CN116035253A

CN116035253A - Moisture-preserving coating film with aroma-enhancing function and application of moisture-preserving coating film to cigarettes

Info

Publication number: CN116035253A
Application number: CN202211614393.6A
Authority: CN
Inventors: 胡少东; 段鹍; 顾亮; 高明奇; 田海英; 万纪强; 李国政; 王鹏飞; 杨琦
Original assignee: China Tobacco Henan Industrial Co Ltd
Current assignee: China Tobacco Henan Industrial Co Ltd
Priority date: 2022-12-12
Filing date: 2022-12-12
Publication date: 2023-05-02

Abstract

The invention discloses an application of a moisture-preserving coating film with aroma enhancement function on cigarettes, which comprises the following steps: the composite bacterial liquid, the tobacco polysaccharide solution and the chitosan solution are prepared from the following components in percentage by volume: (2-3): (4-5), the composite bacterial liquid is prepared by mixing streptococcus lactis, bacillus and saccharomycetes according to a certain proportion. The moisturizing coating film can inhibit mixed bacteria by using secondary metabolites of streptococcus lactis, chitosan is a natural harmless antibacterial film, tobacco polysaccharide has moisturizing performance, composite bacterial liquid can interact with the tobacco polysaccharide and the chitosan, mixed bacteria can be better inhibited, the protective film has better protective effect, and bacillus and saccharomycetes metabolites generate aroma substances in the combustion and pyrolysis process of cigarettes, so that the aroma of the cigarettes is increased.

Description

Moisture-preserving coating film with aroma-enhancing function and application of moisture-preserving coating film to cigarettes

Technical Field

The invention relates to the technical field of cigarette processing, in particular to a moisture-preserving coating film with a flavoring function and application of the moisture-preserving coating film to cigarettes.

Background

In recent years, with the upgrading of the consumption of the cigarette market, the requirements of consumers on the quality of cigarettes are increasing. Chinese style cigarettes have the common characteristics of quick water loss of tobacco shreds and poor water holding capacity of cigarettes. The reduced moisture content of the cigarettes can seriously affect the sensory quality of the cigarettes, such as the problems of increased dry feel and spicy feel of smoke, insufficient clean aftertaste of the cigarettes, reduced sweet feel and the like. The Chinese operators are wide, the climate difference is huge in different areas, and the water content of cigarettes in different sales areas is inconsistent, so that the sensory quality is inconsistent; the seasonal variation also causes the fluctuation of the water content of the cigarettes, thereby affecting the stability of the quality of the cigarettes. Therefore, cigarette moisture retention has been a research hotspot in the field of tobacco processing.

At present, the cigarette moisture retention mainly depends on the addition of a moisture retention agent, and the moisture retention agent can effectively maintain the moisture of the cigarette, reduce breakage of the cigarette, improve the processing resistance of the cigarette, stabilize the sensory quality of the cigarette and the like. The commonly used humectant is propylene glycol, glycerin, sorbitol, etc. Researches show that in the cigarette smoking process, partial propylene glycol, glycerol and other moisture-retaining agents can be transferred into the smoke, and partial propylene glycol, glycerol and other moisture-retaining agents enter the smoke to form tar, so that the sensory quality of the cigarette is affected to a certain extent.

The film coating agent is widely applied in the food processing field due to the characteristics of effectively reducing water loss of the film coating object, inhibiting the growth of mixed bacteria and the like. Researches show that the fresh-keeping period of fruits and eggs is obviously prolonged after the film coating agent is added. At present, the application of the film coating agent in the field of cigarette processing is not reported.

Disclosure of Invention

The invention aims to solve the technical problem of providing a moisture-preserving coating film with a flavoring function and application of the moisture-preserving coating film to cigarettes, and provides a new idea for developing a novel green, environment-friendly and effective additive.

The technical problems to be solved by the invention are realized by the following technical scheme:

a moisture-retaining coating film having a flavor enhancing function, comprising: the composite bacterial liquid, the tobacco polysaccharide solution and the chitosan solution are prepared from the following components in percentage by volume: (2-3): (4-5), the composite bacterial liquid is prepared by mixing streptococcus lactis, bacillus and saccharomycetes according to a certain proportion.

Preferably, the volume ratio of the streptococcus lactis to the bacillus to the saccharomycetes is (1-2): (1-2): (1-2).

Preferably, the composite bacterial liquid is prepared by the following method: culturing and activating the streptococcus lactis, the bacillus and the saccharomycetes for 3 generations respectively, performing expansion culture for 24 hours, putting the streptococcus lactis, the bacillus and the saccharomycetes in a centrifuge, centrifuging the mixture under the condition of 2000r/min, taking supernatant, and preparing the supernatant according to a proportion.

Preferably, the tobacco polysaccharide solution is a 1.5% tobacco polysaccharide solution, which is prepared by the following method: taking tobacco leaves as raw materials, putting the crushed raw materials into a triangular flask, adding deionized water, placing the triangular flask into a constant-temperature water bath for hydrolysis, rapidly cooling to room temperature after hydrolysis, carrying out reduced pressure suction filtration treatment after supplementing volatile water, adding absolute ethyl alcohol into concentrated solution for alcohol precipitation, centrifuging and drying, taking precipitate, adding a proper amount of deionized water for dissolving, deproteinizing, taking supernatant, centrifuging and drying again, obtaining precipitate which is tobacco polysaccharide, and adding the tobacco polysaccharide powder into deionized water to prepare 1.5% tobacco polysaccharide solution.

Preferably, the chitosan solution is prepared by the following method: at 45 ℃, 1.5 percent of chitosan and 0.1 percent of glycerol are dissolved in 1L of 1 percent acetic acid solution, and the chitosan and the glycerol are obtained after cooling to normal temperature for standby.

The application of a moisture-preserving coating film with aroma-enhancing function in cigarettes.

Preferably, the moisture-retaining coating is used for being sprayed into cigarette paper, filter rods or cut tobacco to retain moisture and flavor of the cigarettes.

Preferably, the moisture-retaining coating film is sprayed in a proportion of 0 to 0.6% by mass fraction.

A cigarette comprises a moisture-retaining coating film in cigarette paper, filter stick or cut tobacco of the cigarette.

The technical scheme of the invention has the following beneficial effects:

the invention provides a moisture-preserving coating film with a flavor enhancing function, the coating film is composed of streptococcus lactis, saccharomycetes, bacillus, chitosan solution and tobacco polysaccharide solution, secondary metabolites of the streptococcus lactis can inhibit mixed bacteria, chitosan is a natural harmless antibacterial film, the tobacco polysaccharide has moisture-preserving performance, bacterial liquid can interact with the tobacco polysaccharide and the chitosan, the mixed bacteria can be better inhibited, the protective effect of the protective film is better, and the bacillus and saccharomycetes metabolites generate flavor-producing substances in the combustion and pyrolysis process of cigarettes, so that the aroma of the cigarettes is increased.

The invention aims to provide a new idea for developing a novel additive which is green, environment-friendly and effective.

The invention utilizes the secondary metabolite of microorganism to inhibit the mixed bacteria, and combines chitosan to generate a composite coating film, thereby being capable of moisturizing cigarettes and improving the quality of products. The synergistic effect of the microorganism and the chitosan is better.

The moisture-preserving coating has wider application range, can be used on cigarette paper, filter sticks and cut tobacco.

Drawings

Fig. 1 is a graph showing the change of the moisture content of tobacco shreds under a high humidity condition.

Fig. 2 is a graph showing the change of the moisture content of tobacco shreds under a low humidity condition.

Fig. 3 is a graph showing the change of neutral aroma components of tobacco shreds after the film coating agent treatment.

Detailed Description

Various exemplary embodiments of the invention will now be described in detail. It should be noted that: the relative arrangement of the components and steps, numerical expressions and numerical values set forth in these embodiments do not limit the scope of the present invention unless it is specifically stated otherwise.

The moisturizing coating film with the aroma enhancement function is prepared from the raw materials of composite bacterial liquid (multi-strain culture liquid), tobacco polysaccharide and chitosan.

(1) Preparing a composite bacterial liquid:

streptococcus lactis (food grade) was cultivated at 37+2℃using MRS medium, bacillus (Bacillus sp.) LBT1.0007 and Saccharomyces (Saccharomyces sp.) LBT1.0038 (Bacillus and Saccharomyces screens from Yunnan high quality tobacco) were cultivated at 37+2℃using beef extract peptone medium, and all of the above 3 strains were obtained by market. After the strain is activated for 3 generations, the strain is subjected to expansion culture for 24 hours, and is put into a centrifugal machine to be centrifuged under the condition of 2000r/min, and then the supernatant is taken for standby.

(2) Preparing a tobacco polysaccharide solution:

the method comprises the steps of taking high-quality Yunnan tobacco leaves as raw materials, crushing the raw materials after passing through a mesh screen, putting the crushed raw materials into a triangular flask, adding deionized water according to a certain feed-liquid ratio, and placing the mixture into a constant-temperature water bath for hydrolysis. After a period of time, the mixture is cooled to room temperature rapidly, and reduced pressure suction filtration treatment is carried out after the volatile water is complemented. And adding absolute ethyl alcohol into the concentrated solution for alcohol precipitation, and then centrifugally drying. Dissolving the precipitate with deionized water, deproteinizing, centrifuging and drying the supernatant to obtain precipitate, namely tobacco polysaccharide, and adding deionized water into tobacco polysaccharide powder to prepare 1.5% tobacco polysaccharide solution.

(3) Preparation of chitosan solution:

at 45 ℃, 1.5 percent of chitosan and 0.1 percent of glycerol are dissolved in 1L of 1 percent acetic acid solution, and the mixture is cooled to normal temperature for standby.

And after the composite coating is uniformly sprayed into tobacco shreds in a cigarette feeding link, comprehensively evaluating the moistening and flavoring effects of the cigarettes.

Table 1, coating formulation

Respectively spraying film coating agent 1, film coating agent 2 and film coating agent 3 into blank tobacco shred samples according to mass fractions of 0 (control sample), 0.2%, 0.4% and 0.6%, and keeping the temperature and humidity constant (humidity 60)+5% of temperature 25+2 ℃) the cigarettes are balanced for 48 hours, and after the cigarettes are balanced for 120 hours in a constant temperature and humidity room, the tissue expert panelist evaluates the cigarettes to examine the aroma enhancement performance of the film coating agent.

Table 2 sensory quality of cigarettes after spraying film coating agent 1

Table 3 sensory quality of cigarettes after spraying film coating agent 2

Spray quantity	Fragrant quality	Fragrance amount	Concentration of	Irritation (irritation)	Miscellaneous gas	Stiff head	Aftertaste of
								0 (control)	5.5	5.5	6.5	6.0	6.0	6.0	6.0
0.2％	6.4	6.5	6.4	6.5	6.7	6.0	6.5
								0.4％	6.3	6.5	6.3	6.5	6.5	6.0	6.5
0.6％	6.3	6.4	6.5	6.5	6.5	6.0	6.5

Table 4 sensory quality of cigarettes after spraying film coating agent 3

Spray quantity	Fragrant quality	Fragrance amount	Concentration of	Irritation (irritation)	Miscellaneous gas	Stiff head	Aftertaste of
								0 (control)	5.5	5.5	6.5	6.0	6.0	6.0	6.0
0.2％	6.6	6.7	6.4	6.6	6.7	6.0	6.8
								0.4％	6.5	6.6	6.4	6.5	6.5	6.0	6.3
0.6％	6.5	6.5	6.5	6.5	6.5	6.0	6.5

As can be seen from the table, the film coating agent provided by the invention has an obvious improvement effect on the sensory quality of cigarettes, compared with a control sample, the film coating agent has slightly reduced concentration after 3 film coating agents are used, and the indexes such as aroma quality, aroma quantity, irritation, miscellaneous gases, aftertaste and the like of the cigarettes are obviously improved without change of strength.

The influence of different addition amounts on the sensory quality of cigarettes is obvious, the optimal use ratio of the film coating agent 1 is 0.4%, and the optimal use amounts of the film coating agent 2 and the film coating agent 3 are 0.2%.

Respectively placing the balanced cigarettes under the low-humidity condition: temperature 22±1 ℃ and humidity 40±2% and high humidity conditions: and (3) measuring the water content of the cigarettes after 240 hours in an environment with the temperature of 22+/-1 ℃ and the humidity of 70+/-2%, and examining the moisture retention performance of the film coating agent.

Compared with the control sample, the 3 kinds of coating agents have better moisturizing effect in both high-humidity environment and low-humidity environment, and the water content of the coating agents is higher than that of the control sample as shown in fig. 1 and 2. The moisture-keeping effect of the cigarettes can be influenced by different addition amounts, and the moisture-keeping effect of 3 coating agents is optimal when the addition amount is 0.6%. The moisturizing effect of the coating film 3 is best, and the moisturizing effect of the coating film 2 is worst.

GC/MS detection analysis: respectively spraying the film coating agent 1 into blank tobacco shred samples according to the mass fractions of 0 (control sample), 0.2%, 0.4% and 0.6%, and keeping the temperature and humidity constant (humidity 60)+5% of temperature 25+2 ℃) for 168 hours, grinding, adding 40g of tobacco powder into a 1000ml round bottom flask, adding 400ml of deionized water, adding 4 zeolite, connecting the round bottom flask to one end of a simultaneous distillation extraction device, and heating to keep the solution slightly boiling; after adding 100ml of methylene chloride solution into a 200ml conical flask, putting the conical flask into a 60ml water bath kettle, simultaneously distilling and extracting for 4 hours, and adding a proper amount of anhydrous sodium chlorate to dry overnight after acid-base extraction of the solution in the round bottom flask. 100. Mu.l of phenethyl acetate was added as an internal standard solution, and the distillate was concentrated to 1ml and analyzed by GC/MS. The fragrance components were quantified by peak area method (ratio of compound peak to internal standard peak area).

The film coating agent can obviously promote neutral aroma components in the tobacco shreds (shown in figure 3), and is specifically expressed as follows: after the film coating agent is treated, the aldehyde, alcohol, ketone and heterocyclic aromatic substances in the cut tobacco are obviously increased, the aldehyde, alcohol and heterocyclic substances are not obviously changed along with the increase of the spraying amount of the film coating agent, and the ketone is obviously increased along with the increase of the spraying amount of the film coating agent; the change of esters and phenols in the cut tobacco after the film coating agent is treated is not obvious.

Although the present invention has been described with reference to the above embodiments, it should be understood that the present invention is not limited thereto, and that various changes and modifications may be made by those skilled in the art without departing from the spirit and scope of the present invention, and the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A moisture-retaining coating film having a flavor enhancing function, comprising: the composite bacterial liquid, the tobacco polysaccharide solution and the chitosan solution are prepared from the following components in percentage by volume: (2-3): (4-5), the composite bacterial liquid is prepared by mixing streptococcus lactis, bacillus and saccharomycetes according to a certain proportion.

2. The moisturizing coating film with flavor enhancing function according to claim 1, wherein the volume ratio of the streptococcus lactis, the bacillus and the saccharomycete is (1-2): (1-2): (1-2).

3. The moisturizing coating film with aroma enhancement function according to claim 2, wherein the composite bacterial liquid is prepared by the following method: culturing and activating the streptococcus lactis, the bacillus and the saccharomycetes for 3 generations respectively, performing expansion culture for 24 hours, putting the streptococcus lactis, the bacillus and the saccharomycetes in a centrifuge, centrifuging the mixture under the condition of 2000r/min, taking supernatant, and preparing the supernatant according to a proportion.

4. The moisturizing coating film with flavor enhancement function according to claim 1, wherein the tobacco polysaccharide solution is 1.5% of tobacco polysaccharide solution, which is prepared by the following method: taking tobacco leaves as raw materials, putting the crushed raw materials into a triangular flask, adding deionized water, placing the triangular flask into a constant-temperature water bath for hydrolysis, rapidly cooling to room temperature after hydrolysis, carrying out reduced pressure suction filtration treatment after supplementing volatile water, adding absolute ethyl alcohol into concentrated solution for alcohol precipitation, centrifuging and drying, taking precipitate, adding a proper amount of deionized water for dissolving, deproteinizing, taking supernatant, centrifuging and drying again, obtaining precipitate which is tobacco polysaccharide, and adding the tobacco polysaccharide powder into deionized water to prepare 1.5% tobacco polysaccharide solution.

5. The moisturizing coating film with flavor enhancing function according to claim 1, wherein the chitosan solution is prepared by the following method: at 45 ℃, 1.5 percent of chitosan and 0.1 percent of glycerol are dissolved in 1L of 1 percent acetic acid solution, and the chitosan and the glycerol are obtained after cooling to normal temperature for standby.

6. Use of a moisture-retaining film according to any one of claims 1-5 with a flavoring function in cigarettes.

7. The use according to claim 6, wherein the moisture-retaining coating is for spraying into cigarette paper, filter rod or cut tobacco to retain moisture and flavour the cigarette.

8. The use according to claim 7, characterized in that the moisture-retaining coating film is sprayed in a proportion of 0 to 0.6% by mass fraction.

9. A cigarette characterized in that the cigarette paper, filter rod or cut tobacco of the cigarette comprises the moisture-preserving coating film of claims 1-5.