CN103776780A - Method for detecting strength of granular sludge - Google Patents
Method for detecting strength of granular sludge Download PDFInfo
- Publication number
- CN103776780A CN103776780A CN201410042419.3A CN201410042419A CN103776780A CN 103776780 A CN103776780 A CN 103776780A CN 201410042419 A CN201410042419 A CN 201410042419A CN 103776780 A CN103776780 A CN 103776780A
- Authority
- CN
- China
- Prior art keywords
- ultrasonic
- granular sludge
- sludge
- absorbance
- ultrasonic instrument
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000010802 sludge Substances 0.000 title claims abstract description 49
- 238000000034 method Methods 0.000 title claims abstract description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 12
- 239000012153 distilled water Substances 0.000 claims abstract description 8
- 238000005070 sampling Methods 0.000 claims abstract description 8
- 238000002835 absorbance Methods 0.000 claims abstract description 7
- 238000001514 detection method Methods 0.000 claims 1
- 230000000875 corresponding effect Effects 0.000 description 5
- 239000002245 particle Substances 0.000 description 4
- 238000005259 measurement Methods 0.000 description 3
- 238000002604 ultrasonography Methods 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 2
- 238000011481 absorbance measurement Methods 0.000 description 2
- 238000012512 characterization method Methods 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- 239000002028 Biomass Substances 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 238000004065 wastewater treatment Methods 0.000 description 1
Images
Landscapes
- Investigating Or Analyzing Materials By The Use Of Ultrasonic Waves (AREA)
Abstract
本发明提供了一种颗粒污泥强度的检测方法，所述方法为：取湿重为4.8～5.2g的待测颗粒污泥，用蒸馏水清洗，清洗后的污泥置于100mL烧杯内，加蒸馏水定容至50mL；将装有污泥的烧杯固定于超声仪中，水温控制在24～26℃；设置超声频率为20kHz，超声功率为65W，开启超声仪，对颗粒污泥进行超声操作；从开启超声仪开始，每隔1min取样3mL；取样10-12次后，关闭超声仪，将不同时间点取得的样品用可见分光光度计在600nm波长下，分别测定吸光度；以取样时间为横坐标，吸光度为纵坐标，绘制吸光度-时间曲线，得出斜率即为失稳系数；失稳系数越大，表明颗粒污泥的机械强度越小。本发明具有泥损少、易于操作、适用面广、测试成本低等优点。The invention provides a method for detecting the intensity of granular sludge. The method is as follows: take the granular sludge to be tested with a wet weight of 4.8 to 5.2 g, wash it with distilled water, place the cleaned sludge in a 100mL beaker, add Distilled water was adjusted to 50mL; the beaker containing the sludge was fixed in the ultrasonic instrument, and the water temperature was controlled at 24-26°C; the ultrasonic frequency was set to 20kHz, the ultrasonic power was 65W, the ultrasonic instrument was turned on, and the granular sludge was ultrasonically operated; Starting from turning on the ultrasonic instrument, sample 3mL every 1min; after sampling 10-12 times, turn off the ultrasonic instrument, and measure the absorbance of the samples obtained at different time points with a visible spectrophotometer at a wavelength of 600nm; take the sampling time as the abscissa , the absorbance is the ordinate, draw the absorbance-time curve, and the slope is the instability coefficient; the larger the instability coefficient, the smaller the mechanical strength of the granular sludge. The invention has the advantages of less mud loss, easy operation, wide applicability and low test cost.
Description
技术领域technical field
本发明涉及一种颗粒污泥强度的检测方法。The invention relates to a method for detecting the strength of granular sludge.
背景技术Background technique
在废水处理领域，与絮状污泥相比，颗粒污泥具有外形规则、密度大、强度高、结构稳定、沉降性能突出等优点。采用颗粒污泥的反应器可以保有较高的生物量和生物活性。机械强度是颗粒污泥的重要性质，在生物反应器中，较好的颗粒污泥强度是维持颗粒的稳定性和实现固液分离的必要条件。若只对颗粒强度进行定性描述不足以满足科研和生产所需，定量表征是必然要求。然而，迄今为止，测定颗粒污泥强度的方法过于复杂，操作过程易受外界条件影响，不利于对其进行准确表征。In the field of wastewater treatment, compared with flocculent sludge, granular sludge has the advantages of regular shape, high density, high strength, stable structure, and outstanding settling performance. Reactors using granular sludge can maintain high biomass and biological activity. Mechanical strength is an important property of granular sludge. In a bioreactor, better granular sludge strength is a necessary condition for maintaining the stability of the particles and achieving solid-liquid separation. If only a qualitative description of particle strength is not enough to meet the needs of scientific research and production, quantitative characterization is an inevitable requirement. However, to date, the methods for determining the strength of granular sludge are too complicated, and the operation process is easily affected by external conditions, which is not conducive to its accurate characterization.
针对这些问题，本发明提出一种用超声-可见分光光度简易测定颗粒污泥强度的方法。根据超声波空化效应，使颗粒污泥发生一定程度的破碎，破碎程度和污泥的强度呈负相关，通过吸光度测定得出相对应的吸光度-时间关系曲线，得出失稳系数，比较颗粒污泥强度，操作方法简单，测试成本低。In view of these problems, the present invention proposes a method for simply measuring the strength of granular sludge by ultrasonic-visible spectrophotometry. According to the ultrasonic cavitation effect, the granular sludge is broken to a certain extent, and the degree of brokenness is negatively correlated with the strength of the sludge. The corresponding absorbance-time relationship curve is obtained by absorbance measurement, and the instability coefficient is obtained. Mud strength, simple operation method, low test cost.
发明内容Contents of the invention
本发明的目的是提供一种颗粒污泥强度的检测方法。The purpose of the present invention is to provide a method for detecting the intensity of granular sludge.
本发明采用的技术方案是：The technical scheme adopted in the present invention is:
一种颗粒污泥强度的检测方法，所述方法包括以下步骤：A method for detecting granular sludge strength, said method comprising the following steps:
（1）取湿重为4.8～5.2g（优选4.9～5.1g）的待测颗粒污泥，用蒸馏水清洗3-5次，洗去颗粒污泥表面吸附的杂质，清洗后的污泥置于100mL烧杯内，加蒸馏水定容至50mL；将装有污泥的烧杯固定于超声仪中，水温控制在24～26℃；设置超声频率为20kHz，超声功率为65W，开启超声仪，对颗粒污泥进行超声操作；从开启超声仪开始，每隔1min取样3mL；取样10-12次后，关闭超声仪，将不同时间点取得的样品用可见分光光度计在600nm波长下，分别测定吸光度；(1) Take the granular sludge to be tested with a wet weight of 4.8-5.2g (preferably 4.9-5.1g), wash it with distilled water for 3-5 times, wash away the impurities adsorbed on the surface of the granular sludge, and place the cleaned sludge in In a 100mL beaker, add distilled water to make the volume to 50mL; fix the beaker with sludge in the ultrasonic instrument, and control the water temperature at 24-26°C; set the ultrasonic frequency to 20kHz, the ultrasonic power to 65W, turn on the ultrasonic instrument, The mud was subjected to ultrasonic operation; from the start of the ultrasonic instrument, 3 mL was sampled every 1 min; after sampling 10-12 times, the ultrasonic instrument was turned off, and the absorbance of the samples obtained at different time points was measured with a visible spectrophotometer at a wavelength of 600 nm;
（2）以取样时间为横坐标，相应时间的吸光度为纵坐标，绘制吸光度-时间曲线，计算线性方程，得出相对应的斜率即为失稳系数；失稳系数越大，表明颗粒污泥容易被超声波破坏，颗粒污泥的机械强度越小。(2) Take the sampling time as the abscissa, and the absorbance at the corresponding time as the ordinate, draw the absorbance-time curve, calculate the linear equation, and obtain the corresponding slope as the instability coefficient; the larger the instability coefficient, the greater the granular sludge The easier it is to be destroyed by ultrasonic waves, the smaller the mechanical strength of granular sludge.
所用仪器：超声设备为额定频率28kHz超声仪（华深科工，深圳），超声仪功率为0-800W。吸光度测定设备为可见分光光度计。Instruments used: Ultrasonic equipment is a rated frequency 28kHz ultrasonic instrument (Hua Shen Science and Technology, Shenzhen), and the power of the ultrasonic instrument is 0-800W. The absorbance measurement device is a visible spectrophotometer.
所述超声优选采用10s开-10s关的间歇超声的方式。The ultrasound is preferably in the form of intermittent ultrasound with 10s on-10s off.
本发明专利的优点主要体现在：1）测定过程所需泥量较少，可节约污泥颗粒；2）测定方法可操作性强，测定参数易于控制，测定数据处理简便，结果简单明了；3）测定方法适用范围广，可测定多种污泥颗粒；4）测试成本低。The advantages of the patent of the present invention are mainly reflected in: 1) The amount of mud required for the measurement process is small, which can save sludge particles; 2) The measurement method is highly operable, the measurement parameters are easy to control, the measurement data is easy to process, and the results are simple and clear; 3 ) The determination method has a wide range of applications and can measure a variety of sludge particles; 4) The test cost is low.
附图说明Description of drawings
图1颗粒污泥R1的吸光度-时间曲线图。Figure 1 Absorbance-time curve of granular sludge R1.
图2颗粒污泥R2的吸光度-时间曲线图。Figure 2 Absorbance-time curve of granular sludge R2.
具体实施方式Detailed ways
下面结合具体实施例子对本发明进行进一步描述，但本发明的保护范围并不仅限于此：The present invention is further described below in conjunction with specific implementation examples, but protection scope of the present invention is not limited thereto:
实施例1：本实施例采用本发明所述的测定方法对厌氧氨氧化颗粒污泥的强度进行了测定，具体如下：Embodiment 1: the present embodiment adopts the measuring method of the present invention to measure the intensity of the anammox granular sludge, specifically as follows:
1）取一升流式厌氧污泥床反应器中混匀的湿重为4.9772g的厌氧氨氧化颗粒污泥，记为R1，从另一反应器中取混匀的湿重为5.1083g厌氧氨氧化颗粒污泥，记为R2，各用蒸馏水清洗5次，洗去颗粒污泥表面吸附的杂质；2）将上述颗粒污泥分别置于2个对应的100mL烧杯内，分别加蒸馏水定容至50mL；3）将装有待测颗粒污泥的烧杯固定于超声仪的中央，水温控制在25±1℃；4）设置超声频率为20kHz，超声功率为65W，开启超声仪，采用10s开-10s关间歇超声的方式，对颗粒污泥进行超声操作；5）从开启超声仪开始，每隔1min取样3mL；6）取样10次后，关闭超声仪，将不同时间取得的样品用可见分光光度计在600nm波长下，分别测定吸光度。7)记录数据，如表1所示，绘制吸光度-时间曲线，颗粒污泥R1的吸光度-时间曲线图见图1，颗粒污泥R1的吸光度-时间曲线图见图2，得出相应的斜率即失稳系数，表征颗粒污泥强度；由图1、2可知，颗粒污泥R1的失稳系数k1为0.0176，颗粒污泥R2的k2为0.0269。从测试结果可以看出：k1＜k2，说明第一个反应器中颗粒污泥R1的机械强度大于第二个反应器中颗粒污泥R2的机械强度。1) Take the anaerobic ammonium oxidation granular sludge with a wet weight of 4.9772g mixed in a liter flow anaerobic sludge bed reactor, denoted as R1, and take the mixed wet weight from another reactor with a wet weight of 5.1083 g anaerobic ammonium oxidation granular sludge, denoted as R2, washed with distilled water 5 times each to remove impurities adsorbed on the surface of the granular sludge; 2) Put the above granular sludge into two corresponding 100mL beakers, add Distilled water to 50mL; 3) Fix the beaker containing the granular sludge to be tested in the center of the ultrasonic instrument, and control the water temperature at 25±1°C; 4) Set the ultrasonic frequency to 20kHz, the ultrasonic power to 65W, turn on the ultrasonic instrument, Ultrasonic operation was performed on the granular sludge by means of 10s on-10s off intermittent ultrasound; 5) Starting from the start of the ultrasonic instrument, sampling 3mL every 1min; 6) After sampling 10 times, turn off the ultrasonic instrument, and the Absorbance was measured with a visible spectrophotometer at a wavelength of 600 nm. 7) Record the data, as shown in Table 1, draw the absorbance-time curve, the absorbance-time curve of granular sludge R1 is shown in Figure 1, the absorbance-time curve of granular sludge R1 is shown in Figure 2, and the corresponding slope is obtained That is, the instability coefficient, which characterizes the strength of granular sludge; it can be seen from Figures 1 and 2 that the instability coefficient k 1 of granular sludge R1 is 0.0176, and the k 2 of granular sludge R2 is 0.0269. It can be seen from the test results that k1<k2 indicates that the mechanical strength of the granular sludge R1 in the first reactor is greater than that of the granular sludge R2 in the second reactor.
表1 数据记录表Table 1 Data Recording Table
本说明书实施例所述的内容仅仅是对发明构思的实现形式的列举，本发明的保护范围不应当被视为仅限于实施例所陈述的具体形式，本发明的保护范围也及于本领域技术人员根据本发明构思所能够想到的等同技术手段。The content described in the embodiments of this specification is only an enumeration of the implementation forms of the inventive concept. The protection scope of the present invention should not be regarded as limited to the specific forms stated in the embodiments. Equivalent technical means that a person can think of based on the concept of the present invention.
Claims (2)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410042419.3A CN103776780B (en) | 2014-01-28 | 2014-01-28 | Method for detecting strength of granular sludge |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410042419.3A CN103776780B (en) | 2014-01-28 | 2014-01-28 | Method for detecting strength of granular sludge |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103776780A true CN103776780A (en) | 2014-05-07 |
| CN103776780B CN103776780B (en) | 2017-04-19 |
Family
ID=50569284
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410042419.3A Active CN103776780B (en) | 2014-01-28 | 2014-01-28 | Method for detecting strength of granular sludge |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103776780B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116067846A (en) * | 2022-12-15 | 2023-05-05 | 广东工业大学 | A method for testing the mechanical strength of aerobic granular sludge |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5624099A (en) * | 1979-04-13 | 1981-03-07 | Vizgazdalkodasi Tudomanyos Kut | Method and device for conditioning waste water sludge by ultrasonic wave |
| JPH04331000A (en) * | 1991-05-01 | 1992-11-18 | Kubota Corp | Dehydrating method of sludge |
| CN101344469A (en) * | 2008-05-14 | 2009-01-14 | 中国科学技术大学 | A granular sludge strength tester and its test method |
| CN103196781A (en) * | 2013-04-15 | 2013-07-10 | 杭州师范大学 | Measuring method and device for mechanical strength of granular sludge |
| CN103245623A (en) * | 2013-05-11 | 2013-08-14 | 北京工业大学 | Method for measuring relative hydrophobicity of granule sludge cells |
-
2014
- 2014-01-28 CN CN201410042419.3A patent/CN103776780B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5624099A (en) * | 1979-04-13 | 1981-03-07 | Vizgazdalkodasi Tudomanyos Kut | Method and device for conditioning waste water sludge by ultrasonic wave |
| JPH04331000A (en) * | 1991-05-01 | 1992-11-18 | Kubota Corp | Dehydrating method of sludge |
| CN101344469A (en) * | 2008-05-14 | 2009-01-14 | 中国科学技术大学 | A granular sludge strength tester and its test method |
| CN103196781A (en) * | 2013-04-15 | 2013-07-10 | 杭州师范大学 | Measuring method and device for mechanical strength of granular sludge |
| CN103245623A (en) * | 2013-05-11 | 2013-08-14 | 北京工业大学 | Method for measuring relative hydrophobicity of granule sludge cells |
Non-Patent Citations (4)
| Title |
|---|
| A.NOR-ANUAR ET AL.: "Strength characteristics of aerobic granular sludge", 《WATER SCIENCE & TECHNOLOGY》, vol. 65, no. 2, 31 December 2012 (2012-12-31), pages 309 - 316 * |
| 刘永红 等: "抗压实验法厌氧颗粒污泥机械强度的测定及影响因素分析", 《环境工程学报》, vol. 6, no. 11, 30 November 2012 (2012-11-30), pages 4197 - 4202 * |
| 李志华 等: "丝状菌颗粒污泥结构的形成及机理研究", 《中国环境科学学会学术年会论文集（2013）》, 1 August 2013 (2013-08-01) * |
| 栾先玉: "超声破碎剩余污泥的理论与实验研究", 《中国优秀硕士学位论文全文数据库 工程科技I辑》, no. 03, 15 March 2013 (2013-03-15) * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116067846A (en) * | 2022-12-15 | 2023-05-05 | 广东工业大学 | A method for testing the mechanical strength of aerobic granular sludge |
| CN116067846B (en) * | 2022-12-15 | 2023-08-22 | 广东工业大学 | A method for testing the mechanical strength of aerobic granular sludge |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103776780B (en) | 2017-04-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Nazari et al. | Aqueous phase adsorption of cephalexin by walnut shell-based activated carbon: A fixed-bed column study | |
| Wang et al. | Characterization of floc size, strength and structure in various aluminum coagulants treatment | |
| Zhu et al. | Chemiluminescence determination of ascorbic acid using graphene oxide@ copper-based metal–organic frameworks as a catalyst | |
| Li et al. | Label-free 2D colloidal photonic crystal hydrogel biosensor for urea and urease inhibitor | |
| Chen et al. | A graphene oxide surface–molecularly imprinted polymer as a dispersive solid-phase extraction adsorbent for the determination of cefadroxil in water samples | |
| CN103884669B (en) | Detect preparation method and the application thereof of mercury ion nanometer silver probe | |
| CN102095715A (en) | Surface enhanced Raman spectrum detection method for antioxidant BHT (Butylated Hydroxytoluene) | |
| Erdogan et al. | Characterization of the adsorption of disperse yellow 211 on activated carbon from cherry stones following microwave-assisted phosphoric acid treatment | |
| Yang et al. | A high-performance nonenzymatic piezoelectric sensor based on molecularly imprinted transparent TiO2 film for detection of urea | |
| CN105001862A (en) | Preparation of carbon quantum dots by use of aloe as carbon source and detection method of lemon yellow | |
| CN102353712B (en) | Preparation method of modified electrode for detecting uric acid and detection method | |
| Omar et al. | A sensing approach for manganese ion detection by carbon dots nanocomposite thin film-based surface plasmon resonance sensor | |
| CN103776780B (en) | Method for detecting strength of granular sludge | |
| CN103257133B (en) | Detect the preparation method and its usage of mercury ion probe | |
| CN103196781B (en) | Measuring method and device for mechanical strength of granular sludge | |
| Han et al. | Preparation of carbon quantum dot fluorescent probe from waste fruit peel and its use for the detection of dopamine | |
| Bertizzolo et al. | Monitoring settling of anaerobic digestates using low-field MRI profiling and NMR relaxometry measurements | |
| CN105891148A (en) | Method for measuring VFA concentration in anaerobic wastewater treatment process by means of near infrared spectrum | |
| CN104977267A (en) | Method for analyzing flocculation-ultrafiltration membrane pollution state and using amount of flocculating agent | |
| ATE411513T1 (en) | METHOD AND DEVICE FOR ABSORPTION SPECTROSCOPY | |
| CN103439309A (en) | Preparation method for high-sensitivity CO gas spectrum sensor | |
| CN103353410B (en) | A kind of pre-treating method measured for nitrogen P elements in alga cells in water | |
| CN103398981B (en) | A kind of waste oil detection method | |
| CN103439381A (en) | Method for detecting trace hydroquinone by using graphene/carbon nano tube modified electrode | |
| Xu et al. | Effect of different acid-modified coking coals on quinoline adsorption |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |