CN116144469A - Bifidobacterium culture equipment - Google Patents
Bifidobacterium culture equipment Download PDFInfo
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- CN116144469A CN116144469A CN202310414032.5A CN202310414032A CN116144469A CN 116144469 A CN116144469 A CN 116144469A CN 202310414032 A CN202310414032 A CN 202310414032A CN 116144469 A CN116144469 A CN 116144469A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/04—Flat or tray type, drawers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/52—Mobile; Means for transporting the apparatus
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/20—Degassing; Venting; Bubble traps
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
- C12M37/04—Seals
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/02—Separating microorganisms from the culture medium; Concentration of biomass
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
Abstract
The invention discloses a bifidobacterium culture device, which belongs to the technical field of bifidobacterium culture devices and comprises a culture device and a liquid adding device, wherein the liquid adding device is arranged at one side of the culture device; the culture device comprises a culture turnplate, a culture support cylinder, a culture rotating column, a lifting sliding sleeve, a magnetic turnplate, a plurality of culture plates and a slide way, wherein the culture support cylinder is fixedly connected to the top of the culture turnplate, the culture rotating column is rotationally connected with the top of the culture turnplate, the culture rotating column is arranged in the culture support cylinder, the lifting sliding sleeve is in sliding connection with the culture rotating column, the magnetic turnplate is rotationally connected with the top of the culture rotating column, the slide way is symmetrically arranged on two sides of the culture support cylinder, the magnetic turnplate is in clamping connection with the culture support cylinder, and the culture plates are in sliding connection with the slide way; solves the problems of poor controllability, poor culture effect and the like of the bifidobacterium culture device in most centralized culture.
Description
Technical Field
The invention relates to the technical field of bifidobacteria, in particular to bifidobacteria culture equipment.
Background
The bifidobacterium is a probiotics, can effectively increase beneficial bacteria in intestinal tracts, has very important effect on maintaining microecological balance of the intestinal tracts, and is mainly prepared into tablets, dry powder or capsule preparations by using a microbial fermentation tank through the production processes of bacterial liquid culture, proportion blending, inoculation fermentation, freeze drying and the like.
The bifidobacteria play an important role in human health, and the number of the bifidobacteria in the intestinal tract of the infant in lactation period can reach about 90% of the number of the bacterial groups according to statistics, and the number of the bacterial groups starts to be gradually reduced along with the increase of the age, so that the number of the bifidobacteria is even reduced to about 7% for the aged over 65 years. In addition, the bifidobacterium also has the functions of reducing endotoxin, protecting liver, resisting tumor, enhancing immunity, scavenging free radicals, resisting aging and the like. As bifidobacteria have severe requirements on growth environment, strains are difficult to culture and have large mass production difficulty, the existing method generally adopts a microorganism fermentation tank fed-batch culture mode to carry out pharmaceutical production.
Chinese patent publication No. CN113583859a discloses an oxygen-tolerant bifidobacterium fermentation apparatus; the second cultivates the jar setting in one side of first cultivateing jar, water pump and first cultivate jar fixed connection, and be located the top of water pump, the one end and the water pump fixed connection of feed liquor pipe, the other end of feed liquor pipe sets up in the bottom of first cultivates jar, the one end and the water pump fixed connection of transfer pipe, the other end of transfer pipe sets up in the top of second cultivates jar, the inside of first cultivates jar and second cultivates jar all is provided with the control by temperature change subassembly, cultivate the jar separately to bifidobacterium through first cultivateing jar and second cultivates jar, bifidobacterium cultivates the jar after 12 hours with its second cultivateing jar through transmission component, rethread alkali regulation PH value, two cultivate the jar simultaneous culture, and accord with the growth condition of bifidobacterium more.
Chinese patent publication No. CN113583859a discloses an oxygen-tolerant bifidobacterium fermentation apparatus; the second cultivates the jar setting in one side of first cultivateing jar, water pump and first cultivate jar fixed connection, and be located the top of water pump, the one end and the water pump fixed connection of feed liquor pipe, the other end of feed liquor pipe sets up in the bottom of first cultivates jar, the one end and the water pump fixed connection of transfer pipe, the other end of transfer pipe sets up in the top of second cultivates jar, the inside of first cultivates jar and second cultivates jar all is provided with the control by temperature change subassembly, cultivate the jar separately to bifidobacterium through first cultivateing jar and second cultivates jar, bifidobacterium cultivates the jar after 12 hours with its second cultivateing jar through transmission component, rethread alkali regulation PH value, realize two cultivates jar simultaneous culture.
However, the existing bifidobacterium culture devices are mostly used for centralized culture, so that the controllability in the culture process is poor, and the culture effect is poor.
Disclosure of Invention
The invention aims to provide bifidobacterium culture equipment, and aims to solve the problems that most of the existing bifidobacterium culture devices in the prior art are centralized in culture, poor in controllability and poor in culture effect in the culture process.
The invention is realized in that a bifidobacterium culture device comprises a culture device and a liquid adding device, wherein the liquid adding device is arranged at one side of the culture device;
the culture device comprises a culture turnplate, a culture support cylinder, a culture rotating column, a lifting sliding sleeve, a magnetic turnplate, a plurality of culture plates and a slide way, wherein the culture support cylinder is fixedly connected to the top of the culture turnplate, the culture rotating column is rotationally connected with the top of the culture turnplate, the culture rotating column is arranged in the culture support cylinder, the lifting sliding sleeve is in sliding connection with the culture rotating column, the magnetic turnplate is rotationally connected with the top of the culture rotating column, the slide way is symmetrically arranged on two sides of the culture support cylinder, the magnetic turnplate is in clamping connection with the culture support cylinder, and the culture plates are in sliding connection with the slide way;
the liquid adding device comprises an auxiliary clamping seat and a liquid adding head, wherein the liquid adding head is arranged in the middle of the auxiliary clamping seat, and the auxiliary clamping seat is clamped with the culture disc.
The culture dish comprises a clamping block, a turnover block, a bin body support, a travel rod and a bin body, wherein the clamping block is rotationally connected with the bin body support through the turnover block, one end of the bin body support, far away from the turnover block, is fixedly connected with the bin body, the travel rod is fixedly connected on the clamping block, a travel stop block is fixedly connected with the side wall of the bin body support and abuts against the travel rod, and the end part of the clamping block abuts against the top of the lifting sliding sleeve;
the bin body comprises a bin body slide way, a bin body spring, a bin body support plate, a one-way valve and a leakage-proof lock plate, wherein the bin body slide way is symmetrically arranged on two side walls of the bin body, the bin body support plate is in sliding connection with the bin body slide way, the bin body spring is arranged in the bin body slide way, the bottom of the bin body spring is propped against the bin body support plate, the one-way valve is arranged in the middle of the bin body support plate, and the leakage-proof lock plate is arranged at the top of the bin body.
The clamping block comprises an inner clamping plate, an outer clamping plate and a vertical clamping plate, wherein the clamping plates are symmetrically fixedly connected to two sides of the clamping block, the outer clamping plate is fixedly connected to the outer side of the clamping block, the vertical clamping plate is symmetrically arranged at the top and the bottom of the outer clamping plate, and the middle of the inner clamping plate and the middle of the outer clamping plate are clamped and connected with the culture support tube.
The auxiliary clamping seat is characterized in that two slidable auxiliary clamping rods are symmetrically arranged in the middle of the auxiliary clamping seat, the two auxiliary clamping rods are connected through auxiliary springs, auxiliary buckles are arranged at the bottoms of the auxiliary clamping rods, and a slidable telescopic pipe is arranged in the middle of the liquid adding head.
The liquid feeder still includes the liquid feeding frame, liquid feeding frame top rigid coupling has the liquid feeding extension board, liquid feeding extension board top rigid coupling has the liquid feeding cylinder, liquid feeding cylinder bottom is equipped with telescopic liquid feeding gas pole and supplementary cassette top rigid coupling, one side rigid coupling that liquid feeding extension board was kept away from to the liquid feeding frame has the stock solution storehouse, stock solution storehouse top rigid coupling has the liquid pushing cylinder, liquid pushing gas cylinder bottom is equipped with telescopic liquid pushing gas pole, liquid feeding gas pole non-flexible end and liquid pushing plate rigid coupling, one side that liquid feeding frame kept away from the stock solution storehouse is equipped with the liquid feeding pipe, liquid feeding pipe both ends respectively with stock solution storehouse and liquid feeding head intercommunication.
Still include the liquid trap, the liquid trap includes liquid collecting seat, liquid collecting rotary column, liquid collecting carousel, liquid collecting pipe, liquid collecting mouth and liquid collecting push rod, the liquid collecting carousel rotates with the liquid collecting seat through liquid collecting rotary column to be connected, the rigid coupling has the liquid collecting pipe of a plurality of annular equipartitions on the liquid collecting carousel, liquid collecting pipe top rigid coupling has the liquid collecting mouth, liquid collecting mouth middle part rigid coupling has the liquid collecting push rod.
The device also comprises temperature adjusting bins which are symmetrically arranged at two sides of the culture rotating column, wherein temperature adjusting frames are fixedly connected at two sides of the inner wall of the temperature adjusting bins, detachable temperature adjusting pipes are arranged on the temperature adjusting frames, and air discharging blocks are fixedly connected at the top of the temperature adjusting bins.
The culture medium also comprises a base and a moving block, wherein the base is of a cross plate-shaped structure, the bottom of the culture turntable is rotationally connected with the middle part of the base, the moving block is symmetrically arranged at the top of the base, and the liquid adding device, the temperature adjusting bin and the liquid collector are fixedly connected at the top of the moving block;
the movable block comprises a movable slide rail, a movable slide block, a movable seat, a movable screw and a movable motor, wherein the movable slide rail is symmetrically arranged at the top of the base, the movable seat is in sliding connection with the movable slide block through the movable slide block, the movable motor is fixedly connected to one end of the base, which is far away from the culture turntable, the movable screw is rotationally connected with the movable motor, and the movable screw is in threaded transmission connection with the movable seat.
Compared with the prior art, the invention has the following beneficial effects:
the invention provides a rotatable incubator and a liftable liquid feeder, a plurality of culture trays are arranged in the incubator for culturing bifidobacteria, the operations of inputting culture liquid, temperature-adjusting culture, collecting and the like are completed by rotating the incubator and driving the culture trays, the plurality of culture trays are used for carrying out tray-by-tray culture, the controllability and the high quality of culturing the bifidobacteria are improved, the contact with air is isolated in the inputting, temperature-adjusting culture and collecting processes, and the deterioration or failure of the bifidobacteria caused by the contact of excessive air is avoided, and the processing efficiency and the processing quality are influenced.
The invention provides a culture tray as a culture device of bifidobacteria, a culture place for isolating oxygen is provided in the culture process, a bin body support plate provides a plurality of functions of extruding air before culture, isolating oxygen during culture, extruding liquid after culture and the like for the bin body under the assistance of a bin body slideway and a bin body spring, and the versatility and the culture stability of the device are improved. The interior cardboard and outer card board provide horizontal joint at joint piece lift in-process, and perpendicular cardboard is at the joint piece along being close to the gliding in-process of the slide that the carousel was inhaled to magnetism department to the joint piece, and the joint piece is close to cultivateing rotary column one end and is equipped with the magnetism and inhale the piece, when the joint piece removes to cultivateing rotary column top, receives magnetism carousel to adsorb, drives the joint piece and rotates the transposition, has improved the volume of holding and cultivation volume of the device. In the process of adding liquid, the auxiliary clamping seat clamps the culture dish, so that the liquid adding head can be used for adding liquid rapidly, and the liquid adding stability and liquid adding efficiency are improved.
The liquid adding device is provided with a liquid storage bin for storing culture stock solution, and is used for delivering the culture stock solution in an extrusion mode, and the extension of the telescopic pipe is completed, so that the convenience and durability of the device are improved. The liquid collector rotates in sequence through the liquid collecting pipes to lean against and push the valve core of the one-way valve, so that liquid in the culture dish flows into the liquid collecting pipes to collect liquid, and the operation completely isolates oxygen, so that the processing efficiency of the device is improved. The temperature regulating bin completes the temperature regulating operation of the temperature regulating bin by controlling the temperature of the temperature regulating pipe and the closing of the air exhaust block, and the culture efficiency of the bifidobacterium is improved.
Drawings
FIG. 1 is a schematic view of a bifidobacterium cultivation apparatus according to the present invention (view angle I);
FIG. 2 is a schematic diagram of a bifidobacterium cultivation apparatus according to the present invention (view II);
FIG. 3 is a schematic diagram showing the structure of a liquid filler in a bifidobacterium cultivation apparatus according to the present invention;
FIG. 4 is an enlarged view of a portion of the portion A of FIG. 3;
FIG. 5 is a schematic view showing the structure of an incubator in a bifidobacterium cultivation apparatus according to the present invention;
FIG. 6 is a schematic view showing the structure of a culture tray in a bifidobacterium culture apparatus according to the present invention;
FIG. 7 is a schematic diagram showing the structure of a liquid trap in a bifidobacterium cultivation apparatus according to the present invention.
Reference numerals:
base 1, movable block 2, incubator 3, liquid filler 4, temperature adjustment bin 5, liquid collector 6, incubation turntable 7, incubation support 8, incubation column 9, lifting slide sleeve 10, magnetic turntable 11, incubation disk 12, slide 13, clamping block 14, inner clamping plate 15, outer clamping plate 16, vertical clamping plate 17, turnover block 18, bin support 19, travel rod 20, travel stop 21, bin 22, bin slide 23, bin spring 24, bin support 25, check valve 26, leak-proof lock plate 27, liquid storage bin 28, liquid pushing cylinder 29, liquid pushing rod 30, liquid pushing plate 31, liquid feeding support plate 32, liquid feeding tube 33, liquid feeding frame 34, liquid feeding cylinder 35, liquid feeding rod 36, auxiliary clamping seat 37, auxiliary clamping rod 38, auxiliary spring 39, liquid feeding head 40, telescopic tube 41, auxiliary buckle 42, temperature adjustment frame 43, temperature adjustment tube 44, air discharging block 45, liquid collecting seat 46, liquid collecting rotary column 47, liquid collecting turntable 48, tube 49, liquid collecting port 50, push rod 51, moving slide 52, moving slide rail 53, moving screw rod 54, moving seat 55, and moving seat 55.
Detailed Description
The present invention will be described in further detail with reference to the drawings and examples, in order to make the objects, technical solutions and advantages of the present invention more apparent. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the scope of the invention.
The implementation of the present invention will be described in detail below with reference to specific embodiments.
The same or similar reference numerals in the drawings of the present embodiment correspond to the same or similar components; in the description of the present invention, it should be understood that, if there is an azimuth or positional relationship indicated by terms such as "upper", "lower", "left", "right", etc., based on the azimuth or positional relationship shown in the drawings, it is only for convenience of describing the present invention and simplifying the description, but it is not indicated or implied that the apparatus or element referred to must have a specific azimuth, be constructed and operated in a specific azimuth, and thus terms describing the positional relationship in the drawings are merely illustrative and should not be construed as limitations of the present patent, and specific meanings of the terms described above may be understood by those skilled in the art according to specific circumstances.
As shown in FIGS. 1 to 7, the present invention provides a bifidobacterium cultivation apparatus including a incubator 3 and a liquid feeder 4, the liquid feeder 4 being disposed at one side of the incubator 3;
the incubator 3 comprises an incubation rotary table 7, an incubation rotary table 8, incubation rotary columns 9, lifting sliding sleeves 10, a magnetic rotary table 11, a plurality of incubation trays 12 and a slideway 13, wherein the incubation rotary table 8 is fixedly connected to the top of the incubation rotary table 7, the incubation rotary columns 9 are rotationally connected with the top of the incubation rotary table 7, the incubation rotary columns 9 are arranged in the incubation rotary table 8, the lifting sliding sleeves 10 are slidingly connected with the incubation rotary columns 9, the magnetic rotary table 11 is rotationally connected with the top of the incubation rotary columns 9, the slideway 13 is symmetrically arranged on two sides of the incubation rotary table 8, the magnetic rotary table 11 is clamped with the incubation rotary table 8, and the plurality of incubation trays 12 are slidingly connected with the slideway 13;
the liquid adding device 4 comprises an auxiliary clamping seat 37 and a liquid adding head 40, wherein the liquid adding head 40 is arranged in the middle of the auxiliary clamping seat 37, and the auxiliary clamping seat 37 is clamped with the culture dish 12.
The invention is provided with the rotatable incubator 3 and the liftable liquid feeder 4, and a plurality of culture trays 12 are arranged in the incubator 3 for culturing bifidobacteria, and the operations of inputting, temperature-adjusting culturing, collecting and the like of the culture liquid are completed by rotating the incubator 3 and driving the culture trays 12, so that the controllability and high quality of culturing the bifidobacteria are improved by separating the trays for culturing the plurality of culture trays 12, and the contact with air is isolated in the inputting, temperature-adjusting culturing and collecting processes, so that the deterioration or failure of the bifidobacteria caused by the contact of the bifidobacteria with excessive air is avoided, and the processing efficiency and the processing quality are influenced.
The culture dish 12 comprises a clamping block 14, a turnover block 18, a bin body support 19, a travel rod 20 and a bin body 22, wherein the clamping block 14 is rotationally connected with the bin body support 19 through the turnover block 18, one end of the bin body support 19, which is far away from the turnover block 18, is fixedly connected with the bin body 22, the travel rod 20 is fixedly connected on the clamping block 14, a travel stop block 21 is fixedly connected with the side wall of the bin body support 19 and is abutted against the travel rod 20, and the end part of the clamping block 14 is abutted against the top of the lifting sliding sleeve 10;
the bin body 22 comprises a bin body slideway 23, a bin body spring 24, a bin body supporting plate 25, a one-way valve 26 and a leakage-proof lock plate 27, the bin body slideway 23 is symmetrically arranged on two side walls of the bin body 22, the bin body supporting plate 25 is slidably connected with the bin body slideway 23, the bin body spring 24 is arranged in the bin body slideway 23, the bottom of the bin body spring 24 is abutted against the bin body supporting plate 25, the one-way valve 26 is arranged in the middle of the bin body supporting plate 25, and the leakage-proof lock plate 27 is arranged at the top of the bin body 22.
The culture dish 12 is used as a bifidobacterium culture device, provides a culture place for isolating oxygen in the culture process, and the bin body support plate 25 provides a plurality of functions of extruding air before culture, isolating oxygen in the culture process, extruding liquid after culture and the like for the bin body 22 under the assistance of the bin body slide 23 and the bin body spring 24, so that the versatility and the culture stability of the device are improved.
The clamping block 14 comprises an inner clamping plate 15, an outer clamping plate 16 and a vertical clamping plate 17, wherein the clamping plate 15 is symmetrically and fixedly connected to two sides of the clamping block 14, the outer clamping plate 16 is fixedly connected to the outer side of the clamping block 14, the vertical clamping plate 17 is symmetrically arranged at the top and the bottom of the outer clamping plate 16, and the middle parts of the inner clamping plate 15 and the outer clamping plate 16 are clamped and connected with the culture support tube 8.
The inner clamping plate 15 and the outer clamping plate 16 provide transverse clamping in the lifting process of the clamping block 14, the vertical clamping plate 17 clamps the clamping block 14 in the sliding process of the clamping block 14 along the slideway 13 close to the magnetic rotary table 11, the magnetic rotary table is arranged at one end of the clamping block 14 close to the culture rotary column 9, and when the clamping block 14 moves to the top of the culture rotary column 9, the clamping block 14 is adsorbed by the magnetic rotary table 11 to drive the clamping block 14 to rotate and change positions, so that the accommodating capacity and the culturing capacity of the device are improved.
Two slidable auxiliary clamping rods 38 are symmetrically arranged in the middle of the auxiliary clamping seat 37, the two auxiliary clamping rods 38 are connected through an auxiliary spring 39, an auxiliary buckle 42 is arranged at the bottom of the auxiliary clamping rod 38, and a slidable telescopic tube 41 is arranged in the middle of the liquid adding head 40.
In the process of adding liquid, the auxiliary clamping seat 37 clamps the culture dish 12, so that the liquid adding head 40 can be used for adding liquid rapidly, and the liquid adding stability and liquid adding efficiency are improved.
The liquid charger 4 still includes liquid feeding frame 34, and liquid feeding frame 34 top rigid coupling has liquid feeding extension board 32, and liquid feeding extension board 32 top rigid coupling has liquid feeding cylinder 35, and liquid feeding cylinder 35 bottom is equipped with telescopic liquid feeding gas pole 36, liquid feeding gas pole non-flexible end and auxiliary clamping seat 37 top rigid coupling, and liquid feeding frame 34 is kept away from one side rigid coupling of liquid feeding extension board 32 and is had stock solution storehouse 28, and stock solution storehouse 28 top rigid coupling has liquid pushing cylinder 29, and liquid pushing cylinder 29 bottom is equipped with telescopic liquid pushing gas pole 30 and push plate 31 rigid coupling, and liquid feeding frame 34 is kept away from one side of stock solution storehouse 28 and is equipped with liquid feeding pipe 33, and liquid feeding pipe 33 both ends communicate with stock solution storehouse 28 and liquid feeding head 40 respectively.
The liquid feeder 4 is provided with a liquid storage bin 28 for storing culture stock solution, and is used for feeding liquid in an extrusion mode, and the extension of the telescopic tube 41 is completed, so that the convenience and durability of the device are improved.
Still include liquid collector 6, liquid collector 6 includes liquid collecting seat 46, liquid collecting rotary column 47, liquid collecting carousel 48, liquid collecting pipe 49, liquid collecting mouth 50 and liquid collecting push rod 51, and liquid collecting carousel 48 rotates with liquid collecting seat 46 through liquid collecting rotary column 47 to be connected, and the rigid coupling has the liquid collecting pipe 49 of a plurality of annular equipartitions on the liquid collecting carousel 48, and liquid collecting pipe 49 top rigid coupling has liquid collecting mouth 50, and liquid collecting mouth 50 middle part rigid coupling has liquid collecting push rod 51.
The liquid collector 6 rotates to abut against and push the valve core of the one-way valve 26 through the plurality of liquid collecting pipes 49 in sequence, so that liquid in the culture dish 12 flows into the liquid collecting pipes 49 to perform liquid collecting operation, and the operation completely isolates oxygen, so that the processing efficiency of the device is improved.
The device also comprises a temperature regulating bin 5, the temperature regulating bin 5 is symmetrically arranged at two sides of the culture rotating column 9, the two sides of the inner wall of the temperature regulating bin 5 are fixedly connected with temperature regulating frames 43, detachable temperature regulating pipes 44 are arranged on the temperature regulating frames 43, and an exhaust block 45 is fixedly connected at the top of the temperature regulating bin 5.
The temperature regulating bin 5 completes the temperature regulating operation of the temperature regulating bin 5 by controlling the temperature of the temperature regulating pipe 44 and the closing of the exhaust block 45, and the culturing efficiency of bifidobacteria is improved.
The culture medium also comprises a base 1 and a movable block 2, wherein the base 1 is of a cross plate-shaped structure, the bottom of the culture turntable 7 is rotationally connected with the middle part of the base 1, the movable block 2 is symmetrically arranged at the top of the base 1, and the liquid filler 4, the temperature-adjusting bin 5 and the liquid collector 6 are fixedly connected at the top of the movable block 2;
the movable block 2 comprises a movable slide rail 52, a movable slide block 53, a movable seat 54, a movable screw 55 and a movable motor 56, wherein the movable slide rail 52 is symmetrically arranged at the top of the base 1, the movable seat 54 is in sliding connection with the movable slide block 53, the movable motor 56 is fixedly connected to one end of the base 1 far away from the culture turntable 7, the movable screw 55 is in rotary connection with the movable motor 56, and the movable screw 55 is in threaded transmission connection with the movable seat 54.
The working principle of the invention is as follows:
the movable motor 56 is controlled to act, the movable screw 55 is driven to rotate, the movable block 2 is driven to move the liquid adding frame 34 towards the culture tray 12 until the liquid adding frame moves to the upper part of the culture tray 12, the culture tray 12 is driven by the lifting sliding sleeve 10 to move towards the auxiliary clamping seat 37, the auxiliary clamping rod 38 is opened after being pushed by the culture tray, the culture tray 12 is clamped under the pulling of the auxiliary spring 39, the liquid adding cylinder 35 is driven by the liquid adding air rod 36 to press the liquid pushing plate 31 to press the culture stock solution, the telescopic pipe 41 is driven to extend, the valve core of the one-way valve 26 is driven, the culture stock solution is injected into the bin 22, the bin support plate 25 is driven to move away from the bottom of the bin 22 by the pushing force, the bin spring 24 is stressed to compress, the lifting sliding sleeve 10 is driven to drive the culture tray 12 to move to the top of the culture rotating column 9 after the liquid injection is completed, the rear end of the clamping block 14 is adsorbed on the side edge of the magnetic rotating disc 11, after magnetism of the magnetic turntable 11 is removed, the clamping blocks 14 fall into the other adjacent slide ways to reciprocate until all culture trays 12 are filled with liquid, the culture turntable 7 is rotated to drive the culture trays 12 to rotate, the culture trays 12 are moved into the temperature-regulating bin 5 to be cultured, after the culture is completed, the culture turntable 7 is rotated to drive the culture trays 12 to rotate to the top of the liquid collector 6, the overturning of the culture trays 12 is completed through the rotating overturning blocks 18, the culture trays 12 are continuously close to the liquid collector 6, the one-way valves 26 are inserted into the liquid collecting ports 50, the liquid collecting push rods 51 push the valve cores of the one-way valves 26, the bin body support plates 25 are pushed by the bin body springs 24 to discharge liquid, and a plurality of operations such as air extrusion, oxygen-isolation culture in the culture process, liquid extrusion and the like are completed.
Referring to the drawings, there is shown a preferred embodiment of the present invention.
The foregoing description of the preferred embodiments of the invention is not intended to be limiting, but rather is intended to cover all modifications, equivalents, and alternatives falling within the spirit and principles of the invention.
Claims (8)
1. A bifidobacterium culture device comprising a culture vessel and a liquid feeder, wherein the liquid feeder is arranged at one side of the culture vessel;
the culture device comprises a culture turnplate, a culture support cylinder, a culture rotating column, a lifting sliding sleeve, a magnetic turnplate, a plurality of culture plates and a slide way, wherein the culture support cylinder is fixedly connected to the top of the culture turnplate, the culture rotating column is rotationally connected with the top of the culture turnplate, the culture rotating column is arranged in the culture support cylinder, the lifting sliding sleeve is in sliding connection with the culture rotating column, the magnetic turnplate is rotationally connected with the top of the culture rotating column, the slide way is symmetrically arranged on two sides of the culture support cylinder, the magnetic turnplate is in clamping connection with the culture support cylinder, the magnetic turnplate is in sliding connection with the slide way, and a plurality of culture plates are in sliding connection with the slide way;
the liquid adding device comprises an auxiliary clamping seat and a liquid adding head, wherein the liquid adding head is arranged in the middle of the auxiliary clamping seat, and the auxiliary clamping seat is clamped with the culture disc.
2. The bifidobacterium culture equipment according to claim 1, wherein the culture tray comprises a clamping block, a turnover block, a bin body support, a travel rod and a bin body, the clamping block is rotationally connected with the bin body support through the turnover block, one end of the bin body support, far away from the turnover block, is fixedly connected with the bin body, the travel rod is fixedly connected on the clamping block, a travel stop block is fixedly connected on the side wall of the bin body support and is abutted against the travel rod, and the end part of the clamping block is abutted against the top of the lifting sliding sleeve;
the bin body comprises a bin body slide way, a bin body spring, a bin body support plate, a one-way valve and a leakage-proof lock plate, wherein the bin body slide way is symmetrically arranged on two side walls of the bin body, the bin body support plate is in sliding connection with the bin body slide way, the bin body spring is arranged in the bin body slide way, the bottom of the bin body spring is propped against the bin body support plate, the one-way valve is arranged in the middle of the bin body support plate, and the leakage-proof lock plate is arranged at the top of the bin body.
3. The bifidobacterium culture apparatus according to claim 2, wherein the clamping block comprises an inner clamping plate, an outer clamping plate and a vertical clamping plate, the clamping plates are symmetrically and fixedly connected to two sides of the clamping block, the outer clamping plate is fixedly connected to the outer side of the clamping block, the vertical clamping plate is symmetrically arranged at the top and the bottom of the outer clamping plate, and the culture support is clamped between the inner clamping plate and the middle of the outer clamping plate.
4. A bifidobacterium cultivation apparatus as claimed in claim 3, wherein two auxiliary clamping bars are symmetrically arranged in the middle of the auxiliary clamping seat, the two auxiliary clamping bars are connected through auxiliary springs, auxiliary buckles are arranged at the bottoms of the auxiliary clamping bars, and a telescopic tube is arranged in the middle of the liquid adding head.
5. The bifidobacterium culture equipment according to claim 4, wherein the liquid feeder further comprises a liquid feeding frame, a liquid feeding support plate is fixedly connected to the top of the liquid feeding frame, a liquid feeding cylinder is fixedly connected to the top of the liquid feeding support plate, a telescopic liquid feeding air rod is arranged at the bottom of the liquid feeding cylinder, a non-telescopic end of the liquid feeding air rod is fixedly connected with the top of the auxiliary clamping seat, a liquid storage bin is fixedly connected to one side, far away from the liquid feeding support plate, of the liquid feeding frame, a liquid pushing cylinder is fixedly connected to the top of the liquid storage bin, a telescopic liquid pushing air rod is arranged at the bottom of the liquid pushing cylinder and fixedly connected with the liquid pushing plate, a liquid feeding pipe is arranged at one side, far away from the liquid storage bin, of the liquid feeding frame, and two ends of the liquid feeding pipe are respectively communicated with the liquid storage bin and the liquid feeding head.
6. The bifidobacterium culture equipment of claim 5, further comprising a liquid collector, wherein the liquid collector comprises a liquid collecting seat, a liquid collecting rotary column, a liquid collecting rotary table, a liquid collecting pipe, a liquid collecting opening and a liquid collecting push rod, the liquid collecting rotary table is rotationally connected with the liquid collecting seat through the liquid collecting rotary column, a plurality of liquid collecting pipes uniformly distributed in a ring shape are fixedly connected on the liquid collecting rotary table, the liquid collecting opening is fixedly connected at the top of the liquid collecting pipe, and the liquid collecting push rod is fixedly connected at the middle part of the liquid collecting opening.
7. The bifidobacterium culture equipment according to claim 1, further comprising temperature adjusting chambers symmetrically arranged on two sides of the culture rotating column, wherein temperature adjusting frames are fixedly connected on two sides of the inner wall of the temperature adjusting chambers, detachable temperature adjusting pipes are arranged on the temperature adjusting frames, and air discharging blocks are fixedly connected on the top of the temperature adjusting chambers.
8. The bifidobacterium culture equipment according to claim 1, further comprising a base and a movable block, wherein the base is of a cross plate-shaped structure, the bottom of the culture turntable is rotationally connected with the middle part of the base, the movable block is symmetrically arranged at the top of the base, and the liquid adding device, the temperature adjusting bin and the liquid collector are fixedly connected at the top of the movable block;
the movable block comprises a movable slide rail, a movable slide block, a movable seat, a movable screw and a movable motor, wherein the movable slide rail is symmetrically arranged at the top of the base, the movable seat is in sliding connection with the movable slide block through the movable slide block, the movable motor is fixedly connected to one end of the base, which is far away from the culture turntable, the movable screw is rotationally connected with the movable motor, and the movable screw is in threaded transmission connection with the movable seat.
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