CN116211986A - A Curcumae rhizoma extract, and its preparation method and application - Google Patents
A Curcumae rhizoma extract, and its preparation method and application Download PDFInfo
- Publication number
- CN116211986A CN116211986A CN202310347708.3A CN202310347708A CN116211986A CN 116211986 A CN116211986 A CN 116211986A CN 202310347708 A CN202310347708 A CN 202310347708A CN 116211986 A CN116211986 A CN 116211986A
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- extract
- extraction
- zedoary
- curcuma zedoary
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Links
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Abstract
The invention provides a curcuma zedoary extract and a preparation method and application thereof, wherein the method comprises the following steps: mixing the curcuma zedoary raw material with an extraction solvent to obtain a mixed solution; adding auxiliary extraction substances into the mixed solution to obtain an extraction solution; filtering the extractive solution to obtain Curcumae rhizoma extract fine filtrate. The extract of zedoary turmeric prepared by the method and the application of the extract in preparing skin care products are also provided. The curcuma zedoary extract prepared by the method has high content of active ingredients such as flavone and polyphenol, the extraction rate can be obviously improved, and the cosmetics prepared by the curcuma zedoary extract have instant oil control effect, can effectively reduce sebum secretion level, have instant moisturizing effect, and also have a certain skin barrier repairing effect.
Description
Technical Field
The invention belongs to the technical field of skin care products, and particularly relates to a curcuma zedoary extract and a preparation method and application thereof.
Background
The modern phytochemical research shows that the chemical components of the curcuma zedoary mainly comprise volatile oil, curcuminoids, polysaccharides, sterols, phenolic acids, alkaloids and the like, and the main components of the curcuma zedoary volatile oil comprise curcumenol, curcumadione, germacrone, beta-elemene, curcuminone, curcuma zedoary, furanodiene and other monoterpenes and sesquiterpenoids. Modern pharmacological researches show that the curcuma zedoary has various pharmacological effects of resisting tumor, platelet aggregation, thrombus, blood lipid and atherosclerosis, treating and protecting cerebral apoplexy, resisting tissue fibrosis of liver, kidney and lung, resisting inflammation, easing pain, resisting bacteria, resisting virus, reducing blood sugar, resisting oxidation and the like.
Patent application number CN200710128906.1 discloses a zedoary turmeric oil extract and a preparation method and application thereof, wherein the preparation method comprises the following steps: pulverizing Curcumae rhizoma into 20-40 mesh fine powder, placing into a supercritical extraction apparatus, extracting under 5-18 Mpa at 20-55deg.C, separating at I, II at 7-30 Mpa at 45-70deg.C, and CO2 flow rate of 20-100 kg/h for 2-8 hr to obtain Curcuma rhizome oil extract mainly containing sesquiterpene component, wherein furanodiene + germacrone + curcuminone content in total oil is greater than or equal to 50%, and the patent also discloses application of Curcuma rhizome oil in preparing anticancer drugs. Patent application number CN201410315378.0 discloses a preparation process of a traditional Chinese medicine extract zedoary turmeric oil. The preparation process comprises slicing rhizoma Curcumae, cutting into small particles, extracting volatile oil by steam distillation, and refining volatile oil with ethanol to obtain rhizoma Curcumae oil.
It can be seen that the methods for preparing the curcuma zedoary extract in the prior art mainly comprise supercritical extraction, steam distillation and the like, and the components of the extracts extracted by different methods are completely different, and the application fields of the extracts are also completely different.
In the cosmetic field, the oil control components can be classified into (1) acids such as salicylic acid, azelaic acid, sarcosine, etc., which can accelerate the regeneration of the horny layer of skin, and has the effects of controlling oil and dredging pores. (2) The natural extract such as extracts of herba Salvia officinalis, glycyrrhrizae radix, herba Centellae, etc. can also inhibit oil secretion and repair skin barrier. (3) Other: and nicotinamide, etc. can reduce the generation of fatty acid and triglyceride in sebum, thereby reducing pores. One of the existing oil control technologies is to remove a large amount of grease on the skin surface by a powerful face cleaning method, an oil absorbing paper method or an oil absorbing powder method, which is most common at present, but only can temporarily control the grease on the skin surface. The second existing oil control technology aims at reducing excessive grease flowing on the skin surface by shrinking pores so as to improve the effect of full gloss, but is more likely to cause pore blockage and cause inflammation and acne for skin with too vigorous sebum secretion. The third of the existing oil control technologies is to use zinc salt to inhibit sebum metabolism, but the satisfactory effect cannot be obtained by simply relying on zinc salt, meanwhile, the solubility and the ionic property of zinc salt cause great limitation on the production process of products, and cause a plurality of adverse effects on the products and the stability.
How to prepare the curcuma zedoary extract in the cosmetic field and which method to extract is a new technical problem to be solved urgently.
Disclosure of Invention
The invention aims to solve the technical problems that the curcuma zedoary extract with specific composition is prepared in the field of cosmetics, and the prepared curcuma zedoary extract is applied to the cosmetics, so that the prepared cosmetics have the effects of controlling oil, preserving moisture and repairing.
The technical scheme adopted by the invention is as follows:
the first aspect of the invention provides a preparation method of a curcuma zedoary extract, which comprises the steps of mixing curcuma zedoary raw materials with an extraction solvent to obtain a mixed solution; adding auxiliary extraction substances into the mixed solution to obtain an extraction solution; filtering the extractive solution to obtain Curcumae rhizoma extract fine filtrate.
As a preferred technical scheme, the preparation method of the curcuma zedoary extract comprises the following steps of: (1) (1) pulverizing and sieving the curcuma zedoary raw material, and then adding an extraction solvent to obtain a mixed solution; (2) Adding auxiliary extraction substances into the mixed solution, and soaking at normal temperature; (3) Heating, reflux-extracting and cooling after soaking at normal temperature to obtain an extracting solution; (4) Filtering the extractive solution under reduced pressure to obtain Curcumae rhizoma extract fine filtrate. The extract fine filtrate of zedoary turmeric contains active ingredients such as flavone and polyphenol, wherein the auxiliary extract can cooperate with the extract, so that the content of the flavone and polyphenol in the prepared extract fine filtrate of zedoary turmeric can be remarkably improved.
As a specific technical scheme, the preparation method of the curcuma zedoary extract comprises the following steps of: (1) Pulverizing Curcumae rhizoma, sieving, and adding ethanol water solution (extraction solvent) to obtain mixed solution; (2) Adding auxiliary extraction substances into the mixed solution, and soaking for 1-2 hours at normal temperature; (3) Heating to 60-80deg.C after soaking at normal temperature, reflux extracting, and cooling to below 35deg.C to obtain extractive solution; (4) Filtering the extractive solution under reduced pressure to obtain Curcumae rhizoma extract fine filtrate. In the step (1), the curcuma zedoary raw material is coarsely crushed and then screened, and the mesh number of the screen is generally below 50 meshes, and the screen can be a 10-mesh screen. In the step (2), the soaking time is selected according to the amount of the mixed solution, and the soaking time is 1 to 2 hours, for example, 1 hour, and here, the normal temperature is 25 to 30 ℃. In the step (3), the time of the reflux extraction is generally selected according to the amount of the fed material, and the reflux extraction can be 2 hours as long as the purpose can be achieved.
As a preferred technical scheme, the preparation method further comprises the following steps: mixing the refined filtrate of the curcuma zedoary extract obtained in the step (4) with an organic solvent, concentrating and redissolving to obtain the curcuma zedoary extract. Wherein the organic solvent is butanediol, and the mass ratio of butanediol to Curcumae rhizoma extract fine filtrate is 3-6:1-3, preferably 5:2. In the present invention, the reconstitution means that the mixed concentrated solution is diluted with an organic solvent, and the type of the organic solvent used in the concentration is preferably the same.
As a preferable technical scheme, in the step (1), the extraction solvent is ethanol water solution, and the concentration is 50wt%.
As a preferable technical scheme, in the step (1), the mass ratio of the curcuma zedoary raw material to the extraction solvent is 1:20-1:40. For example, it may be 1:20, 1:25, 1:30, 1:35, 1:40.
As a preferred technical solution, in the step (2), the auxiliary extraction substance is at least one of salicylic acid, zinc gluconate, EDTA-2Na and sodium lactate, and when the auxiliary extraction substance is salicylic acid alone, the concentration thereof may be 0wt% to 0.8wt% (for example, may be 0.2wt%, 0.4wt%, 0.8 wt%) of the extraction solvent, preferably 0.4wt%; when the auxiliary extraction material further comprises zinc gluconate, the addition amount of the zinc gluconate is 0-1.0 wt% of the extraction solvent, preferably 1.0wt%; when the auxiliary extraction substance is a combination of salicylic acid and EDTA-2Na, the concentration of salicylic acid may be 0.4wt% of the extraction solvent, the concentration of EDTA-2Na may be 0.5wt% of the extraction solvent, and when the auxiliary extraction substance is a combination of salicylic acid and sodium lactate, the concentration of salicylic acid may be 0.4wt% of the extraction solvent, and the concentration of sodium lactate may be 1.0wt% of the extraction solvent.
Further preferably, the auxiliary extract consists of salicylic acid and zinc gluconate. Still more preferably, the salicylic acid is added in an amount of 0wt% to 0.8wt% of the extraction solvent (herein, the end point value of 0 is excluded, for example, 0.1wt%, 0.2wt%, 0.3wt%, 0.4wt%, 0.5wt%, 0.6wt%, 0.7wt%, 0.8 wt%), and the zinc gluconate is added in an amount of 0wt% to 1.0wt% of the extraction solvent (herein, the end point value of 0 is excluded, for example, 0.1wt%, 0.2wt%, 0.3wt%, 0.4wt%, 0.5wt%, 0.6wt%, 0.7wt%, 0.8wt%, 0.9wt%, 1.0 wt%) of the flavone and polyphenol content in the extract filtrate of the obtained zedoary turmeric extract and the extraction yield of the two components can be improved simultaneously, and even more preferably, when the salicylic acid is added in an amount of 0.4wt% of the extraction solvent and the zinc gluconate is added in an amount of 1.0wt% of the extraction solvent, the flavone and the polyphenol content in the extract filtrate can be further improved simultaneously.
The second surface of the invention provides the curcuma zedoary extract prepared by the preparation method, wherein the flavone content of the curcuma zedoary extract is 1.3-1.9mg/mL; the polyphenol content is 0.6-1.5mg/mL.
The third aspect of the invention provides application of the curcuma zedoary extract in preparing skin care products, wherein the application is oil control, moisture preservation and restoration.
The method can obviously improve the content of flavone and polyphenol in the curcuma zedoary extract and the extraction rate of the two components, so that the content of the flavone in the prepared curcuma zedoary extract is up to 1.3-1.9mg/mL, and the content of the polyphenol is up to 0.6-1.5mg/mL. Compared with the traditional preparation method of the curcuma zedoary extract, the preparation method of the invention is simple, effective and lower in cost. Furthermore, the curcuma zedoary extract with specific composition prepared by the invention is used for preparing cosmetics, and the cosmetics have the effects of controlling oil, moisturizing and repairing. Namely, the curcuma zedoary extract prepared by the method contains higher content of flavone and polyphenol, and the prepared cosmetics have an instant oil control effect, can effectively reduce sebum secretion level, have an instant moisturizing effect and also have a certain skin barrier repairing effect. The invention adopts a simple, effective and low-cost method to solve the problems of oil control, acne removal, moisture preservation, sensitive muscles and the like which are needed to be solved in the cosmetic field.
Description of the drawings:
fig. 1 is a graph of experimental results of human body efficacy of essence 001, essence 002, essence 003, namely time-controlled oil effect;
fig. 2 shows the results of skin barrier efficacy experiments of essence 001, 002 and 003, wherein the left graph shows the results of skin moisture content and the right graph shows the results of skin moisture loss.
Detailed Description
The following describes specific embodiments of the present invention in detail. It should be understood that the detailed description and specific examples, while indicating and illustrating the invention, are not intended to limit the invention.
Preparation example 1
The preparation example is used for explaining the preparation method of the extract fine filtrate of the curcuma zedoary.
Sieving coarse powder of Curcumae rhizoma with 10 mesh sieve, weighing 75g of pretreated Curcumae rhizoma, adding 1500g of 50% ethanol water solution at a feed-liquid ratio of 1:20 (m/m), adding salicylic acid (0.4wt% of 50% ethanol water solution as extraction solvent), adding zinc gluconate (1.0wt% of 50% ethanol water solution as extraction solvent), soaking at normal temperature for 1 hr, heating to 71 deg.C after soaking at normal temperature, reflux extracting for 2 hr, cooling to below 35deg.C, and filtering under reduced pressure to obtain Curcumae rhizoma extract 001S1 fine filtrate.
Preparation example 2
The preparation example is used for explaining the preparation method of the extract fine filtrate of the curcuma zedoary.
Sieving coarse powder of Curcumae rhizoma with 10 mesh sieve, weighing 75g of pretreated Curcumae rhizoma, adding 1500g of 50% ethanol water solution at a feed-liquid ratio of 1:20 (m/m), adding salicylic acid (the addition amount is 0.4wt% of the extraction solvent 50% ethanol water solution), adding zinc gluconate (the addition amount is 0.25wt% of the extraction solvent 50% ethanol water solution), soaking at normal temperature for 1 hr, heating to 71 deg.C after soaking at normal temperature, reflux extracting for 2 hr, cooling to below 35deg.C, and filtering under reduced pressure to obtain Curcumae rhizoma extract 002S1 refined filtrate.
Preparation example 3
The preparation example is used for explaining the preparation method of the extract fine filtrate of the curcuma zedoary.
Sieving coarse powder of Curcumae rhizoma with 10 mesh sieve, weighing 75g of pretreated Curcumae rhizoma, adding 1500g of 50% ethanol water solution at a feed-liquid ratio of 1:20 (m/m), adding salicylic acid (the addition amount is 0.4wt% of the extraction solvent 50% ethanol water solution), adding zinc gluconate (the addition amount is 0.5wt% of the extraction solvent 50% ethanol water solution), soaking at normal temperature for 1 hr, heating to 71 deg.C after soaking at normal temperature, reflux extracting for 2 hr, cooling to below 35deg.C, and filtering under reduced pressure to obtain Curcumae rhizoma extract 003S1 refined filtrate.
Preparation example 4
The preparation example is used for explaining the preparation method of the extract fine filtrate of the curcuma zedoary.
Sieving coarse powder of Curcumae rhizoma with 10 mesh sieve, weighing 75g of pretreated Curcumae rhizoma, adding 1500g of 50% ethanol water solution at a feed-liquid ratio of 1:20 (m/m), adding salicylic acid (the addition amount is 0.8wt% of the extraction solvent 50% ethanol water solution), soaking at normal temperature for 1 hr, heating to 71 deg.C after soaking at normal temperature, reflux extracting for 2 hr, cooling to below 35deg.C, and filtering under reduced pressure to obtain extract 004S 1.
Preparation example 5
The preparation example is used for explaining the preparation method of the extract fine filtrate of the curcuma zedoary.
Sieving coarse powder of Curcumae rhizoma with 10 mesh sieve, weighing 75g of pretreated Curcumae rhizoma, adding 1500g of 50% ethanol water solution at a feed-liquid ratio of 1:20 (m/m), adding salicylic acid (the addition amount is 0.4wt% of the extraction solvent 50% ethanol water solution), soaking at normal temperature for 1 hr, heating to 71 deg.C after soaking at normal temperature, reflux-extracting for 2 hr, cooling to below 35deg.C, and filtering under reduced pressure to obtain Curcumae rhizoma extract 005S1 refined filtrate.
Preparation example 6
The preparation example is used for explaining the preparation method of the extract fine filtrate of the curcuma zedoary.
Sieving coarse powder of Curcumae rhizoma with 10 mesh sieve, weighing 75g of pretreated Curcumae rhizoma, adding 1500g of 50% ethanol water solution at a feed-liquid ratio of 1:20 (m/m), adding salicylic acid (the addition amount is 0.2wt% of the extraction solvent 50% ethanol water solution), soaking at normal temperature for 1 hr, heating to 71 deg.C after soaking at normal temperature, reflux-extracting for 2 hr, cooling to below 35deg.C, and filtering under reduced pressure to obtain Curcumae rhizoma extract 006S1 refined filtrate.
Preparation example 7
The preparation example is used for explaining the preparation method of the extract fine filtrate of the curcuma zedoary.
Screening coarse powder of rhizoma Curcumae by 10 mesh sieve, weighing 75g of pretreated rhizoma Curcumae, adding 1500g of 50% ethanol water solution according to feed-liquid ratio of 1:20 (m/m), adding salicylic acid (the addition amount is 0.4wt% of the extraction solvent 50% ethanol water solution), adding EDTA-2Na (the addition amount is 0.5wt% of the extraction solvent 50% ethanol water solution), soaking at normal temperature for 1 hr, heating to 71 deg.C after soaking at normal temperature, reflux extracting for 2 hr, cooling to below 35 deg.C, and filtering under reduced pressure to obtain 007S1 fine filtrate of rhizoma Curcumae extract.
Preparation example 8
The preparation example is used for explaining the preparation method of the extract fine filtrate of the curcuma zedoary.
Sieving coarse powder of Curcumae rhizoma with 10 mesh sieve, weighing 75g of pretreated Curcumae rhizoma, adding 1500g of 50% ethanol water solution at a feed-liquid ratio of 1:20 (m/m), adding salicylic acid (0.4wt% of 50% ethanol water solution as extraction solvent), adding sodium lactate (1.0wt% of 50% ethanol water solution as extraction solvent), soaking at normal temperature for 1 hr, heating to 71 deg.C after soaking at normal temperature, reflux extracting for 2 hr, cooling to below 35deg.C, and filtering under reduced pressure to obtain 008S1 refined filtrate of Curcumae rhizoma extract.
Comparative preparation example 1
Sieving coarse powder of Curcumae rhizoma with 10 mesh sieve, weighing 75g of pretreated Curcumae rhizoma, adding 1500g of 50% ethanol water solution at a feed liquid ratio of 1:20 (m/m), soaking at normal temperature for 1 hr, heating to 71 deg.C after soaking at normal temperature, reflux extracting for 2 hr, cooling to below 35deg.C, and filtering under reduced pressure to obtain Curcumae rhizoma extract 009S1 fine filtrate.
Example 1
The zedoary turmeric extract 001S1 fine filtrate prepared in preparation example 1 is put into a rotary steaming bottle, the concentration is reduced by 2-3 times according to the mass of the concentrated solution to the mass of butanediol=5:2 (m: m), the solid content value of the concentrated solution is between 12-15%, and finally the concentrated solution is diluted by 3 times by butanediol, so as to prepare the zedoary turmeric extract 001.
Example 2
The zedoary extract 005S1 fine filtrate obtained in preparation example 5 is put into a rotary steaming bottle, the concentration is reduced by 2-3 times according to the mass of the concentrated solution to the mass of the butanediol=5:2 (m: m), the solid content of the concentrated solution is between 12-15%, and finally the concentrated solution is diluted by 3 times by butanediol to obtain the zedoary extract 005.
Comparative example 1
The zedoary turmeric extract 009S1 fine filtrate prepared in comparative preparation example 1 is put into a rotary steaming bottle, the concentration is reduced by 2-3 times according to the mass of the concentrated solution to the mass of butanediol=5:2 (m: m), the solid content value of the concentrated solution is between 12-15%, and finally the concentrated solution is diluted by 3 times by butanediol, so as to prepare the zedoary turmeric extract 009.
Evaluation of performance:
1. flavone content detection
The flavone detection method comprises the following steps: by NaNO 2 -Al(NO 3 ) 3 Colorimetric determination of the yield of flavonoids in plant extracts (spectrophotometry for determination of total flavonoids in propolis of GB/T20574-2006).
Neutral or weakly alkaline and NaNO 2 Under the existence condition, flavone is reduced, aluminum nitrate is added to complex to form chelate, sodium hydroxide solution is added, under the alkaline condition, flavonoid compound is opened, 2-hydroxy chalcone is generated to display reddish orange, absorption peak is provided at the wavelength of 510nm, and the method accords with beer's law of quantitative analysis, and can be compared with rutin standard series generally for quantitative analysis. Rutin is used as a reference standard in the experiment, naNO is used 2 -Al(NO 3 ) 3 And (3) measuring by a colorimetric method, carrying out linear regression on the absorbance value and the concentration of the rutin solution, solving a regression equation, and obtaining the concentration of the total flavonoids of the object to be measured by the regression equation. The measurement results of preparation examples 1 to 8 and comparative preparation example 1 are shown in Table 1.
TABLE 1 Experimental results of flavone content and flavone extraction yield
The experimental results in table 1 show that: compared with the extract fine filtrate of the zedoary turmeric prepared by extracting the pure ethanol solvent in the comparative preparation example 1, the extraction rate of the flavone in the extract fine filtrate of the zedoary turmeric prepared by the auxiliary extraction system of 0.4 percent salicylic acid and 1.0 percent zinc gluconate in the preparation example 1 of the application is improved by 77.57 percent; compared with the fine filtrate of the zedoary turmeric extract prepared by extraction in preparation example 4 (0.8% salicylic acid), preparation example 5 (0.4% salicylic acid) and preparation example 6 (0.2% salicylic acid), the extraction rate of flavone in the fine filtrate of the zedoary turmeric extract prepared by the auxiliary extraction system of 0.4% salicylic acid and 1.0% zinc gluconate in preparation example 1 of the application is respectively improved by 13.77%, 22.58% and 35.71%; compared with the fine filtrate of the zedoary turmeric extract prepared by extraction in preparation example 7 (0.4% salicylic acid+0.5% EDTA-2 Na) and preparation example 8 (0.4% salicylic acid+1.0% sodium lactate), the extraction rate of flavone in the fine filtrate of the zedoary turmeric extract prepared by the auxiliary extraction system of 0.4% salicylic acid and 1.0% zinc gluconate in preparation example 1 of the application is respectively improved by 30.14% and 40.74%. It can be seen that the salicylic acid and zinc gluconate system have a synergistic effect in terms of the improvement of the flavone extraction rate, and that the synergistic effect is strongest when a 0.4% salicylic acid and 1% zinc gluconate auxiliary extraction system is used.
2. Polyphenol content detection
The polyphenol detection method comprises the following steps: the polyphenol detection method refers to a detection method of the content of tea polyphenol and catechin in GB_T8313-2008 tea leaves. the-OH groups in the polyphenols were oxidized with Fu Lin Fen reagent to give blue color and the polyphenols concentrations were quantified using gallic acid as a calibration standard at a wavelength of 765 nm. The measurement results of preparation examples 1 to 8 and comparative preparation example 1 are shown in Table 2.
TABLE 2 Experimental results of polyphenol content and polyphenol extraction yield
The experimental results in table 2 show that: compared with the extract fine filtrate of the zedoary turmeric prepared by extracting the pure ethanol solvent in the comparative preparation example 1, the extraction rate of polyphenol in the extract fine filtrate of the zedoary turmeric prepared by the auxiliary extraction system of 0.4 percent salicylic acid and 1.0 percent zinc gluconate in the preparation example 1 of the application is improved by 92.24 percent; compared with the fine filtrate of the zedoary turmeric extract prepared by extraction in preparation example 7 (0.4% salicylic acid+0.5% EDTA-2 Na) and preparation example 8 (0.4% salicylic acid+1.0% sodium lactate), the polyphenol extraction rate of the fine filtrate of the zedoary turmeric extract prepared by the auxiliary extraction system of 0.4% salicylic acid and 1.0% zinc gluconate in preparation example 1 of the application is respectively improved by 35.15% and 147%, so that the salicylic acid and zinc gluconate system have a synergistic effect in terms of improving the polyphenol extraction rate, and the synergistic effect is strongest when the auxiliary extraction system of 0.4% salicylic acid and 1% zinc gluconate is adopted.
3. Antioxidation test
The total antioxidant capacity, the capacity of removing 1, 1-Diphenyl-2-trinitrophenylhydrazine free radical (1, 1-Diphenyl-2-picrylhydrazyl, DPPH) and the in-vitro antioxidant effect of the extract fine filtrate and the extract are evaluated. DPPH free radical scavenging test referring to the description of a DPPH free radical scavenging ability kit (built in Nanjing), relevant reagents are prepared: reagent one: throwing the reagent to the bottom before use, and adding 40mL of absolute ethyl alcohol for full dissolution for later use; the used reagent is preserved in dark at 4 ℃. Table 3 shows DPPH test reagent tables.
TABLE 3 DPPH test reagent table
And respectively taking 400 mu L of different sample solutions, placing the sample solutions into a measuring tube, adding the sample according to the method, wherein the absorbance value of the measuring tube is measured by A, the absorbance value of a control tube is controlled by A, and the absorbance value of a blank tube is controlled by A. The clearance and IC50 values of the respective concentrations of the samples were calculated, and the measurement results of preparation examples 1 to 8 and comparative preparation example 1 are shown in table 4. Calculation formula of DPPH free radical scavenging ability:
TABLE 4DPPH radical scavenging Rate (%)
The experimental results in table 4 show that: compared with the pure solvent extraction in comparative preparation example 1, the DPPH clearance of the extract fine filtrate of the zedoary turmeric prepared by adding auxiliary extraction substances in preparation examples 1-8 is obviously improved, wherein the DPPH clearance of the extract fine filtrate of the zedoary turmeric prepared by a system of 0.4wt% salicylic acid and 1.0wt% zinc gluconate in preparation example 1 is the highest.
4. Instant oil control effect human body efficacy test
Human efficacy experiments 5 volunteers (except pregnant or lactating women) aged 18-30 years and having oily skin were selected, and essence 001, essence 002 and essence 003 were prepared respectively, wherein 5% by mass of the extract 009 of zedoary prepared in comparative example 1 was contained in essence 001, 5% by mass of the extract 005 of zedoary prepared in example 5 was contained in essence 002, and 5% by mass of the extract 001 of zedoary prepared in example 1 was contained in essence 003. See table 5 for specific formulations of the essence.
Table 5 essential formulations for testing
Facial cleansing (facial cleansing with alkaline soap-based cleansing product), after cleansing, the skin oil content (within 3 min) was measured and recorded as initial value by blotting with a chipless, water-absorbing dry paper towel. The sample was measured at a concentration of (2.0.+ -. 0.1) mg/cm 2 The amount of the sample was applied in a single pass, quantitatively sampled by a pipette/syringe, uniformly applied to a prescribed area using a latex finger cuff, and the actual applied amount was recorded. Data collection of the oil content was performed in the sample application area and the control area after the set measurement time point of 1h, respectively, wherein the detection index is shown in table 6, and the experimental results of essence 001, essence 002 and essence 003 are shown in fig. 1.
TABLE 6 detection index specification
As can be seen from the results of fig. 1, after 60 minutes of use, the skin oil secretion levels of both essence 002 (containing zedoary extract 005 prepared in example 2) and essence 003 (containing zedoary extract 001 prepared in example 1) were lower than that of the blank; the skin surface sebum secretion was reduced by 17.26% by using essence 003 containing zedoary turmeric extract 001 prepared in example 1, compared to the blank group, indicating that essence 003 containing zedoary turmeric extract 001 prepared in example 1 has an optimal instant oil controlling effect.
5. Skin barrier and skin moisture content human efficacy test
Facial cleansing (facial cleansing with alkaline soap-based cleansing product), after cleansing, the skin oil content (within 3 min) was measured and recorded as initial value by blotting with a chipless, water-absorbing dry paper towel. The sample was measured at a concentration of (2.0.+ -. 0.1) mg/cm 2 The amount of the sample was applied in a single pass, quantitatively sampled by a pipette/syringe, uniformly applied to a prescribed area using a latex finger cuff, and the actual applied amount was recorded. Data collection of skin moisture content and transcutaneous moisture loss (TEWL) were performed in the sample application area and the control area after 30min,1h, respectively, at the set measurement time points, wherein the detection index is shown in table 7, and experimental results of essence 001, essence 002, and essence 003 are shown in fig. 2.
TABLE 7 detection index specification
As can be seen from fig. 2, after 30 minutes of use, the skin moisture content was increased by using the extracts 001, 002 and 003, wherein the skin moisture content was increased most by using the extract 003 containing the extract 001 of zedoary prepared in example 1, indicating that the extract 001 of zedoary prepared in example 1 had the best instant moisturizing effect; meanwhile, the reduction of the skin moisture loss value by using essence 003 containing zedoary turmeric extract 001 prepared in example 1 was the greatest, indicating that zedoary turmeric extract 001 prepared in example 1 has a certain effect of repairing skin barrier.
The preferred embodiments of the present invention have been described in detail above, but the present invention is not limited thereto. Within the scope of the technical idea of the invention, a plurality of simple variants of the technical proposal of the invention can be carried out, comprising that each specific technical feature is combined in any suitable way, and in order to avoid unnecessary repetition, the invention does not need to be additionally described for various possible combinations. Such simple variations and combinations are likewise to be regarded as being within the scope of the present disclosure.
Claims (9)
1. A method for preparing a zedoary turmeric extract, which is characterized by comprising the following steps: mixing the curcuma zedoary raw material with an extraction solvent to obtain a mixed solution; adding auxiliary extraction substances into the mixed solution to obtain an extraction solution; filtering the extract to obtain a fine filtrate of the curcuma zedoary extract.
2. The method of manufacturing according to claim 1, characterized in that the method comprises:
(1) Pulverizing the curcuma zedoary raw material, sieving, and adding the extraction solvent to obtain the mixed solution;
(2) Adding the auxiliary extraction substances into the mixed solution, and then soaking at normal temperature;
(3) Heating, reflux-extracting and cooling after soaking at normal temperature to obtain the extracting solution;
(4) And (3) filtering the extracting solution under reduced pressure to obtain the refined filtrate of the curcuma zedoary extract.
3. The method of manufacturing according to claim 2, characterized in that the method further comprises: mixing the refined filtrate of the curcuma zedoary extract obtained in the step (4) with an organic solvent, concentrating and redissolving to obtain the curcuma zedoary extract.
4. The method according to claim 2, wherein in the step (1), the extraction solvent is an aqueous ethanol solution and the concentration thereof is 50wt%.
5. The preparation method according to claim 2, wherein in the step (1), the mass ratio of the curcuma zedoary raw material to the extraction solvent is 1:20-1:40.
6. The method according to claim 2, wherein in step (2), the auxiliary extraction substance is salicylic acid, and the salicylic acid is added in an amount of 0wt% to 0.8wt%, preferably 0.4wt%, of the extraction solvent.
7. The method according to claim 6, wherein the auxiliary extraction material further comprises zinc gluconate added in an amount of 0wt% to 1.0wt%, preferably 1.0wt%, of the extraction solvent.
8. The zedoary turmeric extract prepared by the preparation method of any one of claims 1-7, wherein the flavone content in the zedoary turmeric extract is 1.3-1.9mg/mL, and the polyphenol content is 0.6-1.5mg/mL.
9. The use of the zedoary turmeric extract as claimed in claim 8 for preparing skin care products, wherein the use is oil control, moisture preservation and repair.
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