CN116179334B

CN116179334B - Saccharomyces cerevisiae culture fermenting installation

Info

Publication number: CN116179334B
Application number: CN202310448825.9A
Authority: CN
Inventors: 梅晓明; 曾贤杰; 王金玲
Original assignee: Shandong Helai Biotechnology Co ltd
Current assignee: Shandong Helai Biotechnology Co ltd
Priority date: 2023-04-24
Filing date: 2023-04-24
Publication date: 2023-06-23
Anticipated expiration: 2043-04-24
Also published as: CN116179334A

Abstract

The invention relates to the field of fermentation devices, in particular to a Saccharomyces cerevisiae culture fermentation device, which comprises a tank body, wherein two fixed blocks are fixedly arranged on the inner side of the tank body, a rotating shaft is rotatably arranged between the two fixed blocks, a rotating plate is fixedly arranged on the outer surface of the rotating shaft corresponding to the side surface of each fixed block, a plurality of through holes are formed in the position, close to the upper end, of the outer surface of the rotating plate, a plugging piece is movably arranged on the inner bottom surface of the tank body along the vertical direction, the plugging piece penetrates through the space between the two fixed blocks, the end part of the plugging piece is contacted with the lower end of the rotating shaft, scraping strips are movably arranged on the position, close to the edge, of the outer surface of the rotating plate, and air injection heads are arranged on the two side surfaces of the fixed blocks.

Description

Saccharomyces cerevisiae culture fermenting installation

Technical Field

The invention relates to the field of fermentation devices, in particular to a saccharomyces cerevisiae culture fermentation device.

Background

Saccharomyces cerevisiae culture is a microecological preparation, and is formed by fully fermenting specific yeast strains according to specific processes and specific culture mediums. The microecological product is different from a product containing active yeast cells, and has the main active ingredients of extracellular metabolites produced by saccharomyces cerevisiae in the fermentation process, and further contains a solid matrix denatured after fermentation, cell contents after wall breaking of the saccharomyces cerevisiae, yeast cell walls and the like, is rich in B vitamins, minerals, small peptides, organic acids, vitamins, nucleotides, mannooligosaccharides, beta-glucan, unknown growth promoting factors and the like, and can be used as a nutrition substrate of probiotics in the gastrointestinal tract of animals, so that the growth of the probiotics can be effectively stimulated, the intestinal flora can be regulated, the utilization rate of the animals on the feed can be effectively improved, and the production performance of the animals can be further improved. It is a microecological feed additive integrating multiple functions of nutrition and health care.

The general production process of yeast culture mainly includes liquid fermentation, in the course of industrial yeast preparation, after the completion of seed culture, firstly inoculating saccharomyces cerevisiae into liquid culture medium to make primary and secondary amplification culture so as to obtain shake flask seed culture solution, then making liquid submerged aerobic fermentation, i.e. inoculating the obtained shake flask seed culture solution into bottom sugar liquid culture medium, in the course of aeration and aerobic fermentation, then adding feeding culture medium to make continuous aerobic fermentation so as to obtain the liquid aerobic fermentation strain liquor of saccharomycetes.

The bottom sugar liquid culture medium provides nutrients, mainly comprises materials such as cane molasses, ammonium sulfate, yeast extract, magnesium sulfate and the like, and has high sugar content, the viscosity of the liquid at the inner side in fermentation is high, the rotating speed of the liquid at the inner side in stirring is 150-200 rpm, the friction heat generation between the liquid and the inner wall in stirring is high due to the high viscosity, the temperature control accuracy is not facilitated, the heating temperature of a heating part is usually lower than the target culture temperature, and a large temperature control error still exists, so that the inconvenience of regulation and control is brought;

due to the existence of sugar substances, more sugar is settled on the inner bottom surface part of the tank body, so that nutrition on the inner side is unevenly distributed, and the fermentation process on the inner side is uneven;

because the inner side is obviously adhered to the inner wall, the stirring part in the tank body is often provided with a scraping structure for scraping the inner wall, and the problem that more substances adhere to the surface of the scraping structure still occurs;

because oxygen is required to be supplied in the culture, the height of the tank body is higher, and the oxygen is difficult to be supplied uniformly at each liquid level;

for the fermentation problems in the prior art, we propose a Saccharomyces cerevisiae culture fermentation device.

Disclosure of Invention

The invention aims to solve the defects in the background art and provides a saccharomyces cerevisiae culture fermentation device.

In order to achieve the above purpose, the invention adopts the following technical scheme: the utility model provides a Saccharomyces cerevisiae culture fermenting installation, includes the jar body, the inboard of jar body is fixed and is provided with two fixed blocks, the inboard of jar body just is located the rotation between two fixed blocks and installs the pivot, the surface of pivot corresponds the side department fixed mounting of every fixed block has the revolving plate, the surface of revolving plate is close to upper end department and has seted up a plurality of through-hole, the interior bottom surface of jar body has the shutoff piece along vertical movable mounting, the shutoff piece passes between two fixed blocks and tip and the lower extreme contact of pivot, the surface of revolving plate is close to the movable mounting of border department and scrapes the strip, the both sides face of fixed block is provided with the air jet head;

when the rotating plate rotates, the liquid on one side forms a pushing area, the extrusion liquid flowing through the through hole falls to the other side of the rotating plate to form a turnover area, and oxygen in the jet head is mixed into the turnover area by the flow of the extrusion liquid.

Preferably, the plugging piece comprises a plugging column which is vertically and slidably inserted into the bottom surface of the tank body, the lower end of the plugging column is fixedly connected with the telescopic end of the telescopic cylinder, the lower end of the telescopic cylinder is fixedly connected with a foot pad, and at least three supporting feet are fixedly arranged on the lower surface of the tank body close to the edge.

Preferably, a top cover is fixedly arranged at the upper end of the tank body, a servo motor is fixedly arranged on the upper surface of the top cover, and an output shaft of the servo motor is fixedly connected to the upper end of the rotating shaft.

Preferably, a rotary cover is rotatably arranged on one side of the top cover, and liquid supplementing ports are fixedly arranged on the upper surface of the rotary cover and the upper surface of the top cover respectively.

Preferably, the upper surface fixed mounting of top cap has the breather pipe, the tip fixedly connected with of breather pipe is divided the pipe, the both ends of being divided the pipe are fixedly connected with intake pipe respectively, the intake pipe runs through the upper surface of top cap and inserts the inboard to the fixed block, the one end of jet head is connected at the surface of intake pipe.

Preferably, a plurality of connecting rods are fixedly connected to one side surface of the scraping strip, the outer surfaces of the connecting rods close to the upper end are sleeved with swinging plates in a common rotating mode, and one ends of the connecting rods are movably connected with the surfaces of the rotating plates through elastic mechanisms.

Preferably, the chamfer has been seted up on the both sides surface of scraping the strip, elastic mechanism includes fixed connection at the connecting seat of rotating plate surface, movable groove has been seted up to the inboard of connecting seat, the connecting rod slip inserts to the movable inslot and at tip fixedly connected with slide bar, slide bar and the inner wall sliding fit of movable groove, the both sides of slide bar are fixedly connected with sleeve respectively, telescopic inboard slip cartridge has the inner tube, inner tube and sleeve prevent to break away from mutually through hanging the mouth, inner tube and telescopic inboard are provided with reset spring.

Preferably, the outer surface of the swinging plate is provided with a rotating opening, the connecting rod penetrates through the inner side of the rotating opening, the upper surface and the lower surface of the connecting rod are respectively fixedly connected with a swinging shaft, and the swinging shafts are in running fit with the inner wall of the rotating opening.

Preferably, the surface fixed mounting of the jar body has a plurality of hot plate, and a plurality of the surface of hot plate covers has the heat insulating shell, the one side surface of the jar body is close to lower extreme department and is provided with the discharge pipe.

Compared with the prior art, the invention has the following beneficial effects:

1. when the rotating plate rotates, liquid on one side forms a pushing area, extrusion liquid flowing through the through hole falls to the other side of the rotating plate to form a turnover area, and the liquid does not generate high-speed friction with the inner wall of the tank body in the pushing process, so that the heat generated by the friction on the inner side is extremely small, and the temperature control is facilitated.

2. At the rotatory in-process of revolving board, the edge of revolving board is clear to the inner wall of jar body and is scraped, and simultaneously, the liquid of extruding in the through-hole has promoted the wobble plate, make the wobble plate rotate as the axle with the pendulum axle, the pendulum board of slope and liquid contact, make the connecting rod by the promotion translation, and then make the scraping strip take place the sideslip, scrape the edge of revolving board, again because reset spring's elasticity effect, make the sliding rod along the unstable removal in the movable tank, the shake number of times of scraping the strip has been increased, and the pendulum plate can just be rotated, by the impact of liquid, irregular swing, make the direction of scraping the strip sideslip change, and then play better cleaning action, prevent the adhesion of viscous substance at the edge of revolving board.

3. Through the mode, the liquid is turned over, in the pushing process, the liquid close to the upper part is extruded out preferentially through the through hole, falls to a lower position when falling into the other side, so that the effect of turning over the liquid is achieved, and the balance of nutrients in the liquid culture medium is facilitated.

4. Oxygen in the jet head is mixed into the overturning area by the flowing of the extrusion liquid flow, so that the oxygen is fully mixed into the liquid, and the balance degree of fermentation is improved.

Drawings

FIG. 1 is a schematic diagram of a fermentation apparatus for Saccharomyces cerevisiae cultures according to the present invention;

FIG. 2 is a cross-sectional view of a Saccharomyces cerevisiae culture fermentation device of the present invention;

FIG. 3 is a schematic view showing the internal partial structure of a fermentation apparatus for Saccharomyces cerevisiae culture of the present invention;

FIG. 4 is a cross-sectional view of a stationary block of a Saccharomyces cerevisiae culture fermentation device of the present invention;

FIG. 5 is a schematic diagram of a rotating plate of a Saccharomyces cerevisiae culture fermentation device of the present invention;

FIG. 6 is an enlarged view of FIG. 5A of a Saccharomyces cerevisiae culture fermentation device of the present invention;

FIG. 7 is a schematic view of a scraper bar of a Saccharomyces cerevisiae culture fermentation device of the present invention;

FIG. 8 is an enlarged view of FIG. 7B of a Saccharomyces cerevisiae culture fermentation device of the present invention;

FIG. 9 is a cross-sectional view of a connection block of a Saccharomyces cerevisiae culture fermentation device of the present invention;

FIG. 10 is a schematic diagram of a Saccharomyces cerevisiae culture fermentation device of the present invention.

1. A tank body; 2. a top cover; 3. a fixed block; 4. a rotating plate; 5. a through hole; 6. a rotating shaft; 7. a servo motor; 9. scraping the strip; 10. chamfering; 11. a connecting rod; 12. a swinging plate; 13. a rotating port; 14. a pendulum shaft; 15. a connecting seat; 16. a movable groove; 17. a slide bar; 18. a sleeve; 19. an inner cylinder; 20. a return spring; 21. hanging the mouth; 22. an air inlet pipe; 23. a jet head; 24. a branch pipe; 25. a vent pipe; 26. a heating plate; 27. a thermal insulation shell; 28. a discharge pipe; 29. supporting feet; 30. foot pads; 31. a telescopic cylinder; 32. plugging the column; 33. a rotary cover; 34. a fluid supplementing port; 35. a pushing zone; 36. a turnover area; 37. extruding a liquid stream.

Detailed Description

The following description is presented to enable one of ordinary skill in the art to make and use the invention. The preferred embodiments in the following description are by way of example only and other obvious variations will occur to those skilled in the art.

The utility model provides a Saccharomyces cerevisiae culture fermenting installation as shown in fig. 1-10, which comprises a tank body 1, two fixed blocks 3 are fixedly arranged on the inner side of the tank body 1, a rotating shaft 6 is rotatably arranged between the two fixed blocks 3 on the inner side of the tank body 1, a rotating plate 4 is fixedly arranged on the outer surface of the rotating shaft 6 corresponding to the side surface of each fixed block 3, a plurality of through holes 5 are formed on the outer surface of the rotating plate 4 near the upper end, a plugging piece is movably arranged on the inner bottom surface of the tank body 1 along the vertical direction, the plugging piece passes through the space between the two fixed blocks 3, the end part of the plugging piece is contacted with the lower end of the rotating shaft 6, a scraping strip 9 is movably arranged on the outer surface of the rotating plate 4 near the edge, and air injection heads 23 are arranged on the two side surfaces of the fixed blocks 3;

when the rotating plate 4 rotates, the liquid on one side forms a pushing area 35, the extrusion liquid flow 37 passing through the through hole 5 falls to the other side of the rotating plate 4 to form a turnover area 36, and oxygen in the jet head 23 is mixed into the turnover area 36 by the flow of the extrusion liquid flow 37.

The invention can be used in aerobic fermentation process in fermentation, changes traditional stirring mode, and solves the problems of temperature rise, insufficient oxygen mixing, adhesion of sticky matters and difficult stirring of sugar.

As shown in fig. 2, the plugging member comprises a plugging column 32 which is vertically and slidably inserted into the inner bottom surface of the tank body 1, the lower end of the plugging column 32 is fixedly connected with the telescopic end of a telescopic cylinder 31, the lower end of the telescopic cylinder 31 is fixedly connected with a foot pad 30, at least three supporting feet 29 are fixedly arranged on the lower surface of the tank body 1 near the edge to ensure the stable placement of the tank body 1, the foot pad 30 increases the stability of the telescopic cylinder 31, the telescopic cylinder 31 is a hydraulic cylinder, the plugging column 32 is controlled to move up and down, after the plugging column 32 moves down, the liquids in two compartments on the inner side of the tank body 1 flow mutually to ensure balance in the liquid, during fermentation, the telescopic cylinder 31 is controlled to move up so as to ensure the pushing action of the swing plate 12, and when the culture liquid is subsequently replenished, the plugging column 32 needs to be controlled to be opened again so that the liquids on two sides are balanced.

The upper end fixed mounting of jar body 1 has top cap 2, and the upper surface fixed mounting of top cap 2 has servo motor 7, and servo motor 7's output shaft fixed connection is in the upper end of pivot 6, drives pivot 6 through servo motor 7 and rotates.

As shown in fig. 1, a rotary cover 33 is rotatably mounted on one side of the top cover 2, and fluid-supplementing ports 34 are fixedly mounted on the upper surface of the rotary cover 33 and the upper surface of the top cover 2, respectively, for supplementing the culture fluid in the later stage or for injecting the seed culture fluid.

As shown in fig. 2, 3 and 4, the ventilation pipe 25 is fixedly mounted on the upper surface of the top cover 2, the end part of the ventilation pipe 25 is fixedly connected with the branch pipes 24, two ends of the branch pipes 24 are respectively fixedly connected with the air inlet pipe 22, the air inlet pipe 22 penetrates through the upper surface of the top cover 2 and is inserted into the inner side of the fixed block 3, and one end of the air jet head 23 is connected to the outer surface of the air inlet pipe 22, so that the functions of oxygen flushing, pressure regulation and the like are realized.

As shown in fig. 5, 6 and 9, a plurality of connecting rods 11 are fixedly connected to one side surface of the scraping strip 9, a swinging plate 12 is sleeved on the outer surface of the connecting rod 11 close to the upper end in a co-rotating mode, and one end of the connecting rod 11 is movably connected with the surface of the rotating plate 4 through an elastic mechanism. The chamfer 10 has been seted up on the both sides surface of scraping strip 9, elastic mechanism includes fixed connection at the connecting seat 15 of swivel plate 4 surface, movable groove 16 has been seted up to the inboard of connecting seat 15, connecting rod 11 slip inserts to movable groove 16 in and at tip fixedly connected with slide bar 17, slide bar 17 and the inner wall sliding fit of movable groove 16, the both sides of slide bar 17 are fixedly connected with sleeve 18 respectively, the inboard slip cartridge of sleeve 18 has inner tube 19, inner tube 19 and sleeve 18 prevent to break away from mutually through hanging 21, hanging 21 is the chimb that sets up at inner tube 19 border, the receipts limit at sleeve 18 border promptly, inner tube 19 and sleeve 18's inboard is provided with reset spring 20.

As shown in fig. 7 and 8, a rotating opening 13 is formed on the outer surface of the swinging plate 12, a connecting rod 11 passes through the inner side of the rotating opening 13, swinging shafts 14 are fixedly connected to the upper and lower surfaces of the connecting rod 11 respectively, the swinging shafts 14 are in rotating fit with the inner wall of the rotating opening 13, and the rotating opening 13 is used for providing space for the rotation of the swinging plate 12.

As shown in fig. 2, the outer surface of the tank 1 is fixedly provided with a plurality of heating plates 26, the outer surface of the plurality of heating plates 26 is covered with a heat insulation shell 27, a discharge pipe 28 is arranged at the position, close to the lower end, of the outer surface of one side of the tank 1 for discharging liquid, and a valve control switch is arranged at the port of the discharge pipe 28, which is not repeated.

In use, after the rotating cover 33 is opened, culture solution can be added into the tank body 1, one of the two fluid supplementing ports 34 is used for adding yeast seed solution, the other is used for later supplementing nutrient solution, the port of the ventilation pipe 25 is connected with oxygen supply equipment for inside oxygen flushing, the control of the inside air pressure, the temperature control, the acid-base regulation and the like are all of the prior art, and are not repeated, in fermentation, the rotating plate 4 is driven by the servo motor 7 to reciprocate positively and negatively, when the rotating plate 4 rotates, one side liquid forms a pushing area 35, the extrusion liquid flow 37 passing through the through hole 5 falls to the other side of the rotating plate 4 to form a turnover area 36, and in the pushing process, the liquid does not generate high-speed friction with the inner wall of the tank body 1, so that the inside friction heat production is very little, and the temperature control is facilitated. In the process of rotating the rotating plate 4, the edge of the rotating plate 4 cleans the inner wall of the tank body 1, meanwhile, liquid extruded in the through hole 5 pushes the swinging plate 12, so that the swinging plate 12 rotates by taking the swinging shaft 14 as an axis, the inclined swinging plate 12 contacts with the liquid, the connecting rod 11 is pushed to translate, the scraping strip 9 moves transversely, the edge of the rotating plate 4 is scraped, the sliding rod 17 moves unstably along the movable groove 16 due to the elastic force of the reset spring 20, the shaking times of the scraping strip 9 are increased, the swinging plate 12 can rotate positively and negatively, the direction of the transverse movement of the scraping strip 9 changes due to the impact of the liquid, and a better cleaning effect is achieved, and the adhesion of viscous substances on the edge of the rotating plate 4 is prevented. Through this mode to liquid turn, in the pushing process, the liquid that is close to the top is extruded through 5 preferentially, falls to comparatively lower position when falling into the opposite side to this plays the effect of turning liquid, does benefit to the equilibrium of nutrient in the liquid culture medium. Oxygen in the jet head 23 is mixed into the turnover zone 36 by the flow of the extrusion liquid flow 37, so that oxygen is fully mixed into the liquid, and the balance degree of fermentation is improved.

The foregoing has shown and described the basic principles, principal features and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, and that the above embodiments and descriptions are merely illustrative of the principles of the present invention, and various changes and modifications may be made therein without departing from the spirit and scope of the invention, which is defined by the appended claims. The scope of the invention is defined by the appended claims and equivalents thereof.

Claims

1. The utility model provides a Saccharomyces cerevisiae culture fermenting installation, includes jar body (1), its characterized in that: the novel tank is characterized in that two fixed blocks (3) are fixedly arranged on the inner side of the tank body (1), a rotating shaft (6) is rotatably arranged between the two fixed blocks (3) on the inner side of the tank body (1), a rotating plate (4) is fixedly arranged on the outer surface of the rotating shaft (6) corresponding to the side face of each fixed block (3), a plurality of through holes (5) are formed in the position, close to the upper end, of the outer surface of the rotating plate (4), a plugging piece is movably arranged on the inner bottom surface of the tank body (1) along the vertical direction, the plugging piece penetrates through the space between the two fixed blocks (3) and the end part of the plugging piece is in contact with the lower end of the rotating shaft (6), a scraping strip (9) is movably arranged on the outer surface, close to the edge, of the rotating plate (4), and jet heads (23) are arranged on the two side faces of the fixed blocks (3);

when the rotating plate (4) rotates, the liquid on one side forms a pushing area (35), the extrusion liquid flow (37) passing through the through hole (5) falls to the other side of the rotating plate (4) to form a turnover area (36), and oxygen in the jet head (23) is mixed into the turnover area (36) by the flow of the extrusion liquid flow (37).

2. A saccharomyces cerevisiae culture fermentation device according to claim 1 wherein: the plugging piece comprises a plugging column (32) which is vertically and slidably inserted into the inner bottom surface of the tank body (1), the lower end of the plugging column (32) is fixedly connected with the telescopic end of the telescopic cylinder (31), the lower end of the telescopic cylinder (31) is fixedly connected with a foot pad (30), and the lower surface of the tank body (1) is fixedly provided with at least three supporting feet (29) close to the edge.

3. A saccharomyces cerevisiae culture fermentation device according to claim 1 wherein: the upper end of the tank body (1) is fixedly provided with a top cover (2), the upper surface of the top cover (2) is fixedly provided with a servo motor (7), and an output shaft of the servo motor (7) is fixedly connected with the upper end of the rotating shaft (6).

4. A saccharomyces cerevisiae culture fermentation device according to claim 3 wherein: one side of the top cover (2) is rotatably provided with a rotary cover (33), and the upper surface of the rotary cover (33) and the upper surface of the top cover (2) are respectively and fixedly provided with a liquid supplementing port (34).

5. A saccharomyces cerevisiae culture fermentation device according to claim 3 wherein: the upper surface fixed mounting of top cap (2) has breather pipe (25), the tip fixedly connected with of breather pipe (25) is in charge of (24), the both ends of being in charge of (24) are fixedly connected with intake pipe (22) respectively, intake pipe (22) run through the upper surface of top cap (2) and insert to the inboard of fixed block (3), the one end of jet head (23) is connected at the surface of intake pipe (22).

6. A saccharomyces cerevisiae culture fermentation device according to claim 1 wherein: one side surface fixedly connected with a plurality of connecting rod (11) of scraping strip (9), the surface joint rotation cover of connecting rod (11) near upper end department is equipped with swing plate (12), the one end of connecting rod (11) is through the surface swing joint of elastic mechanism with rotating plate (4).

7. The Saccharomyces cerevisiae culture fermentation device of claim 6 wherein: the utility model discloses a scraper blade, including scraper blade (9), scraper blade (9) and sleeve (18) are provided with chamfer (10) on the both sides surface, elastic mechanism includes connecting seat (15) of fixed connection at rotating plate (4) surface, movable groove (16) have been seted up to the inboard of connecting seat (15), connecting rod (11) slip inserts in movable groove (16) and at tip fixedly connected with slide bar (17), slide bar (17) and the inner wall sliding fit of movable groove (16), the both sides of slide bar (17) are fixedly connected with sleeve (18) respectively, the inboard slip cartridge of sleeve (18) has inner tube (19), inner tube (19) are prevented breaking away from mutually through hanging mouth (21) with sleeve (18), the inboard of inner tube (19) and sleeve (18) is provided with reset spring (20).

8. The Saccharomyces cerevisiae culture fermentation device of claim 7 wherein: the outer surface of the swinging plate (12) is provided with a rotating opening (13), the connecting rod (11) penetrates through the inner side of the rotating opening (13), the upper surface and the lower surface of the connecting rod (11) are fixedly connected with swinging shafts (14) respectively, and the swinging shafts (14) are in running fit with the inner wall of the rotating opening (13).

9. A saccharomyces cerevisiae culture fermentation device according to claim 1 wherein: the outer surface of the tank body (1) is fixedly provided with a plurality of heating plates (26), the outer surfaces of the heating plates (26) are covered with a heat insulation shell (27), and a discharge pipe (28) is arranged at the position, close to the lower end, of the outer surface of one side of the tank body (1).