CN116139949A

CN116139949A - Multichannel microfluidic chip and detection method

Info

Publication number: CN116139949A
Application number: CN202211673443.8A
Authority: CN
Inventors: 林志铿; 苏春阳; 刘峰
Original assignee: Xiamen Wiz Biotech Co ltd
Current assignee: Xiamen Wiz Biotech Co ltd
Priority date: 2023-04-12
Filing date: 2023-04-12
Publication date: 2023-05-23

Abstract

The utility model relates to a multichannel micro-fluidic chip and detection method relates to immunodetection's technical field, it includes body and syringe, this internal at least one reaction channel that is provided with, each reaction channel all has linked together freeze-dried reagent reaction zone and antigen antibody reaction zone in proper order, the upper surface of body still is provided with sample import and inlet port, the installation passageway has in one side of body, the lower extreme of installation passageway and sample import, the lower extreme and the reaction channel of inlet port all communicate with each other, the mobilizable setting of syringe is in the installation passageway, sealing connection between the outer wall of syringe and the inner wall of installation passageway, be provided with the through-hole on the lateral wall of syringe. The injector is convenient to accurately inject the detection sample into the reaction channel.

Description

Multichannel microfluidic chip and detection method

Technical Field

The application relates to the technical field of immunodetection, in particular to a multichannel microfluidic chip and a detection method.

Background

The microfluidic chip technology integrates basic operation units of sample preparation, reaction, separation, detection and the like in biological, chemical and medical analysis processes onto a micron-scale chip, and automatically completes the whole analysis process. Because of its great potential in biological, chemical, medical and other fields, it has been developed into a new research field where the disciplines of biology, chemistry, medicine, fluids, electronics, materials, machinery and the like are crossed.

In the related art, the microfluidic chip mainly comprises a body, a plurality of reaction channels are arranged on the body, two reaction areas are sequentially arranged on each reaction channel, freeze-drying reagents and antigen antibodies are respectively arranged in the two reaction areas, when the microfluidic chip is used, a detection sample is required to be extracted from a test tube by using an injector and then sequentially injected into each reaction channel, the detection sample can flow in the reaction channels and sequentially combine with the freeze-drying reagents and the antigen antibodies in the reaction channels, and finally, the LAMP or other types of detectors are used for scanning the combination to finish detection.

For the above related art, the applicant found that the detection sample is pushed into the reaction channel mainly by using the pushing force of the syringe, because the inner diameter of the reaction channel is smaller, in this process, it is necessary to ensure that the liquid outlet end of the syringe is accurately communicated with the inlet of the reaction channel, and also it is necessary to ensure that the connection tightness between the liquid outlet end of the syringe and the reaction channel is good, so that the detection sample can be injected into the reaction channel, however, the overall volume of the microfluidic chip is relatively smaller, resulting in a greater difficulty in the operation of the above steps, which needs to be improved.

Disclosure of Invention

The application aims to provide a multichannel microfluidic chip and a detection method, which can reduce the operation difficulty of the microfluidic chip.

In a first aspect, the present application provides a multichannel microfluidic chip, which adopts the following technical scheme:

the utility model provides a multichannel micro-fluidic chip, includes the body, have at least one reaction channel in the body, still include first piston spare and second piston spare, be equipped with sample import and inlet port on the body surface respectively, this internal installation channel that sets up along predetermineeing the orientation extension that is equipped with, sample import, inlet port and at least one reaction channel are respectively along installation channel extending direction interval distribution and communicate with each other with the installation channel respectively, first piston spare is located in the installation channel along installation channel extending direction movably, be equipped with in the first piston spare along the accommodation chamber of installation channel extending direction extension, the second piston spare is movably located in the accommodation chamber, between first piston spare and the installation channel respectively airtight connection between second piston spare and the first piston spare, be provided with the through-hole that the intercommunication held the chamber on the lateral wall of first piston spare.

By adopting the technical scheme, when the detection sample is required to be accurately injected into the corresponding detection channel, firstly, the syringe is moved to enable the through hole on the side wall of the syringe to be communicated with the sample inlet, so that the syringe can extract the detection sample positioned in the sample inlet, then, the syringe is moved to enable the through hole on the side wall of the syringe to be sequentially communicated with each channel of reaction channel, when the through hole on the side wall of the syringe is communicated with the reaction channel, the syringe is controlled to inject the detection sample into the reaction channel, only the through hole on the side wall of the syringe is required to be communicated with the reaction channel, so that the detection sample in the syringe can be accurately injected into the reaction channel, after the detection sample is injected into the reaction channel, the syringe is moved again to enable the through hole on the side wall of the syringe to be aligned with the air inlet, so that the syringe can extract air, and finally, the syringe is moved again so that the through hole on the side wall of the syringe can be sequentially communicated with each channel of reaction channel, and when the through hole on the side wall of the syringe is communicated with the reaction channel, the syringe is controlled to inject the air into the reaction channel, the reaction channel is pushed by the syringe, so that the detection sample can be sequentially moved in the reaction channel by utilizing the air to enable the detection sample to enter the corresponding reaction region to be sequentially in the reaction region, and the antibody can be combined with the antigen.

Optionally, the body is provided with waste liquid pond and return liquid passageway respectively, the waste liquid pond communicates with each reaction channel one end that keeps away from the installation passageway respectively, the waste liquid pond passes through return liquid passageway communicates with each other with the installation passageway, the body surface is provided with the first exhaust hole that communicates with each other with the waste liquid pond.

Through adopting above-mentioned technical scheme, after the use of detection sample is accomplished, can utilize like air or other medium continue to promote the detection sample removal in the reaction channel for in the detection sample gets into the waste liquid pond, the detection sample collection destroyed after being convenient for detect the completion, utilize first exhaust hole, make the detection sample get into in the waste liquid pond after, the air in the waste liquid pond can directly be discharged, avoid the atmospheric pressure in the waste liquid pond too big to lead to the detection sample to be difficult to the condition emergence in the waste liquid pond.

Optionally, a cleaning fluid channel and an overflow pool are further arranged in the body, two ends of the cleaning fluid channel are respectively communicated with the mounting channel and the overflow pool, a flexible liquid storage cover communicated with the cleaning fluid channel is further arranged on the body, and a second exhaust hole communicated with the overflow pool is formed in the outer surface of the body.

Through adopting above-mentioned technical scheme, after the detection is accomplished, can puncture flexible stock solution cover to in making the washing liquid circulation in the flexible stock solution cover to the washing liquid passageway, then utilize the syringe extraction washing liquid in order to wash each reaction channel, further avoid remaining in the reaction channel to detect the sample, utilize the overflow pond to make the body can temporarily store unnecessary washing liquid that actual washing needs, ensure the cleaning performance.

Optionally, the surface of body is provided with the holding tank, the bottom of holding tank communicates with each other with the washing liquid passageway, still be provided with at least one protruding thorn in the bottom of holding tank, flexible liquid storage cover places in the holding tank.

Through adopting above-mentioned technical scheme, utilize the holding tank can fix a position the effect of storing flexible stock solution cover, when making the washing liquid in the flexible stock solution cover flow to the holding tank simultaneously, can directly circulate to in the washing liquid passageway, utilize the protruding thorn that sets up in the holding tank for can reach convenient, and puncture the effect of flexible stock solution cover fast through pressing the flexible stock solution cover.

Optionally, the device further comprises a hollow connecting sleeve, the mounting channel is arranged in the connecting sleeve, a plurality of communication channels which are distributed at intervals are arranged in the side wall of the connecting sleeve, and the cleaning fluid channel, the waste liquid pool, the sample inlet and each reaction channel are communicated with the mounting channel respectively through the communication channels of a single channel.

Through adopting above-mentioned technical scheme, the thickness of body is thinner, utilizes the adapter sleeve can ensure on the body can improve sufficient installation space for the syringe, utilizes the intercommunication passageway can realize the intercommunication relation between installation passageway and washing liquid passageway, sample import lower extreme and each reaction channel.

Optionally, the syringe includes first piston spare and second piston spare, one of them end opening of first piston spare and other one end seal set up, one of them end of second piston spare inserts through first piston spare open-ended one end and locates in the first piston spare, the opposite one end that sets up of first piston spare and second piston spare all stretches out outside the installation passageway, the through-hole sets up on the lateral wall of first piston spare.

Through adopting above-mentioned technical scheme, utilize first piston spare and second piston spare can constitute the syringe, through making the one end that first piston spare and second piston spare set up in opposite all stretch out outside the installation passageway, can be convenient for the inspector remove the syringe whole, or drive second piston spare and remove for first piston spare.

Optionally, the device further comprises a sample tube, wherein a liquid outlet is formed in one end of the sample tube, one end of the sample tube can be inserted into the sample inlet, a one-way valve is arranged in the liquid outlet, a protrusion capable of penetrating through the one-way valve is arranged at the bottom of the sample inlet, a perforation communicated with the mounting channel is formed in the protrusion, and the sample inlet is communicated with the mounting channel through the perforation.

Through adopting above-mentioned technical scheme, insert the lower extreme that has the sample test tube of detecting the sample and locate in the sample import to make the arch that is located sample import bottom wear to establish the check valve, can realize detecting the quick supply of sample, and need not the manual work and in utilizing the syringe to shift the sample import with the detection sample in the sample test tube.

Optionally, valve valves are respectively arranged in the parts of the cleaning liquid channel, the sample inlet, the waste liquid pool and the reaction channels, which are respectively communicated with the mounting channels, each valve comprises a rubber ring and a plurality of valves, each valve is arranged on the inner wall of the rubber ring and circumferentially distributed around the axis of the rubber ring, and each valve is used for isolating two sides of the rubber ring.

Through adopting above-mentioned technical scheme, can play the effect of temporarily cutting off washing liquid passageway, sample import, waste liquid pond and each way reaction channel and installation passageway through the valve, avoid first piston spare because the removal route is great and lead to not plugging corresponding washing liquid passageway, sample import, waste liquid pond or each way reaction channel, washing liquid passageway, sample import, waste liquid pond or reaction channel in liquid backward flow.

In a second aspect, the present application provides a detection method for a multichannel microfluidic chip as described above, which adopts the following technical scheme:

a detection method for a multichannel microfluidic chip, comprising the steps of:

s1, moving the injector to enable the through hole to be communicated with the air inlet, controlling the injector to absorb a certain amount of air, then moving the injector to enable the through hole to be communicated with the sample inlet, and controlling the injector to absorb a sufficient amount of detection sample;

s2, moving the injector to enable the through holes to be communicated with the reaction channels in sequence, pushing quantitative detection samples into the corresponding reaction channels, after the quantitative detection samples are completed, moving the injector to enable the through holes to be directly communicated with the waste liquid pool, and pushing residual detection samples in the injector into the waste liquid pool;

s3, moving the injector to enable the through hole to be communicated with the air inlet, controlling the injector to absorb enough air, then moving the injector to enable the through hole to be sequentially communicated with each channel of reaction channel, injecting the air in the injector into each channel of reaction channel, pushing the detection sample into the freeze-drying reagent reaction zone by utilizing the air to dissolve the freeze-drying reagent, and finally entering the antigen-antibody reaction zone to be fully combined with the antigen-antibody;

s4, after the incubation time of the detection samples in each reaction channel is over, moving the injector to enable the through holes to be communicated with the air inlet holes, controlling the injector to absorb enough air, then moving the injector to enable the through holes to be sequentially communicated with each reaction channel, injecting the air in the injector into each reaction channel, and sending the detection samples in the reaction channels into the waste liquid pool by utilizing the air;

s5, extruding the flexible liquid storage cover to enable the cleaning liquid of the flexible liquid storage cover to gradually fill the cleaning liquid channel, moving the injector to enable the through hole to be communicated with the air inlet hole, controlling the injector to absorb sufficient air, enabling the through hole to be communicated with the cleaning liquid channel when the injector is moved, and controlling the injector to absorb sufficient cleaning liquid;

s6, moving the injector to enable the through holes to be communicated with the reaction channels in sequence, controlling the injector to push quantitative cleaning liquid into the corresponding reaction channels so as to completely push detection samples in the reaction channels into the waste liquid pool, and discharging the injector;

s7, moving the injector to enable the through hole to be communicated with the air inlet hole, controlling the injector to absorb enough air, then moving the injector to enable the through hole to be sequentially communicated with each reaction channel, injecting air in the injector into each reaction channel, and pushing residual cleaning liquid in the reaction channels into the waste liquid pool by utilizing the air.

In summary, the present application includes at least one of the following beneficial technical effects:

1. the injection of the detection sample only keeps the reciprocating control, is easier to realize for the instrument, is integrally designed, simplifies the production, and indirectly provides the injection molding precision of the detection sample;

2. the core body of the first piston member is the cavity body of the second piston member, so that the integrated injection molding production is facilitated, the structure of the chip is simplified, and the injector is integrated on the chip and is a disposable product, so that the pollution of a sample in the chip to an instrument is avoided;

3. the supply of the detection sample is not transferred to the chip by a syringe, so that the possibility of pollution to the detection sample is further reduced, the detection precision of the detection sample is effectively ensured, and the detection sample is more convenient to use;

4. the detected sample after detection can be concentrated on the chip in time, so that the detected sample is convenient to be singly destroyed by being discharged in the later period, and the detection activity is safer.

Drawings

Fig. 1 is a schematic diagram of the upper surface structure of a multichannel microfluidic chip according to embodiment 1 of the present application;

fig. 2 is a schematic diagram of the bottom structure of a multichannel microfluidic chip according to embodiment 1 of the present application;

FIG. 3 is a schematic perspective view of the body of embodiment 1 of the present application;

FIG. 4 is a schematic view of the structure of the syringe of embodiment 1 of the present application;

FIG. 5 is an enlarged schematic view of part A of FIG. 1;

FIG. 6 is an enlarged schematic view of part B of FIG. 2;

FIG. 7 is a schematic view showing the structure of a reaction channel of example 1 of the present application;

FIG. 8 is a schematic view showing the arrangement of the first, second and third loops of embodiment 1 of the present application;

FIG. 9 is a schematic representation of the use of a sample tube according to example 2 of the present application;

fig. 10 is a schematic view of the arrangement of the valve in embodiment 3 of the present application.

In the figure, 1, a body; 11. a sample inlet; 12. an air inlet hole; 121. a first collar; 13. a cleaning liquid channel; 131. a first channel; 132. a second channel; 133. an overflow pool; 134. a second exhaust hole; 14. a reaction channel; 141. a lyophilization reagent reaction zone; 142. an antigen-antibody reaction region; 15. connecting sleeves; 151. a mounting channel; 152. a mounting hole; 153. a limiting hole; 154. a communication passage; 16. a receiving groove; 161. a placement area; 162. a concave region; 163. protruding thorns; 164. a through hole; 165. a third collar; 17. a waste liquid pool; 171. a first exhaust hole; 172. a second collar; 18. a protrusion; 181. perforating; 19. a valve; 191. a rubber ring; 192. a valve; 2. a syringe; 21. a first piston member; 211. a through hole; 22. a second piston member; 3. a sample tube; 31. a liquid outlet; 32. a one-way valve.

Detailed Description

The present application is described in further detail below with reference to fig. 1-10.

Example 1:

referring to fig. 1 and 2, a multichannel microfluidic chip includes a body 1 and a syringe 2, the syringe 2 is movably mounted on one side of the body 1, a cleaning liquid channel 13, an air inlet hole 12, a sample inlet 11 and a plurality of reaction channels 14 (three channels are illustrated) are provided on the body 1, through holes 211 (not illustrated) are provided on a sidewall of the syringe 2, and the through holes 211 on the sidewall of the syringe 2 can be communicated with the cleaning liquid channel 13, the air inlet hole 12, the sample inlet 11 or the reaction channels 14 by moving the syringe 2; wherein, the body 1 comprises a main body and a cover plate (not shown in the figure), the cleaning liquid channel 13 and each reaction channel 14 are arranged on the bottom surface of the main body, and the cover plate is covered on the bottom surface of the main body.

Referring to fig. 2 and 3, a connecting sleeve 15 is disposed on one side of a body 1, the cross section of the connecting sleeve 15 is in a circular shape, the radius of the connecting sleeve 15 is larger than the thickness of the body 1, a mounting channel 151 coaxial with the connecting sleeve 15 is disposed in the connecting sleeve 15, the mounting channel 151 is obtained after expanding from one side of the body 1, the illustrated mounting channel 151 is a circular channel, the radius of which is larger than the thickness of the body 1, and in other embodiments, the mounting channel 151 can be similar to a circle, an ellipse or other shapes; the mounting channel 151 comprises a mounting hole 152 and a limiting hole 153 which are communicated with each other and coaxially arranged, the inner diameter of the mounting hole 152 is larger than that of the limiting hole 153, a plurality of communication channels 154 which are arranged along the axial direction of the connecting sleeve 15 are arranged in the side wall of the connecting sleeve 15, the upper end of each communication channel 154 is communicated with the mounting hole 152, and the lower end of each communication channel 154 is communicated with the bottom surface of the main body; wherein the washing liquid channel, the lower end of the sample inlet 11 and each of the reaction channels 14 are communicated with each other through each of the communication channels 154 to communicate with the mounting hole 152, respectively.

Referring to fig. 3 and 4, the syringe 2 includes a first piston member 21 and a second piston member 22, one end of the first piston member 21 is opened and the other end is closed, one end of the second piston member 22 is inserted into the first piston member 21 through the opened end of the first piston member 21, and a through hole 211 is provided on a side wall of the end of the first piston member 21 remote from the second piston member 22; when the injector 2 is mounted on the body 1, one end of the first piston member 21 with an opening is inserted into the mounting hole 152, and the other end of the first piston member 21 passes through the limiting hole 153 and out of the connecting sleeve 15; the end of the second piston member 22 extending beyond the first piston member 21 passes through the mounting hole 152.

Referring to fig. 5 and 6, a receiving groove 16 is provided on the upper surface of the body 1, the receiving groove 16 includes a placement area 161 and a concave area 162, the upper side of the placement area 161 is communicated with the upper surface of the body 1, the concave area 162 is concavely provided on the bottom of the placement area 161, a through hole 164 communicated with the cleaning liquid channel 13 is provided at the lowest position of the concave area 162, and at least one protruding thorn 163 circumferentially and equidistantly arranged around the axis of the through hole 164 is also provided in the concave area 162; wherein, a flexible liquid storage cover (not shown in the figure) made of plastic film is placed in the accommodating groove 16, the cleaning liquid is filled in the flexible liquid storage cover, when the flexible liquid storage cover is placed in the accommodating groove 16, the bottom edge of the flexible liquid storage cover is contacted with the bottom surface of the placing area 161, and a gap is arranged between the bottom of the thin bag and the protruding thorn 163.

Referring to fig. 5 and 6, an overflow tank 133 and a second vent hole 134 are further provided on the body 1, the overflow tank 133 is provided in the lower side of the body 1 and located at the lower surface of the body, and the second vent hole 134 communicates with both sides of the body; the cleaning solution channel 13 includes a first channel 131 and a second channel 132, two ends of the first channel 131 are respectively communicated with the through hole 164 and the corresponding communication channel 154, two ends of the second channel 132 are respectively communicated with the overflow pool 133 and the middle part of the first channel 131, and one end of the overflow pool 133 away from the second channel 132 is communicated with the lower end of the second vent hole 134.

Referring to fig. 7, at least one reaction region is further provided on the bottom surface of the main body, wherein each reaction region includes a lyophilized reagent reaction region 141 and an antigen-antibody reaction region 142, which are arranged along the width direction of the main body, each reaction channel 14 is respectively matched with each reaction region, and each reaction channel 14 sequentially passes through the corresponding lyophilized reagent reaction region 141 and antigen-antibody reaction region 142; wherein the portions of each reaction channel 14 between the lyophilized reagent reaction region 141 and the antigen-antibody reaction region 142 are arranged in a continuous "S" shape.

Referring to fig. 7, a waste liquid pool 17 is further provided on the bottom surface of the main body, the waste liquid pool 17 is located on a side of the main body away from the connecting sleeve 15, one end of each reaction channel 14 away from the mounting channel 151 is communicated with the waste liquid pool 17, the waste liquid pool 17 is further directly communicated with one of the communication channels 154, and a first exhaust hole 171 is further provided on the main body 1, both ends of which are respectively communicated with the upper surface of the main body 1 and the waste liquid pool 17.

Referring to fig. 8, a first collar 121, a second collar 172, and a third collar 165 are further provided on the upper surface of the body 1, the first collar 121 is coaxially disposed with the sample inlet, the second collar 172 is disposed in communication with the first vent hole 171, and the third collar 165 is disposed in communication with the through hole 164.

The implementation principle of the embodiment of the application is as follows:

when in use, a sample is firstly placed in the sample inlet 11, at this time, the syringe 2 is moved to enable the syringe 2 to extract the detection sample, and then the syringe with the detection sample extracted is moved to a position, so that the syringe 2 can inject the detection sample into the designated reaction channel 14, thereby realizing rapid and accurate injection of the detection sample.

After the detection sample is injected into the reaction channel 14, the cooperation between the injector 2 and the air inlet 12 is utilized again, so that air can be extracted from the inside of the injector 2, and then the injector 2 is moved again, so that the injector 2 can sequentially inject air into each reaction channel 14, the effect that the detection sample sequentially passes through the freeze-drying reagent reaction zone 141 and the antigen-antibody reaction zone 142 by air propulsion is achieved, and the combination of the detection sample, the freeze-drying reagent and the antigen-antibody is realized.

After the combination of the detection samples is completed, the injector 2 is used for extracting the cleaning liquid, and then the injector 2 is moved again, so that the injector 2 can sequentially inject the cleaning liquid into each reaction channel 14, the work of cleaning the reaction channels 14 is realized, and the residual detection samples in the reaction channels 14 are intensively discharged into the waste liquid pool 17, so that the detection samples are conveniently collected and destroyed intensively.

Example 2:

referring to fig. 9, the difference between the embodiment of the present application and embodiment 1 is that the embodiment of the present application further includes a sample tube 3, the upper end of the sample tube 3 is opened, a liquid outlet 31 is provided at one end of the sample tube 3, a check valve 32 for preventing the detection sample in the sample tube 3 from flowing out is provided in the liquid outlet 31, and one end of the sample tube 3 can be installed in the sample inlet 11 by inserting; wherein, a bulge 18 which can pass through the one-way valve 32 is arranged at the bottom of the sample inlet 11, the bulge 18 is arranged on the surface of the cover body, a through hole 181 is arranged in the bulge 18, the lower end of the through hole 181 is communicated with the side wall of the bulge 18, and when the cover plate is arranged on the ground of the main body, the lower end of the through hole 181 is communicated with a corresponding communication channel 154.

When the detection sample needs to be supplied to the multichannel microfluidic chip, the lower end of the sample tube 3 containing the detection sample is only required to be inserted into the sample inlet 11, the protrusion 18 in the bottom of the sample inlet 11 penetrates through the one-way valve 32, and at this time, the detection sample in the sample tube 3 can be discharged through the through hole 181 in the protrusion 18.

Example 3:

referring to fig. 10, in the embodiment of the present application, a difference between the embodiment and embodiment 1 is that in the embodiment of the present application, valve valves 19 are disposed in the positions where each of the communication channels 154 is respectively communicated with the mounting channel 151, each valve 19 includes a rubber ring 191 and a valve 192, the valve 192 is circumferentially arranged around the axis of the rubber ring 191 and provided with a plurality of pieces, and when each valve 192 is not subjected to an external force, each valve 192 is closely contacted to achieve the effect of isolating two sides of the rubber ring 191.

When the syringe 2 draws the corresponding or discharged liquid, the valves 192 are bent due to the negative pressure, thereby achieving the effect of conducting the two sides of the rubber ring 191, so that the liquid in the chip body 1 can circulate.

The application also discloses a detection method for the multichannel microfluidic chip, which comprises the following steps: s1, moving the injector 2 to enable the through hole 211 to be communicated with the air inlet hole 12, controlling the injector 2 to absorb a certain amount of air, then moving the injector 2 to enable the through hole 211 to be communicated with the sample inlet, and controlling the injector 2 to absorb a sufficient amount of detection sample;

s2, moving the injector 2 to enable the through holes 211 to be communicated with the reaction channels 14 in sequence, pushing quantitative detection samples into the corresponding reaction channels, after the quantitative detection samples are completed, moving the injector 2 to enable the through holes 211 to be directly communicated with the waste liquid pool 17, and pushing residual detection samples in the injector 2 into the waste liquid pool 17;

s3, moving the injector 2 to enable the through holes 211 to be communicated with the air inlet holes 12, controlling the injector 2 to suck enough air, then moving the injector 2 to enable the through holes 211 to be sequentially communicated with the reaction channels 14, injecting the air in the injector 2 into the reaction channels 14, pushing a detection sample into the freeze-drying reagent reaction area 141 by using the air to dissolve the freeze-drying reagent, and finally entering the antigen-antibody reaction area 142 to be fully combined with the antigen-antibody;

s4, after the incubation time of the detection samples in each reaction channel 14 is over, moving the injector 2 so that the through holes 211 are communicated with the air inlet holes 12, controlling the injector 2 to absorb enough air, then moving the injector 2 so that the through holes 211 are sequentially communicated with each reaction channel 14, injecting the air in the injector 2 into each reaction channel 14, and sending the detection samples in the reaction channels 14 into the waste liquid pool 17 by using the air;

s5, extruding the flexible liquid storage cover to enable the cleaning liquid of the flexible liquid storage cover to gradually fill the cleaning liquid channel, simultaneously, moving the injector 2 to enable the through holes 211 to be communicated with the air inlet holes 12, controlling the injector 2 to suck sufficient air, and then enabling the through holes 211 to be communicated with the cleaning liquid channel when the injector 2 is moved, and controlling the injector 2 to suck sufficient cleaning liquid;

s6, moving the injector 2 so that the through holes 211 are communicated with the reaction channels 14 in sequence, controlling the injector 2 to push quantitative cleaning liquid into the corresponding reaction channels 14 so as to push detection samples in the reaction channels 14 into the waste liquid pool 17 completely, and discharging the injector 2;

s7, moving the injector 2 to enable the through holes 211 to be communicated with the air inlet holes 12, controlling the injector 2 to suck enough air, then moving the injector 2 to enable the through holes 211 to be sequentially communicated with the reaction channels 14, injecting the air in the injector 2 into the reaction channels 14, and pushing the residual cleaning liquid in the reaction channels 14 into the waste liquid pool 17 by using the air.

The embodiments of this embodiment are all preferred embodiments of the present application, and are not intended to limit the scope of the present application, in which like parts are denoted by like reference numerals. Therefore: all equivalent changes in structure, shape and principle of this application should be covered in the protection scope of this application.

Claims

1. The utility model provides a multichannel micro-fluidic chip, includes body (1), have at least one reaction channel (14) in body (1), its characterized in that still includes first piston (21) and second piston (22), be equipped with sample import (11) and inlet port (12) on body (1) surface respectively, be equipped with in body (1) along predetermineeing installation channel (151) that the orientation extends to set up, sample import (11), inlet port (12) and at least one reaction channel (14) are respectively along installation channel (151) extending direction interval distribution and respectively with installation channel (151) communicate with each other, first piston (21) are located in installation channel (151) along installation channel (151) extending direction movably, be equipped with in first piston (21) along the accommodation chamber that installation channel (151) extending direction extends, second piston (22) are movably located in the accommodation chamber, between first piston (21) and installation channel (151) second piston (22) and first piston (21) are connected with each other and are connected with airtight chamber (211) respectively, first piston (21) are provided with the through-hole.

2. The multi-channel microfluidic chip according to claim 1, wherein a waste liquid pool (17) and a liquid return channel are respectively arranged in the body (1), the waste liquid pool (17) is respectively communicated with one end of each reaction channel (14) far away from the mounting channel (151), the waste liquid pool (17) is communicated with the mounting channel (151) through the liquid return channel, and a first exhaust hole (171) communicated with the waste liquid pool (17) is arranged on the outer surface of the body (1).

3. The multi-channel microfluidic chip according to claim 2, wherein the body (1) is further provided with a cleaning solution channel (13) and an overflow tank (133) respectively, two ends of the cleaning solution channel (13) are respectively communicated with the mounting channel (151) and the overflow tank (133), the body (1) is further provided with a flexible liquid storage cover communicated with the cleaning solution channel (13), and the outer surface of the body (1) is provided with a second exhaust hole (134) communicated with the overflow tank (133).

4. A multi-channel microfluidic chip according to claim 3, wherein the outer surface of the body (1) is provided with a receiving groove (16), the bottom of the receiving groove (16) is communicated with the cleaning solution channel (13), at least one protruding thorn (163) is further provided in the bottom of the receiving groove (16), and the flexible liquid storage cover is placed in the receiving groove (16).

5. A multichannel microfluidic chip according to claim 3, further comprising a hollow connecting sleeve (15), wherein the mounting channel (151) is arranged in the connecting sleeve (15), a plurality of communication channels (154) which are arranged at intervals are arranged in the side wall of the connecting sleeve (15), and the cleaning fluid channel (13), the waste liquid pool (17), the sample inlet (11) and each reaction channel (14) are respectively communicated with the mounting channel (151) through the single communication channel (154) in sequence.

6. The multichannel microfluidic chip according to claim 1, further comprising a sample tube (3), wherein one end of the sample tube (3) is provided with a liquid outlet (31), one end of the sample tube (3) can be inserted into the sample inlet (11), a one-way valve (32) is arranged in the liquid outlet (31), a protrusion (18) capable of penetrating the one-way valve (32) is arranged at the bottom of the sample inlet (11), a perforation (181) communicated with the mounting channel (151) is arranged in the protrusion (18), and the sample inlet (11) is communicated with the mounting channel (151) through the perforation (181).

7. A multi-channel microfluidic chip according to claim 3, wherein valve valves (19) are arranged in the parts of the cleaning liquid channel (13), the sample inlet (11), the waste liquid tank (17) and the reaction channels (14) which are respectively communicated with the mounting channels (151), each valve (19) comprises a rubber ring (191) and a plurality of valves (192), each valve (192) is arranged on the inner wall of the rubber ring (191) and circumferentially distributed around the axis of the rubber ring (191), and each valve (192) is used for isolating two sides of the rubber ring (191).

8. A detection method for a multichannel microfluidic chip according to any of claims 1-8, characterized by comprising the steps of:

s1, moving the injector (2) to enable the through hole (211) to be communicated with the air inlet hole (12), controlling the injector (2) to suck a certain amount of air, then moving the injector (2) to enable the through hole (211) to be communicated with the sample inlet (11), and controlling the injector (2) to suck a sufficient amount of detection sample;

s2, moving the injector (2) to enable the through holes (211) to be communicated with the reaction channels (14) in sequence, pushing quantitative detection samples into the corresponding reaction channels (14), after the quantitative detection samples are completed, moving the injector (2) to enable the through holes (211) to be directly communicated with the waste liquid pool (17), and pushing residual detection samples in the injector (2) into the waste liquid pool (17);

s3, moving the injector (2) to enable the through holes (211) to be communicated with the air inlet holes (12), controlling the injector (2) to suck enough air, then moving the injector (2) to enable the through holes (211) to be sequentially communicated with the reaction channels (14), injecting the air in the injector (2) into the reaction channels (14), pushing a detection sample into the freeze-drying reagent reaction area (141) by utilizing the air to dissolve the freeze-drying reagent, and finally entering the antigen-antibody reaction area (142) to be fully combined with antigen-antibody;

s4, after the incubation time of the detection samples in each reaction channel (14) is over, moving the injector (2) to enable the through holes (211) to be communicated with the air inlet holes (12), controlling the injector (2) to absorb enough air, then moving the injector (2) to enable the through holes (211) to be communicated with each reaction channel (14) in sequence, injecting the air in the injector (2) into each reaction channel (14), and conveying the detection samples in the reaction channels (14) into the waste liquid pool (17) by utilizing the air;

s5, extruding the flexible liquid storage cover to enable the cleaning liquid of the flexible liquid storage cover to gradually fill the cleaning liquid channel, simultaneously, moving the injector (2) to enable the through holes (211) to be communicated with the air inlet holes (12), controlling the injector (2) to suck enough air, and then enabling the through holes (211) to be communicated with the cleaning liquid channel (13) when the injector (2) is moved, and controlling the injector (2) to suck enough cleaning liquid;

s6, moving the injector (2) to enable the through holes (211) to be communicated with the reaction channels (14) in sequence, controlling the injector (2) to push quantitative cleaning liquid into the corresponding reaction channels (14) so as to push detection samples in the reaction channels (14) into the waste liquid pool (17) completely, and discharging the injector (2);

s7, moving the injector (2) to enable the through holes (211) to be communicated with the air inlet holes (12), controlling the injector (2) to suck enough air, then moving the injector (2) to enable the through holes (211) to be sequentially communicated with the reaction channels (14), injecting the air in the injector (2) into the reaction channels (14), and pushing the residual cleaning liquid in the reaction channels (14) into the waste liquid pool (17) by utilizing the air.