CN115990226A - Polygonatum sibiricum powder for improving active ingredients in rhizoma polygonati, and processing method and application thereof - Google Patents
Polygonatum sibiricum powder for improving active ingredients in rhizoma polygonati, and processing method and application thereof Download PDFInfo
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
The present disclosure relates to a sealwort powder for increasing active ingredients in sealwort, a processing method and uses thereof, the method comprising: s1: freeze-drying rhizoma Polygonati slices at low temperature, and pulverizing to obtain first rhizoma Polygonati powder; s2: the first rhizoma polygonati powder is subjected to high-temperature and high-pressure treatment to obtain rhizoma polygonati powder; the conditions of the high-temperature high-pressure treatment include: the temperature is 105-136 ℃, and the pressure is 0.1-0.25 Mpa. The rhizoma Polygonati powder prepared by the method has high active ingredient, and is simple, safe and sanitary.
Description
Technical Field
The present disclosure relates to the field of important processing technologies, and in particular, to a rhizoma Polygonati powder for improving active ingredients in rhizoma Polygonati, and a processing method and use thereof.
Background
Rhizoma polygonati is a medicinal and edible plant resource, is called as 'blood and qi tonifying king' in traditional Chinese medicine, and is the widest in planting range in rhizoma polygonati plants, and has the largest market share. Liu Shuang et al in the research progress of chemical composition and pharmacological action of Polygonatum sibiricum Red describe that the active ingredients of Polygonatum sibiricum Red are mainly polysaccharides, steroid saponins, triterpene saponins, flavones and anthraquinones.
The traditional processing method of rhizoma Polygonati is "nine steaming and nine sun drying", also called nine-processed rhizoma Polygonati, has reduced irritation after processing and can be stored for a long period. Zhang Ying et al in research on influence of processing on chemical components and pharmacological actions of Polygonatum sibiricum Red, describe that chemical components and pharmacological activities are greatly changed before and after processing; sea Mei Rong in the research on chemical composition and biological activity of Polygonatum cyrtonema Fabricius after "Jiuzhu Zhu" of Polygonatum cyrtonema Falcatum, the clinical activity of Polygonatum cyrtonema Falcatum for treating diabetes is relatively greatly improved.
However, the preparation method of the nine-made rhizoma polygonati is complex, the process for producing the nine-made rhizoma polygonati in different factories has no unified standard, and the safety and the stability are not clear, so that the industrial popularization of the nine-made rhizoma polygonati is not facilitated.
Disclosure of Invention
The purpose of the present disclosure is to provide a rhizoma polygonati powder for improving active ingredients in rhizoma polygonati, a processing method and application thereof, wherein the rhizoma polygonati powder prepared by the method has higher active ingredients, and the method is simple, safe and sanitary.
In order to achieve the above object, a first aspect of the present disclosure provides a processing method for improving active ingredients in rhizoma Polygonati, the method comprising:
s1: performing low-temperature freeze drying treatment on the rhizoma polygonati slices, and then performing crushing treatment to obtain first rhizoma polygonati powder;
s2: the first rhizoma polygonati powder is subjected to high-temperature and high-pressure treatment to obtain rhizoma polygonati powder;
the conditions of the high-temperature high-pressure treatment include: the temperature is 105-136 ℃, and the pressure is 0.1-0.25 Mpa.
Optionally, the conditions of the high-temperature high-pressure treatment include: the temperature is 105-135 ℃ and the pressure is 0.1-0.2 Mpa.
Optionally, the high temperature and high pressure treatment conditions further include: the time is 1-10 h.
Optionally, in step S1, the conditions of the low-temperature freeze-drying process include: the temperature is between minus 55 ℃ and minus 40 ℃ and the time is between 72 and 96 hours.
Optionally, the processing method further comprises: cleaning rhizoma Polygonati tubers, and slicing to obtain rhizoma Polygonati slices.
Optionally, the rhizoma polygonati tablet has a thickness of 1-4 mm.
Optionally, the particle size of the rhizoma polygonati powder is 70-100 mu m.
A second aspect of the present disclosure provides a sealwort powder prepared by the method of the first aspect.
Optionally, the content of flavonoids in the rhizoma polygonati powder is 0.2-3.1 mg/g, the content of total saponins is 110.9-120.3 mg/g, the content of total phenols is 1.8-15.6 mg/g, and the content of total polysaccharides is 360-910.5 mg/g.
A third aspect of the present disclosure provides use of the sealwort powder of the first and second aspects in the preparation of an anti-inflammatory medicament.
Through the technical scheme, the disclosure provides the rhizoma polygonati powder for improving the active ingredients in the rhizoma polygonati and the processing method thereof, the rhizoma polygonati powder prepared by the method has higher active ingredients, can achieve better anti-inflammatory effect, is safe and sanitary, and is suitable for standardized production.
Additional features and advantages of the present disclosure will be set forth in the detailed description which follows.
Drawings
The accompanying drawings are included to provide a further understanding of the disclosure, and are incorporated in and constitute a part of this specification, illustrate the disclosure and together with the description serve to explain, but do not limit the disclosure. In the drawings:
FIG. 1 is a graph of flavonoid content for blank samples and samples B, C, D, and E.
FIG. 2 is a graph of total phenol content for blank samples and samples B, C, D, and E.
FIG. 3 is a graph showing the total saponins content of blank samples and samples B, C, D and E.
FIG. 4 is a graph showing the total polysaccharide content of blank samples and samples B, C, D and E.
Fig. 5 is a graph of NO production of blank samples and samples B, C, D, and E.
Detailed Description
Specific embodiments of the present disclosure are described in detail below with reference to the accompanying drawings. It should be understood that the detailed description and specific examples, while indicating and illustrating the disclosure, are not intended to limit the disclosure.
A first aspect of the present disclosure provides a processing method for improving an active ingredient in rhizoma Polygonati, the method comprising: s1: freeze-drying rhizoma Polygonati slices at low temperature, and pulverizing to obtain first rhizoma Polygonati powder; s2: the first rhizoma polygonati powder is subjected to high-temperature and high-pressure treatment to obtain rhizoma polygonati powder; the conditions of the high-temperature high-pressure treatment include: the temperature is 105-136 ℃, and the pressure is 0.1-0.25 Mpa.
In a preferred embodiment of the present disclosure, the conditions of the high temperature and high pressure treatment include: the temperature is 105-135 ℃ and the pressure is 0.1-0.2 Mpa. In one embodiment of the present disclosure, the conditions of the high temperature and high pressure treatment further include: the time is 1-10 h.
In the method, the rhizoma polygonati is processed in a high-temperature and high-pressure mode, so that the temperature and pressure can be accurately controlled; further, the compounds in the rhizoma polygonati are subjected to sugar chain breakage, maillard reaction, caramelization reaction and the like by high-temperature and high-pressure treatment, so that new compounds are generated, and the active ingredients of the compounds are further improved.
In one embodiment of the present disclosure, in step S1, the conditions of the low-temperature freeze-drying process include: the temperature is between 55 ℃ below zero and 40 ℃ below zero for 72 to 96 hours.
In the method, the active ingredients in the rhizoma polygonati can be more reserved by adopting low-temperature freeze drying treatment.
In a specific embodiment of the present disclosure, in step S1, the conditions of the pulverizing treatment include: rapidly crushing the rhizoma Polygonati slices after low-temperature drying with a crusher for 1-2min, and sieving the crushed sample with a 200-mesh sieve. The breaker used may be commercially available, for example, from Tianjin Test instruments Inc. under the trade designation FW177.
In one embodiment of the present disclosure, the processing method further includes: cleaning rhizoma Polygonati tubers, and slicing to obtain rhizoma Polygonati slices; optionally, the rhizoma polygonati tablet has a thickness of 1-4 mm.
In one embodiment of the present disclosure, the particle size of the sealwort powder may vary within a wide range, and the particle size of the sealwort powder may be 70-100 μm.
A second aspect of the present disclosure provides a sealwort powder prepared by the method of the first aspect.
In one specific embodiment of the disclosure, the content of flavonoids in the rhizoma polygonati powder is 0.2-3.1 mg/g, the content of total saponins is 110.9-120.3 mg/g, the content of total phenols is 1.8-15.6 mg/g, and the content of total polysaccharides is 360-910.5 mg/g.
A third aspect of the present disclosure provides use of the sealwort powder of the first and second aspects in the preparation of an anti-inflammatory medicament.
The invention is further illustrated below in connection with specific embodiments, but the scope of the invention as claimed is not limited to the examples described.
The chemicals used in each example were commercially available from public sources.
The kit for measurement is purchased from Suzhou dream rhinoceros biomedical technology Co.
The low temperature freeze drying process in this disclosure is performed using a conventional low temperature freeze dryer.
The model of the pulverizer adopted in the present disclosure is: tianjin Test instruments Co., ltd. FW177.
The model of the rotary evaporator that adopts in this disclosure is: rotary evaporator RE-52AA of Shanghai asia biochemical instrumentation factory.
Example 1
Sample pretreatment: cleaning tuber of Polygonatum kingianum, slicing, freeze-drying at-50deg.C for 72 hr, and processing in pulverizer to obtain first Polygonatum kingianum powder;
high-temperature and high-pressure treatment: placing the first Polygonatum kingianum powder into a high temperature high pressure sterilizing pot, and treating at 105deg.C under 0.1Mpa for 2 hr to obtain Polygonatum kingianum powder B.
Example 2
Sample pretreatment: cleaning tuber of Polygonatum kingianum, slicing, freeze-drying at-50deg.C for 72 hr, and processing in pulverizer to obtain first Polygonatum kingianum powder;
high-temperature and high-pressure treatment: placing the first Polygonatum kingianum powder into a high temperature high pressure sterilizing pot, and treating at 120deg.C under 0.5Mpa for 2 hr to obtain Polygonatum kingianum powder C.
Example 3
Sample pretreatment: cleaning tuber of Polygonatum kingianum, slicing, freeze-drying at-50deg.C for 72 hr, and processing in pulverizer to obtain first Polygonatum kingianum powder;
high-temperature and high-pressure treatment: placing the first Polygonatum kingianum powder into a high temperature high pressure sterilizing pot, and treating at 135deg.C under 0.25Mpa for 2 hr to obtain Polygonatum kingianum powder D.
Comparative example 1
Cutting rhizoma Polygonati Odorati tuber, steaming for 2 hr, and air-drying for 3 days. Repeatedly steaming and sun-drying for nine times to obtain nine-processed rhizoma Polygonati;
drying rhizoma Polygonati in a low temperature freeze dryer;
and (3) crushing the dried sample of the nine-process Polygonatum sibiricum Red in a crusher to obtain powder of the nine-process Polygonatum sibiricum Red, drying the powder of the nine-process Polygonatum sibiricum Red in a drying box at 100 ℃ until the weight of the powder of the nine-process Polygonatum sibiricum Red is constant to obtain a sample E of the nine-process Polygonatum sibiricum Red, drying the sample E of the nine-process Polygonatum sibiricum Red in the drying box at 100 ℃ until the weight of the sample E of the nine-process Polygonatum sibiricum Red is constant, and calculating the moisture content of the sample E of the nine-process Polygonatum sibiricum Red and the original sample of the nine-process Polygonatum sibiricum red, wherein the results are shown in table 1.
Moisture content= (weight before drying-weight after drying)/weight before drying×100%.
TABLE 1
| Weight before drying (g) | Weight after drying (g) | Moisture content (%) | |
| Rhizoma Polygonati is as received | 2 | 1.7609 | 11.955 |
| |
2 | 1.65428 | 17.286 |
Test example 1
The test examples are used for explaining the detection of the content of the active ingredient in the sample B, the sample C, the sample D and the sample E.
Sample pretreatment:
weighing 2g of a sample B, taking an aqueous solution of 80% ethanol as an extracting solution, adding 60mL (namely, the feed liquid ratio=1:3) of 80% ethanol into the sample A, placing the sample A into a rotary evaporator, extracting for 120min at 60 ℃, collecting a supernatant, extracting three times, mixing the supernatants of the three times, and freeze-drying;
pretreatment of the sample C, the sample D and the sample E is the same as that of the sample B; the blank control is Polygonatum kingianum powder (KB) without high temperature and high pressure treatment; the results are shown in Table 2.
TABLE 2
| Sample weight (g) | Weight of lyophilized extract (g) | Extraction yield (%) | |
| |
2 | 0.753 | 37.65 |
| |
2 | 0.852 | 42.6 |
| |
2 | 0.7962 | 39.81 |
| |
2 | 0.512 | 25.6 |
| |
2 | 0.9244 | 46.22 |
The detection of the flavonoid, the total saponins, the total phenols and the total polysaccharide content of the active ingredients adopts a kit, and the calculation formula is as follows:
flavonoid standard curve: y=2.0939x+0.0183, r 2 =0.9991, x is standard concentration (mg/mL), y is Δa, flavonoid content calculation formula (mg/g dry weight) = (Δa-0.0183)/(2.0939) (W/V-like total) = 0.4776 × (Δa-0.0183)/(W), where V-like total: adding 10mL of extract liquid volume; w: sample mass (2 g); Δa=a measured as-a blank, a being the absorbance measured at 25 ℃, 510 nm.
The total saponin content is determined by taking ginsenoside Re as a reference substance; the standard curve regression curve is: y= 2.0466x-0.0016, r 2 =0.9983, x is control concentration (mg/mL), y is Δa, total saponin content (μg/g dry weight) = (Δa+0.0016)/(2.0466 ×v sample/(V sample total×w) ×1000, wherein V sample: add sample volume (0.002 mL); sample V total: adding the volume of the extract (10 mL); w: sample dry weight (2 g); Δa=a measurement-a control, a being the absorbance measured at 25 ℃, 550 nm.
Total phenol content measurement standard curve: y=2.808x+0.0012, r 2 =0.9994, x is standard concentration (mg/mL), y is Δa, total phenol content (mg/g dry weight) = (Δa-0.0012)/(2.808×v sample/(V sample total×w) =0.356× (Δa-0.0012)/(W), where V sample total: adding the volume of the extract (10 mL); v sample: sample volume in reaction (0.01 mL); w: sample mass (2 g); Δa=a measurement-a control, a being the absorbance measured at 25 ℃, 765 nm.
The total polysaccharide content is measured by taking glucose as a reference substance, and a regression equation is measured under standard conditions as y=8.4038x+0.0099; r is R 2 =0.9968, x is glucose content (mg/mL), y is Δa, total polysaccharide content(μg/g dry weight) = (Δa-0.0099)/(8.4038 ×v1)/v2×v3/(wx1000= 594.97 × (Δa-0.0099)/(W), where V1: volume after redissolution after alcohol precipitation (1 mL); v2: volume subjected to alcohol precipitation (0.2 mL); v3, adding the volume (10 mL) of the extracting solution during the extraction; w: sample mass (2 g); 1000 is a conversion factor of mg to μg; Δa=a-a blank was measured, a being the absorbance of each tube measured at 25 ℃ at 490 nm.
The results are shown in fig. 1-4 ("x" represents that the difference in contrast to the other groups and the KB group is very significant, p <0.001; ". Times." represents that the contrast between the rest of the group and KB group is very significant, p <0.01; ". DELTA.DELTA." represents that the contrast between the rest of the group and 9-1 group is very significant, p <0.001; ". DELTA." represents that the contrast between the rest of the group and 9-1 group is very significant, and p < 0.01); KB. Sample B, sample C, sample D, sample E active ingredient contents are as shown in table 3:
TABLE 3 Table 3
As shown in fig. 1, the flavonoid test results showed that the flavonoid content in sample C was 29.0 times that in KB and the flavonoid content in sample D was 75.3 times that in KB; the flavonoid content in sample C was 2.7 times that in sample E, and the flavonoid content in sample D was 7 times that in sample E.
As shown in fig. 2, the total phenol detection result shows that the total phenol content in sample B is 3.5 times that in KB, the total phenol content in sample C is 8.5 times that in KB, and the total phenol content in sample D is 8.7 times that in KB; the total phenol content in sample C was 2.5 times that in E and the total phenol content in sample D was 2.6 times that in E.
As shown in fig. 3, the total saponin detection result shows that the total saponin content in sample D is 1.5 times that in KB; the total saponin content in sample C was 1.1 times that in E and the total saponin content in sample D was 1.6 times that in E.
As shown in fig. 4, the total polysaccharide content in sample B was 1.6 times that in KB, and the total polysaccharide content in sample C was 1.2 times that in KB; the total polysaccharide content in sample B was 2.9 times that in E and the total polysaccharide content in sample C was 2.2 times that in E.
Test example 2
The test examples are used for explaining, detecting the anti-inflammatory activity of the RAW264.7 cells of the sample B, the sample C, the sample D and the sample E.
The pretreatment of this test example was the same as that of test example 1.
Preparation of the drug: dissolving a sample KB, a sample B, a sample C, a sample D and a sample E into 400mg/mL mother liquor respectively by using DMSO; the concentration of the administration treatment cells is 500 mug/mL; the positive control drug was Dexamethasone (DEX) at a concentration of 5 μg/mL; the blank is DMSO.
Cell culture and dosing time: lipopolysaccharide (LPS) for modeling inflammatory injury with concentration of 2 μg/mL; the administration time was 120min.
Inhibition of NO production represents a strong or weak antioxidant activity of the drug against RAW cells, and the lower the NO content, the stronger the antioxidant activity. As a result, as shown in FIG. 5, sample C had a lower NO content than KB and sample E, and sample C had a higher antioxidant activity than KB and sample E, with sample D having the strongest antioxidant activity.
The preferred embodiments of the present disclosure have been described in detail above with reference to the accompanying drawings, but the present disclosure is not limited to the specific details of the above embodiments, and various simple modifications may be made to the technical solutions of the present disclosure within the scope of the technical concept of the present disclosure, and all the simple modifications belong to the protection scope of the present disclosure.
In addition, the specific features described in the foregoing embodiments may be combined in any suitable manner, and in order to avoid unnecessary repetition, the present disclosure does not further describe various possible combinations.
Moreover, any combination between the various embodiments of the present disclosure is possible as long as it does not depart from the spirit of the present disclosure, which should also be construed as the disclosure of the present disclosure.
Claims (10)
1. A processing method for improving active ingredients in rhizoma polygonati, which is characterized by comprising the following steps:
s1: performing low-temperature freeze drying treatment on the rhizoma polygonati slices, and then performing crushing treatment to obtain first rhizoma polygonati powder;
s2: the first rhizoma polygonati powder is subjected to high-temperature and high-pressure treatment to obtain rhizoma polygonati powder;
the conditions of the high-temperature high-pressure treatment include: the temperature is 105-136 ℃, and the pressure is 0.1-0.25 Mpa.
2. The processing method according to claim 1, wherein the conditions of the high-temperature high-pressure treatment include: the temperature is 105-135 ℃ and the pressure is 0.1-0.2 Mpa.
3. The processing method according to claim 2, wherein the high-temperature high-pressure processing conditions further include: the time is 1-10 h.
4. The processing method according to claim 1, wherein in step S1, the conditions of the low-temperature freeze-drying process include: the temperature is between minus 55 ℃ and minus 40 ℃ and the time is between 72 and 96 hours.
5. The processing method according to claim 1, wherein the processing method further comprises: cleaning rhizoma Polygonati tubers, and slicing to obtain rhizoma Polygonati slices.
6. The processing method according to claim 5, wherein the thickness of the rhizoma Polygonati sheet is 1-4 mm.
7. The processing method according to claim 1, wherein the particle size of the rhizoma Polygonati powder is 70-100 μm.
8. The sealwort powder prepared by the method according to any one of claims 1-7.
9. The sealwort powder according to claim 8, wherein the sealwort powder has a flavonoid content of 0.2-3.1 mg/g, a total saponins content of 110.9-120.3 mg/g, a total phenols content of 1.8-15.6 mg/g, and a total polysaccharide content of 360-910.5 mg/g.
10. Use of the sealwort powder according to any one of claims 1-7 and claim 8 for the manufacture of an anti-inflammatory medicament.
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