CN116067849A

CN116067849A - Blood analyzer and blood detection method

Info

Publication number: CN116067849A
Application number: CN202111272147.2A
Authority: CN
Inventors: 麦华福
Original assignee: Shenzhen Mindray Bio Medical Electronics Co Ltd
Current assignee: Shenzhen Mindray Bio Medical Electronics Co Ltd
Priority date: 2021-10-29
Filing date: 2021-10-29
Publication date: 2023-05-05

Abstract

The present disclosure provides a blood analyzer and a blood detection method, the blood analyzer including a sampling device, a detection assembly, a receiving portion, and a liquid path support assembly. The sampling device is configured to collect a sample to be measured. The detection assembly at least comprises a detection pipeline, a first detection part for detecting blood sedimentation and a second detection part for detecting hemoglobin, wherein the first detection part is arranged on the detection pipeline. The accommodating part is configured to receive a sample to be detected in the sampling device and/or the detection pipeline, the detection pipeline is connected to the accommodating part, and the second detection part is sleeved outside the accommodating part. The liquid path support component is configured to provide liquid path support among the sampling device, the accommodating part and the detection pipeline so as to obtain the detection result of hemoglobin and blood sedimentation together for the sample to be detected. The blood analyzer can realize the detection of HGB and ESR shared blood segments, and achieves the purpose of saving blood volume.

Description

Blood analyzer and blood detection method

Technical Field

The present disclosure relates to the technical field of medical devices, and in particular, to a blood analyzer and a blood detection method.

Background

Currently, blood analyzers are mainly used for conventional detection of blood, and can detect basic parameters of blood such as erythrocyte count, white blood cell count and classification (three-class or five-class), platelet count, hemoglobin (HGB) content, erythrocyte Sedimentation Rate (ESR), and some clinically significant parameters calculated from these parameters. At present, the HGB and the ESR are often detected separately and independently, so that the blood analyzer needs more blood volume to detect the HGB and the ESR, which can cause the situations of large blood volume requirement and waste of blood volume.

Disclosure of Invention

Aiming at the technical problems in the prior art, the present disclosure provides a blood analyzer and a blood detection method, which can realize the detection of HGB and ESR shared blood segments, so as to achieve the purpose of saving blood volume.

In a first aspect, embodiments of the present disclosure provide a blood analyzer including a sampling device, a detection assembly, a receptacle, and a fluid path support assembly. The sampling device is configured to collect a sample to be measured. The detection assembly at least comprises a detection pipeline, a first detection part for detecting blood sedimentation and a second detection part for detecting hemoglobin, wherein the first detection part is arranged on the detection pipeline. The accommodating part is configured to receive a sample to be detected in the sampling device and/or the detection pipeline, the detection pipeline is connected to the accommodating part, and the second detection part is sleeved outside the accommodating part. The liquid path support component is configured to provide liquid path support among the sampling device, the accommodating part and the detection pipeline so as to obtain the detection result of hemoglobin and blood sedimentation together for the sample to be detected.

In a second aspect, embodiments of the present disclosure further provide a blood testing method, applied to a blood analyzer, where the blood analyzer includes a sampling device, a containing portion, a testing assembly, and a liquid path supporting assembly, where the testing assembly includes at least a testing pipeline, a first testing portion for testing blood sedimentation, and a second testing portion for testing hemoglobin, and the first testing portion is disposed on the testing pipeline; the method comprises the following steps: collecting a sample to be tested via the sampling device; receiving a sample to be tested from the sampling device and/or the detection pipeline through the accommodating part; the second detection part is sleeved outside the accommodating part; and providing liquid path support among the sampling device, the accommodating part and the detection pipeline through the liquid path support component so as to obtain the detection result of hemoglobin and blood sedimentation together for the sample to be detected.

Compared with the prior art, the beneficial effects of the embodiment of the disclosure are that: according to the blood sedimentation detection device, the first detection part is used for detecting the blood sedimentation of the sample to be detected in the detection pipeline, and the second detection part can be used for detecting the hemoglobin of the sample to be detected in the accommodating part after the blood sedimentation detection is finished, so that the blood analyzer can utilize the blood segment used in detecting the blood sedimentation when detecting the hemoglobin, the blood volume requirement of the blood analyzer is reduced, and the purpose of saving the blood volume is achieved.

Drawings

In the drawings, which are not necessarily drawn to scale, like numerals may describe similar components in different views. The same reference numerals with letter suffixes or different letter suffixes may represent different instances of similar components. The accompanying drawings illustrate various embodiments by way of example in general and not by way of limitation, and together with the description and claims serve to explain the disclosed embodiments. Wherever possible, the same reference numbers will be used throughout the drawings to refer to the same or like parts. Such embodiments are illustrative and not intended to be exhaustive or exclusive of the present apparatus or method.

FIG. 1 is a block diagram of a blood analyzer according to an embodiment of the present disclosure;

FIG. 2 is a first partial block diagram of a blood analyzer according to an embodiment of the present disclosure;

FIG. 3 is a second partial block diagram of a blood analyzer according to an embodiment of the present disclosure;

FIG. 4 is a schematic structural view of a third detecting section of the blood analyzer according to the embodiment of the present disclosure;

fig. 5 is a flow chart of a blood testing method according to an embodiment of the present disclosure.

The reference numerals in the drawings denote components:

100-hematology analyzer; 1-a sampling device; 21-a first detection section; 22-a second detection section; 23-detecting a pipeline; 24-a third detection section; 241-counting cell; 242-gemstone hole; 243-electrode; 244-direct current power supply; 245-an amplifier; 3-a housing; 4-a liquid path support assembly; 5-a control device; 6-a conveying pipeline.

Detailed Description

In order to better understand the technical solutions of the present disclosure, the following detailed description of the present disclosure is provided with reference to the accompanying drawings and the specific embodiments. Embodiments of the present disclosure will be described in further detail below with reference to the drawings and specific embodiments, but not by way of limitation of the present disclosure.

The terms "first," "second," and the like, as used in this disclosure, do not denote any order, quantity, or importance, but rather are used to distinguish one element from another. The word "comprising" or "comprises" and the like means that elements preceding the word encompass the elements recited after the word, and not exclude the possibility of also encompassing other elements. "upper", "lower", "left", "right", etc. are used merely to indicate relative positional relationships, which may also be changed when the absolute position of the object to be described is changed.

In this disclosure, when a particular device is described as being located between a first device and a second device, there may or may not be an intervening device between the particular device and either the first device or the second device. When it is described that a particular device is connected to other devices, the particular device may be directly connected to the other devices without intervening devices, or may be directly connected to the other devices without intervening devices.

All terms (including technical or scientific terms) used in this disclosure have the same meaning as commonly understood by one of ordinary skill in the art to which this disclosure belongs, unless specifically defined otherwise. It will be further understood that terms, such as those defined in commonly used dictionaries, should be interpreted as having a meaning that is consistent with their meaning in the context of the relevant art and will not be interpreted in an idealized or overly formal sense unless expressly so defined herein.

Techniques, methods, and apparatus known to one of ordinary skill in the relevant art may not be discussed in detail, but are intended to be part of the specification where appropriate.

The presently disclosed embodiments provide a blood analyzer 100, as shown in fig. 1 and 2, the blood analyzer 100 including a sampling device 1, a detection assembly, a housing 3, and a fluid path support assembly 4. Specifically, the blood analyzer 100 may further include a control device 5, where the control device 5 may be electrically connected to the sampling device 1, the detection assembly, and the fluid path support assembly 4, respectively, to perform information processing and control and coordinate operations of the respective components. In some embodiments, the control device 5 may be implemented in various ways, such as, but not limited to, the control device 5 including at least a processing component, RAM, ROM, a communication interface, memory, and an I/O interface. The processing components, RAM, ROM, communications interfaces, memory, and I/O interfaces communicate over a bus. The processing component may be a CPU, GPU or other chip with computing capabilities. The memory stores various computer programs such as an operating system and application programs for execution by the processor element and data required for execution of the computer programs. In addition, during the blood sample analysis, data stored locally may be stored in memory if desired. The I/O interface is constituted by a serial interface such as USB, IEEE1394, or RS-232C, a parallel interface such as SCSI, IDE, or IEEE1284, and an analog signal interface composed of a D/a converter, an a/D converter, and the like. An input device consisting of a keyboard, mouse, touch screen or other control buttons is connected to the I/O interface, and a user can directly input data to the control device 5 using the input device. In addition, a display device having a display function, for example, may be connected to the I/O interface: liquid crystal screens, touch screens, LED display screens, and the like. The control device 5 may output the processed data as image display data to a display device for display, for example: analytical data, instrument operating parameters, etc. The communication interface is an interface that may be any communication protocol presently known. The communication interface communicates with the outside through a network. The control device 5 can transmit data via a communication interface with any device connected via the network in a certain communication protocol.

In addition, the blood analyzer 100 may further include a protein detection module or other detection modules for detecting a specific protein, which is not particularly limited herein, and other detection modules may be adaptively added according to actual detection requirements.

The sampling device 1 described above is configured to collect a sample to be measured. Specifically, the sampling device 1 is used for collecting a blood sample from a sample container containing the blood sample and dispensing the blood sample to a detection assembly. The sampling device 1 described above may comprise a sampling needle for aspirating a sample to be tested.

Further, the detecting assembly comprises at least a detecting pipeline 23, a first detecting part 21 for detecting blood sedimentation and a second detecting part 22 for detecting hemoglobin, wherein the first detecting part 21 is arranged on the detecting pipeline 23. The detection pipeline 23 of the detection assembly is used for providing a detection place for blood sedimentation of a sample to be detected. The first detecting portion 21 may include an optical detecting module to irradiate the sample to be detected in the detecting pipeline 23, so as to determine the erythrocyte sedimentation rate of the sample to be detected according to the absorption or scattering degree of the sample to be detected in the detecting pipeline 23. The first detecting portion 21 may further include a heater and a temperature sensor, and temperature control of the detecting line 23 is achieved by the heater and the temperature sensor.

Further, the accommodating portion 3 is configured to receive a sample to be measured in the sampling device 1 and/or the detection pipeline 23, the detection pipeline 23 is connected to the accommodating portion 3, and the second detection portion 22 is sleeved outside the accommodating portion 3. The liquid path support assembly 4 is configured to provide liquid path support between the sampling device 1, the accommodating portion 3 and the detection line 23 so as to obtain a detection result of hemoglobin together with blood sedimentation for the sample to be measured.

Specifically, the fluid circuit support assembly 4 may include functional support such as fluid actuation, reagent priming, fluid circuit cleaning, waste fluid drainage, and the like. For example, the liquid path support assembly 4 can provide cleaning liquid for the sampling device 1, the accommodating portion 3 and the detecting assembly, so as to clean the sampling device 1, the accommodating portion 3 and the detecting pipeline 23, respectively, to avoid polluting the blood sample to be detected and causing inaccurate detecting result. The liquid path support component 4 can also be connected with the sampling needle through a pipeline so as to keep the pipeline full of diluent, so that the processes of sampling, sample dividing and the like are realized more quickly and reliably. The hydraulic support assembly 4 may include a pump, syringe or other source of power-able pressure, such as a source of positive and negative air pressure, or the like.

Specifically, the blood segment to be tested in the test tube 23 may directly flow into the test tube 23 through the sampling device 1 under the driving of the liquid support assembly 4, so that the first detecting portion 21 can detect the blood segment to be tested in the test tube 23. After the first detecting portion 21 detects the blood sedimentation, the sample to be detected in the detecting pipeline 23 can be transported into the accommodating portion 3 through the liquid path supporting component 4, and the sample to be detected is supplied to the accommodating portion 3 through the detecting pipeline 23, so that the hemoglobin of the sample to be detected in the accommodating portion 3 is detected through the second detecting portion 22. In this case, as an alternative embodiment, the test line 23 can be connected directly to the sampling needle of the sampling device 1, so that after the sample to be tested is pulled into the test line 23, the blood sedimentation of the sample to be tested can be detected directly by the first detection part 21.

Specifically, the above-mentioned liquid path supporting component 4 may also transport the sample to be measured into the accommodating portion 3 via the sampling device 1, and then transport the sample to be measured in the accommodating portion 3 into the detecting pipeline 23 connected to the accommodating portion 3, where the first detecting portion 21 may detect the sample piece to be measured in the detecting pipeline 23, and at this time, the detecting pipeline 23 may be connected to the accommodating portion 3 and have a communicating relationship, so that the sample to be measured in the accommodating portion 3 may be transported into the detecting pipeline 23. After the first detecting unit 21 detects the blood sedimentation, the sample to be detected in the detecting tube 23 can be transported into the accommodating unit 3 via the liquid path supporting unit 4, and at this time, the sample to be detected in the accommodating unit 3 comes from the sampling device 1 and the detecting tube 23, and then the hemoglobin of the sample to be detected in the accommodating unit 3 is detected by the second detecting unit 22. Thus, the detection results of hemoglobin and blood sedimentation of the sample to be detected are obtained together.

In combination with the above, the present disclosure detects the blood sedimentation of the sample to be detected in the detection pipeline 23 through the first detection portion 21, and the second detection portion 22 can detect the hemoglobin of the sample to be detected in the containing portion 3 after the blood sedimentation detection is finished, so that the blood analyzer 100 can utilize the blood segment used in detecting the blood sedimentation when detecting the hemoglobin, thereby reducing the blood volume requirement of the blood analyzer 100 and achieving the purpose of saving the blood volume.

In addition, when the sample to be tested in the test line 23 comes from the accommodating portion 3, the sample can be returned to the accommodating portion 3 after blood sedimentation test to prepare for subsequent hemoglobin test; the sample to be measured can also be supplied into the accommodating portion 3 through the detection pipeline 23 via the sampling device 1, so that blood sedimentation detection can be realized when the sample to be measured is supplied into the accommodating portion 3, and detection of blood sedimentation and hemoglobin can be realized through the shared blood segment. In addition, the blood sedimentation detection and the hemoglobin detection are measured through a set of pipelines, so that the problem of variation of measurement results caused by assembly errors and machining errors of equipment can be reduced as much as possible.

In some embodiments, the detection line 23 is connected to a sampling needle of the sampling device 1. The liquid path support assembly 4 is further configured to: the sample to be measured collected by the sampling device 1 is pumped into the detection pipeline 23. The first detecting section 21 is configured to: and detecting the blood sedimentation of the sample to be detected after the sample to be detected reaches the detection pipeline 23.

Specifically, the sampling needle has an inlet side and an outlet side, and under the power support of the liquid path support assembly 4, the sampling needle sucks the sample to be tested in the sample container through the inlet side thereof, the outlet side thereof can be directly connected with the detection pipeline 23, the detection pipeline 23 can also be integrated on the outlet side of the sampling needle, and the liquid path support assembly 4 can directly drag the sample to be tested into the detection pipeline 23 through the sampling needle.

Specifically, after the sample to be measured reaches the inside of the detection line 23, the control device 5 may control the first detection portion 21 to enter a detection state, so as to detect the blood sedimentation of the sample to be measured via the first detection portion 21.

In some embodiments, the liquid path support assembly 4 is further configured to: the sample to be measured collected by the sampling device 1 is drawn into the accommodating portion 3, and the sample to be measured in the accommodating portion 3 is drawn into the detection pipeline 23. The first detecting section 21 is configured to: after the sample to be measured reaches the detection line 23 via the housing 3, the blood sedimentation of the sample to be measured is detected.

Specifically, the accommodating portion 3 may be connected to the sampling device 1 through a communication pipeline, and the sample to be tested is transported into the accommodating portion 3 through the communication pipeline by the sampling device 1 under the power support of the liquid path support assembly 4, and then the sample to be tested in the accommodating portion 3 is dragged into the detecting pipeline 23 connected to the accommodating portion 3, so as to be detected by the first detecting portion 21.

Specifically, the above-mentioned communication line and the detection line 23 are different lines, which are respectively connected to different positions on the housing portion 3.

In some embodiments, the liquid path support assembly 4 is further configured to: after the first detecting portion 21 detects blood sedimentation, the blood segment to be measured in the detecting line 23 is returned to the accommodating portion 3, and a diluent is supplied into the accommodating portion 3.

Specifically, after the first detection part 21 completes the blood sedimentation detection, the to-be-detected blood segment in the detection pipeline 23 can be spit back into the accommodating part 3 under the power support of the liquid path support component 4, so that the second detection part 22 can detect the blood segment used in the blood sedimentation by using the first detection part 21 when detecting the hemoglobin, thereby realizing the sharing of the blood segment and saving of the blood segment.

Specifically, the above-mentioned liquid path support assembly 4 can feed the diluting liquid into the accommodating portion 3 through other pipelines while spitting the blood segment to be measured in the detection pipeline 23 back to the accommodating portion 3, so as to accelerate the dilution efficiency of the sample to be measured in the accommodating portion 3. Of course, the diluent may be supplied into the housing portion 3 before the blood segment to be measured is returned, or the diluent may be supplied into the housing portion 3 after the blood segment to be measured is returned. The time of the blood return spitting section and the time of the diluent adding are not particularly limited, and the diluted sample to be tested can be prepared.

In some embodiments, the liquid path support assembly 4 is further configured to: after the diluent is supplied into the housing part 3, a hemolytic agent is added to the diluted sample to be measured to form a test sample that can be used for detecting hemoglobin. The second detecting section 22 is configured to: after the formation of the test sample, hemoglobin of the test sample in the housing part 3 is detected.

Specifically, in order to prepare a test sample capable of detecting hemoglobin, the hemolytic agent is added into the accommodating portion 3 through the liquid path supporting component 4, the time sequence of adding the hemolytic agent and the diluent is not limited, the hemolytic agent can be added first, then the diluent can be added, or the diluent can be added first, then the hemolytic agent can be added, and of course, the hemolytic agent and the diluent can be added into the accommodating portion 3 at the same time, which is not particularly limited in this application.

Specifically, the accommodating portion 3 can provide a reaction field for the sample to be measured, the diluent, and the hemolysis agent so as to prepare a sample for detecting hemoglobin, so as to prepare for the second detecting portion 22 to detect hemoglobin. The hemolytic agent is, for example, a hemolytic agent capable of dissolving red blood cells in a sample to be measured, releasing hemoglobin in the red blood cells, and converting hemoglobin into methemoglobin.

In combination with the above, when the blood sedimentation and hemoglobin are required to be detected by the blood analyzer 100, the sample to be detected can be dragged into the detection pipeline 23 by the sampling device 1, the sample to be detected in the accommodating portion 3 can be dragged into the detection pipeline 23, and after the blood sedimentation detection is performed on the blood segment to be detected in the detection pipeline 23 by the first detection portion 21, the sample to be detected in the detection pipeline 23 is spitted into the accommodating portion 3. The sample to be measured in the housing portion 3 is diluted, and a hemolytic agent is added to the housing portion 3 to prepare a test sample capable of measuring hemoglobin, and then the hemoglobin of the test sample is detected by the second detecting portion 22. Thus realizing the measurement of the blood sedimentation and the hemoglobin of the sample to be measured on the basis of sharing blood segments.

In some embodiments, as shown in fig. 3 and 4, the detecting assembly further includes a third detecting part 24 for detecting red blood cells, the third detecting part 24 includes a counting cell 241 having a jewel hole 242 with an electrode 243, and the counting cell 241 is separately or integrally disposed from the receiving part 3.

Specifically, the third detecting unit 24 may measure red blood cells by an impedance method, that is, the third detecting unit 24 may be configured as an impedance detecting unit for detecting a diluted sample to be measured to obtain red blood cell parameters and platelet parameters, which specifically includes the counting cell 241 having the jewel hole 242.

Specifically, after the sample to be measured is sucked by the sampling device 1, the sample to be measured is made to enter the accommodating portion 3 through the detection pipeline 23 under the power support of the liquid path support assembly 4, or the sample to be measured in the accommodating portion 3 is made to enter the communicating pipeline, the blood sedimentation detection is completed in the first detection portion 21, the sample to be measured in the detection pipeline 23 is discharged into the accommodating portion 3, and at this time, the diluent can be added into the accommodating portion 3 to prepare the sample to be measured which can be used for detecting the red blood cells after dilution. The diluted sample to be measured in the housing part 3 is fed to the impedance detecting part, that is, to the counting cell 241 via the liquid path supporting member 4, and the red blood cells of the diluted sample to be measured are detected by the impedance detecting part.

Specifically, as shown in fig. 4, the counting cell 241 may be provided independently of the housing portion 3, and the diluted sample to be measured may be supplied to the counting cell 241 through the conveying line 6 communicating with the housing portion 3. The electrodes 243 of the jewel hole 242 are electrically connected to a dc power supply 244, and the dc power supply 244 supplies dc power between the pair of electrodes 243. During the period when the dc power supply 244 supplies dc power, the impedance between the pair of electrodes 243 can be detected. The resistance signal indicating the change in impedance is amplified by the amplifier 245 and then supplied to the control device 5. The magnitude of the resistance signal corresponds to the volume (size) of the particles, so that the red blood cell parameter and the platelet parameter of the sample to be measured can be obtained by signal processing of the resistance signal by the control device 5.

Specifically, the counting cell 241 may be integrally formed with the accommodating portion 3, so that the diluted sample to be measured in the accommodating portion 3 may enter the counting cell 241 for erythrocyte detection.

In some embodiments, a sheath fluid inlet is provided on the counting cell 241 of the third detecting portion 24, so that the sample to be detected is wrapped by the sheath fluid flowing from the sheath fluid inlet and passes through the jewel hole 242 of the counting cell 241, so as to detect the red blood cells of the sample to be detected.

Specifically, the above-described third detecting portion 24 may also be configured as a sheath flow resistance detecting portion provided with a sheath liquid inlet (not shown in the drawing) on the counting cell 241 and may also be provided with a sheath liquid tank (not shown in the drawing) for supplying sheath liquid to the counting cell 241. In the counting cell 241, the sample to be measured flows through the jewel hole 242 under the wrapping of the sheath liquid to change the sample to be measured into a thin stream, so that particles (formed components) contained in the sample to be measured pass through the jewel hole 242 one by one. Then, by detecting the impedance between the pair of electrodes 243, a resistance signal indicating a change in impedance is obtained, and the resistance signal is amplified by the amplifier 245 and sent to the control device 5. The magnitude of the resistance signal corresponds to the volume (size) of the particles, so that the red blood cell parameter and the platelet parameter of the sample to be measured can be obtained by signal processing of the resistance signal by the control device 5.

In some embodiments, the liquid path support assembly 4 is further configured to: and pumping the diluted blood segment to be measured into the counting cell 241. The third detecting section 24 is configured to: and detecting direct current impedance generated when particles in the diluted sample to be detected pass through the jewel hole 242 of the counting cell 241, and outputting an electric signal reflecting information when the particles pass through the jewel hole 242 so as to detect erythrocytes of the diluted sample to be detected.

In some embodiments, the liquid path support assembly 4 is further configured to: after the third detecting unit 24 completes the detection of the red blood cells, a hemolytic agent is added to the diluted sample to be tested to form a detection sample that can be used for detecting hemoglobin. The second detecting section 22 is configured to: after the test sample is formed, hemoglobin of the test sample is detected.

The third detecting unit 24 detects the diluted sample to be detected, the second detecting unit 22 detects the diluted sample to be detected with the hemolytic agent added, and the sample to be detected cannot be subjected to blood sedimentation or erythrocyte detection after the hemolytic agent is added to the sample to be detected, so that in the case of blood sedimentation detection, erythrocyte detection and hemoglobin detection sharing the blood segment, the blood sedimentation detection and erythrocyte detection occur before the hemoglobin detection, and the blood sedimentation is detected on the sample to be detected before the dilution, and thus the blood sedimentation detection occurs before the erythrocyte detection.

In view of the above, when the blood sedimentation, the red blood cells, and the hemoglobin need to be detected by the blood analyzer 100, the present application first detects the blood sedimentation of the sample to be detected in the detection line 23 via the first detection unit 21, and then spits the sample to be detected in the detection line 23 into the housing unit 3 after the blood sedimentation detection is completed. The sample to be measured in the housing portion 3 is diluted, and then the diluted sample to be measured in the housing portion 3 is subjected to erythrocyte detection via the third detecting portion 24. After completion of the erythrocyte measurement, a hemolytic agent is added to the housing portion 3 to prepare a measurement sample capable of measuring hemoglobin, and the second measurement portion 22 is used to measure hemoglobin of the measurement sample. Thereby realizing the measurement of the blood sedimentation, the red blood cells and the hemoglobin of the sample to be measured on the basis of sharing the blood segment.

The embodiment of the present disclosure also provides a blood detection method, which is applied to the blood analyzer 100, where the blood analyzer 100 includes a sampling device 1, a containing portion 3, a detection assembly and a liquid path support assembly 4, the detection assembly includes at least a detection pipeline 23, a first detection portion 21 for detecting blood sedimentation, and a second detection portion 22 for detecting hemoglobin, and the first detection portion 21 is disposed on the detection pipeline 23.

Further, as shown in fig. 5, the blood detection method includes steps S101 to S103.

Step S101: a sample to be measured is collected via the sampling device 1.

Step S102: receiving a sample to be measured from the sampling device 1 and/or the detection line 23 via the receiving portion 3; the detection pipeline 23 is connected to the accommodating portion 3, and the second detection portion 22 is sleeved outside the accommodating portion 3.

Step S103: liquid path support is provided between the sampling device 1, the accommodating part 3 and the detection pipeline 23 through the liquid path support component 4 so as to obtain the detection result of hemoglobin and blood sedimentation together for the sample to be detected.

Specifically, the blood analyzer 100 may further include a control device 5, where the control device 5 may be electrically connected to the sampling device 1, the detection assembly, and the fluid path support assembly 4, respectively, to perform information processing and control and coordinate operations of the respective components. In addition, the blood analyzer 100 may further include a protein detection module or other detection modules for detecting a specific protein, which is not particularly limited herein, and other detection modules may be adaptively added according to actual detection requirements.

Specifically, the fluid circuit support assembly 4 may include functional support such as fluid actuation, reagent priming, fluid circuit cleaning, waste fluid drainage, and the like. For example, the liquid path support assembly 4 can provide cleaning liquid for the sampling device 1, the accommodating portion 3 and the detecting assembly, so as to clean the sampling device 1, the accommodating portion 3 and the detecting pipeline 23, respectively, to avoid polluting the blood sample to be detected and causing inaccurate detecting result. The liquid path support component 4 can also be connected with the sampling needle through a pipeline so as to keep the pipeline full of diluent, so that the processes of sampling, sample dividing and the like are realized more quickly and reliably. The fluid circuit support assembly 4 may be a pump, syringe or other source of power, such as a positive or negative air pressure source, or the like.

Specifically, the blood segment to be detected in the detection pipeline 23 may be directly flowed into the detection pipeline 23 via the sampling device 1 under the driving of the liquid pipeline support assembly 4, so that the first detection portion 21 can detect the blood segment to be detected in the detection pipeline 23. After the first detecting portion 21 detects the blood sedimentation, the sample to be detected in the detecting pipeline 23 can be transported into the accommodating portion 3 through the liquid path supporting component 4, and the sample to be detected is supplied to the accommodating portion 3 through the detecting pipeline 23, so that the hemoglobin of the sample to be detected in the accommodating portion 3 is detected through the second detecting portion 22. In this case, as an alternative embodiment, the test line 23 can be connected directly to the sampling needle of the sampling device 1, so that after the sample to be tested is pulled into the test line 23, the blood sedimentation of the sample to be tested can be detected directly by the first detection part 21.

In some embodiments, the detection line 23 is connected to a sampling needle of the sampling device 1. The method further comprises the steps of: drawing the sample to be tested collected by the sampling device 1 into the detection pipeline 23 through the liquid path supporting component 4; after the sample to be measured reaches the detection line 23, the blood sedimentation of the sample to be measured is detected via the first detection portion 21.

In some embodiments, the method further comprises: drawing the sample to be tested collected by the sampling device 1 into the accommodating part 3 through the liquid path supporting component 4, and drawing the sample to be tested in the accommodating part 3 into the detection pipeline 23; after the sample to be measured reaches the detection line 23 via the housing portion 3, the blood sedimentation of the sample to be measured is detected via the first detection portion 21.

In some embodiments, the method further comprises: after the first detecting unit 21 detects blood sedimentation, the blood segment to be measured in the detecting line 23 is discharged back into the accommodating unit 3 via the liquid path support unit 4, and a diluent is supplied into the accommodating unit 3.

In some embodiments, the method further comprises: after the diluent is supplied into the accommodating part 3, adding a hemolysis agent into the diluted sample to be tested through the liquid path supporting component 4 to form a detection sample capable of being used for detecting hemoglobin; after the formation of the test sample, hemoglobin of the test sample in the housing portion 3 is detected via the second detection portion 22.

In some embodiments, the detection assembly further comprises a third detection part 24 for detecting red blood cells, the third detection part 24 comprises a counting cell 241 having a jewel hole 242 with an electrode 243, and the counting cell 241 and the accommodating part 3 are respectively and independently arranged or integrally arranged.

In some embodiments, a sheath fluid inlet is provided on the counting cell 241 of the third detecting portion 24; the method further comprises the steps of: and the sheath liquid flowing in from the sheath liquid inlet wraps the sample to be detected and passes through the jewel hole 242 of the counting cell 241, so that the red blood cells of the sample to be detected are detected.

Specifically, the third detecting portion 24 may be further configured as a sheath flow impedance detecting portion, and a sheath liquid inlet may be provided on the counting cell 241 of the sheath flow impedance detecting portion, and a sheath liquid tank may be further provided for supplying sheath liquid to the counting cell 241. In the counting cell 241, the sample to be measured flows through the jewel hole 242 under the wrapping of the sheath liquid to change the sample to be measured into a thin stream, so that particles (formed components) contained in the sample to be measured pass through the jewel hole 242 one by one. Then, by detecting the impedance between the pair of electrodes 243, a resistance signal indicating a change in impedance is obtained, and the resistance signal is amplified by the amplifier 245 and sent to the control device 5. The magnitude of the resistance signal corresponds to the volume (size) of the particles, so that the red blood cell parameter and the platelet parameter of the sample to be measured can be obtained by signal processing of the resistance signal by the control device 5.

In some embodiments, the method further comprises: drawing the diluted blood segment to be measured into the counting pond 241 through the liquid path supporting component 4; the third detecting unit 24 detects the direct current impedance generated when the particles in the diluted sample to be measured pass through the jewel hole 242 of the counting cell 241, and outputs an electric signal reflecting information when the particles pass through the jewel hole 242, so as to detect the erythrocytes of the diluted sample to be measured.

In some embodiments, the method further comprises: after the third detecting unit 24 completes the detection of the red blood cells, adding a hemolytic agent to the diluted sample to be detected via the liquid path support member 4 to form a detection sample capable of being used for detecting hemoglobin; after the formation of the test sample, hemoglobin of the test sample is detected via the second detection unit 22.

Furthermore, although exemplary embodiments have been described herein, the scope thereof includes any and all embodiments having equivalent elements, modifications, omissions, combinations (e.g., of the various embodiments across schemes), adaptations or alterations based on the present disclosure. Elements in the claims are to be construed broadly based on the language employed in the claims and are not limited to examples described in the present specification or during the practice of the present application, which examples are to be construed as non-exclusive. It is intended, therefore, that the specification and examples be considered as exemplary only, with a true scope and spirit being indicated by the following claims and their full scope of equivalents.

The above description is intended to be illustrative and not restrictive. For example, the above-described examples (or one or more aspects thereof) may be used in combination with each other. For example, other embodiments may be used by those of ordinary skill in the art upon reading the above description. In addition, in the above detailed description, various features may be grouped together to streamline the disclosure. This is not to be interpreted as an intention that the disclosed features not being claimed are essential to any claim. Rather, the disclosed subject matter may include less than all of the features of a particular disclosed embodiment. Thus, the following claims are hereby incorporated into the detailed description as examples or embodiments, with each claim standing on its own as a separate embodiment, and it is contemplated that these embodiments may be combined with one another in various combinations or permutations. The scope of the disclosure should be determined with reference to the appended claims, along with the full scope of equivalents to which such claims are entitled.

The above embodiments are merely exemplary embodiments of the present disclosure, which are not intended to limit the present disclosure, the scope of which is defined by the claims. Various modifications and equivalent arrangements of parts may be made by those skilled in the art, which modifications and equivalents are intended to be within the spirit and scope of the present disclosure.

Claims

1. A blood analyzer, comprising:

a sampling device configured to collect a sample to be measured;

the detection assembly at least comprises a detection pipeline, a first detection part for detecting blood sedimentation and a second detection part for detecting hemoglobin, wherein the first detection part is arranged on the detection pipeline;

the accommodating part is configured to receive a sample to be detected in the sampling device and/or the detection pipeline, the detection pipeline is connected to the accommodating part, and the second detection part is sleeved outside the accommodating part;

and the liquid path support component is configured to provide liquid path support among the sampling device, the accommodating part and the detection pipeline so as to obtain the detection result of hemoglobin and blood sedimentation together for the sample to be detected.

2. The blood analyzer of claim 1, wherein the detection line is connected to a sampling needle of the sampling device;

The liquid path support assembly is further configured to: drawing the sample to be detected collected by the sampling device into the detection pipeline;

the first detection section is configured to: and detecting the blood sedimentation of the sample to be detected after the sample to be detected reaches the detection pipeline.

3. The blood analyzer of claim 1, wherein the fluid circuit support assembly is further configured to: drawing the sample to be detected collected by the sampling device into the accommodating part, and drawing the sample to be detected in the accommodating part into the detection pipeline;

the first detection section is configured to: and detecting the blood sedimentation of the sample to be detected after the sample to be detected reaches the detection pipeline through the accommodating part.

4. The blood analyzer of claim 1, wherein the fluid circuit support assembly is further configured to: after the first detection part completes the detection of blood sedimentation, the blood segment to be detected in the detection pipeline is spitted back into the accommodating part, and diluent is supplied into the accommodating part.

5. The blood analyzer of claim 4, wherein the fluid circuit support assembly is further configured to: after the diluent is supplied into the accommodating part, adding a hemolytic agent into the diluted sample to be tested to form a detection sample capable of being used for detecting hemoglobin;

The second detection section is configured to: and detecting hemoglobin of the detection sample in the accommodating portion after the detection sample is formed.

6. The blood analyzer of claim 4, wherein the detection assembly further comprises a third detection section for detecting red blood cells, the third detection section comprising a counting cell having a jewel orifice with an electrode, the counting cell being disposed independently of or integrally with the receiving section.

7. The blood analyzer according to claim 6, wherein a sheath fluid inlet is provided in the counting cell of the third detecting portion to wrap a sample to be measured through a jewel hole of the counting cell by sheath fluid flowing in from the sheath fluid inlet, thereby detecting red blood cells of the sample to be measured.

8. The blood analyzer of claim 6 or 7, wherein the fluid circuit support assembly is further configured to: drawing the diluted blood segment to be measured into the counting pool;

the third detection section is configured to: and detecting direct current impedance generated when particles in the diluted sample to be detected pass through the jewel hole of the counting cell, and outputting an electric signal reflecting information when the particles pass through the jewel hole so as to detect red blood cells of the diluted sample to be detected.

9. The blood analyzer of claim 8, wherein the fluid circuit support assembly is further configured to: after the third detection part completes the detection of the red blood cells, adding a hemolytic agent into the diluted sample to be detected to form a detection sample capable of being used for detecting the hemoglobin;

the second detection section is configured to: after the test sample is formed, hemoglobin of the test sample is detected.

10. A blood detection method is characterized by being applied to a blood analyzer, wherein the blood analyzer comprises a sampling device, a containing part, a detection component and a liquid path support component, the detection component at least comprises a detection pipeline, a first detection part for detecting blood sedimentation and a second detection part for detecting hemoglobin, the first detection part is arranged on the detection pipeline,

the blood detection method comprises the following steps:

collecting a sample to be tested via the sampling device;

receiving a sample to be tested from the sampling device and/or a detection pipeline through the accommodating part, wherein the detection pipeline is connected to the accommodating part, and the second detection part is sleeved outside the accommodating part;

and providing liquid path support among the sampling device, the accommodating part and the detection pipeline through the liquid path support component so as to obtain the detection result of hemoglobin and blood sedimentation together for the sample to be detected.

11. The blood testing method of claim 10, wherein the testing tubing is connected to a sampling needle of the sampling device;

the blood detection method further comprises:

drawing the sample to be detected collected by the sampling device into the detection pipeline through the liquid path supporting component;

and detecting the blood sedimentation of the sample to be detected through the first detection part after the sample to be detected reaches the detection pipeline.

12. The blood testing method of claim 10, further comprising:

drawing the sample to be tested collected by the sampling device into the accommodating part through the liquid path supporting component, and drawing the sample to be tested in the accommodating part into the detection pipeline;

after the sample to be tested reaches the detection pipeline through the accommodating part, blood sedimentation of the sample to be tested is detected through the first detection part.

13. The blood testing method of claim 10, further comprising:

after the first detection part completes the detection of blood sedimentation, the blood segment to be detected in the detection pipeline is spitted back into the accommodating part through the liquid path supporting component, and diluent is supplied into the accommodating part.

14. The blood testing method of claim 13, further comprising:

after the diluent is supplied into the accommodating part, adding a hemolysis agent into the diluted sample to be tested through the liquid path supporting component so as to form a detection sample capable of being used for detecting hemoglobin;

after the formation of the test sample, hemoglobin of the test sample in the housing portion is detected via the second detection portion.

15. The method of claim 13, wherein the testing assembly further comprises a third testing portion for testing red blood cells, the third testing portion comprising a counting cell having a jewel orifice with electrodes, the counting cell being disposed independently of or integral with the receiving portion.

16. The method according to claim 15, wherein a sheath fluid inlet is provided in the counting cell of the third detecting portion;

the blood detection method further comprises: and the sheath liquid flowing in from the sheath liquid inlet wraps the sample to be detected and passes through the jewel hole of the counting cell, so that the red blood cells of the sample to be detected are detected.

17. The blood test method of claim 15 or 16, wherein the blood test method further comprises:

Drawing the diluted blood segment to be measured into the counting pool through the liquid path supporting component;

and detecting direct current impedance generated when particles in the diluted sample to be detected pass through the jewel hole of the counting cell through the third detection part, and outputting an electric signal reflecting information when the particles pass through the jewel hole so as to detect red blood cells of the diluted sample to be detected.

18. The blood testing method of claim 17, further comprising:

after the third detection part completes the detection of the red blood cells, adding a hemolytic agent into the diluted sample to be detected through the liquid path supporting component so as to form a detection sample capable of being used for detecting the hemoglobin;

after the formation of the test sample, hemoglobin of the test sample is detected via the second detection section.