CN116042757A - Preparation method for extracting organic matter liquid by fusion of sea fresh water pearl layer powder - Google Patents

Preparation method for extracting organic matter liquid by fusion of sea fresh water pearl layer powder Download PDF

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CN116042757A
CN116042757A CN202211566913.0A CN202211566913A CN116042757A CN 116042757 A CN116042757 A CN 116042757A CN 202211566913 A CN202211566913 A CN 202211566913A CN 116042757 A CN116042757 A CN 116042757A
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pearl
powder
layer powder
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林展新
林海生
王雯
章超桦
谢郁
秦小明
曹文红
曾堉斯
谢绍河
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Guangdong Shaohe Pearl Co ltd
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Abstract

The invention provides a method for extracting organic matter liquid by fusion of sea fresh water pearl powder, which comprises the following steps: s1, mixing seawater pearl layer powder and fresh water pearl layer powder according to a certain proportion, and adopting multiple means such as high-temperature high-pressure treatment, ultrasonic waves, microwaves and the like to jointly modify; s2, adding protease according to a proper proportion for compound enzymolysis, and centrifuging after enzyme deactivation to obtain supernatant; s3, decoloring and concentrating to obtain the seawater and freshwater pearl powder fused organic matter liquid. The preparation method of the invention utilizes the complementary characteristics of sea water and fresh water pearl powder protein, greatly improves the efficiency of converting insoluble keratin into soluble polypeptide, has the advantages of operability, low energy consumption, high efficiency, green environmental protection and the like, and thereby improves the application value of the pearl powder in the fields of biological medicine and food.

Description

Preparation method for extracting organic matter liquid by fusion of sea fresh water pearl layer powder
Technical Field
The invention belongs to the technical field of biology, and particularly relates to a method for extracting organic matter liquid by fusion of sea fresh water pearl layer powder.
Background
The pearl is produced by stimulating and secreting calcium carbonate-containing mineral beads from bivalve animals such as Margaritifera pearl shell of Pearl family, hyriopsis cumingii or Cristaria plicata of mussel family, and has two kinds of fresh water pearl and seawater pearl.
The pearl has high market value as precious organic jewelry on one hand, and receives attention as traditional Chinese medicine on the other hand. The pearl powder recorded in the compendium of materia medica has various effects, and modern medicine further demonstrates the functional activities of the pearl powder, such as improving eyesight, stabilizing, detoxifying, resisting aging, whitening, assisting in reducing blood pressure and the like.
The pearl shell is a main byproduct of pearl shell processing, and most of the pearl shell is discarded as waste or simply processed into building materials at present from the aspect of processing utilization degree. As pearl oyster can be an important biomineralization material, the main components of the pearl oyster are calcium carbonate and organic matters, and the pearl oyster has great application value in the fields of environmental pollution control, high polymer materials, coatings, biological medicines and the like. Research shows that the main component of the pearl powder is very similar to the pearl powder, has various biological activities and has higher potential development and utilization values.
The pearl shell is divided into three layers, the horny layer and the prismatic layer are removed, and the fine powder processed by the rest pearl layer is called pearl layer powder. The nacreous layer powder is white powder ground from nacreous layer of nacreous layer shell, has slight fishy smell, and comprises inorganic component, organic component and water, and the contents of different kinds of nacreous layer powder are slightly different, generally the nacreous layer powder contains inorganic component 90% -96%, organic component 2.0% -7.0%, and water 0.2% -2.0%. The inorganic component is mainly calcium carbonate, and contains oxides of various microelements (Cu, mg, sr, se, cr, mn, fe, al, etc.). The main body of the organic component of the nacreous layer is various proteins and polysaccharide chitin secreted by the mantle cells of pearl shells and mussels, the proteins are mainly shell keratin, and the organic matters and the inorganic matters are firmly combined, so that the nacreous layer has the characteristics of water insolubility and the like, and is difficult to directly use. The content of organic matters in the pearl layer is obviously different from the processing mode and variety, the protein content of the fresh water pearl layer is generally higher than that of the seawater pearl layer, and the amino acid composition and the characteristics of the protein in the fresh water pearl layer and the seawater pearl layer are also greatly different (shown in table 1).
In recent years, as research on pearl efficacy factors continues to be in progress, organic matters such as pearl proteins and the like are one of main active ingredients, and attention of researchers at home and abroad is paid. The acid method is used for dissolving the pearl powder to prepare the soluble pearl powder and degrading insoluble protein, but the strong acid has the defects of high protein degradation degree, main free amino acid as a product, environmental pollution, peculiar smell of hydrolysis products and the like; although weak acid combined enzymolysis can effectively degrade inorganic matters such as insoluble calcium carbonate and the like and degrade insoluble proteins to a certain extent, the product is a large amount of complex components such as organic acid radical ions, calcium ions, free amino acids, polypeptides and the like, and is difficult to effectively separate and apply, and particularly calcium carbonate or calcium ions have great influence on aspects such as compatibility and use, stability maintenance, efficacy activity and the like of the product in the field of cosmetics.
The pearl protein is often combined with inorganic matters and crosslinked with each other to form a stronger network structure, and the shell keratin structure is more complex, and the functions of hydrogen bond, hydrophobic bond, disulfide bond and the like are realized, so that the peptide chain is tightly curled, the chemical property of the pearl protein is particularly stable, the mechanical strength is higher, and the pearl protein is not easy to dissolve and digest. If the small molecular peptide is extracted by adopting a direct enzymolysis method, the yield is low.
Although proper pretreatment (including physical, chemical and enzymatic) is disclosed in the prior art, the composition and structure of the protein can be denatured or changed to improve the hydrolysis degree and shorten the enzymolysis time, the application effect in the pearl powder is not ideal, the content of the extracted amino acid and small molecular peptide is low, and the resources cannot be fully utilized.
Disclosure of Invention
The invention provides a method for extracting organic matter liquid by fusion of sea fresh water pearl layer powder. By utilizing the difference of protein of the seawater pearl layer powder and the fresh water pearl layer powder, the pearl powder is modified by adopting a combined physical modification method, and then insoluble protein is effectively degraded by adopting a high-efficiency composite enzymolysis technology to prepare an organic matter liquid, so that the organic fusion of the two pearl powders and the advantage complementation of amino acid and hydrolytic peptide are realized.
The raw materials adopted by the invention comprise seawater pearl layer powder, fresh water pearl layer powder, pretreatment liquid, protease and the like, and have no toxic or side effect. After the nacreous layer powder is subjected to combined physical modification, a mild controllable biological enzymolysis technology is adopted, no chemical agent is added, and an enzymolysis product has no toxic or side effect, so that a safe and green environment-friendly process is realized, and the application range of the prepared organic matter liquid is wider.
The technical scheme of the invention is as follows:
the method for extracting the organic matter liquid by fusion of the seawater and freshwater pearl powder is characterized by comprising the following steps of:
s1, mixing seawater pearl layer powder and fresh water pearl layer powder according to a certain proportion, adding pretreatment liquid, and performing physical modification, wherein the physical modification comprises any two of the following combinations: high-temperature high-pressure treatment, ultrasonic wave and microwave;
s2, adding protease according to a proper proportion for compound enzymolysis, and centrifuging after enzyme deactivation to obtain supernatant;
s3, decoloring and concentrating to obtain the seawater and freshwater pearl powder fused organic matter liquid.
Further, in the step S1, the seawater pearl shell and the fresh water pearl shell are respectively cleaned, dried and crushed, and are sieved by a 500-200-mesh sieve to respectively obtain seawater pearl layer powder and fresh water pearl layer powder; the seawater pearl layer powder and the fresh water pearl layer powder are mixed and modified according to the mass ratio of 2:3-8.
According to the invention, the adopted mixing proportion of the seawater pearl layer powder and the fresh water pearl layer powder can realize the advantage complementation of the amino acid composition difference of the fresh water pearl layer and the seawater pearl layer protein, and meanwhile, the mutual synergistic effect can be generated in the reaction process, so that the conversion and release of small molecular active substances are facilitated.
Further, in step S1, the pretreatment liquid includes the following components in parts by weight: 33-55 parts of erythritol, 12-19 parts of sodium chloride, 23-38 parts of ascorbic acid and 13-29 parts of citric acid.
The pretreatment liquid adopted by the invention comprises the following components:
erythritol, which is a four-carbon sugar alcohol, is a bulk sweetener, can be prepared by fermenting glucose, is white crystalline powder, has refreshing sweetness, is not easy to absorb moisture, is stable at high temperature, and is stable in a wide pH range.
Ascorbic acid is white crystal or crystalline powder, odorless, sour, and gradually changed to yellowish after long-term storage. The aqueous solution shows an acidic reaction. Is soluble in water, slightly soluble in ethanol, and insoluble in chloroform or diethyl ether; having a dienol structure, the acidity corresponds to that of a monoacid; meanwhile, the catalyst is an effective reducing agent and an antioxidant.
Citric acid is colorless semitransparent crystal or white particles or white crystalline powder at room temperature, has no odor and extremely acidic taste, has astringency, weak corrosiveness and strong deliquescence, and is accompanied with the generation of crystalline hydrate; citric acid is a strong organic acid with 3H' s + Can ionize; the heating can be decomposed into various products, and the products react with acid, alkali, glycerin and the like.
The pretreatment liquid can be compounded in a synergistic way under the condition of physical modification, and reacts with citric acid in calcium carbonate through ascorbic acid to form soluble calcium ions, and under the action of sodium chloride, precipitation is prevented, and under the action of erythritol, a stable reaction system is formed to prevent reversible reaction. The method comprises the following steps: under the condition of physical modification, the pretreatment liquid acts on the space network structure inside the pearl layer powder to destroy the bonding force of the pearl layer powder, prevent the occurrence of cross linking, and has certain destructive effects on hydrogen bonds, hydrophobic bonds, disulfide bonds and the like inside the shell keratin, so that the keratin of the pearl layer powder can be more fully degraded into small molecular peptides with biological activity in the subsequent enzymolysis, and the high-value utilization of the pearl layer powder is realized.
Further, in the step S1, the addition amount of the pretreatment liquid is 15-33% of the mass of the pearl layer powder.
Further, in the physical modification, the conditions of high temperature and high pressure are as follows: 115-121 ℃ for 20-30min; ultrasonic treatment: 50-60 ℃,40-50min and 100-200W; the conditions of the microwave treatment are as follows: 350-700W,4-8min.
The physical modification method of the invention mainly uses modes such as heat, microwave, sound wave, high pressure and the like to change the structure and the property of the protein, and has the advantages of low cost, no toxic or side effect, small damage to the protein and the like.
Under the condition of high temperature and high pressure, the molecular structure is changed, and particularly the internal rotation angle has an important influence on chemical reaction. The high temperature condition is favorable for the cracking of reactants and the formation of free radicals, and improves the chemical reaction rate. In the gas phase under high pressure conditions, when bubbles are broken to generate high pressure, strong shock waves are accompanied, so that molecules are strongly mutually collided and aggregated.
The effect of the ultrasonic wave is that tiny bubbles in the liquid are activated under the action of an ultrasonic field, and the tiny bubbles are expressed as a series of dynamic processes such as formation, oscillation, growth, shrinkage, collapse and the like of bubble nuclei, and physical and chemical effects caused by the dynamic processes. The bubbles collapse suddenly within a few microseconds, similar to a small explosion process, creating a very brief high energy environment, thereby creating localized high temperature, high pressure.
Under the action of microwave magnetic field, partial polar molecules rotate along the action direction of external electric field to tend to be regularly arranged, and the direction is changed at high frequency along with the change of the positive and negative of the energy field. During the high-speed movement, the molecules can collide and rub with each other vigorously to generate heat.
In the invention, by combining physical modification and pretreatment liquid treatment, special reaction conditions are provided for chemical reactions which are difficult or impossible to realize under general conditions, so that the internal structure of the nacreous layer powder is further changed, and the decomposition of a macromolecular structure and the release of a micromolecular structure are facilitated.
In step S2, the enzyme selected for enzymolysis is any two of bromelain, keratinase, neutral protease and papain, and the addition amount of bromelain is as follows: 0.10-0.30g/100g, the added amount of keratinase is as follows: 0.5-2.0g/100g, and the addition amount of neutral protease is as follows: 0.2-0.6g/100g, papain addition amount is as follows: 0.05-0.20g/100g, and enzymolysis parameters are as follows: the pH value is 6.0-7.0, the enzymolysis temperature is 45-60 ℃, after the enzymolysis time is 4-7h, the enzyme is inactivated for 20 minutes at 100 ℃, and the supernatant is obtained by centrifugation.
Through the composite enzymolysis reaction, the enzymolysis efficiency and the yield of the micromolecular active substances can be improved, and meanwhile, the extracted micromolecular active peptide has high activity and pertinence, and the specific biological activity is endowed.
Further, in step S3, after the activated carbon and diatomite are used for combined decolorization by adopting the prior art method, the polypeptide content is concentrated in vacuum: 10.0-30.0mg/ml (measured by BCA method), the seawater-freshwater pearl powder fusion organic matter liquid is light yellow transparent clear liquid.
The invention also provides application of the organic matter liquid in the production of pearl wine, nutritional functional food and beauty and skin care products.
The invention provides a method for extracting organic matter liquid by fusion of sea fresh water pearl layer powder, which is characterized in that macromolecules are converted into small molecules before enzymolysis by combining pretreatment liquid intervention and physical modification treatment, so that the content of the active substances of the small molecules in the subsequent compound enzymolysis can be remarkably improved, and the technical problems of poor water solubility, difficult utilization and the like of pearl layer proteins in the prior art can be solved.
The applicant finds out through a large number of creative experiments that the combination of a reasonable pretreatment mode and protease enzymolysis plays a key role in degrading insoluble pearl protein, and can solve the technical problems of poor water solubility, difficult utilization and the like of pearl layer proteins in the prior art.
The organic matter liquid prepared by the invention utilizes the difference of the proteins of the seawater pearl layer powder and the freshwater pearl layer powder to realize the organic fusion of the two pearl powders and the advantage complementation of amino acid and hydrolytic peptide, and can improve the conversion rate of micromolecular substances in the pretreatment process.
The preparation method of the invention not only effectively improves the added value of the pearl oyster shell waste, but also expands the functional application range of the pearl oyster shell waste, can be applied to the fields of producing pearl wine, nutritional functional foods, beauty and skin care products and the like, and has higher economic value.
Drawings
FIG. 1 is a standard graph for determining hydrolysis rate;
FIG. 2 is an ultraviolet absorption spectrum of the organic liquid prepared in example 2 of the present invention.
Detailed Description
The technical solutions of the present invention will be clearly and completely described in conjunction with the embodiments of the present invention, and it is apparent that the described embodiments are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Example 1
The horny layer and prismatic layer of sea water pearl shell (Pinctada martensii) and fresh water pearl shell (hyriopsis cumingii) are removed respectively, and the sea water pearl shell (Pinctada martensii) and fresh water pearl shell (hyriopsis cumingii) are cleaned, dried and crushed into powder with the size of more than 200 meshes, and the protein amino acid composition is detected and analyzed by the prior art, and the result is shown in table 1.
TABLE 1 protein amino acid composition of seawater nacreous layer powder and fresh water nacreous layer powder
Figure DEST_PATH_IMAGE001
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As can be seen from Table 1, the relative amounts of essential amino acids, semi-essential amino acids, and optional amino acids in the seawater pearl layer powder and the freshwater pearl layer powder by the direct detection method are shown in Table 2.
Figure DEST_PATH_IMAGE002
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Example 2
The method for extracting the organic matter liquid by fusion of the seawater and freshwater pearl powder is characterized by comprising the following steps of:
s1, mixing seawater pearl layer powder and fresh water pearl layer powder according to a certain proportion, adding pretreatment liquid, and performing physical modification, wherein the physical modification comprises the steps of treating at high temperature and high pressure firstly and then performing ultrasonic treatment;
s2, adding at least two proteases according to a proper proportion for compound enzymolysis, and centrifuging after enzyme deactivation to obtain a supernatant;
s3, decoloring and concentrating to obtain the seawater and freshwater pearl powder fused organic matter liquid.
Further, in step S1, the seawater pearl shell and the fresh water pearl shell are respectively cleaned, dried and crushed, and are sieved by a 300-mesh sieve to respectively obtain seawater pearl layer powder and fresh water pearl layer powder; the seawater pearl layer powder and the fresh water pearl layer powder are mixed and modified according to the mass ratio of 1:2.
Further, in step S1, the pretreatment liquid includes the following components in parts by weight: erythritol 46, sodium chloride 16, ascorbic acid 29, and citric acid 21.
Further, the addition amount of the pretreatment liquid is 23% of the mass of the pearl layer powder.
Further, in the physical modification, the conditions of high temperature and high pressure are as follows: 121 ℃ for 20min; ultrasonic treatment: 50 ℃,40min,100W.
In step S2, the enzyme selected for enzymolysis is two compounds of bromelain and neutral protease, and the addition amount of bromelain is as follows: 0.10g/100g, the addition amount of neutral protease enzyme is as follows: 0.2g/100g, papaya enzymolysis parameters are: and the pH value is 6.5, the enzymolysis temperature is 50 ℃, after the enzymolysis time is 4 hours, the enzyme is inactivated for 20 minutes at 100 ℃, and the supernatant is obtained by centrifugation.
Adding activated carbon and diatomite (0.01% W/V) into the supernatant obtained in the step S3 according to the addition amount of 0.3% (W/V), preserving heat at 50 ℃ for 20min, centrifuging at 12000g for 20min, removing the decoloring agent, concentrating at 65 ℃ under vacuum at the concentration of polypeptide content of at most: 30mg/ml (measured by BCA method) to obtain seawater-freshwater pearl powder fusion organic matter liquid, which is characterized by light yellow transparent clear liquid; the organic liquid had a maximum absorption peak at ultraviolet 204nm (FIG. 1).
Example 3
The embodiment provides a method for extracting organic matter liquid by fusion of seawater and freshwater pearl powder, which is the same as that in embodiment 2, wherein in step S1, the pretreatment liquid comprises the following components in parts by weight: erythritol 35, sodium chloride 14, ascorbic acid 23-38 and citric acid 15.
Further, the addition amount of the pretreatment liquid is 17% of the mass of the pearl layer powder.
Example 4
The embodiment provides a method for extracting organic matter liquid by fusion of seawater and freshwater pearl powder, which is the same as that in embodiment 2, wherein in step S1, the pretreatment liquid comprises the following components in parts by weight: erythritol 52, sodium chloride 17, ascorbic acid 35, and citric acid 26.
Further, the addition amount of the pretreatment liquid is 30% of the mass of the pearl layer powder.
Example 5
This example provides a method for extracting organic matter liquid by fusion of sea fresh water pearl layer powder as in example 2, except that,
step S1, respectively cleaning, drying and crushing seawater pearl shells and freshwater pearl shells, and sieving with a 400-mesh sieve to respectively obtain seawater pearl layer powder and freshwater pearl layer powder; the seawater pearl layer powder and the fresh water pearl layer powder are mixed and modified according to the mass ratio of 2:3.
Example 6
This example provides a method for extracting organic matter liquid by fusion of sea fresh water pearl layer powder as in example 2, except that,
step S1, respectively cleaning, drying and crushing seawater pearl shells and freshwater pearl shells, and sieving with a 200-mesh sieve to respectively obtain seawater pearl layer powder and freshwater pearl layer powder; the seawater pearl layer powder and the fresh water pearl layer powder are mixed and modified according to the mass ratio of 2:7.
Example 7
This example provides a method for extracting organic matter liquid by fusion of sea fresh water pearl layer powder as in example 2, except that,
the physical modification adopts the following combination: firstly, carrying out microwave treatment and then carrying out ultrasonic treatment;
ultrasonic treatment: 52 ℃,48min,180W; the conditions of the microwave treatment are as follows: 450W,6min.
Example 8
This example provides a method for extracting organic matter liquid by fusion of sea fresh water pearl layer powder as in example 2, except that,
the physical modification adopts the following combination: firstly, treating at high temperature and high pressure, and then treating by microwaves;
in the physical modification, the conditions of high temperature and high pressure are as follows: 120 ℃ for 25min; the conditions of the microwave treatment are as follows: 600W,5min.
Example 9
This example provides a method for extracting organic matter liquid by fusion of sea fresh water pearl layer powder as in example 2, except that,
in step S2, the enzyme selected for enzymolysis is two compounds of bromelain and keratinase, and the addition amount of bromelain is as follows: 0.2g/100g, the added amount of the keratinase enzyme is as follows: 0.6g/100g, and the enzymolysis parameters are as follows: and the pH value is 6.0, the enzymolysis temperature is 52 ℃, the enzymolysis time is 4.5 hours, the enzyme is inactivated at 100 ℃ for 20 minutes, and the supernatant is obtained by centrifugation.
Example 10
This example provides a method for extracting organic matter liquid by fusion of sea fresh water pearl layer powder as in example 2, except that,
in step S2, the enzyme selected for enzymolysis is two compounds of bromelain and papain, and the addition amount of bromelain is as follows: 0.25g/100g, papain addition to: 0.06g/100g, the enzymolysis parameters are as follows: and the pH value is 7.0, the enzymolysis temperature is 55 ℃, after the enzymolysis time is 5 hours, the enzyme is inactivated for 20 minutes at 100 ℃, and the supernatant is obtained by centrifugation.
Example 11
This example provides an application of the organic matter liquid prepared in example 2 in the production of pearl wine, nutritional functional food and cosmetic and skin care products.
Comparative example 1
The present example provides a method for extracting an organic liquid from the same nacreous layer powder as in example 2, except that in step S1, seawater nacreous layer powder is used.
Comparative example 2
The present example provides a method for extracting an organic liquid from the same nacreous layer powder as in example 2, except that in step S1, a fresh water nacreous layer powder is used.
Comparative example 3
This example provides a method for extracting an organic liquid from the same nacreous layer powder as in example 2, except that no pretreatment liquid is added in step S1.
Comparative example 4
This example provides a method for extracting an organic matter solution from the same nacreous layer powder as in example 2, except that in step S1, erythritol is not contained in the pretreatment solution.
Comparative example 5
This example provides a method for extracting an organic matter solution from the same nacreous layer powder as in example 2, except that in step S1, the pretreatment solution does not contain sodium chloride.
Comparative example 6
The present example provides a method for extracting an organic matter solution from the same nacreous layer powder as in example 2, except that in step S1, the pretreatment solution does not contain ascorbic acid or citric acid.
Comparative example 7
This example provides a method for extracting an organic liquid from the same nacreous layer powder as in example 2, except that in step S1, no physical modification treatment is performed.
Test of experimental Effect
The protein amino acid compositions of the organic matter solutions prepared in example 2, comparative example 1 and comparative example 2 were respectively examined and analyzed by the test method of the prior art, and the relative contents of essential amino acids, semi-essential amino acids and non-essential amino acids were counted, and the results are shown in Table 3.
TABLE 3 Table 3
Figure DEST_PATH_IMAGE003
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The BCA kit is adopted to detect the protein/peptide content in the supernatant, the protein concentration standard curve is shown in figure 1, the protein concentration and the absorbance value are in linear relation, and the correlation degree is good. After measuring the protein concentration of the enzymatic supernatant, the Degree of Hydrolysis (DH) was used as an index for evaluating the enzymatic hydrolysis effect. DH (%) =concentration of protein peptide in supernatant (mg/mL), total volume of supernatant (mL)/weight of pearl powder (g), total protein content (%) 1000×100. The organic liquids prepared in examples 2 to 10 and comparative examples 1 to 7 were each tested for their degree of hydrolysis, and the results are shown in Table 4.
TABLE 4 Table 4
Figure DEST_PATH_IMAGE004
。
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. The present embodiments are, therefore, to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.
Furthermore, it should be understood that although the present disclosure describes embodiments, not every embodiment is provided with a separate embodiment, and that this description is provided for clarity only, and that the disclosure is not limited to the embodiments described in detail below, and that the embodiments described in the examples may be combined as appropriate to form other embodiments that will be apparent to those skilled in the art. It should be noted that technical features not described in detail in the present invention may be implemented by any prior art.

Claims (8)

1. The method for extracting the organic matter liquid by fusion of the seawater and freshwater pearl powder is characterized by comprising the following steps of:
s1, mixing seawater pearl layer powder and fresh water pearl layer powder according to a certain proportion, adding pretreatment liquid, and performing physical modification, wherein the physical modification comprises any two of the following combinations: high-temperature high-pressure treatment, ultrasonic wave and microwave;
s2, adding at least two proteases according to a proper proportion for compound enzymolysis, and centrifuging after enzyme deactivation to obtain a supernatant;
s3, decoloring and concentrating to obtainSea fresh water pearl powder fused organic matter liquid。
2. The preparation method according to claim 1, wherein in step S1, seawater pearl shell and freshwater pearl shell are respectively washed, dried and crushed, and pass through a 500-200 mesh sieve to obtain seawater pearl layer powder and freshwater pearl layer powder respectively; the seawater pearl layer powder and the fresh water pearl layer powder are mixed and modified according to the mass ratio of 2:3-8.
3. The preparation method according to claim 2, wherein in step S1, the pretreatment liquid comprises the following components in parts by weight: 33-55 parts of erythritol, 12-19 parts of sodium chloride, 23-38 parts of ascorbic acid and 13-29 parts of citric acid.
4. The method according to claim 3, wherein the pretreatment liquid is added in an amount of 15-33% by mass of the pearl powder in step S1.
5. The method according to any one of claims 2 to 4, wherein the conditions of high temperature and high pressure in the physical modification are: 115-121 ℃ for 20-30min; ultrasonic treatment: 50-60 ℃,40-50min and 100-200W; the conditions of the microwave treatment are as follows: 350-700W,4-8min.
6. The preparation method of claim 5, wherein in step S2, the enzyme selected for enzymolysis is any two of bromelain, keratinase, neutral protease and papain, and the addition amount of bromelain is as follows: 0.10-0.30g/100g, the added amount of keratinase is as follows: 0.5-2.0g/100g, and the addition amount of neutral protease is as follows: 0.2-0.6g/100g, papain addition amount is as follows: 0.05-0.20g/100g, and enzymolysis parameters are as follows: the pH value is 6.0-7.0, the enzymolysis temperature is 45-60 ℃, after the enzymolysis time is 4-7h, the enzyme is inactivated for 20 minutes at 100 ℃, and the supernatant is obtained by centrifugation.
7. The method according to claim 6, wherein in step S3, after the activated carbon and the diatomite are used for combined decolorization, the polypeptide is concentrated in vacuum to a content of polypeptide of: 10.0-30.0mg/ml to obtainThe seawater and freshwater pearl powder is fused with organic matter liquid.
8. Use of the organic matter liquid according to any one of claims 1 to 7 for the production of pearl wine, nutritional functional food, and cosmetic skin care products.
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