CN116098144B - Low-temperature storage method, sample storage unit, storage device and transportation device for biological samples - Google Patents
Low-temperature storage method, sample storage unit, storage device and transportation device for biological samples Download PDFInfo
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- CN116098144B CN116098144B CN202310184626.1A CN202310184626A CN116098144B CN 116098144 B CN116098144 B CN 116098144B CN 202310184626 A CN202310184626 A CN 202310184626A CN 116098144 B CN116098144 B CN 116098144B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0278—Physical preservation processes
- A01N1/0284—Temperature processes, i.e. using a designated change in temperature over time
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0242—Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components
- A01N1/0252—Temperature controlling refrigerating apparatus, i.e. devices used to actively control the temperature of a designated internal volume, e.g. refrigerators, freeze-drying apparatus or liquid nitrogen baths
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0263—Non-refrigerated containers specially adapted for transporting or storing living parts whilst preserving, e.g. cool boxes, blood bags or "straws" for cryopreservation
- A01N1/0273—Transport containers
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Abstract
The invention provides a biological sample low-temperature storage method, a sample storage unit, a storage device and a transportation device, wherein the method comprises the following steps: s1, setting a sample storage unit, wherein the sample storage unit comprises a heat insulator made of a high thermal resistance material, and an insulating and heat insulating material is arranged on the periphery of the sample storage unit; s2, in the sample storage unit, a sample bin made of a low-thermal-resistance material is arranged in the heat insulation body, and a sample cavity for placing biological samples is formed in the sample bin; s3, the sample storage unit is arranged between the cold source and the normal-temperature heat source, and the biological sample is placed into the sample cavity for storage. The application of the biological sample low-temperature storage method can effectively design and apply according to the position of the sample bin under the condition of single constant cold source setting, can effectively form a temperature control area with target design temperature in the sample cavity of the biological sample storage method, and meets the biological sample storage application requirements of different storage temperatures.
Description
Technical Field
The invention relates to the technical field of low-temperature storage of biological samples, in particular to a low-temperature storage method of biological samples, a sample storage unit, a storage device and a transportation device.
Background
At present, the long-term low-temperature preservation of biological samples such as cells and tissues for clinical and scientific research mostly uses a traditional freezing method, the biological samples are preserved by liquid nitrogen with the loading temperature of-196 ℃ in a liquid nitrogen tank, the biological samples are stored in liquid nitrogen or gas phases at the upper part of the liquid nitrogen, the biological samples are directly stored in the liquid nitrogen and are sealed by an additional sealing device, otherwise, secondary pollution of the samples is easily caused, the biological samples are stored in the gas phase of the liquid nitrogen, the temperature difference range is large, the storage space conforming to the storage temperature is limited, and the temperature cannot be accurately controlled.
In the prior art, as is known from the disclosure of patent publication CN215123706U, CN204568401U, etc., for storage applications of biological samples, particularly for transportation applications, the biological samples are simply stored in a storage tank of a single cold source, and cannot be effectively applied to biological samples with different biological characteristics. The prior art can not realize the products with the optimal preservation temperature of-130 ℃ to-150 ℃ required by vitrification cryopreservation and accurately control the temperature according to the storage requirements of different samples.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provide a biological sample low-temperature storage method, a sample storage unit, a storage device and a transportation device.
A method for the cryogenic storage of a biological sample comprising the steps of: s1, setting a sample storage unit, wherein the sample storage unit comprises a heat insulator made of a high-thermal-resistance material; arranging an insulating and heat-insulating material on the periphery of the sample storage unit, wherein when one end of the heat insulator is contacted with a cold source and the other end of the heat insulator is contacted with a normal-temperature heat source, the heat insulator has a temperature gradient change condition from the cold source to the normal-temperature heat source; s2, in the sample storage unit, a sample bin made of a low-thermal-resistance material is arranged in the heat insulation body, and a sample cavity for placing biological samples is formed in the sample bin; the sample cavity is provided with an extension size range from the cold source to the normal temperature heat source; when one end of the heat insulator is contacted with a cold source and the other end is contacted with a normal-temperature heat source, the sample cavity forms a temperature control area with stable temperature in the extension size range under the heat conduction effect of a low-thermal-resistance material applied to the sample bin; s3, the sample storage unit is arranged between the cold source and the normal-temperature heat source, and the biological sample is placed into the sample cavity for storage.
The application principle of the method is as follows:
under the application of the heat conduction characteristic of the material of the sample storage unit, the biological sample in the sample cavity can be effectively refrigerated at a low temperature through the arrangement of the cold source. Meanwhile, based on the fact that the thermal resistance of the low-thermal-resistance material is lower than that of the high-thermal-resistance material, the sample bin provided by the low-thermal-resistance material can ensure that the temperature of the sample bin in an extension size range is stable; under the influence of a cold source, the heat insulator of the high thermal resistance material combines the insulating and heat-insulating material arranged on the periphery to block heat insulation application, so that the unidirectional temperature gradient change condition from one end of the cold source contact to one end of the normal temperature heat source contact is formed; in the application scheme of combining the heat preservation body made of the high-thermal-resistance material and the heat conduction material of the sample bin made of the low-thermal-resistance material, different design applications of target temperature in a space in single constant cold source setting can be realized by designing different height positions of the sample bin in the heat preservation body, and the application requirements of storage temperatures of different biological samples are met.
Further, the application temperature of the cold source is in the range of-180 ℃ to-220 ℃; the application temperature of the normal-temperature heat source is in the range of 0-50 ℃; the application temperature of the temperature control area is in the range of 0 ℃ to-180 ℃.
Preferably, the application temperature of the temperature control zone is in the range of-130 ℃ to-150 ℃.
Further, the sample storage unit is made of a high thermal resistance material, and the thermal conductivity of the high thermal resistance material ranges from 0.02W/m.K to 1.00W/m.K.
Further, the low thermal resistance material used for the preparation of the sample bin has a material thermal conductivity in the range of
10W/m.K～500W/m.K。
Further, in the sample storage unit, the extension size range of the sample cavity from the cold source to the normal temperature heat source is larger than the target temperature interval size range of the temperature gradient change of the heat insulator from the cold source to the normal temperature heat source, which is independently arranged.
In practical application, for the heat insulator arranged by the conventional high thermal resistance material, the temperature decay is faster between the cold source and the normal temperature heat source, so that the size range of the temperature area of the target temperature for the application required by biological sample preservation in the temperature change is narrower. Therefore, a sample bin arranged in the heat preservation body needs to be applied to the sample storage unit, the extension size range of the set sample cavity is larger than the size range of the target temperature interval of the temperature gradient change in the independent heat preservation body, so that the target temperature application size range maintained by the temperature control area in the sample cavity can be applied in a wider temperature size area compared with the independent heat preservation body, the temperature stability of the temperature control area is ensured, the application range of the size area for the required design target temperature is effectively expanded, and the application requirement of storing the biological sample with the conventional size or the large size in the application of the small-volume storage device to the target temperature environment can be met.
Further, the position of the sample bin in the heat insulator along the direction from the cold source to the normal-temperature heat source is adjusted, so that the temperature of the region in the temperature control region is adjusted.
According to the design requirements of different biological samples, the position of the sample bin in the heat preservation body is adjusted by selecting, and the temperature in the sample cavity is adjusted and applied under the application of a single constant cold source.
Further, a temperature control heat source is arranged on the low thermal resistance material part in the sample bin, heating power of the temperature control heat source is adjusted, and the temperature of the area in the temperature control area is adjusted.
According to the design requirements of different biological samples, the temperature in the sample cavity is adjusted and applied under the application of a single constant cold source by selecting a temperature control heat source to carry out heating assistance.
In a preferred embodiment, a target storage temperature of the sample storage space is preset, the temperature in the sample bin is detected, and the temperature control heat source is controlled to generate heat according to the target storage temperature so as to control the temperature in the sample bin.
In another aspect, the invention provides a sample storage unit applied to the method for storing biological samples at low temperature, wherein the thermal insulation body comprises a thermal insulation shell made of high thermal resistance material, a thermal insulation cavity is arranged in the thermal insulation shell, a thermal insulation opening communicated with the thermal insulation cavity is arranged on the upper side of the thermal insulation shell, and a thermal insulation cover made of high thermal resistance material is detachably arranged on the thermal insulation opening; the sample bin is arranged between the heat preservation cavity and the heat preservation cover.
Further, the sample bin comprises a cylindrical temperature control cylinder made of low thermal resistance materials, and the bottom of the heat preservation cavity, the inner side of the temperature control cylinder and the bottom of the covered heat preservation cover are enclosed to form the sample cavity.
Further, the sample bin comprises a barrel bottom made of low thermal resistance materials, the barrel bottom is arranged on the upper side of the bottom of the heat preservation cavity, and the barrel bottom and the temperature control barrel are integrally formed.
Further, the whole heat preservation body is cylindrical, the whole sample cavity is cylindrical, and the sample cavity and the heat preservation body are coaxially arranged; the sample bin is arranged on the heat preservation body and close to the cold source contact part.
The invention provides a biological sample low-temperature storage method or a storage device with the sample storage unit, which comprises a first tank body made of an insulating and heat-insulating material, wherein the sample storage unit is arranged at the upper part in the first tank body, a cold source bin for placing a cold source is arranged at the lower part in the first tank body, a cold source inlet is arranged on the cold source bin, and the cold source inlet is communicated with the cold source inlet.
Further, a first cavity is formed in the first tank body, a first opening is formed in the upper side of the first cavity, the sample storage unit is positioned and assembled on the first opening and is placed in the first cavity, and the outer peripheral size and shape of the heat preservation body are matched with the inner cavity side size and shape of the first cavity; and the cold source bin is formed between the bottom of the heat insulating body and the first cavity.
Further, the first tank body is hollow and is provided with a hollow cavity, the cold source inlet is arranged on the upper portion of the outer wall of the first tank body, the cold source inlet is arranged on the lower portion of the inner wall of the first tank body, a first pipeline is arranged in the hollow cavity, and the cold source inlet is communicated with the cold source inlet through the first pipeline.
Further, the hollow cavity is arranged in vacuum. The vacuum hollow cavity can further meet the heat preservation application requirement in the tank body.
Specifically, the first tank body structure adopts a Dewar tank structure.
Further, the cold source is one or more liquid gases of nitrogen, argon or carbon dioxide.
The invention provides a biological sample low-temperature storage method or a transport device with the sample storage unit, which comprises a second tank body made of insulating materials, wherein the sample storage unit is arranged at the upper part in the second tank body, and a cold source installation area for positioning and installing a cold source structure is arranged at the lower part in the second tank body.
Further, the cold source structure comprises a first cold source structure made of liquid gas adsorption materials.
Further, the second tank body is provided with a second cavity, a second opening is formed in the upper side of the second cavity, the sample storage unit is positioned and assembled on the second opening and is placed in the second cavity, and the first cold source structure is positioned and installed at the lower part of the second cavity.
Further, the cavity inner diameter of the second cavity is larger than the outer diameter of the heat preservation body; the periphery of the upper side of the first cold source structure is vertically and upwardly extended to form a cold source wall, the cold source wall is matched with the periphery of the lower part of the heat preservation body, and the upper side of the first cold source structure is concavely provided to form an adaptation cavity adapted to the lower part of the heat preservation body.
The invention has the beneficial effects that:
1. the application of the biological sample low-temperature storage method can effectively design and apply according to the position of the sample bin under the condition of single constant cold source setting, can effectively form a temperature control area with target design temperature in the sample cavity of the biological sample storage method, and meets the biological sample storage application requirements of different storage temperatures.
2. The arrangement of the sample storage unit, the storage device and the transportation device can meet the demands of biological sample storage application and transportation application at different storage temperatures, and has the characteristics of simple application structure design and low cost.
Drawings
FIG. 1 is a schematic illustration of an application of a thermal insulator made of a high thermal resistance material;
FIG. 2 is a thermal analysis diagram of the insulation of FIG. 1 under the influence of a cold source and a normal temperature heat source;
FIG. 3 is a schematic illustration of the range of applied dimensions of a temperature gradient zone of a target storage temperature of a biological sample in the thermal insulation of FIG. 1;
FIG. 4 is a schematic diagram showing the structural application of a sample storage unit in the basic principle of the method for storing biological samples at low temperature according to the present invention;
FIG. 5 is a thermal analysis diagram of the sample storage unit of FIG. 4 under the influence of a cold source and a normal temperature heat source;
FIG. 6 is a schematic diagram showing the temperature in the sample chamber of the sample storage unit of FIG. 4 under the influence of a cold source and a normal temperature heat source;
FIG. 7 is a thermal analysis diagram of the sample storage unit of FIG. 4 under the influence of a cold source and a normal temperature heat source, wherein a temperature control heat source is used for heating;
FIG. 8 is a schematic diagram of temperature conditions in a sample chamber for heating application by using a temperature control heat source under the condition that the sample storage unit in FIG. 4 is affected by a cold source and a normal temperature heat source;
fig. 9 is a schematic structural view of a storage device according to the present invention;
fig. 10 is a schematic structural view of the transportation device of the present invention.
Reference numerals illustrate:
sample storage unit 1, heat preservation body 10, heat preservation shell 101, heat preservation cavity 102, heat preservation opening 103, heat preservation cover 104, sample bin 11, sample cavity 110, temperature control cylinder 111, cylinder bottom 112, heating sheet 12,
A cold source 2, a normal temperature heat source 3,
The first tank body 4, the first cavity 40, the first opening 401, the cold source bin 41, the cold source inlet 42, the cold source inlet 43, the hollow cavity 44, the first pipeline 45, the supporting leg structure 46,
The second tank body 5, the second cavity 50, the second opening 501, the cold source installation area 51, the first cold source structure 52, the cold source wall 521 and the adapting cavity 522.
Detailed Description
In order to make the technical solution, the objects and the advantages of the present invention more apparent, the present invention will be further explained with reference to fig. 1 to 10 and examples 1 to 3.
Example 1:
in this embodiment, the application principle of the method for storing a biological sample at a low temperature according to the present invention is illustrated.
Specifically, as shown in FIG. 1, an EPS foam insulator model (thermal conductivity: 0.02918 w/m.K) having a diameter of 300mm and a height of 500mm was designed for thermal analysis as an application of the thermal insulator 10. The top plane of the heat insulator 10 is a normal temperature heat source 3, which simulates the actual situation of interaction with the actual ambient temperature, the ambient temperature is 25 ℃, the heat is transferred by convection with air, and the heat transfer convection coefficient is set to 25W/m 2.K. A cold source 2 is applied to the bottom of the heat insulator 10, the constant temperature is-196 ℃ (liquid nitrogen temperature), a vacuum layer of Zhou Yingyong dewar outside the heat insulator 10 of the foam insulator model is used as insulation and heat insulation, and the fact that the periphery of the heat insulator 10 and the air environment do not have any heat exchange is assumed. The heat flow flows in from the upper end surface of the heat insulator 10 and flows out from the lower end surface. After steady state, the heat insulator 10 has a temperature gradient change from the cold source 2 to the normal temperature heat source 3.
After steady state, the temperature distribution of the heat insulator 10 is as shown in fig. 2 and 3.
The result shows that the heat flow is longitudinally distributed from the upper end face to the lower end face, the temperature of the foam insulating layer is from 24.336 ℃ of the upper end face to-196 ℃ of the lower end face, the target temperature required for designing the biological sample storage is from-130 ℃ to-150 ℃, and the temperature gradient arrangement size range of the foam insulating layer is only the area from 100mm to 150mm of the lower end face from the distance model, so that the foam insulating layer is very narrow in interval and is unfavorable for the storage of biological samples with conventional sizes.
As shown in fig. 4, the embodiment provides a basic sample storage unit 1 structure: on the basis of the heat preservation body 10 in fig. 1, a container (an aluminum container with the diameter of 250mm, the height of 200mm and the wall thickness of 5 mm) made of low thermal resistance material is placed at a position 70mm away from the bottom to expand the storage space, and a heating sheet 12 with adjustable power is added at the bottom of the aluminum container to serve as a temperature control heat source to control the temperature.
After steady state, the thermal analysis of the heat generating sheet 12 without operation is shown in fig. 5 and 6 (the abscissa in fig. 6 represents the position and the ordinate represents the temperature): after steady state, the average temperature of the air in the sample cavity 110 is-143.591 ℃, the highest temperature is-143.365 ℃, the lowest temperature is-143.818 ℃, and the temperature difference is 0.453 ℃, so that the target temperature application requirement of-130 ℃ to-150 ℃ is met.
The heat generating sheet 12 is selected to be energized with a power of 2w, and the application conditions are shown in fig. 7 and 8 (the abscissa in fig. 8 represents the position condition, and the ordinate represents the temperature condition): after steady state, the average temperature of the air in the sample chamber 110 was increased to-119.75 ℃, at-119.571 ℃ at maximum, at-119.919 ℃ at minimum, and at a temperature difference of 0.348 ℃. By adjusting the power of the heating plate 12 and combining a PID algorithm, the accurate regulation and control application of the target temperature in the sample cavity 110 can be realized, and the aim of temperature control is fulfilled.
Example 2:
as shown in fig. 9, based on the application principle of the above embodiment 1, the present embodiment provides a structural scheme of a storage device based on the storage application of biological samples.
Specifically, the storage device comprises a first tank body 4 (Dewar tank) made of an insulating and heat-insulating material, a sample storage unit 1 is arranged at the upper part in the first tank body 4, a cold source bin 41 for placing a cold source 2 is arranged at the lower part in the first tank body 4, a cold source inlet 42 is arranged on the first tank body 4, a cold source inlet 43 is arranged on the cold source bin 41, and the cold source inlet 42 is communicated with the cold source inlet 43.
The sample storage unit 1 comprises a heat preservation body 10 made of high-thermal-resistance materials, wherein the heat preservation body 10 comprises a heat preservation shell 101 made of high-thermal-resistance materials, a heat preservation cavity 102 is formed in the heat preservation shell 101, a heat preservation opening 103 communicated with the heat preservation cavity 102 is formed in the upper side of the heat preservation shell 101, and a heat preservation cover 104 made of the high-thermal-resistance materials is detachably arranged on the heat preservation opening 103; a sample bin 11 made of a low thermal resistance material is arranged between the thermal insulation cavity 102 and the thermal insulation cover 104.
The sample chamber 11 comprises a temperature control cylinder 111 made of low thermal resistance material, wherein the bottom of the heat preservation chamber 102, the inner side of the temperature control cylinder 111 and the bottom of the covered heat preservation cover 104 are enclosed to form the sample chamber 110 for placing biological samples. The set range inside the temperature control tube 111 is the extension range of the sample chamber 110. Through the vertically extending temperature control cylinder 111, in practical application, the inner side of the temperature control cylinder 111 can have the target stable temperature application condition. The formation of the temperature control region within the extension size range of the sample cavity 110 can be basically satisfied, and the setting requirement of temperature stability of the temperature control region is satisfied.
The whole thermal insulation body 10 is cylindrical, the whole sample cavity 110 surrounded by the temperature control cylinder 111 is also cylindrical, and the sample cavity 110 and the thermal insulation body 10 are coaxially arranged; the sample bin 11 is arranged at a part of the heat preservation body 10 close to the cold source contact part.
As a preferred embodiment, the sample compartment 11 further includes a bottom 112 made of a low thermal resistance material, the bottom 112 is disposed on the upper side of the bottom of the heat insulation cavity 102, and the bottom 112 and the temperature control barrel 111 are integrally formed; the heating sheet 12 is arranged on the lower side of the cylinder bottom 112; based on the structural combination of the barrel bottom 112 and the temperature control barrel 111, the temperature control area can have the effect of stabilizing the temperature; the application of the heating plate 12 as a temperature control heat source meets the requirement of further stable control of the temperature of the region in the sample cavity 110.
As a specific structural arrangement, the first tank 4 has a first cavity 40 at a central position, and a first opening 401 is provided on an upper side of the first cavity 40. Positioning and assembling the sample storage unit 1 on the first opening 401 and placing the sample storage unit into the first cavity 40, wherein the outer peripheral size and shape of the heat preservation body 10 are matched with the inner cavity size and shape of the first cavity 40; the cold source bin 41 is formed between the bottom of the heat insulator 10 and the first cavity 40.
The first tank body 4 is provided with a hollow cavity 44 in a hollow mode, and the hollow cavity 44 is provided with vacuum. The cold source inlet 42 is arranged on the upper part of the outer wall of the first tank body 4, the cold source inlet 43 is arranged on the lower part of the inner wall of the first tank body 4, a first pipeline 45 is arranged in the hollow cavity 44, and the cold source inlet 42 is communicated with the cold source inlet 43 through the first pipeline 45. The lower side of the first tank 4 is provided with a foot structure 46.
In practical application, the external heat sink 2 may be input through the heat sink inlet 42, and then input into the heat sink bin 41 through the first pipe 45 and the heat sink inlet 43, so as to satisfy the input application of the heat sink 2. The cold source 2 is one or more liquid gases selected from nitrogen, argon or carbon dioxide.
In the application of the transportation device, the temperature of the environment contacted with the upper side of the heat preservation body 10 is taken as a normal temperature heat source 3, and liquid gases such as nitrogen, argon, carbon dioxide and the like are taken as a cold source 2, so that the heat flow of the whole sample storage unit 1 can flow through an insulating material with high thermal resistance to generate a temperature gradient. And then, according to different biological sample storage conditions, the application design of the target temperature is carried out, and the position of the sample bin 11 is set by selecting a specific temperature gradient interval position, so that the temperature control region of the sample cavity 110 in the sample bin 11 can be used as a refrigerating region of a corresponding biological sample.
On the other hand, for the sample bin 11 made of the material with low thermal resistance, the purposes of reducing the temperature difference of the temperature control area and increasing the storage space of the extending size range of the sample cavity 110 can be achieved by designing a proper wall thickness, increasing the heat conducting area or designing a heat transfer structure and reducing the thermal resistance of the temperature control area.
Example 3:
as shown in fig. 10, in order to meet the transportation application requirement of the biological sample, the present embodiment provides a transportation device structure for the transportation limitation of the cold source 2 in the existing transportation vehicle.
Specifically, the transportation device comprises a second tank body 5 (dewar tank) made of insulating and heat-insulating materials, the upper part in the second tank body 5 is provided with a sample storage unit 1 structure application as the application principle, and the lower part in the first tank body 4 is provided with a cold source installation area 51 for positioning and installing a cold source 2 structure.
The cold source 2 structure comprises a first cold source structure 52 made of liquid gas adsorption materials; the second tank body 5 is provided with a second cavity 50, a second opening 501 is formed in the upper side of the second cavity 50, the sample storage unit 1 is positioned and assembled on the second opening 501 and is placed in the second cavity 50, and the first cold source structure 52 is positioned and installed at the lower part of the second cavity 50.
As a preferred embodiment, the cavity inner diameter of the second cavity 50 is larger than the outer diameter of the heat insulator 10; the upper side periphery of the first cold source structure 52 extends vertically upwards to form a cold source wall 521, the cold source wall is matched with the lower periphery of the heat insulation body 10, and an adaptation cavity 522 matched with the lower part of the heat insulation body 10 in shape is concavely formed on the upper side of the first cold source structure 52.
The first cold source structure 52 is optionally applied as a liquid nitrogen adsorption material, so that the corresponding liquid nitrogen adsorption material is arranged in the second tank body 5 for application after absorbing the liquid nitrogen cold source 2, the risk of liquid nitrogen dumping and overflowing in the transportation process can be effectively avoided, the transportation device can be conveniently applied to different transportation means, and the transportation requirement of biological samples is met.
The foregoing is merely a preferred embodiment of the present invention, and modifications of the embodiments described above can be made by those skilled in the art without departing from the implementation principles of the present invention, and the corresponding modifications should also be considered as the protection scope of the present invention.
Claims (20)
1. A method for the cryogenic storage of biological samples, comprising the steps of:
s1, setting a sample storage unit, wherein the sample storage unit comprises a heat insulation body made of a high thermal resistance material, and the high thermal resistance material used for preparing the heat insulation body has a material thermal conductivity range of 0.02W/m.K-1.00W/m.K; arranging an insulating and heat-insulating material on the periphery of the sample storage unit, wherein when one end of the heat insulator is contacted with a cold source and the other end of the heat insulator is contacted with a normal-temperature heat source, the heat insulator has a temperature gradient change condition from the cold source to the normal-temperature heat source;
s2, arranging a sample bin made of a low-thermal-resistance material in the heat preservation body, wherein the thermal conductivity of the low-thermal-resistance material prepared by the sample bin is 10W/m.K-500W/m.K; the sample bin is internally provided with a sample cavity for placing biological samples; the sample cavity is provided with an extension size range from the cold source to the normal temperature heat source; when one end of the heat insulator is contacted with a cold source and the other end is contacted with a normal-temperature heat source, the sample cavity forms a temperature control area with stable temperature in the extension size range under the heat conduction effect of a low-thermal-resistance material applied to the sample bin; adjusting the position of the sample bin in the heat preservation body along the direction from the cold source to the normal-temperature heat source, so that the temperature of the region in the temperature control region is adjusted;
s3, placing the sample storage unit between a cold source and a normal-temperature heat source, and placing a biological sample into a sample cavity for storage;
in the sample storage unit, the extension size range of the sample cavity from the cold source to the normal temperature heat source is larger than the target temperature interval size range of the temperature gradient change of the heat preservation body from the cold source to the normal temperature heat source, which is independently arranged.
2. The method of claim 1, wherein the cold source is applied at a temperature ranging from-180 ℃ to-220 ℃.
3. The method for the cryogenic storage of biological samples of claim 1, wherein the application temperature of said ambient heat source is in the range of 0 ℃ to 50 ℃.
4. The method of claim 1, wherein the temperature-controlled region is applied at a temperature ranging from 0 ℃ to-180 ℃.
5. The method of claim 4, wherein the temperature-controlled region is applied at a temperature ranging from-130 ℃ to-150 ℃.
6. The method according to any one of claims 1 to 5, wherein a temperature control heat source is provided to the low thermal resistance material portion in the sample chamber, and the heating power of the temperature control heat source is adjusted to adjust the temperature of the region in the temperature control region.
7. The method according to claim 6, wherein a target storage temperature of the sample storage space is preset, the temperature in the sample compartment is detected, and the temperature control heat source is controlled to generate heat according to the target storage temperature, so that the temperature in the sample compartment is controlled.
8. The sample storage unit applied to the biological sample low-temperature storage method according to any one of claims 1 to 7, wherein the heat preservation body comprises a heat preservation shell made of high-thermal-resistance materials, a heat preservation cavity is arranged in the heat preservation shell, a heat preservation opening communicated with the heat preservation cavity is arranged on the upper side of the heat preservation shell, and a heat preservation cover made of high-thermal-resistance materials is detachably arranged on the heat preservation opening; the sample bin is arranged between the heat preservation cavity and the heat preservation cover.
9. The biological sample storage unit of claim 8, wherein the sample bin comprises a temperature control cylinder made of low thermal resistance materials, and the bottom of the heat preservation cavity, the inner side of the temperature control cylinder and the bottom of the covered heat preservation cover are enclosed to form the sample cavity.
10. The biological sample storage unit of claim 9, wherein the sample compartment comprises a bottom made of a low thermal resistance material, the bottom being disposed on the upper side of the bottom of the thermal chamber, the bottom being integrally formed with the temperature control cartridge.
11. The biological sample storage unit of claim 8, wherein the thermal insulator is integrally provided in a cylindrical shape, the sample chamber is integrally provided in a cylindrical shape, and the sample chamber is coaxially provided with the thermal insulator; the sample bin is arranged on the heat preservation body and close to the cold source contact part.
12. The storage device for the biological samples by using the low-temperature storage method according to any one of claims 1 to 7 or the sample storage unit according to any one of claims 8 to 11 is characterized by comprising a first tank body made of an insulating and heat-insulating material, wherein the sample storage unit is arranged at the upper part in the first tank body, a cold source bin for placing a cold source is arranged at the lower part in the first tank body, a cold source inlet is arranged on the cold source bin, and the cold source inlet is communicated with the cold source inlet.
13. The storage device according to claim 12, wherein the first tank has a first cavity therein, a first opening is provided on an upper side of the first cavity, the sample storage unit is positioned and assembled on the first opening and is placed in the first cavity, and a peripheral size shape of the heat insulator is adapted to a cavity inner side size shape of the first cavity; and the cold source bin is formed between the bottom of the heat insulating body and the first cavity.
14. The storage device of claim 13, wherein the first tank is hollow with a hollow cavity, the cold source inlet is disposed on an upper portion of an outer wall of the first tank, the cold source inlet is disposed on a lower portion of an inner wall of the first tank, a first pipeline is disposed in the hollow cavity, and the cold source inlet is communicated with the cold source inlet through the first pipeline.
15. The storage device of claim 14 wherein said hollow cavity is vacuum disposed therein.
16. The storage device of claim 12, wherein the cold source is one or more liquid gases of nitrogen, argon, or carbon dioxide.
17. The method for storing biological samples at low temperature according to any one of claims 1 to 7 or the transportation device using the sample storage unit according to any one of claims 8 to 11, characterized by comprising a second tank body made of insulating and heat-insulating material, wherein the sample storage unit is arranged at the upper part in the second tank body, and a cold source installation area for positioning and installing a cold source structure is arranged at the lower part in the second tank body.
18. The transport apparatus of claim 17, wherein the cold source structure comprises a first cold source structure fabricated from a liquid gas adsorbent material.
19. The transport apparatus of claim 18, wherein the second tank has a second cavity therein, a second opening is provided on an upper side of the second cavity, the sample storage unit is positioned and assembled on the second opening and is placed in the second cavity, and the first cold source structure is positioned and installed on a lower portion of the second cavity.
20. The transport apparatus of claim 19, wherein the second cavity has a cavity inner diameter greater than an outer diameter of the insulation; the periphery of the upper side of the first cold source structure is vertically and upwardly extended to form a cold source wall, the cold source wall is matched with the periphery of the lower part of the heat preservation body, and the upper side of the first cold source structure is concavely provided to form an adaptation cavity adapted to the lower part of the heat preservation body.
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| US6209343B1 (en) * | 1998-09-29 | 2001-04-03 | Life Science Holdings, Inc. | Portable apparatus for storing and/or transporting biological samples, tissues and/or organs |
| CN106030223A (en) * | 2014-02-18 | 2016-10-12 | Cbcest株式会社 | Temperature-regulated transport box |
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| MX2022012190A (en) * | 2020-03-31 | 2022-11-30 | X Therma Inc | Apparatus for biological material storage and transport. |
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|---|---|---|---|---|
| US6209343B1 (en) * | 1998-09-29 | 2001-04-03 | Life Science Holdings, Inc. | Portable apparatus for storing and/or transporting biological samples, tissues and/or organs |
| CN106030223A (en) * | 2014-02-18 | 2016-10-12 | Cbcest株式会社 | Temperature-regulated transport box |
| JP2018197629A (en) * | 2017-05-24 | 2018-12-13 | Ｐｈｃホールディングス株式会社 | Storage device |
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