a buchner funnel and a 3.0 μm microporous filter membrane. Filtering with microporous membrane of 0.22 μm after the content is qualified; subpackaging in controlled penicillin bottles, each bottle is 0.5mL, half-pressing and plugging, putting into a freeze drying box, freeze-drying according to a set freeze-drying curve, pressing and plugging, discharging, capping, qualified visual inspection and packaging to obtain a finished product.
And (3) freeze-drying: feeding the sample into a box, cooling the partition plate to-40 ℃, and keeping the temperature for 4 hours; the cold trap was cooled to-50℃and a vacuum was started to 250 μbar. Sublimation is started: heating to-20deg.C for 1 hr at uniform speed, and maintaining for 3 hr; heating to-10 ℃ at constant speed for 3 hours, maintaining for 8 hours, and maintaining 100-250 mu bar in vacuum; and drying: heating to-5 ℃ for 2 hours, maintaining for 2 hours, and maintaining 150-200 mu bar in vacuum; heating to 10 ℃ for 0.5h, maintaining for 2h, and maintaining 80-100 mu bar in vacuum; raising the temperature to 40 ℃ for 0.5h, keeping the temperature for 4h, and vacuumizing to the minimum.
Example 5 preparation of Polygonatum sibiricum levan Capsule
1. Material
A Polygonatum sibiricum fructan, food or pharmaceutical grade starch obtained by the method of example 1.
2. Prescription of prescription
Names of raw and auxiliary materials Dosage of
Polygonatum sibiricum levan 300g
Starch 500g
Is co-manufactured into 5000 granules
3. Preparation process
Weighing the prescription amount of the polygonatum sibiricum levan and the starch, and stirring to completely mix. Adding appropriate amount of pulvis Talci, granulating with ethanol, sieving, drying, and filling into No. 2 capsule shell, wherein each capsule body is filled with 60mg of rhizoma Polygonati levan to obtain rhizoma Polygonati levan capsule.
Example 6 preparation of Polygonatum sibiricum levan oral liquid
1. Material
The Polygonatum sibiricum levan obtained by the method of example 1 and the food or pharmaceutical grade flavoring agent are aspartame and apple essence.
2. Prescription of prescription
Figure BDA0004060573920000171
Figure BDA0004060573920000181
3. Preparation process
Weighing rhizoma Polygonati levan, aspartame and apple essence, adding purified water, completely dissolving, filtering with microporous membrane of 0.22 μm, packaging with oral liquid tank according to 2mL per bottle, sealing, and sterilizing.
Example 7 preparation of Polygonatum sibiricum levan facial cleanser
1. Material
The Polygonatum sibiricum fructan obtained in the method of example 1 is prepared from raw materials in cosmetic grade or food grade.
2. Prescription of prescription
7g of polygonatum levan, 2.0g of glycerin, 4.0g of butanediol, 0.5g of propylene glycol, 0.1g of EDTA sodium, 0.3g of guar gum, 2.0g of zinc dioxide, 4.0g of C12-15 alcohol benzoate, 3.0g of C12-20 alkyl glucoside, 0.5g of C14-22 alcohol, 1.2g of cetostearyl alcohol, 0.2g of nipagin ester, 0.3g of sodium stearate, 0.5g of polydimethylsiloxane alcohol, 0.2g of polysorbate and 55g of deionized water.
3. Preparation process
Weighing a prescription amount of polygonatum sibiricum levan, and adding purified water for complete dissolution; completely dissolving glycerol, butanediol, propylene glycol, EDTA sodium, guar gum, zinc dioxide, sodium stearate, C12-15 alcohol benzoate, C12-20 alkyl glucoside, C14-22 alcohol, cetostearyl alcohol, and purified water; taking the nipagin ester, the polydimethylsiloxane alcohol and the polysorbate, and adding purified water for complete dissolution. Mixing the above prepared solutions, stirring thoroughly, filtering with microporous membrane of 0.22 μm, canning the filtrate with 30mL per bottle, sealing, and sterilizing.
Example 8 preparation of Polygonatum sibiricum levan mask
1. Material
The Polygonatum sibiricum fructan obtained in the method of example 1 is prepared from raw materials in cosmetic grade or food grade.
2. Prescription of prescription
12g of polygonatum sibiricum levan, 3.5g of transparent xanthan gum, 2.0g of hydrolyzed hyaluronic acid, 2.0g of sodium hyaluronate, 0.3g of propylene glycol, 0.5g of carbomer, 0.1 g of nipagin ester, 0.25g of PPG-10 methyl glucose ether, 1.0g of glyceroglycoside and 45g of deionized water.
3. Mask preparation process
And 1, adding deionized water, transparent xanthan gum, hydrolyzed hyaluronic acid, sodium hyaluronate, propylene glycol, carbomer and nipagin ester into an emulsifying pot, heating to 75 ℃ while stirring, preserving heat after the temperature is reached, continuing stirring until the solution is transparent and uniform, preserving heat for 20 minutes, and cooling to 42 ℃.
Step 2, ph=7.6±0.2.
And 3, continuously cooling to 35 ℃, adding PPG-10 methyl glucose ether, polygonatum sibiricum levan and glyceroglycosides, continuously stirring uniformly, and discharging after the inspection is qualified. The nonwoven substrate was coated at a coating weight of 2.1g per square centimeter.
The descriptions of the patents, patent applications, and publications cited in this application are incorporated by reference in their entirety. Any references cited should not be construed as allowing them to be used as "prior art" to the present application.
The technical features of the above-described embodiments may be arbitrarily combined, and all possible combinations of the technical features in the above-described embodiments are not described for brevity of description, however, as long as there is no contradiction between the combinations of the technical features, they should be considered as the scope of the description.
The above examples illustrate only a few embodiments of the invention, which are described in detail and are not to be construed as limiting the scope of the invention. It should be noted that it will be apparent to those skilled in the art that several variations and modifications can be made without departing from the spirit of the invention, which are all within the scope of the invention. Accordingly, the scope of protection of the present invention is to be determined by the appended claims.

Claims (6)

1. The polygonatum sibiricum levan is characterized by being prepared by the following steps:
s1, extracting rhizome crude polysaccharide of Polygonatum plants:
weighing 200g of polygonatum cyrtonema, extracting with 10 times of water under reflux for 2 times, each time for 4 hours, filtering, combining the extracting solutions, centrifuging to obtain supernatant, and concentrating to 680mL;
adding 95% ethanol until the ethanol concentration is 40%, centrifuging to obtain 40% ethanol sediment and 1370mL supernatant;
Adding 783mL of 95% ethanol into the 40% ethanol precipitation supernatant under stirring, and centrifuging to obtain 60% ethanol precipitate and 2000mL of 60% ethanol precipitation supernatant;
adding 2667 mL of 95% ethanol into the supernatant under stirring, centrifuging to obtain 80% ethanol sediment and 80% ethanol sediment supernatant, washing 80% ethanol sediment 3 times with 95% ethanol, and volatilizing alcohol to obtain crude rhizoma Polygonati levan;
s2, purifying the polygonatum sibiricum levan:
dissolving the crude rhizoma Polygonati levan in deionized water, loading on DEAE-52 weak anion exchange column, eluting with deionized water and 0.1, 0.5 and 1.0M NaCl solution respectively, and collecting eluate about 10mL per tube; analyzing with high performance liquid chromatograph, collecting fractions containing levan, concentrating under reduced pressure, and freeze vacuum drying to obtain purified rhizoma Polygonati levan;
the Polygonatum sibiricum levan has a structure represented by the following general formula I
Figure QLYQS_1
In the formula (I), m, n and p represent the number of sugar repeating units, m, n and p are natural numbers, and m+n>p；
The weight average molecular weight of the polygonatum fructan is 2000 Da to 8000 Da, and the polydispersity coefficient is 1 to 3.
2. A pharmaceutical composition comprising an effective amount of the levan of claim 1.
3. The pharmaceutical composition according to claim 2, wherein the pharmaceutical composition is in the form of a lyophilized powder, an oral liquid or a capsule.
4. Use of a polyghace-ing fructan according to claim 1, for the preparation of a food and/or a medicament for regulating the gastrointestinal flora, promoting intestinal peristalsis and/or promoting digestion.
5. The use of the polygalactose according to claim 4, comprising polygalactose and pharmaceutically acceptable salts or pharmaceutically acceptable solvents thereof, and pharmaceutically and/or food acceptable excipients.
6. The use of the polyghace seche fructan according to claim 5, characterized in that the auxiliary materials comprise pharmaceutically acceptable excipients, carriers and/or diluents and/or flavouring agents.
CN202310056112.8A 2021-03-09 2021-03-09 Polygonatum sibiricum levan, pharmaceutical composition and application Pending CN116253806A (en)

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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101555290A (en) * 2009-05-07 2009-10-14 上海交通大学 Method for preparing radix astragali homopolysaccharide
CN107827995A (en) * 2017-12-18 2018-03-23 大理大学 A kind of method for extraction and purification of P. kingianum polysaccharide
CN112062872A (en) * 2020-10-15 2020-12-11 扬州市博睿糖生物技术有限公司 Polygonatum sibiricum homogeneous polysaccharide and preparation method and application thereof

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101456918B (en) * 2009-01-16 2011-05-11 韩金光 Sealwort polysaccharide and preparation method thereof, and its application in preparing hypoglycemic and reducing blood lipid drugs
CN101851297A (en) * 2010-05-06 2010-10-06 安徽天品堂生态农业有限公司 High-efficiency extraction process of polygahatous plysaccharides
EP2420243A1 (en) * 2010-08-18 2012-02-22 Inovativo Biomedicinas Tehnologiju Instituts, SIA Compositions obtainable from bred beetroot juice to promote iron absorption and blood forming
CN102491999A (en) * 2011-11-15 2012-06-13 重庆市秀山红星中药材开发有限公司 Method for extracting polygonatum rhizome oligosaccharide
CN102351960B (en) * 2011-11-15 2013-07-10 重庆市秀山红星中药材开发有限公司 Method for extracting rhizoma polygonati polysaccharide
CN105153319B (en) * 2014-06-05 2018-09-04 上海家化联合股份有限公司 A kind of application of sealwort paragalactan and preparation method thereof and anti-inflammatory aspect
CN104530253B (en) * 2015-02-03 2019-02-26 上海中医药大学附属龙华医院 A kind of column chromatography separation method and extracting method of Siberian solomonseal rhizome polysaccharide
CN111087481A (en) * 2018-10-23 2020-05-01 南通安广美术图案设计有限公司 Extraction method of polygonatum polysaccharide
CN109354630A (en) * 2018-12-13 2019-02-19 常馨尹 The extracting method of Siberian solomonseal rhizome polysaccharide
CN112076205B (en) * 2020-09-16 2022-03-15 中国人民解放军空军军医大学 Application of polygonatum polysaccharide in preparation of medicine for treating ulcerative colitis

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101555290A (en) * 2009-05-07 2009-10-14 上海交通大学 Method for preparing radix astragali homopolysaccharide
CN107827995A (en) * 2017-12-18 2018-03-23 大理大学 A kind of method for extraction and purification of P. kingianum polysaccharide
CN112062872A (en) * 2020-10-15 2020-12-11 扬州市博睿糖生物技术有限公司 Polygonatum sibiricum homogeneous polysaccharide and preparation method and application thereof

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