CN116179324A - Poultry virus detection device and detection method - Google Patents
Poultry virus detection device and detection method Download PDFInfo
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- CN116179324A CN116179324A CN202211720000.XA CN202211720000A CN116179324A CN 116179324 A CN116179324 A CN 116179324A CN 202211720000 A CN202211720000 A CN 202211720000A CN 116179324 A CN116179324 A CN 116179324A
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- 238000001514 detection method Methods 0.000 title claims abstract description 54
- 244000144977 poultry Species 0.000 title claims abstract description 31
- 241000700605 Viruses Species 0.000 title claims abstract description 29
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 46
- 230000007246 mechanism Effects 0.000 claims abstract description 32
- 238000002360 preparation method Methods 0.000 claims abstract description 25
- 238000003860 storage Methods 0.000 claims abstract description 14
- 238000000034 method Methods 0.000 claims abstract description 5
- 238000000926 separation method Methods 0.000 claims abstract description 5
- 238000009434 installation Methods 0.000 claims description 16
- 239000007788 liquid Substances 0.000 claims description 16
- 238000003825 pressing Methods 0.000 claims description 13
- 235000017166 Bambusa arundinacea Nutrition 0.000 claims description 8
- 235000017491 Bambusa tulda Nutrition 0.000 claims description 8
- 241001330002 Bambuseae Species 0.000 claims description 8
- 235000015334 Phyllostachys viridis Nutrition 0.000 claims description 8
- 239000011425 bamboo Substances 0.000 claims description 8
- 230000000149 penetrating effect Effects 0.000 claims description 6
- 230000008878 coupling Effects 0.000 claims description 3
- 238000010168 coupling process Methods 0.000 claims description 3
- 238000005859 coupling reaction Methods 0.000 claims description 3
- 230000006978 adaptation Effects 0.000 claims description 2
- 238000009826 distribution Methods 0.000 claims 1
- 230000008569 process Effects 0.000 abstract description 2
- 239000000523 sample Substances 0.000 description 47
- 230000006872 improvement Effects 0.000 description 7
- 238000005070 sampling Methods 0.000 description 4
- 230000009471 action Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 244000309464 bull Species 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B02—CRUSHING, PULVERISING, OR DISINTEGRATING; PREPARATORY TREATMENT OF GRAIN FOR MILLING
- B02C—CRUSHING, PULVERISING, OR DISINTEGRATING IN GENERAL; MILLING GRAIN
- B02C18/00—Disintegrating by knives or other cutting or tearing members which chop material into fragments
- B02C18/06—Disintegrating by knives or other cutting or tearing members which chop material into fragments with rotating knives
- B02C18/08—Disintegrating by knives or other cutting or tearing members which chop material into fragments with rotating knives within vertical containers
- B02C18/10—Disintegrating by knives or other cutting or tearing members which chop material into fragments with rotating knives within vertical containers with drive arranged above container
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/70—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in livestock or poultry
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- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Toxicology (AREA)
- Immunology (AREA)
- Microbiology (AREA)
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- Analytical Chemistry (AREA)
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- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Food Science & Technology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention discloses a poultry virus detection device and a detection method, comprising a base, wherein the upper end of the base is fixedly connected with a mounting frame, the top of the mounting frame is provided with an electric telescopic rod, the telescopic end of the electric telescopic rod downwards penetrates through the mounting frame and is fixedly connected with a movable plate, the upper end of the base is provided with a sample preparation cylinder, the lower part of the movable plate is provided with a cylinder cover matched with the sample preparation cylinder, the upper end of the cylinder cover is symmetrically provided with two connecting mechanisms connected with the movable plate, the inside of the cylinder cover is provided with a mounting cavity, the inside of the mounting cavity is provided with a sample preparation mechanism, the side wall of the base is provided with a placing groove, and the inside of the placing groove is provided with a sample storage mechanism; the invention also discloses a poultry virus detection method. The invention can realize a series of continuous operations of sample preparation, sample separation and reagent addition in poultry detection, greatly simplifies the current detection process, does not need to manually add a plurality of reagents in sequence, and effectively improves the efficiency of poultry virus detection.
Description
Technical Field
The invention relates to the technical field of virus detection, in particular to a poultry virus detection device and a poultry virus detection method.
Background
At present, when detecting viruses, tissue is often required to be scattered to prepare a sample, and then a reagent is added into the sample to perform rapid detection.
The Chinese patent comprises a base and a detection box, wherein a detection probe is fixedly installed on the inner wall of the left side of the detection box, a round hole is formed in the top of the detection box, a cylinder is rotatably installed in the round hole, a vertical shaft is rotatably installed in the cylinder, and a first scattering rod is fixedly installed on the outer side of the vertical shaft. In this patent, only design the scheme of system appearance, follow-up minute appearance, sample add reagent detect all do not relate to and improve, and often need the staff to carry out the operation of adding many times in proper order to detect to multiple reagent at present, waste time and energy, and then influence detection efficiency, need improve.
Disclosure of Invention
The invention aims to solve the defects in the prior art and provides a poultry virus detection device and a poultry virus detection method.
In order to achieve the above purpose, the present invention adopts the following technical scheme:
the utility model provides a poultry virus detection device, includes the base, the upper end fixedly connected with mounting bracket of base, electric telescopic handle is installed at the top of mounting bracket, electric telescopic handle's flexible end runs through the mounting bracket downwards and fixedly connected with movable plate, the upper end of base is equipped with a system appearance section of thick bamboo, the below of movable plate is equipped with the cover with system appearance section of thick bamboo adaptation, the upper end symmetry of cover is equipped with two coupling mechanism who is connected with the movable plate, the inside of cover is equipped with the installation cavity, the inside sampling mechanism that is equipped with of installation cavity, be equipped with the standing groove on the lateral wall of base, the inside stock mechanism that is equipped with of standing groove, six play appearance pipes along circumference equidistant connection on the lateral wall that the system appearance section of thick bamboo is close to the lower extreme, go out the upper end that the appearance pipe runs through the base and be located the standing groove, be equipped with the pressing valve on the play appearance pipe, the lower extreme of movable plate is along circumference equidistant fixedly connected with the pressing rod that corresponds with the pressing valve, the upper end of mounting bracket is equipped with six reagent adding mechanism along circumference equidistant.
As a further improvement of the invention, the connecting mechanism comprises a movable rod fixedly connected to the upper end of the cylinder cover, the lower end of the movable plate is provided with a movable groove corresponding to the movable rod, the movable rod is inserted into the movable groove, the upper end of the movable rod is fixedly connected with a spring, and one end of the spring, which is far away from the movable rod, is fixedly connected to the inner top wall of the movable groove.
As a further improvement of the invention, the sampling mechanism comprises a rotating pipe penetrating through the bottom wall in the installation cavity, the rotating pipe is rotationally connected with the bottom wall in the installation cavity, a rotating rod is arranged on the inner side of the rotating pipe, the upper end of the rotating rod is rotationally connected to the inner top wall of the installation cavity, an inner crushing cutter is arranged on the side wall of the rotating rod, two L-shaped rods which are symmetrically arranged are fixedly connected to the side wall of the rotating pipe, outer crushing cutters are arranged on the side wall of the vertical part of the L-shaped rods, the outer crushing cutters and the inner crushing cutters are arranged in a staggered manner, a second bevel gear is fixedly sleeved on the side wall of the rotating pipe, which is positioned in the installation cavity, a first bevel gear is fixedly sleeved on the side wall of the rotating rod, which is positioned above the second bevel gear, a motor is fixedly arranged on the inner side wall of the installation cavity, a third bevel gear is fixedly connected to the tail end of an output shaft of the motor, and the first bevel gear and the second bevel gear are respectively meshed with the upper side and the lower side of the third bevel gear.
As a further improvement of the invention, the reagent adding mechanism comprises a reagent storage tank fixed at the outer top of the mounting frame and an elastic telescopic bag fixed at the inner top of the mounting frame, wherein the elastic telescopic bag is connected with a liquid outlet pipe and a liquid suction pipe, the liquid suction pipe is connected with the reagent storage tank, one end of the liquid outlet pipe, which is far away from the elastic telescopic bag, is connected with a reagent pipe, the reagent pipe penetrates through the upper end of the base and is positioned above the placing groove, and the reagent pipe is fixedly connected with the base.
As a further improvement of the invention, the sample storage mechanism comprises a detection disc arranged in the placing groove, and six sample grooves are arranged at equal intervals along the circumferential direction at the upper end of the detection disc.
As a further improvement of the invention, the liquid outlet pipe is arranged in the installation frame in a penetrating way.
As a further improvement of the invention, the upper end of the base is provided with a positioning bolt in a penetrating way, the positioning bolt is in threaded connection with the base, the positioning bolt extends to the inside of the placing groove, and the lower end of the detection disc is provided with a positioning hole corresponding to the positioning bolt.
As a further improvement of the invention, the bottom of the base is provided with four supporting feet, and the four supporting feet are distributed in a rectangular shape.
A method for detecting poultry viruses, comprising the steps of:
firstly, placing a detection disc in a placing groove, and adding internal tissues of poultry to be detected into a sample preparation cylinder;
secondly, starting the electric telescopic rod to extend, driving the moving plate to move downwards, covering the cylinder cover at the upper end of the sample preparation cylinder through the downward movement of the moving plate, and completing sample preparation through a sample preparation mechanism;
starting the electric telescopic rod to continue to extend, driving the moving plate to continue to move downwards, pressing the pressing valve through the pressing rod, and adding the sample in the sample preparation cylinder into the sample tank through the sample outlet pipe to finish sample separation;
and fourthly, starting the electric telescopic rod to shrink, driving the movable plate to move upwards to the limit position, extruding the elastic telescopic bag through the movable plate, and adding the detection reagent in the elastic telescopic bag into the sample in the sample tank through the reagent tube.
The invention has the beneficial effects that:
1. through setting up movable plate, play appearance pipe, press the valve, press the pole, coupling mechanism, move down through the movable plate, the movable plate drives and presses the pole and press the valve of pressing, adds the sample in the system appearance section of thick bamboo through play appearance pipe in the sample groove, accomplishes the branch appearance, can carry out follow-up branch appearance automatically after the completion of the sample preparation, labour saving and time saving effectively improves the efficiency that poultry virus detected.
2. Through setting up sample storage mechanism, reagent adding mechanism, can once only add the reagent that multiple needs added to the sample tank through reagent adding mechanism, can realize the disposable detection of multiple reagent of sample, greatly simplify present reagent and add the operation, need not the manual work and add multiple reagent in proper order, greatly improve detection efficiency.
The invention can realize a series of continuous operations of sample preparation, sample separation and reagent addition in poultry detection, greatly simplifies the current detection process, does not need to manually add a plurality of reagents in sequence, and effectively improves the efficiency of poultry virus detection.
Drawings
FIG. 1 is a schematic diagram of a poultry virus detection device according to the present invention;
fig. 2 is a schematic top view of a detecting tray and a sample tank of the poultry virus detecting device according to the present invention;
fig. 3 is an enlarged view at a in fig. 1.
In the figure: 1 base, 2 stabilizer blade, 3 standing groove, 4 detection dish, 5 sample groove, 6 locating bolt, 7 locating hole, 8 sampling tube, 9 reagent pipe, 10 press valve, 11 press lever, 12 mounting bracket, 13 drain pipe, 14 movable plate, 15 elastic flexible bag, 16 reagent storage jar, 17 pipette, 18 electric telescopic rod, 19 cover, 20 system appearance section of thick bamboo, 21 bull stick, 22 internal crushing sword, 23L type pole, 24 external crushing sword, 25 movable groove, 26 spring, 27 movable rod, 28 installation chamber, 29 first helical gear, 30 second helical gear, 31 commentaries on classics pipe, 32 third helical gear, 33 motor.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments.
Referring to fig. 1-3, a poultry virus detection device comprises a base 1, four supporting feet 2 are arranged at the bottom of the base 1, the four supporting feet 2 are distributed in a rectangular shape, the upper end of the base 1 is fixedly connected with a mounting frame 12, an electric telescopic rod 18 is arranged at the top of the mounting frame 12, the telescopic end of the electric telescopic rod 18 downwards penetrates through the mounting frame 12 and is fixedly connected with a movable plate 14, a sample preparation cylinder 20 is arranged at the upper end of the base 1, a cylinder cover 19 matched with the sample preparation cylinder 20 is arranged below the movable plate 14, two connecting mechanisms connected with the movable plate 14 are symmetrically arranged at the upper end of the cylinder cover 19, a mounting cavity 28 is arranged in the cylinder cover 19, a sample preparation mechanism is arranged in the mounting cavity 28, a placing groove 3 is arranged on the side wall of the base 1, a sample storage mechanism is arranged in the placing groove 3, a detection disc 4 is arranged in the placing groove 3, the upper end of detecting disk 4 is equipped with six sample grooves 5 along circumference equidistant, after the sample gets into in the sample groove 5 and accomplishes the detection, take out detecting disk 4 look over the result can, the upper end of base 1 runs through and is equipped with positioning bolt 6, positioning bolt 6 and base 1 threaded connection, positioning bolt 6 extends to the inside of standing groove 3, the lower extreme of detecting disk 4 is equipped with the locating hole 7 that corresponds with positioning bolt 6, be close to on the lateral wall of lower extreme along circumference equidistant connection with six play appearance pipes 8 on the lateral wall of a system appearance section of thick bamboo 20, go out the upper end that the appearance pipe 8 runs through base 1 and is located the top of standing groove 3, be equipped with on the play appearance pipe 8 and press valve 10, the lower extreme of movable plate 14 is along circumference equidistant fixedly connected with press the push rod 11 that presses valve 10 corresponds, the upper end of mounting bracket 12 is equipped with six reagent addition mechanisms along circumference equidistant.
In the invention, the connecting mechanism comprises a movable rod 27 fixedly connected to the upper end of the cylinder cover 19, a movable groove 25 corresponding to the movable rod 27 is arranged at the lower end of the movable plate 14, the movable rod 27 is inserted into the movable groove 25, a spring 26 is fixedly connected to the upper end of the movable rod 27, and one end, far away from the movable rod 27, of the spring 26 is fixedly connected to the inner top wall of the movable groove 25.
The concrete sampling mechanism comprises a rotary tube 31 penetrating through the inner bottom wall of the mounting cavity 28, the rotary tube 31 is rotationally connected with the inner bottom wall of the mounting cavity 28, a rotary rod 21 is arranged on the inner side of the rotary tube 31, the upper end of the rotary rod 21 is rotationally connected to the inner top wall of the mounting cavity 28, an inner grinding cutter 22 is arranged on the side wall of the rotary rod 21, two L-shaped rods 23 symmetrically arranged are fixedly connected to the side wall of the rotary tube 31, an outer grinding cutter 24 is arranged on the side wall of the vertical part of the L-shaped rod 23, the outer grinding cutter 24 and the inner grinding cutter 22 are arranged in a staggered mode, a second bevel gear 30 is fixedly sleeved on the side wall of the rotary tube 31 in the mounting cavity 28, a first bevel gear 29 is fixedly sleeved on the side wall of the rotary rod 21 in the mounting cavity 28, the first bevel gear 29 is located above the second bevel gear 30, a motor 33 is fixedly mounted on the inner side wall of the mounting cavity 28, a third bevel gear 32 is fixedly connected to the tail end of an output shaft of the motor 33, the first bevel gear 29 and the second bevel gear 30 are respectively meshed with the upper side and lower sides of the third bevel gear 32, the motor 33 is started, the third bevel gear 32 is driven to rotate, and the first bevel gear 29 and the second bevel gear 30 are respectively meshed with the upper side and lower sides of the third bevel gear 32, the third bevel gear 32 are reversely rotated, and the inner bevel gear 30 is synchronously rotated, and the inner bevel gear 30 is further synchronously and rotated, and the inner bevel gear 32 is reversely, and the inner bevel gear 32 is rotated and ground, and the inner bevel gear 24 is further ground.
The specific reagent adding mechanism comprises a reagent storage tank 16 fixed at the outer top of the mounting frame 12 and an elastic telescopic bag 15 fixed at the inner top of the mounting frame 12, a virus detection reagent is stored in the reagent storage tank 16, a liquid outlet pipe 13 and a liquid suction pipe 17 are connected to the elastic telescopic bag 15, one end, far away from the elastic telescopic bag 15, of the liquid outlet pipe 13 is connected with a reagent pipe 9, check valves are arranged on the liquid outlet pipe 13 and the liquid suction pipe 17, the reagent pipe 9 penetrates through the upper end of the base 1 and is located above the placing groove 3, the reagent pipe 9 is fixedly connected with the base 1, the liquid outlet pipe 13 penetrates through the mounting frame 12, the movable plate 14 moves up to the limiting position, and the elastic telescopic bag 15 is extruded through the movable plate 14, the detection reagent in the elastic telescopic bag 15 can be added into a sample in the sample tank 5 through the reagent pipe 9, and when the movable plate 14 moves down, the elastic telescopic bag 15 stretches and expands under the action of own elasticity, and the actual suction in the reagent storage tank 16 can be temporarily stored in the elastic telescopic bag 15 through the liquid suction pipe 17.
A method for detecting poultry viruses, comprising the steps of:
firstly, placing a detection disc 4 inside a placing groove 3, and adding internal tissues of poultry to be detected into a sample preparation cylinder 20;
secondly, starting the electric telescopic rod 18 to extend, driving the movable plate 14 to move downwards, covering the cylinder cover 19 at the upper end of the sample preparation cylinder 20 through the downward movement of the movable plate 14, and completing sample preparation through a sample preparation mechanism;
thirdly, starting the electric telescopic rod 18 to extend continuously, driving the moving plate 14 to move downwards continuously, pressing the pressing valve 10 through the pressing rod 11, and adding the sample in the sample preparation cylinder 20 into the sample tank 5 through the sample outlet pipe 8 to finish sample separation;
fourth, the electric telescopic rod 18 is started to shrink, the movable plate 14 is driven to move up to the limit position, the elastic telescopic bag 15 is extruded by the movable plate 14, and the detection reagent in the elastic telescopic bag 15 can be added into the sample in the sample tank 5 through the reagent tube 9.
The present invention is not limited to the above-mentioned embodiments, and any person skilled in the art, based on the technical solution of the present invention and the inventive concept thereof, can be replaced or changed within the scope of the present invention.
Claims (9)
1. The utility model provides a poultry virus detection device, includes base (1), its characterized in that, upper end fixedly connected with mounting bracket (12) of base (1), electric telescopic handle (18) are installed at the top of mounting bracket (12), electric telescopic handle (18)'s flexible end runs through mounting bracket (12) downwards and fixedly connected with movable plate (14), the upper end of base (1) is equipped with system appearance section of thick bamboo (20), the below of movable plate (14) is equipped with cover (19) with system appearance section of thick bamboo (20) adaptation, the upper end symmetry of cover (19) is equipped with two coupling mechanism that are connected with movable plate (14), the inside of cover (19) is equipped with installation cavity (28), installation cavity (28) inside is equipped with system prototype structure, be equipped with standing groove (3) on the lateral wall of base (1), the inside sample storage mechanism that is equipped with of standing groove (3), be close to on the lateral wall of lower extreme along circumference equidistant connection six sample tube (8), the upper end (10) are pressed down in the corresponding to be equipped with valve (10) of base (8) along the fixed plate (10), six reagent adding mechanisms are arranged at the upper end of the mounting frame (12) at equal intervals along the circumferential direction.
2. The poultry virus detection device according to claim 1, wherein the connecting mechanism comprises a movable rod (27) fixedly connected to the upper end of the cylinder cover (19), a movable groove (25) corresponding to the movable rod (27) is formed in the lower end of the movable plate (14), the movable rod (27) is inserted into the movable groove (25), a spring (26) is fixedly connected to the upper end of the movable rod (27), and one end, far away from the movable rod (27), of the spring (26) is fixedly connected to the inner top wall of the movable groove (25).
3. The poultry virus detection device according to claim 1, wherein the sample preparation mechanism comprises a rotating tube (31) penetrating through an inner bottom wall of the installation cavity (28), the rotating tube (31) is rotationally connected with an inner bottom wall of the installation cavity (28), a rotating rod (21) is arranged on the inner side of the rotating tube (31), the upper end of the rotating rod (21) is rotationally connected with an inner top wall of the installation cavity (28), an inner crushing cutter (22) is arranged on a side wall of the rotating rod (21), two L-shaped rods (23) which are symmetrically arranged are fixedly connected with the side wall of the rotating tube (31), an outer crushing cutter (24) is arranged on a vertical side wall of the L-shaped rods (23), the outer crushing cutter (24) and the inner crushing cutter (22) are staggered, a second bevel gear (30) is fixedly sleeved on a side wall of the rotating tube (31) in the installation cavity (28), a first bevel gear (29) is fixedly sleeved on a side wall of the rotating rod (21) in the installation cavity (28), two L-shaped rods (23) which are symmetrically arranged are fixedly connected with one another, an outer crushing cutter (24) is fixedly arranged on a side wall of the third bevel gear (33) which is fixedly connected with an output shaft (33) which is fixedly arranged on the third bevel gear (28) The second bevel gear (30) is meshed with the upper side and the lower side of the third bevel gear (32) respectively.
4. The poultry virus detection device according to claim 1, wherein the reagent adding mechanism comprises a reagent storage tank (16) fixed at the outer top of the mounting frame (12) and an elastic telescopic bag (15) fixed at the inner top of the mounting frame (12), a liquid outlet pipe (13) and a liquid suction pipe (17) are connected to the elastic telescopic bag (15), the liquid suction pipe (17) is connected with the reagent storage tank (16), one end, far away from the elastic telescopic bag (15), of the liquid outlet pipe (13) is connected with a reagent pipe (9), the reagent pipe (9) penetrates through the upper end of the base (1) and is located above the placing groove (3), and the reagent pipe (9) is fixedly connected with the base (1).
5. The poultry virus detection device according to claim 1, wherein the sample storage mechanism comprises a detection disc (4) arranged in the placement groove (3), and six sample grooves (5) are formed in the upper end of the detection disc (4) at equal intervals along the circumferential direction.
6. A poultry virus detection device according to claim 4, wherein the outlet pipe (13) is provided through the inside of the mounting frame (12).
7. The poultry virus detection device according to claim 5, wherein a positioning bolt (6) is arranged at the upper end of the base (1) in a penetrating manner, the positioning bolt (6) is in threaded connection with the base (1), the positioning bolt (6) extends into the placing groove (3), and a positioning hole (7) corresponding to the positioning bolt (6) is arranged at the lower end of the detection disc (4).
8. A poultry virus detection device according to claim 1, wherein the bottom of the base (1) is provided with four feet (2), the four feet (2) being rectangular in distribution.
9. A method for detecting a poultry virus comprising the steps of:
firstly, placing a detection disc (4) in a placing groove (3), and adding internal tissues of poultry to be detected into a sample preparation cylinder (20);
secondly, starting the electric telescopic rod (18) to extend, driving the moving plate (14) to move downwards, covering the upper end of the sample preparation barrel (20) by the barrel cover (19) through the downward movement of the moving plate (14), and completing sample preparation through a sample preparation mechanism;
thirdly, starting the electric telescopic rod (18) to extend continuously, driving the moving plate (14) to move downwards continuously, pressing the pressing valve (10) through the pressing rod (11), and adding the sample in the sample preparation barrel (20) into the sample tank (5) through the sample outlet pipe (8) to finish sample separation;
and fourthly, starting the electric telescopic rod (18) to shrink, driving the moving plate (14) to move upwards to the limit position, extruding the elastic telescopic bag (15) through the moving plate (14), and adding the detection reagent in the elastic telescopic bag (15) into the sample in the sample tank (5) through the reagent tube (9).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211720000.XA CN116179324B (en) | 2022-12-30 | 2022-12-30 | Poultry virus detection device and detection method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211720000.XA CN116179324B (en) | 2022-12-30 | 2022-12-30 | Poultry virus detection device and detection method |
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| CN116179324A true CN116179324A (en) | 2023-05-30 |
| CN116179324B CN116179324B (en) | 2024-04-26 |
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| CN202211720000.XA Active CN116179324B (en) | 2022-12-30 | 2022-12-30 | Poultry virus detection device and detection method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117064456A (en) * | 2023-10-17 | 2023-11-17 | 江西省水产科学研究所(江西省鄱阳湖渔业研究中心、江西省渔业资源生态环境监测中心) | Automatic sampling device for crucian immune tissues |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009015290A1 (en) * | 2007-07-24 | 2009-01-29 | Applied Biosystems Inc. | Systems and methods for isolating nucleic acids |
| CN208888208U (en) * | 2018-09-26 | 2019-05-21 | 苏州浩谱嘉自动化装备有限公司 | A kind of Multi-example titration outfit |
| CN113567428A (en) * | 2021-09-23 | 2021-10-29 | 南通恒驰包装机械有限公司 | High-efficient detection device of heavy metal ion in food |
| WO2022041060A1 (en) * | 2020-08-27 | 2022-03-03 | 唐山哈船科技有限公司 | Intelligent port marine environment real-time monitoring device |
| DE202022101882U1 (en) * | 2022-03-16 | 2022-06-07 | Institute Of Animal Science And Veterinary Medicine, Shandong Academy Of Agricultural Sciences | African swine fever virus nucleic acid detection device in ticks |
| CN216879647U (en) * | 2021-11-24 | 2022-07-05 | 天门市金江粮机有限公司 | Biomass particle raw material crushing device |
| CN114848875A (en) * | 2022-04-20 | 2022-08-05 | 信阳农林学院 | Sample collection system is used in poultry virus detection |
| CN217625037U (en) * | 2022-06-17 | 2022-10-21 | 鲁渝农牧科技重庆有限公司 | Poultry infectious diarrhea rapid detection kit device |
| CA3218446A1 (en) * | 2021-05-12 | 2022-11-17 | Xiang Li | Sample processing apparatus and sample processing method |
-
2022
- 2022-12-30 CN CN202211720000.XA patent/CN116179324B/en active Active
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009015290A1 (en) * | 2007-07-24 | 2009-01-29 | Applied Biosystems Inc. | Systems and methods for isolating nucleic acids |
| CN208888208U (en) * | 2018-09-26 | 2019-05-21 | 苏州浩谱嘉自动化装备有限公司 | A kind of Multi-example titration outfit |
| WO2022041060A1 (en) * | 2020-08-27 | 2022-03-03 | 唐山哈船科技有限公司 | Intelligent port marine environment real-time monitoring device |
| CA3218446A1 (en) * | 2021-05-12 | 2022-11-17 | Xiang Li | Sample processing apparatus and sample processing method |
| CN113567428A (en) * | 2021-09-23 | 2021-10-29 | 南通恒驰包装机械有限公司 | High-efficient detection device of heavy metal ion in food |
| CN216879647U (en) * | 2021-11-24 | 2022-07-05 | 天门市金江粮机有限公司 | Biomass particle raw material crushing device |
| DE202022101882U1 (en) * | 2022-03-16 | 2022-06-07 | Institute Of Animal Science And Veterinary Medicine, Shandong Academy Of Agricultural Sciences | African swine fever virus nucleic acid detection device in ticks |
| CN114848875A (en) * | 2022-04-20 | 2022-08-05 | 信阳农林学院 | Sample collection system is used in poultry virus detection |
| CN217625037U (en) * | 2022-06-17 | 2022-10-21 | 鲁渝农牧科技重庆有限公司 | Poultry infectious diarrhea rapid detection kit device |
Non-Patent Citations (1)
| Title |
|---|
| 赵玉军 北京：中国轻工业出版社: "国家法定禽病诊断与防制", pages: 51 - 52 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117064456A (en) * | 2023-10-17 | 2023-11-17 | 江西省水产科学研究所(江西省鄱阳湖渔业研究中心、江西省渔业资源生态环境监测中心) | Automatic sampling device for crucian immune tissues |
| CN117064456B (en) * | 2023-10-17 | 2024-02-02 | 江西省水产科学研究所(江西省鄱阳湖渔业研究中心、江西省渔业资源生态环境监测中心) | Automatic sampling device for crucian immune tissues |
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