CN116171757A

CN116171757A - Method for preventing and controlling red-neck longhorn beetles by leaching spray bars with slow-release medicament

Info

Publication number: CN116171757A
Application number: CN202310039501.XA
Authority: CN
Inventors: 刘锦; 迟宝杰; 束靖; 张大鹏; 刘云; 王功帅
Original assignee: Shandong Agriculture and Engineering University
Current assignee: Shandong Agriculture and Engineering University
Priority date: 2023-01-13
Filing date: 2023-01-13
Publication date: 2023-05-30

Abstract

The invention discloses a method for preventing and controlling red neck longicorn by leaching spray bars with slow release agents, which comprises the following steps: by utilizing chemotaxis of the red neck longhorn beetles, the adults are trapped in the eclosion period of the red neck longhorn beetles, after the adults are trapped (40 days from the start to the end of the trapping, and 7-10 days after the trapping is completed), medicines are sprayed on spawning parts of the red neck longhorn beetles in a leaching manner, 2000 times of 5.7% emamectin benzoate microcapsule liquid, 2000 times of 8% cypermethrin microcapsule liquid and 1500 times of 3% thiacloprid microcapsule liquid are sprayed in sequence at intervals, and the uniform medicine application at the gaps of trunk and main branches of peach trees is ensured. The method for preventing and controlling the red neck longicorn beetles can efficiently kill eggs laid by adults and hatched larvae, is accurate in application, kills the larvae before the larvae dig into deep xylem, can be used for preventing and controlling other diseases and insect pests of peach trees, is simple, convenient and easy to implement, is accurate and efficient, can continuously control harm, and has a prevention and control effect of the red neck longicorn beetles up to 96.7%.

Description

Method for preventing and controlling red-neck longhorn beetles by leaching spray bars with slow-release medicament

Technical Field

The invention relates to the technical field of pest chemical control, in particular to a method for controlling longicorn by leaching spray bars with a slow-release agent.

Background

Red neck longicorn (Aromia bungii Fald), also known as peach red neck longicorn, belongs to the family coleoptera longicorn. The pesticide is widely distributed in China, mainly comprises stone fruit type fruit trees such as peach, apricot, cherry, plum and the like, and is a serious pest in the fruit tree industry in China. The red neck longicorn larva is used for drilling and damaging the trunk in the early period of damage, causing gummosis and diseases, causing the tree vigor to be weakened, and the trunk is damaged and withered in the later period.

Specifically, red neck longicorn completes 1 generation in Shandong and the like for 2 years, spans 3 years, and winters with larvae of different ages in trunk borers for 10 to 11 months. The third year of larva is harmful to the beginning of pupation in the late 5 months, adults emerge in the middle of June, and the appearance time of adults is mainly concentrated in the period from the late June to the early June. Adults find their way out and feed, and find mating and breeding. Female adults lay eggs on the trunk and main branches of peach trees and are scattered and laid in the thick skin seams of the branches. The hatched larvae eat the bark layers of the trunks under the cortex, and eat the trunks in the longitudinal and transverse directions after the larvae are slightly larger, so that irregular tunnels are formed. As the tunnel gradually deepens as the insect grows, young larvae live through winter in the moth. The larvae recover to eat in the 3 months of the next year, and continue eating the xylem until the winter in the trunk. The old larva starts to eat continuously in the third year, pupate in the xylem worm channel for 5-6 months, and adults emerge for 6-7 months, mate and spawn. The adults stop on the trunk and fly away when being disturbed. The adults have chemotaxis and have the tendency to nectar, honey, sugar-vinegar liquid and the like. The adults are subjected to multiple mating among branches, and the spawning period is 7-10 days. Hatching to form larva after about 10 days in egg stage.

The peach tree planted for 1-2 years is not damaged by the red neck longhorn beetles, the young red neck longhorn beetles of the 3-year-old peach tree are damaged by the trunk, the damaged plant rate of the peach tree is obviously increased along with the increase of the tree age, the damaged plant rate of the peach tree for 5-10 years can reach 28.6-43.2%, the damaged plant rate of the peach tree for 11-15 years can reach 48.4-81.7%, the damaged plant rate of the peach tree for more than 15 years is higher, and the death is almost related to the damage of the red neck longhorn beetles. Meanwhile, along with the increase of the tree age, the number of the red neck longicorn larvae on the single peach tree is also obviously increased, and the damage is continuously aggravated. And (3) discharging the reddish brown sawdust-like insect manure outwards from the trunk surface crack gap in the later period, and accumulating the reddish brown sawdust-like insect manure on the trunk base.

The red neck longhorn beetles have long life cycle and the larvae span 3 years. The red neck longhorn beetle is more complex than other trunk borers in that the red neck longhorn beetle is eaten by the worm, and the larvae and the pupae are all crossed in the trunk borers, so that living places are very hidden. The early-stage feces of the larvae are left in the moth way and are not discharged outwards, so that early-stage hazard conditions are not easy to find, and favorable prevention and control time is lost. In the later period, the wood chip-shaped insect manure is discharged from the gap between the worm-eating passage and the bark because of the dry crack of the insect manure and the bark filled in the worm-eating passage, and the insect manure is seriously damaged, cannot be saved and has no control significance. The adult red-neck longhorn beetles emerge in the middle and late June to the upper June of each year, drill foraging and mating propagation from the insect channel, mainly lay eggs on the trunk and main branches of peach trees. Chemotaxis, relatively concentrated eclosion spawning time and spawning sites of the adult peach red neck longicorn are provided for accurate prevention and control.

Therefore, research on the red neck longhorn beetles in peach trees, especially eggs and larvae of red neck longhorn beetles, has positive significance to the technical field of pest control.

Disclosure of Invention

Aiming at the prior art, the invention aims to provide a method for preventing and controlling longicorn by leaching spray bars with slow release agents. The method adopts the slow-release medicament to wash the spray boom to prevent and treat the red-neck longicorn, can efficiently and accurately kill eggs and larvae of the red-neck longicorn, prevents the larvae from drilling in, and has the prevention and treatment rate of 96.7 percent on the red-neck longicorn.

In order to achieve the above purpose, the invention adopts the following technical scheme:

the invention provides a method for preventing and controlling red neck longicorn by slowly eluting spray bars with a slow release agent, which comprises the following steps:

(1) When the adult red-neck longicorn is eclosion, the adult red-neck longicorn is trapped;

(2) After the trapped adult red neck longicorn, sequentially spraying the eggs of the red neck longicorn with 2000 times of 5.7% emamectin benzoate microcapsule, 2000 times of 8% cypermethrin microcapsule and 1500 times of 3% thiacloprid microcapsule at intervals.

Preferably, in the step (1), the method for trapping the adult red neck longicorn beetles comprises the following steps: and hanging sugar-vinegar liquid traps at the middle part of the crown of the peach tree, wherein 1-2 sugar-vinegar liquid traps are hung per mu, and the sugar-vinegar liquid is replaced once in 15-20 days.

More preferably, the peach tree has a tree age of 3 years or more.

Further preferably, the sweet and sour liquid is prepared by mixing sugar, vinegar, wine and water according to a mass ratio of 1:1.5:0.5:18.

Further preferably, the suspension height of the sugar-vinegar trap is 1.5m.

Preferably, in the step (2), the spraying time of the emamectin benzoate microcapsule is 7-10 days after the trapped adult red neck longicorn beetles.

Preferably, in the step (2), the spraying interval of the cypermethrin microcapsule and the emamectin benzoate microcapsule is 15-20 days.

Preferably, in the step (2), the thiacloprid microcapsule and the cypermethrin microcapsule are sprayed for 15-20 days.

Preferably, in the step (2), the 3 spraying times can be combined with the prevention and treatment of other diseases and insect pests to spray the pesticide, generally, independent spraying is not needed, and other efficient pesticides can be selected for use.

Preferably, in the step (2), the leaching type spraying method comprises the following steps: the preparation is uniformly sprayed on the trunk and the main branch of the trunk, and the inside of the bark gaps at the trunk and the main branch are uniformly wetted with the preparation.

The invention has the beneficial effects that:

1. the invention adopts microcapsule technology to spray the medicament on the trunk and main branch of the trunk of peach tree, wherein the microcapsule technology has the functions of enhancing the stability of the medicament, controlling the release of the core material, reducing the volatilization of the active ingredients, and the like, and the pesticide microcapsule is prepared by coating the active ingredients in the wall material, so that the core material medicament maintains a certain effective concentration and is slowly released into the environment within a certain time. The microcapsule not only can improve the physical and chemical stability of the original drug, slowly release the capsule core drug, prolong the pesticide duration, improve the pesticide utilization rate, but also is safe to people and animals and environment-friendly.

2. According to the invention, the appearance time of adults is monitored through sweet and sour liquid trapping, and aiming at the concentrated spawning positions of the adults, the high-efficiency slow-release microcapsule is selected, the main trunk and the main branch are sprayed with the drip-type pesticide, eggs and hatched larvae are killed in time, and the high-efficiency and accurate prevention and control of the red neck longicorn beetles are realized.

3. The invention adopts emamectin benzoate, cypermethrin and thiacloprid as the spraying agents, and can obviously improve the control effect of the red neck longicorn by spraying the three agents at intervals in sequence, and can lead the control effect of the red neck longicorn to reach more than 96.7 percent by combining a leaching type spraying mode. Meanwhile, the pesticide can be sprayed together with other pest control agents in the three pesticide spraying time, the pesticide for controlling other pest control agents does not need to be sprayed independently, and the pesticide can be carried out together with other pest control agents for controlling peach trees in the period of emergence and spawning of the red neck longhorns from the late June to the mid-late June each year, and is simple, convenient, easy, accurate and efficient.

4. The invention adopts the drip washing type spraying agent to accurately apply the agent, the agent is evenly sprayed on the trunk and the main branch of the trunk, and the gaps inside the bark at the trunk and the main branch are evenly wetted with the agent, thereby killing eggs produced by adults and young larvae after hatching, preventing the damage of the young worms into deep xylem and achieving the aim of accurate and efficient prevention and control.

Drawings

Fig. 1: dynamic diagram of adult red neck longicorn;

fig. 2: adult red neck longicorn beetles and larvae;

fig. 3: the red neck longicorn larva is a harmful trunk;

fig. 4: a red neck longicorn trap;

fig. 5: the sweet and sour trap is used for trapping adult red neck longhorn beetles.

Detailed Description

It should be noted that the following detailed description is illustrative and is intended to provide further explanation of the present application. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs.

As described in the background art, the damage of the red-neck longicorn to the trunk of the peach tree with the age of more than or equal to 3 years is improved, and the damage rate of the peach tree is obviously increased along with the increase of the age of the tree.

In order to enable those skilled in the art to more clearly understand the technical solutions of the present application, the technical solutions of the present application will be described in detail below with reference to specific embodiments.

The test materials used in the examples of the present invention are all conventional in the art and are commercially available.

Wherein, 5.7% emamectin benzoate microcapsule suspending agent is produced by Hebei Jiu Biotechnology Co., ltd, 8% cypermethrin microcapsule suspending agent is produced by Chongqing Zhongbang pharmaceutical industry Co., ltd, and 3% thiacloprid microcapsule suspending agent is produced by Jiangsu Chengsu Biotechnology Co., ltd. The control agent 5.7% emamectin benzoate suspending agent is produced by Henan Ai Linong agricultural technologies, inc., 10% cypermethrin emulsifiable concentrate is produced by Jiangsu lan Feng biochemical Co., ltd, and 3% thiacloprid suspending agent is produced by Jiangxi Tian people ecological Co., ltd.

Example 1: dynamic generation of adult red neck longicorn

The Shandong Qufu uguo fruit farm development limited company peach garden base area is 260 mu, and is planted in 2016, and 30 or more early, middle and late maturing varieties such as bright moon, brocade spring, beautiful, golden autumn red honey and the like are available. 3 areas with a mutual interval of 40m are selected in the garden, 5 sugar-vinegar liquid traps are 1 group, the distance between the traps is 20m, 3 groups of 15 traps are suspended, 1-2 traps are suspended per mu, the sugar-vinegar liquid suspension height is changed once for 15-20 days, and the sugar-vinegar liquid traps are placed at a ventilation and light transmission place. The sugar-vinegar liquid comprises the following components: vinegar: wine: water 1.0:1.5:0.5:18. hanging in the middle of 6 months every year, checking and counting the number of the trapped adults every 10 days, and fishing out. The number and time from 2018 to 2022 of the trap to adults was counted and the results are shown in fig. 1.

As can be seen from FIG. 1, the number of the trapping agents tends to increase year by year in the period from the last ten days of 6 months to the last ten days of 7 months, mainly in the peak period from the last ten days of 6 months to the last ten days of 7 months.

Example 2: survey results of spawning sites of adult red neck longicorn

Selecting tree with 4-7 years old, randomly selecting 30 tree, and fixing the spawning number on the main survey trunk and all main branches.

Specifically, the number of eggs laid on the trunk and main branch parts was examined every 7 days from the time of attracting and collecting adults with sugar and vinegar solution, and then eggs were cleared until the time of 30 days after the time of not attracting and collecting adults, and the results are shown in table 1.

TABLE 1 egg laying number of adult Monochamus alternatus in trunk and main branches of peach tree

As can be seen from Table 1, the adult Monochamus alternatus had 86.9% eggs laid on the trunk, 60.5% in 40cm above ground, and 13.1% on the main branches.

Example 3: effect of different concentration of chemical spray boom in preventing and treating red neck longhorn beetles

The red neck longhorn beetles do not harm 1-2 years of peach trees basically, and sugar-vinegar liquid traps are hung in peach gardens in the last ten days of 6 months of year from the 3 rd year of planting. The first time of medicament is sprayed 9 days after the adult red neck longhorn beetles are trapped, the second time of medicament is sprayed at intervals of 16 days, and the third time of medicament is sprayed at intervals of 20 days. Wherein, the spraying agent is 5.7% emamectin benzoate slow-release microcapsule and non-slow release agent, and the concentration of the spraying agent is 1000 times of liquid, 1500 times of liquid and 2000 times of liquid; the concentration of the 8% high-efficiency cypermethrin micro-slow release microcapsule and the non-slow release agent is 1000 times of liquid, 2000 times of liquid and 3000 times of liquid, and the concentration of the 3% thiacloprid micro-slow release microcapsule and the non-slow release agent is 1000 times of liquid, 1500 times of liquid and 2000 times of liquid.

The combination of 3 spray concentrations of these three agents is shown in table 2.

The peach tree with the trunk and the main branch sprayed with the same amount of clean water is used as a control. Each concentration was sprayed 30 times, and 1 time was investigated at 15-20 days after spraying, and whether fresh manure holes appeared in the trunk and main branch portions was investigated plant by plant to calculate the rate of damage of peach trees, and the results are shown in table 2.

Table 2 effects of peach trunk spray on controlling red neck longhorn beetles

According to the pesticide application principle, the same orchard or crops are not allowed to be sprayed with the same pesticide for 3 times in the same year, and the drug resistance can be generated for at most 2 times. Thus, a combination of a sustained release capsule and a non-sustained release agent was compared. As can be seen from the damage rate of peach trees in Table 2, the slow-release microcapsule is selected, and the slow-release microcapsule is used for covering the whole adult emergence period by 3 times of spraying, killing eggs and initially hatched larvae under the adult yield, thoroughly preventing the larvae from penetrating into the trunk, realizing precise control, and having good control effect. And the spraying time is basically consistent with the conventional use of the medicine in the orchard.

Simultaneously, 1000 times of the 5.7% emamectin benzoate microcapsule suspending agent, 1000 times of the 8% cypermethrin microcapsule suspending agent and 1000 times of the 3% thiacloprid microcapsule suspending agent are sequentially sprayed, and 2000 times of the three agents, 2000 times of the solution and 1500 times of the solution are combined, the accumulated damage rate of peach trees in 5 years is 3.3%, and the control effect reaches 96.7%. The three medicaments are combined by 3000 times liquid, 3000 times liquid and 2000 times liquid, the accumulated damage rate of peach trees in 5 years is 13.3 percent, the prevention and control effect is 86.7 percent, and the medicament concentration is low. The non-slow release dosage forms of the three medicaments have the maximum use concentration of 1000 times of liquid, 1000 times of liquid and 1000 times of liquid combination, the accumulated damage rate of peach trees in 5 years is 20.0 percent, the prevention and control effect is 80.0 percent, and the highest concentration is selected, so that the medicament dosage form has no slow release effect, the effective period is short and only about 10 days, the next spraying time can not be continuously controlled, and some hatching larvae can be drilled into the trunk to survive while the hatching larvae are still in the trunk. The prevention and control effect and the cost input are comprehensively considered, and the three medicaments are selected from a combination of 2000 times of liquid, 2000 times of liquid and 1500 times of liquid, so that the effect of effectively preventing and controlling the damage of the red neck longicorn can be achieved.

Example 4: medicament leaching spray boom for preventing and treating red neck longhorn beetles

(1) When the adult red neck longicorn beetles emerged, the adult red neck longicorn beetles were trapped in the same manner as in example 1;

(2) After the red neck longhorn beetles are trapped, 10 days later, 5.7% emamectin benzoate microcapsule 2000-fold liquid is uniformly sprayed on the trunk and the main branches of the trunk of the peach tree, and the gaps between the trunk and the bark of the main branches are uniformly sprayed with 8% cypermethrin microcapsule 2000-fold liquid 15 days apart, and 3% thiacloprid microcapsule 1500-fold liquid 15 days apart.

Example 5: medicament leaching spray boom for preventing and treating red neck longhorn beetles

(2) After the adult red neck longhorn beetles are trapped, 5.7% emamectin benzoate microcapsule 2000-fold liquid is uniformly sprayed on the trunk and the main branches of the trunk of the peach tree, and the gaps between the trunk and the bark of the main branches are uniformly sprayed with 8% cypermethrin microcapsule 2000-fold liquid at intervals of 20 days, and 3% thiacloprid microcapsule 1500-fold liquid at intervals of 20 days.

Example 6: medicament leaching spray boom for preventing and treating red neck longhorn beetles

(2) After the adult red neck longhorn beetles are trapped, 5.7% emamectin benzoate microcapsule 2000-fold liquid is uniformly sprayed on the trunk and the main branches of the trunk of the peach tree, and the gaps between the trunk and the bark of the main branches are uniformly sprayed with 8% cypermethrin microcapsule 2000-fold liquid at intervals of 17 days, and 3% thiacloprid microcapsule 1500-fold liquid at intervals of 17 days.

Examples 4, 5 and 6 were conducted with the trunk and main branches not sprayed with the pesticide, and with the same amount of fresh water sprayed on peach trees as a control.

Table 3 effects of peach trunk slow-release medicament spray on preventing and controlling red neck longhorn beetles

As can be seen from the damage rate of peach trees in Table 3, after the adult red neck longhorn beetles are trapped, 2000 times of 5.7% emamectin benzoate microcapsule suspending agent is sprayed on 10 days, 7 days and 8 days respectively, 2000 times of 8% cypermethrin microcapsule suspending agent is sprayed on 15 days, 20 days and 17 days respectively after the first spraying, 1500 times of 3% thiacloprid microcapsule suspending agent is sprayed on 15 days, 20 days and 17 days respectively after the second spraying, and the cumulative damage rate of the peach trees in 5 years is 3.3%, 0 and 0, and the prevention and control effect reaches over 96.7%. The first medicine is sprayed 7-10 days after adult induction, 15-20 days after the first medicine spraying and the third medicine is sprayed 15-20 days after the second medicine spraying, and good effects can be achieved.

Comparative example 1: effect of preventing and controlling red neck longhorn beetles by adopting emamectin benzoate and cypermethrin combination

(2) Spraying a first medicine 9 days after the adult red neck longhorn beetles are trapped, spraying a second medicine 20 days after the first medicine spraying, and uniformly spraying 5.7% emamectin benzoate microcapsule suspending agent and 8% cypermethrin microcapsule suspending agent in gaps of the trunk and the main branches of peach trees, wherein the 1000 times liquid and 1000 times liquid combination, the 2000 times liquid and 2000 times liquid combination and the 3000 times liquid and 3000 times liquid combination.

Comparative example 2: effect of preventing and controlling red neck longhorn beetles by adopting emamectin benzoate and thiacloprid combination

(2) Spraying a first medicine 9 days after the adult red neck longhorn beetles are trapped, spraying a second medicine 20 days after the first medicine spraying, and uniformly spraying 5.7% emamectin benzoate microcapsule suspending agent and 3% thiacloprid microcapsule suspending agent in the gaps of the trunk and the main branches of the peach tree, wherein the 1000 times of liquid and 1000 times of liquid combination, the 2000 times of liquid and 1500 times of liquid combination and the 3000 times of liquid and 2000 times of liquid combination.

Comparative example 3: effect of preventing and controlling red neck longhorn beetles by adopting combination of cypermethrin and thiacloprid

(2) Spraying a first medicine 9 days after the adult red neck longhorn beetles are trapped, spraying a second medicine 20 days after the first medicine spraying, and uniformly spraying 8% cypermethrin microcapsule suspending agent and 3% thiacloprid microcapsule suspending agent in gaps of the trunk and the main branches of peach trees, wherein the 1000 times of liquid and 1000 times of liquid combination, the 2000 times of liquid and 1500 times of liquid combination and the 3000 times of liquid and 2000 times of liquid combination.

Comparative examples 1, 2 and 3 were conducted with the trunk and main branches not sprayed with the pesticide, and with the same amount of fresh water sprayed on peach trees as a control. TABLE 4 Effect of peach tree spray agent on controlling red neck longhorn beetles

Comparative examples 1 to 3 are combinations of two pesticides, and the damage rate of peach trees in comparative examples 1 to 3 and examples 3 to 6 is examined, wherein the combination of 2000 times of 5.7% emamectin benzoate microcapsule suspending agent, 2000 times of 8% cypermethrin microcapsule suspending agent and 1500 times of 3% thiacloprid microcapsule suspending agent in examples 3 to 6 shows that the control rate of the red neck longhorn beetles can reach 96.7% or more, and the three pesticide combinations have better control effect on the red neck longhorn beetles. The two pesticides are combined for application, the effective control time cannot cover the egg laying duration and the larva hatching time of the adult red neck longicorn beetles (from the middle ten days of 6 months to the late 7 months), and the three pesticides are combined for application, so that the effective control time can cover the egg laying duration and the larva hatching time of the adult red neck longicorn beetles, and the control fruit is better.

The foregoing description is only of the preferred embodiments of the present application and is not intended to limit the same, but rather, various modifications and variations may be made by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principles of the present application should be included in the protection scope of the present application.

Claims

1. The method for preventing and controlling red neck longhorn beetles by leaching spray bars with slow release medicament is characterized by comprising the following steps of:

(1) The adult red-neck longicorn is subjected to trapping in the eclosion period;

(2) After the adult red neck longicorn is trapped, leaching and spraying are sequentially carried out on the spawning positions of the red neck longicorn by adopting 2000 times of liquid of 5.7% emamectin benzoate microcapsules, 2000 times of liquid of 8% cypermethrin microcapsules and 1500 times of liquid of 3% thiacloprid microcapsules at intervals.

2. The method for preventing and controlling red-neck longicorn by using a slow-release medicament leaching spray boom according to claim 1, wherein in the step (1), the method for trapping red-neck longicorn adults is as follows: the sugar-vinegar liquid trapper is hung on the crown of the peach tree, 1-2 sugar-vinegar liquid trappers are hung per mu, and sugar-vinegar liquid is replaced once every 15-20 days.

3. The method for preventing and controlling red neck longicorn by using the slow-release medicament leaching spray boom according to claim 2, wherein the sweet and sour liquid is prepared by mixing sugar, vinegar, wine and water according to a mass ratio of 1:1.5:0.5:18.

4. The method for controlling red neck longicorn by slowly releasing medicament leaching spray bars according to claim 2, wherein the hanging height of the sweet and sour liquid trap is 1.2-1.8 m.

5. The method for controlling red neck longicorn by using a slow release medicament leaching spray boom according to claim 1, wherein in the step (2), the spraying time of the emamectin benzoate microcapsule is 7-10 days after the trapped red neck longicorn adults.

6. The method for controlling red neck longicorn by using a slow release medicament leaching spray boom according to claim 1, wherein in the step (2), the spraying interval of the cypermethrin microcapsule and the emamectin benzoate microcapsule is 15-20 days.

7. The method for controlling red neck longicorn by using a slow release medicament leaching spray boom according to claim 1, wherein in the step (2), the spraying interval of thiacloprid microcapsules and cypermethrin microcapsules is 15-20 days.

8. The method for controlling red neck longicorn by using a slow release medicament leaching spray rod as claimed in claim 1, wherein in the step (2), the leaching spray method comprises the following steps: the preparation is uniformly sprayed on the trunk and the main branch of the trunk, and the inside of the bark gaps at the trunk and the main branch are uniformly wetted with the preparation.