CN115970914A - Centrifugal equipment and method for detecting cell protein by PCR Array technology - Google Patents
Centrifugal equipment and method for detecting cell protein by PCR Array technology Download PDFInfo
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- CN115970914A CN115970914A CN202310181179.4A CN202310181179A CN115970914A CN 115970914 A CN115970914 A CN 115970914A CN 202310181179 A CN202310181179 A CN 202310181179A CN 115970914 A CN115970914 A CN 115970914A
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Abstract
The invention discloses centrifugal equipment and a method for detecting cell protein by using a PCR Array technology, belonging to the technical field of centrifugal equipment. A centrifugal device for detecting cell protein by using a PCR Array technology comprises a box body and a box cover connected to the top of the box body through a hinge, wherein a fixed plate is fixedly arranged in the box body, a rotating plate is rotatably connected in the fixed plate, a placing groove which is uniformly distributed in the circumference is formed in the rotating plate, a placing frame is arranged in the placing groove, a test tube is placed on the placing frame, a driving rod is fixedly connected to the rotating plate, driving equipment for driving the driving rod to rotate is arranged in the box body, a shock absorption assembly is arranged on the outer side of the driving equipment, and a self-positioning assembly matched with the shock absorption assembly is further arranged on the placing frame; the test tube placing device effectively avoids the situation that the test tube is damaged due to overlarge vibration force, ensures the stability of the use of the test tube, can quickly realize the placing and taking of the test tube in the test tube position, and greatly improves the centrifugal efficiency and the centrifugal effect.
Description
Technical Field
The invention relates to the technical field of centrifugal equipment, in particular to centrifugal equipment and a centrifugal method for detecting cell protein by using a PCR Array technology.
Background
The centrifugal technology is one of the most common methods for separating proteins, enzymes, nucleic acids and cell subcomponents, and is also a separation, purification or clarification method commonly used in biochemical laboratories, and the centrifugal technology is to utilize a strong centrifugal force generated when an object rotates at a high speed to settle or float suspended particles placed in a rotating body so as to concentrate or separate some particles from other particles, wherein the suspended particles are cells, organelles, viruses, biological macromolecules and the like which are made into a suspended state.
When the existing cell centrifuge is used, vibration and noise are generated due to high-speed rotation of a motor, a test tube may be damaged due to overlarge vibration force in a centrifugation process, the test tube needs to be manually fixed and limited to avoid flying out of the test tube in the centrifugation process, so that the operation is complicated when the test tube is taken and placed, and the taking and placing efficiency of the test tube is influenced; simultaneously because current centrifuge generally sets up the standing groove of placing the test tube on the workstation, the standing groove is darker, and the outer hourglass size of the relative standing groove of test tube is less, and the general slope of standing groove sets up, influences the staff to the speed of transferring and the time of taking out of test tube, and then influences centrifugal efficiency.
Disclosure of Invention
The invention aims to solve the problems in the prior art and provides centrifugal equipment and a centrifugal method for detecting cell protein by using a PCR Array technology.
In order to achieve the purpose, the invention adopts the following technical scheme:
the utility model provides a PCR Array technique is at cell protein centrifugal device for detection, includes box and hinged joint at the case lid at box top, the fixed plate has set firmly in the box, the fixed plate internal rotation is connected with the rotor plate, be provided with the standing groove that is circumference evenly distributed on the rotor plate, be provided with the rack in the standing groove, the test tube has been placed on the rack, fixedly connected with actuating lever on the rotor plate, be provided with in the box and be used for driving actuating lever pivoted drive device, the drive device outside is provided with inhales the shake subassembly, still be provided with on the rack and inhale shake subassembly matched with from locating component.
Preferably, the vibration absorption assembly comprises a shell which is fixedly arranged inside the box body and is wrapped outside the driving device, the inner wall of the shell is rotatably connected with a rotating pipe, a storage cavity is formed between the shell and the rotating pipe, liquid is filled in the storage cavity, the rotating pipe is fixedly connected with the driving rod, a plurality of connecting plates are arranged outside the rotating pipe, a first pneumatic telescopic rod is connected onto the connecting plates, and one end, far away from the connecting plates, of the first pneumatic telescopic rod is connected with a stressed plate.
Preferably, the self-positioning assembly comprises an ear plate fixedly arranged on the placing frame, a movable groove is formed in the ear plate, a second pneumatic telescopic rod is arranged on the inner wall of the movable groove, one end, far away from the inner wall of the movable groove, of the second pneumatic telescopic rod is connected with a limiting clamping block, and an extrusion inclined plane is arranged on the limiting clamping block.
Preferably, the first pneumatic telescopic rod and the second pneumatic telescopic rod are the same in structure and comprise sleeves, the sleeves are respectively connected with the connecting plate and the inner wall of the movable groove, each sleeve is connected with a piston in a sliding mode, a second elastic element is arranged between the piston and the inner wall of the sleeve, and one side, deviating from the second elastic element, of the piston is connected with a movable rod.
Preferably, the self-positioning assembly further comprises an air guide groove formed in the connecting plate, the driving rod and the rotating plate, one end of the air guide groove is connected with the first pneumatic telescopic rod, and the other end of the air guide groove is connected with the second pneumatic telescopic rod through a hose.
Preferably, the rack includes sliding connection's upper tube body and body down, be provided with on the body down and be used for the gliding holding tank of upper tube body, be provided with first elastic element between the bottom of holding tank inner wall and upper tube body.
Preferably, the outer side of the upper pipe body and the outer side of the lower pipe body are respectively and rotatably connected with a first ring body and a second ring body, the first ring body is rotatably connected in the placing groove through a pin shaft, a swing rod is movably connected to the second ring body, one end of the swing rod, far away from the second ring body, is movably connected with a moving ring, the moving ring is sleeved on the outer side of the driving rod, a third elastic element is further sleeved on the outer side of the driving rod, two ends of the third elastic element are respectively connected with the moving ring and the shell, a connecting rod is connected to the outer side of the moving ring, a sliding block is fixedly connected to the connecting rod, a sliding chute for sliding the sliding block is formed in the box body, and a pull rope is connected between the sliding block and the box cover.
Preferably, the inner wall of the lower pipe body is provided with a rubber pad, a bottom cavity and a side cavity are formed in the rubber pad, and a communication groove is formed between the bottom cavity and the side cavity.
Preferably, the fixed bevel gear is fixedly connected to the fixed plate, and the rack is provided with a driven bevel gear in meshed connection with the fixed bevel gear.
A centrifugal method for detecting cell protein by using a PCR Array technology comprises the following steps:
s1: when the device is used, the box cover is opened, the box cover is turned relative to the box body, a pulling force is generated on a pulling rope when the box cover is turned, the pulling rope drives the sliding block to move upwards in the sliding groove, the sliding block drives the moving ring to move upwards outside the driving rod through the connecting rod, the third elastic element is stretched, the second ring body is pulled through the swinging rod in the moving-up process of the moving ring, the second ring body drives the lower tube body to move upwards relative to the upper tube body, the first elastic element is compressed, the second ring body drives the lower tube body to rotate by taking the connecting pin shaft of the first ring body and the placing groove as the circle center, the placing rack and the placing groove are in a vertical state, and then test tubes are respectively installed in different placing racks;
s2: then the box cover is turned over, the box cover is closed again at the top of the box body, the box cover does not produce tension on the pull rope any more, the moving ring is reset and moves downwards under the pulling of the elastic force of the third elastic element, the moving ring drives the second ring body to move through the swinging rod, the second ring body drives the lower tube body to deflect relative to the placing groove, the lower tube body slides relative to the upper tube body, the placing rack can contain test tubes with larger area, and the area of the test tubes leaking out of the placing rack is reduced, so that the protection and fixing effects on the test tubes are improved;
s3: the driven bevel gear on the outer side of the deflected placing rack is meshed with the fixed bevel gear on the fixed plate, and when the driving rod drives the placing rack to rotate through the rotating plate, the driven bevel gear on the placing rack can be meshed with the fixed bevel gear and then autorotates;
s4: then controlling the driving device to operate, so that the output end of the driving device drives the driving rod to rotate, and the driving rod drives the placing rack at the placing groove to rotate through the rotating plate, so as to carry out centrifugal operation on the cells in the test tube;
s5 the method comprises the following steps: when the driving rod rotates, the driving rod drives the connecting plate to rotate in the shell through the rotating pipe, the damping characteristic of liquid is utilized to buffer and absorb vibration, the noise is reduced and the vibration is reduced when the driving rod rotates, the risk that the test tube is damaged due to overlarge vibration force in the centrifugal process is reduced, the connecting plate drives the stress plate to rotate in the liquid through the first pneumatic telescopic rod, the stress plate extrudes the first pneumatic telescopic rod under stress, air in the first pneumatic telescopic rod enters the second pneumatic telescopic rod through the air guide groove and the hose, the second pneumatic telescopic rod stretches and pushes the limiting clamping block to move in the movable groove, the limiting clamping block pushes the top of the test tube in the placing groove, and the top of the test tube is positioned;
s6: in-process that the spacing fixture block pushed the test tube, the test tube oppresses the bottom chamber of rubber pad in to lower body, and the air in the bottom chamber passes through the intercommunication groove and gets into the side chamber, and the inflation of side chamber makes the rubber pad oppress the test tube lateral wall, fixes the test tube high efficiency in the centrifugation.
Compared with the prior art, the invention provides centrifugal equipment and a method for detecting cell protein by using a PCR Array technology, and the centrifugal equipment and the method have the following beneficial effects:
1. this PCR Array technique is at cell protein centrifugal equipment and method for measuring, through when the actuating lever rotates, the actuating lever passes through the rotating tube and drives the connecting plate rotatory in the casing, utilize the damping characteristic buffering of liquid self to inhale and inhale, make the actuating lever noise reduction and alleviate the vibration when rotating the operation, reduce the test tube and lead to the impaired risk of test tube because of the vibrations power is too big in centrifugation in-process, the connecting plate drives the atress board at the liquid internal rotation through first pneumatic telescopic link, the first pneumatic telescopic link of atress extrusion of atress board, the air in the first pneumatic telescopic link passes through air guide groove and hose entering second pneumatic telescopic link, the second pneumatic telescopic link is tensile and promotes spacing fixture block and removes in the movable slot, make spacing fixture block push the test tube top in the standing groove, fix a position the test tube top, effectively avoid because the impaired condition of test tube takes place because of vibrations power is too big, the stability of test tube use has been guaranteed.
2. The centrifugal equipment and the method for detecting the cell protein by the PCR Array technology generate a pulling force on a pulling rope by turning a box cover, the pulling rope drives a sliding block to move upwards in a sliding groove, the sliding block drives a moving ring to move upwards outside a driving rod through a connecting rod, a third elastic element is stretched, the moving ring pulls a second ring body through a swinging rod in the moving-up process, the second ring body drives a lower tube body to move upwards relative to the upper tube body, the first elastic element is compressed, the second ring body drives the lower tube body to rotate by taking a connecting pin shaft of the first ring body and a connecting pin shaft of a placing groove as the circle center, the placing frame and the placing groove are in a vertical state, test tubes are installed in different placing frames respectively, the placing frames are shallow and form a straight line with the placing groove, the test tubes can be placed into the placing frames rapidly, the placing and taking out of the test tubes at the test tube positions can be rapidly realized, and the centrifugal efficiency is greatly improved.
3. According to the centrifugal equipment and the centrifugal method for detecting cell proteins by using the PCR Array technology, the driven bevel gear on the outer side is meshed with the fixed bevel gear on the fixed plate after the placing rack deflects, when the driving rod drives the placing rack to rotate through the rotating plate, the driven bevel gear on the placing rack can be meshed with the fixed bevel gear and then rotate, and the centrifugal effect on the test tube is further improved.
4. According to the centrifugal device and the method for detecting the cell protein by using the PCR Array technology, when the test tube is pushed by the limiting clamping block, the test tube compresses the bottom cavity of the rubber pad in the lower tube body, air in the bottom cavity enters the side cavity through the communicating groove, the side cavity expands to compress the side wall of the test tube, the test tube in centrifugation is efficiently fixed, and the use stability of the test tube is ensured.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a first schematic sectional view of the case according to the present invention;
FIG. 3 is a partially enlarged view of the portion A in FIG. 2 according to the present invention;
FIG. 4 is a schematic view of a portion B of FIG. 2 according to the present invention;
FIG. 5 is a schematic sectional view of the case of the present invention;
figure 6 is a schematic structural view of the rack of the present invention;
FIG. 7 is a schematic view of the outer structure of the shift ring of the present invention;
fig. 8 is a schematic sectional view of the rack of the present invention;
FIG. 9 is a schematic cross-sectional view of the lower tube body according to the present invention;
FIG. 10 is a cross-sectional view of the ear plate of the present invention;
fig. 11 is a schematic cross-sectional view of the first pneumatic telescopic rod of the present invention.
In the figure: 1. a box body; 101. a fixing plate; 102. a chute; 2. a box cover; 3. a rotating plate; 301. a placement groove; 4. a test tube; 5. a drive rod; 6. a drive device; 7. a housing; 701. rotating the tube; 702. a connecting plate; 703. a first pneumatic telescopic rod; 704. a stress plate; 8. placing a rack; 801. a pipe body is arranged; 8011. a first ring body; 802. a lower pipe body; 8021. a second ring body; 803. accommodating grooves; 804. a first elastic element; 9. an ear plate; 901. a movable groove; 902. a second pneumatic telescopic rod; 903. a limiting clamping block; 10. a sleeve; 1001. a piston; 1002. a second elastic element; 1003. a movable rod; 11. a gas guide groove; 12. a moving ring; 121. a swing lever; 122. a third elastic element; 123. a connecting rod; 124. a slider; 125. pulling a rope; 13. a rubber pad; 131. a bottom cavity; 132. a lateral cavity; 133. a communicating groove; 14. fixing a bevel gear; 141. a driven bevel gear.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", and the like, are used in the orientations and positional relationships indicated in the drawings, which are based on the orientations and positional relationships indicated in the drawings, and are used for convenience of description and simplicity of description, but do not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, are not to be construed as limiting the present invention.
Example 1:
referring to fig. 1, fig. 2, fig. 5 and fig. 6, a centrifugal device for detecting cell protein is used in PCR Array technique, including box 1 and box lid 2 of hinged joint at 1 top of box, fixed plate 101 has set firmly in the box 1, the fixed plate 101 internal rotation is connected with rotor plate 3, be provided with the standing groove 301 that is circumference evenly distributed on the rotor plate 3, be provided with rack 8 in the standing groove 301, test tube 4 has been placed on the rack 8, fixedly connected with actuating lever 5 on the rotor plate 3, be provided with in the box 1 and be used for driving 5 pivoted drive device 6 of actuating lever, the drive device 6 outside is provided with inhales the shake subassembly, still be provided with on the rack 8 with inhale shake subassembly matched with from locating component.
It is specific, during equipment uses, place test tube 4 in proper order and get into rack 8 in standing groove 301, begin to carry out centrifugal operation to test tube 4 afterwards, through control drive equipment 6 operation, drive equipment 6 can be for motor or motor etc. can drive 5 pivoted equipment of actuating lever, it is rotatory to make drive equipment 6 drive actuating lever 5, actuating lever 5 drives the rack 8 rotation on rotor plate 3 and the rotor plate 3, at this in-process, the vibration-absorbing assembly work, make actuating lever 5 noise reduction and alleviate vibration when rotating the operation, reduce test tube 4 because of the too big impaired risk of shaking force leading to test tube 4 in centrifugal process, and the vibration-absorbing assembly drives from locating component work, fix a position test tube 4 top, effectively avoid because the too big impaired condition emergence of test tube 4 that leads to of shaking force, the stability that test tube 4 used has been guaranteed, and then guarantee centrifugal effect.
Example 2:
referring to fig. 2, 5 and 11, in a centrifugal apparatus for detecting cell protein by using PCR Array technology, in embodiment 1, further, the vibration absorbing assembly includes a housing 7 fixed inside the casing 1 and covering the outside of the driving apparatus 6, the inner wall of the housing 7 is rotatably connected to a rotating tube 701, a storage cavity is formed between the housing 7 and the rotating tube 701, the storage cavity is filled with liquid, the rotating tube 701 is fixedly connected to the driving rod 5, a plurality of connecting plates 702 are arranged outside the rotating tube 701, a first pneumatic telescopic rod 703 is connected to the connecting plates 702, and a stressed plate 704 is connected to one end of the first pneumatic telescopic rod 703, which is far away from the connecting plates 702.
Specifically, when actuating lever 5 rotated, actuating lever 5 drove connecting plate 702 at casing 7 internal rotation through rotating tube 701, utilized the damping characteristic buffering of liquid self to inhale and shakes, made actuating lever 5 noise reduction and alleviate vibration when rotating the operation, reduced test tube 4 and too big the impaired risk of test tube 4 that leads to because of the shaking force at centrifugal in-process.
Example 3:
referring to fig. 2, fig. 3, fig. 5, fig. 6, fig. 10 and fig. 11, in a centrifugal apparatus for detecting cell protein by using PCR Array technology, on the basis of embodiment 2, further, the self-positioning assembly includes an ear plate 9 fixedly disposed on the placing frame 8, a movable groove 901 is disposed on the ear plate 9, a second pneumatic telescopic rod 902 is disposed on an inner wall of the movable groove 901, a limiting block 903 is connected to one end of the second pneumatic telescopic rod 902, which is far away from the inner wall of the movable groove 901, and an extrusion slope is disposed on the limiting block 903.
Further, the first pneumatic telescopic rod 703 and the second pneumatic telescopic rod 902 have the same structure and both include sleeves 10, the two sleeves 10 are connected to the inner walls of the connecting plate 702 and the movable groove 901 respectively, a piston 1001 is slidably connected to each sleeve 10, a second elastic element 1002 is disposed between the piston 1001 and the inner wall of the sleeve 10, and a movable rod 1003 is connected to one side of the piston 1001 away from the second elastic element 1002.
Further, the self-positioning assembly further comprises an air guide groove 11 formed in the connecting plate 702, the driving rod 5 and the rotating plate 3, one end of the air guide groove 11 is connected with the first pneumatic telescopic rod 703, and the other end of the air guide groove 11 is connected with the second pneumatic telescopic rod 902 through a hose.
Specifically, actuating lever 5 passes through rotating tube 701 and drives connecting plate 702 at casing 7 internal rotation, connecting plate 702 drives atress board 704 at the liquid internal rotation through first pneumatic telescopic link 703, first pneumatic telescopic link 703 of atress extrusion of atress board 704 atress, the air in first pneumatic telescopic link 703 passes through air guide slot 11 and hose entering second pneumatic telescopic link 902, second pneumatic telescopic link 902 is tensile and promotes spacing fixture block 903 and removes in movable groove 901, make spacing fixture block 903 push 4 tops of test tube in to standing groove 301, fix a position 4 tops of test tube, avoid test tube 4 to receive centrifugal force and fly out when the centrifugation, the stability of test tube 4 use has been guaranteed.
Example 4:
referring to FIGS. 1, 2, 3, 4, 5, 6, 7 and 8, in a centrifugal apparatus for detecting cell proteins according to the PCR Array technique, in example 1, a rack 8 includes an upper tube 801 and a lower tube 802 slidably connected to each other, a receiving groove 803 for allowing the upper tube 801 to slide is provided in the lower tube 802, and a first elastic member 804 is provided between the inner wall of the receiving groove 803 and the bottom of the upper tube 801.
Further, the outer sides of the upper pipe 801 and the lower pipe 802 are respectively rotatably connected with a first ring 8011 and a second ring 8021, the first ring 8011 is rotatably connected in the placement groove 301 through a pin shaft, the second ring 8021 is movably connected with a swing rod 121, one end of the swing rod 121 far away from the second ring 8021 is movably connected with a moving ring 12, the moving ring 12 is sleeved on the outer side of the driving rod 5, the outer side of the driving rod 5 is further sleeved with a third elastic element 122, two ends of the third elastic element 122 are respectively connected with the moving ring 12 and the shell 7, the outer side of the moving ring 12 is connected with a connecting rod 123, a slider 124 is fixedly connected to the connecting rod 123, a sliding groove 102 for sliding of the slider 124 is formed in the box 1, and a pull rope 125 is connected between the slider 124 and the box cover 2.
Specifically, when the device is used, the box cover 2 is opened, the box cover 2 is turned over relative to the box body 1, when the box cover 2 is turned over, a pulling force is generated on the pulling rope 125, the pulling rope 125 drives the slider 124 to move upwards in the sliding groove 102, the slider 124 drives the moving ring 12 to move upwards outside the driving rod 5 through the connecting rod 123, the third elastic element 122 is stretched, the moving ring 12 pulls the second ring 8021 through the swinging rod 121 in the moving upwards process, the second ring 8021 drives the lower tube body 802 to move upwards relative to the upper tube body 801, the first elastic element 804 is compressed, the second ring 8021 drives the lower tube body 802 to rotate around the connecting pin shaft of the first ring 8011 and the placing groove 301, the placing frame 8 and the placing groove 301 are in a vertical state, and then the test tubes 4 are respectively installed in different placing frames 8, because rack 8 is more shallow and is a straight line with standing groove 301, can put into rack 8 with test tube 4 fast, upset case lid 2 afterwards, make case lid 2 reclose at 1 top of box, case lid 2 no longer produces the pulling force to stay cord 125, shift ring 12 resets under the elasticity pulling of third elastic element 122 and moves down, shift ring 12 passes through swinging arms 121 and drives second ring 8021 and remove, second ring 8021 drives down the relative standing groove 301 deflection of body 802, and lower body 802 slides relatively to last body 801, make rack 8 can hold more extensive test tube 4, the area that test tube 4 spills rack 8 diminishes, with the protection and the fixed effect to test tube 4 that improve, can realize test tube 4 fast and put into and take out at 4 positions of test tube, improve centrifugal efficiency greatly.
Example 5:
referring to fig. 2 and 9, in a centrifugal apparatus for cell protein detection according to the PCR Array technique, in example 4, a rubber pad 13 is disposed on an inner wall of a lower tube 802, a bottom chamber 131 and a side chamber 132 are disposed in the rubber pad 13, and a communication groove 133 is disposed between the bottom chamber 131 and the side chamber 132.
Specifically, when pushing test tube 4 through spacing fixture block 903, test tube 4 oppresses bottom cavity 131 of rubber pad 13 in the body 802 down, and the air in bottom cavity 131 gets into side chamber 132 through intercommunication groove 133, and side chamber 132 inflation makes rubber pad 13 oppress the test tube 4 lateral wall, to test tube 4 high-efficient fixed in the centrifugal, has guaranteed the stability that test tube 4 used.
Example 6:
referring to fig. 2, 3, 5 and 6, in a centrifugal apparatus for cell protein detection in the PCR Array technique, in addition to embodiment 4, a fixed bevel gear 14 is fixedly connected to a fixed plate 101, and a driven bevel gear 141 engaged with the fixed bevel gear 14 is provided on a rack 8.
Specifically, 2 lids of case lid close the back, and rack 8 deflects and the slope of standing groove 301 sets up, and driven bevel gear 141 in the rack 8 outside meshes with fixed bevel gear 14 on the fixed plate 101, and when actuating lever 5 passed through rotor plate 3 and drives rack 8 and rotate, driven bevel gear 141 on the rack 8 can mesh the back rotation with fixed bevel gear 14, makes down pipe 802 and upper pipe body 801 at two internal rotations of rings respectively, further improves the centrifugal effect to test tube 4.
The invention also discloses a centrifugal method for detecting cell protein by using the PCR Array technology, which comprises the centrifugal equipment for detecting cell protein by using the PCR Array technology, and also comprises the following steps:
s1: when the device is used, the box cover 2 is opened, the box cover 2 is turned over relative to the box body 1, when the box cover 2 is turned over, a pulling force is generated on the pulling rope 125, the pulling rope 125 drives the sliding block 124 to move upwards in the sliding groove 102, the sliding block 124 drives the moving ring 12 to move upwards outside the driving rod 5 through the connecting rod 123, the third elastic element 122 is stretched, the moving ring 12 is pulled to the second ring body 8021 through the swinging rod 121 in the moving-up process, the second ring body 8021 drives the lower pipe body 802 to move upwards relative to the upper pipe body 801, the first elastic element 804 is compressed, and the second ring body 8021 drives the lower pipe body 802 to rotate by taking the connecting pin shaft of the first ring body 8011 and the placing groove 301 as the circle center, so that the placing rack 8 and the placing groove 301 are in a vertical state, and then the test tubes 4 are respectively installed in different placing racks 8, and the placing racks 8 are shallow and form a straight line with the placing groove 301, so that the test tubes 4 can be quickly placed in the placing racks 8;
s2: then, the box cover 2 is turned over, so that the box cover 2 is closed on the top of the box body 1 again, the box cover 2 does not produce pulling force on the pull rope 125 any more, the moving ring 12 is pulled by the elastic force of the third elastic element 122 to reset and move downwards, the moving ring 12 drives the second ring 8021 to move through the swing rod 121, the second ring 8021 drives the lower tube body 802 to deflect relative to the placing groove 301, the lower tube body 802 slides relative to the upper tube body 801, the placing frame 8 can accommodate test tubes 4 with a larger area, and the area of the test tubes 4 leaking out of the placing frame 8 is reduced, so that the protection and fixing effects on the test tubes 4 are improved;
s3: the driven bevel gear 141 on the outer side of the deflected placing frame 8 is meshed with the fixed bevel gear 14 on the fixed plate 101, when the driving rod 5 drives the placing frame 8 to rotate through the rotating plate 3, the driven bevel gear 141 on the placing rack 8 can be meshed with the fixed bevel gear 14 and then can rotate, so that the centrifugal effect on the test tube 4 is further improved;
s4: then, the driving device 6 is controlled to operate, so that the output end of the driving device 6 drives the driving rod 5 to rotate, the driving rod 5 drives the placing rack 8 at the placing groove 301 to rotate through the rotating plate 3, and further, the cells in the test tubes 4 are subjected to centrifugal operation;
s5: when the driving rod 5 rotates, the driving rod 5 drives the connecting plate 702 to rotate in the shell 7 through the rotating pipe 701, the damping characteristic of liquid is utilized to buffer and absorb vibration, so that noise and vibration are reduced when the driving rod 5 rotates, the risk that the test tube 4 is damaged due to overlarge vibration force in the centrifugal process is reduced, the connecting plate 702 drives the stress plate 704 to rotate in the liquid through the first pneumatic telescopic rod 703, the stress plate 704 is stressed to extrude the first pneumatic telescopic rod 703, air in the first pneumatic telescopic rod 703 enters the second pneumatic telescopic rod 902 through the air guide groove 11 and the hose, the second pneumatic telescopic rod 902 stretches and pushes the limiting clamping block 903 to move in the movable groove 901, and the limiting clamping block 903 pushes the top of the test tube 4 in the placing groove 301 to position the top of the test tube 4;
s6: in the process that limiting clamping block 903 pushes test tube 4, test tube 4 presses bottom cavity 131 of rubber pad 13 in lower tube body 802, air in bottom cavity 131 enters side cavity 132 through communicating groove 133, and side cavity 132 expands to press the side wall of test tube 4 by rubber pad 13, so that test tube 4 in the centrifugal process is efficiently fixed.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
Claims (10)
1. The utility model provides a PCR Array technique is at cell protein centrifugal device for detection, includes case (1) and hinged joint case lid (2) at case (1) top, its characterized in that, fixed plate (101) have set firmly in case (1), fixed plate (101) internal rotation is connected with rotor plate (3), be provided with standing groove (301) that are circumference evenly distributed on rotor plate (3), be provided with rack (8) in standing groove (301), test tube (4) have been placed on rack (8), fixedly connected with actuating lever (5) on rotor plate (3), be provided with in case (1) and be used for driving actuating lever (5) pivoted drive device (6), the shock absorption subassembly is provided with in drive device (6) outside, still be provided with on rack (8) and inhale shock absorption subassembly matched with from positioning assembly.
2. The centrifugal device for detecting cell protein by using the PCR Array technology according to claim 1, wherein the vibration absorption assembly comprises a housing (7) which is fixedly arranged inside the box body (1) and covers the outside of the driving device (6), a rotating tube (701) is rotatably connected to the inner wall of the housing (7), a storage cavity is formed between the housing (7) and the rotating tube (701), liquid is filled in the storage cavity, the rotating tube (701) is fixedly connected with the driving rod (5), a plurality of connecting plates (702) are arranged on the outside of the rotating tube (701), a first pneumatic telescopic rod (703) is connected to each connecting plate (702), and one end, far away from the connecting plates (702), of the first pneumatic telescopic rod (703) is connected with a stressed plate (704).
3. The centrifugal device for detecting cell protein by using the PCR Array technology according to claim 2, wherein the self-positioning assembly comprises an ear plate (9) fixedly arranged on the placing frame (8), the ear plate (9) is provided with a movable groove (901), the inner wall of the movable groove (901) is provided with a second pneumatic telescopic rod (902), one end of the second pneumatic telescopic rod (902), which is far away from the inner wall of the movable groove (901), is connected with a limiting clamping block (903), and the limiting clamping block (903) is provided with an extrusion inclined surface.
4. The centrifugal device for detecting cell protein by using the PCR Array technology according to claim 3, wherein the first pneumatic telescopic rod (703) and the second pneumatic telescopic rod (902) have the same structure and respectively comprise a sleeve (10), the two sleeves (10) are respectively connected with the connecting plate (702) and the inner wall of the movable groove (901), a piston (1001) is slidably connected in each sleeve (10), a second elastic element (1002) is arranged between the piston (1001) and the inner wall of each sleeve (10), and a movable rod (1003) is connected to one side of the piston (1001) away from the second elastic element (1002).
5. The centrifugal apparatus for detecting cell protein according to the PCR Array technology of claim 3, wherein the self-positioning assembly further comprises an air guide groove (11) formed in the connecting plate (702), the driving rod (5) and the rotating plate (3), one end of the air guide groove (11) is connected to the first pneumatic telescopic rod (703), and the other end of the air guide groove (11) is connected to the second pneumatic telescopic rod (902) through a hose.
6. The centrifugal apparatus for detecting cell protein according to claim 1, wherein the rack (8) comprises an upper tube (801) and a lower tube (802) which are slidably connected, the lower tube (802) is provided with a holding groove (803) for the upper tube (801) to slide, and a first elastic element (804) is disposed between the inner wall of the holding groove (803) and the bottom of the upper tube (801).
7. The centrifugal apparatus for detecting cell protein according to the PCR Array technology of claim 6, wherein the upper tube body (801) and the lower tube body (802) are respectively and rotatably connected with a first ring body (8011) and a second ring body (8021) at outer sides thereof, the first ring body (8011) is rotatably connected in the placement groove (301) through a pin shaft, the second ring body (8021) is movably connected with a swing rod (121), one end of the swing rod (121) away from the second ring body (8021) is movably connected with a movable ring (12), the movable ring (12) is sleeved on an outer side of the driving rod (5), the outer side of the driving rod (5) is further sleeved with a third elastic element (122), two ends of the third elastic element (122) are respectively connected with the movable ring (12) and the housing (7), the outer side of the movable ring (12) is connected with a connecting rod (123), the connecting rod (123) is fixedly connected with a slider (124), a sliding groove (102) for sliding the slider (124) is formed in the housing (1), and a pull rope (125) is connected between the slider (124) and the case cover (2).
8. The centrifugal apparatus for detecting cell protein according to claim 7, wherein the rubber pad (13) is disposed on the inner wall of the lower tube (802), the rubber pad (13) has a bottom chamber (131) and a side chamber (132) therein, and a communication groove (133) is disposed between the bottom chamber (131) and the side chamber (132).
9. The centrifugal apparatus for detecting cell protein according to the PCR Array technology of claim 1, wherein a fixed bevel gear (14) is fixedly connected to the fixed plate (101), and a driven bevel gear (141) engaged with the fixed bevel gear (14) is disposed on the rack (8).
10. A centrifugal method for detecting a cellular protein by a PCR Array technique, comprising the centrifugal apparatus for detecting a cellular protein by a PCR Array technique according to any one of claims 1 to 9, further comprising the steps of:
s1: when the device is used, the box cover (2) is opened, the box cover (2) is turned over relative to the box body (1), a pulling force is generated on a pulling rope (125) when the box cover (2) is turned over, the pulling rope (125) drives the sliding block (124) to move upwards in the sliding groove (102), the sliding block (124) drives the moving ring (12) to move upwards outside the driving rod (5) through the connecting rod (123), the third elastic element (122) is stretched, the moving ring (12) moves upwards in the process of pulling the second ring body (8021) through the swinging rod (121), the second ring body (8021) drives the lower tube body (802) to move upwards relative to the upper tube body (801), the first elastic element (804) is compressed, the second ring body (8021) drives the lower tube body (802) to rotate by taking the connecting pin shaft of the first ring body (8011) and the placing groove (301) as the circle center, the placing rack (8) and the placing groove (301) can be placed in a straight line (8);
s2: then, the box cover (2) is turned over, the box cover (2) is closed at the top of the box body (1) again, the box cover (2) does not generate pulling force on the pulling rope (125), the moving ring (12) is reset and moves downwards under the pulling of the elastic force of the third elastic element (122), the moving ring (12) drives the second ring body (8021) to move through the swinging rod (121), the second ring body (8021) drives the lower tube body (802) to deflect relative to the placing groove (301), and the lower tube body (802) slides relative to the upper tube body (801), so that the placing rack (8) can contain test tubes (4) with larger area, the area of the placing rack (8) of the test tubes (4) is reduced, and the protection and fixing effects on the test tubes (4) are improved;
s3: the driven bevel gear (141) on the outer side of the deflected placing rack (8) is meshed with the fixed bevel gear (14) on the fixed plate (101), and when the driving rod (5) drives the placing rack (8) to rotate through the rotating plate (3), the driven bevel gear (141) on the placing rack (8) can be meshed with the fixed bevel gear (14) and then rotate;
s4: then controlling the driving equipment (6) to operate, enabling the output end of the driving equipment (6) to drive the driving rod (5) to rotate, and driving the driving rod (5) to drive the placing rack (8) at the placing groove (301) to rotate through the rotating plate (3), so as to carry out centrifugal operation on the cells in the test tube (4);
s5: when the driving rod (5) rotates, the driving rod (5) drives the connecting plate (702) to rotate in the shell (7) through the rotating pipe (701), damping and vibration absorption are achieved by utilizing the damping characteristic of liquid, noise and vibration are reduced when the driving rod (5) rotates, the risk that the test tube (4) is damaged due to overlarge vibration force in the centrifugal process of the test tube (4) is reduced, the connecting plate (702) drives the stress plate (704) to rotate in the liquid through the first pneumatic telescopic rod (703), the stress plate (704) extrudes the first pneumatic telescopic rod (703) in a stressed mode, air in the first pneumatic telescopic rod (703) enters the second pneumatic telescopic rod (902) through the air guide groove (11) and the hose, the second pneumatic telescopic rod (902) stretches and pushes the limiting clamping block (903) to move in the movable groove (901), and the limiting clamping block (903) pushes the top of the test tube (4) in the placement groove (301), and the top of the test tube (4) is positioned;
s6: in-process that spacing fixture block (903) pushed to test tube (4), test tube (4) oppresses bottom chamber (131) of rubber pad (13) in lower body (802), and the air in bottom chamber (131) gets into side chamber (132) through intercommunication groove (133), and side chamber (132) inflation makes rubber pad (13) oppress test tube (4) lateral wall, to test tube (4) high efficiency fixed in the centrifugal.
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