CN116063161A - 一种具有丝柏木香味的倍半萜化合物及其制备方法和应用 - Google Patents
一种具有丝柏木香味的倍半萜化合物及其制备方法和应用 Download PDFInfo
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- CN116063161A CN116063161A CN202211713809.XA CN202211713809A CN116063161A CN 116063161 A CN116063161 A CN 116063161A CN 202211713809 A CN202211713809 A CN 202211713809A CN 116063161 A CN116063161 A CN 116063161A
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Abstract
本发明属于天然香料化学技术领域，特别涉及一种具有丝柏木香味倍半萜化合物及其制备方法、包含其熏香液及用途。所述倍半萜类化合物的结构式如下：
本发明的倍半萜类化合物是从云南地方晾晒烟中分离得到的，具有显著的抗菌活性；还具有淡雅的丝柏木香味，略带药草香味，香韵特征非常独特。此外，发明还公开了上述倍半萜类化合物的制备方法、以及其作为熏香液的用途；化合物用作熏香液可显著抑制空气中的有害菌群，具有净化空气的功效。
Description
技术领域
本发明属于植物化学技术领域，具体涉及一种具有丝柏木香味的倍半萜化合物及其制备方法与应用。
背景技术
植物用于治疗疾病的历史跟人类历史同样久远，或许还更长远。我国自古就有薰香，古人懂得熏香具有美容、祛痛、消除疲劳、排解忧郁之功效。中华薰香文化浩如烟海，珍若珠肌，熏香能集天地之灵气，可怡情养性，可调和情态。利用纯天然药物的芳香气味及治愈能力，经由人的嗅觉器官或皮肤的吸收，通过神经和循环系统产生作用，使人的身体、心理、精神三者达到平衡和统一。香气，由于易渗透、高流动性和高吸入性的特点，可以通过人的皮肤或口鼻吸入，从而达到被人体吸收的效果。现代环境的污染对人类健康构成严重的危害，因此，综合利用天然植物成分，研究开发具有抗菌、净化空气作用的新型熏香品具有重要意义。
发明内容
本发明的第一目的在于提供一种具有丝柏木香味的倍半萜化合物；第二目的在于提供所述的具有丝柏木香味的倍半萜化合物的制备方法；第三目的在于提供所述的具有丝柏木香味的倍半萜化合物的应用。
本发明的第一目的是这样实现的，是以云南地方晾晒烟茎皮为原料，经前处理、浸膏提取、分子蒸馏、硅胶柱层析和高效液相色谱分离制备得到，命名为：5-甲氧基-3,6-二甲基-1-(2-甲亚丙基)-1
H-茚，英文名为：5-Methoxy-3,6-dimethyl-1-(2-methylpropylidene)-1
H-indene，其具有下述结构：
本发明的第二目的是这样实现的，是以云南地方晾晒烟茎皮为原料，经前处理、浸膏提取、分子蒸馏、硅胶柱层析和高效液相色谱分离制备得到，具体包括以下步骤：
A、前处理：将原料晾晒烟茎皮粉碎或切段得到物料a；
B、浸膏提取：将物料a中加入物料a质量2~6的有机提取溶剂，于常温下回流提取2~5次，每次提取时间为30~60min，合并提取液并过滤，减压浓缩到原体积的1/3~1/4至有沉淀物析出，静置20~60min，滤除沉淀物得到样品提取液，提取液减压浓缩得到浸膏b；
C、分子蒸馏：将浸膏b采用分子蒸馏初步分离，收集170~185℃的馏分为物料c；
D、硅胶柱层析：将物料c中加入物料c重量6~10倍量的160~200目硅胶装柱，以体积配比为20:1~1:1的氯仿-丙酮溶液进行梯度洗脱，经TLC监测，合并相同的部分；
每次要洗脱到没有组分流出，即蒸干溶剂后蒸馏瓶中没有成分残留，然后换下一个梯度；
E、高效液相色谱分离：将以8：2配比的氯仿-丙酮溶液进行洗脱得到的洗脱液经高压液相色谱分离纯化得到目标物具有丝柏木香味的倍半萜化合物。
所制备的具有丝柏木香味的倍半萜化合物结构是通过以下方法进行鉴定：
根据高分辨质谱的分子离子峰(
m/z251.1408，[M+Na]+)，可确定化合物的分子式为C16H20O，不饱和度为7。根据其核磁共振信号(图-1、图-2和表-1)，中有16个碳和20个氢，可归类为1个1,3,5,6-四取代的茚环(C-1~C-9，H-2，H-4和H-7)，一个2-甲亚丙基(=CH-CH(CH3)2，C-10~C-13，H-10，H-11，H6-12,13)，两个甲基(C-14，H3-14；C-15，H3-15)，以及一个甲氧基(
δ C56.1q，
δ H3.79s)。化合物中存在茚环，可进一步通过H-2和C-1、C-3、C-8、C-9，H-4和C-8、C-3、C-9，以及H-7和C-1、C-9的HMBC相关（图-2）得到证实。存在甲亚丙基(=CH-CH(CH3)2)结构也可通过H-10和C-11、C-12,13，H-11和C-10、C-12,13，H6-12,13和C-10的HMBC相关（图-2）和化合物中存在H3-12,13/H-11/H-10的1H-1HCOSY相关（图-2）得到确认。
化合物的亚甲基茚母体骨架确定后，其余的取代基团，甲亚丙基、甲氧基和两个甲基的位置也可通过分析其HMBC相关确定。甲亚丙基取代在茚环的C-1位，可通过H-10和C-8、C-1、C-2，以及H-11和C-1的HMBC相关得到证实。通过甲氧基信号(
δ H3.79)和C-5的HMBC相关，这可证实甲氧基取代在C-5位。此外，通过H3-14和C-2、C-3、C-9，以及H-2和C-14的HMBC相关可证实一个甲基取代在C-3位。通过H3-15和C-5、C-6、C-7，以及H-15和C-5的HMBC相关，可证实另一个甲基取代在C-6位。此外，C-1和C-10位的双键为E-构型，则可通过其耦合常数和文献对比确定。至此，化合物的结构得到准确确定，化合物命名为5-甲氧基-3,6-二甲基-1-(2-甲亚丙基)-1
H-茚，英文名为：5-Methoxy-3,6-dimethyl-1-(2-methylpropylidene)-1
H-indene。
化合物的红外、紫外和质谱数据：UV(甲醇),
λ max(log
ε)210(3.84)、262(3.29)、304(3.08)，IR(溴化钾压片)
ν max3070、2936、1618、1548、1462、1357、1158、1049、850、784cm-1；ESI-MS(正离子模式)
m/z251[M+Na]+；HR-ESI-MS(正离子模式)
m/z[M+Na]+251.1408(计算值251.1412，C16H20NaO)。
本发明的第三目的是这样实现的，所述的具有丝柏木香味的倍半萜化合物在制备抗菌产品中的应用。
所述的抗菌产品为抗菌熏香液。
所述的抗菌熏香液是由以下比例的原料制备而成：卷柏精油1.5～2%，松树精油1～1.5%，马郁兰净油0.8～1.5%，野橘精油0.8～1.5%，柠檬草精油0.6～1.2%，具有丝柏木香味的倍半萜类化合物0.5%～1%，三乙酸甘油酯10-20%，二乙二醇丁醚10～15%，丙二醇10～15%，其余部分为水。
上述各成分按比例混合均匀，并充分溶解，得到浅黄色透明的溶液，即为本发明的熏香液。该熏香液具有优雅的丝柏木香韵，略带甜香味。
在本发明熏香液配方组成中，各成分的具体效果如下：
二乙二醇丁醚为熏香液助溶剂，能增加配方中各组分的溶解性，使配方中的各组分能充分溶解，避免直接用水配制出现的微量沉淀和浑浊。
三乙酸甘油酯和丙二醇为定香剂，可使香料成分挥发均匀，防止快速蒸发，使香精香气更加持久。
卷柏精油为天然植物精油，具有优雅的松油味和很强的温暖感。具有宁神、助冥想的效果，有助于平静状态。
松树精油为天然植物精油，带有一种淡淡的清香味道，清新的香气使人仿佛置身森林之中，十分放松。具有提神，稳定情绪，抗菌，镇痛等功效，是有效的感冒和流感缓解剂。
马郁兰净油为天然植物净油，香味呈强烈的酚味、草味、木质味，非常类似百里香，具有舒缓焦虑与压力，强化心灵，温暖情绪。
野橘精油为天然植物精油，具有的甜蜜，柑橘的香气，可用于净化空气，促进和增强令人振奋的环境，或在家庭中散发出令人愉悦的气味。
柠檬草精油为天然植物精油，具有强烈的辛香味，略带甜香。对呼吸道的感染，如喉咙痛、喉炎与发烧具有显著的疗效；还能消除乳酸、促进循环，减轻运动后的肌肉疼痛，使肌肉柔软。
本发明熏香液的使用方法是将丝柏木香型抗菌熏香液放入熏香器中，打开熏香器电源，短时间内就散发出优雅的香味，熏香产生的气雾对于金黄色葡萄球菌、大肠杆菌、埃希菌、枯草杆菌、黑曲霉菌、变形杆菌的生长具有明显的抑制作用；具有显著的净化空气效果。此外，熏香产生的气雾还能缓解压力，放松不安的心情，缓解紧张性头痛。
烟草是一种重要的产香植物，烟草中含有丰富的香味化合物，在烟支燃烧过程中会释放出香味物质；因此尽管认为吸烟对健康有害，烟草还是以其丰富优雅的香味吸引了众多的消费者。虽然随着社会经济的发展，吸烟将受到越来越多的限制，但是烟草作为一个物种将长期存在，并继续会在国民经济中发挥着积极作用。因此，加强烟草非吸烟用途的研究非常重要。云南地方晾晒烟是烟草的云南特有变种，烟草从明万历末年进入云南，至清乾隆年间成为当地常见的农作物。云南自然条件好，温暖的河谷，冷凉的半山区、山区都适应烟草的生长。而且云南多样的气候、独特的地理环境，烟草在多年的栽培过程中形成了很多个具有地方特色的晾晒烟品种。这些晾晒烟在当地常盛产不衰，香气风格各具特色，且次生代谢产物研究报道较少。这为具有特色香气的新化合物发现提供了新样品来源。
国内外研究表明，绝大部分的香精香料(包括合成香料的源分子结构)均来自自然界，天然产物成为香精香料最重要的来源。在众多的香气成分分子结构中，倍半萜是一类非常重要的香源分子，许多倍半萜类分子结构具有花香、木香、琥珀样香等香调，香调非常丰富；由于倍半萜的沸点较高,常具有良好的定香作用，香气非常持久。为了发展和丰富烟草的非吸烟用途，为烟草的综合开发利用提供关键技术。本发明公开了一种晾晒烟中分离到的具有显著抗菌活性的新倍半萜类化合物，化合物具有淡雅的丝柏木香味，香韵特征非常独特。此外，还依托所述化合物开发了一种抗菌功效显著的熏香液。所述倍半萜具有优雅的丝柏木香香韵，还具有缓解压力，放松不安的心情，缓解紧张性头痛的功效。
本发明具有以下有益效果：
1、本发明以采收烟叶后的晾晒烟草茎皮为原料，首次提取出一种新的倍半萜类化合物，该化合物具有显著的抗菌活性，对金黄色葡萄球菌、大肠杆菌、埃希菌、枯草杆菌、黑曲霉菌、变形杆菌的抑制率超过91.8%。
2、申请人惊讶的发现，本发明的倍半萜类化合物具有优雅的丝柏木香味，略带药草香味，香韵特征非常独特。
3、此外，本发明首次制备出一种含有本发明的倍半萜类化合物的熏香液，制作简单，各成分按比例混合均匀即可得到。该熏香液具有优雅的丝柏木香香韵，能缓解压力，放松不安的心情，缓解紧张性头痛。还具有显著的抗菌活性和净化空气效果。
4、除本发明所述的倍半萜类化合物外，本发明熏香液的原料均可从市场买到，有成熟的生产和供应，熏香液制作成本低。熏香液中的关键成分倍半萜类化合物可以烟草茎皮为原料分离得到，原料来源非常广泛，成本低；生产该化合物可综合利用烟草种植、卷烟加工过程中的烟草废弃物。
5、对本发明化合物进行了毒理学评价，证实化合物安全无毒，可安全使用。
附图说明
图1本发明倍半萜类化合物的核磁共振碳谱（13C NMR）；
图2为本发明倍半萜类化合物的核磁共振氢谱（1H NMR）；
图3本发明倍半萜类化合物的关键HMBC和1H-1H COSY相关相关图。
具体实施方式
下面结合实施例和附图对本发明作进一步的说明，但不以任何方式对本发明加以限制，基于本发明教导所作的任何变换或替换，均属于本发明的保护范围。
本发明所述的具有丝柏木香味的倍半萜化合物，是以云南地方晾晒烟茎皮为原料，经前处理、浸膏提取、分子蒸馏、硅胶柱层析和高效液相色谱分离制备得到，命名为：5-甲氧基-3,6-二甲基-1-(2-甲亚丙基)-1
H-茚，英文名为：5-Methoxy-3,6-dimethyl-1-(2-methylpropylidene)-1
H-indene，其具有下述结构：
本发明所述的具有丝柏木香味的倍半萜化合物的制备方法，是以云南地方晾晒烟茎皮为原料，经前处理、浸膏提取、分子蒸馏、硅胶柱层析和高效液相色谱分离制备得到，具体包括以下步骤：
A、前处理：将原料晾晒烟茎皮粉碎或切段得到物料a；
B、浸膏提取：将物料a中加入物料a质量2~6的有机提取溶剂，于常温下回流提取2~5次，每次提取时间为30~60min，合并提取液并过滤，减压浓缩到原体积的1/3~1/4至有沉淀物析出，静置20~60min，滤除沉淀物得到样品提取液，提取液减压浓缩得到浸膏b；
C、分子蒸馏：将浸膏b采用分子蒸馏初步分离，收集170~185℃的馏分为物料c；
D、硅胶柱层析：将物料c中加入物料c重量6~10倍量的160~200目硅胶装柱，以体积配比为20:1~1:1的氯仿-丙酮溶液进行梯度洗脱，经TLC监测，合并相同的部分；
E、高效液相色谱分离：将以8：2配比的氯仿-丙酮溶液进行洗脱得到的洗脱液经高压液相色谱分离纯化得到目标物具有丝柏木香味的倍半萜化合物。
B步骤中所述的有机提取溶剂为质量浓度90%~100%甲醇水溶液、质量浓度90%~100%的乙醇水溶液或质量浓度70%~100%的丙酮水溶液。
D步骤上柱前还包括用物料c重量1.5~3倍的有机溶剂溶解后加入物料c重量0.8~1.2倍的80~100目硅胶进行拌样步骤。
所述的有机溶剂为纯甲醇或纯丙酮。
D步骤中所述的氯仿-丙酮溶液体积配比为20:1、9:1、8:2、7:3、6:4和1:1。
E步骤中高效液相色谱分离纯化是以体积浓度为60%的甲醇水溶液为流动相，流速15mL/min，以21.2×250mm，5μm的Zorbax PrepHT GF反相制备柱为固定相，紫外检测器检测波长为304nm，每次进样1.0~5.0mL，收集28.6min的色谱峰，多次累加后蒸干得到目标物具有丝柏木香味的倍半萜化合物。
本发明所述的具有丝柏木香味的倍半萜化合物的应用为所述的具有丝柏木香味的倍半萜化合物在制备抗菌产品中的应用。
所述的抗菌产品为抗菌熏香液。
所述的抗菌熏香液是由以下比例的原料制备而成：卷柏精油1.5～2%，松树精油1～1.5%，马郁兰净油0.8～1.5%，野橘精油0.8～1.5%，柠檬草精油0.6～1.2%，具有丝柏木香味的倍半萜类化合物0.5%～1%，三乙酸甘油酯10-20%，二乙二醇丁醚10～15%，丙二醇10～15%，其余部分为水。
本发明所用原料云南地方晾晒烟茎皮不受地区和品种限制，均可以实现本发明，下面以来源于云南不同产地的烟草茎皮原料对本发明做进一步说明：
实施例1
本实施例提供本发明所述的倍半萜类化合物的制备方法，所述制备方法包括：样品提取、硅胶柱层析和高效液相色谱分离步骤，将烟叶采收后的晾晒烟茎皮作为烟草原料，具体操作如下：
所用的晾晒烟品种为玉溪花铁杆烟，原料为烟叶采烤后的烟草茎皮，取晒干、粉碎后的样品，用体积浓度为95%的乙醇水溶液超声提取4次，每次30min。合并提取液，减压浓缩至体积的1/4；静置，滤除沉淀物，滤除沉淀后的溶液上MCI柱脱色，洗脱液减压浓缩得到浸膏；浸膏进一步进行分子蒸馏分离，收集170~195℃的馏分，得蒸馏产物。取分子蒸馏产物用丙酮溶解，然后加入100目硅胶拌样，用200目硅胶装柱，拌样后上柱；用体积比分别为20:1，9:1，8:2,7:3，6:4和1:1的氯仿-丙酮洗脱液进行梯度洗脱，收集梯度洗脱液、浓缩，经TLC监测，合并相同的部分，得到6个部分A-F，其中，对收集到的样品B(8:2)部分183g，再以60%的甲醇为流动相，流速20ml/min，21.2×250mm，5mm的Zorbax PrepHT GF反相制备柱为固定相，紫外检测器检测波长为304nm，每次进样5.0mL，收集28.6min的色谱峰，多次累加后蒸干，即得本发明所述的倍半萜类化合物。
根据高分辨质谱的分子离子峰(
m/z251.1408，[M+Na]+)，可确定化合物的分子式为C16H20O，不饱和度为7。根据其核磁共振信号(图-1、图-2和表-1)，中有16个碳和20个氢，可归类为1个1,3,5,6-四取代的茚环(C-1~C-9，H-2，H-4和H-7)，一个2-甲亚丙基(=CH-CH(CH3)2，C-10~C-13，H-10，H-11，H6-12,13)，两个甲基(C-14，H3-14；C-15，H3-15)，以及一个甲氧基(
δ C56.1q，
δ H3.79s)。化合物中存在茚环，可进一步通过H-2和C-1、C-3、C-8、C-9，H-4和C-8、C-3、C-9，以及H-7和C-1、C-9的HMBC相关（图-2）得到证实。存在甲亚丙基(=CH-CH(CH3)2)结构也可通过H-10和C-11、C-12,13，H-11和C-10、C-12,13，H6-12,13和C-10的HMBC相关（图-2）和化合物中存在H3-12,13/H-11/H-10的1H-1H COSY相关（图-2）得到确认。
化合物的亚甲基茚母体骨架确定后，其余的取代基团，甲亚丙基、甲氧基和两个甲基的位置也可通过分析其HMBC相关确定。甲亚丙基取代在茚环的C-1位，可通过H-10和C-8、C-1、C-2，以及H-11和C-1的HMBC相关得到证实。通过甲氧基信号(
δ H3.79)和C-5的HMBC相关，这可证实甲氧基取代在C-5位。此外，通过H3-14和C-2、C-3、C-9，以及H-2和C-14的HMBC相关可证实一个甲基取代在C-3位。通过H3-15和C-5、C-6、C-7，以及H-15和C-5的HMBC相关，可证实另一个甲基取代在C-6位。此外，C-1和C-10位的双键为E-构型，则可通过其耦合常数和文献对比确定。至此，化合物的结构得到准确确定，化合物命名为5-甲氧基-3,6-二甲基-1-(2-甲亚丙基)-1
H-茚，英文名为：5-Methoxy-3,6-dimethyl-1-(2-methylpropylidene)-1
H-indene。
化合物的红外、紫外和质谱数据：UV(甲醇),
λ max(log
ε)210(3.84)、262(3.29)、304(3.08)，IR(溴化钾压片)
ν max3070、2936、1618、1548、1462、1357、1158、1049、850、784cm-1；ESI-MS(正离子模式)
m/z251[M+Na]+；HR-ESI-MS(正离子模式)
m/z[M+Na]+251.1408(计算值251.1412，C16H20NaO)。
实施例2
对该化合物进行了抗菌活性筛选，体外抗菌实验用琼脂扩散法进行，首先将受试菌均匀地涂在普通琼脂培养基(牛肉膏、蛋白胨、氯化钠、血清、琼脂)的平板上，再将待测化合物(将实施例1得到的倍半萜类化合物用10mL DMSO溶解，加水稀释成50μg/mL的溶液)浸泡好的药片(用直径5mm圆形滤纸浸渍待测化合物溶液)放在带菌的培养基上，放入恒温箱内，于25℃孵育24-72h后观察抑菌圈大小。
结果表明：本发明化合物对金黄色葡萄球菌、大肠杆菌、埃希菌、枯草杆菌、黑曲霉菌、变形杆菌等具有很强的活性；抑制率超过91.8%。
实施例3
本实施例中的熏香液由以下原料制备而成（质量百分比）：卷柏精油1.5～2%，松树精油1～1.5%，马郁兰净油0.8～1.5%，野橘精油0.8～1.5%，柠檬草精油0.6～1.2%，权利要求1所述的倍半萜类化合物0.5%～1%，三乙酸甘油酯10-20%，二乙二醇丁醚10～15%，丙二醇10～15%，其余部分为水。以所述熏香液的总质量为基准。
上述各成分按比例混合均匀，并充分溶解，得到浅黄色透明的溶液，即为本发明的熏香液。该熏香液具有优雅的丝柏木香韵，略带甜香味。
将丝柏木香韵的抗菌熏香液放入熏香器中，打开熏香器电源，短时间内就散发出优雅的香味，熏香产生的气雾对于金黄色葡萄球菌、大肠杆菌、埃希菌、枯草杆菌、黑曲霉菌、变形杆菌的生长具有明显的抑制作用，具有显著的净化空气效果。此外，熏香产生的气雾还能缓解压力，放松不安的心情，缓解紧张性头痛。
实施例4
参照国家标准，《GB-T-16293-2016空气浮游菌测试方法》，在超净工作台中，将实施例3中熏香液放入熏香器中，打开电源开关，待产生的气雾稳定后，将涂布好的的平板开盖倒扣于烟雾上方距离熏香器50cm处，分别停留10min、30min、45min结束后于37℃恒温培养16h，每组重复3次，观察记录不同时间段的菌斑数和菌斑直径。从实验结果表明，在熏香30min左右，基本达到最大抑制抑菌作用。和对照相比，熏香液处理后的细菌总数、大肠菌群、金黄色葡萄球菌、绿脓杆菌、溶血性链球菌、真菌总数显著减少；对金黄色葡萄球菌、大肠杆菌、埃希菌、枯草杆菌、黑曲霉菌、变形杆菌的抑菌率全部达到45.6%以上，能够降低或消除空气中细菌滋生和繁殖的可能性。
实施例5
对实施例1中得到的新化合物进行了毒理学评价，通过小鼠骨髓微核实验、Ames实验和TK基因突变实验，用食用香精香料作为对照，三种毒理学测试结果均和对照无统计学上的差异；证明本发明化合物对动物无毒，使用安全。
上所述，仅为本发明的具体实施方式，但本发明的保护范围并不局限于此，任何熟悉本技术领域的技术人员在本发明揭露的技术范围内，可轻易想到变化或替换，都应涵盖在本发明的保护范围之内。因此，本发明的保护范围应以权利要求的保护范围为准。
Claims (10)
1.一种具有丝柏木香味的倍半萜化合物，其特征在于，是以云南地方晾晒烟茎皮为原料，经前处理、浸膏提取、分子蒸馏、硅胶柱层析和高效液相色谱分离制备得到，命名为：5-甲氧基-3,6-二甲基-1-(2-甲亚丙基)-1H-茚，英文名为：5-Methoxy-3,6-dimethyl-1-(2-methylpropylidene)-1H-indene，其具有下述结构：
。
2.一种权利要求1所述的具有丝柏木香味的倍半萜化合物的制备方法，其特征在于，是以云南地方晾晒烟茎皮为原料，经前处理、浸膏提取、分子蒸馏、硅胶柱层析和高效液相色谱分离制备得到，具体包括以下步骤：
A、前处理：将原料晾晒烟茎皮粉碎或切段得到物料a；
B、浸膏提取：将物料a中加入物料a质量2~6的有机提取溶剂，于常温下回流提取2~5次，每次提取时间为30~60min，合并提取液并过滤，减压浓缩到原体积的1/3~1/4至有沉淀物析出，静置20~60min，滤除沉淀物得到样品提取液，提取液减压浓缩得到浸膏b；
C、分子蒸馏：将浸膏b采用分子蒸馏初步分离，收集170~185℃的馏分为物料c；
D、硅胶柱层析：将物料c中加入物料c重量6~10倍量的160~200目硅胶装柱，以体积配比为20:1~1:1的氯仿-丙酮溶液进行梯度洗脱，经TLC监测，合并相同的部分；
E、高效液相色谱分离：将以8：2配比的氯仿-丙酮溶液进行洗脱得到的洗脱液经高压液相色谱分离纯化得到目标物具有丝柏木香味的倍半萜化合物。
3.根据权利要求2所述的制备方法，其特征在于，B步骤中所述的有机提取溶剂为质量浓度90%~100%甲醇水溶液、质量浓度90%~100%的乙醇水溶液或质量浓度70%~100%的丙酮水溶液。
4.根据权利要求2所述的制备方法，其特征在于，D步骤上柱前还包括用物料c重量1.5~3倍的有机溶剂溶解后加入物料c重量0.8~1.2倍的80~100目硅胶进行拌样步骤。
5.根据权利要求4所述的制备方法，其特征在于，所述的有机溶剂为纯甲醇或纯丙酮。
6.根据权利要求2所述的制备方法，其特征在于，D步骤中所述的氯仿-丙酮溶液体积配比为20:1、9:1、8:2、7:3、6:4和1:1。
7.根据权利要求2所述的制备方法，其特征在于，E步骤中高效液相色谱分离纯化是以体积浓度为60%的甲醇水溶液为流动相，流速15mL/min，以21.2×250mm，5μm的ZorbaxPrepHT GF反相制备柱为固定相，紫外检测器检测波长为304nm，每次进样1.0~5.0mL，收集28.6min的色谱峰，多次累加后蒸干得到目标物具有丝柏木香味的倍半萜化合物。
8.一种权利要求1所述的具有丝柏木香味的倍半萜化合物的应用，其特征在于，所述的具有丝柏木香味的倍半萜化合物在制备抗菌产品中的应用。
9.根据权利要求8所述的应用，其特征在于，所述的抗菌产品为抗菌熏香液。
10.根据权利要求9所述的应用，其特征在于，所述的抗菌熏香液是由以下比例的原料制备而成：卷柏精油1.5～2%，松树精油1～1.5%，马郁兰净油0.8～1.5%，野橘精油0.8～1.5%，柠檬草精油0.6～1.2%，具有丝柏木香味的倍半萜类化合物0.5%～1%，三乙酸甘油酯10-20%，二乙二醇丁醚10～15%，丙二醇10～15%，其余部分为水。
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