CN116042326A - Nucleic acid pollution scavenger - Google Patents
Nucleic acid pollution scavenger Download PDFInfo
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- CN116042326A CN116042326A CN202211308288.XA CN202211308288A CN116042326A CN 116042326 A CN116042326 A CN 116042326A CN 202211308288 A CN202211308288 A CN 202211308288A CN 116042326 A CN116042326 A CN 116042326A
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- nucleic acid
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- sodium
- scavenger
- potassium carbonate
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- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 69
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 69
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 69
- 239000002516 radical scavenger Substances 0.000 title claims abstract description 35
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims abstract description 45
- BWHMMNNQKKPAPP-UHFFFAOYSA-L potassium carbonate Chemical compound [K+].[K+].[O-]C([O-])=O BWHMMNNQKKPAPP-UHFFFAOYSA-L 0.000 claims abstract description 42
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 30
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims abstract description 28
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 28
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims abstract description 26
- 229910000027 potassium carbonate Inorganic materials 0.000 claims abstract description 21
- 235000019156 vitamin B Nutrition 0.000 claims abstract description 18
- 239000011720 vitamin B Substances 0.000 claims abstract description 18
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims abstract description 14
- 235000019341 magnesium sulphate Nutrition 0.000 claims abstract description 14
- 229920000136 polysorbate Polymers 0.000 claims abstract description 14
- 239000011780 sodium chloride Substances 0.000 claims abstract description 14
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims abstract description 14
- 229960001763 zinc sulfate Drugs 0.000 claims abstract description 14
- 229910000368 zinc sulfate Inorganic materials 0.000 claims abstract description 14
- 239000001103 potassium chloride Substances 0.000 claims abstract description 13
- 235000011164 potassium chloride Nutrition 0.000 claims abstract description 13
- 230000002000 scavenging effect Effects 0.000 claims abstract description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 12
- 239000000356 contaminant Substances 0.000 claims abstract description 10
- VPMBPDKLCXQCSR-UHFFFAOYSA-N sodium;1,3,5-trichloro-1,3,5-triazinane-2,4,6-trione Chemical compound [Na].ClN1C(=O)N(Cl)C(=O)N(Cl)C1=O VPMBPDKLCXQCSR-UHFFFAOYSA-N 0.000 claims abstract description 10
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 9
- 239000011734 sodium Substances 0.000 claims abstract description 9
- 235000011121 sodium hydroxide Nutrition 0.000 claims abstract description 7
- 239000008367 deionised water Substances 0.000 claims abstract description 6
- 229910021641 deionized water Inorganic materials 0.000 claims abstract description 6
- 238000002360 preparation method Methods 0.000 claims abstract description 4
- 238000011109 contamination Methods 0.000 claims description 8
- 229930003270 Vitamin B Natural products 0.000 claims description 6
- 239000007921 spray Substances 0.000 claims description 5
- 230000000694 effects Effects 0.000 claims description 4
- 238000000889 atomisation Methods 0.000 claims description 3
- 238000007654 immersion Methods 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 3
- 238000005507 spraying Methods 0.000 abstract description 4
- 239000004480 active ingredient Substances 0.000 abstract description 2
- 239000007864 aqueous solution Substances 0.000 abstract description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 abstract description 2
- 229910052760 oxygen Inorganic materials 0.000 abstract description 2
- 239000001301 oxygen Substances 0.000 abstract description 2
- 238000002791 soaking Methods 0.000 abstract description 2
- 230000001988 toxicity Effects 0.000 abstract description 2
- 231100000419 toxicity Toxicity 0.000 abstract description 2
- 230000015572 biosynthetic process Effects 0.000 abstract 1
- WQYVRQLZKVEZGA-UHFFFAOYSA-N hypochlorite Chemical compound Cl[O-] WQYVRQLZKVEZGA-UHFFFAOYSA-N 0.000 description 4
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 239000004744 fabric Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 229910052708 sodium Inorganic materials 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 1
- ISAOUZVKYLHALD-UHFFFAOYSA-N 1-chloro-1,3,5-triazinane-2,4,6-trione Chemical compound ClN1C(=O)NC(=O)NC1=O ISAOUZVKYLHALD-UHFFFAOYSA-N 0.000 description 1
- GHOKWGTUZJEAQD-UHFFFAOYSA-N Chick antidermatitis factor Natural products OCC(C)(C)C(O)C(=O)NCCC(O)=O GHOKWGTUZJEAQD-UHFFFAOYSA-N 0.000 description 1
- AUNGANRZJHBGPY-UHFFFAOYSA-N D-Lyxoflavin Natural products OCC(O)C(O)C(O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-UHFFFAOYSA-N 0.000 description 1
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 description 1
- AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 description 1
- XSTXAVWGXDQKEL-UHFFFAOYSA-N Trichloroethylene Chemical compound ClC=C(Cl)Cl XSTXAVWGXDQKEL-UHFFFAOYSA-N 0.000 description 1
- 229930003451 Vitamin B1 Natural products 0.000 description 1
- 229930003471 Vitamin B2 Natural products 0.000 description 1
- 229930003537 Vitamin B3 Natural products 0.000 description 1
- 229930003571 Vitamin B5 Natural products 0.000 description 1
- 229930003756 Vitamin B7 Natural products 0.000 description 1
- 229930003761 Vitamin B9 Natural products 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 239000000443 aerosol Substances 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- FAPWYRCQGJNNSJ-UBKPKTQASA-L calcium D-pantothenic acid Chemical compound [Ca+2].OCC(C)(C)[C@@H](O)C(=O)NCCC([O-])=O.OCC(C)(C)[C@@H](O)C(=O)NCCC([O-])=O FAPWYRCQGJNNSJ-UBKPKTQASA-L 0.000 description 1
- 229960002079 calcium pantothenate Drugs 0.000 description 1
- 230000007797 corrosion Effects 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 229960003512 nicotinic acid Drugs 0.000 description 1
- DFPAKSUCGFBDDF-UHFFFAOYSA-N nicotinic acid amide Natural products NC(=O)C1=CC=CN=C1 DFPAKSUCGFBDDF-UHFFFAOYSA-N 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- 150000002978 peroxides Chemical class 0.000 description 1
- 229960002816 potassium chloride Drugs 0.000 description 1
- 229960002477 riboflavin Drugs 0.000 description 1
- 229960002668 sodium chloride Drugs 0.000 description 1
- XSXSKSKONCDOMZ-UHFFFAOYSA-N sodium;1,3,5-trichloro-1,3,5-triazinane-2,4,6-trione Chemical compound [Na+].ClN1C(=O)N(Cl)C(=O)N(Cl)C1=O XSXSKSKONCDOMZ-UHFFFAOYSA-N 0.000 description 1
- 229960003495 thiamine Drugs 0.000 description 1
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 1
- 229940035339 tri-chlor Drugs 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
- 235000010374 vitamin B1 Nutrition 0.000 description 1
- 239000011691 vitamin B1 Substances 0.000 description 1
- 235000019164 vitamin B2 Nutrition 0.000 description 1
- 239000011716 vitamin B2 Substances 0.000 description 1
- 235000019160 vitamin B3 Nutrition 0.000 description 1
- 239000011708 vitamin B3 Substances 0.000 description 1
- 239000011675 vitamin B5 Substances 0.000 description 1
- 235000009492 vitamin B5 Nutrition 0.000 description 1
- 235000011912 vitamin B7 Nutrition 0.000 description 1
- 239000011735 vitamin B7 Substances 0.000 description 1
- 235000019159 vitamin B9 Nutrition 0.000 description 1
- 239000011727 vitamin B9 Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Images
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D1/00—Detergent compositions based essentially on surface-active compounds; Use of these compounds as a detergent
- C11D1/66—Non-ionic compounds
- C11D1/667—Neutral esters, e.g. sorbitan esters
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/02—Inorganic compounds ; Elemental compounds
- C11D3/04—Water-soluble compounds
- C11D3/044—Hydroxides or bases
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/02—Inorganic compounds ; Elemental compounds
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- C11D3/046—Salts
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/02—Inorganic compounds ; Elemental compounds
- C11D3/04—Water-soluble compounds
- C11D3/06—Phosphates, including polyphosphates
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/02—Inorganic compounds ; Elemental compounds
- C11D3/04—Water-soluble compounds
- C11D3/10—Carbonates ; Bicarbonates
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
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- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/20—Organic compounds containing oxygen
- C11D3/2003—Alcohols; Phenols
- C11D3/2006—Monohydric alcohols
- C11D3/201—Monohydric alcohols linear
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- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
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- C11D3/26—Organic compounds containing nitrogen
- C11D3/28—Heterocyclic compounds containing nitrogen in the ring
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- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
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- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/34—Organic compounds containing sulfur
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Abstract
The invention discloses a nucleic acid pollution scavenger, which comprises B vitamins, sodium hydroxide, magnesium sulfate/zinc sulfate, sodium chloride, potassium chloride, sodium trichloroisocyanuric acid and Na 2 HPO 4 、KH 2 PO 4 Potassium carbonate, tween, ethanol and deionized water. The preparation method of the nucleic acid pollution scavenger comprises the following steps: step one: formation of nucleic acid contaminant removal agent: step two: the nucleic acid pollution scavenger is formed into a form which is easy to contact with the surface or space polluted by nucleic acid, and the form which is easy to contact with the surface or space polluted by nucleic acid comprises soaking in a large volume of aqueous solution, droplet spraying, atomizing or wet melt spraying. The active ingredient molecules of the nucleic acid pollution scavenger of the invention break DNA or RNA by contacting with air to generate active oxygen and the secondary derivative acts on phosphodiester bond, thus having a certain degree of selective scavenging effect, mild working condition to be alkalescent, no component with any toxicity risk, high nucleic acid scavenging efficiency and capability of scavenging polluted nucleic acid in 3-5 minutes.
Description
Technical Field
The invention relates to the technical field of biology, in particular to a nucleic acid pollution scavenger.
Background
At present, PCR and related nucleic acid amplification detection laboratories have nucleic acid pollution with different severity, and the phenomenon of false positive results is extremely common. Ultraviolet irradiation is a widely used method, but it is not effective against nucleic acid contamination in various contamination forms (aerosol contamination, laboratory table contamination, etc.). Meanwhile, comprehensive analysis literature reports and market information show that most of functional components and using modes of the nucleic acid pollution scavenger have obvious defects, such as extremely poor scavenging effect of common 75% ethanol, and the peroxide has the defects of long acting time, low efficiency (more than half an hour) and poor scavenging effect. Although the highly oxidative hypochlorite removal efficiency is relatively high, high concentrations of the hypochlorite are required to be effective, but this is unavoidable for human health. In addition, scavengers such as strongly acidic (pH < 2.5) present a risk of corrosion to the equipment and environment.
Therefore, a nucleic acid contamination scavenger is proposed for purifying or scavenging PCR experimental environment and other solutions of nucleic acid amplified DNA and RNA contamination.
Disclosure of Invention
The present invention is directed to a nucleic acid contaminant removal agent that solves the above-mentioned problems of the prior art.
In order to achieve the above purpose, the present invention provides the following technical solutions:
a nucleic acid pollution scavenger comprises vitamin B, sodium hydroxide, magnesium sulfate/zinc sulfate, sodium chloride, potassium chloride, sodium trichloroisocyanuric acid, na 2 HPO 4 、KH 2 PO 4 The vitamin B group is 0.038-0.76 g/L, preferably 0.038g/L, the magnesium sulfate/zinc sulfate is 0.01-0.4 g/L, preferably 0.01g/L, the potassium carbonate is 0.01-0.19 g/L, preferably 0.01g/L, the sodium hydroxide is 0.001-0.01 g/L, preferably 0.001g/L, the sodium hydroxide is 0.01-0.1 g/L, preferably 0.01g/L, the sodium chloride is 0.001-0.01 g/L, the potassium chloride is 0.001-0.01 g/L, preferably 0.001g/L, the sodium trichloroisocyanuric acid is 0.001-0.01 g/L, preferably the sodium trichloroisocyanuric acid is 0.001g/L, the Na 2 HPO 4 The concentration is 0.01 to 0.1g/L, preferably Na 2 HPO 4 The concentration is 0.01g/L KH 2 PO 4 The concentration is 0.001-0.01 g/L, preferably KH 2 PO 4 The concentration is 0.001g/L, the concentration of potassium carbonate is 0.10-0.5 g/L, the concentration of potassium carbonate is 0.10g/L preferably, the concentration of Tween is 0.0001-0.001 g/L, the concentration of Tween is 0.0001g/L preferably, the concentration of ethanol is 0.01-0.1 g/L preferably, the concentration of ethanol is 0.01g/L.
As a further scheme of the invention: the B vitamins and magnesium sulfate/zinc sulfate and potassium carbonate act together to degrade nucleic acid.
As still further aspects of the invention: the sodium hydroxide, na 2 HPO 4 ，KH 2 PO 4 The pH value range of the nucleic acid scavenger is maintained between 6 and 10 by the potassium carbonate.
As still further aspects of the invention: the sodium chloride, potassium chloride, sodium trichloroisocyanuric acid, tween, ethanol and the like maintain the water content, viscosity and temperature stability of the nucleic acid scavenger system and improve the activity of the secondary derivative of the B vitamins.
As still further aspects of the invention: the preparation method of the nucleic acid pollution scavenger comprises the following steps:
step one: adding fixed amount of B vitamins, sodium hydroxide, magnesium sulfate/zinc sulfate, sodium chloride, potassium chloride, sodium trichloroisocyanuric acid and Na according to the proportion concentration 2 HPO 4 、KH 2 PO 4 Adding potassium carbonate, tween and ethanol into 1L deionized water, and fully mixing and reacting to form the nucleic acid pollution scavenger:
step two: the nucleic acid pollution scavenger is formed into a morphology mode which is easy to contact with the surface or space polluted by the nucleic acid to efficiently degrade the nucleic acid, thereby realizing the nucleic acid pollution scavenging.
As still further aspects of the invention: the morphology of the surface or space susceptible to nucleic acid contamination includes bulk aqueous immersion, droplet spray, atomization, or wet melt blown distribution.
Compared with the prior art, the invention has the beneficial effects that:
1. the active ingredient molecules of the nucleic acid pollution scavenger of the invention break DNA or RNA by contacting with air to generate active oxygen and the secondary derivative acts on phosphodiester bond, thus having a certain degree of selective scavenging effect, mild working condition to be alkalescent, no component with any toxicity risk, high nucleic acid scavenging efficiency and capability of scavenging polluted nucleic acid in 3-5 minutes.
Drawings
FIG. 1 is a graph showing the comparison of the effects of a nucleic acid contaminant removal agent.
FIG. 2 is a graph showing a comparison of curves of a nucleic acid contaminant removal agent.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Referring to fig. 1-2, the inventionIn the illustrative embodiment, a nucleic acid contaminant scavenger comprises vitamin B, sodium hydroxide, magnesium sulfate/zinc sulfate, sodium chloride, potassium chloride, na 2 HPO 4 、KH 2 PO 4 Potassium carbonate, tween, ethanol and deionized water, wherein the concentration of vitamin B group is 0.038 to 0.76g/L, preferably the concentration of vitamin B group is 0.038g/L, the concentration of magnesium sulfate/zinc sulfate is 0.01 to 0.4g/L, preferably the concentration of magnesium sulfate/zinc sulfate is 0.01g/L, the concentration of potassium carbonate is 0.01 to 0.19g/L, preferably the concentration of potassium carbonate is 0.01g/L, the concentration of sodium hydroxide is 0.001 to 0.01g/L, preferably the concentration of sodium hydroxide is 0.001g/L, the concentration of sodium chloride is 0.01 to 0.1g/L, preferably the concentration of sodium chloride is 0.01g/L, the concentration of potassium chloride is 0.001g/L, preferably the concentration of sodium trichloroisocyanurates is 0.001 to 0.01g/L, preferably the concentration of sodium trichloroisocyanurates is 0.001g/L, na 2 HPO 4 The concentration is 0.01 to 0.1g/L, preferably Na 2 HPO 4 The concentration is 0.01g/L KH 2 PO 4 The concentration is 0.001-0.01 g/L, preferably KH 2 PO 4 The concentration is 0.001g/L, the concentration of potassium carbonate is 0.10-0.5 g/L, the concentration of potassium carbonate is 0.10g/L preferably, the concentration of Tween is 0.0001-0.001 g/L, the concentration of Tween is 0.0001g/L preferably, the concentration of ethanol is 0.01-0.1 g/L preferably, the concentration of ethanol is 0.01g/L.
The B vitamins and magnesium sulfate/zinc sulfate and potassium carbonate act together to degrade nucleic acid, and the B vitamins comprise vitamin B1, vitamin B2, vitamin B3, vitamin B5, vitamin B7 and vitamin B9.
Sodium hydroxide, na 2 HPO 4 ，KH 2 PO 4 The pH value range of the nucleic acid scavenger is maintained between 6 and 10 by the potassium carbonate.
Sodium chloride, potassium chloride, sodium trichloroisocyanurate, tween, ethanol, etc. maintain the water content, viscosity, temperature stability of the nucleic acid scavenger system and raise the activity of secondary derivative of B vitamins.
The preparation method of the nucleic acid pollution scavenger comprises the following steps:
step one: adding fixed amount of B vitamins, sodium hydroxide, magnesium sulfate/zinc sulfate, sodium chloride, potassium chloride and trichlor according to the proportion concentrationSodium chloroisocyanuric acid, na 2 HPO 4 、KH 2 PO 4 Adding potassium carbonate, tween and ethanol into 1L deionized water, and fully mixing and reacting to form the nucleic acid pollution scavenger:
step two: the nucleic acid pollution scavenger is formed into a morphology mode which is easy to contact with the surface or space polluted by the nucleic acid to efficiently degrade the nucleic acid, thereby realizing the nucleic acid pollution scavenging.
Forms that are susceptible to contact with surfaces or spaces that are contaminated with nucleic acids include bulk aqueous solution immersion, droplet spray, atomization, or wet melt blown distribution.
Embodiment one:
and (3) soaking the PCR tube polluted by the PCR product and a pipette tip (without a filter element) in the nucleic acid scavenger for 3-5 minutes, washing the residual nucleic acid scavenger with pollution-free water, and detecting to remove the pollution of the PCR product.
Embodiment two:
the nucleic acid scavenger is soaked by clean paper towel or cloth, the surface of the liquid transfer device is wiped for 3 to 5 times, the residual nucleic acid scavenger is wiped by the paper towel or cloth which is wet by pollution-free water, and the pollution of PCR products can be removed through detection.
Embodiment III:
spraying the nucleic acid scavenger onto the surface of the instrument or the experiment table by using a spray can for 3-5 minutes, wiping off the residual nucleic acid scavenger by using a paper towel or a rag wetted by water to remove the pollution of the PCR product, and detecting to remove the pollution of the PCR product.
Embodiment four:
the laboratory space is filled with the atomized spray nucleic acid scavenger, and the spraying amount is less than 10mL/m 3 Closing the door for 3-5 minutes, opening the exhaust system for ventilation, and detecting to remove PCR product pollution.
Although the present invention has been described with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments described, or equivalents may be substituted for elements thereof, and any modifications, equivalents, improvements and changes may be made without departing from the spirit and principles of the present invention.
Claims (6)
1. A nucleic acid pollution scavenger comprises vitamin B, sodium hydroxide, magnesium sulfate/zinc sulfate, sodium chloride, potassium chloride, sodium trichloroisocyanuric acid, na 2 HPO 4 、KH 2 PO 4 Potassium carbonate, tween, ethanol and deionized water, and is characterized in that: the B vitamins have the concentration of 0.038-0.76 g/L, the concentration of magnesium sulfate/zinc sulfate of 0.01-0.4 g/L, the concentration of potassium carbonate of 0.01-0.19 g/L, the concentration of sodium hydroxide of 0.001-0.01 g/L, the concentration of sodium chloride of 0.01-0.1 g/L, the concentration of potassium chloride of 0.001-0.01 g/L, the concentration of sodium trichloroisocyanuric acid of 0.001-0.01 g/L and Na 2 HPO 4 The concentration is 0.01-0.1 g/L, KH 2 PO 4 The concentration is 0.001-0.01 g/L, the concentration of potassium carbonate is 0.10-0.5 g/L, the concentration of Tween is 0.0001-0.001 g/L, and the concentration of ethanol is 0.01-0.1 g/L.
2. The nucleic acid contaminant removal agent of claim 1, wherein: the B vitamins and magnesium sulfate/zinc sulfate and potassium carbonate act together to degrade nucleic acid.
3. The nucleic acid contaminant removal agent of claim 1, wherein: the sodium hydroxide, na 2 HPO 4 ，KH 2 PO 4 The pH value range of the nucleic acid scavenger is maintained between 6 and 10 by the potassium carbonate.
4. The nucleic acid contaminant removal agent of claim 1, wherein: the sodium chloride, potassium chloride, sodium trichloroisocyanuric acid, tween, ethanol and the like maintain the water content, viscosity and temperature stability of the nucleic acid scavenger system and improve the activity of the secondary derivative of the B vitamins.
5. The nucleic acid contaminant removal agent of claim 1, wherein: the preparation method of the nucleic acid pollution scavenger comprises the following steps:
step one: is concentrated according to the proportionAdding fixed amount of B vitamins, sodium hydroxide, magnesium sulfate/zinc sulfate, sodium chloride, potassium chloride, sodium trichloroisocyanuric acid and Na 2 HPO 4 、KH 2 PO 4 Adding potassium carbonate, tween and ethanol into 1L deionized water, and fully mixing and reacting to form the nucleic acid pollution scavenger:
step two: the nucleic acid pollution scavenger is formed into a morphology mode which is easy to contact with the surface or space polluted by the nucleic acid to efficiently degrade the nucleic acid, thereby realizing the nucleic acid pollution scavenging.
6. The nucleic acid contaminant removal agent of claim 5, wherein: the morphology of the surface or space susceptible to nucleic acid contamination includes bulk aqueous immersion, droplet spray, atomization, or wet melt blown distribution.
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| EP2378524A1 (en) * | 2010-04-15 | 2011-10-19 | Cristanini Spa | Decontamination/detoxification composition |
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| CN112812901A (en) * | 2021-01-29 | 2021-05-18 | 北京华瑞康源生物科技发展有限公司 | Reagent capable of efficiently removing nucleic acid pollution in normal-temperature and low-temperature environments and application |
| DE102020002883A1 (en) * | 2020-04-14 | 2021-10-14 | Van Ba Dam | Disinfectant and anti-virus static |
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| WO2002060539A1 (en) * | 2001-02-01 | 2002-08-08 | Becton Dickinson And Company | Surfactant/oxidizing agent solution and methods of use |
| US20090028961A1 (en) * | 2005-04-30 | 2009-01-29 | Multibind Biotec Gmbh | Decontamination solution and its use for denaturation, modification, degradation, solubilisation and removal of proteins, nucleic acid molecules and microorganisms |
| DE102006018983A1 (en) * | 2006-04-25 | 2007-10-31 | Multibind Biotec Gmbh | Decontamination solution, useful for degrading, solubilizing and removing protein and nucleic acid molecule from a solution and surface e.g. culture medium, comprises a vitamin, a metal ion and a surface active compound |
| US20120107415A1 (en) * | 2008-12-18 | 2012-05-03 | Thomas Lisowsky | Combined disinfection and decontamination agent having increased effectiveness |
| EP2378524A1 (en) * | 2010-04-15 | 2011-10-19 | Cristanini Spa | Decontamination/detoxification composition |
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