CN116114600A - Application of paper in potato detoxification mini-potato production and rapid propagation and field planting method of tissue culture seedlings for potato detoxification mini-potato production - Google Patents
Application of paper in potato detoxification mini-potato production and rapid propagation and field planting method of tissue culture seedlings for potato detoxification mini-potato production Download PDFInfo
- Publication number
- CN116114600A CN116114600A CN202211556102.2A CN202211556102A CN116114600A CN 116114600 A CN116114600 A CN 116114600A CN 202211556102 A CN202211556102 A CN 202211556102A CN 116114600 A CN116114600 A CN 116114600A
- Authority
- CN
- China
- Prior art keywords
- potato
- culture
- tissue culture
- production
- seedling
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/002—Culture media for tissue culture
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/25—Root crops, e.g. potatoes, yams, beet or wasabi
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
Abstract
The invention belongs to the technical field of plant tissue culture and particularly relates to application of paper in potato virus-free micro-potato production and a rapid propagation and field planting method of tissue culture seedlings for potato virus-free micro-potato production. According to the invention, the paper is used as a carrier in the production of the potato detoxified micro-potato, and can support the floating stem section, so that the floating stem section is prevented from being submerged in a culture medium, the quantity of glass seedlings is reduced, nutrient elements in the culture medium are easier to absorb and longer, boiling is not needed, the culture medium is quickly manufactured, cutting links are reduced, the inoculation speed is improved, and the liquid culture of the potato is realized; after the tissue culture seedlings are bred, one strain of the tissue culture seedlings can be well separated, the tissue culture seedlings which are not good in strain isolation are not required to be cleaned like a solid culture medium, and the production cost is reduced.
Description
Technical Field
The invention belongs to the technical field of plant tissue culture and particularly relates to application of paper in potato virus-free micro-potato production and a rapid propagation and field planting method of tissue culture seedlings for potato virus-free micro-potato production.
Background
The seasonality of potato detoxified mini-potato production is strong, and the yield and quality are directly related. Therefore, a large number of tissue culture seedlings for field planting are required to be produced in a very short field planting suitable period, and thousands of soldiers are required to maintain the arms. The enterprises for large-scale industrialized production of the potato detoxified mini-potatoes face the problem that the tissue culture seedlings are suitable for short planting time, the tissue culture seedlings cannot be prepared for seeds in advance like other crops, the seeds can be produced only one month in advance, operators cannot take one person at will to operate on duty, the operators must be skilled, and if a sufficient number of operators are kept throughout the year, the operators are a huge burden and cost for the enterprises.
Disclosure of Invention
The invention aims to make up the defects of the prior art, improve the growth speed of tissue culture seedlings for producing the potato detoxified mini-potatoes, shorten the seedling stage and reduce the production cost.
The invention provides an application of paper in the production of potato detoxified micro-potatoes.
Preferably, the paper comprises paper towels and/or newspapers.
The invention also provides a culture material for culturing the tissue culture seedling for producing the potato detoxified mini-potato, wherein the culture material comprises a culture medium and a carrier;
the culture medium comprises the following components: KNO (KNO) 3 1900mg/L、NH 4 Cl 1100～1500mg/L、KH 2 PO 4 170mg/L、MgSO 4 ·7H 2 O 370mg/L、Ca(NO 3 ) 2 350～480mg/L、KI 0.83mg/L、H 3 BO 3 6.2mg/L、MnSO 4 ·4H 2 O 22.3mg/L、ZnSO 4 ·7H 2 O 8.6mg/L、Na 2 MoO 4 ·2H 2 O0.25mg/L、CuSO 4 ·5H 2 O 0.025mg/L、CoCl 2 ·6H 2 O 0.025mg/L、Na 2 ·EDTA37.25mg/L、FeSO 4 ·7H 2 27.85mg/L of O, 100mg/L of inositol, 2mg/L of glycine, 0.1mg/L of thiamine hydrochloride, 0.1mg/L of pyridoxine hydrochloride, 0.5mg/L of nicotinic acid, 30g/L of sucrose and the balance of water;
the carrier comprises paper.
The invention also provides application of the culture material in the production of the potato detoxified micro-potato.
The invention also provides a rapid propagation method of the tissue culture seedling for producing the potato detoxified mini-potato based on the culture material of the technical proposal, which comprises the following steps:
cutting the potato parent seedling according to 1-2 leaves as one section to obtain a potato leaf-containing stem section;
placing the carrier and the culture medium into a culture device, inoculating the potato leaf-containing stem segments, inoculating 25-28 stem segments per bottle, and culturing for 15-20 d to obtain the tissue culture seedling for producing the potato detoxicated miniature potato;
the illumination intensity of the culture is 3300Lx, the temperature of the light culture in the culture is 22-24 ℃, and the temperature of the dark culture is 16-18 ℃.
Preferably, in the above-mentioned cultivation, the time of the light cultivation is 9 to 11 hours from the start of cultivation for 0 to 5 days, and the time of the light cultivation is 14 to 18 hours after the cultivation for 5 days.
Preferably, the culture device comprises a 240-type culture flask; inoculating 25-28 potato leaf-containing stem segments into each 240-type culture flask, and using 30mL of culture medium for each 25-28 potato leaf-containing stem segments;
the length of the leaf-containing stem section of the potato is 0.5-1 cm.
The invention also provides a field planting method of the tissue culture seedling for producing the potato detoxified mini-potato, which comprises the following steps:
the tissue culture seedling for producing the potato virus-free miniature potato, which is obtained by the rapid propagation method, is divided into two parts, so that the head of the tissue culture seedling for producing the potato virus-free miniature potato and the tissue culture Miao Weibu for producing the potato virus-free miniature potato are respectively cut.
Preferably, before the cutting, the method further comprises the step of soaking the head of the tissue culture seedling for producing the potato detoxified mini-potato and the tissue culture Miao Weibu for producing the potato detoxified mini-potato by using a plant growth regulator.
Preferably, the plant growth regulator comprises gibberellin, indolebutyric acid, naphthylacetic acid and brassicin; the concentration of gibberellin in the plant growth regulator is 1.5ppm, the concentration of indolebutyric acid is 5ppm, the concentration of naphthalene acetic acid is 5ppm, and the concentration of brassin is 0.04ppm;
the soaking time is 5min.
The invention provides an application of paper in the production of potato detoxified micro-potatoes. According to the invention, paper is used as a carrier in the production of the potato detoxification micro-potato, and can replace gel such as carrageenan or agar, so that the boiling time of tissue culture seedlings for the production of the potato detoxification micro-potato in the conventional culture process is reduced, a culture medium is quickly manufactured, the cutting links in the inoculation process are reduced, the inoculation speed is improved, the liquid culture of the potato is realized, and nutrient elements in the culture medium are easier to absorb and the stem length is quick; and the paper can support the floating stem segment, avoid being submerged in the culture medium, and reduce the quantity of glass seedlings. After the tissue culture seedlings are grown, the tissue culture seedlings which are grown do not need to be cleaned like a solid culture medium and are not difficult to separate like a culture medium with a leather surface or the like. The invention can well separate one strain of tissue culture seedling and reduce the production cost.
According to the invention, by adjusting the formula of the culture medium, ammonium nitrate in the MS culture medium is replaced by ammonium chloride, calcium chloride is replaced by calcium nitrate, and the modified MS culture medium is used as the culture medium for producing the potato detoxified micro-potato, so that the explosion risk in the culture process can be reduced, and simultaneously, the nutrient elements required by the tissue culture of the potato are satisfied, thereby realizing the production of the potato detoxified micro-potato.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are required to be used in the embodiments will be briefly described below.
FIG. 1 is a graph of results of a solid medium look-ahead;
FIG. 2 is a 240-type flask containing potato leaf-containing stem segments, medium and napkin in step (4) of example 3;
FIG. 3 shows the results of the cultivation of example 3 after inoculation of potato leaf-containing stem segments and cultivation for 15 d;
FIG. 4 is a cut tissue culture seedling head and tissue culture Miao Weibu of example 4;
FIG. 5 is a photograph of a head of a field planting tissue culture seedling of example 4;
FIG. 6 is a photograph of the tail of the field planting tissue culture seedling of example 4;
FIG. 7 is a photograph of example 4 after one week of field planting the tissue culture seedling head;
FIG. 8 is a photograph of example 4 after one week of field tissue culture Miao Weibu;
FIG. 9 is a photograph of example 4 after one month of field planting of the head and tail of the tissue culture seedlings.
Detailed Description
The invention provides application of paper in potato detoxification mini-potato production.
In the present invention, the paper preferably comprises paper towels and/or newspapers. The invention has no strict requirements on the thickness and quality of the paper, and can be obtained conventionally, such as waste newspapers or waste tissues. The variety of potatoes of the present invention is preferably one or more of atlantic, netherlands, ewing 885, middling, and 226. According to the invention, paper is used as a carrier in the production of the potato detoxification micro-potato, especially as a carrier of the tissue culture seedling for the production of the potato detoxification micro-potato, so that the material of the tissue culture seedling for the production of the potato detoxification micro-potato can be supported and floated to be cultured, the submerging in a culture medium is avoided, the quantity of glass seedlings is reduced, the sprouting speed is improved, the nutrient elements in the culture medium are efficiently absorbed, the growth speed of the culture material is promoted, and the liquid culture of the tissue culture seedling for the production of the potato detoxification micro-potato is realized; after the tissue culture seedlings are bred, one strain of tissue culture seedlings can be well separated, cleaning is not needed like a solid culture medium, and the tissue culture seedlings bred like a liquid culture medium with lint are not needed to be separated; and the paper can replace gel such as carrageenan or agar, so that the high-temperature boiling process at 100 ℃ is avoided, the culture medium is quickly manufactured, cutting is not needed, and the inoculation speed is improved. In the specific implementation process, the invention discovers that when the solid culture medium is used for culturing the tissue culture seedlings, the explants (stem segments) are required to be subjected to cuttage one by one (as shown in figure 1), the number of inoculated bottles per day is 200 bottles, and by using the scheme of the invention, the liquid culture of the tissue culture seedlings is realized by taking paper as a carrier, the number of inoculated bottles per day is 330 bottles, so that the culture efficiency is greatly improved, and the production cost is reduced.
The invention also provides a culture material for culturing the tissue culture seedling for producing the potato detoxified mini-potato, wherein the culture material comprises a culture medium and a carrier;
the culture medium comprises the following components: KNO (KNO) 3 1900mg/L、NH 4 Cl 1100～1500mg/L、KH 2 PO 4 170mg/L、MgSO 4 ·7H 2 O 370mg/L、Ca(NO 3 ) 2 350～480mg/L、KI 0.83mg/L、H 3 BO 3 6.2mg/L、MnSO 4 ·4H 2 O 22.3mg/L、ZnSO 4 ·7H 2 O 8.6mg/L、Na 2 MoO 4 ·2H 2 O0.25mg/L、CuSO 4 ·5H 2 O 0.025mg/L、CoCl 2 ·6H 2 O 0.025mg/L、Na 2 ·EDTA37.25mg/L、FeSO 4 ·7H 2 27.85mg/L of O, 100mg/L of inositol, 2mg/L of glycine, 0.1mg/L of thiamine hydrochloride, 0.1mg/L of pyridoxine hydrochloride, 0.5mg/L of nicotinic acid, 30g/L of sucrose and the balance of water;
the carrier comprises paper.
In the present invention, the paper preferably comprises paper towels and/or newspapers, and further preferably comprises inexpensive low grade paper towels, such as toilet paper. The invention has no strict requirements on the source of the paper, and is conventionally obtained. The invention uses paper as raw material for producing potato detoxified mini-potato, which can reduce production cost.
In the present invention, NH in the medium 4 The Cl concentration is preferably 1100-1300 mg/L. The invention uses NH 4 Cl instead of NH 4 NO 3 The method can reduce the explosion risk in the culture process and simultaneously meet the nutrient elements required by potato tissue culture.
In the present invention, ca (NO 3 ) 2 Preferably, the concentration of (C) is 350mg/L. The invention uses Ca (NO) 3 ) 2 Rather than calcium chloride CaCl 2 The concentration of chloride ions in the culture medium can be reduced, the damage to the potatoes caused by the too high concentration of the chloride ions is avoided, and meanwhile, the nutrient elements required during the tissue culture of the potatoes are met.
According to the invention, the formula of the culture medium is firstly adjusted, on the premise that nutrient elements required by the materials for culturing the tissue culture seedlings for producing the potato detoxified micro-potatoes are met, the explosion hazard is reduced, the concentration of chloride ions in the culture medium is reduced, the damage to the potatoes caused by the excessively high concentration of the chloride ions is avoided, the materials for culturing the potato detoxified micro-potatoes can be supported and floated by using paper, the materials are prevented from being submerged in the culture medium, the quantity of glass seedlings is reduced, and the production efficiency of the tissue culture seedlings for producing the potato detoxified micro-potatoes is improved.
The invention also provides application of the culture material in the production of the potato detoxified micro-potato.
The invention also provides a rapid propagation method of the tissue culture seedling for producing the potato detoxified mini-potato based on the culture material of the technical proposal, which comprises the following steps:
cutting the potato parent seedling according to 1-2 leaves as one section to obtain a potato leaf-containing stem section;
placing the carrier and the culture medium into a culture device, inoculating the potato leaf-containing stem segments, inoculating 25-28 stem segments per bottle, and culturing for 15-20 d to obtain the tissue culture seedling for producing the potato detoxicated miniature potato;
the illumination intensity of the culture is 3300Lx, the temperature of the light culture in the culture is 22-24 ℃, and the temperature of the dark culture is 16-18 ℃.
According to the invention, the potato parent seedling is sheared according to 1-2 leaves as one section, preferably the potato parent seedling is sheared according to 1 leaf as one section, and the potato leaf-containing stem section is obtained. In the invention, the potato parent seedlings are preferably detoxified aseptic seedlings; the length of the potato leaf-containing stem section is preferably 0.5-1 cm. In the specific implementation process, the lower incision of the potato leaf-containing stem section is 0.3-0.5cm away from the leaf axil.
After the potato leaf-containing stem section is obtained, the carrier and the culture medium in the technical scheme are placed into a culture device, then the potato leaf-containing stem section is inoculated, and the culture is carried out for 15-20 days, so that the tissue culture seedling for producing the potato detoxified miniature potato is obtained. In the present invention, the size of the bottom of the culture apparatus is preferably not larger than the size of the carrier, and more preferably is equal to the size of the carrier. The culture device of the invention preferably comprises a 240-type culture flask. In the implementation process of the invention, when a 240-type culture flask is used as a culture device, the paper is preferably a round block with the diameter larger than or equal to the bottom size of the 240-type culture flask. The invention preferably inoculates 25-28 potato leaf-containing stem segments per 240 culture flasks, and uses 30mL of culture medium per 25-28 potato leaf-containing stem segments. The inoculation mode is not strictly required, and the potato leaves and stems are gently scattered and uniformly shaken and then are placed into a culture device containing a carrier and a culture medium. In the specific implementation process, the potato leaves and stems do not need to be cut one by using tweezers, and can be uniformly distributed on paper only by slightly scattering or shaking, and whether the front of the stems faces upwards or not is not needed, so that the inoculation speed is greatly improved.
In the present invention, the temperature of the light culture in the culture is 22 to 24 ℃, preferably 23 ℃; the illumination intensity of the light culture is 3300Lx; the method comprises the steps of carrying out a first treatment on the surface of the The temperature of the dark culture in the above culture is preferably 16 to 18℃and more preferably 17 ℃.
In the present invention, the time of the light culture is preferably 9 to 11 hours, preferably 10 hours, for 0 to 5 days of the culture; after 5d of cultivation, the time of light cultivation is preferably 14 to 18 hours, more preferably 15 to 17 hours, and still more preferably 16 hours. The invention can ensure that the potato leaves and stems contain roots and buds after being cultured for 5 days, save energy and reduce cost.
The rapid propagation method provided by the invention realizes the liquid culture of the tissue culture seedling for producing the potato virus-free miniature potato, and due to the good fluidity of nutrient elements in the liquid culture, the tissue culture seedling grows rapidly by limiting the culture conditions, the seedling stage is shortened by 20-25 days, the tissue culture seedling for producing the potato virus-free miniature potato can be obtained only by 15 days, and the tissue culture seedling is easy to separate after the seedling is formed, and can be used for subsequent field planting treatment without cleaning.
The invention also provides a field planting method of the tissue culture seedling for producing the potato detoxified mini-potato, which comprises the following steps:
the tissue culture seedling for producing the potato virus-free miniature potato, which is obtained by the rapid propagation method, is divided into two parts, so that the head of the tissue culture seedling for producing the potato virus-free miniature potato and the tissue culture Miao Weibu for producing the potato virus-free miniature potato are respectively cut.
In the invention, the tissue culture seedling for producing the potato detoxified micro-potato is preferably divided along the direction perpendicular to the length direction of the tissue culture seedling for producing the potato detoxified micro-potato to obtain the head of the tissue culture seedling for producing the potato detoxified micro-potato and the tissue culture Miao Weibu for producing the potato detoxified micro-potato; the length ratio of the head part of the tissue culture seedling for producing the potato detoxified micro-potato to the tail part of the tissue culture seedling for producing the potato detoxified micro-potato is preferably 1:2. the method for separating the tissue culture seedlings for producing the potato detoxified mini-potatoes, which is obtained by the rapid propagation method, has no strict requirements on the mode of separating the tissue culture seedlings for producing the potato detoxified mini-potatoes, and can be carried out by conventional operation. According to the method, the tissue culture seedlings for producing the potato virus-free micro-potatoes, which are obtained by the rapid propagation method, are divided into two parts, namely the head part of the tissue culture seedlings for producing the potato virus-free micro-potatoes and the tissue culture Miao Weibu for producing the potato virus-free micro-potatoes, and the two parts are planted one by one, so that the number of the tissue culture seedlings for field planting is increased by 2/3-1 time, seasonal field planting seedlings in the production process of the potato virus-free micro-potatoes are rapidly met, a large-area production seedling is completed by using fewer inoculating personnel, the production cost is reduced, and the production efficiency of the potato virus-free micro-potatoes is improved.
After the tissue culture seedling head for producing the potato detoxified micro-potato and the tissue culture Miao Weibu for producing the potato detoxified micro-potato are obtained, the method also preferably comprises the step of soaking the tissue culture seedling head for producing the potato detoxified micro-potato and the tissue culture Miao Weibu for producing the potato detoxified micro-potato by using a plant growth regulator before cutting. In the present invention, the plant growth regulator preferably includes gibberellin, indolebutyric acid, naphthylacetic acid and brassin; the concentration of gibberellin in the plant growth regulator is preferably 1.5ppm; the concentration of the indolebutyric acid in the plant growth regulator is preferably 5ppm; the concentration of naphthalene acetic acid in the plant growth regulator is preferably 5ppm; the concentration of brassicin in the plant growth regulator is preferably 0.04ppm. The invention utilizes the specific plant growth regulator to treat the cutting material, can shorten the seedling time, and reduces the seedling time by at least 2 days compared with the potato tissue culture seedling which is directly planted without soaking.
For further explanation of the present invention, the application of the paper provided by the present invention in potato virus-free micro-potato production and the method for rapid propagation and planting of tissue culture seedlings for potato virus-free micro-potato production will be described in detail with reference to the accompanying drawings and examples, but they should not be construed as limiting the scope of the present invention.
In the specific implementation process, if no special description exists in all culture processes, sterilizing the culture material for 20min at 121 ℃, wherein the culture condition temperature is 24+/-2 ℃, the illumination intensity is 2500Lx, and the photoperiod is 14h illumination+10 h darkness; the technical means used in the examples are conventional means well known to those skilled in the art, and the agents used are conventional experimental agents purchased from commercial sources unless specifically stated otherwise.
Example 1
The specific compositions of the culture medium for culturing the tissue culture seedlings for producing the potato detoxified mini-potatoes are shown in the table 1.
TABLE 1 composition of culture medium for culturing tissue culture seedlings for producing detoxified mini-potatoes
Example 2
The difference from example 1 is that the concentration of calcium nitrate is 350mg/L.
Example 3
The rapid propagation method of the tissue culture seedling for producing the potato detoxified mini-potato comprises the following steps:
(1) Taking a parent seedling of the early large white potato, taking 1-2 leaves as a section, shearing at the position of 0.3-0.5cm below the axillary edge of the leaf, and finally shearing into a potato leaf-containing stem section of 0.5-1 cm;
(2) Cutting low-cost inferior napkin paper into round blocks with the size equal to or slightly smaller than the bottom of a 240-type culture flask, and throwing the round blocks into the 240-type culture flask;
(3) Preparing a culture medium according to the formula of the embodiment 1, stirring uniformly, and directly filling the culture medium into 240 type culture bottles containing low-cost inferior napkins in the step (2), wherein 30mL of culture medium is contained in each 240 type culture bottle;
(4) Putting the potato leaf-containing stem segments obtained by shearing in the step (1) into 240 culture bottles containing low-cost inferior napkins and culture mediums in the step (3), and uniformly shaking or scattering 25-28 potato leaf-containing stem segments per bottle to uniformly distribute the potato leaf-containing stem segments on the low-cost inferior napkins (as shown in figure 2), culturing under the condition of 3300Lx illumination intensity for 10 hours in the first 5 days, changing the illumination period to 16 hours, wherein the illumination temperature is 23+/-1 ℃, and the dark period temperature is 17+/-1 ℃, and culturing for 18 days to obtain the tissue culture seedlings.
Example 4
The field planting method of the tissue culture seedling for producing the potato detoxified mini-potato comprises the following steps:
(1) Taking 100 bottles of 240-type culture bottles for culturing potato stems in example 3, taking out the obtained 2558-strain tissue culture seedlings from the 240-type culture bottles, and cutting one-strain tissue culture seedling into two sections without cleaning to obtain a tissue culture seedling head and a tissue culture Miao Weibu, wherein the length of the tissue culture seedling head is one third of the whole field of the tissue culture seedling, and the result is shown in figure 4 (about obtaining 2057-strain tissue culture seedling heads and 2558-strain tissue culture Miao Weibu);
(2) The head of the tissue culture seedling and the tissue culture Miao Weibu are respectively soaked for 5min by using plant growth regulators; wherein the plant growth regulator consists of gibberellin 1.5ppm, indolebutyric acid 5ppm, naphthylacetic acid 5ppm and brassin 0.04ppm;
(3) After the treatment by using the plant growth regulator is finished, the head of the tissue culture seedling and the tissue culture Miao Weibu are respectively cut, so that the field planting of the tissue culture seedling for producing the potato detoxified mini-potato is realized (fig. 5 and 6);
(4) And (3) after one week of field planting, observing the field planting result (shown in fig. 7 and 8), finding that the survival rate of 2013 plants at the head of the tissue culture seedling is 97.86%, and the survival rate of 2314 plants at Miao Weibu reaches 90.46%. After one month of field planting, the seedling formation was observed (FIG. 9), and the total number of seedlings was 4327, and the seedling formation rate was 169.16%.
The head and the tail of the tissue culture seedling can survive, and the field planting effect is greatly improved.
Comparative example 1
The only difference from example 4 is that no shearing step is performed, the specific steps are as follows:
(1) Taking 100 bottles of 240-type culture bottles for culturing potato stems in example 3, taking out the 2583 strain tissue culture seedlings obtained from the 240-type culture bottles, and soaking for 5min by using a plant growth regulator without cleaning; wherein the plant growth regulator consists of gibberellin 1.5ppm, indolebutyric acid 5ppm, naphthylacetic acid 5ppm and brassin 0.04ppm;
(2) And after the treatment by using the plant growth regulator is finished, cutting the tissue culture seedlings to realize the field planting of the tissue culture seedlings for producing the potato detoxified mini-potatoes.
(3) Planting for one week, finding that the survival rate of the tissue culture seedlings is 2521 strain, and the survival rate is 97.60%; after one month of field planting, the total number of seedlings is 2521, and the seedling rate is 97.6%.
According to the above, the technical scheme provided by the invention can realize rapid propagation and field planting of the tissue culture seedling for producing the potato virus-free micro-potato, improve the field planting effect of the tissue culture seedling for producing the potato virus-free micro-potato and reduce the production cost.
Although the foregoing embodiments have been described in some, but not all, embodiments of the invention, it should be understood that other embodiments may be devised in accordance with the present embodiments without departing from the spirit and scope of the invention.
Claims (10)
1. The application of paper in the production of potato detoxified micro-potato.
2. The use according to claim 1, wherein the paper comprises paper towels and/or newspapers.
3. A culture material for culturing tissue culture seedlings for producing potato detoxified micro-potatoes, which is characterized by comprising a culture medium and a carrier;
the culture medium comprises the following components: KNO (KNO) 3 1900mg/L、NH 4 Cl1100～1500mg/L、KH 2 PO 4 170mg/L、MgSO 4 ·7H 2 O370mg/L、Ca(NO 3 ) 2 350～480mg/L、KI0.83mg/L、H 3 BO 3 6.2mg/L、MnSO 4 ·4H 2 O22.3mg/L、ZnSO 4 ·7H 2 O8.6mg/L、Na 2 MoO 4 ·2H 2 O0.25mg/L、CuSO 4 ·5H 2 O0.025mg/L、CoCl 2 ·6H 2 O0.025mg/L、Na 2 ·EDTA37.25mg/L、FeSO 4 ·7H 2 27.85mg/L of O, 100mg/L of inositol, 2mg/L of glycine, 0.1mg/L of thiamine hydrochloride, 0.1mg/L of pyridoxine hydrochloride, 0.5mg/L of nicotinic acid, 30g/L of sucrose and the balance of water;
the carrier comprises paper.
4. Use of the culture material of claim 3 for the production of detoxified micro-potatoes.
5. A rapid propagation of tissue culture seedlings for potato virus-free micro-potato production based on the culture material of claim 3, comprising the steps of:
cutting the potato parent seedling according to 1-2 leaves as one section to obtain a potato leaf-containing stem section;
placing the carrier and the culture medium into a culture device, inoculating the potato leaf-containing stem segments, inoculating 25-28 stem segments per bottle, and culturing for 15-20 d to obtain the tissue culture seedling for producing the potato detoxicated miniature potato;
the illumination intensity of the culture is 3300Lx, the temperature of the light culture in the culture is 22-24 ℃, and the temperature of the dark culture is 16-18 ℃.
6. The rapid propagation method according to claim 5, wherein in the cultivation, the time of the light cultivation is 9 to 11 hours from the start of cultivation for 0 to 5 days, and the time of the light cultivation is 14 to 18 hours after cultivation for 5 days.
7. The rapid propagation method of claim 5, wherein the culture device comprises a 240-type culture flask; inoculating 25-28 potato leaf-containing stem segments into each 240-type culture flask, and using 30mL of culture medium for each 25-28 potato leaf-containing stem segments;
the length of the leaf-containing stem section of the potato is 0.5-1 cm.
8. The field planting method of the tissue culture seedling for producing the potato detoxified mini-potato is characterized by comprising the following steps:
dividing the tissue culture seedling for producing the potato virus-free miniature potato obtained by the rapid propagation method of any one of claims 5-7 into two parts to obtain the head of the tissue culture seedling for producing the potato virus-free miniature potato and the tissue culture Miao Weibu for producing the potato virus-free miniature potato, and cutting respectively.
9. The method of claim 8, further comprising immersing the potato virus-free mini-potato production tissue culture seedling head and potato virus-free mini-potato production tissue culture Miao Weibu with a plant growth regulator prior to said cutting.
10. The method of claim 9, wherein the plant growth regulator comprises gibberellin, indolebutyric acid, naphthylacetic acid, and brassin; the concentration of gibberellin in the plant growth regulator is 1.5ppm, the concentration of indolebutyric acid is 5ppm, the concentration of naphthalene acetic acid is 5ppm, and the concentration of brassin is 0.04ppm;
the soaking time is 5min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211556102.2A CN116114600A (en) | 2022-12-06 | 2022-12-06 | Application of paper in potato detoxification mini-potato production and rapid propagation and field planting method of tissue culture seedlings for potato detoxification mini-potato production |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211556102.2A CN116114600A (en) | 2022-12-06 | 2022-12-06 | Application of paper in potato detoxification mini-potato production and rapid propagation and field planting method of tissue culture seedlings for potato detoxification mini-potato production |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN116114600A true CN116114600A (en) | 2023-05-16 |
Family
ID=86313734
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211556102.2A Pending CN116114600A (en) | 2022-12-06 | 2022-12-06 | Application of paper in potato detoxification mini-potato production and rapid propagation and field planting method of tissue culture seedlings for potato detoxification mini-potato production |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN116114600A (en) |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104026009A (en) * | 2014-05-23 | 2014-09-10 | 甘肃省农业科学院生物技术研究所 | Purple potato test tube mini-tuber induction method |
| CN104126386A (en) * | 2014-07-14 | 2014-11-05 | 毕节市农业科学研究所 | Potato breeder's seed production method |
| CN104429853A (en) * | 2014-11-14 | 2015-03-25 | 张家港市大新利群农民专业合作社 | Seed potato production method |
| CN105145356A (en) * | 2015-09-10 | 2015-12-16 | 宁波市农业科学研究院 | Rapid propagation method of purple potato minitubers |
| WO2019006470A1 (en) * | 2017-06-30 | 2019-01-03 | Booshoot Llc | Media for rapid and reliable tissue culturing of plants |
| CN109744113A (en) * | 2019-01-30 | 2019-05-14 | 云南洪邦薯业发展有限公司 | A kind of implantation methods of micro potato |
| CN111374050A (en) * | 2018-12-30 | 2020-07-07 | 甘肃康勤薯业有限公司 | Breeding method of test-tube potatoes |
| CN111937745A (en) * | 2020-08-13 | 2020-11-17 | 江苏宝德农业科技有限公司 | Tissue culture method for rapid propagation of tissue culture seedlings for field planting in production process of detoxified miniature potatoes |
-
2022
- 2022-12-06 CN CN202211556102.2A patent/CN116114600A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104026009A (en) * | 2014-05-23 | 2014-09-10 | 甘肃省农业科学院生物技术研究所 | Purple potato test tube mini-tuber induction method |
| CN104126386A (en) * | 2014-07-14 | 2014-11-05 | 毕节市农业科学研究所 | Potato breeder's seed production method |
| CN104429853A (en) * | 2014-11-14 | 2015-03-25 | 张家港市大新利群农民专业合作社 | Seed potato production method |
| CN105145356A (en) * | 2015-09-10 | 2015-12-16 | 宁波市农业科学研究院 | Rapid propagation method of purple potato minitubers |
| WO2019006470A1 (en) * | 2017-06-30 | 2019-01-03 | Booshoot Llc | Media for rapid and reliable tissue culturing of plants |
| CN111374050A (en) * | 2018-12-30 | 2020-07-07 | 甘肃康勤薯业有限公司 | Breeding method of test-tube potatoes |
| CN109744113A (en) * | 2019-01-30 | 2019-05-14 | 云南洪邦薯业发展有限公司 | A kind of implantation methods of micro potato |
| CN111937745A (en) * | 2020-08-13 | 2020-11-17 | 江苏宝德农业科技有限公司 | Tissue culture method for rapid propagation of tissue culture seedlings for field planting in production process of detoxified miniature potatoes |
Non-Patent Citations (1)
| Title |
|---|
| 冯怀章等: ""新疆马铃薯脱毒种薯繁育技术"", 《农村科技》, no. 12, pages 10 - 12 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Ziv et al. | Organs and plantlets regeneration of Gladiolus through tissue culture | |
| Park et al. | Rapid propagation of Phalaenopsis from floral stalk-derived leaves | |
| CN104126511B (en) | The method for tissue culture of a kind of precocious stem of Radix pyri section and culture medium | |
| CN111492973B (en) | Method for obtaining regeneration plants from common camellia oleifera through somatic embryogenesis | |
| CN108770695B (en) | Method for in-vitro tissue culture and rapid seedling establishment of red-heart pitaya | |
| CN111937746A (en) | Series culture kit for regenerating tiger ginger flower plants and application thereof | |
| CN112931198A (en) | Preparation method of pineapple cold-resistant germplasm | |
| KR100533120B1 (en) | Method of plant cell culture from cambium | |
| Bapat et al. | Regulatory factors for in vitro multiplication of sandalwood tree (Santalum album Linn.) I. Shoot bud regeneration and somatic embryogenesis in hypocotyl cultures | |
| CN108770692B (en) | Coconut embryo induction culture medium and method for obtaining in-vitro regeneration plant based on coconut zygotic embryo cell thin-layer culture | |
| CN1709027A (en) | Potato seed breeding method | |
| JP2901021B2 (en) | How to grow bulbs by plant tissue culture | |
| CN109479718A (en) | A kind of rooting method of tissue culture of shinyleaf yellowhorn seedling | |
| CN116114600A (en) | Application of paper in potato detoxification mini-potato production and rapid propagation and field planting method of tissue culture seedlings for potato detoxification mini-potato production | |
| CN110547194A (en) | Tissue culture and rapid propagation method for seeds of bletilla striata | |
| CN112154919B (en) | Culture medium and method for inducing calli of paris polyphylla to directly grow seedlings | |
| CN1224314C (en) | Root inductive method for microbody reproduction of Japan dahurian larch | |
| Samosir et al. | An improved protocol for somatic embryogenesis in coconut (Cocos nucifera L.) | |
| CN109937875B (en) | Rapid propagation method for paphiopedilum high-quality seedling tissue culture through leaf clumpy buds | |
| CN111165356B (en) | Tissue culture propagation method of peony | |
| CN111149703B (en) | Simple, convenient, efficient and high-quality papaya tissue culture seedling rooting method | |
| CN114041421A (en) | Tissue rapid propagation method of avocados | |
| JPS6411250B2 (en) | ||
| CN107173225A (en) | The method that androgenesis is carried out with Sweet Potato Leaf | |
| CN113080059A (en) | Tissue culture propagation method of linalool type sassafras |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |