CN116067846B - Method for testing mechanical strength of aerobic granular sludge - Google Patents
Method for testing mechanical strength of aerobic granular sludge Download PDFInfo
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- 239000010802 sludge Substances 0.000 title claims abstract description 58
- 238000012360 testing method Methods 0.000 title claims abstract description 43
- 238000000034 method Methods 0.000 title claims abstract description 26
- 239000002245 particle Substances 0.000 claims abstract description 55
- 230000008859 change Effects 0.000 claims abstract description 41
- 230000009471 action Effects 0.000 claims abstract description 20
- 238000010008 shearing Methods 0.000 claims abstract description 9
- 230000036962 time dependent Effects 0.000 claims abstract description 5
- 238000010586 diagram Methods 0.000 claims abstract description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical group O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 4
- 239000008367 deionised water Substances 0.000 claims description 2
- 229910021641 deionized water Inorganic materials 0.000 claims description 2
- 239000002270 dispersing agent Substances 0.000 claims description 2
- 230000009286 beneficial effect Effects 0.000 abstract description 5
- 238000004458 analytical method Methods 0.000 abstract description 4
- 238000012258 culturing Methods 0.000 abstract description 4
- 238000012512 characterization method Methods 0.000 abstract description 3
- 238000000605 extraction Methods 0.000 abstract 1
- 238000011002 quantification Methods 0.000 abstract 1
- 239000002028 Biomass Substances 0.000 description 3
- 239000012528 membrane Substances 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 239000008187 granular material Substances 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 238000005273 aeration Methods 0.000 description 1
- 238000005054 agglomeration Methods 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 229910001424 calcium ion Inorganic materials 0.000 description 1
- 230000006835 compression Effects 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- 238000005336 cracking Methods 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 239000010865 sewage Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
- 238000004065 wastewater treatment Methods 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/02—Investigating particle size or size distribution
- G01N15/0205—Investigating particle size or size distribution by optical means
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
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- Chemical & Material Sciences (AREA)
- Dispersion Chemistry (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
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- Immunology (AREA)
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- Treatment Of Sludge (AREA)
- Activated Sludge Processes (AREA)
Abstract
The invention discloses a method for testing the mechanical strength of aerobic granular sludge, which comprises the following steps: shearing aerobic granular sludge samples at different rotating speeds by using a laser particle size analyzer, and removing points with larger deviation by using a Dv10/50/90 time-dependent change chart; drawing a particle size distribution change chart under the action time of equal intervals; analysis and extraction of the rate of change of peak area and the like by softwareOr the variation delta S information, drawing the action time as the abscissa,or DeltaS is a scatter diagram with an ordinate, a curve is fitted, and the mechanical strength is characterized by quantification of the curve. Wherein, the smaller the change rate (or the change amount) of the peak area, the better the mechanical strength of the aerobic granular sludge is. The invention can realize the quantitative characterization and the standardized test of the mechanical strength of the aerobic granular sludge simply and accurately, forms a standardized data system, is beneficial to the comparison of the mechanical strength of the aerobic granular sludge formed by culturing different reactors, and provides a beneficial reference for further improving the stable operation of the reactors.
Description
Technical Field
The invention belongs to the technical field of biological wastewater treatment, and particularly relates to a method for testing the mechanical strength of aerobic granular sludge.
Background
The aerobic granular sludge (Aerobic Granular Sludge, AGS) is a granular activated sludge formed by self-agglomeration of microorganisms under aerobic conditions. Compared with the traditional activated sludge process, the AGS technology has the characteristics of high biomass accumulation, good sedimentation performance, pollution load impact resistance, small occupied area and the like.
Heretofore, aerobic granular sludge bioreactors include Membrane Bioreactors (MBR), sequencing Batch Reactors (SBR), and the like. However, the success of these bioreactor designs is related to their ability to form dense particles and maintain high biomass. The close cell packing in the particle aggregates optimizes the interplanar exchange of metabolites, thereby increasing overall activity.
However, from the reactor operating conditions, too high/low Organic Loading (OLR) and too high hydrodynamic conditions negatively affect the particles, possibly leading to cracking and dispersion, a significant reduction of biomass, and eventually a deterioration or even failure of the system. From the operation time, AGS is easily disintegrated after long-term operation. Therefore, maintaining the stability of AGS is critical for the stable operation of the reactor, while its mechanical properties are important indicators for characterizing its stability. In general, the mechanical properties of AGS are divided into compression and shear properties. The existing compressive property testing method is a measuring device and a measuring method for the elastic modulus of granular sludge, which are disclosed in Chinese patent CN 104062224A. The method for testing the shearing resistance comprises an integrity coefficient method (a vibrating screen is adopted and is judged by measuring the ratio of the solid mass in supernatant to the total weight of aerobic granular sludge), a instability coefficient method (an ultrasonic instrument is adopted, samples collected at equal intervals are subjected to absorbance value test at the wavelength of 600nm, and the slope of a fitted curve is the instability coefficient) and the like. Therefore, the existing test method has the advantage of rapid measurement, but the collected sample needs to be poured back in time in the test to prevent the volume change from influencing the experimental result, so that the establishment of a method for simultaneously satisfying the simple and accurate measurement of the mechanical strength is particularly important.
Disclosure of Invention
The invention provides a method for testing the mechanical strength of the aerobic granular sludge, which can realize the quantitative characterization and the standardized test of the mechanical strength of the aerobic granular sludge simply, conveniently and accurately.
In order to solve the technical problems, the invention adopts the following technical scheme: a method for testing the mechanical strength of aerobic granular sludge, comprising the following steps:
s1, shearing aerobic granular sludge samples at different rotating speeds by using a laser particle size analyzer, and removing points with larger deviation by using a Dv10/50/90 time-dependent change chart;
s2, drawing a particle size distribution change chart under the action time t with equal intervals;
s3, analyzing and extracting the peak area change rate by utilizing software in the step S2Or information of the variation DeltaS, and drawing the peak area variation rate about the action time as the abscissa>Or a scatter diagram with the variation delta S as an ordinate, fitting a curve, and quantitatively representing the mechanical strength of the aerobic granular sludge through the curve; the smaller the peak area change rate or the change amount is, the smaller the particle diameter change of the particles in the shearing process is, and the better the mechanical strength of the aerobic granular sludge is.
Wherein S is the area of a region surrounded by the particle size distribution and the x axis, namely the peak area; t is the duration of action of the particle size distribution test.
According to the testing method provided by the invention, the shear resistance is used for representing the mechanical properties of the AGS, the mechanical strength of the aerobic granular sludge is quantitatively represented through the change rate or the change quantity of the particle size distribution peak area and the like, the mechanical strength comparison of the aerobic granular sludge formed by culturing different reactors is facilitated, the basis is provided for establishing the response mechanism of the reactor operation elements and the mechanical strength of the granules, and the optimal scheme is provided for culturing the aerobic granular sludge with good strength, so that the testing method has important practical significance and economic value.
Wherein, the aerobic granular sludge is not limited by the bioreactor for culture; the mechanical strength of the aerobic granular sludge may also be referred to as shear resistance, structural stability or mechanical stability.
Further, in the step S3, the peak height change amount Δh or the change rate can be usedCharacterizing the mechanical strength of the aerobic granular sludge; where h is the shortest distance between the x-axis and the peak top point, i.e., the peak height.
Further, the change amount Δd or the change rate of the peak apex can be usedCharacterizing the mechanical strength of aerobic granular sludge, wherein d is two adjacent peaksDistance between vertices.
Further, the mechanical strength of the aerobic granular sludge can be characterized by a limiting peak particle size R, wherein R is the peak particle size, and the limiting peak particle size is particularly shown here and is obtained by a Dv10/50/90 time-dependent graph.
Further, in step S1, the selected aerobic granular sludge is generally an existing sample, suspended flocculent sludge is removed through a screen, and then a vortex mixer is used for uniform mixing, so as to ensure the accuracy of the test.
Further, in the step S2, the abscissa is the particle size of the aerobic granular sludge, and the ordinate is the capacity ratio.
Further, in the step S1, the particle size of the aerobic granular sludge before shearing is 200-3000 μm. Preferably 500 μm to 2500. Mu.m.
Further, in the step S1, the rotation speed omega is 500r/min-3500r/min.
Further, in the step S1, the action time is 15-90 min; and testing at equal intervals for 1.5-5 min. The preferable action time is 30 min-60 min; the test is carried out at equal intervals of 1.5min to 2.5 min.
Further, in the step S1, the laser particle size analyzer is Malvern MS3000, and the parameters are set as follows: the dispersing agent is deionized water; the refractive index is 1-2; the absorptivity is 0.05-0.2; the shading degree is 5-50.
Further, the test result needs to meet that RSD values of Dv10, dv50 and Dv90 are respectively smaller than 10, 6 and 10, and fitting weight is smaller than 2.5%; where Dv10 means that 10% of the sample particle size is smaller than this value, dv50 means that 50% of the sample particle size is smaller than this value, and Dv90 means that 90% of the sample particle size is smaller than this value.
Compared with the prior art, the beneficial effects are that:
1. the invention adopts a laser particle size analyzer which is a commonly used instrument for measuring particle size, and has the characteristics of rapidness, accuracy, high degree of automation, less influence by concentration and external factors and the like.
2. According to the testing method, the mechanical strength of the aerobic granular sludge is represented by the equivalent of the variation/variation rate of the peak area or peak height, and the granules generated by different bioreactors are tested and compared at different rotating speeds, so that the testing method has universality and practicability. The invention can realize the quantitative characterization and the standardized test of the mechanical strength of the aerobic granular sludge simply and accurately, forms a standardized data system, is beneficial to the comparison of the mechanical strength of the aerobic granular sludge formed by culturing different reactors, and provides a beneficial reference for further improving the stable operation of the reactors.
Drawings
FIG. 1 is a flow chart of the method for testing the mechanical strength of aerobic granular sludge.
FIG. 2 is a graph showing the change in particle size distribution at equal intervals in the examples.
FIG. 3 is a graph of a fit of the peak area change rate over time for the examples.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and fully with reference to the accompanying drawings, in which it is evident that the embodiments described are only some, but not all embodiments of the invention. The invention is described in one of its examples in connection with the following detailed description. Wherein the drawings are for illustrative purposes only and are shown in schematic, non-physical, and not intended to be limiting of the present patent; for the purpose of better illustrating embodiments of the invention, certain elements of the drawings may be omitted, enlarged or reduced and do not represent the size of the actual product; it will be appreciated by those skilled in the art that certain well-known structures in the drawings and descriptions thereof may be omitted.
Example 1:
the present invention will be described in further detail with reference to the following specific examples and drawings, but the embodiments of the present invention are not limited thereto. The apparatus employed in the present invention is conventional in the art unless otherwise specified.
And (3) testing the mechanical strength of the aerobic granular sludge with different particle sizes (large and small) in the reactor 1 at the rotating speed of 2250 r/min.
A method for testing the mechanical strength of aerobic granular sludge, comprising the following steps:
s1, shearing aerobic granular sludge samples at different rotating speeds by using a laser particle size analyzer, and removing points with larger deviation by using a Dv10/50/90 time-dependent change chart;
s2, drawing a particle size distribution change chart under the action time t with equal intervals, wherein the abscissa is the particle size (mum) of particles, and the ordinate is the capacity ratio (%);
s3, analyzing and extracting the peak area change rate by utilizing software in the step S2Or information of the variation DeltaS, and drawing the peak area variation rate about the action time as the abscissa>Or a scatter diagram with the variation delta S as an ordinate, fitting a curve, and quantitatively representing the mechanical strength of the aerobic granular sludge through the curve; the smaller the peak area change rate or the change amount is, the smaller the particle diameter change of the particles in the shearing process is, and the better the mechanical strength of the aerobic granular sludge is.
Wherein S is the area of a region surrounded by the particle size distribution and the x axis, namely the peak area; t is the duration of action of the particle size distribution test.
In the embodiment, in the step S1, aerobic granular sludge comes from a rectangular membrane bioreactor 1 (35 multiplied by 25 multiplied by 30 cm), and a plastic net is adopted to separate an aerobic zone and a membrane assembly zone with a volume ratio of 2:1; the water inlet is artificial synthetic wastewater composed of sucrose (392.7 mg/L) and other nutrient substances; the cultured particles take filamentous microorganisms as frameworks, are ellipsoidal, have smooth surfaces and are mainly brown; the sample is divided into two different particle sizes; setting the rotating speed to 2250r/min, setting the acting time to 50min, and testing at equal intervals for 2 min; the shading degree of the laser particle size analyzer is set to be 10-20, the test result is shown in fig. 2, and a fitting curve chart of the change rate of the peak area, which is obtained by analysis in the step S3, along with the change of the action time is shown in fig. 3-a;
comparing the fitted curves, the peak area change rate of the small particle size is smaller than that of the large particle size, which shows that the mechanical strength of the small particle size AGS is stronger.
Example 2
Mechanical Strength test of aerobic granular sludge in reactor 2 at 2250r/min
This embodiment differs from embodiment 1 in that: the AGS used is different, the particles used in the embodiment come from a reactor 2 (4750 multiplied by 2500 multiplied by 3580 mm), and consist of an aeration area and a mixing area, the inlet water is domestic sewage, and the cultured particles mainly take calcium ions as a framework, and have lighter internal color and darker external color; the experimental test of different gradient particle sizes is not set, the action time is set to be 32min, the test result is shown in figure 2, and the fitting curve graph of the change rate of the peak area, which is obtained by analysis in step S3, along with the change of the action time is shown in figure 3-a; by comparison with the fitted curve of example 1, it can be seen that the AGS formed in reactor 2 has the greatest peak area change rate over a shorter duration of action, indicating that the mechanical strength of the formed AGS is the weakest.
Example 3
Mechanical strength test of aerobic granular sludge in reactor 1 at rotational speeds of 1500, 2250, 3000r/min
This embodiment differs from embodiment 1 in that: setting different gradient rotating speed experimental tests, but not setting different gradient particle size experimental tests, wherein the test result is shown in fig. 2, and the fitting curve graph of the peak area change rate obtained by analysis in step S3 along with the change of the action time is shown in fig. 3-b; by comparing the fitted curves, the peak area change rate of the AGS formed by the reactor 1 is increased along with the increase of the rotating speed under the condition of shorter and same action time, which shows that the mechanical strength of the AGS is reduced along with the increase of the rotating speed within the range of 1500-3000 r/min.
It is to be understood that the above examples of the present invention are provided by way of illustration only and not by way of limitation of the embodiments of the present invention. Other variations or modifications of the above teachings will be apparent to those of ordinary skill in the art. It is not necessary here nor is it exhaustive of all embodiments. Any modification, equivalent replacement, improvement, etc. which come within the spirit and principles of the invention are desired to be protected by the following claims.
Claims (10)
1. A method for testing the mechanical strength of aerobic granular sludge, which is characterized by comprising the following steps:
s1, shearing aerobic granular sludge samples at different rotating speeds by using a laser particle size analyzer, and removing points with larger deviation by using a Dv10/50/90 time-dependent change chart;
s2, drawing a particle size distribution change chart under the action time t with equal intervals;
s3, analyzing and extracting the peak area change rate by using software in the step S2Or variation->The information of (2) is plotted with the time of action as abscissa, the peak area change rate +.>Or variation->Fitting a curve for a scatter diagram with an ordinate, and quantitatively representing the mechanical strength of the aerobic granular sludge through the curve; the smaller the peak area change rate or the change amount is, the better the mechanical strength of the aerobic granular sludge is;
wherein S is the area of a region surrounded by the particle size distribution and the x axis, namely the peak area; t is the duration of action of the particle size distribution test.
2. The method for testing mechanical strength of aerobic granular sludge according to claim 1, wherein in said step S3, the variation of peak height is also passedOr rate of change->Characterizing the mechanical strength of the aerobic granular sludge; where h is the shortest distance between the x-axis and the peak top point, i.e., the peak height.
3. The method for testing the mechanical strength of aerobic granular sludge according to claim 2, wherein the variation of the peak-to-peak point can be usedOr rate of change->And (3) representing the mechanical strength of the aerobic granular sludge, wherein d is the distance between two adjacent peak top points.
4. A method for testing the mechanical strength of aerobic granular sludge according to claim 3, characterized in that the mechanical strength of the aerobic granular sludge can be further characterized by a limiting peak particle size R, wherein the limiting peak particle size is obtained by Dv10/50/90 as a function of time.
5. The method according to claim 4, wherein in the step S2, the abscissa represents the particle size of the aerobic granular sludge and the ordinate represents the capacity ratio.
6. The method according to any one of claims 1 to 5, wherein in the step S1, the particle size of the aerobic granular sludge before shearing is 200 μm to 3000 μm.
7. The method according to any one of claims 1 to 5, wherein in the step S1, the rotation speed ω is 500r/min-3500r/min.
8. The method according to any one of claims 1 to 5, wherein in the step S1, the action time is 15min to 90min; and testing at equal intervals for 1.5-5 min.
9. The method according to any one of claims 1 to 5, wherein in the step S1, the laser particle size analyzer is Malvern MS3000, and the parameters are set as follows: the dispersing agent is deionized water; the refractive index is 1-2; the absorptivity is 0.05-0.2; the shading degree is 5-50.
10. The method for testing the mechanical strength of the aerobic granular sludge according to claim 9, wherein the test result is required to meet the RSD values of Dv10, dv50 and Dv90 of < 10, 6 and 10 respectively, and the fitting weight is less than 2.5%; where Dv10 means that 10% of the sample particle size is smaller than this value, dv50 means that 50% of the sample particle size is smaller than this value, and Dv90 means that 90% of the sample particle size is smaller than this value.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103196781A (en) * | 2013-04-15 | 2013-07-10 | 杭州师范大学 | Measuring method and device for mechanical strength of granular sludge |
| CN103776780A (en) * | 2014-01-28 | 2014-05-07 | 杭州师范大学 | Method for detecting strength of granular sludge |
| MY168903A (en) * | 2009-02-20 | 2018-12-04 | Univ Malaysia Teknologi | Procedure development for evaluation of aerobic granular sludge physical strength |
| CN113479998A (en) * | 2021-04-27 | 2021-10-08 | 合肥工业大学 | Comprehensive evaluation method for aerobic sludge granulation degree |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MY168903A (en) * | 2009-02-20 | 2018-12-04 | Univ Malaysia Teknologi | Procedure development for evaluation of aerobic granular sludge physical strength |
| CN103196781A (en) * | 2013-04-15 | 2013-07-10 | 杭州师范大学 | Measuring method and device for mechanical strength of granular sludge |
| CN103776780A (en) * | 2014-01-28 | 2014-05-07 | 杭州师范大学 | Method for detecting strength of granular sludge |
| CN113479998A (en) * | 2021-04-27 | 2021-10-08 | 合肥工业大学 | Comprehensive evaluation method for aerobic sludge granulation degree |
Non-Patent Citations (1)
| Title |
|---|
| Strength characterization of full-scale aerobic granular sludge;Danny R. de Graaff, et al;Environmental Technology;第41卷(第13期);1637-1647 * |
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