CN1162450A - Method for collecting and processing fresh deer blood - Google Patents
Method for collecting and processing fresh deer blood Download PDFInfo
- Publication number
- CN1162450A CN1162450A CN96104430A CN96104430A CN1162450A CN 1162450 A CN1162450 A CN 1162450A CN 96104430 A CN96104430 A CN 96104430A CN 96104430 A CN96104430 A CN 96104430A CN 1162450 A CN1162450 A CN 1162450A
- Authority
- CN
- China
- Prior art keywords
- blood
- sanguis cervi
- sanguis
- deer
- sterilization
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 210000004369 blood Anatomy 0.000 title claims abstract description 53
- 239000008280 blood Substances 0.000 title claims abstract description 53
- 241000282994 Cervidae Species 0.000 title claims abstract description 35
- 238000000034 method Methods 0.000 title claims abstract description 23
- 238000012545 processing Methods 0.000 title abstract description 6
- 230000001954 sterilising effect Effects 0.000 claims abstract description 15
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 15
- 238000010241 blood sampling Methods 0.000 claims description 12
- 239000000203 mixture Substances 0.000 claims description 10
- 238000002360 preparation method Methods 0.000 claims description 10
- 241000411851 herbal medicine Species 0.000 claims description 9
- 238000003672 processing method Methods 0.000 claims description 8
- 239000003146 anticoagulant agent Substances 0.000 claims description 7
- 229940127219 anticoagulant drug Drugs 0.000 claims description 7
- 239000010836 blood and blood product Substances 0.000 claims description 7
- 229940125691 blood product Drugs 0.000 claims description 7
- 150000001875 compounds Chemical class 0.000 claims description 6
- 235000016709 nutrition Nutrition 0.000 claims description 6
- 238000012856 packing Methods 0.000 claims description 6
- 239000003814 drug Substances 0.000 claims description 5
- 238000000926 separation method Methods 0.000 claims description 5
- 239000004615 ingredient Substances 0.000 claims description 4
- 239000007788 liquid Substances 0.000 claims description 4
- 239000000843 powder Substances 0.000 claims description 4
- 210000002966 serum Anatomy 0.000 claims description 4
- 206010002091 Anaesthesia Diseases 0.000 claims description 3
- 241000208340 Araliaceae Species 0.000 claims description 3
- 241000222336 Ganoderma Species 0.000 claims description 3
- 239000009636 Huang Qi Substances 0.000 claims description 3
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 claims description 3
- 235000003140 Panax quinquefolius Nutrition 0.000 claims description 3
- 230000037005 anaesthesia Effects 0.000 claims description 3
- 210000000601 blood cell Anatomy 0.000 claims description 3
- 239000002131 composite material Substances 0.000 claims description 3
- 239000000706 filtrate Substances 0.000 claims description 3
- 235000008434 ginseng Nutrition 0.000 claims description 3
- 210000002381 plasma Anatomy 0.000 claims description 3
- 238000000746 purification Methods 0.000 claims description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 2
- 239000002738 chelating agent Substances 0.000 claims description 2
- 239000012467 final product Substances 0.000 claims description 2
- 239000008103 glucose Substances 0.000 claims description 2
- 238000002791 soaking Methods 0.000 claims description 2
- 239000006228 supernatant Substances 0.000 claims description 2
- 241000283007 Cervus nippon Species 0.000 abstract description 3
- 201000010099 disease Diseases 0.000 abstract description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 2
- 241000282985 Cervus Species 0.000 abstract 1
- 230000002429 anti-coagulating effect Effects 0.000 abstract 1
- 238000004806 packaging method and process Methods 0.000 abstract 1
- 241001465754 Metazoa Species 0.000 description 6
- 102000053602 DNA Human genes 0.000 description 4
- 108020004414 DNA Proteins 0.000 description 4
- 102000019197 Superoxide Dismutase Human genes 0.000 description 4
- 108010012715 Superoxide dismutase Proteins 0.000 description 4
- 230000032683 aging Effects 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000003307 slaughter Methods 0.000 description 4
- 238000009395 breeding Methods 0.000 description 3
- 230000001488 breeding effect Effects 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 241000283026 Cervus elaphus Species 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 230000004071 biological effect Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 230000001256 tonic effect Effects 0.000 description 2
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- ATJFFYVFTNAWJD-UHFFFAOYSA-N Tin Chemical compound [Sn] ATJFFYVFTNAWJD-UHFFFAOYSA-N 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 230000001195 anabolic effect Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 229940126678 chinese medicines Drugs 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 210000001840 diploid cell Anatomy 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 239000004459 forage Substances 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 210000004731 jugular vein Anatomy 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 239000000155 melt Substances 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000002516 radical scavenger Substances 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 208000011580 syndromic disease Diseases 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
Landscapes
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
A method for collecting and processing the fresh blood of sika deer and red deer includes such steps as collecting fresh blood from living deers in a septic condition, anticoagulating, purifying, proportioning with other components, sterilization and packaging. The obtained product has bioactivity for a certain period and can delay sanility and prevent more senile diseases.
Description
The present invention relates to a kind of to animal in deer family, the particularly collection and the processing method of Cervus nippon Temminck and Cervus elaphus linnaeus intravital blood.
Chinese medicine thinks that Sanguis cervi has tangible medical care effect, and modern medicine has also confirmed this point by test.The Norman Bethune Medical University has carried out experimental study to the aging resistance effect of In vitro culture diploid cell clearly with regard to Sanguis cervi, and contrast by experiment confirms that Sanguis cervi has the DNA of promotion anabolic effect clearly.Another experiment is to investigate Sanguis cervi clearly to the influence of rat blood serum LPO and SOD, and by contrast, clear rat feeding LPO obviously reduces with Sanguis cervi, and SOD significantly improves.Above-mentioned description of test fresh deer blood can promote body aging cell DNA (deoxyribonucleic acid) (DNA) synthetic, improves superoxide dismutase (SOD) activity, prevents to pile up with scavenger cylinder super-oxidation lipid (LPO), thereby plays the aging resistance effect.
Record in China's " middle pharmacopeia ": Sanguis cervi, after slaughtering with its blood directly bubble go in the wine or collect in the container, make thinner, airing or oven dry, be cut into small pieces, collect standby.In the ancient book also useful iron pipe insert directly that deer gets in vivo that blood is drunk or its blood directly contained when slaughtering deer container and drink record.These all are that traditional Sanguis cervi is collected and processing method.For many years, people process various Sanguis cervi goods, fundamentally, all still are to carry out on the basis of traditional method.
Cervus nippon Temminck is the first-grade state protection wild animal, and Cervus elaphus linnaeus is national wildlife under second class protection.To these precious wildlife resources, at first to protect, guarantee that it can normal existence and procreation, just can rationally utilize then.And said method be by to deer slaughter or the health of heavy damage deer is collected Sanguis cervi, these all are to violate national relevant laws regulation.
In addition, collect Sanguis cervi according to the method described above, one is unhygienic; Two old blood or the blood dry powder that come deer to slaughter back preparation have destroyed most bioactive substances in the Sanguis cervi liquid, and very easily by pathogen contamination or rot; Moreover the Sanguis cervi of getting alive can only be confined to there and then a few peoples and take, and can not realize suitability for industrialized production, is difficult to apply.
The objective of the invention is provides a kind of under the prerequisite of not destroying animal health at above problem, scientifically stable breeding deer group live body is regularly carried out the collection and the method for processing of fresh blood, and can keep the biological activity of blood products over a period to come, but and suitability for industrialized production.
The objective of the invention is to realize by following measure: the collection of fresh deer blood and processing method are included in one or several process procedures such as blood collection under the aseptic condition, anticoagulant, separation purification, other active ingredients of compatibility, sterilization, packing.
The acquisition method of fresh deer blood: select the healthy deer that lives, earlier through health check-up and sterilization, again through anesthesia or fixing, the sterilization blood taking bag that connects 400～800ml then with No. 16 blood taking needles of improved lengthening, under aseptic condition, blood taking needle is thrust the deer vena systemica, slowly adopt to prescribed dose with active method or passive means; Take out blood taking needle, preserve or make freeze dried powder under-20 ℃～4 ℃ the temperature and preserve being placed into after the bright Sanguis cervi packing of gathering, the sterilization.Anticoagulant and nutritional solution are arranged in the above-mentioned blood taking bag.
The processing method of fresh deer blood comprises separation purification, compatibility Sanguis Anseris domestica or the Chinese medicinal ingredients of fresh blood, is specially:
1, the separation of fresh deer blood is purified
The preparation of composition blood: the bright Sanguis cervi that will gather is centrifugal under 4000～5000rpm condition with centrifuge under aseptic condition, 15 minutes, collects blood plasma and cell composition.
Serum preparation: the bright Sanguis cervi that will gather solidified under aseptic condition 12～24 hours, and is centrifugal under 4000～5000rpm condition then, 15 minutes, collects supernatant and gets final product.
2) compatibility Sanguis Anseris domestica or Chinese medicinal ingredients in the fresh deer blood promptly prepare compound blood products:
A, Sanguis cervi and Sanguis Anseris domestica are processed into compound blood products by 1: 0.5～1 (weight proportion), regulate pH value 7.0～7.5.
The soaking in Chinese liquor liquid of b, Sanguis cervi and Chinese herbal medicine is mixed with composite article, and the proportioning of Chinese herbal medicine is (consumption is a weight portion):
Radix Ginseng 1～3 Radix Astragali 5～20 Ganodermas 2～8 Fructus Lyciis 5～20
Said herbal medicine is soaked in the 60 degree Chinese liquor, and the weight proportion of Chinese herbal medicine and Chinese liquor is 1: 5, and soak time is 20 days, will soak filtrate and bright Sanguis cervi mix homogeneously, packing, sterilization then, and the weight proportion of bright Sanguis cervi and Chinese medicine soak solution is 4: 1.
According to design of the present invention, bright Sanguis cervi and said herbal medicine can be formed various prescriptions, are prepared into to have slow down aging, give the health care tonic of anti-multiple Senile disease and deficient syndrome.
The present invention is a kind of stable breeding deer group's advanced person, the living animal blood sampling and blood processing method of science of being used for.This method is under the prerequisite of not damaging deer body health, and regularly quantitatively blood sampling makes traditional first resource become long-term resource, has protected precious animal, has reduced the blood sampling cost; Whole process is all carried out under aseptic condition, can keep the biological activity of blood and goods over a period to come, guarantees health, safety, effective; This method can make collection, the processing of live body Sanguis cervi realize suitability for industrialized production, for the collection processing of Sanguis cervi and other living animal blood with opened up new approach.
Further describe collection of the present invention and processing method below in conjunction with embodiment.
One, the preparation of blood sampling deer: select health deer to count head, method for breeding is raised routinely, takes a blood sample the last week, can add an amount of trace element in the feedstuff, as zinc, magnesium, stannum etc.The fasting in preceding 8～12 hours of taking a blood sample.Need with the protein rich forage feed a few days after the blood sampling, blood sampling cycle is 7～15 days, a blood sampling volume≤800ml.
Two, the preparation before the blood sampling:
The blood sampling deer is carried out physical examination, sterilization, anesthesia or fixing.
Three, blood sampling and blood storage:
Connect the sterilization blood taking bag of 400m1 with No. 16 long blood taking needles of 40mm, slowly blood is extracted out at deer jugular vein or limbs vein place or utilized the negative pressure sucking-off, anticoagulant and nutritional solution are arranged in the blood bag, anticoagulant adopts Ca
++Chelating agent, nutritional solution are glucose.Anticoagulant and nutritional solution have 46ml in the blood taking bag of 400ml, adopt the conventional method preparation.
The bright Sanguis cervi of gathering can be preserved 10～15 days in 0 ℃～4 ℃ environment through sterilization; In-10 ℃～-20 ℃ environment, can preserve 15 days～3 months (time spent melts) in 5～10 ℃ of water-baths; Also can adopt lyophilizing to preserve,, make freeze dried powder, can preserve 1～2 year, melt with normal saline or distilled water during use with freeze dryer freezing draining under vacuum state.
Four, the processing of Sanguis cervi:
Prepare composition blood according to preceding method, collect blood plasma and cell composition; Preparation serum.
The preparation of Sanguis cervi composite article:
(1) Sanguis cervi and Sanguis Anseris domestica are made compound blood products by 1: 0.5 (weight proportion) mix homogeneously, and regulating pH value is 7.4, in case precipitation.
(2) Radix Ginseng, the Radix Astragali, Ganoderma, Fructus Lycii were pressed 1: 5: 2: 5 weight proportion, be immersed in 5 times in 60 ° of Chinese liquor of Chinese medicine weight, will soak filtrate and bright Sanguis cervi after 20 days and make the nourishing liquid that has defying age, fills blood, can building body by 1: 4 weight proportion mix homogeneously, packing, sterilization.
Also one or both Chinese medicines in the above prescription can be mixed with different compound blood products with bright Sanguis cervi as stated above, to produce different tonic effects.
Claims (6)
1, a kind of acquisition method of fresh deer blood, it is characterized in that: select the healthy deer that lives, earlier through health check-up and sterilization, again through anesthesia or fixing, the sterilization blood taking bag that connects 400～800ml then with No. 16 blood taking needles of improved lengthening, under aseptic condition, blood taking needle is thrust the deer vena systemica, slowly adopt to prescribed dose with active method or passive means; Take out blood taking needle, preserve or make freeze dried powder under-20 ℃～4 ℃ the temperature and preserve being placed into after the bright Sanguis cervi packing of gathering, the sterilization; Anticoagulant and nutritional solution are arranged in the above-mentioned blood taking bag.
2, according to the acquisition method of the described fresh deer blood of claim 1, it is characterized in that: used anticoagulant is Ca
++Chelating agent.
3, according to the acquisition method of the described fresh deer blood of claim 1, it is characterized in that: used nutritional solution is a glucose.
4, according to the acquisition method of the described fresh deer blood of claim 1, it is characterized in that: blood sampling cycle is 7～15 days, a blood sampling volume≤800m1.
5, according to the acquisition method of the described fresh deer blood of claim 1, it is characterized in that: above-mentioned bright Sanguis cervi adopts ultrasonic sterilization, and frequency is 8～20HZ.
6, a kind of processing method of fresh deer blood is characterized in that: this method comprises separation purification, compatibility Sanguis Anseris domestica or the Chinese medicinal ingredients of fresh deer blood, is specially:
1), the separation of fresh deer blood is purified
The preparation of composition blood: the bright Sanguis cervi that will gather is centrifugal under 4000～5000rpm condition with centrifuge under aseptic condition, 15 minutes, collects blood plasma and cell composition;
Serum preparation: the bright Sanguis cervi that will gather solidified under aseptic condition 12～24 hours, and is centrifugal under 4000～5000rpm condition then, 15 minutes, collects supernatant and gets final product;
2) compatibility Sanguis Anseris domestica or Chinese medicinal ingredients in the fresh deer blood promptly prepare compound blood products:
A, Sanguis cervi and Sanguis Anseris domestica are processed into compound blood products by 1: 0.5～1 (weight proportion), regulate pH value 7.0～7.5;
The soaking in Chinese liquor liquid of b, Sanguis cervi and Chinese herbal medicine is mixed with composite article, and the proportioning of Chinese herbal medicine is (consumption is a weight portion):
Radix Ginseng 1～3 Radix Astragali 5～20 Ganodermas 2～8 Fructus Lyciis 5～20
Said herbal medicine is soaked in the 60 degree Chinese liquor, and the weight proportion of Chinese herbal medicine and Chinese liquor is 1: 5, and soak time is 20 days, will soak filtrate and bright Sanguis cervi mix homogeneously, packing, sterilization then, and the weight proportion of bright Sanguis cervi and Chinese medicine soak solution is 4: 1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN96104430A CN1162450A (en) | 1996-04-18 | 1996-04-18 | Method for collecting and processing fresh deer blood |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN96104430A CN1162450A (en) | 1996-04-18 | 1996-04-18 | Method for collecting and processing fresh deer blood |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1162450A true CN1162450A (en) | 1997-10-22 |
Family
ID=5118348
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN96104430A Pending CN1162450A (en) | 1996-04-18 | 1996-04-18 | Method for collecting and processing fresh deer blood |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1162450A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102719343A (en) * | 2012-05-24 | 2012-10-10 | 华星 | Deer blood wine and preparation method thereof |
| CN104784208A (en) * | 2015-05-11 | 2015-07-22 | 贵州广济堂药业有限公司 | Deer blood crystal body and preparation method thereof |
| CN107258662A (en) * | 2017-05-17 | 2017-10-20 | 安徽禾泉农庄生态农业有限公司 | A kind of cultural method for improving active component in sika deer blood |
| CN111534408A (en) * | 2020-04-26 | 2020-08-14 | 玉树黄河源良种繁育有限公司 | Ancient Tibetan medicine deer blood prepared wine |
-
1996
- 1996-04-18 CN CN96104430A patent/CN1162450A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102719343A (en) * | 2012-05-24 | 2012-10-10 | 华星 | Deer blood wine and preparation method thereof |
| CN102719343B (en) * | 2012-05-24 | 2013-12-11 | 华星 | Deer blood wine and preparation method thereof |
| CN104784208A (en) * | 2015-05-11 | 2015-07-22 | 贵州广济堂药业有限公司 | Deer blood crystal body and preparation method thereof |
| CN107258662A (en) * | 2017-05-17 | 2017-10-20 | 安徽禾泉农庄生态农业有限公司 | A kind of cultural method for improving active component in sika deer blood |
| CN111534408A (en) * | 2020-04-26 | 2020-08-14 | 玉树黄河源良种繁育有限公司 | Ancient Tibetan medicine deer blood prepared wine |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CA2871478C (en) | Pleuropterus multiflorus extract and dipsacus asperoides extract for stimulating the secretion of insulin-like growth factor and promoting bone structure growth, and method for preparing same | |
| RU2061491C1 (en) | Method of preparing agent increasing organism resistance | |
| JP7391204B2 (en) | Extracts of American cockroach, formulations, preparation methods and uses thereof | |
| CN101884703A (en) | Application of medicinal composition in preparation of autoimmune disease treatment medicaments | |
| CN1162450A (en) | Method for collecting and processing fresh deer blood | |
| Adedapo et al. | Evaluation of Telfaria occidentalis and Sorghum bicolor extracts as potent haematinics in domestic rabbits | |
| CN1683522A (en) | Whole blood quality control substance as cell bio-activity protector and its preparing method | |
| EP4523698A1 (en) | Composition of malus niedzwetzkyana dieck and prunus cerasifera ehrhart and use thereof in terms of resisting atherosclerosis | |
| CN100506283C (en) | Plant substrates | |
| CN101617786A (en) | Honey food suitable for diabetes patient and preparation process thereof | |
| CN102335347A (en) | Panax pseudo-ginseng and panax quinquefolium soft capsules | |
| CN1062741C (en) | Animal blood hirudo hydrolytic method and hirudo hydrolytic blood | |
| CN1014484B (en) | Method for extracting bovine colostrum | |
| KR20100101988A (en) | Health care food silkworm hemolymph extract and process for preparing thereof | |
| Irinmwinuwa et al. | Investigation into the effect of ethanol leaf extract and fractions of Justicia Secunda on platelets and WBC counts in mice | |
| CN1884504A (en) | Method for extracting high purity SOD using pure physical method | |
| LU501472B1 (en) | Hypoglycemic Propolis Pollen and Its Preparation Method | |
| KR100335578B1 (en) | Composition for preventing carcinogenesis containing Viscum album extract | |
| CN102274241A (en) | Method for extracting sheep embryo extract and medicinal composition comprising same | |
| CN110327273B (en) | Whitening composition, whitening skin care product and preparation method thereof | |
| Uduchi et al. | Medicinal Effects of Sida acuta Ethanolic Extract on Some Haematological Indices in Rattus albus | |
| Airaodion et al. | Effect of ethanolic extract of Corchorus olitorius leaves on hematological parameters of Wistar rats | |
| Pakaya et al. | The Effect of Giving Extract African Catfish Meat (Clarias Gariepinus) on the Length of Time for Cuts Healing Process among White Male Wistar Rats (Rattus norvegicus) | |
| KR20100070154A (en) | Anti-diabetic composition comprising essentially of chlorella hot extract | |
| CN1121227C (en) | Veterinary medicine prepared from Sophora sophora seed and its production process |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C01 | Deemed withdrawal of patent application (patent law 1993) | ||
| WD01 | Invention patent application deemed withdrawn after publication |