CN116144486B - Thallus filtering diluter and use method - Google Patents
Thallus filtering diluter and use method Download PDFInfo
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- CN116144486B CN116144486B CN202310157580.4A CN202310157580A CN116144486B CN 116144486 B CN116144486 B CN 116144486B CN 202310157580 A CN202310157580 A CN 202310157580A CN 116144486 B CN116144486 B CN 116144486B
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- 238000000034 method Methods 0.000 title claims abstract description 19
- 238000001914 filtration Methods 0.000 title claims abstract description 18
- 239000012452 mother liquor Substances 0.000 claims abstract description 95
- 238000010790 dilution Methods 0.000 claims abstract description 69
- 239000012895 dilution Substances 0.000 claims abstract description 69
- 239000007788 liquid Substances 0.000 claims abstract description 37
- 238000007789 sealing Methods 0.000 claims abstract description 33
- 239000003085 diluting agent Substances 0.000 claims abstract description 28
- 238000003860 storage Methods 0.000 claims abstract description 17
- 238000007865 diluting Methods 0.000 claims abstract description 11
- 230000008569 process Effects 0.000 claims abstract description 11
- 241001515965 unidentified phage Species 0.000 claims abstract description 10
- 239000010413 mother solution Substances 0.000 claims abstract description 3
- 230000000903 blocking effect Effects 0.000 claims description 37
- 238000005303 weighing Methods 0.000 claims description 13
- 238000005192 partition Methods 0.000 claims description 8
- 230000001580 bacterial effect Effects 0.000 claims description 7
- 230000008859 change Effects 0.000 claims description 6
- 238000007599 discharging Methods 0.000 claims description 6
- 239000012535 impurity Substances 0.000 claims description 6
- 230000000149 penetrating effect Effects 0.000 claims description 6
- 239000011521 glass Substances 0.000 claims description 3
- 238000005086 pumping Methods 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 4
- 238000002156 mixing Methods 0.000 abstract description 3
- 238000003756 stirring Methods 0.000 abstract description 3
- 238000003113 dilution method Methods 0.000 abstract description 2
- 239000000243 solution Substances 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 241000700605 Viruses Species 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000013585 weight reducing agent Substances 0.000 description 1
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- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/34—Internal compartments or partitions
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- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/18—Flow directing inserts
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- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
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- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
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Abstract
The invention provides a thallus filtering diluter and a use method thereof, which relate to the technical field of phage filtering dilution and are used for solving the problems that mother liquor weight is reduced in the filtering process after transferring, dilution is changed, and the concentration is too high due to dilution according to the original proportion, and meanwhile, natural mixing is needed in the dilution process, external stirring is difficult to adapt, and the dilution concentration is not uniform easily occurs at the moment, and the thallus filtering diluter comprises the following components: a diluter; the front end of the diluter is hinged with a lower sealing door and an upper sealing door, and the lower sealing door is positioned below the upper sealing door; the lower end of the side wall of the rear end of the diluter is fixedly provided with a diluting liquid storage box; a metering pump is fixedly arranged in the middle of the upper end of the diluent storage box; and a control panel is fixedly arranged at the upper end of the side wall of the back of the diluter. The invention achieves the purpose of accurate dilution, ensures the accuracy of the dilution concentration of the bacteriophage mother solution, and realizes the effect of accurate control of the dilution concentration.
Description
Technical Field
The invention relates to the technical field of phage filtration dilution, in particular to a bacterial body filtration diluter and a using method thereof.
Background
Along with the continuous improvement of economic level, the cultivation technology of phage has obtained very big development, the phage is the virus of invading bacteria, also is the genetic material that gives host bacterium biological property, it usually exists in places such as earth, animal intestinal, need to filter it to dilute to the phage in the process of cultivating, this just needs to use to filter diluting device, and in the process of phage dilution, usually adopt to weigh mother liquor in advance, then shift to dilute filtration treatment, and can cause mother liquor weight reduction in filtration process after shifting, lead to dilution change, and dilute according to original ratio and can cause the too high problem of concentration, and simultaneously need natural mixing in diluting process, be difficult to adapt to outside stirring, easily appear the inhomogeneous problem of dilution concentration this moment.
Disclosure of Invention
In view of the above, the present invention provides a bacterial filtering diluter and a use method thereof, so as to solve the problems that the weight of mother solution is reduced in the filtering process after transferring, the dilution is changed, and the concentration is too high due to dilution according to the original proportion, and meanwhile, natural mixing is required in the dilution process, so that the bacterial filtering diluter is difficult to adapt to external stirring, and the problem of uneven dilution concentration is easy to occur at the moment.
The invention provides a thallus filtering diluter and a use method thereof, which specifically comprise the following steps: a diluter; the front end of the diluter is hinged with a lower sealing door and an upper sealing door, and the lower sealing door is positioned below the upper sealing door; the lower end of the side wall of the rear end of the diluter is fixedly provided with a diluting liquid storage box; a metering pump is fixedly arranged in the middle of the upper end of the diluent storage box; a control panel is fixedly arranged at the upper end of the side wall of the back of the diluter; the upper end of the inner cavity of the diluter is longitudinally and slidably provided with a mother liquor box, and the lower end of the inner cavity of the diluter is longitudinally and slidably provided with a dilution box; a temperature control lamp is fixedly arranged on the side wall of the diluter at the position of the dilution box and is electrically connected with a controller in a control panel; a control blocking piece is vertically arranged in the middle of the mother liquor box; the weighing platform is fixedly installed at the rear part of the bottom of the inner cavity of the diluter in an embedded manner.
Optionally, guide grooves are longitudinally formed in the positions, corresponding to the mounting positions of the mother liquor box, of the side walls at the left end and the right end of the inner cavity of the diluter; guide surplus grooves are longitudinally formed in the side walls of the left end and the right end of the inner cavity of the diluter, and the bottoms of the guide surplus grooves are overlapped with the bottom plate of the inner cavity of the diluter; an upper sealing plate is fixedly arranged at the rear part of the inner cavity of the diluter above the position of the mother liquor box, and the bottom of the upper sealing plate is in sliding tangency with the upper end of the mother liquor box; a middle partition plate is fixedly arranged at the front part of the inner cavity of the diluter below the mother liquor box, and a middle division hole is longitudinally formed in the middle of the middle partition plate; a stop slot is formed in the middle of the bottom plane of the inner cavity of the diluter.
Optionally, a transparent glass liquid level window is embedded in the back side wall of the diluent storage box.
Optionally, the metering pump draft tube extends into the diluent storage tank interior cavity; the output of measuring pump is connected with the delivery main pipe, and the delivery main pipe is connected with two delivery branch pipes, and the delivery main pipe is installed the automatically controlled valve with delivery branch pipe junction, and two delivery branch pipes are worn to be in the dilution case top through the diluter left and right sides respectively, and two delivery branch pipes are located the dilution case top and are left and right sides looks staggered distribution, and the end of delivery branch pipe is circular-arc structure, and the end of two delivery branch pipes is relative state about, and the circular arc part of delivery branch pipe is the state of slope upset downwards.
Optionally, guide fins A are fixedly arranged on the side walls of the left end and the right end of the mother liquor box respectively, and the guide fins A are slidably arranged in the guide grooves; a rectangular lower liquid tank is arranged in the middle of the mother liquid tank, and a rectangular central hole penetrating up and down is arranged in the middle of the bottom of the lower liquid tank; a round liquid outlet hole is vertically formed in the bottom of the liquid outlet groove around the central hole.
Optionally, longitudinal guide fins B are fixedly installed on the side walls of the left end and the right end of the dilution box respectively, and the upper end faces of the guide fins B are tangent to the upper groove walls of the guide surplus grooves; a disc-shaped magnetic supporting tray is vertically and fixedly arranged in the middle of the dilution box upwards, and an arc-shaped diversion wing is arranged on the annular part on the upper end surface of the magnetic supporting tray; a pulling plate is fixedly arranged in the middle of the side wall of the front end of the dilution box; the middle part of arm-tie perpendicular slidable mounting has the backstop round pin post, and when diluting case was built-in, the lower extreme of backstop round pin post fell into the backstop slotted hole.
Optionally, the control blocking piece is of a rectangular structure, the control blocking piece is consistent with the structure of the lower liquid tank, when the control blocking piece is embedded in the lower liquid tank, the upper end face of the control blocking piece is flush with the bottom plane of the inner cavity of the mother liquor tank, the middle part of the bottom plane of the control blocking piece is a rectangular central column vertically downward, and the central column is inserted into the central hole in a penetrating manner; the lower end of the central column is fixedly connected with a disc-shaped magnetic collet filter disc, the upper end of the magnetic collet filter disc is of an inwards concave cambered surface structure, and the magnetic collet filter disc is made of a magnet with the same polarity as the magnetic collet filter disc; and a stop ring is fixedly arranged on the central column below the mother liquor box, the control blocking piece is provided with blocking heads corresponding to the lower liquid holes on the bottom plane, and when the control blocking piece moves upwards, the stop ring is contacted with the bottom of the mother liquor box, and the blocking heads are separated from the lower liquid holes.
Optionally, the weighing platform detects stable weight signals and then transmits the stable weight signals to a controller in a control panel, and the controller transmits the stable weight signals to a metering pump and an electric control valve to convey diluent in corresponding proportion to a diluent box.
Firstly, opening an upper sealing door, pulling out a mother liquor box, injecting bacteriophage mother liquor into the mother liquor box, and adding bacteriophage mother liquor into the mother liquor box;
secondly, the mother liquor box is pushed inwards to reset, because the polarities of the magnetic support tray and the magnetic support tray are consistent, after alignment, the magnetic support tray is jacked up, the blocking piece is controlled to ascend, phage mother liquor flows onto the magnetic support tray from the liquid outlet holes and overflows onto the magnetic support tray from the edges of the magnetic support tray, in the process, impurities in the phage mother liquor stay in the concave parts of the inner grooves of the magnetic support tray, and the flowing phage mother liquor is uniformly distributed in the dilution box in a rotary scattering state after being guided by the guide fins;
finally, the weighing platform detects a weight change signal until the weight is stable, the weight value is transmitted to the controller for processing, the controller transmits the diluent with corresponding proportion to the metering pump and the electric control valve to the diluent box for diluting the bacteriophage mother liquor, after the bacteriophage mother liquor is cultured, the lower sealing door is opened, and the diluent box is pulled out for transferring and storing after the bacteriophage mother liquor is diluted.
Advantageous effects
1. According to the invention, after phage mother liquor is added into the mother liquor box, the mother liquor box is pushed and reset, as the polarities of the magnetic support tray and the magnetic support tray are consistent, after alignment, the magnetic support tray is jacked up, the blocking piece is controlled to rise, phage mother liquor flows onto the magnetic support tray from the lower liquor hole and overflows onto the magnetic support tray from the edge of the magnetic support tray, in the process, impurities in the phage mother liquor stay in the low concave part of the inner groove of the magnetic support tray, the flowing phage mother liquor is uniformly distributed in the dilution box in a rotating scattering state after being guided by the guide fins, the weighing table detects a weight change signal at the moment until the weight is stable, the weight value is transmitted to the controller for processing, and the controller is transmitted to the metering pump and the electric control valve for transmitting dilution liquid in corresponding proportion to the dilution box for phage mother liquor dilution, so that the purpose of accurate dilution concentration of phage mother liquor is achieved, the accuracy of dilution concentration of phage mother liquor is ensured, and the effect of accurate dilution concentration control is realized.
2. According to the invention, the left conveying branch pipe and the right conveying branch pipe are positioned above the dilution box and are distributed in a left-right staggered manner, the tail ends of the conveying branch pipes are of arc-shaped structures, the tail ends of the left conveying branch pipe and the right conveying branch pipe are in opposite states, and the arc parts of the conveying branch pipes are in inclined downward overturning states.
Drawings
In order to more clearly illustrate the technical solution of the embodiments of the present invention, the drawings of the embodiments will be briefly described below.
The drawings described below are only for illustration of some embodiments of the invention and are not intended to limit the invention.
In the drawings:
fig. 1 is a schematic view of an upper right front perspective structure of an embodiment of the present invention.
Fig. 2 is a schematic view of an upper left rear perspective view of an embodiment of the present invention.
Fig. 3 is a schematic view of the lower right front perspective structure of an embodiment of the present invention.
Fig. 4 is an isometric view of an open state of a lower and upper closure in accordance with an embodiment of the present invention.
Fig. 5 is an isometric view of a mother liquor tank in an up-shift state according to an embodiment of the present invention.
FIG. 6 is a schematic diagram of an isometric view of a mother liquor tank and control block in a separated state according to an embodiment of the present invention.
FIG. 7 is a schematic view of an underside view of a control seal portion of an embodiment of the present invention.
Fig. 8 is an isometric view of a dilution tank in a removed state according to an embodiment of the invention.
List of reference numerals
1. A dilution tank; 101. a guide groove; 102. guiding the surplus groove; 103. a middle partition plate; 10301. a middle dividing hole; 104. an upper sealing plate; 105. a stop slot; 2. sealing the door; 3. sealing the door; 4. a diluent storage tank; 401. a liquid level window; 5. a metering pump; 501. a conveying main pipe; 502. an electric control valve; 503. a conveying branch pipe; 6. a control panel; 7. a mother liquor tank; 701. a guide fin A; 702. a liquid discharging tank; 70201. a liquid discharging hole; 70202. a central bore; 8. a temperature control lamp; 9. a dilution tank; 901. a guide fin B; 902. a magnetic support tray; 90201. a diversion wing; 903. pulling a plate; 90301. a stop pin; 10. controlling the blocking piece; 1001. a center column; 100101, a stop ring; 1002. a magnetic bottom support filter disc; 1003. a blocking head; 11. weighing table.
Description of the embodiments
In order to make the objects, aspects and advantages of the technical solution of the present invention more clear, the technical solution of the embodiment of the present invention will be clearly and completely described below with reference to the accompanying drawings of the specific embodiment of the present invention.
Examples: please refer to fig. 1 to 8:
the invention provides a bacterial filtering diluter and a use method thereof, comprising a diluter 1; the front end of the diluter 1 is hinged with a lower sealing door 2 and an upper sealing door 3, and the lower sealing door 2 is positioned below the upper sealing door 3; the lower end of the side wall of the rear end of the diluter 1 is fixedly provided with a diluting liquid storage box 4; a metering pump 5 is fixedly arranged in the middle of the upper end of the diluent storage box 4; a control panel 6 is fixedly arranged at the upper end of the side wall of the back of the diluter 1; the upper end of the inner cavity of the diluter 1 is longitudinally and slidably provided with a mother liquor box 7, and the lower end of the inner cavity of the diluter 1 is longitudinally and slidably provided with a dilution box 9; a temperature control lamp 8 is fixedly arranged on the side wall of the diluter 1 where the dilution box 9 is positioned, and the temperature control lamp 8 is electrically connected with a controller in the control panel 6; a control blocking piece 10 is vertically arranged in the middle of the mother liquor tank 7; the weighing table 11 is fixedly installed at the rear part of the bottom of the inner cavity of the diluter 1.
Wherein, the side walls at the left and right ends of the inner cavity of the diluter 1 are longitudinally provided with guide grooves 101 at the positions corresponding to the installation positions of the mother liquor box 7, so that the mother liquor box 7 can longitudinally move in the guide grooves 101, thereby facilitating the addition of bacteriophage mother liquor and the cleaning and maintenance of the mother liquor box 7;
guide surplus grooves 102 are longitudinally formed in the side walls of the left end and the right end of the inner cavity of the diluter 1, corresponding to the dilution box 9, the bottoms of the guide surplus grooves 102 are overlapped with the bottom plate of the inner cavity of the diluter 1, the dilution box 9 can longitudinally move in the guide surplus grooves 102, and the dilution box 9 can downwards move above the weighing table 11 so as to be convenient for bearing;
an upper sealing plate 104 is fixedly arranged at the rear part of the inner cavity of the diluter 1 above the position of the mother liquor box 7, the bottom of the upper sealing plate 104 is in sliding tangency with the upper end of the mother liquor box 7, and the upper sealing plate can be used for sealing the mother liquor box 7 to prevent impurities from entering the sealed mother liquor box 7;
a middle partition plate 103 is fixedly arranged at the position, close to the front, of the inner cavity of the diluter 1 below the mother liquor tank 7, a middle division hole 10301 is longitudinally formed in the middle of the middle partition plate 103, the middle partition plate 103 is used for separating the mother liquor tank 7 and the dilution tank 9, and the middle division hole 10301 can be used for providing a path for the mother liquor tank 7 to move out so as to control the blocking piece 10 to pass through partially;
a pulling plate 903 is fixedly arranged in the middle of the side wall of the front end of the dilution box 9; the middle part of arm-tie 903 is perpendicular slidable mounting has stop pin 90301, and when dilution case 9 is built-in, stop pin 90301's lower extreme falls into the backstop slotted hole 105, and backstop slotted hole 105 has been seted up to the middle part position department of diluter 1 inner chamber bottom plane for cooperate with stop pin 90301, be used for making dilution case 9 place after built-in stable.
Wherein a transparent glass level window 401 is embedded in the back side wall of the diluent storage box 4 for observing the diluent storage amount in the diluent storage box 4.
Wherein the pumping pipe of the metering pump 5 extends into the inner cavity of the diluent storage box 4; the output end of the metering pump 5 is connected with a conveying main pipe 501, the conveying main pipe 501 is connected with two conveying branch pipes 503, an electric control valve 502 is arranged at the joint of the conveying main pipe 501 and the conveying branch pipes 503, the two conveying branch pipes 503 penetrate through the upper part of the dilution box 9 respectively through the left side and the right side of the diluter 1, the left side and the right side of the conveying branch pipes 503 are distributed in a staggered mode, the tail ends of the conveying branch pipes 503 are of arc-shaped structures, the tail ends of the left side and the right side of the conveying branch pipes 503 are in opposite states, the arc-shaped parts of the conveying branch pipes 503 are in inclined downward overturning states, when quantitative diluent is conveyed into the dilution box 9, due to the special structure of the conveying branch pipes 503, the rotary jetting states can be supported after the diluent is output from the conveying branch pipes 503, so that the diluent is quickly mixed with bacteriophage mother liquid in the dilution box 9, and the purpose of quick dilution is achieved.
Guide wings A701 are fixedly arranged on the side walls of the left end and the right end of the mother liquor box 7 respectively, and the guide wings A701 are slidably arranged in the guide groove 101; a rectangular lower liquid tank 702 is arranged in the middle of the mother liquid tank 7, and a rectangular central hole 70202 penetrating up and down is arranged in the middle of the bottom of the lower liquid tank 702; a round liquid discharging hole 70201 is vertically formed in the bottom of the liquid discharging groove 702 around the center hole 70202, longitudinal guide fins B901 are fixedly arranged on the side walls of the left end and the right end of the dilution tank 9 respectively, and the upper end surfaces of the guide fins B901 are tangent to the upper groove wall of the guide surplus groove 102; a disc-shaped magnetic supporting disc 902 is vertically and fixedly arranged in the middle of the dilution tank 9 upwards, an arc-shaped diversion wing 90201 is arranged on the annular part on the upper end surface of the magnetic supporting disc 902, the control blocking piece 10 is of a rectangular structure, the control blocking piece 10 is consistent with the structure of the lower liquid tank 702, when the control blocking piece 10 is embedded in the lower liquid tank 702, the upper end surface of the control blocking piece 10 is flush with the bottom plane of the inner cavity of the mother liquor tank 7, the middle part of the bottom plane of the control blocking piece 10 is a vertically and downwards rectangular central column 1001, and the central column 1001 is inserted into the central hole 70202 in a penetrating manner; the lower end of the center column 1001 is fixedly connected with a disc-shaped magnetic bottom support filter disc 1002, the upper end of the magnetic bottom support filter disc 1002 is of a concave cambered surface structure, and the magnetic bottom support filter disc 1002 is made of a magnet with the same polarity as the magnetic support disc 902; a stop ring 100101 is fixedly arranged on a center column 1001 below a mother liquor box 7, a blocking head 1003 is distributed on the bottom plane of a control blocking piece 10 corresponding to a lower liquor hole 70201, when the control blocking piece 10 moves upwards, the stop ring 100101 is contacted with the bottom of the mother liquor box 7, the blocking head 1003 is separated from the lower liquor hole 70201, a stable weight signal is detected by a weighing table 11 and then is transmitted to a controller in a control panel 6, the controller is transmitted to a metering pump 5 and an electric control valve 502 to convey diluent in a corresponding proportion to a dilution box 9, when phage mother liquor is added into the mother liquor box 7, the mother liquor box 7 is pushed inwards to reset, as the polarities of a magnetic bearing tray 902 and a magnetic bearing tray 1002 are consistent, when the magnetic bearing tray 1002 is jacked up after alignment, the control blocking piece 10 is lifted, phage mother liquor flows from the lower liquid hole 70201 to the magnetic bottom support filter tray 1002 and overflows from the edge of the magnetic bottom support filter tray 1002 to the magnetic bottom support tray 902, in the process, impurities in phage mother liquor stay in the concave parts of the inner grooves of the magnetic bottom support filter tray 1002, the flowing phage mother liquor is uniformly distributed in the dilution box 9 in a rotating scattering state after being guided by the guide fins 90201, the weighing table 11 detects a weight change signal at the moment, after the weight is stable, the weight value is transmitted to the controller for processing, the controller is transmitted to the metering pump 5 and the electric control valve 502 to transmit diluent in corresponding proportion to the dilution box 9 for diluting the phage mother liquor, so that the purpose of accurate dilution is achieved, the accuracy of the dilution concentration of the phage mother liquor is ensured, and the effect of accurately controlling the dilution concentration is achieved.
Specific use and action of the embodiment: in the use process of the invention, the upper sealing door 3 is opened, the mother liquor box 7 is pulled out, phage mother liquor is injected into the mother liquor box 7, after phage mother liquor is added into the mother liquor box 7, the mother liquor box 7 is pushed in to reset, as the polarities of the magnetic bearing tray 902 and the magnetic bearing tray 1002 are consistent, after alignment, the magnetic bearing tray 1002 is jacked up, the sealing piece 10 is controlled to rise, phage mother liquor flows onto the magnetic bearing tray 1002 from the lower liquor hole 70201 and overflows onto the magnetic bearing tray 902 from the edge of the magnetic bearing tray 1002, in the process, impurities in phage mother liquor stay in the concave grooves in the magnetic bearing tray 1002, the flowing phage mother liquor is uniformly distributed in the dilution box 9 in a rotating scattering state after being guided by the guide wings 90201, the weighing platform 11 detects a weight change signal at the moment, after the weight value is stabilized, the weight value is transmitted to the controller to be processed, the controller is transmitted to 5 and the electronic control valve 502 to convey the dilution liquid with the corresponding proportion to the dilution box 9, the phage mother liquor is diluted, after the phage mother liquor is diluted, and after the phage mother liquor is transferred to the dilution box 2 is pulled out, the dilution box is diluted, and the dilution box is preserved.
Claims (3)
1. A bacterial cell filtration diluter, comprising: a diluter (1); the front end of the diluter (1) is hinged with a lower sealing door (2) and an upper sealing door (3), and the lower sealing door (2) is positioned below the upper sealing door (3); the lower end of the side wall of the rear end of the diluter (1) is fixedly provided with a diluting liquid storage box (4); a metering pump (5) is fixedly arranged in the middle of the upper end of the diluent storage box (4); a control panel (6) is fixedly arranged at the upper end of the side wall of the back of the diluter (1); the upper end of the inner cavity of the diluter (1) is longitudinally and slidably provided with a mother liquor box (7), and the lower end of the inner cavity of the diluter (1) is longitudinally and slidably provided with a dilution box (9); a temperature control lamp (8) is fixedly arranged on the side wall of the diluter (1) at the position of the dilution box (9), and the temperature control lamp (8) is electrically connected with a controller in the control panel (6); a control blocking piece (10) is vertically arranged in the middle of the mother liquor box (7); a weighing table (11) is fixedly arranged at the rear part of the bottom of the inner cavity of the diluter (1) in an embedded manner; guide grooves (101) are longitudinally formed in the positions, corresponding to the mounting positions of the mother liquor box (7), of the side walls at the left end and the right end of the inner cavity of the diluter (1); guide surplus grooves (102) are longitudinally formed in the side walls of the left end and the right end of the inner cavity of the diluter (1) and correspond to the dilution box (9), and the bottoms of the guide surplus grooves (102) are overlapped with the bottom plate of the inner cavity of the diluter (1); an upper sealing plate (104) is fixedly arranged at the rear part of the inner cavity of the diluter (1) above the position of the mother liquor box (7), and the bottom of the upper sealing plate (104) is in sliding tangency with the upper end of the mother liquor box (7); a middle partition plate (103) is fixedly arranged at the front part of the inner cavity of the diluter (1) below the mother liquor box (7), and a middle division hole (10301) is longitudinally formed in the middle of the middle partition plate (103); a stop slot (105) is formed in the middle of the bottom plane of the inner cavity of the diluter (1); the pumping pipe of the metering pump (5) extends into the inner cavity of the diluent storage box (4); the output end of the metering pump (5) is connected with a conveying main pipe (501), the conveying main pipe (501) is connected with two conveying branch pipes (503), an electric control valve (502) is arranged at the joint of the conveying main pipe (501) and the conveying branch pipes (503), the two conveying branch pipes (503) penetrate through the left side and the right side of the diluter (1) respectively and are arranged above the diluting box (9), the left conveying branch pipe and the right conveying branch pipe (503) are arranged above the diluting box (9) in a staggered mode, the tail ends of the conveying branch pipes (503) are of arc structures, the tail ends of the left conveying branch pipe and the right conveying branch pipe (503) are in opposite states, and the arc parts of the conveying branch pipes (503) are in inclined downward overturning states; guide wings A (701) are fixedly arranged on the side walls of the left end and the right end of the mother solution box (7), and the guide wings A (701) are slidably arranged in the guide grooves (101); a rectangular lower liquid groove (702) is formed in the middle of the mother liquid box (7), and a rectangular central hole (70202) penetrating up and down is formed in the middle of the bottom of the lower liquid groove (702); a round liquid discharging hole (70201) is vertically formed in the bottom of the liquid discharging groove (702) around the central hole (70202); longitudinal guide fins B (901) are fixedly arranged on the side walls of the left end and the right end of the dilution box (9), and the upper end faces of the guide fins B (901) are tangential to the upper groove walls of the guide surplus grooves (102); a disc-shaped magnetic supporting disc (902) is vertically and fixedly arranged upwards in the middle of the dilution box (9), and an arc-shaped diversion wing (90201) is arranged on the annular part on the upper end surface of the magnetic supporting disc (902); a pulling plate (903) is fixedly arranged in the middle of the side wall of the front end of the dilution box (9); the middle part of the pull plate (903) is vertically and slidably provided with a stop pin (90301), and when the dilution box (9) is arranged in the dilution box, the lower end of the stop pin (90301) falls into the stop slot hole (105); the control blocking piece (10) is of a rectangular structure, the control blocking piece (10) is consistent with the structure of the lower liquid groove (702), when the control blocking piece (10) is embedded in the lower liquid groove (702), the upper end face of the control blocking piece (10) is flush with the bottom plane of the inner cavity of the mother liquor box (7), the middle part of the bottom plane of the control blocking piece (10) is a vertically downward rectangular central column (1001), and the central column (1001) is inserted into the central hole (70202) in a penetrating manner; the lower end of the center column (1001) is fixedly connected with a disc-shaped magnetic bottom support filter disc (1002), the upper end of the magnetic bottom support filter disc (1002) is of a concave cambered surface structure, and the magnetic bottom support filter disc (1002) is made of a magnet with the same polarity as the magnetic bottom support disc (902); a stop ring (100101) is fixedly arranged on a center column (1001) below the mother liquor tank (7), blocking heads (1003) are distributed on the bottom plane of the control blocking piece (10) corresponding to the lower liquor holes (70201), and when the control blocking piece (10) moves upwards, the stop ring (100101) is contacted with the bottom of the mother liquor tank (7), and the blocking heads (1003) are separated from the lower liquor holes (70201); the weighing platform (11) detects a stable weight signal and then transmits the stable weight signal to a controller in the control panel (6), and the controller transmits the stable weight signal to the metering pump (5) and the electric control valve (502) to convey diluent with corresponding proportion to the diluent box (9).
2. A bacterial cell filtration diluter according to claim 1, wherein: a transparent glass liquid level window (401) is embedded in the back side wall of the diluent storage box (4).
3. The method of using a bacterial cell filtration diluter of claim 1, wherein:
firstly, opening an upper sealing door (3), pulling out a mother liquor box (7), injecting bacteriophage mother liquor into the mother liquor box (7), and adding bacteriophage mother liquor into the mother liquor box (7);
secondly, the mother liquor box (7) is pushed inwards to reset, as the polarities of the magnetic bearing tray (902) and the magnetic base filter tray (1002) are consistent, after alignment, the magnetic base filter tray (1002) is jacked up, the blocking piece (10) is controlled to ascend, phage mother liquor flows onto the magnetic base filter tray (1002) from the lower liquor hole (70201) and overflows onto the magnetic bearing tray (902) from the edge of the magnetic base filter tray (1002), in the process, impurities in phage mother liquor stay in the lower concave parts of the inner grooves of the magnetic base filter tray (1002), and the flowing phage mother liquor is uniformly distributed in the dilution box (9) in a rotary scattering state after being guided by the guide fins (90201);
finally, the weighing table (11) detects a weight change signal at the moment, after the weight is stable, the weight value is transmitted to the controller for processing, the controller transmits the diluent with corresponding proportion to the metering pump (5) and the electric control valve (502) to the diluent box (9), the phage mother liquor is diluted, after the phage mother liquor is cultured, the lower sealing door (2) is opened, and the diluent box (9) is pulled out for transferring and storing after the phage mother liquor is diluted.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202310157580.4A CN116144486B (en) | 2023-02-23 | 2023-02-23 | Thallus filtering diluter and use method |
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| CN202310157580.4A CN116144486B (en) | 2023-02-23 | 2023-02-23 | Thallus filtering diluter and use method |
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| CN116144486B true CN116144486B (en) | 2024-04-09 |
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| CN215250810U (en) * | 2021-07-20 | 2021-12-21 | 青岛诺安百特生物技术有限公司 | Filtering and diluting device for bacteriophage |
| CN114307703A (en) * | 2022-01-07 | 2022-04-12 | 李雨洁 | Efficient papermaking auxiliary agent diluting device for papermaking |
| CN217709444U (en) * | 2022-07-18 | 2022-11-01 | 山东新航线生物科技有限公司 | A filter diluting device for bacteriophage |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1324093A (en) * | 1969-07-18 | 1973-07-18 | Demarque D | Automatic apparatus permitting highly dilute solutions to be obtained |
| CN104266895A (en) * | 2014-10-11 | 2015-01-07 | 沈佳特 | Automatic continuous multi-stage dilution device for bacterial suspension and real-time counting method |
| JP2019018163A (en) * | 2017-07-19 | 2019-02-07 | 株式会社星光技研 | Automatic dilution apparatus and spray device |
| CN215140922U (en) * | 2021-03-19 | 2021-12-14 | 衢州鑫盛轴承有限公司 | Centralized filtering device for cooling liquid in bearing grinding |
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| CN116144486A (en) | 2023-05-23 |
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