CN116134150A - 病原体特异性核酸片段及其应用 - Google Patents

病原体特异性核酸片段及其应用 Download PDF

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CN116134150A
CN116134150A CN202180063353.4A CN202180063353A CN116134150A CN 116134150 A CN116134150 A CN 116134150A CN 202180063353 A CN202180063353 A CN 202180063353A CN 116134150 A CN116134150 A CN 116134150A
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seq
pathogen
nucleic acid
specific nucleic
amplification
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陈奇涵
王强
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Nanjing Maixike Biotechnology Co ltd
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Nanjing Maixike Biotechnology Co ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/10—Enterobacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/14—Streptococcus; Staphylococcus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Toxicology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

提供了多种病原体特异性核酸片段。还提供了鉴定一种或多种病原体的方法，包括：1)提供可能包括所述病原体的样品；以及2)检测所述样品中是否存在病原体特异性核酸片段或者检测所述样品中病原体特异性核酸片段的含量，其中所述病原体特异性核酸片段是否存在或其含量分别反映与所述病原体特异性核酸片段对应的病原体在所述样品中的是否存在或其含量。提供的病原体特异性核酸片段可用于对病原体进行快速鉴定，在临床上应用时阳性检出率高，检测周期短。

Description

PCT国内申请，说明书已公开。

Claims (22)

  1. PCT国内申请，权利要求书已公开。
CN202180063353.4A 2020-09-16 2021-09-16 病原体特异性核酸片段及其应用 Pending CN116134150A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CN2020115682 2020-09-16
CNPCT/CN2020/115682 2020-09-16
PCT/CN2021/118722 WO2022057854A1 (zh) 2020-09-16 2021-09-16 病原体特异性核酸片段及其应用

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CN116134150A true CN116134150A (zh) 2023-05-16

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WO (1) WO2022057854A1 (zh)

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* Cited by examiner, † Cited by third party
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CN118345184A (zh) * 2023-03-03 2024-07-16 中国医学科学院药用植物研究所 基于时珍法的中药鉴定方法及应用

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US20100076057A1 (en) * 2008-09-23 2010-03-25 Northwestern University TARGET DNA INTERFERENCE WITH crRNA
BRPI0922437A2 (pt) * 2008-12-11 2019-09-24 Bio Arch Lab Inc biossíntese de commodities químicas
FR2942806B1 (fr) * 2009-03-03 2011-09-02 Assist Publ Hopitaux De Paris Procede d'identification de germes en milieu liquide
CN103060301A (zh) * 2011-10-20 2013-04-24 中国科学院微生物研究所 乳酸片球菌的n-乙酰神经氨酸醛缩酶及其基因与应用
CN105899228A (zh) * 2013-10-11 2016-08-24 加利西亚健康服务(Sergas) 减毒活疫苗
WO2016176404A1 (en) * 2015-04-30 2016-11-03 The Brigham And Women's Hospital, Inc. Methods and kits for cloning-free genome editing
TWI649331B (zh) * 2015-06-11 2019-02-01 國立中興大學 鮑氏不動桿菌（Ａｃｉｎｅｔｏｂａｃｔｅｒ ｂａｕｍａｎｎｉｉ）多胜肽抗原及其抗體以及編碼該抗原之核酸
CN110273012B (zh) * 2018-03-13 2022-11-04 厦门大学 一种检测败血症病原体的方法
CN108588246A (zh) * 2018-04-28 2018-09-28 宁波市鄞州人民医院 一种检测下呼吸道细菌特异性基因的引物、探针、方法及试剂盒
CN109811072B (zh) * 2019-02-28 2022-05-06 广州微远医疗器械有限公司 用于结核分枝杆菌复合群的crispr检测引物组及其用途
CN110499323A (zh) * 2019-03-28 2019-11-26 宁夏医科大学 一种鸟苷酸环化酶催化合成环鸟苷二磷酸的方法
CN110029182B (zh) * 2019-04-19 2020-06-23 武汉大学 快速检测葡萄球菌MecA的试剂盒及方法
CN111534643B (zh) * 2020-07-10 2020-10-09 上海科技大学 一种检测呼吸道病原的核酸的试剂盒、检测方法及应用

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