CN116251034A - Composition with free radical removal effect and application thereof - Google Patents

Abstract

The invention relates to the technical field of cosmetics, and discloses a composition with a free radical removal effect and application thereof. The composition with the effect of scavenging free radicals provided by the invention comprises the following components in parts by mass: 3-o-ethyl ascorbic acid: 1-3 parts; ascorbyl tetraisopalmitate: 2-4 parts; tocopherol: 0.3 to 0.7 part; acetylcysteine: 0.1 to 0.3 part. The composition is prepared by mixing 3-o-ethyl ascorbic acid, ascorbyl tetraisopalmitate, tocopherol and acetylcysteine according to a certain weight portion, has a synergistic effect in the aspect of scavenging free radicals, and can obtain better skin antioxidation and anti-aging effects with lower application amount.

Description

Composition with free radical removal effect and application thereof

Technical Field

The invention relates to the technical field of cosmetics, in particular to a composition with a free radical removal effect and application thereof.

Background

In the metabolic process of the organism, after one electron of oxygen is taken out, an oxygen atom without paired electrons becomes a free radical. The free radical is unstable, has strong oxidizing property and strong electron absorbing capability, and needs to abstract electrons again in cell molecules of body tissues to pair the cell molecules, and when the cell molecules push out new electrons, the cell molecules become free radicals and also need to rob electrons in cell membranes or cell nucleus molecules, so that new free radicals are continuously generated. The free radical can cause protein denaturation and crosslinking, so that a plurality of enzymes and hormones in the body lose biological activity, the immunity, the nerve reflex, the exercise capacity and other system activities of the body are reduced, and simultaneously, the nucleic acid structure can be destroyed, the whole body is caused to be abnormal in metabolism and the like, and finally, the body is diseased.

The oxygen inhaled into human body from air is converted into superoxide anion by enzyme, which is used to kill virus and other exotic invaders, and the excessive superoxide anion becomes free radical for promoting cell aging. The human body can generate endogenous antioxidant substances to catalyze and decompose superoxide anion free radicals, so that the human body reaches a safe balance state. However, with the increase of age, the endogenous antioxidant level of human body is gradually reduced, and at this time, the excessive superoxide anion free radical attacks the unsaturated phospholipids on the cell membrane, so that the cells age prematurely, pigmentation is generated, senile plaque is formed, and the human body is aged.

Disclosure of Invention

The invention aims to provide a composition with the effect of scavenging free radicals and application thereof.

To solve the above-mentioned problems, a first aspect of the present invention provides a composition having an effect of scavenging free radicals, comprising, in parts by mass: 3-o-ethyl ascorbic acid: 1-3 parts; ascorbyl tetraisopalmitate: 2-4 parts; tocopherol: 0.3 to 0.7 part; acetylcysteine: 0.1 to 0.3 part.

Preferably, the composition with the effect of scavenging free radicals provided by the invention comprises the following components in parts by mass: 3-o-ethyl ascorbic acid: 2 parts; ascorbyl tetraisopalmitate: 3 parts; tocopherol: 0.5 parts; acetylcysteine: 0.2 parts.

A second aspect of the present invention provides the use of a composition having free radical scavenging efficacy according to the first aspect of the present invention in cosmetics and/or skin care products.

In the application provided by the invention, the cosmetic and/or skin care product is applied to the outer surface of the skin.

Preferably, in the application provided by the invention, the cosmetic and/or skin care product is selected from one or more of eye cream, face cream, essence, emulsion and facial mask essence.

Preferably, in the application provided by the invention, the composition with the effect of scavenging free radicals is dispersed as an additive in a matrix raw material, wherein the matrix raw material is selected from one or more of oily raw materials, powdery raw materials and colloid raw materials.

Preferably, in the application provided by the present invention, the cosmetic and/or skin care product further comprises: surfactant, essence and perfume, pigment, humectant, antiseptic, antioxidant, ultraviolet absorbent, chelating agent, astringent, permeation promoter, and nutritional additive.

Compared with the prior art, the invention adopts 1 to 3 parts of 3-o-ethyl ascorbate and tetraisopalmitate ascorbate: 2-4 parts of tocopherol: 0.3 to 0.7 part of acetylcysteine: 0.1 to 0.3 part, and realizes the synergistic effect. Therefore, the composition with the effect of scavenging free radicals provided by the invention can play an obvious role in resisting oxidation and aging of skin under the condition of low application amount when applied to cosmetics and/or skin care products.

Drawings

FIG. 1 is a graph comparing DPPH radical scavenging activity of groups Et-VC, VC-IP, VE, NAC, et-VC/VC-IP/VE/NAC;

FIG. 2 is a graph of DPPH radical scavenging activity vs. that of Et-VC group, VC-IP group, VE group, NAC group, and comparative group 1;

FIG. 3 is a graph of DPPH radical scavenging activity vs. that of Et-VC group, VC-IP group, VE group, NAC group, and comparative group 2.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, embodiments of the present invention will be described in detail below. However, those of ordinary skill in the art will understand that in various embodiments of the present invention, numerous technical details have been set forth in order to provide a better understanding of the present application. However, the technical solutions claimed in the present application can be implemented without these technical details and with various changes and modifications based on the following embodiments.

The embodiment of the invention provides a composition with the effect of scavenging free radicals, which comprises the following components in parts by mass: 3-o-ethyl ascorbic acid: 1-3 parts; ascorbyl tetraisopalmitate: 2-4 parts; tocopherol: 0.3 to 0.7 part; acetylcysteine: 0.1 to 0.3 part.

In some embodiments of the present invention, provided compositions having free radical scavenging efficacy comprise, in parts by mass: 3-o-ethyl ascorbic acid: 2 parts; ascorbyl tetraisopalmitate: 3 parts; tocopherol: 0.5 parts; acetylcysteine: 0.2 parts.

The composition with the effect of scavenging free radicals provided by the embodiment of the invention can be applied to cosmetics and/or skin care products, and the cosmetics and/or skin care products are applied to the outer surface of skin.

In some embodiments of the present invention, the cosmetic and/or skin care product to which the composition having the effect of scavenging free radicals is applied is selected from one or more of eye cream, face cream, essence, lotion, mask essence.

In some embodiments of the present invention, the composition having the effect of scavenging free radicals is dispersed as an additive in a matrix material selected from one or more of an oleaginous material, a powdered material, and a colloidal material.

Wherein, the oily raw material can be any one or a combination of the following matrix raw materials: soybean oil, olive oil, almond oil, castor oil, peanut oil, cotton seed oil, tea seed oil, jojoba oil, avocado oil, coconut oil, palm oil, cocoa butter, rice bran oil, evening primrose oil, wheat germ oil, corn germ oil, palm wax, wood wax, mink oil, turtle oil, snake oil, egg yolk oil, tallow, horse fat, lard, deer fat, lecithin, lanolin, beeswax, whale wax, shellac wax, paraffin, vaseline, microcrystalline wax, ozokerite, squalane, lanolin wax, lanolin alcohol esters, acetylated lanolin alcohols, lanolin acids, lanolin acid esters, polyoxyethylene lanolin alcohol ethers, polyoxypropylene lanolin alcohol ethers, hydrogenated lanolin, alkoxylated hydrogenated lanolin, silicone oils (e.g., dimethicone oil, octamethyl silicone oil, methyl phenyl polysiloxane), polysiloxane-polyoxyalkyl block copolymers, methyl hydrogen silicone oils, cn fatty acids and esters thereof, cn fatty alcohols and esters thereof; wherein n.gtoreq.10, and preferably 10 to 24, more preferably 12 to 20, such as 14, 16, 17, 18.

Such Cn fatty acids as: lauric acid, myristic acid, palmitic acid, stearic acid, oleic acid, linoleic acid, erucic acid, and capric acid.

Such as Cn fatty alcohols: lauryl alcohol, cetyl alcohol, stearyl alcohol, isostearyl alcohol, oleyl alcohol, cocoyl alcohol.

The Cn fatty acid esters, cn fatty alcohol esters are as follows: isopropyl myristate, myristyl lactate, cetyl lactate, isopropyl palmitate, isooctyl palmitate, butyl stearate, isooctyl stearate, monoglyceride stearate, polyethylene glycol stearate, decyl oleate, glyceryl caprylate, glyceryl caprate, and glyceryl trioleate.

The powdery raw material can be any one or a combination of the following matrix raw materials: mica, magnesium carbonate, titanium dioxide, zinc white powder, calcium hydroxide, calcium carbonate, kaolin, calcium stearate, zinc stearate, magnesium stearate, talcum powder, silicon dioxide, aluminum hydroxide, calcium pyrophosphate, calcium bicarbonate and bentonite.

Wherein the oil raw material can be any one or more of plant oil raw material, animal oil raw material, mineral oil raw material, synthetic oil raw material and semisynthetic oil raw material. Such as: starch, cyclodextrin, xanthan gum, carrageenan, guar gum, gum arabic, gum tragacanth, agar, shellac, sodium alginate, gelatin, methylcellulose, ethylcellulose, sodium carboxymethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose, a quaternary ammonium salt of a poly cellulose ether, polyvinyl alcohol, polyvinylpyrrolidone, sodium polyacrylate, polyoxyethylene, vinylpyrrolidone/vinyl acetate copolymers, carbopol resins, magnesium aluminum silicate, C12-15 alcohol benzoates.

Wherein the solvent raw material can be any one or a combination of the following matrix raw materials: water, ethanol, propanol, isopropanol, isobutanol, ethylene glycol, 1, 2-propanediol, cyclohexane, dichlorodifluoromethane, tetrafluorodichloroethane, ethylene glycol monoethyl ether, diethylene glycol monoethyl ether, acetone, methyl isobutyl ketone, ethyl acetate, butyl acetate, amyl acetate, dibutyl phthalate.

In some embodiments of the present invention, the cosmetic and/or skin care product to which the composition having the effect of scavenging free radicals is applied further comprises: surfactant, essence and perfume, pigment, humectant, antiseptic, antioxidant, ultraviolet absorbent, chelating agent, astringent, permeation promoter, and nutritional additive.

Wherein the surfactant may be: 1) Anionic surfactants such as: c12-14 fatty alcohol ammonium sulfate, C12-14 fatty alcohol polyoxyethylene ether carboxylate, dodecyl sulfate diethanol amine salt, C12-14 fatty alcohol polyoxyethylene ether ammonium sulfate, dodecyl sulfate triethanolamine, alcohol ether phthalate monoester sodium salt, acylated peptide, lauric acid monoethanolamide sodium sulfate, N-octanol polyoxypropylene ether succinate monoester sulfonate, cetyl alcohol succinate monoester sulfonate, sulfosuccinic acid monoester disodium salt, succinic acid monostearyl disodium salt, alcohol ether sulfosuccinic acid monoester disodium salt, fatty alcohol polyoxyethylene ether (sodium) sulfosuccinic acid monoester ammonium salt, oleamide sulfosuccinic acid monoester disodium salt, N-acyl glutamic acid potassium salt, pyrrolidone sodium carboxylate, sodium polyacrylate, fatty alcohol polyoxyethylene ether phosphate monoester and its salt, fatty alcohol polyoxyethylene ether phosphate monoester ethanol amine salt, fatty alcohol polyoxyethylene ether phosphate monoester ethanolamine salt, alkylphenol polyoxyethylene ether phosphate monoester and its salt, nonylphenol ether phosphate monoester ethanol salt, alkyl phosphate monoester salt, phthalic acid monolauryl ester salt, zinc undecylenate, anionic amino acid surfactant, N-C12-sodium acyl glutamate, N-acyl sodium L-acyl glutamic acid monoester sodium salt, sodium stearyl glutamate monoester sodium salt, and fatty alcohol polyoxyethylene ether phosphate monoester ethanol amine salt; 2) Nonionic surfactants such as: oleyl alcohol polyoxyethylene ether (such as emulsifier VO series), alkylphenol polyoxyethylene ether (such as emulsifier OPE series), polyoxyethylene stearate, glycol monostearate, glycol distearate, polyethylene glycol monostearate, polyethylene glycol distearate, polyethylene glycol dilaurate, glycerol distearate, glycerol tristearate, sucrose stearate, polyoxyethylene glyceryl ether monostearate, N-lauroyl glutamate dialkanol ester, fatty alcohol benzoate, sorbitol monofatty acid ester (Span, span such as Span-20, span-60, span 65, span-80, span-83, span-85), polyoxyethylene ether sorbitol monofatty acid ester (Tween, tween such as Tween-20, tween-40, tween-60, tween-61, tween-80), diethanolamine, triethanolamine, fatty acid monoethanolamide (such as coconut oleic acid monoethanolamide, lauric acid monoethanolamide, palmitic acid monoethanolamide), coconut oleic acid diethanolamide, lauric acid diethanolamide, methyl glucoside stearate, polyoxyethylene methyl glucoside stearate, glycol glucoside stearate, propylene glycol glucoside stearate, C8-alkyl glucoside, lauryl glucoside, polyoxyethylene glucoside, glyceryl monostearate, mink, glyceryl ester of mink, glyceryl stearate; 3) Cationic surfactants such as C12-18 alkyl trialkylammonium chloride (e.g., cetyl dimethyl alkyl ammonium chloride, stearyl trimethyl ammonium chloride, cetyl trimethyl ammonium chloride), oleamide propyl dihydroxypropyl dimethyl ammonium chloride, dihydroxypropyl dimethyldodecyl ammonium chloride, dimethyldiallyl ammonium chloride/acrylamide copolymer, oleamide propyl-2, 3-dihydroxypropyl dimethyl ammonium chloride, oleamide propyl dimethyl ethyl ammonium ethylsulfate, bis-C12-18 yl dimethyl ammonium chloride, polyquaternary ammonium-11 conditioner, M-505 polyquaternary ammonium-7 conditioner, mink oleamide propyl amine-chitosan, mink oleamide propyl amine-hydrolyzed protein, cationic protein peptides, guar gum-hydroxypropyl trimethyl ammonium chloride, poly cellulose ether quaternary ammonium salt, triethanolamine monooleate, DNP series; 4) Zwitterionic surfactants such as C12-18 alkyl dihydroxyethyl betaine, N-C12-18 alkyl-N- (2-hydroxyethyl) -N- (2-formamidoethyl) ammonium acetate, C12-18 alkoxy hydroxypropyl betaine, cocoamidopropyl amine oxide, sodium cocoisethionate, octadecyl dimethyl amine oxide, cocodiethanol amine oxide, N-alkyl-beta-aminopropionyl diethanol amine, lauroyl propylamine oxide, N-C12-18 acyl glutamic acid, hydroxyethyl decanoic imidazoline betaine, hydroxyethyl myristic imidazoline betaine, hydroxyethyl palmitic imidazoline betaine.

Wherein the flavour and fragrance may be selected from: 1) The natural perfume is used for the food, such as Artemisia absinthium L, eucalyptus globulus oil, anise oil, white lemon oil, white butyl essence, oleum Caryophylli, flos Magnoliae oil, flos Magnoliae essence, folium Isatidis oil, cedarwood oil, peppermint oil, rhizoma Atractylodis stearin, sweet clover essence, orange leaf oil, flos Caryophylli Ocimi oil, flos Jasmini sambac essence, wintergreen oil, radix Angelicae sinensis essence, lichen acid, lignum Santali albi oil, bitter orange leaf oil, fructus Caricae essence, sweetgum essence, cinnamomum camphora oil, herba Artemisiae Argyi, oleum Verniciae Fordii, oleum Olivarum, oleum Eucalypti, oleum Olivarum, oleum Foeniculi, and oleum Foeniculi sassafras oil, cedar oil, sweet osmanthus extract, tangerine oil, carrot seed oil, castoreum, yellow sunflower oil, yellow orchid oil, patchouli oil, artemisia oil, murraya extract, acacia extract, ginger oil, orange oil, peppermint oil, chrysanthemum extract, cumin oil, ambergris, spearmint oil, civet, wintergreen oil, mugwort extract, costustoot oil, michelia Miqueliana oil, rose bay oil, rose oil, jasmine extract, oyster oil the preparation method comprises the following steps of (1) black red extract, black red oil, eucalyptus oil, lemon oil, qilixiang extract, leaf oil, celery seed oil, nutmeg oil, cinnamon oil, litsea cubeba oil, mink balm tincture, narcissus extract, pine needle oil, tree moss extract, jasmine extract, musk balm, sweet orange oil, sandalwood oil, tuberose extract, tagetes essential oil, fennel oil, sabina chinensis oil, citronella oil, geranium oil, carnation oil, perilla oil, sclarea oil, nutgrass galingale oil, bergamot peppermint oil, vetch oil, elsholtzia herb extract, raspberry rose oil, raspberry rose extract, lavender oil, bay leaf oil, ylang oil, coriander seed oil, vanilla oil, bergamot rose extract, bay oil, orange peel oil, iris oil, tobacco flower oil, citronella leaf oil, gardenia extract, amorpha oil, violet leaf extract, guaiacol, 4-methyl guaiacol, 4-ethyl guaiacol, natural vanillin, camphor; 2) The essence is synthesized and the essence is prepared, such as limonene, longifolene, ethyl caryophyllene, isolongifolene ketone, bromostyrol, diphenylmethane, diphenyl ether, m-methyl diphenyl ether, eugenol, isoeugenol, ethyl naphthalene methyl ether, ethyl naphthalene ethyl ether, p-methyl anisole, isoeugenol benzyl ether, rose ether, sandal ether, narcissus (or jasmine ether), ambrox, methyl cypress ether, epoxycedrene, elindol N, 3-hexenol decyl alcohol, lauryl alcohol, benzyl alcohol, phenethyl alcohol, p-methoxybenzyl alcohol, cinnamyl alcohol, dimethylbenzyl alcohol, geraniol, nerol, linalool, rosyl alcohol, terpineol, citronellol, racemic menthol, borneol, cedarwood alcohol, cedarwood ketone, methyl cedarwood ketone, 3, 4-dioxymethylene benzyl alcohol, alpha-methyl-3, 4-dihydro methylene phenylpropionaldehyde, 2-tert-butyl-4-methylcyclohexanol, sclareol, santalol, 3-methyl-5- (2 ',2',3' -trimethylcyclopenten-1 ' -yl) pentan-2-ol, benzaldehyde, acetophenone, phenylacetic acid, p-methylacetophenone, laural, cinnamaldehyde, cinnamic acid, alpha-amyl-beta-phenylacrylal, alpha-phenylpropionaldehyde, mugwort aldehyde, neomugwort aldehyde, lagomorph's ear aldehyde, 3, 4-dioxymethylene benzaldehyde, 3, 7-dimethyl-6-octenal, 3, 7-dimethyl-7-hydroxy octanal, citral, b-cyclohomocitral, elcosyl aldehyde, citrus green aldehyde, 4-hydroxy-3-methoxybenzaldehyde, alpha-dihydrodamascone, beta-damascone, ionone, 6-methyl ionone, nerone, carvone, piperonyl acetone, raspberry ketone, herbal ketone, cis-jasmone, heptanal glycol acetal, phenylacetaldehyde acetal, octanal acetal, alpha-amyl cinnamaldehyde acetal, citral diethyl acetal, anisaldehyde acetal, mushroom aldehyde, phyllocene, hyacinth, apple ester, jeep ketal, geranyl formate, citronellyl formate, avermectin acetate, leaf alcohol acetate, benzyl acetate, phenethyl acetate, cinnamyl acetate, terpineyl acetate, borneol acetate, linalyl acetate, geranyl acetate, p-t-butylcyclohexyl acetate, o-t-butylcyclohexyl acetate, vetiveryl acetate, amber acetate, and avermectin acetate, decalin acetate, cedryl acetate, geranyl propionate, trichloromethyl benzyl acetate, benzyl butyrate, leaf alcohol butyrate, geranyl butyrate, phenethyl 2-methylpentanoate, p-cresol isobutyrate, methyl 2-nonenoate, ethyl benzoate, methyl benzoate, leaf alcohol benzoate, benzyl benzoate, ethyl phenylacetate, p-cresol phenylacetate, ethyl cinnamate, butyl salicylate, isobutyl salicylate, isoamyl salicylate, leaf alcohol salicylate, hexyl salicylate, benzyl salicylate, phenethyl salicylate, cyclohexyl salicylate, jasmonate, methyl 2-pentylcyclopentanone acetate, nerin, gamma-nonolactone, gamma-undecalactone, coumarin, indole, 3-methylindole, 3, 7-dimethyl-2, 6-octadienenitrile, P-methoxybenzonitrile, xylene musk, musk ketone, 2, 6-dinitro-3-methoxy-4-tertiary butyl toluene, 1-dimethyl-4-acetyl-6-tertiary butyl indane, hexamethyl tricyclic isochroman musk, 1,1,2,3,3,6-hexamethyl-5-acetyl indane, musk-T, musk-M, musk-L, musk-F, 11-oxahexadecanolide, and tolna musk; 3) Essence, rose essence, jasmine essence, sandalwood essence and brandy essence.

Wherein the pigment can be one or more selected from the following components: zirconium dioxide, lead acetate, silver nitrate, chlorophyll, copper chlorophyll, ferric ferrocyanide, guanine, fast red, food red I, vat red I, carmine, food red, food cherry red, food red 17, amaranth, fast green, food yellow 3, food lemon yellow, food sudan yellow, food indigo, food blue 2, fluorescent peach red, beta-carotene, henna, gardenia red pigment, gardenia green pigment, gardenia blue pigment, gardenia yellow pigment, titanium mica pearlescent pigment, iron oxide red, iron oxide yellow, iron oxide black, chromium oxide green, bismuth oxychloride, basic peach red, vermilion R, quinoline yellow, shikonin, lac red pigment, ultramarine, acid red 87, acid green 25, acid fluorescent yellow, acid orange 7, golden red 53, lithol Barba red BK, lithol Barbar 2R, permanent orange.

Wherein, the preservative can be one or a combination of several components selected from the following components: sorbic acid and salts thereof, propionate salts, parabens, benzyl alcohol, benzoic acid and salts thereof, 5-chloro-2-methyl-4-isothiazolin-3-one, pinus koraiensis, imidazolidinyl urea, dehydroacetic acid and salts thereof, sodium sulfite, sodium bisulfite, sodium metabisulfite, 2-bromo-2-nitro-1, 3-propanediol (brobol), erythorbic acid and salts thereof, kojic acid and esters thereof, azelaic acid, usnic acid, resorcinol.

Wherein, the antioxidant can be one or a combination of several components selected from the following components: SOD, butyl hydroxy anisole, gallate, ascorbic acid and its salt, isoascorbic acid and its salt, sitosterol, rutin, and tocopherol.

Wherein the humectant can be one or a combination of several components selected from the following components: 1, 2-butanediol, sorbitol, xylitol, glycerol, lactate, polyethylene glycol, DL-pyrrolidone carboxylate, D-glucose, kojic acid and its esters, uric acid, orotic acid, pectic acid, laminin, collagen, trehalose, 12, and cholesterol hydroxystearate.

Wherein the ultraviolet absorber can be one or a combination of several components selected from the following components: pyridoxine hydrochloride, kojic acid and its esters, rutin, aloin, caffeic acid, hesperetin, 2-ethylhexyl salicylate, 2-ethylhexyl p-dimethylaminobenzoate, 2-ethylhexyl p-methoxycinnamate, phenyl salicylate, lipo aminobenzoate, 2-hydroxy-4-methoxybenzophenone-5-sulfonic acid, 2-hydroxy-4-octoxybenzophenone.

Wherein the chelating agent can be one or a combination of several components selected from the following components: EDTA disodium salt, zinc bipyridyl thioketone and sodium thiosulfate.

Wherein the astringent can be one or a combination of several components selected from the following components: phenol is used for preparing zinc sulfonate, aluminum hydroxychloride, aluminum sulfate, aluminum potassium sulfate, zinc sulfate, aluminum chloride, zinc chloride and karamine.

Wherein, the permeation assisting agent can be one or a combination of several components selected from the following components: dimethyl sulfoxide, 1-n-dodecyl azepan-2-one, 2-aminoethanol.

Wherein, the pH value regulator can be one or a combination of several components selected from the following components: tartaric acid, lactic acid, boric acid, succinic acid.

Wherein, the nutritional additive can be one or a combination of several components selected from the following components: chitin, chitosan, silk peptide, silk fibroin, lecithin, epidermal growth factor, trehalose, lactose, pearl powder, collagen hydrolysate, hyaluronic acid and salts thereof, egg membrane extract, phytosterol, chlorlysozyme, chondroitin sodium sulfate, lac Regis Apis, propolis, mel, glycyrrhizic acid, glycyrrhetinic acid, gamma-linolenic acid, kojic acid and esters thereof, gibberellic acid, glycine, L-aspartic acid and salts thereof, L-cystine and salts thereof, DL-alanine, L-serine, DL-serine, L-methionine, DL-methionine, L-lysine hydrochloride, L-threonine, inositol, maltitol, pantothenate, D-panthenol, retinol acetate, allantoin, pyridoxine hydrochloride, cortisone, nicotinamide, vitamins, estrogens, hematoxylin, uric acid, orotic acid, aloin, azelaic acid, abietic acid, usogenic acid, alizarin, limonene, baicalein, deoxygenistein, orange peel, seleno, diglucoside, phenylglyoxylic acid, tributyl, tri-methyl-propyl-ammonium chloride, and cholic acid.

Experimental example

The instruments, kits, cells and reagents used in the experimental examples are all commercially available through public commercial sources, and are instruments, kits, cells and reagents commonly used in the art, and the operation steps of the instruments and kits are performed according to the instruction of the instructions.

1. Purpose of experiment

The scavenging effect of the experimental and control groups on free radicals was evaluated using DPPH free radical scavenging assay.

2. Main experimental raw materials and reagents

Raw material 1:

name: 3-o-ethyl ascorbic acid; code number: et-VC; traits: white to off-white crystalline powder.

Raw material 2:

name: ascorbyl tetraisopalmitate; code number: VC-IP; purity: traits: colorless to pale yellow transparent oily liquid.

Raw material 3:

name: tocopherols; code number: VE; traits: yellow to brownish red viscous oily liquid.

Raw material 4:

name: acetylcysteine; code number: NAC; traits: white crystalline powder.

Reagent 1:

DPPH:100 mg/bottle, purchased from: sigma.

3. Experimental method

(1) Preparing DPPH working solution: a10 mM DPPH solution (500 ul) was taken and diluted to 25ml (0.2 mM) with absolute ethanol.

(2) DMSO or ddH for test or control substance ₂ O was prepared as a mother liquor and diluted to a suitable concentration with serum-free medium or PBS to obtain the test substances of the groups shown in table 1, respectively:

TABLE 1

(3) The DPPH reagent was used to test the radical scavenging ability of the test substance in each of the test group and the control group, and each of the test group and the control group was provided with the following test system A, B, C, D (20 ul of test substance, 180ul of DPPH):

a: the volume of the substance to be detected is replaced by PBS (phosphate buffer solution) without the substance to be detected and only DPPH;

b: the volume of the substance to be detected and DPPH are not added, the volume of the substance to be detected is replaced by PBS, and the volume of the DPPH is replaced by absolute ethyl alcohol;

c: adding the substance to be detected and DPPH;

d: and no DPPH is added, only the substance to be detected is added, and the volume of DPPH is replaced by absolute ethyl alcohol.

The detection steps are carried out in a 96-well plate, at least 3 multiple wells are arranged, required objects to be detected, DPPH, PBS or absolute ethyl alcohol are added, then a row gun is used for mixing uniformly, and the mixture is placed for 30min at room temperature in a dark place.

(4) Absorbance values (0.01 mg/ml EGCG was used as a positive control in this experiment) were measured at 540nm for detection system A, B, C, D to obtain absorbance a, absorbance B, absorbance C, and absorbance D, respectively, and DPPH removal activity (%) was calculated for each experimental group and comparative group.

DPPH scavenging Activity calculation:

(5) Data entry and statistical analysis:

DPPH scavenging activity was statistically processed using Excel entries and Prism (Version 7) packages and expressed as mean.+ -. Standard error. One-way analysis of variance was performed between groups, and p <0.05 was considered statistically different.

4. Experimental results

FIG. 1 is a graph comparing DPPH radical scavenging activity of groups Et-VC, VC-IP, VE, NAC, et-VC/VC-IP/VE/NAC;

FIG. 2 is a graph of DPPH radical scavenging activity vs. that of Et-VC group, VC-IP group, VE group, NAC group, and comparative group 1;

FIG. 3 is a graph of DPPH radical scavenging activity vs. that of Et-VC group, VC-IP group, VE group, NAC group, and comparative group 2.

In fig. 1: n=3, mean±sem. "indicates p <0.05 compared to the Et-VC/VC-IP/VE/NAC group and" indicates p < 0.001 compared to the Et-VC/VC-IP/VE/NAC group.

Fig. 2 and 3: n=3, mean±sem. "" means p <0.05 compared to the control group, "" means p < 0.001 compared to the control group.

According to fig. 1, the Et-VC/VC-IP/VE/NAC group (wherein, in parts by mass: et-VC: VC-IP: VE: nac=2:3:0.5:0.2) was administered, the Et-VC/VC-IP/VE/NAC group had a significantly increased radical scavenging activity compared to the Et-VC group, VC-IP group, VE group, NAC group administered alone, indicating that the Et-VC/VC-IP/VE/NAC group had a synergistic effect in terms of radical scavenging activity.

According to fig. 2 and 3, either control group 1 (wherein: et-VC: VC-IP: VE: nac=2:3:0.5:0.01) or control group 2 (wherein: et-VC: VC-IP: VE: nac=2:3:0.5:0.02) was administered, the control group 1 and control group 2 did not have a synergistic effect on free radical scavenging activity compared to the Et-VC group, VC-IP group, VE group, NAC group administered alone.

It will be understood by those of ordinary skill in the art that the foregoing embodiments are specific examples of carrying out the invention and that various changes in form and details may be made therein without departing from the spirit and scope of the invention.

Claims (7)

1. A composition with the effect of scavenging free radicals, which is characterized by comprising the following components in parts by mass:

3-o-ethyl ascorbic acid: 1-3 parts;

ascorbyl tetraisopalmitate: 2-4 parts;

tocopherol: 0.3 to 0.7 part;

acetylcysteine: 0.1 to 0.3 part.

2. The composition having the effect of scavenging free radicals according to claim 1, characterized by comprising, in parts by mass:

3-o-ethyl ascorbic acid: 2 parts;

ascorbyl tetraisopalmitate: 3 parts;

tocopherol: 0.5 parts;

acetylcysteine: 0.2 parts.

3. Use of the composition having the effect of scavenging free radicals as claimed in any one of claims 1 to 2 in cosmetics and/or skin care products.

4. The use according to claim 3, characterized in that the cosmetic and/or dermatological product is applied to the external surface of the skin.

5. The use according to claim 3, wherein the cosmetic and/or skin care product is selected from one or more of eye cream, face cream, essence, lotion, mask essence.

6. The use according to claim 3, wherein the composition having the effect of scavenging free radicals is dispersed as an additive in a matrix material selected from one or more of an oleaginous material, a powdered material, a colloidal material.

7. The use according to claim 3, wherein the cosmetic and/or skin care product further comprises: surfactant, essence and perfume, pigment, humectant, antiseptic, antioxidant, ultraviolet absorbent, chelating agent, astringent, permeation promoter, and nutritional additive.

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