CN116144484A - Automatic carbon dioxide incubator of rotation type - Google Patents
Automatic carbon dioxide incubator of rotation type Download PDFInfo
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- CN116144484A CN116144484A CN202211600039.8A CN202211600039A CN116144484A CN 116144484 A CN116144484 A CN 116144484A CN 202211600039 A CN202211600039 A CN 202211600039A CN 116144484 A CN116144484 A CN 116144484A
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Abstract
The invention relates to a rotary automatic carbon dioxide incubator, which comprises an incubator body, a controller, a fan, a first heating wire module, a temperature sensor, a culture layer module and a wireless temperature sensor, wherein the incubator body is provided with a first heating wire and a second heating wire; the device comprises a second heating wire module, a gas mixing box, a first carbon dioxide sensor, a first air pump, a second air pump, a gas detection box, a first carbon dioxide sensor and a second carbon dioxide sensor; the controller controls the fan to rotate according to a certain frequency to realize air flow circulation in the incubator body, achieves the basis that the first heating module achieves the preheating temperature through a pid control method, and then controls the second heating wire module to heat so as to achieve the temperature requirement required to be achieved in each incubator; the first air pump and the first air pump are controlled to work so as to meet the carbon dioxide concentration requirement required to be achieved in each culture bin, and the incubator furthest improves the space utilization rate of the instrument, the independence of different types of cells and the culture safety.
Description
Technical Field
The invention belongs to the field of full-automatic products, and relates to a rotary automatic carbon dioxide incubator.
Background
With the recent development of related fields such as biology and regenerative medicine, there is an increasing market demand for a carbon dioxide incubator capable of stably and efficiently culturing cells. How to simultaneously meet the requirements of culture quantity, culture safety and simultaneous culture of different types of cells becomes a problem to be solved urgently.
Disclosure of Invention
In order to solve the problems, the invention provides the following technical scheme: a rotary automatic carbon dioxide incubator comprises an incubator body and a controller;
the fan is arranged at the top of the incubator body and used for ensuring the circulation of air flow in the incubator body;
the first heating wire modules are arranged on the bottom of the incubator body and the walls of the surrounding incubator body and used for heating the temperature inside the incubator body so that the temperature of the incubator body reaches a preset temperature;
meanwhile, temperature sensors are respectively arranged on the bottom of the box body and the wall of the box body around the box body;
a first support column is arranged in the middle of the box body;
n culture layer modules capable of accommodating m culture cabins are arranged on the arrangement column layer by layer;
a wireless temperature sensor for collecting the temperature in the culture cabin is arranged in the culture cabin;
the bottom of the culture cabin is provided with a second heating wire module for heating the temperature in the culture cabin;
an air inlet and an air outlet for placing a carbon dioxide air inlet pipeline are formed in the top of the incubator body;
a gas mixing box for providing carbon dioxide gas with a certain concentration for the culture cabin;
a first carbon dioxide sensor for collecting the concentration value of carbon dioxide in the gas mixing box is arranged in the gas mixing box;
a first air pump for supplying power for pumping carbon dioxide gas with a certain concentration into the culture cabin;
a second air pump for pumping out the carbon dioxide gas in the culture cabin to provide power;
a gas detection tank that receives carbon dioxide gas extracted by the second air pump;
a first carbon dioxide sensor for collecting the concentration of carbon dioxide is arranged in the gas mixing box;
a second carbon dioxide sensor for collecting the concentration of carbon dioxide is arranged in the gas detection box;
the controller receives temperature signals transmitted by wireless temperature sensors in the culture cabin and temperature signals transmitted by the temperature sensors respectively, controls the fan to rotate according to a certain frequency to realize air flow circulation in the incubator body, realizes the basis that the first heating module reaches the preheating temperature through a pid control method, and controls the second heating wire module to heat so as to realize the temperature requirement needed to be reached in each culture cabin;
the controller receives the carbon dioxide concentration signals transmitted by the first carbon dioxide sensor and the second carbon dioxide sensor, and controls the first air pump to work with the first air pump so as to realize the carbon dioxide concentration requirement required to be reached in each culture bin.
Further: the n is more than or equal to 1 and less than or equal to 4, and the m is more than or equal to 1 and less than or equal to 4.
Further: the gas detection device further comprises an electromagnetic valve and a one-way valve, wherein the electromagnetic valve is connected with the controller and the first air pump, and the one-way valve is arranged on a gas path of the gas detection box.
Further: the controller receives the carbon dioxide concentration signals transmitted by the first carbon dioxide sensor and the second carbon dioxide sensor, and controls the first air pump to work with the first air pump to realize the process of meeting the carbon dioxide concentration requirement in each culture bin as follows:
when the difference I between the real-time concentration value detected by the second carbon dioxide sensor in the gas detection box and the set value is more than or equal to 3.5%, the controller controls the first air pump and the second air pump to work simultaneously; up to CO in the gas detection tank 2 The concentration reaches the set concentration;
when the second carbon dioxide sensor in the gas detection box detects the difference I between the real-time concentration value and the set value, 3.5 percent>I>At 0.5%, the first air pump at the inlet of the incubator is turned on for 60S, then turned off, at this time, the first air pump at the inlet of the gas detection box is turned on for 20S, then turned off, and the step of turning on the first air pump at the inlet of the incubator for 60S is repeated until the gasDetecting CO in a tank 2 When the sensor detects that the concentration real-time value is less than or equal to 0.5% of the set value difference I, the air pump at the air inlet of the culture cabin is connected with 15S, then the air pump at the air detection box inlet is disconnected, at the moment, the air pump at the air detection box inlet is connected with 3S, then the air pump at the air inlet of the culture cabin is disconnected, and the air pump at the air inlet of the culture cabin is repeatedly connected with 15S until the CO in the air detection box is detected 2 The concentration reached the set concentration.
Further, the method further comprises the following steps:
a motor module for controlling the culture layer module to rotate is arranged on the first support column;
the side surface of the box body is provided with a grabbing device for grabbing the culture cabin;
a transfer port for sending the grabbing culture bin to be grabbed by the grabbing device is arranged on the side face of the incubator body;
the controller acquires signals of the culture cabin to be grasped, and controls the grasping module to grasp the corresponding culture cabin and deliver the culture cabin from the transmission port.
Further: the grabbing device comprises a second support column arranged on the side surface of the interior of the incubator body;
a vertically moving electric cylinder is arranged on the second support column;
the grabbing clamp is connected with the electric cylinder and used for grabbing the culture bin;
the power module is used for controlling the grabbing clamp to horizontally move;
the power module comprises a gear and a servo motor, and the servo motor is connected with the gear.
The incubator of the rotary transfer system provided by the invention can accurately measure and control CO in the incubator 2 Concentration of CO 2 The power supply line and the signal line of the sensor influence the transmission of the culture cabin; and the hierarchical heating control mode improves the space utilization rate of the instrument, the independence of different types of cells and the culture safety to the maximum extent.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are required in the embodiments or the description of the prior art will be briefly described, and it is obvious that the drawings in the following description are some embodiments of the present invention, and other drawings may be obtained according to the drawings without inventive effort to a person skilled in the art.
FIG. 1 is a schematic view of the internal structure of the present invention;
FIG. 2 is a schematic view of the appearance structure of the present invention;
FIG. 3 is a schematic view of the invention with the interior broken away;
FIG. 4 is a schematic diagram of the layout of the surface heating wire of the present invention;
FIG. 5 is a schematic illustration of the storage rack and heater wire layout of the present invention;
FIG. 6 is a schematic diagram of the air path control of the present invention.
Reference numerals: 1. culture cabin, 2, snatch anchor clamps, 3, electric jar, 4, first support column, 5, motor, 6, transfer mouth, 7, air inlet, 8, gas outlet, 10, wireless temperature sensor, 11, incubator box, 12, first heater strip module, 13, culture layer module, 14, second heater strip module, 16, first air pump, 17, check valve, 18, busbar, 19, gas filter, 20, second carbon dioxide sensor.
Detailed Description
It should be noted that, without conflict, the embodiments of the present invention and features in the embodiments may be combined with each other, and the present invention will be described in detail below with reference to the drawings and the embodiments.
For the purpose of making the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is apparent that the described embodiments are only some embodiments of the present invention, not all embodiments. The following description of at least one exemplary embodiment is merely exemplary in nature and is in no way intended to limit the invention, its application, or uses. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of exemplary embodiments according to the present invention. As used herein, the singular is also intended to include the plural unless the context clearly indicates otherwise, and furthermore, it is to be understood that the terms "comprises" and/or "comprising" when used in this specification are taken to specify the presence of stated features, steps, operations, devices, components, and/or combinations thereof.
The relative arrangement of the components and steps, numerical expressions and numerical values set forth in these embodiments do not limit the scope of the present invention unless it is specifically stated otherwise. Meanwhile, it should be clear that the dimensions of the respective parts shown in the drawings are not drawn in actual scale for convenience of description. Techniques, methods, and apparatus known to those of ordinary skill in the relevant art may not be discussed in detail, but are intended to be part of the specification where appropriate. In all examples shown and discussed herein, any specific values should be construed as merely illustrative, and not a limitation. Thus, other examples of the exemplary embodiments may have different values. It should be noted that: like reference numerals and letters denote like items in the following figures, and thus once an item is defined in one figure, no further discussion thereof is necessary in subsequent figures.
In the description of the present invention, it should be understood that the azimuth or positional relationships indicated by the azimuth terms such as "front, rear, upper, lower, left, right", "lateral, vertical, horizontal", and "top, bottom", etc., are generally based on the azimuth or positional relationships shown in the drawings, merely to facilitate description of the present invention and simplify the description, and these azimuth terms do not indicate and imply that the apparatus or elements referred to must have a specific azimuth or be constructed and operated in a specific azimuth, and thus should not be construed as limiting the scope of protection of the present invention: the orientation word "inner and outer" refers to inner and outer relative to the contour of the respective component itself.
Spatially relative terms, such as "above … …," "above … …," "upper surface at … …," "above," and the like, may be used herein for ease of description to describe one device or feature's spatial location relative to another device or feature as illustrated in the figures. It will be understood that the spatially relative terms are intended to encompass different orientations in use or operation in addition to the orientation depicted in the figures. For example, if the device in the figures is turned over, elements described as "above" or "over" other devices or structures would then be oriented "below" or "beneath" the other devices or structures. Thus, the exemplary term "above … …" may include both orientations of "above … …" and "below … …". The device may also be positioned in other different ways (rotated 90 degrees or at other orientations) and the spatially relative descriptors used herein interpreted accordingly.
In addition, the terms "first", "second", etc. are used to define the components, and are only for convenience of distinguishing the corresponding components, and the terms have no special meaning unless otherwise stated, and therefore should not be construed as limiting the scope of the present invention.
FIG. 1 is a schematic view of the internal structure of the present invention;
FIG. 2 is a schematic view of the appearance structure of the present invention;
FIG. 3 is a schematic view of the invention with the interior broken away;
a rotary automatic carbon dioxide incubator control system, which comprises an incubator body 11 and a controller;
the fan is arranged at the top of the incubator body and used for ensuring the circulation of air flow in the incubator body 11;
the fan adopts a Hepa large-sized fan;
the first heating wire modules 12 for heating the temperature inside the incubator body and enabling the temperature of the incubator body 11 to reach the preset temperature are arranged at the bottom of the incubator body and on the wall of the surrounding incubator bodies;
FIG. 4 is a schematic diagram of the layout of the surface heating wire of the present invention;
FIG. 5 is a schematic illustration of the storage rack and heater wire layout of the present invention;
the first heating wire module 12 comprises a bottom heating wire sub-module, a left heating wire sub-module, a right heating wire sub-module, a front heating wire sub-module and a rear heating wire sub-module;
the power of the heating wires of the bottom heating wire sub-module, the left heating wire sub-module, the right heating wire sub-module, the front heating wire sub-module and the rear heating wire sub-module is 320W;
the bottom heating wire sub-module, the left heating wire sub-module, the right heating wire sub-module, the front heating wire sub-module and the rear heating wire sub-module are connected with the controller;
meanwhile, temperature sensors are respectively arranged on the bottom of the box body and the wall of the box body around the box body;
the temperature sensor adopts a PT100 temperature sensor;
a support column is arranged in the middle of the box body; the support column is round;
n culture layer modules 13 capable of accommodating m culture cabins 1 are arranged on the arrangement column layer by layer; n is not less than 1 and not more than 4, and m is not less than 1 and not more than 4;
the culture layer module 13 comprises a storage bracket of each culture cabin 1; and a temporary transfer platform is arranged outside the incubator body 11.
All storage brackets constitute a large storage mechanism and are connected through cylindrical support columns.
The incubator body 11 can be divided into 4 culture layer modules 13 at most, and the culture layer modules 13 are divided into 4 areas in a bisecting mode, and 1 culture cabin 1 is placed in each area.
A wireless temperature sensor 10 for acquiring the temperature in the culture cabin 1 is arranged in the culture cabin 1; feeding back the internal temperature value of each culture cabin 1 in real time;
a second heating wire module 14 for heating the temperature in the culture cabin 1 is arranged at the bottom of the culture cabin 1;
the power of the heating wire of the second heating wire module 14 is 10W;
an air inlet 7 and an air outlet 8 for placing a carbon dioxide air inlet pipeline are arranged at the top of the culture cabin 1 body;
a gas mixing box for providing carbon dioxide gas with a certain concentration for the culture cabin 1;
CO 2 the gas firstly enters the gas mixing box, then is filtered by the gas filter 19 and then enters the bus bar 18, and the bus bar 18 has the function of dividing a plurality of gas path interfaces and providing gas for a plurality of branches;
a first carbon dioxide sensor for collecting the concentration value of carbon dioxide in the gas mixing box is arranged in the gas mixing box;
a first air pump 16 for supplying power to the carbon dioxide gas of a certain concentration in the gas mixing box drawn into the culture chamber 1;
a second air pump for pumping out the carbon dioxide gas in the culture cabin 1 to provide power;
a gas detection tank that receives carbon dioxide gas extracted by the second air pump;
a first carbon dioxide sensor for collecting the concentration of carbon dioxide is arranged in the gas mixing box;
a second carbon dioxide sensor 20 for collecting carbon dioxide concentration is arranged in the gas detection box;
the device also comprises a solenoid valve and a one-way valve 17, wherein the solenoid valve is connected with the controller and the first air pump 16, and the one-way valve 17 is arranged on a gas path of the gas detection box.
The controller receives a temperature signal transmitted by the wireless temperature sensor 10 in the culture cabin 1 and a temperature signal transmitted by the temperature sensor, controls the fan to rotate according to a certain frequency to realize air circulation in the incubator body 11, realizes the basis that the first heating module reaches the preheating temperature through a pid control method, and controls the second heating wire module 14 to heat so as to realize the temperature requirement needed to be reached in each culture cabin;
when the temperature of the culture cabin 1 is set to 37 ℃, the heating wires of the bottom heating wire sub-module, the left heating wire sub-module, the right heating wire sub-module, the front heating wire sub-module and the rear heating wire sub-module are controlled to start working, and the internal temperature of the whole carbon dioxide culture cabin reaches more than 35 ℃ but not 37 ℃ through PID (proportion integration differentiation) adjustment and a top fan (the function of the fan mainly circulates the internal air flow of the whole instrument and ensures the internal temperature uniformity of the instrument to the greatest extent). At the same time, the second heating wire module 14 at the bottom of the culture cabin 1 starts to work, PID adjustment is performed according to the temperature value fed back by the wireless temperature sensor 10 in the culture cabin 1, and finally, the requirement of 37 ℃ is met by each culture cabin 1.
The controller receives the carbon dioxide concentration signals transmitted by the first carbon dioxide sensor and the second carbon dioxide sensor 20, and controls the first air pump 16 and the first air pump 16 to work so as to achieve the carbon dioxide concentration requirement required to be achieved in each cultivation bin.
According to the requirements CO set by each culture cabin 1 2 Concentration and real-time CO of each culture cabin 1 2 Concentration by CO in gas mixing box 2 The concentration value fed back by the sensor, and the controller controls the on-off of the electromagnetic valve and controls the CO according to the feedback value 2 Inflow of gases, e.g. CO mixed to a concentration of 10% in a gas-mixing box 2 A gas; CO based on the requirements in different culture tanks 1 2 The concentration value, control the air pump work, draw the gas in the gas mixing box into the culture cabin 1, draw the gas into the gas detection box from the gas outlet 8 of the culture cabin 1 through the second air pump, pass the CO in the gas detection box 2 Real-time CO measured by a sensor 2 The working frequency of the air pump is regulated, and meanwhile, the air outlet 8 of the air detection box discharges air back to the culture cabin 1 to form an air circulation loop. A one-way valve 17 is added in the air path of the air detection box to prevent the air from flowing back.
Further: the process of receiving the carbon dioxide concentration signals transmitted by the first carbon dioxide sensor and the second carbon dioxide sensor 20, and controlling the first air pump 16 and the first air pump 16 to work to achieve the carbon dioxide concentration requirement in each cultivating bin is as follows:
when the second carbon dioxide sensor 20 in the gas detection box detects that the concentration real-time value and the set value difference I are more than or equal to 3.5%, the controller controls the first air pump 16 and the second air pump to work simultaneously; up to CO in the gas detection tank 2 The concentration reaches the set concentration;
when the second carbon dioxide sensor 20 in the gas detection box detects the difference I between the real-time concentration value and the set value, 3.5 percent>I>0.5%, the first air pump 16 of the air inlet 7 of the culture cabin 1 is switched on for 60S, then switched off, at this time, the first air pump 16 of the air detection box inlet is switched on for 20S, then switched off, and then the step of switching on the first air pump 1660S of the air inlet 7 of the culture cabin 1 is repeated until the CO in the air detection box is detected 2 When the difference I between the real-time concentration value detected by the sensor and the set value is less than or equal to 0.5%, the air pump at the air inlet 7 of the culture cabin 1 is switched on for 15S, then switched off, at the moment, the air pump at the air detection box inlet is switched on for 3S, then switched off, and then the air pump at the air inlet 7 of the culture cabin 1 is repeatedly switched on for 15S until the CO in the air detection box is detected 2 The concentration reached the set concentration.
Further: comprises a motor module which is arranged on the support column and used for controlling the culture layer module 13 to rotate;
the motor module comprises a motor 5, the motor 5 adopts a stepping motor, and the controller controls the stepping motor to rotate, and 4 culture cabins 1 are aligned with the positions of the transfer ports 6 when the stepping motor rotates by 90 degrees;
a grabbing device for grabbing the culture cabin 1 is arranged on the side surface of the incubator body 11;
a transfer port 6 for sending the grabbing culture bin to be grabbed by the grabbing module is arranged on the side face of the incubator body 11;
the controller obtains the signal that needs to snatch the cultivation cabin, control motor module rotates and grabbing device snatchs the cultivation cabin that will snatch to send out through transfer port 6.
The incubator is further provided with a touch screen on the outer surface of the incubator body 11, different incubator cabins 1 are selected according to the touch screen, and the grabbing device grabs according to a motion control program, and the specific process is as follows:
firstly, according to the culture cabins with the designated numbers in the designated layers on the touch screen, the corresponding coordinates of each culture cabin on the horizontal plane are (0, 90.180.270), and according to the selected culture cabin 1, only the motor 5 is controlled to move corresponding steps to rotate the culture cabin 1 to the transmission port 6 for measurement. In the vertical direction, only 4 coordinates are needed, corresponding to 4 brackets, the electric cylinder 3 is controlled to move to the corresponding coordinates, and finally the mechanical arm is controlled to grasp.
The grabbing device comprises a second support column arranged on the side surface of the interior of the incubator body;
a vertically moving electric cylinder 3 is arranged on the second support column;
the grabbing clamp 2 is connected with the electric cylinder 3 and used for grabbing the culture bin;
a power module for controlling the grabbing clamp 2 to horizontally move;
the power module comprises a gear and a servo motor, and the servo motor is connected with the controller.
The gripping device satisfies the functions of storage, transfer and taking in of each culture cabin 1.
Further: the system also includes H 2 O 2 A sterilization unit;
the H is 2 O 2 The sterilization unit comprises a steam state H for generating and generating the requirement 2 O 2 The number of modules to be connected to each other is the same,
to be in the vapor state H 2 O 2 From the vapour state H 2 O 2 A piezoelectric pump for leading the generating module into the incubator body 11;
steam H in the incubator body 11 2 O 2 The gas path switching module is switched into the culture cabin 1 and simultaneously blocks CO2 gas from entering;
the culture cabin 1 is provided with a vapor state H removing device 2 O 2 Is provided;
the gas circuit switching module and the controlThe controller is connected with the air circuit switching module, the air circuit switching module is controlled to kill the culture cabin 1 according to the killing time, and after the killing time requirement is met, the exhaust electromagnetic valve is opened to remove the steam state H 2 O 2 。
When m=4 and n=4, the device comprises 16 culture cabin transfer storage mechanisms, 16 culture cabins, 1 controller and 16 culture cabins H 2 O 2 And a sterilization module.
Fig. 6 is a schematic diagram of the air path control of the present invention.
Finally, it should be noted that: the above embodiments are only for illustrating the technical solution of the present invention, and not for limiting the same; although the invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical scheme described in the foregoing embodiments can be modified or some or all of the technical features thereof can be replaced by equivalents; such modifications and substitutions do not depart from the spirit of the invention.
Claims (7)
1. The utility model provides an automatic carbon dioxide incubator of rotation type which characterized in that: comprises an incubator body and a controller;
the fan is arranged at the top of the incubator body and used for ensuring the circulation of air flow in the incubator body;
the first heating wire modules are arranged on the bottom of the incubator body and the walls of the surrounding incubator body and used for heating the temperature inside the incubator body so that the temperature of the incubator body reaches a preset temperature;
meanwhile, temperature sensors are respectively arranged on the bottom of the box body and the wall of the box body around the box body;
a first support column is arranged in the middle of the box body;
n culture layer modules capable of accommodating m culture cabins are arranged on the arrangement column layer by layer;
a wireless temperature sensor for collecting the temperature in the culture cabin is arranged in the culture cabin;
the bottom of the culture cabin is provided with a second heating wire module for heating the temperature in the culture cabin;
a gas mixing box for providing carbon dioxide gas with a certain concentration for the culture cabin;
a first carbon dioxide sensor for collecting the concentration value of carbon dioxide in the gas mixing box is arranged in the gas mixing box;
a first air pump for supplying power for pumping carbon dioxide gas with a certain concentration into the culture cabin;
a second air pump for pumping out the carbon dioxide gas in the culture cabin to provide power;
a gas detection tank that receives carbon dioxide gas extracted by the second air pump;
a first carbon dioxide sensor for collecting the concentration of carbon dioxide is arranged in the gas mixing box;
a second carbon dioxide sensor for collecting the concentration of carbon dioxide is arranged in the gas detection box;
the controller receives temperature signals transmitted by wireless temperature sensors in the culture cabin and temperature signals transmitted by the temperature sensors respectively, controls the fan to rotate according to a certain frequency to realize air flow circulation in the incubator body, realizes the basis that the first heating module reaches the preheating temperature through a pid control method, and controls the second heating wire module to heat so as to realize the temperature requirement needed to be reached in each culture cabin;
the controller receives the carbon dioxide concentration signals transmitted by the first carbon dioxide sensor and the second carbon dioxide sensor, and controls the first air pump to work with the first air pump so as to realize the carbon dioxide concentration requirement required to be reached in each culture bin.
2. A rotary automated carbon dioxide incubator according to claim 1, wherein: the n is more than or equal to 1 and less than or equal to 4, and the m is more than or equal to 1 and less than or equal to 4.
3. A rotary automated carbon dioxide incubator according to claim 1, wherein: the gas detection device further comprises an electromagnetic valve and a one-way valve, wherein the electromagnetic valve is connected with the controller and the first air pump, and the one-way valve is arranged on a gas path of the gas detection box.
4. A rotary automated carbon dioxide incubator according to claim 1, wherein: the top of the incubator body is provided with an air inlet and an air outlet for placing a carbon dioxide air inlet pipeline.
5. The rotary automated carbon dioxide incubator of claim 4, wherein: the controller receives the carbon dioxide concentration signals transmitted by the first carbon dioxide sensor and the second carbon dioxide sensor, and controls the first air pump to work with the first air pump to realize the process of meeting the carbon dioxide concentration requirement in each culture bin as follows:
when the difference I between the real-time concentration value detected by the second carbon dioxide sensor in the gas detection box and the set value is more than or equal to 3.5%, the controller controls the first air pump and the second air pump to work simultaneously; up to CO in the gas detection tank 2 The concentration reaches the set concentration;
when the second carbon dioxide sensor in the gas detection box detects the difference I between the real-time concentration value and the set value, 3.5 percent>I>When 0.5%, the first air pump at the air inlet of the culture cabin is connected with 60S and then disconnected, at the moment, the first air pump at the air detection box inlet is connected with 20S and then disconnected, and the step of connecting the first air pump at the air inlet of the culture cabin with 60S is repeated until the CO in the air detection box is detected 2 When the sensor detects that the concentration real-time value is less than or equal to 0.5% of the set value difference I, the air pump at the air inlet of the culture cabin is connected with 15S, then the air pump at the air detection box inlet is disconnected, at the moment, the air pump at the air detection box inlet is connected with 3S, then the air pump at the air inlet of the culture cabin is disconnected, and the air pump at the air inlet of the culture cabin is repeatedly connected with 15S until the CO in the air detection box is detected 2 The concentration reached the set concentration.
6. A rotary automated carbon dioxide incubator according to claim 1, wherein: comprising
The support column is provided with a motor module for controlling the culture layer module to rotate;
the side surface of the box body is provided with a grabbing device for grabbing the culture cabin;
a transfer port for sending the grabbing culture bin to be grabbed by the grabbing device is arranged on the side face of the incubator body;
the controller acquires signals of the culture cabin to be grasped, and controls the grasping module to grasp the corresponding culture cabin and deliver the culture cabin from the transmission port.
7. The rotary automated carbon dioxide incubator of claim 6, wherein: the grabbing device comprises a second support column arranged on the side surface of the interior of the incubator body;
a vertically moving electric cylinder is arranged on the second support column;
the grabbing clamp is connected with the electric cylinder and used for grabbing the culture bin;
the power module is used for controlling the grabbing clamp to horizontally move;
the power module comprises a gear and a servo motor, and the servo motor is connected with the gear.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211600039.8A CN116144484A (en) | 2022-12-13 | 2022-12-13 | Automatic carbon dioxide incubator of rotation type |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211600039.8A CN116144484A (en) | 2022-12-13 | 2022-12-13 | Automatic carbon dioxide incubator of rotation type |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN116144484A true CN116144484A (en) | 2023-05-23 |
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ID=86338071
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211600039.8A Pending CN116144484A (en) | 2022-12-13 | 2022-12-13 | Automatic carbon dioxide incubator of rotation type |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN116144484A (en) |
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2022
- 2022-12-13 CN CN202211600039.8A patent/CN116144484A/en active Pending
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