CN116011498A

CN116011498A - Radio frequency counting device

Info

Publication number: CN116011498A
Application number: CN202111517380.2A
Authority: CN
Inventors: 沈海滢; 王志栋; 吴枭; 高运华
Original assignee: National Institute of Metrology
Current assignee: National Institute of Metrology
Priority date: 2021-12-13
Filing date: 2021-12-13
Publication date: 2023-04-25

Abstract

The invention discloses a wireless frequency counting device comprising a detection part and a receiving part, wherein the detection part comprises a fixing frame, a permanent magnet, a Hall sensor, a singlechip, a wireless transmitting module and a power supply battery; the receiving part comprises a wireless receiving module, a serial port conversion module and a terminal. The permanent magnet is fixed on the fixing frame, the Hall sensor is arranged below the permanent magnet, one end of the fixing frame is placed on the magnetic sleeve frame of the nucleic acid mention instrument, when the magnetic sleeve frame moves up and down in a reciprocating mode, the permanent magnet moves up and down along with the magnetic force, the Hall sensor senses the magnetic force change, an electric signal is formed, and the electric signal is transmitted to the singlechip for analysis and counting. And then the singlechip transmits the data to the wireless transmitting module, the wireless transmitting module transmits the signal to a wireless receiving module corresponding to the outside of the nucleic acid extractor, and the serial port conversion module transmits the signal to the computer for data acquisition and analysis. The wireless frequency counting device has the advantages of small volume, portability, wireless data transmission, and online data reading and analysis.

Description

Radio frequency counting device

Technical Field

The invention belongs to the field of molecular biology, in particular to a wireless frequency counting device, and more particularly relates to the field of vibration frequency metering of nucleic acid extraction.

Background

The nucleic acid extractor is an instrument for extracting sample nucleic acid by using matched nucleic acid extracting reagent. Widely applied to various fields such as clinical medicine, preventive medicine, biochemistry and molecular biology, cell biology, environmental science, food science and the like. At present, the dominant nucleic acid extraction method in the market is a magnetic bead method, the method applies a nano technology to modify the surface of superparamagnetism nano particles to prepare superparamagnetism silicon oxide nano magnetic beads which can be specifically identified and efficiently combined with nucleic acid molecules on a microscopic interface, and DNA and RNA can be separated from samples such as blood, animal tissues, food, pathogenic microorganisms and the like under the action of an external magnetic field by utilizing the superparamagnetism of the nano magnetic microspheres coated with silicon dioxide. In the extraction process, the magnetic beads need to fully react with nucleic acid molecules in the solution under the action of a magnetic field, the stability of vibration of the magnetic sleeve frame is one of important performances of the nucleic acid extractor, and the better the stability is, the higher the efficiency and the smaller the uncertainty of nucleic acid extraction are. Therefore, the technique of the vibration frequency of the magnetic rack of the nucleic acid extractor is very important for evaluating the working performance of the instrument.

Currently, existing vibration frequency measurement devices mainly adopt an optical detection mode, such as detection by utilizing the characteristics of scattered light variation and laser displacement variation.

For example, a method for measuring vibration frequency and a vibration frequency measuring device (patent number ZL 2009101274775) are provided, and the invention provides a method for measuring vibration frequency and a technical scheme of the vibration frequency measuring device.

For example, a method for detecting vibration frequency of an electric toothbrush is disclosed (patent application number: 2020109226530), wherein a measuring point is selected on a measured sample, a reflective patch is arranged at the measuring point, then a laser displacement sensor is used for detecting position change of reflected light of the measuring point of the measured sample in vibration by using a triangle measurement method, and the vibration frequency of the measured sample is calculated according to the position change data.

The equipment used in the above patent technology, such as photomultiplier, laser displacement sensor, etc. has higher cost, and the equipment volume or installation volume is great, and the detection space that can provide inside the nucleic acid extraction appearance is less, and inconvenient application is the transmission data of wired mode moreover, and the nucleic acid extraction appearance is at the during operation, and the door of instrument is in closed state, can't use wired equipment to measure.

Disclosure of Invention

The invention aims to provide a wireless frequency counting device which has small volume, is convenient to carry, can wirelessly transmit data and can read and analyze the data on line.

The invention relates to a wireless frequency counting device for nucleic acid detection, which comprises a detection part and a receiving part, wherein the detection part comprises a fixing frame, a permanent magnet (3) fixed at one end of a fixed heater (2), a Hall sensor (4) for super-sensing the change of a magnetic field of the permanent magnet (3) to generate an electric signal, a singlechip for reading the signal transmitted by the Hall sensor (4), a wireless transmitting module and a power supply battery; the receiving part comprises a wireless receiving module, a serial port conversion module and a computer; the fixing frame is placed on a magnetic sleeve frame of the nucleic acid extractor, the reciprocating motion of the magnetic sleeve frame can drive the permanent magnet to move up and down through the fixing frame, the Hall sensor senses the change of the magnetic field of the permanent magnet to generate an electric signal, and the receiving module receives the signal change and displays the frequency value in a terminal such as a computer or a mobile terminal such as a mobile phone.

Further, the reciprocating motion of the magnetic sleeve frame of the nucleic acid extractor drives the permanent magnet to move up and down through the fixing frame, and a changing magnetic field is generated based on the up and down motion.

Further, the Hall sensor has a length of 32mm, a width of 14mm and a thickness of 4mm.

Furthermore, the wireless transmitting module and/or the wireless receiving module each use a built-in antenna of a printed circuit board, and preferably each use a built-in antenna of a printed circuit board, so that the volume of the device is further reduced.

Further, the hall sensor and the wireless transmitting module are embedded in a circuit board, and the circuit board is powered by a power supply battery.

Further, the singlechip processes the data and sends signals to the wireless transmitting module, and the wireless transmitting module transmits the signals to the wireless receiving module corresponding to the outside of the nucleic acid extractor.

A verification device of an invalid frequency counting device for nucleic acid detection comprises a vibration source standard and a wireless frequency counting device for nucleic acid detection, wherein the accuracy of the wireless frequency counting device is verified through frequency comparison of the vibration source standard and the wireless frequency counting device.

The invention provides a wireless frequency counting device of a nucleic acid extractor, which has small volume, is convenient to carry and has verified measurement accuracy. The Hall sensor, the singlechip and the wireless transmission module are small in volume and can be integrated on the surface of a circuit board and placed in the nucleic acid extractor, the Hall sensor is used for measuring, the volume is small, the normal movement of a magnetic sleeve frame of the nucleic acid extractor is not influenced, most of the existing other devices adopt an optical detection mode, the device is large in volume and large in application space requirement, and the device cannot be used in the nucleic acid extractor; the invention has the advantages of small weight, portability and convenience for on-site metering work; the invention uses dry battery or chargeable battery to supply DC power, does not need external power supply, has low requirement on detection site condition, and is convenient to use; the Hall sensor and the wireless transmitting module used in the invention have lower power and are suitable for long-time use in cooperation with frequency detection; the wireless data transmission can realize the detection of closing the bin gate of the nucleic acid extractor, the normal use of the equipment is not affected in the frequency metering process, and the data is read and analyzed on line; in the preferred mode, the single chip microcomputer is adopted to calculate the vibration times of the first 10 seconds every 1 second, so that the frequency of vibration can be accurately read, and the resolution is high. The invention creatively converts the mechanical vibration information of the nucleic acid extractor into magnetic field signals, further converts the magnetic field signals into electric signals, transmits the electric signals to a computer for recording in a wireless communication mode, solves the problem of accurate measurement of the vibration frequency of the nucleic acid extractor, and provides creative ideas for detection and counting of similar vibration frequencies.

Drawings

FIG. 1 is a schematic diagram of a detecting portion of a wireless frequency counting device according to the present invention;

fig. 2 is a schematic diagram of a receiving portion of a radio frequency counting device according to the present invention.

Fig. 3 is a schematic diagram of a detecting portion of a radio frequency counting device according to the present invention.

Fig. 4 is a schematic diagram of the working principle of the present invention.

In the figure: 1. the magnetic sleeve frame comprises a magnetic sleeve frame body, 2, a fixing frame, 3, a permanent magnet, 4, a Hall sensor, 5, a single chip microcomputer, 6, a wireless transmitting module, 7, a power supply battery, 8, a wireless receiving module, 9, a serial port conversion module, 10, a computer, 11, the Hall sensor, 12, the single chip microcomputer, 13, the wireless transmitting module, 14, an integrated circuit board, 15, an electric wire, 16, a battery, 17, a reaction tank, 18, a wireless frequency counting device detecting part, 19, a nucleic acid extracting instrument, 220 and a wireless frequency counting device receiving part.

Detailed Description

The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art without making any inventive effort, based on the embodiments of the present invention are within the scope of the present invention, and the present invention is further described below with reference to the embodiments.

As shown in fig. 1, a radio frequency technology apparatus according to the present invention includes a detecting section and a receiving section. The detection part comprises a fixing frame 2, a permanent magnet 3, a Hall sensor 4, a singlechip 5, a wireless transmitting module 6 and a power supply battery 7. One end of the fixing frame 2 can be placed on the magnetic sleeve frame 1 of the nucleic acid extractor, the other end of the fixing frame is provided with the permanent magnet 3, and when the magnetic sleeve frame 1 of the nucleic acid extractor moves up and down in a reciprocating manner, the permanent magnet 3 is driven by the fixing frame 2 to move up and down along with the magnetic sleeve frame 1 of the nucleic acid extractor, and the frequency is consistent with that of the magnetic sleeve frame 1 of the nucleic acid extractor. The Hall sensor 4 has small module volume, the length is 32mm, the width is 14mm and the thickness is 4mm. The wireless transmitting module 6 adopts a built-in PCB antenna, and the volume is smaller: the length is 24mm, the width is 14mm, and the thickness is 2mm. Both the hall sensor 4 and the wireless transmitting module 6 may be embedded in a circuit board, which is powered by a power supply battery 7, and the power supply battery 7 may be a 3-cell No. 7 battery. The signal transmitted by the hall sensor is read once every millisecond in the program set by the singlechip 5, and accumulated, and the vibration frequency of the first 10 seconds is calculated every 1 second, so that the vibration frequency can be accurately read, and the resolution is 0.1 Hz. The whole detection device has the volume of 55mm (length) ×39mm (width) ×35 (thickness), is small in size, can be placed below the magnetic sleeve frame 1 side of the nucleic acid extraction instrument, and does not influence the closing of the bin gate and the normal operation of the instrument. As shown in fig. 2, the receiving section includes a wireless receiving module 8, a serial port conversion module 9, and a computer 10.

In the working process of the nucleic acid extraction instrument, the magnetic beads need to move up and down under the action of a magnetic field, so that the magnetic beads fully react with nucleic acid molecules in the solution, the magnetic sleeve frame 1 of the nucleic acid extraction instrument vibrates up and down at the moment, meanwhile, the permanent magnet 3 moves up and down along with the magnetic sleeve frame 1 of the nucleic acid extraction instrument under the traction action of the fixing frame 2, the frequency is consistent with that of the magnetic sleeve frame 1 of the nucleic acid extraction instrument, the Hall sensor 4 on the circuit board can detect the change of the magnetic field, and then the information of the change of the magnetic field is converted into an electric signal, the electric signal is transmitted to the singlechip 5 on the circuit board, the singlechip 5 processes the data, sends the signal to the wireless transmitting module 6, and the wireless transmitting module transmits the signal to the wireless receiving module 8 corresponding to the outside of the nucleic acid extraction instrument. After receiving the signal, the wireless receiving module 8 transmits the signal to the serial port conversion module 9, and then transmits the signal to the computer 10 to display the frequency value.

In specific use (see fig. 1-4), one end of the fixing frame 18 can be placed on the magnetic sleeve frame 1 of the nucleic acid extractor 19 of the RDNApruify type Shen Ke in lake, and a permanent magnet 3 is fixed on the other end of the fixing frame 2. The wireless frequency counting device is started to detect partial switch, the battery 16 (3 sections of No. 7 dry batteries) starts to supply power, the device is placed at a proper position right below the permanent magnet 3, the bin gate of the nucleic acid extractor 19 is closed, the vibration mode of the magnetic sleeve frame 1 of the nucleic acid extractor 19 is started, and the frequency value on the screen of the computer 10 is observed. When the device is used, the height of the detection part of the wireless frequency counting device needs to be properly adjusted, so that the Hall sensor can not sense a magnetic field when the permanent magnet vibrates up and down, and can sense the magnetic field when the permanent magnet is at the highest position, and the change of current in a circuit is formed, thereby counting.

The invention provides a method for fixing a permanent magnet on a magnetic sleeve frame of a nucleic acid extraction instrument, wherein a Hall sensor is arranged below the permanent magnet, when the magnetic sleeve frame moves up and down in a reciprocating manner, the permanent magnet moves up and down along with the magnetic force, and the Hall sensor senses the change of the magnetic force so as to form an electric signal, and the electric signal is transmitted to a singlechip for analysis and counting. And then the singlechip transmits the data to the wireless transmitting module, the wireless transmitting module transmits the signal to the wireless receiving module corresponding to the outside of the nucleic acid extractor, and the serial port conversion module transmits the signal to the computer for data acquisition and analysis, and the data is displayed on a computer screen.

The hall sensor is a magnetic field sensor manufactured according to the hall effect, when current passes through a semiconductor perpendicular to an external magnetic field, carriers deflect, and an additional electric field is generated perpendicular to the directions of the current and the magnetic field, so that a potential difference is generated at two ends of the semiconductor, and the phenomenon is the hall effect. The voltage generated by the hall effect varies with the intensity of the magnetic field, the stronger the magnetic field, the higher the voltage, the weaker the magnetic field, the lower the voltage, the smaller the hall voltage value, typically only a few millivolts, but amplified by an amplifier in the integrated circuit can be sufficient to output a stronger signal. Hall sensors developed using this principle have been applied to distributor signal detection, speed detection and various switches.

The Hall sensor module adopted by the invention has the advantages of small volume: 32mm (length) ×14mm (width) ×4mm (thickness), the wireless transmission module built-in PCB antenna that adopts, the volume is also less: 24mm (length) ×14mm (width) ×2mm (thickness), both can imbed in the circuit board, and whole detection device (including 3 No. 7 batteries) is 55mm (length) ×39mm (width) ×35 (thickness), and small in size can place the magnetism cover frame side below of nucleic acid extraction appearance, does not influence the instrument and closes the bin gate and normally work.

And meanwhile, correcting the equipment in order to verify the accuracy of the frequency data extracted by the equipment. The device was calibrated using a vibration source etalon: a permanent magnet is stuck on the surface of a vibrating table of a vibrating source standard device, the device is fixed at a proper position in the vertical direction of the permanent magnet, the power supply of the device is started, and the device starts to work; starting a vibration source standard device, and sequentially adjusting the vibration frequency to 0.1 Hz, 0.5 Hz, 0.7Hz, 1.0 Hz, 5.0 Hz, 10.0 Hz, 20.0 Hz and 50.0 Hz, observing the computer display count, and ensuring that the device has accurate and reliable counting result, wherein the result is shown in Table 1.

TABLE 1 frequency count results

Vibration source standard sets vibration frequency (Hz)	The device measures the frequency (Hz)
		0.1	0.1
0.5	0.5
		0.7	0.7
1.0	1.0
		5.0	5.0
10.0	10.0
		20.0	20.0
50.0	50.0

It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. The present embodiments are, therefore, to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.

Furthermore, it should be understood that although the present disclosure describes embodiments, not every embodiment is provided with a separate embodiment, and that this description is provided for clarity only, and that the disclosure is not limited to the embodiments described in detail below, and that the embodiments described in the examples may be combined as appropriate to form other embodiments that will be apparent to those skilled in the art.

Claims

1. The wireless frequency counting device for detecting the nucleic acid comprises a detection part and a receiving part, wherein the detection part comprises a fixing frame (2), a permanent magnet (3) fixed at one end of the fixing frame (2), a Hall sensor (4) for super-sensing the change of a magnetic field of the permanent magnet (3) to generate an electric signal, a singlechip (5) for reading the signal transmitted by the Hall sensor (4), a wireless transmitting module (6) and a power supply battery (7); the receiving part comprises a wireless receiving module (8), a serial port conversion module (9) and a terminal (10), wherein the serial port conversion module (9) transmits signals received by the wireless receiving module (8) to the terminal (10); when the fixing frame (2) is placed on the magnetic sleeve frame (1) of the nucleic acid extraction instrument, the reciprocating motion of the magnetic sleeve frame (1) can drive the permanent magnet (3) to move up and down through the fixing frame (2), the Hall sensor (4) senses the change of the magnetic field of the permanent magnet to generate an electric signal, and the receiving module (8) receives the signal change and displays the frequency value at the terminal.

2. The wireless frequency counting device for detecting nucleic acid according to claim 1, wherein: the reciprocating motion of the magnetic sleeve frame (1) drives the permanent magnet (3) to move up and down through the fixing frame (2), and a changing magnetic field is generated based on the up and down motion.

3. The wireless frequency counting device for detecting nucleic acid according to claim 2, wherein: the length of the Hall sensor (4) is 32mm, the width is 14mm, and the thickness is 4mm.

4. The wireless frequency counting device for detecting nucleic acid according to claim 3, wherein: the Hall sensor (4) and the wireless transmitting module (6) are embedded in a circuit board, and the circuit board is powered by a power supply battery (7).

5. The wireless frequency counting device for detecting nucleic acid according to claim 1, wherein: the wireless transmitting module (6) and/or the wireless receiving module (8) use a printed circuit board built-in antenna.

6. The wireless frequency counting device for detecting nucleic acid according to claim 4, wherein: the single chip microcomputer (5) processes the data and sends signals to the wireless transmitting module (6), and the wireless transmitting module (6) transmits the signals to the wireless receiving module (8) corresponding to the outside of the nucleic acid extractor.

7. The wireless frequency counting device for detecting nucleic acid according to claim 1, wherein: the terminal is a computer, or a mobile terminal.

8. A verification device for an invalid frequency counting device for detecting nucleic acid, comprising a vibration source standard and the wireless frequency counting device for detecting nucleic acid according to any one of claims 1 to 7, wherein the accuracy of the wireless frequency counting device is verified by comparing the frequency of the vibration source standard with the frequency of the wireless frequency counting device.