CN116158448A - Application of marine microalgae in improving plant stress resistance and seaweed fertilizer - Google Patents
Application of marine microalgae in improving plant stress resistance and seaweed fertilizer Download PDFInfo
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- CN116158448A CN116158448A CN202310169076.6A CN202310169076A CN116158448A CN 116158448 A CN116158448 A CN 116158448A CN 202310169076 A CN202310169076 A CN 202310169076A CN 116158448 A CN116158448 A CN 116158448A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/03—Algae
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/15—Leaf crops, e.g. lettuce or spinach
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P21/00—Plant growth regulators
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
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- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
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Abstract
The invention discloses application of marine microalgae in improving stress resistance of plants and a seaweed fertilizer. According to the invention, the dunaliella salina is applied to the planting environment where plants are located, so that the stress resistance of the plants is improved, and the growth of the plants is promoted.
Description
Technical Field
The invention relates to the field of marine microalgae, in particular to application of marine microalgae in improving plant stress resistance and a seaweed fertilizer.
Background
Plants often experience adverse conditions during growth, such as high temperature, drought, salt and alkali. Long term stress can cause reduced yields or even death of plants such as crops, causing damage to agricultural production. The saline-alkali soil has insufficient organic matters and low diversity of indigenous microorganisms, and the artificial exogenous microorganisms are easy to lose. Along with the increase of the saline-alkali degree in the soil, the water potential of the soil is reduced, the water absorption of plants is difficult, and the physiological drought of the plants is caused to prevent the plants from growing and developing. In addition, saline-alkali stress can interfere or inhibit normal physiological activities of plant cells, change the structure and function of antioxidant enzyme, break the balance (generation and elimination) of ROS (Reactive oxygen species, active oxygen) in plants, cause oxidation and antioxidant stress, accumulate a large amount of active oxygen, and cause oxidative damage. The traditional saline-alkali soil improvement measures mainly comprise water conservancy and chemical improvement, wherein the water conservancy improvement needs to establish a larger drainage and irrigation system, and the cost is high; although the chemical method takes effect quickly, secondary pollution is easy to cause.
In recent years, the introduction of biological methods has infused new vitality for improvement of saline-alkali soil, such as application of algae resources in agricultural production. However, marine microalgae such as Dunaliella salina have not been reported in saline-alkali soil improvement and plant promotion effects in saline-alkali soil.
Disclosure of Invention
In order to solve the problem of plant growth in a saline-alkali environment, the invention provides application of marine microalgae in improving plant stress resistance and a seaweed fertilizer.
The technical problems of the invention are solved by the following technical scheme:
in a first aspect, the present invention provides the use of a marine microalgae for improving stress resistance of a plant, said marine microalgae being dunaliella salina, applied in a planting environment in which the plant is located.
In a second aspect, the invention also provides an alga fertilizer for improving stress resistance of plants, wherein the alga fertilizer contains salt-containing dunaliella.
Compared with the prior art, the invention has the beneficial effects that:
the application of the marine microalgae in improving the stress resistance of plants and the seaweed fertilizer provided by the invention can improve the stress resistance of plants and promote the growth of the plants by applying the dunaliella salina to the planting environment where the plants are located. The invention can provide technical support for crop planting, especially for planting of rape in saline-alkali environment.
Other advantages of embodiments of the present invention are further described below.
Drawings
FIG. 1 is a graph showing the germination rate of canola seeds versus days in the saline-alkali solution environment experiment of example 1 of the present invention;
FIG. 2 is a graph showing the emergence rate of rape seeds versus days in the saline-alkali liquid environment experiment in example 1 of the present invention;
FIG. 3 is a schematic diagram showing germination or emergence of canola seeds on the seventh day in the saline-alkali liquid environment experiment of example 1 of the present invention;
FIG. 4 is a graph showing the seedling emergence rate of the canola seeds versus days in the saline-alkali soil environment experiment of example 1 of the present invention;
FIG. 5a is a schematic representation of malondialdehyde content in canola plants after 28 days of saline-alkali soil cultivation in example 2 of the present invention;
FIG. 5b is a graph showing the proline content in canola plant after 28 days of saline-alkali soil cultivation in example 2 of this invention;
FIG. 5c is a graph showing the superoxide dismutase content of canola plants after 28 days of saline-alkali soil cultivation in example 2 of this invention;
FIG. 5d is a schematic representation of peroxidase content in canola plants after 28 days of saline-alkali soil cultivation in example 2 of the present invention.
Detailed Description
The invention will be further described with reference to the following drawings in conjunction with the preferred embodiments. It should be noted that, in the case of no conflict, the embodiments and features in the embodiments may be combined with each other.
The embodiment of the invention provides application of marine microalgae in improving stress resistance of plants, wherein the marine microalgae are dunaliella salina, and are applied to planting environments where plants are located.
In some embodimentsIn an example, the planting environment is saline-alkali solution, the dunaliella salina is applied in the saline-alkali solution in the form of at least one of active algae suspension, dry algae powder and dry algae powder algae suspension, wherein each liter of the saline-alkali solution contains 10 percent 8 ～10 9 Dunaliella salina cells.
In some embodiments, the planting environment is saline-alkali soil, the dunaliella salina is applied in the saline-alkali soil in the form of an active algae suspension and/or a dry algae powder algae suspension, wherein each gram of the saline-alkali soil contains 10 percent 6 ～10 7 Dunaliella salina cells.
In some embodiments, the plant is canola.
In some embodiments, the stress resistance refers to resistance to salt alkalinity.
In some embodiments, the increasing stress resistance of the plant is achieved by activating an antioxidant system within the plant.
In some embodiments, the method of activating an antioxidant system in a plant is to increase the activity of an antioxidant enzyme in the plant, the antioxidant enzyme being at least one of peroxidase, superoxide dismutase.
In some embodiments, the active algae suspension is prepared by inoculating dunaliella salina into artificial seawater with the salinity of 35 per mill, culturing for 20-30 days, centrifuging at 4000-6000 r, washing with purified water for 3-4 times, and adding a predetermined amount of purified water; wherein the salinity of the artificial seawater is provided by coral sea salt. Every liter of active algae suspension contains 10 10 ～10 11 Dunaliella salina cells.
In some embodiments, the dry algae powder is prepared by inoculating Dunaliella salina into artificial seawater with the salinity of 35 per mill, culturing for 20-30 days, centrifuging at 4000-6000 r, washing with purified water for 3-4 times, and drying in a baking oven at 30-40 ℃; wherein the salinity of the artificial seawater is provided by coral sea salt.
In some embodiments, the dry algae powder algae suspension is prepared by suspending the dry algae powder in purified water. Every liter of the dry algae powder algae suspension contains 10 percent 9 ～10 10 Radix et rhizoma Du SuccinaeAlgae cells.
In some embodiments, the dunaliella salina is cultured in f/2-Si medium under the following conditions: the illumination intensity is 4000+/-500 lux, and the ratio of the illumination period to the light-dark period is 14h: and 10h.
In some embodiments, the f/2-Si medium comprises 8.82×10 -4 NaNO of M 3 、3.62×10 -5 NaH of M 2 PO 4 ·H 2 O, trace metal element mother liquor and vitamin mother liquor, wherein the trace metal element mother liquor comprises 1.17 multiplied by 10 -5 FeCl of M 3 ·6H 2 O、1.17×10 -5 Na of M 2 EDTA·2H 2 O、3.93×10 -8 CuSO of M 4 ·5H 2 O、2.60×10 -8 Na of M 2 MoO 4 ·2H 2 O、7.65×10 -8 ZnSO of M 4 ·7H 2 O、4.20×10 -8 M CoCl 2 ·6H 2 O and 9.10X10 -7 MnCl of M 2 ·4H 2 O, the vitamin mother liquor comprises 2.96×10 -7 Vitamin B of M 1 、2.05×10 -9 Vitamin H of M and 3.69×10 -10 Vitamin B of M 12 。
The embodiment of the invention also provides a seaweed fertilizer for improving the stress resistance of plants, wherein the seaweed fertilizer contains the salt-containing dunaliella.
In some embodiments, the dunaliella salina is present in the algae fertilizer in the form of at least one of an active algae suspension, a dry algae powder, and a dry algae powder algae suspension.
The invention is further described below by means of specific examples.
(1) Culture of Dunaliella salina:
the present invention was carried out using Dunaliella salina (Dunaliella sp.) strain purchased from Xiamen university.
Before the experiment, the dunaliella salina is firstly domesticated and expanded in an f/2-Si culture medium so as to adapt to the culture conditions of a laboratory. The growth period of the dunaliella salina is about 12 days, and after the algae cells enter the stabilization period, the algae liquid with the volume ratio of 1/10 is taken for inoculation again, so that semi-continuous culture is realized.
The seawater used in the f/2-Si culture medium is artificial seawater (35 g coral sea salt is added into 1L purified water, and the same shall apply below), and the seawater is sterilized in a sterilizing pot under high pressure with the salinity of 35 per mill. The dunaliella salina is cultivated in a phytotron, the illumination intensity is 4000lux, and the ratio of the illumination period to the light-dark period is 14h: and 10h.
In the experiment, 1L of algae solution of dunaliella salina is cultivated in a 3L conical flask, the position of the culture flask is randomly changed for 2 times every day, and the culture flask is rocked, so that the illumination uniformity of the algae solution in the conical flask is ensured.
In the examples of the present invention, F/2-Si medium without adding silicon (i.e., silicon-reduced or-Si) was used to culture Dunaliella salina, and the formulation of the F/2-Si medium is shown in Table 1, wherein "- - -" indicates blank. 950mL of artificial seawater was prepared, added to 950mL of seawater according to the composition and volume shown in Table 1, and then the volume was fixed to 1L with the artificial seawater.
TABLE 1
Preparing trace metal element mother solution: 950mL of ultrapure water was prepared, the volume was set to 1L after adding the trace metal element mother liquor formulation components of Table 2 to the ultrapure water, and the mixture was sterilized by secondary filtration and stored in a refrigerator at 4℃for use, wherein "- - -" in the table indicates blank.
TABLE 2
Preparing a vitamin mother solution: 950mL of ultrapure water was prepared, the volume was set to 1L after adding the vitamin mother liquor formulation components according to Table 3 to the ultrapure water, and the mixture was frozen in a refrigerator at-20℃for use after secondary filtration sterilization, wherein "- - -" indicates blank.
TABLE 3 Table 3
(2) Preparation of active algae suspension:
inoculating the dunaliella salina obtained by culturing in the step (1) into 1L of artificial seawater, centrifuging 4000-6000 r after culturing for 30 days, washing 3-4 times with purified water, adding purified water, shaking uniformly, and preparing active algae suspension, wherein the algae cell density is as follows: 6.88*10 10 cells/L (6.88×10 per liter of active algae suspension) 10 Dunaliella salina cells).
(3) Preparing dry algae powder:
inoculating the dunaliella salina obtained by culturing in the step (1) into 1L of artificial seawater, culturing for 30 days, centrifuging at 4000-6000 r, washing with purified water for 3-4 times, and drying in a baking oven at 30-40 ℃ for 48 hours to prepare dry algae powder.
(4) Preparing a dry algae powder algae suspension:
suspending the dry algae powder obtained in the step (3) in purified water to prepare a dry algae powder algae suspension, wherein the algae cell density is as follows: 6.88*10 10 cells/L (6.88×10 per liter of active algae suspension) 10 Dunaliella salina cells).
The following takes plants as rape as an example, and the experimental process and detection parameters are as follows: experimental example 1: the dunaliella salina improves the germination experiment of the small rape seeds in the saline-alkali environment.
1. Material preparation:
the experimental object: rape seed.
Drug configuration:
(1) Salt alkali liquor: purified water was used to prepare 20mmol of a salt lye (NaCl: na) 2 SO 4 ：NaHCO 3 ：Na 2 CO 3 =1: 9:9:1 (molar ratio)).
(2) Saline-alkali soil: 225g of simulated matrix (nutrient soil and vermiculite in a volume ratio of 1:1) was filled with saline solution three times (interval: the next time after the saline solution to be filled has completely penetrated), 100ml each time.
(3) Common soil: 225g of simulated matrix (nutrient soil and vermiculite in a volume ratio of 1:1) was poured three times with purified water (interval: the next time after the saline solution to be poured had completely penetrated), 100ml each time.
2. The experimental process comprises the following steps:
after the canola seeds were sterilized with 20% by volume hydrogen peroxide for 5 minutes, they were washed with purified water 5 times to wash off the hydrogen peroxide solution, and the following experiments were performed:
(1) Saline-alkali solution environmental experiment:
active algae suspension-saline-alkali liquor (named TA group): adding the active algae suspension into 500ml of saline alkali solution to enable the suspension to contain 6.88 x 10 per liter of saline alkali solution 8 Obtaining active algae suspension-saline solution by using dunaliella salina cells. Placing gauze in the germination device, adding 500ml of active algae suspension-saline-alkali solution, selecting 10 small rape seeds with the same size, placing in the germination device, and water culturing for 7 days.
Dry algae powder-saline-alkali liquor (named BA group): adding dry algae powder into 500ml of saline alkali solution, wherein each liter of saline alkali solution contains 6.88 x 10 8 The Dunaliella salina cells are salted to obtain dry algae powder-brine solution. Placing gauze in the germination device, adding 500ml of dry algae powder-saline-alkali solution, selecting 10 small rape seeds with the same size, placing in the germination device, and water culturing for 7 days.
Saline (designated CKY group): placing gauze in the germination device, adding 500ml of saline alkali solution, selecting 10 rape seeds with the same size, placing in the germination device, and water culturing for 7 days.
Purified water (named CK group): placing gauze in the germination device, adding 500ml purified water, selecting 10 small rape seeds with the same size, placing in the germination device, and water culturing for 7 days.
The above groups were all equal in volume, while each group was set with 3 parallel experiments.
Measuring the index:
the seed germination rate and emergence rate were recorded daily, wherein the canola seed germination rate-days are shown in fig. 1, and the canola seed emergence rate-days are shown in fig. 2 and table 4, with the data being the average.
TABLE 4 Table 4
The schematic diagram of germination or emergence of the rape seeds of three groups of parallel experiments of CK group, CKY group, BA group and TA group in the saline-alkali liquid environment experiment on the 7 th day is shown in figure 3.
From the data in fig. 1, 2, 3 and table 4, it can be seen that the germination of the canola seeds was inhibited in the simulated saline-alkali solution environment, but the germination and emergence of the canola seeds was promoted after the addition of the active algae suspension and the dry algae powder.
(2) Salt-alkali soil environment experiment:
active algae suspension-saline-alkali soil (designated as TA group): selecting 10 rape seeds with the same size from saline-alkali soil, placing in a pot, uniformly applying 100ml of active algae suspension diluted 20 times so that the average content of the active algae suspension per gram of soil is 1.53 x 10 6 The Dunaliella salina cells were cultured for 7 days.
Dry algae powder algae suspension-saline-alkali soil (named BA group): selecting 10 rape seeds with the same size from saline-alkali soil, placing in a pot, uniformly applying 100ml of dry algae powder algae suspension diluted 20 times, so that the average content of the algae powder suspension per gram of soil is 1.53 x 10 6 The Dunaliella salina cells were cultured for 7 days.
Saline-alkali soil (designated CKY group): in saline-alkali soil, 10 rape seeds with the same size are selected and placed in a pot, 100mL of purified water is uniformly applied, and the rape seeds are cultured for 7 days.
Common soil (named CK group): in common soil, 10 rape seeds with the same size are selected and placed in a pot, 100mL of purified water is uniformly applied, and the rape seeds are cultured for 7 days.
The above groups were all equal in volume, while each group was set with 3 parallel experiments.
Measuring the index:
seed emergence rate was recorded daily. The seedling emergence rate-days of the rape seeds in the saline-alkali soil environment experiment are shown in fig. 4 and table 5, wherein the data are all average numbers.
TABLE 5
From the data in fig. 4 and table 5, it can be seen that the emergence of the canola seeds is inhibited in the saline-alkali soil environment, and the emergence rate of the canola seeds can be significantly promoted by adding the active algae suspension and the dry algae powder algae suspension.
Experimental example 2: the stress resistance experiment of the rape under the saline-alkali soil environment is improved by the dunaliella salina.
1. Material preparation:
the experimental object: rape seed. Sterilizing rape seed with 20% hydrogen peroxide for 5min, washing with purified water for 5 times, and washing off hydrogen peroxide solution.
Drug configuration:
(1) Salt alkali liquor: purified water was used to prepare 20mmol of a salt lye (NaCl: na) 2 SO 4 ：NaHCO 3 ：Na 2 CO 3 =1: 9:9:1 (molar ratio)).
(2) Saline-alkali soil: 225g of simulated matrix (nutrient soil and vermiculite in a volume ratio of 1:1) was filled with saline solution three times (interval: the next time after the saline solution to be filled has completely penetrated), 100ml each time.
(3) Common soil: 225g of simulation matrix (nutrient soil and vermiculite in a volume ratio of 1:1), purified water is poured three times (interval: the next time after the saline solution to be poured is completely permeated), 100ml each time
2. Experimental procedure
Saline-alkali soil environment:
active algae suspension-saline-alkali soil (designated as TA group): selecting 10 rape seeds with the same size from saline-alkali soil, placing in a pot, uniformly applying 100ml of active algae suspension diluted 20 times, wherein the average content of the active algae suspension per gram of soil is 1.53 x 10 6 The Dunaliella salina cells were cultured for 28 days.
Dry algaePowder algae suspension-saline-alkali soil (designated as TA group): selecting 10 rape seeds with the same size from saline-alkali soil, placing in a pot, uniformly applying 100ml of dry algae powder algae suspension diluted 20 times, wherein the average content of the dry algae powder suspension per gram of soil is 1.53 x 10 6 The Dunaliella salina cells were cultured for 28 days.
Saline-alkali soil (designated CKY group): in saline-alkali soil, 10 rape seeds with the same size are selected and placed in a pot, 100ml of purified water is uniformly applied, and the rape seeds are cultured for 28 days.
Common soil (named CK group): in common soil, 10 rape seeds with the same size are selected and placed in a pot, 100ml of purified water is uniformly applied, and the rape seeds are cultured for 28 days.
The above groups were all equal in volume, while each group was set with 3 parallel experiments.
3. Measuring the index:
after 28 days of culture in saline-alkali soil environment, measurement was made of Malondialdehyde (MDA), proline (Pro), superoxide dismutase (SOD), peroxidase (POD) of canola. Wherein the Malondialdehyde (MDA) content of each group of canola is shown in FIG. 5a, the proline (Pro) content of each group of canola is shown in FIG. 5b, the superoxide dismutase (SOD) content of each group of canola is shown in FIG. 5c, and the Peroxidase (POD) content of each group of canola is shown in FIG. 5 d. The Malondialdehyde (MDA), proline (Pro), superoxide dismutase (SOD), and Peroxidase (POD) contents of the respective groups of canola are shown in Table 6, and the data are all average numbers.
TABLE 6
From the data in FIGS. 5a-5d and Table 6, it can be seen that the proline (Pro) content of canola, to which active algae suspension (TA group) and dry algae powder algae suspension (BA group) are applied, is reduced and plant saline-alkali stress is reduced in saline-alkali soil environment. Meanwhile, the content of Malondialdehyde (MDA) of the canola is reduced, the peroxidation degree of the membrane lipid is reduced, and the oxidative stress degree is reduced as a whole. The application of the active algae suspension and the dry algae powder algae suspension can improve the content of plant protection enzymes (POD, SOD) and is beneficial to the oxidation stress resistance of the rape.
The embodiment of the invention also provides a seaweed fertilizer for improving the stress resistance of plants, wherein the seaweed fertilizer contains the salt-containing dunaliella. In the specific implementation, the dunaliella salina exists in the seaweed fertilizer in the form of active seaweed suspension, dry seaweed powder or dry seaweed powder seaweed suspension.
The embodiment of the invention has the beneficial effects that: the embodiment of the invention researches the effect of the dunaliella salina on improving the stress resistance of plants and the growth promotion effect on the plants, and the dunaliella salina can be used as a potential seaweed fertilizer, can improve the stress resistance of the plants in a saline-alkali environment and promote the growth of the plants. Both different production types (active algae suspension and dry algae powder) of the dunaliella salina can improve the seed germination rate, the emergence speed and the stress resistance of the rape. However, compared with active algae suspension, the dry algae powder has relatively high cost due to the extra operation step of 'drying'. The research of the invention provides technical support for crop planting, especially for planting of rape in saline-alkali environment.
The foregoing is a further detailed description of the invention in connection with the preferred embodiments, and it is not intended that the invention be limited to the specific embodiments described. It will be apparent to those skilled in the art that several equivalent substitutions and obvious modifications can be made without departing from the spirit of the invention, and the same should be considered to be within the scope of the invention.
Claims (10)
1. The application of marine microalgae in improving plant stress resistance is characterized in that the marine microalgae are dunaliella salina which are applied in a planting environment where plants are located.
2. The use according to claim 1, wherein the planting environment is saline-alkali liquor, the dunaliella salina is applied in the saline-alkali liquor in the form of at least one of an active algae suspension, a dry algae powder and a dry algae powder algae suspension, wherein the saline-alkali liquor contains 10 per liter of saline-alkali liquor 8 ～10 9 Dunaliella salina cells.
3. The use according to claim 1, wherein the planting environment is saline-alkali soil, the dunaliella salina being applied in the saline-alkali soil in the form of an active algae suspension and/or a dry algae powder algae suspension, wherein 10 per gram of the saline-alkali soil 6 ～10 7 Dunaliella salina cells.
4. The use according to claim 1, wherein the plant is canola.
5. The use according to claim 1, wherein the stress resistance is saline-alkali resistance; preferably, the improvement of plant stress tolerance is achieved by activating an antioxidant system in the plant; further preferably, the method of activating an antioxidant system in a plant is to increase the activity of an antioxidant enzyme in the plant, wherein the antioxidant enzyme is at least one of peroxidase and superoxide dismutase.
6. A use according to claim 2 or 3, wherein:
the active algae suspension is prepared by inoculating dunaliella salina into artificial seawater with the salinity of 35 per mill, culturing for 20-30 days, centrifuging at 4000-6000 r, washing with purified water for 3-4 times, and adding a predetermined amount of purified water;
the dry algae powder is prepared by inoculating dunaliella salina into artificial seawater with the salinity of 35 per mill, culturing for 20-30 days, centrifuging at 4000-6000 r, washing with purified water for 3-4 times, and drying in a baking oven at 30-40 ℃;
the dry algae powder algae suspension is prepared by suspending the dry algae powder in purified water;
wherein the salinity of the artificial seawater is provided by coral sea salt.
7. A use according to claim 2 or 3, wherein: the dunaliella salina is obtained by culturing in an f/2-Si culture medium, and the culture conditions are as follows: the illumination intensity is 4000+/-500 lux, and the ratio of the illumination period to the light-dark period is 14h: and 10h.
8. The use according to claim 7, wherein: the f/2-Si medium comprises 8.82×10 -4 NaNO of M 3 、3.62×10 -5 NaH of M 2 PO 4 ·H 2 O, trace metal element mother liquor and vitamin mother liquor, wherein the trace metal element mother liquor comprises 1.17 multiplied by 10 -5 FeCl of M 3 ·6H 2 O、1.17×10 -5 Na of M 2 EDTA·2H 2 O、3.93×10 -8 CuSO of M 4 ·5H 2 O、2.60×10 -8 Na of M 2 MoO 4 ·2H 2 O、7.65×10 -8 ZnSO of M 4 ·7H 2 O、4.20×10 -8 M CoCl 2 ·6H 2 O and 9.10X10 -7 MnCl of M 2 ·4H 2 O, the vitamin mother liquor comprises 2.96×10 -7 Vitamin B of M 1 、2.05×10 -9 Vitamin H of M and 3.69×10 -10 Vitamin B of M 12 。
9. An alga fertilizer for improving stress resistance of plants, which is characterized in that: the seaweed fertilizer contains salted Dunaliella salina.
10. The seaweed fertilizer of claim 9, wherein: the dunaliella salina is present in the algae fertilizer in the form of at least one of an active algae suspension, a dry algae powder, and a dry algae powder algae suspension.
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